CN102895311A - Preparation method of illicium henryi injecta - Google Patents
Preparation method of illicium henryi injecta Download PDFInfo
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- CN102895311A CN102895311A CN201210458686XA CN201210458686A CN102895311A CN 102895311 A CN102895311 A CN 102895311A CN 201210458686X A CN201210458686X A CN 201210458686XA CN 201210458686 A CN201210458686 A CN 201210458686A CN 102895311 A CN102895311 A CN 102895311A
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Abstract
The invention discloses a preparation method of illicium henryi injecta, comprising the following steps of: collecting volatile oil extracting solution (solution A) from velamen or bark coarse powder of illicium henryi by a steam distillation method to be stored; filtering the residual liquid medicine after distilling, and concentrating to be taken as a concentrated water solution (solution B) to be stored; and carrying out reflux extraction on the dregs of a decoction with ethanol to obtain ethanol extract solution (solution C), and merging the solution A, the solution B and the solution C to prepare the injecta. The injecta prepared by the method disclosed by the invention has the effects of removing tumescence, dissipating stasis, and promoting blood circulation to arrest pain, and can be used for treating the lumbar muscle strain, the joint or muscle and ligament pain, the rheumatalgia and the like.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to a kind of preparation method of Injection liquid of red fennel.
Background technology
The root that Radix Illicii Lanceolati chain timbers the orchid family Illicium plant Folium illicii Lanceolati (Lllicium lanceolatum A.C Smith) is dry or the skin of stem have the effect of detumescence dissipating blood stasis, promoting blood circulation and stopping pain, are used for traumatic injury, the treatment of rheumatism atrophic arthritis.Except idol among the people powder is arranged at present, outside the oral or above-mentioned disease of external curing of medicated wine, existing Zhejiang Tai Kang Pharmaceutical Group Co.,Ltd produces Injection liquid of red fennel without competition, former technique is ethanol extract from water precipitation, exist effective site to use incomplete problem (particularly volatile oil part), therefore the preparation technology of this Injection liquid of red fennel needs improvement badly.
Summary of the invention
The objective of the invention is to overcome the preparation method that above-mentioned weak point provides a kind of Injection liquid of red fennel.
The objective of the invention is to realize in the following manner:
A kind of preparation method of Injection liquid of red fennel, the method may further comprise the steps: adopt the vapor distillation extraction to obtain the volatile oil extracting solution root bark or the peel of stem coarse powder of Radix Illicii Lanceolati, other deposits; Filter the residue medicinal liquid, be concentrated into 1 ~ 2 times of amount of medical material, be concentrated aqueous liquid, other deposits; Be that 90% ~ 95% alcohol heating reflux extracts and to obtain alcohol extract with the medicinal residues concentration that filters out, volatile oil extracting solution, water extraction liquid, alcohol extract three parts are merged, make injection.
It is as follows that described vapor distillation extracts the step that obtains the volatile oil extracting solution: the water soaking of bark of Radix Illicii Lanceolati or 10 ~ 15 times of amounts of peel of stem coarse powder usefulness medical material 4 ~ 8 hours, vapor distillation extracts volatile oil, collects the volatile oil extracting solution of 5 ~ 6 times of amounts of medical material.
Above-mentioned alcohol heating reflux extracts and is specially medicinal residues concentration is that 90% ~ 95% alcohol heating reflux extracts 2 ~ 3 times, and the concentration of at every turn using 2 ~ 3 times of amounts of medical material is 90% ~ 95% ethanol, each 1 hour extraction time, merges ethanol extract.
Described volatile oil extracting solution, water extraction liquid, alcohol extract three parts merge the step of making injection: add concentrated aqueous liquid in ethanol extract, stir, leave standstill cold preservation more than 24 hours, filter, filtrate recycling ethanol is to obtaining concentrated solution without the alcohol flavor, and the concentration that adds again 2 ~ 3 times of amounts of concentrated solution is 95% ethanol, staticly settles, filter, filtrate recycling ethanol is to distinguishing the flavor of without alcohol, and the medicinal liquid behind the Recycled ethanol adds the water for injection of 4 ~ 6 times of amounts of medical material, and adds activated carbon decolorizing, cold preservation is more than 24 hours, filter, filtrate is transferred pH to 7.0 ~ 7.5, adds the volatile oil extracting solution, get oil-water mixture, with the tween 80 stirring and dissolving of mixed liquor weight 2%, leave standstill cold preservation more than 24 hours again, filter, transfer again pH to 9.7, add to the full amount of water for injection agitation and filtration, embedding, sterilization, and get final product.Employing concentration is 10% NaOH solution adjust pH in the said method.
Beneficial effect of the present invention compared with the prior art: preparation method of the present invention is extracted all the other effective sites with water extraction-alcohol extraction combined techniques again for using first the extraction by steam distillation volatile oil part, adds volatile oil part and makes injection.The inventive method takes full advantage of each effective site of medical material, and the injection for preparing has the detumescence dissipating blood stasis, and the effect of promoting blood circulation and stopping pain is used for the treatment of lumbar muscle strain, and joint or the muscle ligament pain of injury and rheumatalgia etc. have significant curative effect and safety, curative effect is lasting.
The specific embodiment
The present invention is further described below by specific embodiment; should be pointed out that for the person of ordinary skill of the art, under the prerequisite that does not break away from the principle of the invention; can also make some modification and improvement, these also should be considered as belonging to protection scope of the present invention.
Embodiment 1
Radix Illicii Lanceolati peel of stem 50g is processed into coarse powder, adds the water soaking 8 hours of 10 times of amounts of medical material, and vapor distillation is collected volatile oil extracting solution 300mL, and other deposits; With the residue medical filtration behind the volatile matter distillation oil, being concentrated into 50mL(is 1 times of amount of medical material) be concentrated aqueous liquid, other deposits.Medicinal residues concentration after the filtration is that 95% alcohol heating reflux extracts 2 times, each ethanol consumption is 100mL, each 1 hour extraction time merges ethanol extract, adds concentrated aqueous liquid and stirs, left standstill cold preservation 24 hours, filter, filtrate recycling ethanol is to obtaining concentrated solution without the alcohol flavor, adds 2 times of amounts of concentrated solution concentration again and be 95% ethanol precipitation more than 24 hours, filter, filtrate recycling ethanol is to distinguishing the flavor of without alcohol, and the medicinal liquid behind the Recycled ethanol injects water 300mL, and adds the 5g activated carbon decolorizing, cold preservation, filter, filtrate is 10% NaOH solution accent pH to 7.0 ~ 7.5 with concentration, adds aforementioned volatile oil extracting solution, get oil-water mixture, with the tween 80 stirring and dissolving of mixed liquor weight 2%, left standstill cold preservation 24 hours, filter, be that 10% NaOH solution is transferred pH to 9.7 with concentration again, inject water to 1000mL, agitation and filtration, embedding, sterilization, and get final product.
Embodiment 2
Radix Illicii Lanceolati peel of stem 50g is processed into coarse powder, adds the water soaking 4 hours of 15 times of amounts of medical material, and vapor distillation is collected volatile oil extracting solution 300mL, and other deposits.Residue medical filtration behind the volatile matter distillation oil, being concentrated into 100mL(is 2 times of amounts of medical material) being concentrated aqueous liquid, other deposits.Medicinal residues concentration after the filtration is that 90% alcohol heating reflux extracts 3 times, each ethanol consumption is 150mL, each 1 hour extraction time, merge ethanol extract, add concentrated aqueous liquid and stir, leave standstill cold preservation more than 24 hours, filter, filtrate recycling ethanol is to obtaining concentrated solution without alcohol flavor, adds 3 times of amounts of concentrated solution concentration again and be 95% ethanol precipitation more than 24 hours, filters, filtrate recycling ethanol is to distinguishing the flavor of without alcohol, medicinal liquid behind the Recycled ethanol injects water 300mL, and adds the 8g activated carbon decolorizing, and cold preservation is filtered, filtrate is that 10%NaOH solution is transferred pH to 7.0 ~ 7.5 with concentration, add aforementioned volatile oil extracting solution, get oil-water mixture, with the tween 80 stirring and dissolving of mixed liquor weight 2%, left standstill cold preservation 24 hours, filtering, is that 10%NaOH solution is transferred pH to 9.7 with concentration again, injects water to 1000mL, agitation and filtration, embedding, sterilization, and get final product.
Below further specify the effect of injection of the present invention by effect experiment:
Test example 1
Adopt mouse tail to press the analgesic and anti-inflammatory effects of analgesic experiment, mice ear experiment and rat paw edema laboratory observation Radix Illicii Lanceolati peel of stem volatile oil extract part (HHX-1), water extract-alcohol precipitation position (HHX-2), the embodiment of the invention 1 group of (Injection liquid of red fennel of the inventive method preparation), the embodiment of the invention 2 groups of (Injection liquid of red fennel of the inventive method preparation), Comparative Examples groups (Injection liquid of red fennel that commercially available Zhejiang Tai Kang Pharmaceutical Group Co.,Ltd produces).
Radix Illicii Lanceolati peel of stem volatile oil extract part (HHX-1): get Radix Illicii Lanceolati peel of stem 50g and be processed into coarse powder, add the water soaking 8 hours of 10 times of amounts of medical material, vapor distillation is collected volatile oil extracting solution 300mL.
Radix Illicii Lanceolati peel of stem water extract-alcohol precipitation position (HHX-2): will be according to the residue medical filtration behind the embodiment 1 method volatile matter distillation oil, being concentrated into 50mL is concentrated aqueous liquid, other deposits.Medicinal residues concentration after the filtration is that 95% alcohol heating reflux extracts 2 times, and each ethanol consumption is 100mL, each 1 hour extraction time, merge ethanol extract, add concentrated aqueous liquid and stir, left standstill cold preservation 24 hours, filter, filtrate recycling ethanol is to obtaining concentrated solution without the alcohol flavor, add 2 times of amounts of concentrated solution dehydrated alcohol precipitation more than 24 hours again, filter, filtrate recycling ethanol is to nothing alcohol flavor, add water to 300mL, and get final product.
1 impact on mouse tail tenderness pain threshold
Choose the SPF level KM mice of body weight 18 ~ 22g, mice is placed in the holder, afterbody and extremity are exposed to outside the holder, test after the animal peace and quiet.Survey before the pain at mouse tail apart from tail point 1/3 place as the compression point labelling, occur struggling or shouting as the pain reaction index with mice when exerting pressure, this force value is this Mus pain threshold, tie-in secondary, the in contrast value of averaging.Select altogether 80 mices, be divided at random 8 groups (10/group), male and female half and half, namely blank group, YUNNAN BAIYAO group (5mL/kg), diclofenac group (50mg/kg), HHX-1 group (500mg/kg), HHX-2 organize (500mg/kg), the embodiment of the invention 1 group of (500mg/kg), the embodiment of the invention 2 groups of (500mg/kg), Comparative Examples groups (500mg/kg).With microsyringe medicine is applied in the mouse tail pressure spot during administration, 5mL/kg, 15min, 30min pain threshold after the mensuration administration calculate the Δ threshold of pain (the front pain threshold of pain threshold-medicine behind the Δ threshold of pain=medicine).The result shows: HHX-2 group 15min after administration can significantly improve mouse tail Pain-threshold (P＜0.05), and the HHX-1 group has no remarkable increase mouse tail Pain-threshold; Injection liquid of red fennel and Comparative Examples group Injection liquid of red fennel 15min after administration of the inventive method preparation all can significantly improve mouse tail Pain-threshold (P＜0.05), but the Injection liquid of red fennel analgesic effect of the inventive method preparation is better, and the present invention and Comparative Examples group relatively have very significant difference, specifically see Table 1-1.
Table 1-1 is on the impact of mice tenderness pain threshold
Annotate: compare * P＜0.05 with the blank group.
Compare △ P＜0.05 with the Comparative Examples group.
2 impacts on mice ear
Get SPF level male KM mice, body weight 18 ~ 22g, be divided at random 8 groups (10/group), namely blank group, YUNNAN BAIYAO group (5mL/kg), diclofenac group (50mg/kg), HHX-1 group (500mg/kg), HHX-2 organize (500mg/kg), the embodiment of the invention 1 group of (500mg/kg), the embodiment of the invention 2 groups of (500mg/kg), Comparative Examples groups (500mg/kg).With microsyringe medicine is applied in mice left and right sides auricle, 5mL/kg during administration.Behind the auricle administration 30min, utilize microsyringe that 0.05mL dimethylbenzene is dripped respectively in the auris dextra both sides, guarantee drip position consistency, left ear compares.Behind the 3h mice is taken off cervical vertebra and put to death, cut two ears along the auricle baseline, lay circular auricle at the same position of left and right ear respectively with the card punch of diameter 9mm, weigh, ask the difference of left and right sides auricle weight as swelling, compare group difference with the blank group.The result shows: compare with the blank group, HHX-1 and HHX-2 group all can alleviate dimethylbenzene induced mice ear thickness (P＜0.05); The Injection liquid of red fennel of the inventive method preparation can extremely significantly alleviate dimethylbenzene induced mice ear thickness (P＜0.01), and the present invention and Comparative Examples group relatively have very significant difference, more remarkable than Comparative Examples group Injection liquid of red fennel antiphlogistic effects, specifically see Table 1-2.
Table 1-2 is on the impact of mice ear
Annotate: compare * P＜0.05, * * P＜0.01 with the blank group.
Compare △ P＜0.05 with the Comparative Examples group.
3 impacts on the rat carrageenan pedal swelling
Get SPF level male rat, body weight 180 ~ 220g, be divided at random 8 groups (10/group), namely blank group, YUNNAN BAIYAO group (1mL/kg), diclofenac group (10mg/kg), HHX-1 group (100mg/kg), HHX-2 organize (100mg/kg), the embodiment of the invention 1 group of (100mg/kg), the embodiment of the invention 2 groups of (100mg/kg), Comparative Examples groups (100mg/kg).In right back ankle joint labelling, utilize sufficient sole of the foot capacity measurer to survey first the normal right back sufficient sole of the foot volume of rat.Utilize microsyringe that medicine is applied in right back sufficient sole of the foot section, 1mL/kg.Behind the toes administration 30min, every right back sufficient plantar subcutaneous injection 1% carrageenin 0.1mL of Mus, and after giving carrageenin 30,60,120,240,360min surveys Rat Right metapedes sole of the foot volume (mL) again, and calculate swelling (swelling degree of the paw=to different time foot sole of the foot volume behind the carrageenin-to before the carrageenin with parapodum sole of the foot volume).The result shows: with the blank group relatively, HHX-1 organizes that 30min can significantly alleviate rat paw edema degree (P＜0.05) after administration, HHX-2 group rat paw edema degree has no obviously and alleviates; The Injection liquid of red fennel of the inventive method preparation can significantly alleviate rat paw edema degree (P＜0.05), and the present invention and Comparative Examples group relatively have very significant difference, and is more remarkable than Comparative Examples group Injection liquid of red fennel effect, specifically sees Table 1-3.
Table 1-3 is on the impact of rat carrageenan pedal swelling
Annotate: compare * P＜0.05 with the blank group.
Compare △ P＜0.05 with the Comparative Examples group.
The analgesic and anti-inflammatory effects result of study shows: HHX-1 and HHX-2 group all can alleviate dimethylbenzene induced mice ear thickness, and HHX-1 organizes that 30min can significantly alleviate the rat paw edema degree after administration, and the HHX-2 group has no obviously alleviating of rat paw edema degree; But HHX-2 group 15min after administration can significantly improve the mouse tail Pain-threshold.Results suggest Radix Illicii Lanceolati peel of stem volatile oil extract part is better than the water extract-alcohol precipitation position for the inhibitory action of inflammation, but the analgesic activity at water extract-alcohol precipitation position is better than the volatile oil extract part; The Injection liquid of red fennel that the Injection liquid of red fennel that adopts the inventive method preparation prepares than existing technique and HHX-1 and the antalgic and inflammation relieving effect potentiation of HHX-2 position are remarkable.
Claims (5)
1. the preparation method of an Injection liquid of red fennel is characterized in that the method may further comprise the steps: adopt vapor distillation to extract the root bark of Radix Illicii Lanceolati or peel of stem coarse powder and obtain the volatile oil extracting solution, other deposits; Filter the residue medicinal liquid, be concentrated into 1 ~ 2 times of amount of medical material, be concentrated aqueous liquid, other deposits; Be that 90% ~ 95% alcohol heating reflux extracts and to obtain alcohol extract with the medicinal residues concentration that filters out, volatile oil extracting solution, water extraction liquid, alcohol extract three parts are merged, make injection.
2. preparation method according to claim 1, it is characterized in that described vapor distillation extracts the step that obtains the volatile oil extracting solution as follows: the water soaking of bark of Radix Illicii Lanceolati or 10 ~ 15 times of amounts of peel of stem coarse powder usefulness medical material 4 ~ 8 hours, vapor distillation extracts volatile oil, collects the volatile oil extracting solution of 5 ~ 6 times of amounts of medical material.
3. preparation method according to claim 1, it is characterized in that described is that 90% ~ 95% alcohol heating reflux extracts 2 ~ 3 times with medicinal residues concentration, each concentration with 2 ~ 3 times of amounts of medical material is 90% ~ 95% ethanol, each 1 hour extraction time, merges ethanol extract.
4. preparation method according to claim 1, it is characterized in that described volatile oil extracting solution, water extraction liquid, alcohol extract three parts merge the step of making injection: add concentrated aqueous liquid in ethanol extract, stir, leave standstill cold preservation more than 24 hours, filter, filtrate recycling ethanol is to obtaining concentrated solution without the alcohol flavor, the concentration that adds again 2 ~ 3 times of amounts of concentrated solution is 95% ethanol, staticly settle, filter, filtrate recycling ethanol is to distinguishing the flavor of without alcohol, medicinal liquid behind the Recycled ethanol adds the water for injection of 4 ~ 6 times of amounts of medical material, and adding activated carbon decolorizing, cold preservation was filtered more than 24 hours, filtrate is transferred pH to 7.0 ~ 7.5, add the volatile oil extracting solution, get oil-water mixture, again with the tween 80 stirring and dissolving of mixed liquor weight 2%, leave standstill cold preservation more than 24 hours, filter, transfer again pH to 9.7, add to the full amount of water for injection, agitation and filtration, embedding, sterilization, and get final product.
5. preparation method according to claim 4 is characterized in that with concentration being 10% NaOH solution adjust pH.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104586937A (en) * | 2013-11-01 | 2015-05-06 | 浙江泰康药业集团有限公司 | Preparation method of illicium plants injection |
| CN105851068A (en) * | 2016-04-01 | 2016-08-17 | 江南大学 | Method for preparing antibacterial agent and bacterial quorum sensing inhibitor by using fennel |
| CN106265923A (en) * | 2015-06-07 | 2017-01-04 | 浙江泰康药业集团有限公司 | A kind of method improving clarity of injection |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634412A (en) * | 2004-10-29 | 2005-07-06 | 张平 | Lanceleaf anisetree freeze dried and preparation method thereof |
| CN102688294A (en) * | 2012-06-13 | 2012-09-26 | 浙江农林大学 | Illicium henryi-contained anti-virus ointment for external use and preparation method and application thereof |
-
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- 2012-11-14 CN CN201210458686.XA patent/CN102895311B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634412A (en) * | 2004-10-29 | 2005-07-06 | 张平 | Lanceleaf anisetree freeze dried and preparation method thereof |
| CN102688294A (en) * | 2012-06-13 | 2012-09-26 | 浙江农林大学 | Illicium henryi-contained anti-virus ointment for external use and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 陈烨丹等: "红茴香药学研究概况", 《安徽农业科学》, vol. 39, no. 14, 31 July 2011 (2011-07-31), pages 8376 - 8377 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104586937A (en) * | 2013-11-01 | 2015-05-06 | 浙江泰康药业集团有限公司 | Preparation method of illicium plants injection |
| CN106265923A (en) * | 2015-06-07 | 2017-01-04 | 浙江泰康药业集团有限公司 | A kind of method improving clarity of injection |
| CN105851068A (en) * | 2016-04-01 | 2016-08-17 | 江南大学 | Method for preparing antibacterial agent and bacterial quorum sensing inhibitor by using fennel |
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