CN104586937A - Preparation method of illicium plants injection - Google Patents
Preparation method of illicium plants injection Download PDFInfo
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- CN104586937A CN104586937A CN201310531489.0A CN201310531489A CN104586937A CN 104586937 A CN104586937 A CN 104586937A CN 201310531489 A CN201310531489 A CN 201310531489A CN 104586937 A CN104586937 A CN 104586937A
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Abstract
The invention discloses a preparation method of an illicium plants injection, which is characterized in that root bark or stem bark of the illicium plants is percolated or immersed by ethanol with concentration of 50-95% to obtain a soup, then the soup is filtered, adjusting PH value of a filtrate, and recovering ethanol to prepare the illicium plants injection. The prepared injection has the efficacy of diminishing swelling, dissipating blood stasis, invigorating blood circulation, and relieving pain, can be used for treating lumbar muscle strain, joint or muscle ligament pain and rheumatalgia.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to utilize a kind of Illicium plant to prepare injection.
Background technology
Illicium plant is Magnoliaceae Illicium, also have people by single-row for Illicium for Winteraceae Illicium, this platymiscium comprises Radix Illicii Lanceolati (Illicium henryi), Illicium jiadifengpi (Illicium angustisepalum), Cortex Illicii (Illicium difengpi), lanceolata anise (formal name used at school: Illicium lanceolatum), large anistree (Illicium majus), Fructus Anisi Stellati (Illicium verum) etc.The root bark of this platymiscium and peel of stem have the effects such as detumescence dissipating blood stasis, promoting blood circulation and stopping pain, antiinflammatory, analgesia.May be used for traumatic injury, the arthritic treatment of rheumatic and rheumatoid.This constituents is made injection, and can directly act on focus, curative effect is rapider.
Summary of the invention
The object of this invention is to provide the medicine that a kind of onset is rapid, directly act on the disease such as treatment traumatic injury, rheumatic and rheumatoid arthritis of focus.
The object of the invention is to realize in the following manner:
A preparation method for Illicium plant injection, is characterized in that the method comprises the following steps: by the root bark of Illicium plant or peel of stem 50-95% ethanol percolation or immersion, the medical filtration obtained, and filtrate regulates or do not regulate pH value, reclaim ethanol, cold preservation, gets supernatant, filter, filtrate adds water and stabilizing agent dilution, cold preservation, filter, filtrate adjusts PH to 3-8, cold preservation, filter, filtrate adds surfactant and water, adjusts PH to 5-10, filter, embedding, obtained Illicium plant injection.
Detailed description of the invention
Below by specific embodiment, the present invention is further described, it should be pointed out that under the premise without departing from the principles of the invention, can also make some modification and improvement, and these also should be considered as belonging to protection scope of the present invention.
Embodiment 1
Radix Illicii Lanceolati (Illicium henryi) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 48 hours that concentration is 80%, percolation, collect the first liquid of filtering of medical material 4 times amount, separately deposit, continue the continuous liquid of filtering of collection 4 times amount, decompression recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 24 hours, get supernatant to filter, inject and use water 400ml, add stabilizing agent, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.6, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 2
Lanceolata anise (formal name used at school: Illicium lanceolatum) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 24 hours that concentration is 70%, percolation, collect the first liquid of filtering of medical material 4 times amount, separately deposit, continue the continuous liquid of filtering of collection 4 times amount, percolate merges, filter, filtrate adjusts PH to 10.2, decompression recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add stabilizing agent, stir evenly, by hydrochloric acid solution adjust ph to about 6.0, refrigerated overnight, filter, filtrate adds surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.6, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 3
Illicium jiadifengpi (Illicium angustisepalum) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, filtrate adjusts PH to 8.6, decompression recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 4
Cortex Illicii (Illicium difengpi) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 5
Large anise (Illicium majus) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 6
Fructus Anisi Stellati (Illicium verum) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 7
Radix Illicii Lanceolati (Illicium henryi) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 80%, filter, filtrate regulates pH value to 8-10, leave standstill 12-72 hour, filter, filtrate regulates pH value to 6-8, filter, filtrate recycling ethanol is to about 50-100ml, cold preservation 24-72 hour, get supernatant to filter, inject and use water 400ml, stir evenly, refrigerated overnight, filter, filtrate adds surfactant, add stabilizing agent, stir evenly, inject with water appropriate, adjust ph is to about 8.6, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 8
Lanceolata anise (formal name used at school: Illicium lanceolatum) root bark or peel of stem 50g, be processed into fritter or coarse powder, add concentration be 75% soak with ethanol soak 7 ~ 10 days, filter, filtrate adjusts PH to 9.8, decompression recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add stabilizing agent, stir evenly, by hydrochloric acid solution adjust ph to about 6.0, refrigerated overnight, filter, filtrate adds surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.6, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 9
Illicium jiadifengpi (Illicium angustisepalum) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, filtrate adjusts PH to 8.6, decompression recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 10
Cortex Illicii (Illicium difengpi) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 11
Large anise (Illicium majus) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 12
Fructus Anisi Stellati (Illicium verum) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 13
Radix Illicii Lanceolati (Illicium henryi) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 80%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 24 hours, get supernatant to filter, inject and use water 400ml, add stabilizing agent, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.6, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 14
Lanceolata anise (formal name used at school: Illicium lanceolatum) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 80%, filter, filtrate regulates pH value to 8-10, leave standstill 12-72 hour, filter, filtrate regulates pH value to 6-8, filter, filtrate recycling ethanol is to about 50-100ml, cold preservation 24-72 hour, get supernatant to filter, inject and use water 400ml, stir evenly, refrigerated overnight, filter, filtrate adds surfactant, add stabilizing agent, stir evenly, inject with water appropriate, adjust ph is to about 8.6, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 15
Illicium jiadifengpi (Illicium angustisepalum) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, filtrate adjusts PH to 8.6, decompression recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 16
Cortex Illicii (Illicium difengpi) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 17
Large anise (Illicium majus) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Embodiment 18
Fructus Anisi Stellati (Illicium verum) root bark or peel of stem 50g, be processed into fritter or coarse powder, add the soak with ethanol 7 ~ 10 days that concentration is 70%, filter, decompression filtrate recycling ethanol, leave standstill 24 hours, filter, filtrate recycling ethanol is extremely without alcohol taste, cold preservation 48 hours, get supernatant to filter, inject and use water 400ml, add 1g sodium pyrosulfite, stir evenly, by hydrochloric acid solution adjust ph to about 7.0, refrigerated overnight, filter, filtrate adds 20ml surfactant, stir evenly, inject with water appropriate, by 10% sodium hydroxide adjust ph to about 8.0, inject and make into 1000ml with water, stir evenly, filter, embedding, sterilizing, obtain.
Claims (6)
1. a preparation method for Illicium plant injection, is characterized in that the method comprises the following steps: by the root bark of Illicium plant or peel of stem 50-95% ethanol percolation or immersion, the medical filtration obtained, and filtrate regulates or do not regulate pH value, reclaim ethanol, cold preservation, gets supernatant, filter, filtrate adds water and stabilizing agent dilution, cold preservation, filter, filtrate adjusts PH to 3-8, cold preservation, filter, filtrate adds surfactant and water, adjusts PH to 5-10, filter, embedding, obtained Illicium plant injection.
2. the preparation method according to claims 1, is characterized in that Illicium plant comprises: Radix Illicii Lanceolati (Illicium henryi), Illicium jiadifengpi (Illicium angustisepalum), Cortex Illicii (Illicium difengpi), lanceolata anise (formal name used at school: Illicium lanceolatum), large anistree (Illicium majus), Fructus Anisi Stellati (Illicium verum) etc.
3. the preparation method according to claims 1, is characterized in that the root bark of Illicium plant or peel of stem 50-95% ethanol percolation or immersion.
4. the preparation method according to claims 1, it is characterized in that percolation or soaking medicated liquid to filter, filtrate regulates or does not regulate pH value, reclaim ethanol, cold preservation, gets supernatant, filter, filtrate adds water and stabilizing agent dilution, cold preservation, filter, filtrate adjusts PH to 3-8, cold preservation, filter, filtrate adds surfactant and water, adjusts PH to 5-10, filter, embedding, obtained Illicium plant injection.
5. the preparation method according to claims 1, is characterized in that stabilizing agent comprises sodium sulfite, sodium pyrosulfite, disodiumedetate, citric acid (CA), tartaric acid (TA) and gluconic acid (GA) etc.
6. the preparation method according to claims 1, it is characterized in that surfactant comprises: arabic gum, stearic acid, dodecylbenzene sodium sulfonate, quaternary ammonium compound, lecithin, aminoacid, betanin, fatty glyceride, fatty acid Pyrusussuriensis smooth (span), Polysorbate.
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Cited By (2)
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| CN105816524A (en) * | 2016-04-18 | 2016-08-03 | 广西壮族自治区中国科学院广西植物研究所 | Anti-inflammatory active cortex illicii extract as well as preparation method and application thereof |
| CN106109581A (en) * | 2016-06-30 | 2016-11-16 | 浙江泰康药业集团有限公司 | Injection liquid of red fennel ultrafiltration technology |
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Application publication date: 20150506 |