CN102552860A - Chinese medicinal preparation for treating vertigo and preparation method thereof - Google Patents

Abstract

The invention discloses a Chinese medicinal preparation for treating vertigo and a preparation method thereof. The preparation method for the Chinese medicinal preparation comprises the following steps of: extracting gastrodia elata, uncaria, rhizoma alismatis, pinellia ternata, largehead atractylodes rhizome, tuckahoe, white paeony root, bamboo shaving, szechuan lovage rhizome, liquorice, orange peel, ginger and the like which serve as raw materials, and purifying to obtain extracts; and mixing the extracts and a pharmaceutical carrier serving as a substrate to obtain the Chinese medicinal preparation. The Chinese medicinal preparation has the effects of eliminating phlegm and treating the vertigo, and harmonizing stomach and preventing vomiting and has an obvious curative effect on the vertigo.

Description

Be used to Chinese medicine preparation of treating dizziness and preparation method thereof

Technical field

The present invention relates to a kind of Chinese medicine preparation, particularly a kind of Chinese medicine preparation that is used to treat dizziness and preparation method thereof.

Background technology

Dizzyly be shown in multiple disease; Quickening along with The development in society and economy and rhythm of life; The increase of the aggravation of social competition and people's psychology equal pressure etc.; Be obvious ascendant trend especially with the various dizzy morbidity of person in middle and old age's disease association, it should be noted that the dizzy of accidental or prolonged and repeated outbreak might be a signal of interest of serious disease.The traditional Chinese medical science is thought and is caused dizzy main diseases because stagnation of phlegm-damp in middle-JIAO, clear YANG failing to ascend, or disturb on the liver-yang, and stirring-up of pathogenic wind in the interior resulting from deficiency, or deficiency of kidney-essence, brain are lost and are supported, or deficiency of qi and blood, and it is foster etc. that brain is become homeless.And it is dizzy surely to eliminate the phlegm, and the stomach function regulating preventing or arresting vomiting is the dizzy method of treatment commonly used of clinical treatment.Dizzyly be most commonly in Meniere's syndrome, vertebral artery and basilar artery insufficiency, it is clinical mainly to show as dizzy, vomiting, light red tongue, white, slippery and moist fur; This performance is for hindering in the phlegm-damp heresy, cause clear YANG failing to ascend, turbid YIN failing to descend; Disturb on the wind-phlegm, stomach-QI being unable to descend normally, and its disease originally is to give birth to humidogene expectorant by spleen; Lure liver-wind, treatment is worked as with drying dampness to eliminate phlegm, suppressing the hyperactive liver to relieve the wind syndrome, stomach function regulating preventing or arresting vomiting.Rhizoma Pinelliae drying dampness to eliminate phlegm in the side, stopping nausea and vomiting by lowering the adverse flow of QI; The Rhizoma Gastrodiae suppressing the hyperactive liver to relieve the wind syndrome, end dizziness, both share, and are the key medicine of treatment dizziness due to wind-phlegm; Be equipped with the Rhizoma Atractylodis Macrocephalae, the spleen strengthening and damp drying, Ramulus Uncariae Cum Uncis, Radix Paeoniae Alba suppressing the hyperactive liver to relieve the wind syndrome, hold back sun, close principal agent with the spleen strengthening and damp drying, eliminate the phlegm dizzy surely; Assistant is with the Rhizoma Alismatis promoting diuresis to eliminate damp pathogen, with the Rhizoma Atractylodis Macrocephalae, Poria mutually 5, makes temper strong and dampness disappears, and dampness disappears and expectorant is not given birth to, with disconnected its source; Pericarpium Citri Reticulatae, Caulis Bambusae In Taenia, Rhizoma Zingiberis Recens activating QI to eliminate phlegm, stomach function regulating preventing or arresting vomiting make with the Rhizoma Chuanxiong promoting flow of QI and blood, dispel the wind, and priming is up; Radix Glycyrrhizae transfer medicine with in.This side's compatibility is the method for taste liver three through ruling together, spleen reinforcing stomach function regulating first, and activating QI to eliminate phlegm disappears the strong dampness of taste; Second suppressing the hyperactive liver is held back sun, is relieved dizziness, high fever, infantile convulsions, epilepsy, etc. only dizzyly, and then liver-wind is motionless.The refine of full side's compatibility, the spleen liver is ruled together, and has to eliminate the phlegm dizzyly surely, and the merit of stomach function regulating preventing or arresting vomiting is used to treat all kinds of dizzy due to dizzy, feels sick, vomiting, light red tongue, and cards such as white, slippery and moist fur especially close Ni Ershi disease finding person.Above-mentioned raw materials through extraction the Rhizoma Gastrodiae XUANYUNNING mixture [WS3-B-2661-97, the 14 of ministry standard] be prepared from, have eliminate the phlegm dizzy surely, the effect of stomach function regulating preventing or arresting vomiting.

As everyone knows, decoction and alcohol sedimentation technique makes classical impurity removal process, but the loss that the method is imitated composition to row is bigger, and the production cycle is long, and the remove impurity effect is limited.Therefore, we grope through a large amount of experiments on original basis, and under the prerequisite that guarantees drug effect, we have processed dosage forms such as tablet, hard capsule, soft capsule, drop pill, micropill by common process.

Summary of the invention

To the deficiency of existing product, the present invention adopts inclusion essential oil, and resin purification etc. are advanced, scientific and rational method for preparing, remove impurity as much as possible, keep effective ingredient to greatest extent.

The objective of the invention is to: Chinese medicine preparation of a kind of treatment dizziness evident in efficacy and preparation method thereof is provided.

The present invention realizes through following technical scheme: (1) raw material medicines in portions by weight consists of: 5 parts in Rhizoma Gastrodiae, 20 parts of 10 portions of Rhizoma Alismatis of Ramulus Uncariae Cum Uncis (system), 10 parts of Rhizoma Pinelliae (processed), 12 parts of the Rhizoma Atractylodis Macrocephalaes, 12 parts in Poria, 10 parts of the Radix Paeoniae Albas, 10 parts of Caulis Bambusae In Taenia, 3 parts of Rhizoma Chuanxiongs, 3 parts in Radix Glycyrrhizae (processing), 6 parts of Pericarpium Citri Reticulataes, Rhizoma Zingiberis Recens 4-5 part;

(2) get above-mentioned weight portion Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens, add 6-14 times of water, steam distillation extracted volatile oil 1-5 hour, and the volatile oil of collection slowly joins in the saturated solution that an amount of HP-β-CD processes, and it is subsequent use to be prepared into clathrate; Aqueous solution behind the extraction volatile oil is subsequent use; Get above-mentioned weight portion Rhizoma Gastrodiae, the Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Caulis Bambusae In Taenia and Pericarpium Citri Reticulatae, the merging of Rhizoma Zingiberis Recens medicinal residues, boil 2-3 time, each 1-2 hour, filter with 8-14 times of decocting; The aqueous solution of merging filtrate and above-mentioned Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens concentrates the back and goes up macroporous resin, and elder generation is with the water elution of 1-2 times of column volume; The ethanol elution of reuse 4-6 times of column volume 40-60% is collected alcoholic acid eluent, and concentrating under reduced pressure becomes extractum; Vacuum drying obtains extract, and is subsequent use;

(3) get above-mentioned weight portion Ramulus Uncariae Cum Uncis, Rhizoma Alismatis, the Rhizoma Pinelliae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Chuanxiong, with 50-100 times of 40-60% ethanol percolate extraction, percolate reclaims ethanol and concentrating under reduced pressure becomes extractum, and vacuum drying obtains extract, and is subsequent use;

(4) get the required axle material of above-mentioned clathrate and extract adding and can be made into oral formulations such as tablet, hard capsule, soft capsule, drop pill, micropill.

For confirming that the product that obtains has notable therapeutic effect, we have carried out a series of experiments:

Contrasting former Rhizoma Gastrodiae XUANYUNNING mixture with tablet of the present invention is example, main pharmacodynamics of the present invention is described as follows at present:

One, the influence test of the dizzy model nystagmus of rabbit due to the rotation of offset of sinusoidal ripple

2.5～3kg rabbit, each 30 of ♀ ♂ are divided into 5 groups: matched group ig distilled water, positive controls ig Rhizoma Gastrodiae XUANYUNNING mixture (10g raw medicinal herbs kg at random ^-1), the large, medium and small dosage of experimental group ig tablet of the present invention (5,10,20g raw medicinal herbs kg ^-1).Conscious rabbits is placed rabbit hutch; Cut off two paropias and the head part rabbit hair, expose skin, be positioned over electronic swivel chair; With two 0.5～1.0cm place Intradermal that insert rabbit right and left eyes outer canthus with the acupuncture needle self-made electrode respectively; Make leading electrode, the 3rd electrode insert head top Intradermal, electrode as a reference.The nystagmus signal is imported eight road physiology monitors through each electrode, keeps watch on through oscillograph, and traces the nystagmus waveform by recording stylus, muffles the rabbit head, leaves standstill several minutes, treats that baseline steadily starts swivel chair in the back, and peak angular velocity is 60 ° of S ^-1, frequency 0.25Hz, rotation 5min, waveform when recording start, 3min and 4min30s, each remembers 30s, observes after the stall and the record nystagmus persistent period.Physiograph condition: sensitivity 1mV/DIV, filtering 100Hz, chart drive speed 1cm/s, time constant 2s.1h rabbit nystagmus waveform before the record administration and after the administration, the following index of measurements and calculations: peak period slow phase angular velocity (each measuring point is respectively got the big ripple of 4 amplitudes, totally 12), crest frequency and nystagmus persistent period (after the stall).See table 1.

The influence of the dizzy model nystagmus of rabbit due to the rotation of table 1 offset of sinusoidal ripple

Annotate: with comparison before the administration ^*P＜005 ^*P＜001.

Visible by table 1, big or middle dose groups of the present invention and positive drug have obvious inhibitory action to slow phase angular velocity, and the nystagmus persistent period is obvious inhibitory action, and the two does not have obvious influence to average nystagmus frequency.

Two, the influence of Cavia porcellus toxemic vertigo reaction is tested

Get 50 of Cavia porcelluss, be divided into 5 groups at random: model group (normal saline), matched group (Rhizoma Gastrodiae XUANYUNNING mixture 10g raw medicinal herbs kg ¹) group, the large, medium and small dose groups of the present invention (5,10,20g raw medicinal herbs kg ¹).Administration every day 1 time, successive administration 14 days.1h only injects fast chloroform 20 μ L/ in 4～5mm place in Cavia porcellus external auditory meatus after the last administration, thereafter 20,40,60min examines, is observation index with tremble number of times, head tremor pendulum number of times of animal eyeball, and be 1min each observing time.See table 2.

The influence of table 2 pair Cavia porcellus toxemic vertigo reaction

Annotate: compare with model group, ^*P＜0.05, ^*P＜0.01

Table 2 can know that 3 dose groups of tablet of the present invention all can reduce Cavia porcellus toxemic vertigo different time head number of oscillations and nystagmus number of times (P＜0.05 or P＜0.01).

The influence of three, dizzy mice diving tower being tested

Get 60 of Kunming mouses, be divided into 6 groups at random: normal group (normal saline), model group, matched group (Rhizoma Gastrodiae XUANYUNNING mixture 10g raw medicinal herbs kg ¹), the large, medium and small dose groups of the present invention (5,10,20g raw medicinal herbs kg ¹) press 10mLkg ¹Gastric infusion, every day 1 time, successive administration 3 days.Experiment mice is escaped electric shock reflection training continuously in the diving tower appearance, training 3 times continuously is 1 time, and every day at the upper and lower noon respectively trains 1 time, penetrates to consolidate it, and 3d sets up stable and firm escape electric shock reflection continuously.1h administration before test after 3 days, train 1 time after, except that normal group; 50r/min on gyroscope at the uniform velocity rotates 10min and all standing with mice; Immediately mice is placed the diving tower appearance, opening power, the record mice successfully escapes electric shock and jumps onto the used time of platform (latent time).See table 3.

The influence of table 3 pair dizzy mice diving tower experiment

Annotate: compare with model group, ^*P＜0.05, ^*P＜0.01

Table 3 shows, 3 dosage of tablet of the present invention can shorten dizzy mice diving tower when hiding, and make dizzy mice jump onto platform rapidly for hiding electric shock, and present dose-effect relationship; And the model group mice prolongs when the mice diving tower is hidden because of not giving medicine.

The main pharmacodynamics of hard capsule of the present invention, soft capsule, drop pill, micropill etc. is close with the tablet experimental data, and conclusion is identical.

Specific embodiment

Embodiment 1: tablet of the present invention prepares like this: Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens, add 10 times of water, and steam distillation extracted volatile oil 2 hours, and the volatile oil of collection slowly joins in the saturated solution that an amount of HP-β-CD processes, and it is subsequent use to be prepared into clathrate; Aqueous solution behind the extraction volatile oil is subsequent use; Get above-mentioned weight portion Rhizoma Gastrodiae, the Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Caulis Bambusae In Taenia and Pericarpium Citri Reticulatae, the merging of Rhizoma Zingiberis Recens medicinal residues, boil 3 times, each 2 hours, filter with 8 times of decoctings; The aqueous solution of merging filtrate and above-mentioned Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens concentrates the back and goes up macroporous resin, and elder generation is with the water elution of 2 times of column volumes; The ethanol elution of 5 times of column volumes 50% of reuse is collected alcoholic acid eluent, and concentrating under reduced pressure becomes extractum; Vacuum drying obtains extract, and is subsequent use; Ramulus Uncariae Cum Uncis, Rhizoma Alismatis, the Rhizoma Pinelliae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Chuanxiong, with 50 times of 50% ethanol percolate extraction, percolate reclaims ethanol and concentrating under reduced pressure becomes extractum, and vacuum drying obtains extract, and is subsequent use; With the CMS-Na mixing of above-mentioned extract dry powder adding 15%, use the alcoholic solution of 5%PVP to be binding agent then, compacting promptly gets in flakes.

Embodiment 2: soft capsule of the present invention prepares like this: Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens, add 14 times of water, and steam distillation extracted volatile oil 1 hour, and the volatile oil of collection slowly joins in the saturated solution that an amount of HP-β-CD processes, and it is subsequent use to be prepared into clathrate; Aqueous solution behind the extraction volatile oil is subsequent use; Get above-mentioned weight portion Rhizoma Gastrodiae, the Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Caulis Bambusae In Taenia and Pericarpium Citri Reticulatae, the merging of Rhizoma Zingiberis Recens medicinal residues, boil 2 times, each 2 hours, filter with 12 times of decoctings; The aqueous solution of merging filtrate and above-mentioned Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens concentrates the back and goes up macroporous resin, and elder generation is with the water elution of 2 times of column volumes; The ethanol elution of 6 times of column volumes 60% of reuse is collected alcoholic acid eluent, and concentrating under reduced pressure becomes extractum; Vacuum drying obtains extract, and is subsequent use; Ramulus Uncariae Cum Uncis, Rhizoma Alismatis, the Rhizoma Pinelliae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Chuanxiong, with 100 times of 60% ethanol percolate extraction, percolate reclaims ethanol and concentrating under reduced pressure becomes extractum, and vacuum drying obtains extract, and is subsequent use; Add adjuvant 25% soybean oil then, add 5% Cera Flava, after heating makes dissolving, add above-mentioned spares, after stirring, colloid mill grinds well, and is pressed into soft capsule, promptly gets.

Embodiment 3: drop pill of the present invention prepares like this: Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens, add 10 times of water, and steam distillation extracted volatile oil 3 hours, and the volatile oil of collection slowly joins in the saturated solution that an amount of HP-β-CD processes, and it is subsequent use to be prepared into clathrate; Aqueous solution behind the extraction volatile oil is subsequent use; Get above-mentioned weight portion Rhizoma Gastrodiae, the Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Caulis Bambusae In Taenia and Pericarpium Citri Reticulatae, the merging of Rhizoma Zingiberis Recens medicinal residues, boil 2 times, each 1.5 hours, filter with 10 times of decoctings; The aqueous solution of merging filtrate and above-mentioned Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens concentrates the back and goes up macroporous resin, and elder generation is with the water elution of 2 times of column volumes; The ethanol elution of 6 times of column volumes 50% of reuse is collected alcoholic acid eluent, and concentrating under reduced pressure becomes extractum; Vacuum drying obtains extract, and is subsequent use; Ramulus Uncariae Cum Uncis, Rhizoma Alismatis, the Rhizoma Pinelliae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Chuanxiong, with 80 times of 50% ethanol percolate extraction, percolate reclaims ethanol and concentrating under reduced pressure becomes extractum, and vacuum drying obtains extract, and is subsequent use; With Macrogol 4000, put in the water-bath and to add above-mentioned spares behind the heating and melting, stirring is transferred to reservoir, airtight and in 70 ℃ of insulations, is coolant with the methyl-silicone oil, processes drop pill, promptly gets.

Embodiment 4: micropill of the present invention prepares like this: Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens, add 8 times of water, and steam distillation extracted volatile oil 4 hours, and the volatile oil of collection slowly joins in the saturated solution that an amount of HP-β-CD processes, and it is subsequent use to be prepared into clathrate; Aqueous solution behind the extraction volatile oil is subsequent use; Get above-mentioned weight portion Rhizoma Gastrodiae, the Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Caulis Bambusae In Taenia and Pericarpium Citri Reticulatae, the merging of Rhizoma Zingiberis Recens medicinal residues, boil 2 times, each 1 hour, filter with 10 times of decoctings; The aqueous solution of merging filtrate and above-mentioned Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens concentrates the back and goes up macroporous resin, and elder generation is with the water elution of 2 times of column volumes; The ethanol elution of 6 times of column volumes 50% of reuse is collected alcoholic acid eluent, and concentrating under reduced pressure becomes extractum; Vacuum drying obtains extract, and is subsequent use; Ramulus Uncariae Cum Uncis, Rhizoma Alismatis, the Rhizoma Pinelliae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Chuanxiong, with 70 times of 50% ethanol percolate extraction, percolate reclaims ethanol and concentrating under reduced pressure becomes extractum, and vacuum drying obtains extract, and is subsequent use; With above-mentioned spares and 8% little article fiber (MCC) mixing, using 80% ethanol liquid of 10% low-substituted hydroxypropyl cellulose (L-HPC) then is adhesive, through extrude roll the net comminutor process micropill, promptly get.

Embodiment 5: hard capsule of the present invention prepares like this: Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens, add 6 times of water, and steam distillation extracted volatile oil 4 hours, and the volatile oil of collection slowly joins in the saturated solution that an amount of HP-β-CD processes, and it is subsequent use to be prepared into clathrate; Aqueous solution behind the extraction volatile oil is subsequent use; Get above-mentioned weight portion Rhizoma Gastrodiae, the Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Caulis Bambusae In Taenia and Pericarpium Citri Reticulatae, the merging of Rhizoma Zingiberis Recens medicinal residues, boil 2 times, each 2 hours, filter with 14 times of decoctings; The aqueous solution of merging filtrate and above-mentioned Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens concentrates the back and goes up macroporous resin, and elder generation is with the water elution of 1 times of column volume; The ethanol elution of 4 times of column volumes 40% of reuse is collected alcoholic acid eluent, and concentrating under reduced pressure becomes extractum; Vacuum drying obtains extract, and is subsequent use; Ramulus Uncariae Cum Uncis, Rhizoma Alismatis, the Rhizoma Pinelliae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Chuanxiong, with 90 times of 40% ethanol percolate extraction, percolate reclaims ethanol and concentrating under reduced pressure becomes extractum, and vacuum drying obtains extract, and is subsequent use; With above-mentioned spares and maltodextrin, little article fiber (MCC), the calcium stearate mixing is packed in the glue shell, promptly gets.

Claims (1)

1. be used to treat the Chinese medicine preparation of dizziness; It is characterized in that: it is by Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens volatile oil clathrate 0.2-0.4 part; Extract 2-8 part of Rhizoma Gastrodiae, the Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Caulis Bambusae In Taenia and Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens medicinal residues; Extract 2-8 part of Ramulus Uncariae Cum Uncis, Rhizoma Alismatis, the Rhizoma Pinelliae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Chuanxiong and required adjuvant are prepared from, and its preparation method is:

(1) raw material medicines in portions by weight consists of: 5 parts in Rhizoma Gastrodiae, 20 parts of 10 portions of Rhizoma Alismatis of Ramulus Uncariae Cum Uncis (system), 10 parts of Rhizoma Pinelliae (processed), 12 parts of the Rhizoma Atractylodis Macrocephalaes, 12 parts in Poria, 10 parts of the Radix Paeoniae Albas, 10 parts of Caulis Bambusae In Taenia, 3 parts of Rhizoma Chuanxiongs, 3 parts in Radix Glycyrrhizae (processing), 6 parts of Pericarpium Citri Reticulataes, Rhizoma Zingiberis Recens 4-5 part;

(2) get above-mentioned weight portion Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens, add 6-14 times of water, steam distillation extracted volatile oil 1-5 hour, and the volatile oil of collection slowly joins in the saturated solution that an amount of HP-β-CD processes, and it is subsequent use to be prepared into clathrate; Aqueous solution behind the extraction volatile oil is subsequent use; Get above-mentioned weight portion Rhizoma Gastrodiae, the Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Caulis Bambusae In Taenia and Pericarpium Citri Reticulatae, the merging of Rhizoma Zingiberis Recens medicinal residues, boil 2-3 time, each 1-2 hour, filter with 8-14 times of decocting; The aqueous solution of merging filtrate and above-mentioned Pericarpium Citri Reticulatae, Rhizoma Zingiberis Recens concentrates the back and goes up macroporous resin, and elder generation is with the water elution of 1-2 times of column volume; The ethanol elution of reuse 4-6 times of column volume 40-60% is collected alcoholic acid eluent, and concentrating under reduced pressure becomes extractum; Vacuum drying obtains extract, and is subsequent use;

(3) get above-mentioned weight portion Ramulus Uncariae Cum Uncis, Rhizoma Alismatis, the Rhizoma Pinelliae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Chuanxiong, with 50-100 times of 40-60% ethanol percolate extraction, percolate reclaims ethanol and concentrating under reduced pressure becomes extractum, and vacuum drying obtains extract, and is subsequent use;

(4) get the required adjuvant of above-mentioned clathrate and extract adding and can be made into preparations such as tablet, hard capsule, soft capsule, drop pill, micropill.