CN104593257A - Three-dimensional cell assembly device and method based on movable electrode - Google Patents
Three-dimensional cell assembly device and method based on movable electrode Download PDFInfo
- Publication number
- CN104593257A CN104593257A CN201510082367.7A CN201510082367A CN104593257A CN 104593257 A CN104593257 A CN 104593257A CN 201510082367 A CN201510082367 A CN 201510082367A CN 104593257 A CN104593257 A CN 104593257A
- Authority
- CN
- China
- Prior art keywords
- cell
- needle tubing
- conductive
- power supply
- metal layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a three-dimensional cell assembly device based on a movable electrode. The device comprises a conductive bottom plate, a conductive needle tube coating the conductive bottom plate with a mixed solution, a liquid injection device and a needle tube moving mechanism or a bottom plate moving mechanism, wherein the conductive needle tube comprises an inner sleeve tube and a conductive metal deposition layer covering an outer side wall of the inner sleeve tube; the conductive bottom plate and the conductive metal deposition layer are connected with two power supply output ends of a current controller respectively. The device is simple in structure, reasonable in design, easy and convenient to use and operate and good in use effect, and can simply and conveniently adjust the shape of hydrogel containing cells. Meanwhile, the invention further discloses a three-dimensional cell assembly method based on the movable electrode. The method comprises the following steps: 1, conveying the mixed solution; 2, performing water electrolysis and generating the hydrogel; 3, adjusting the position of the conductive needle tube and adjusting water electrolysis current. The method is simple in step, convenient to realize and good in use effect, and the hydrogel shape is adjusted by controlling the position of the movable electrode and the current.
Description
Technical field
The invention belongs to Biotechnology field, especially relate to a kind of cell three-dimensional apparatus for assembling based on travelling electrode and method.
Background technology
A key issue that will solve in biotechnology how biomass cells is assembled into have bioactive artificial substituent, in order to maintain, to recover or improve the function of tissue.How to grow into functional histoorgan to understand cell better, existing method is generally studied the growth characteristics of cell by culturing cell on culture dish.In recent years, increasing research shows, the growth conditions of cell on two dimensional surface can not the growing environment of reacting cells in human body truly.Therefore, how biomass cells be fixed on three-dimensional space and allow its growth be a gordian technique in biotechnology, fix by biomass cells and form the three-dimensional space fixed sturcture of a biomass cells.
Sodium alginate (Sodium Alginate) is a kind of natural polysaccharide, has stability, solvability and nontoxicity, is widely used in the field such as food-processing, pharmaceutical preparation.By adding CaCl in containing the sodium alginate of biomass cells
2solution, sodium alginate meeting and Ca
2+react and generate a kind of hydrogel: alginate calcium (Calcium Alginate).It is simple that this hydrogel has preparation, and the advantages such as bio-compatibility is good, being extensively used as biological support fixes cell, realizes cell in three-dimensional cultivation.But, in traditional preparation method, Ca
2+concentration and be distributed in mixing solutions be difficult to controlled.Therefore, the hydrogel shape containing cell is also difficult to accurate control, causes cell to be also difficult to control in three-dimensional growth.
Summary of the invention
Technical problem to be solved by this invention is for above-mentioned deficiency of the prior art, a kind of cell three-dimensional apparatus for assembling based on travelling electrode is provided, its structure simple, reasonable in design and use easy and simple to handle, result of use good, internal celliferous hydrogel shape can carry out easy adjustment, and hydrogel shape is easy to control.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of cell three-dimensional apparatus for assembling based on travelling electrode, it is characterized in that: comprise conductive soleplate, be positioned at conduction needle tubing above conductive soleplate, the cell stationary liquid of embedded biometric cell be delivered to the priming device of described conduction needle tubing and drive the needle tubing travel mechanism of described conduction needle tubing movement above conductive soleplate, described conduction needle tubing to be arranged in described needle tubing travel mechanism; Described conduction needle tubing comprises inner sleeve and covers the conductive metal layer on inner sleeve outer side wall, and the upper end of described inner sleeve has fluid inlet and its lower end has liquid outlet, and described priming device is connected with the fluid inlet of inner sleeve by pipe connecting; Described cell stationary liquid is by the aqueous solution of sodium alginate, calcium carbonate and water Homogeneous phase mixing, and in the described aqueous solution, the weight percentage of sodium alginate is 1% ~ 4%, and in the described aqueous solution, the weight percentage of calcium carbonate is 0.1% ~ 0.4%; Described conductive soleplate and conductive metal layer connect with two power output ends of DC power supply unit respectively, described conductive soleplate, conductive metal layer and DC power supply unit composition carries out electrolysis to described cell stationary liquid and generates the electrolyzer of hydrogel, and described conductive soleplate and conductive metal layer are electrode for electrolysis; Described conduction needle tubing is the coating unit cell stationary liquid of embedded biometric cell or the hydrogel of embedded biometric cell are coated on conductive soleplate.
Above-mentioned a kind of cell three-dimensional apparatus for assembling based on travelling electrode, it is characterized in that: described DC power supply unit is the adjustable adjustable DC power supply of current value, and described priming device and/or described pipe connecting are equipped with the flow regulation device regulated the cell stationary liquid flow of the embedded biometric cell in described pipe connecting.
Above-mentioned a kind of cell three-dimensional apparatus for assembling based on travelling electrode, is characterized in that: described conductive soleplate is that level is laid, and described conduction needle tubing is vertically to laying.
Above-mentioned a kind of cell three-dimensional apparatus for assembling based on travelling electrode, it is characterized in that: described priming device is syringe, described inner sleeve is glass needle tubing.
Above-mentioned a kind of cell three-dimensional apparatus for assembling based on travelling electrode, it is characterized in that: the middle and upper part of described inner sleeve is cylindrical tube and its underpart is diameter diminishing conical tube from top to bottom, and the upper end diameter of described conical tube is identical with the diameter of described cylindrical tube and its bottom is described liquid outlet; The bore of described liquid outlet is Φ 50 μm ~ Φ 200 μm.
Above-mentioned a kind of cell three-dimensional apparatus for assembling based on travelling electrode, it is characterized in that: described needle tubing travel mechanism is mechanical arm, described mechanical arm comprises base, the column be arranged on described base, be fixed on the sway brace on described column and be arranged on the clamping device clamped on described sway brace and to described conduction needle tubing top.
Above-mentioned a kind of cell three-dimensional apparatus for assembling based on travelling electrode, it is characterized in that: the middle and lower part outer side wall of described inner sleeve is all coated with conductive metal layer, described conductive metal layer is metal deposition layer.
Above-mentioned a kind of cell three-dimensional apparatus for assembling based on travelling electrode, is characterized in that: described metal deposition layer is deposition of gold layer.
Above-mentioned a kind of cell three-dimensional apparatus for assembling based on travelling electrode, is characterized in that: positive supply output terminal or the negative supply output terminal of described conductive metal layer and DC power supply unit are connected; When the positive supply output terminal of conductive metal layer and DC power supply unit, described conductive soleplate is connected with the negative supply output terminal of DC power supply unit, now conductive metal layer is the anode of described electrolyzer, and described conduction needle tubing is the coating unit be coated in by the hydrogel of embedded biometric cell on conductive soleplate; When the negative supply output terminal of conductive metal layer and DC power supply unit, described conductive soleplate is connected with the positive supply output terminal of DC power supply unit, now conductive soleplate is the anode of described electrolyzer, and described conduction needle tubing is the coating unit be coated in by the cell stationary liquid of embedded biometric cell on conductive soleplate.
Simultaneously, the invention also discloses a kind of method steps simple, realize convenient and result of use is good, by the cell three-dimensional assemble method based on travelling electrode of the size adjustment hydrogel shape of the position and/or electric current that control traveling electrode, it is characterized in that the method comprises the following steps:
Step one, electrolyzer wiring: by conductive soleplate and conductive metal layer, connect with two power output ends of DC power supply unit respectively;
Step 2, cell stationary liquid are carried: after biomass cells being put into described biological fixation liquid, send in described conduction needle tubing by priming device by the biological fixation liquid of embedded biometric cell;
Step 3, the adjustment of conduction syringe location and Faradaic current adjustment: open DC power supply unit, and according to the biomass cells designed in advance the three-dimensional profiles fixed, adjust by the position of described needle tubing travel mechanism to described conduction needle tubing and/or the current value of DC power supply unit is adjusted, just obtain the three-dimensional space fixed sturcture of biomass cells; Described three-dimensional space fixed sturcture is be positioned on conductive soleplate and the hydrogel of embedded biometric cell.
The present invention compared with prior art has the following advantages:
1, the cell three-dimensional apparatus for assembling adopted, simple, the reasonable in design and easy accessibility of structure, input cost is lower.
2, the cell three-dimensional apparatus for assembling adopted uses easy and simple to handle and result of use good, internal celliferous hydrogel shape can carry out easy adjustment, and the shape of interior celliferous hydrogel (i.e. the three-dimensional space fixed sturcture of biomass cells) is easy to control.During actual use, based on the principle of galvanic deposit, by controlling the position of traveling electrode and the size (i.e. the intensity of electric field) of electric current, accurately control Ca
2+the position produced and concentration, thus the shape controlling the hydrogel containing biomass cells, and the accurate control of hydrogel shape can be realized, finally realize the three-dimensional assembling of cell.
3, use flexible operation mode, the needle tubing that both can conduct electricity is arranged in needle tubing travel mechanism, and using conductive metal layer as traveling electrode, conductive soleplate is as fixed electorde; Also using conduction needle tubing as fixed electorde, the position of conductive soleplate can be moved.That is, only need there is relative movement between conductive soleplate and conduction needle tubing.During actual use, be energized on the conductive metal layer and conductive soleplate of conduction needle tubing by DC power supply unit, just can generate alginate calcium at anode through galvanic deposit, in alginate calcium generative process, biomass cells can be fixed in hydrogel.Wherein, conductive soleplate both can make anode, also can make negative electrode; Correspondingly, the conductive metal layer of conduction needle tubing both can make negative electrode, also can make anode.
4, the cell three-dimensional assemble method step adopted is simple, realization is convenient and result of use is good, realize cell three-dimensional assembling by celliferous hydrogel shape in traveling electrode adjustment.Further, have that bio-compatibility is good, processing step be simple, 3D shape and structure-controllable, control accuracy advantages of higher.
During actual use, based on principles of electro-deposition, the cell stationary liquid containing biomass cells is injected conduction needle tubing by syringe, when powering up at conduction needle tubing and conductive soleplate, can at anodic formation hydrogel; Controlled the position of conduction needle tubing liquid outlet by mechanical arm, controlled the size of strength of electric field by DC power supply unit, realize the accurate control to hydrogel shape, finally realize the three-dimensional assembling of cell.In actual use procedure, the liquid outlet of conduction needle tubing is near conductive soleplate, but the two does not contact; The grout that the inside of final generation is fixed with biomass cells is laid on conductive soleplate.
Below by drawings and Examples, technical scheme of the present invention is described in further detail.
Accompanying drawing explanation
Fig. 1 is the using state reference drawing of cell three-dimensional apparatus for assembling of the present invention.
Description of reference numerals:
1-mechanical arm; 2-syringe; 3-inner sleeve;
3-1-conductive metal layer; 4-conductive soleplate; 5-DC power supply unit;
5-1-the first wire; 5-2-the second wire; 6-hydrogel.
Embodiment
The cell three-dimensional apparatus for assembling based on travelling electrode as shown in Figure 1, comprise conductive soleplate 4, be positioned at conduction needle tubing above conductive soleplate 4, the cell stationary liquid of embedded biometric cell be delivered to the priming device of described conduction needle tubing and drive the needle tubing travel mechanism of described conduction needle tubing movement above conductive soleplate 4, described conduction needle tubing is arranged in described needle tubing travel mechanism.The conductive metal layer 3-1 that described conduction needle tubing comprises inner sleeve 3 and covers on inner sleeve 3 outer side wall, the upper end of described inner sleeve 3 has fluid inlet and its lower end has liquid outlet, and described priming device is connected by the fluid inlet of pipe connecting with inner sleeve 3.Described cell stationary liquid is by the aqueous solution of sodium alginate, calcium carbonate and water Homogeneous phase mixing, and in the described aqueous solution, the weight percentage of sodium alginate is 1% ~ 4%, and in the described aqueous solution, the weight percentage of calcium carbonate is 0.1% ~ 0.4%.Described conductive soleplate 4 and conductive metal layer 3-1 connect with two power output ends of DC power supply unit 5 respectively, described conductive soleplate 4, conductive metal layer 3-1 and DC power supply unit 5 form and carry out electrolysis to described cell stationary liquid and generate the electrolyzer of hydrogel 6, and described conductive soleplate 4 and conductive metal layer 3-1 are electrode for electrolysis; Described conduction needle tubing is the coating unit cell stationary liquid of embedded biometric cell or the hydrogel 6 of embedded biometric cell are coated on conductive soleplate 4.
Biomass cells is stem cell.Briefly, it is the original undifferentiated cell that a class has multi-lineage potential and the of self-replication capacity, is the initiating cell of each histoorgan forming mammal.In order to keep the original morphological structure of cell better, preventing tissue automatic soup-dissolving, being necessary to be fixed cell.Cell after fixing is immobilized cell (Immobilized cells), refers to and is fixed on water insoluble carrier, certain spatial dimension carry out vital movement (growth, grow, breeding, heredity and metabolic etc.) cell.Thus, cell is exactly fixedly make its intracellular material as far as possible close to morphological structure during its animation and position, fixing object is to prevent histocyte self-dissolving with corrupt, prevent intracellular enzyme to the Decomposition of protein, similar during to keep the original structure of cell and position and animation.When being fixed cell, cell stationary liquid is usually adopted to be fixed.Be a kind of conventional and cell stationary liquid well known to those skilled in the art by the cell stationary liquid of sodium alginate, calcium carbonate and water Homogeneous phase mixing.
In the present embodiment, being delivered to the cell stationary liquid of the embedded biometric cell in described conduction needle tubing by described priming device, is the mixing solutions be made up of biomass cells and cell stationary liquid.Biomass cells contained in described cell stationary liquid is the biomass cells that need fix, and namely needs the biomass cells carrying out three-dimensional assembling.
In actual use procedure, the consumption of cell stationary liquid in described mixing solutions, determines the conventional fastening method that biomass cells is fixed according to employing cell stationary liquid.After in described mixing solutions, the consumption of cell stationary liquid is determined, the content of biomass cells in described mixing solutions, just can determine.
During actual use, the content of biomass cells in described mixing solutions, adjusts accordingly according to the requirement of different biomass cells to growing environment.
Thus, according to the conventional fastening method adopting cell stationary liquid to be fixed biomass cells, according to the requirement of different biomass cells to growing environment, and combine the biomass cells amount that need fix, the consumption of adopted cell stationary liquid is determined.
In actual use procedure, can according to specific needs, the weight percentage of sodium alginate and calcium carbonate in the described aqueous solution be adjusted accordingly.Wherein, described calcium carbonate is calcium carbonate microparticle, and the particle diameter of calcium carbonate microparticle is not more than 3 microns.
In the present embodiment, described DC power supply unit is the adjustable adjustable DC power supply of current value.
During actual use, described adjustable DC power supply is by DC power supply and connect with described DC power supply and the matrix current adjustment circuit adjusted the current value size of this DC power supply.
In the present embodiment, described conductive soleplate 4 is laid in level, and described conduction needle tubing is vertically to laying.
Further, described conduction needle tubing is arranged in described needle tubing travel mechanism, and conductive metal layer 3-1 is traveling electrode and conductive soleplate 4 is the fixed fixed electorde in position.
During actual use, also the position of described conduction needle tubing can be maintained static, move the position of conductive soleplate 4, now conductive soleplate 4 is traveling electrode, and conductive metal layer 3-1 is the fixed fixed electorde in position.
In the present embodiment, described conductive soleplate 4 is leveling board.Further, described conductive soleplate 4 and described conduction needle tubing are vertical runs.
Actual when using, can according to specific needs, described conduction needle tubing and the relative position of conductive soleplate 4 and the spacing between the liquid outlet of described conduction needle tubing and conductive soleplate 4 are adjusted; Further, also can according to actual needs, conductive soleplate 4 and the angle of inclination between described conduction needle tubing and horizontal plane be adjusted respectively.
In the present embodiment, described inner sleeve 3 is glass needle tubing.
During actual use, described inner sleeve 3 also can adopt the tubing of other non-conductive material.
In the present embodiment, the middle and lower part outer side wall of described inner sleeve 3 is all coated with conductive metal layer 3-1.Further, on described inner sleeve 3 outer side wall to cover the thickness of conductive metal layer 3-1 all identical.
Actually add man-hour, the middle and upper part of described inner sleeve 3 is cylindrical tube and its underpart is diameter diminishing conical tube from top to bottom, and the upper end diameter of described conical tube is identical with the diameter of described cylindrical tube and its bottom is described liquid outlet.The bore of described liquid outlet is Φ 50 μm ~ Φ 200 μm.
In the present embodiment, described priming device is syringe 2.The actual used time, can the mixing solutions flow flow on conductive soleplate 4 from described conduction needle tubing be adjusted by described syringe 2.
During actual use, described priming device also can adopt the liquid injecting apparatus of other type.
Meanwhile, the flow regulation device regulated the cell stationary liquid flow of the embedded biometric cell in described pipe connecting is equipped with described priming device and/or described pipe connecting.
Flow regulation device is equipped with in the present embodiment, described syringe 2.
During actual use, also on described pipe connecting, flow regulation device can be installed, and the flow regulation device installed is flow control valve.
In the present embodiment, described needle tubing travel mechanism is mechanical arm 1, and described mechanical arm 1 comprises base, the column be arranged on described base, be fixed on the sway brace on described column and be arranged on the clamping device clamped on described sway brace and to described conduction needle tubing top.
In the present embodiment, described base and described sway brace are all laid in level, and described column is in vertically to laying, and described priming device is arranged on described sway brace.
During actual use, also can according to specific needs, the installation position of described priming device be adjusted accordingly.
In the present embodiment, described conductive metal layer 3-1 is metal deposition layer.
Further, described metal deposition layer is deposition of gold layer.
Actual when using, described conductive metal layer 3-1 also can be the excellent metal deposition layer of other conductivity, as deposited silver layer, copper deposits, al deposition layer, nickel deposition layer etc.
Actual when carrying out wiring, positive supply output terminal or the negative supply output terminal of described conductive metal layer 3-1 and DC power supply unit 5 are connected; When the positive supply output terminal of conductive metal layer 3-1 and DC power supply unit 5, described conductive soleplate 4 is connected with the negative supply output terminal of current controller 5, now conductive metal layer 3-1 is the anode of described electrolyzer, and described conduction needle tubing is the coating unit be coated in by the hydrogel 6 of embedded biometric cell on conductive soleplate 4; When the negative supply output terminal of conductive metal layer 3-1 and DC power supply unit 5, described conductive soleplate 4 is connected with the positive supply output terminal of current controller 5, now conductive soleplate 4 is the anode of described electrolyzer, and described conduction needle tubing is the coating unit be coated in by the cell stationary liquid of embedded biometric cell on conductive soleplate 4.
The present invention is based upon in the principle of galvanic deposit, when adopting cell three-dimensional apparatus for assembling as shown in Figure 1 to carry out cell three-dimensional assembling, described mixing solutions enters described conduction needle tubing from syringe 2 by pipe connecting, and flows out on conductive soleplate 4 by the liquid outlet of described conduction needle tubing; All be connected DC power supply unit 5 as conductive metal layer 3-1 with conductive soleplate 4 and form electrolyzer after energising, by this electrolyzer, electrolysis being carried out to the water in described mixing solutions, and described electrolyzer anode generation water electrolysis reaction and generate H
+, reaction formula is as follows: 2H
2o=O
2↑+4H
++ 4e
-; The H that water electrolysis reaction generates
+react with the calcium carbonate microparticle in described mixing solutions and generate Ca
2+, reaction formula is as follows: 2H
++ CaCO
3=Ca
2++ H
2o+CO
2↑; The Ca generated
2+to react generation alginate calcium with sodium alginate, reaction formula is as follows: 2Ca
2++ 2Alg-COO
-=Alg-COO-Ca-OOC-Alg ↓, wherein Alg-COO
-for Lalgine, Alg-COO-Ca-OOC-Alg is alginate calcium (i.e. hydrogel 6).Thus, at the hydrogel 6 that the anode of described electrolyzer generates.
When conductive metal layer 3-1 is the anode of described electrolyzer, the cell stationary liquid of described conduction needle tubing middle and lower part generates hydrogel 6 after electrolysis, and the hydrogel 6 generated flow on conductive soleplate 4 through liquid outlet, completes the fixation procedure of biomass cells; When conductive soleplate 4 is the anode of described electrolyzer, the cell stationary liquid of embedded biometric cell flow on conductive soleplate 4 through the liquid outlet of described conduction needle tubing, and generate the hydrogel 6 of embedded biometric cell at conductive soleplate 4 By Electrolysis, complete the fixation procedure of biomass cells.
In the present embodiment, described conductive metal layer 3-1 is connected with the positive supply output terminal of DC power supply unit 5, and described conductive soleplate 4 is connected with the negative supply output terminal of DC power supply unit 5.
That is, described conductive metal layer 3-1 is anode, and described conductive soleplate 4 is negative electrode.
During physical cabling, also conductive metal layer 3-1 can be connected with the negative supply output terminal of DC power supply unit 5, described conductive soleplate 4 is connected with the positive supply output terminal of DC power supply unit 5.
In the present embodiment, the metal conducting layer that described conductive soleplate 4 comprises substrate and lays on the substrate.Wherein, described substrate is non-conducting material.Thus, described metal conducting layer is connected with the negative supply output terminal of DC power supply unit 5.The metal level that described metal conducting layer can adopt silver, copper, aluminium etc. to conduct electricity very well.
During physical cabling, described conductive metal layer 3-1 is connected with two power output ends of DC power supply unit 5 with the second wire 5-2 respectively by the first wire 5-1 with conductive soleplate 4.
In the present embodiment, described DC power supply unit 5 is the model that Beijing Zhong Taihua rising sun scientific & trading Co., Ltd. produces is the DC power supply unit of SOC-AH6-2, the electric current of this DC power supply unit energy constant output six road 20mA ~ 200mA and its input voltage is the alternating-current of 220V.
Adopt cell three-dimensional apparatus for assembling as shown in Figure 1 to carry out the method for cell three-dimensional assembling, comprise the following steps:
Step one, electrolyzer wiring: by conductive soleplate 4 and conductive metal layer 3-1, connect with two power output ends of DC power supply unit 5 respectively.
Step 2, cell stationary liquid are carried: after biomass cells being put into described biological fixation liquid, sent in described conduction needle tubing by the biological fixation liquid of embedded biometric cell by priming device.
Step 3, the adjustment of conduction syringe location and Faradaic current adjustment: open DC power supply unit 5, and according to the biomass cells designed in advance the three-dimensional profiles fixed, adjust by the position of described needle tubing travel mechanism to described conduction needle tubing and/or the current value of DC power supply unit 5 is adjusted, just obtain the three-dimensional space fixed sturcture of biomass cells; Described three-dimensional space fixed sturcture is be positioned on conductive soleplate 4 and the hydrogel 6 of embedded biometric cell.
Thus, when adopting cell three-dimensional apparatus for assembling as shown in Figure 1 to carry out cell three-dimensional assembling, cell stationary liquid electrolyzer anode generation electrolysis and generate hydrogel 6.Adjust the position of described conduction needle tubing and/or the Faradaic current value size by adjustment DC power supply unit 5 by described needle tubing travel mechanism, the shape by the hydrogel 6 on described conductive soleplate 4 is adjusted.
During actual use, move by mechanical arm 1 position that described conduction needle tubing just can change electric field, thus change Ca
2+the position generated; Controlled the size of electric current by DC power supply unit 5, namely control the intensity of electric field, thus control Ca
2+the concentration generated, so just can control effectively to the shape of the hydrogel containing cell, realize at three-dimensional cell assembling.Herein, the reaction of the generation alginate calcium occurred by the anode of apparatus for electrolyzing becomes " electrodeposit reaction ".
The above; it is only preferred embodiment of the present invention; not the present invention is imposed any restrictions, every above embodiment is done according to the technology of the present invention essence any simple modification, change and equivalent structure change, all still belong in the protection domain of technical solution of the present invention.
Claims (10)
1. the cell three-dimensional apparatus for assembling based on travelling electrode, it is characterized in that: comprise conductive soleplate (4), be positioned at the conduction needle tubing of conductive soleplate (4) top, the cell stationary liquid of embedded biometric cell be delivered to the priming device of described conduction needle tubing and drive described conduction needle tubing in the needle tubing travel mechanism of conductive soleplate (4) top movement, described conduction needle tubing is arranged in described needle tubing travel mechanism; Described conduction needle tubing comprises inner sleeve (3) and covers the conductive metal layer (3-1) on inner sleeve (3) outer side wall, the upper end of described inner sleeve (3) has fluid inlet and its lower end has liquid outlet, and described priming device is connected by the fluid inlet of pipe connecting with inner sleeve (3); Described cell stationary liquid is by the aqueous solution of sodium alginate, calcium carbonate and water Homogeneous phase mixing, and in the described aqueous solution, the weight percentage of sodium alginate is 1% ~ 4%, and in the described aqueous solution, the weight percentage of calcium carbonate is 0.1% ~ 0.4%; Described conductive soleplate (4) and conductive metal layer (3-1) connect with two power output ends of DC power supply unit (5) respectively, described conductive soleplate (4), conductive metal layer (3-1) and DC power supply unit (5) composition carries out electrolysis to described cell stationary liquid and generates the electrolyzer of hydrogel (6), and described conductive soleplate (4) and conductive metal layer (3-1) are electrode for electrolysis; Described conduction needle tubing is the coating unit be coated in by the hydrogel (6) of the cell stationary liquid of embedded biometric cell or embedded biometric cell on conductive soleplate (4).
2. according to a kind of cell three-dimensional apparatus for assembling based on travelling electrode according to claim 1, it is characterized in that: described DC power supply unit is the adjustable adjustable DC power supply of current value, and described priming device and/or described pipe connecting are equipped with the flow regulation device regulated the cell stationary liquid flow of the embedded biometric cell in described pipe connecting.
3. according to a kind of cell three-dimensional apparatus for assembling based on travelling electrode described in claim 1 or 2, it is characterized in that: described conductive soleplate (4) is laid in level, described conduction needle tubing is vertically to laying.
4. according to a kind of cell three-dimensional apparatus for assembling based on travelling electrode described in claim 1 or 2, it is characterized in that: described priming device is syringe (2), described inner sleeve (3) is glass needle tubing.
5. according to a kind of cell three-dimensional apparatus for assembling based on travelling electrode described in claim 1 or 2, it is characterized in that: the middle and upper part of described inner sleeve (3) is cylindrical tube and its underpart is diameter diminishing conical tube from top to bottom, and the upper end diameter of described conical tube is identical with the diameter of described cylindrical tube and its bottom is described liquid outlet; The bore of described liquid outlet is Φ 50 μm ~ Φ 200 μm.
6. according to a kind of cell three-dimensional apparatus for assembling based on travelling electrode described in claim 1 or 2, it is characterized in that: described needle tubing travel mechanism is mechanical arm (1), described mechanical arm (1) comprises base, the column be arranged on described base, be fixed on the sway brace on described column and be arranged on the clamping device clamped on described sway brace and to described conduction needle tubing top.
7. according to a kind of cell three-dimensional apparatus for assembling based on travelling electrode described in claim 1 or 2, it is characterized in that: the middle and lower part outer side wall of described inner sleeve (3) is all coated with conductive metal layer (3-1), described conductive metal layer (3-1) is metal deposition layer.
8., according to a kind of cell three-dimensional apparatus for assembling based on travelling electrode according to claim 7, it is characterized in that: described metal deposition layer is deposition of gold layer.
9. according to a kind of cell three-dimensional apparatus for assembling based on travelling electrode described in claim 1 or 2, it is characterized in that: positive supply output terminal or the negative supply output terminal of described conductive metal layer (3-1) and DC power supply unit (5) are connected; When the positive supply output terminal of conductive metal layer (3-1) and DC power supply unit (5), described conductive soleplate (4) is connected with the negative supply output terminal of current controller (5), now conductive metal layer (3-1) anode that is described electrolyzer, and described conduction needle tubing is the coating unit be coated in by the hydrogel of embedded biometric cell (6) on conductive soleplate (4); When the negative supply output terminal of conductive metal layer (3-1) and DC power supply unit (5), described conductive soleplate (4) is connected with the positive supply output terminal of current controller (5), now conductive soleplate (4) anode that is described electrolyzer, and described conduction needle tubing is the coating unit be coated in by the cell stationary liquid of embedded biometric cell on conductive soleplate (4).
10. utilize cell three-dimensional apparatus for assembling as claimed in claim 1 to carry out a method for cell three-dimensional assembling, it is characterized in that the method comprises the following steps:
Step one, electrolyzer wiring: by conductive soleplate (4) and conductive metal layer (3-1), connect with two power output ends of DC power supply unit (5) respectively;
Step 2, cell stationary liquid are carried: after biomass cells being put into described biological fixation liquid, send in described conduction needle tubing by priming device by the biological fixation liquid of embedded biometric cell;
Step 3, the adjustment of conduction syringe location and Faradaic current adjustment: open DC power supply unit (5), and according to the biomass cells designed in advance the three-dimensional profiles fixed, adjust by the position of described needle tubing travel mechanism to described conduction needle tubing and/or the current value of DC power supply unit (5) is adjusted, just obtain the three-dimensional space fixed sturcture of biomass cells; Described three-dimensional space fixed sturcture is gone up and the hydrogel (6) of embedded biometric cell for being positioned at conductive soleplate (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510082367.7A CN104593257A (en) | 2015-02-15 | 2015-02-15 | Three-dimensional cell assembly device and method based on movable electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510082367.7A CN104593257A (en) | 2015-02-15 | 2015-02-15 | Three-dimensional cell assembly device and method based on movable electrode |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104593257A true CN104593257A (en) | 2015-05-06 |
Family
ID=53119316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510082367.7A Pending CN104593257A (en) | 2015-02-15 | 2015-02-15 | Three-dimensional cell assembly device and method based on movable electrode |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104593257A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105616005A (en) * | 2015-12-31 | 2016-06-01 | 北京理工大学 | Device and method for assembling artificial microtissues based on machine-human cooperated operation |
| CN109971613A (en) * | 2019-04-03 | 2019-07-05 | 重庆工商大学 | A kind of device of guidance magnetic induction intensity lattice-like distribution |
| WO2019219380A1 (en) * | 2018-05-16 | 2019-11-21 | Robert Bosch Gmbh | Device for the electrochemical processing of components of a battery cell, method for operating same and use thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102209779A (en) * | 2008-09-16 | 2011-10-05 | 马克思-普朗克科学促进协会 | Electrode device, generator device and method for power generation by means of membrane-potential shunting |
| CN102258481A (en) * | 2011-08-19 | 2011-11-30 | 薛巍 | Method for preparing self-assembled medicine-carried microspheres by combining high-voltage electrostatic liquid droplet method and layer-by-layer self-assembly method |
-
2015
- 2015-02-15 CN CN201510082367.7A patent/CN104593257A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102209779A (en) * | 2008-09-16 | 2011-10-05 | 马克思-普朗克科学促进协会 | Electrode device, generator device and method for power generation by means of membrane-potential shunting |
| CN102258481A (en) * | 2011-08-19 | 2011-11-30 | 薛巍 | Method for preparing self-assembled medicine-carried microspheres by combining high-voltage electrostatic liquid droplet method and layer-by-layer self-assembly method |
Non-Patent Citations (3)
| Title |
|---|
| YAJING SHEN ET AL.: "3D Cell Assembly based on Electro Deposition of Calcium Alginate", 《MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS), 2012 INTERNATIONAL SYMPOSIUM ON》, 31 December 2012 (2012-12-31) * |
| 孙艳玲等: "海藻酸钙水凝胶纤维的制备及应用", 《纺织科学研究》, no. 4, 30 April 2014 (2014-04-30), pages 108 - 110 * |
| 赵名艳等: "可注射海藻酸钙水凝胶的制备研究", 《功能材料》, vol. 41, no. 8, 31 December 2010 (2010-12-31), pages 1353 - 1356 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105616005A (en) * | 2015-12-31 | 2016-06-01 | 北京理工大学 | Device and method for assembling artificial microtissues based on machine-human cooperated operation |
| WO2019219380A1 (en) * | 2018-05-16 | 2019-11-21 | Robert Bosch Gmbh | Device for the electrochemical processing of components of a battery cell, method for operating same and use thereof |
| CN109971613A (en) * | 2019-04-03 | 2019-07-05 | 重庆工商大学 | A kind of device of guidance magnetic induction intensity lattice-like distribution |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104593257A (en) | Three-dimensional cell assembly device and method based on movable electrode | |
| CN106044970A (en) | Method for flow-electrode capacitive deionization (FCDI)-based desalination and application | |
| Nikolić et al. | Correlate between morphology of powder particles obtained by the different regimes of electrolysis and the quantity of evolved hydrogen | |
| CN102431964A (en) | Method for controllable generation of quantum dots or quantum wires | |
| CN110205657A (en) | A kind of electrolytic copper foil plate electroplating experiments device | |
| CN204039531U (en) | A kind of electrophoretic deposition set on conductive surface | |
| CN104313657A (en) | Electro-deposition device of through hole of HDI printed circuit board | |
| CN107400913A (en) | Electrochemical metal needle point 3D printer and Method of printing | |
| CN103710735B (en) | A kind of electrochemical surface treatment method and device of porous metal material | |
| CN108588803A (en) | A kind of electric deposition device | |
| CN107881535A (en) | A kind of device and process for preparing metal base ultra-hydrophobicity surface | |
| TW431898B (en) | Energetic healer-a method and apparatus for improving the well-being of a human body | |
| CN104988564A (en) | Multi-functional intelligent plating bath | |
| CN208829786U (en) | A kind of electroplanting device | |
| CN102533547B (en) | Cell culture device with shear force-uniform electric field stimulation | |
| CN102020341A (en) | Reductive water non-diaphragm electrolysis device | |
| CN203618574U (en) | Fish behavior-and-nerve observing and researching device | |
| CN109468668A (en) | A kind of preparation method and applications of platinum nano-cone array structure | |
| CN108536197A (en) | A kind of porous air inlet Markov bottle and application method | |
| CN204714911U (en) | New device prepared by nano cerium dioxide powder | |
| CN108018597A (en) | A kind of novel electroplating hanger | |
| CN202626340U (en) | Partial electroplate equipment for column-shaped part | |
| CN106757293B (en) | A kind of preparation facilities of structural type infiltration gradient surface | |
| CN204097586U (en) | Multifunctional electrolysis slot device | |
| CN108796586B (en) | A kind of 3D printing method of the engraved structure based on light orientation electrophoretic deposition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150506 |
|
| RJ01 | Rejection of invention patent application after publication |