TWI409076B - Medical herb composition for increasing inflammation, swell and pain, method for manufacturing medical herb abstract, application for the same - Google Patents

Abstract

A medical herb composition for increasing inflammation, swell and pain includes first and herb materials. The first herb material includes Bletillae Rhizoma, Cinnamomi Cortex, Angelicae Dahuricae Radix, Angelicae sinensis Radix, Paeoniae Radix, Notopterygii Rhizoma Et Radix, Linderae Radix, Glycyrrhizae Radix, Angelicae pubescentis Radix, and Rheiradix Et Rhizoma. The second herb material is selected from one, two or three of the group consisting of Zingiber officinale, Qlibanum and Myrrha. Additional material (Zingiber officinale, Qlibanum and Myrrha) of the medical herb composition of the present invention can increase curative effect. In addition, the present invention discloses the process for manufacturing abstract. For example, the first and herb materials and an organic solvent are put into a vessel, is heated and extracted, is filtered during heating. Then, the extracted filtrate is concentrated to an extract. Compared with the conventional prescription, the relative steps of the process for manufacturing abstract of the medical herb composition of the present invention have no disadvantage of the conventional prescription.

Description

Medicinal composition for anti-inflammatory, swelling and pain relief, preparation method and application thereof

The invention relates to a herbal composition for anti-inflammatory, swelling and analgesic, and more particularly to an herbal extract for anti-inflammatory, swelling and analgesic, a preparation method and application thereof.

Because modern people's lives are getting more and more intense, busy, and there is not much time for exercise, plus the result of standing or sitting for a long time. Therefore, modern people often have many civilized diseases, such as inflammation of the nerves or muscles, muscle soreness and other symptoms. If it is neglected, it will cause a vicious circle and qi and blood.

Since ancient times, Chinese people have used Chinese herbal medicine for thousands of years of experience in the treatment of diseases. They have been treating foreign diseases in a way that is solid and resistant to evil, and have solved the problem of internal inflammation or soreness, so that Chinese herbal medicine can continue to this day.

At present, Chinese herbal medicine manufacturers have developed Chinese herbal medicine patches in order to meet the convenience needs of modern people. The Chinese herbal patch has the convenience of external treatment, and is only applied to the skin at a position where the body is sore or uncomfortable, and the ingredients of the Chinese herbal patch are infiltrated into the human skin, and the effect is gradually exerted.

Wanying cream has been used to treat body aches and discomforts since ancient times, so it has been recognized for a long time. Its formula is composed of Chuanwu, Muzizi, Caowu, Rehmannia, Radix, Radix Paeoniae Alba. 17 kinds of medicinal herbs such as cinnamon, white peony, angelica, red peony, medlar, sophora, black medicinal herbs, licorice, solo, yuan ginseng and rhubarb. However, some of the medicinal materials, such as Chuanwu, Muxizi and Caowu, have been proven to be toxic and skin irritating, so they are prone to skin discomfort after use, as shown in Table 1 below.

In addition, Gufang Wanying Ointment uses sesame oil to extract medicinal ingredients, which is easy to destroy the ingredients in the medicinal materials due to excessive temperature, or the extraction of active ingredients is not complete, the quality control is not easy, and the problem of heavy metal residue is caused by adding a large amount of zinc oxide during molding.

Therefore, it is necessary to provide a herbal composition for anti-inflammatory, swelling, and analgesic, which can solve the aforementioned problems.

The invention provides a herbal composition for anti-inflammatory, swelling and analgesic, comprising a first medicinal material and a second medicinal material. The first type of medicinal materials include white peony, cinnamon, white peony, angelica, peony, scorpion, black medicinal herb, licorice, solitary, and rhubarb. The second type of medicinal material is selected from one, two or three of the group consisting of ginger, frankincense and myrrh.

The herbal composition for anti-inflammatory, swelling and pain relief of the present invention does not include Chuanwu, Muzizi, Caowu, Rehmannia, Radix Paeoniae Alba, Sophora flavescens and Yuanshen. Therefore, the herbal composition for anti-inflammatory, swelling, and analgesic of the present invention is completely different from the inherent formulation of Wanying Cream. Furthermore, since Chuanwu, Muxizi and Caowula have been confirmed to be toxic and skin irritating, the herbal composition of the present invention does not have the disadvantages of the inherent formulation of Wanying Cream. In addition, compared with the inherent formulation of Wanying Cream, the herbal composition of the present invention further comprises an added medicinal material (ginger, frankincense and myrrh) to increase its therapeutic effect.

The invention selects the effective Chinese herbal medicine which has no irritation and allergic effect on the skin and the human body; the special extraction, preparation method and the animal test method verify the anti-inflammatory swelling and pain relief effect of the invention, which is different from the general traditional Wanying cream And the commercially available traditional Chinese medicine patch, which will be described in detail below with the accompanying drawings.

The invention provides a herbal composition for anti-inflammatory, swelling and analgesic, which comprises a first medicinal material and a second medicinal material. The first type of medicinal material comprises white peony, cinnamon, white peony, angelica, peony (such as red peony), medlar, black medicinal herb, licorice, lycorrhea, rhubarb, wherein the first medicinal material is a basic medicinal material. The second type of medicinal material is selected from one, two or three of the group consisting of ginger, frankincense and myrrh, wherein the second medicinal material is an added medicinal material. In detail, the herbal composition of the present invention comprises seven kinds of medicinal materials arranged in combination in Table 2 below.

When the second type of medicinal material is selected from the group consisting of ginger, frankincense and myrrh, the Chinese herbal medicine composition comprises 11 medicinal materials, and the weight percentage of each medicinal material is in the range of 9.09%±5%. . When the second type of medicinal material is selected from the group consisting of ginger, frankincense and myrrh, the Chinese herbal medicine composition comprises 12 medicinal materials, and the weight percentage of each medicinal material is in the range of 8.33%±5%. . When the second type of medicinal material is selected from the group consisting of ginger, frankincense and myrrh, the Chinese herbal medicine composition comprises 13 medicinal materials, and the weight percentage of each medicinal material is within the range of 7.69%±5%. .

The first type of medicinal material of the present invention is a basic medicinal material, and the second medicinal material is an added medicinal material. The medicinal materials of the seven kinds of arrangement and combination in Table 2 can reach the therapeutic effect of the invention. The invention only uses the seventh combination: the Chinese herbal medicine composition contains 13 herbs (ie, white peony, cinnamon, white peony, angelica, peony, medlar, black medicinal herb, licorice, lycox, rhubarb, ginger, frankincense and myrrh) The following is a description of the embodiments, and the other six combinations can also be implemented in a similar manner, and will not be described again.

A comparison of the formulation of the anti-inflammatory, anti-inflammatory and analgesic herbal composition of the present invention with the intrinsic formula of the Wanying cream is shown in Table 3 below.

The herbal composition for anti-inflammatory, swelling and pain relief of the present invention does not include Chuanwu, Muzizi, Caowu, Rehmannia, Radix Paeoniae Alba, Sophora flavescens and Yuanshen. Therefore, the herbal composition for anti-inflammatory, swelling, and analgesic of the present invention is completely different from the inherent formulation of Wanying Cream. Furthermore, since Chuanwu, Muxizi and Caowula have been confirmed to be toxic and skin irritating, the herbal composition of the present invention does not have the disadvantages of the inherent formulation of Wanying Cream. In addition, compared with the inherent formulation of Wanying Cream, the herbal composition of the present invention further comprises an added medicinal material (ginger, frankincense and myrrh) to increase its therapeutic effect.

According to the present invention, the documentary efficacy of the medicinal material for the anti-inflammatory, swelling and analgesic herbal composition is as shown in Table 4 below.

The herbal extract of the present invention can be obtained by the production method of the first embodiment of the present invention. Referring to FIG. 1, the preparation method of the first embodiment comprises the following steps: In step 100, a first type of medicinal material and a second medicinal material are provided, wherein the first medicinal material comprises white peony, cinnamon, white peony, angelica, Peony (eg, red peony, white peony), medlar, black medicinal herb, licorice, lycoxual, rhubarb, the first medicinal material is the basic medicinal material, and the second medicinal material is selected from ginger (eg dried ginger, ginger), Frankincense and myrrh, the second type of medicine is added herbs. In step 102, an organic solvent (such as methanol, ethanol or acetone, methyl ethyl ketone, kerosene (petroleum ether), hexane, etc.) is added, and the first and second medicinal materials are placed in the organic solvent. In a first container (ie, an extraction barrel), the weight percentage of the first and second types of medicinal materials and the organic solvent is 1:N to form a mixed solution, wherein N is between 3 and 12 . Preferably, N is 8. In step 104, the mixture is heated to a predetermined temperature and extracted for a predetermined time to perform extraction, wherein the predetermined temperature is between 30 ° C and 100 ° C. When the organic solvent is ethanol (i.e., alcohol), it is preferred that the predetermined temperature is in the range of 5 ° C of the boiling point of the ethanol. The predetermined time is between 1 and 6 hours. In step 106, the mixture is filtered while hot, and the extracted filtrate is taken to a second container (i.e., a measuring cup). In step 108, the extracted filtrate is concentrated to an extract having a water content of 10% to 40% and an organic solvent content of 3% to 30%. Preferably, the extracted filtrate is concentrated to an extract having a water content of 10% to 20% and an organic solvent content of 5% to 10%. The extract is an intermediate product that can be easily shipped and sold for subsequent final products. In step 110, the extracted residue is removed from the container.

The herbal extract of the present invention can also be obtained by the production method of the second embodiment of the present invention. Referring to FIG. 2, the preparation method of the second embodiment comprises the following steps: In step 200, a first type of medicine and a second type of medicine are provided, wherein the first type of medicine comprises white peony, cinnamon, white peony, angelica, The first type of medicinal material is selected from the group consisting of ginger, frankincense and myrrh, and the second type of medicinal material is added medicinal material. In step 202, an organic solvent is added, and the first and second types of medicinal materials and the organic solvent are placed in a first container (ie, an extraction barrel) to make the first and second medicinal materials and the organic The weight percentage of solvent is 1:N to form a first mixture, where N is between 3 and 12. Preferably, N is 8. In step 204, the first mixed liquid is heated to a first predetermined temperature and extracted for a first predetermined time to perform a first extraction, wherein the first predetermined temperature is between 30 ° C and 100 ° C. When the organic solvent is ethanol, preferably the first predetermined temperature is in the range of 5 ° C of the boiling point of the ethanol. The first predetermined time is between 1 and 6 hours. In step 206, the first mixture is filtered while hot, and the first extracted filtrate is taken to a second container (i.e., a measuring cup). In step 208, the residue of the first extraction is retained. In step 210, the organic solvent is re-added, and the weight percentage of the medicinal material and the organic solvent is returned to 1:N to form a second mixed solution, wherein N is between 3 and 12. Preferably, N is 8. In step 212, the second mixed liquid is heated to a second predetermined temperature and extracted for a second predetermined time to perform a second extraction, wherein the second predetermined temperature is between 30 ° C and 100 ° C. When the organic solvent is ethanol, preferably the second predetermined temperature is in the range of 5 ° C of the boiling point of the ethanol. The second predetermined time is between 1 and 6 hours. In step 214, the second mixture is filtered while hot, and the second extracted filtrate is taken. In step 216, the first extracted filtrate and the second extracted filtrate are mixed. In step 218, the mixed filtrate is concentrated to an extract having a water content of 10% to 40% and an organic solvent content of 3% to 30%. Preferably, the mixed filtrate is concentrated to an extract having a water content of 10% to 20% and an organic solvent content of 5% to 10%. The extract is an intermediate product that can be easily shipped and sold for subsequent final products. In step 220, the residue from the second extraction is removed from the container.

Referring to Fig. 3, the herbal extract of the present invention can be applied to a Chinese herbal patch 300. The herbal patch 300 comprises a capsule layer 310 and a herbal medicine layer 320. The herbal medicine layer 320 is disposed on the capsule layer 310. In this embodiment, the herbal medicine layer comprises a diluent and an extract of the herbal extract of the present invention. The extract of the Chinese herbal extract (intermediate product) is incorporated into the diluent so as to form the herbal medicine layer (final product). The diluent comprises ethanol, water and an aqueous binder. The Chinese herbal patch 300 has a Chinese herbal extract content of 50 to 3500 mg/14 g (each herbal patch).

Referring to Figure 4, the herbal extract of the present invention can be applied to a Chinese herbal spray. In this embodiment, the herbal spray 400 comprises a diluent and an extract of the herbal extract of the present invention. The extract of the Chinese herbal extract (intermediate product) is incorporated into the diluent so as to form the herbal spray (final product). The diluent comprises ethanol, polyols, water. The Chinese herbal medicine spray 400 has a Chinese herbal medicine extract content of 10 to 500 mg/g. In another embodiment, the Chinese herbal medicine spray does not need to contain the concentrated extract (intermediate product), but includes the extraction filtrate (also an intermediate product) before concentration, thus reducing the concentration step, thereby reducing the process time and cost. .

Referring to Fig. 5, in the present embodiment, the Chinese herbal medicine extract can be applied to the Chinese herbal medicine cream 500, and the ointment can be further divided into an ointment, a water-based gel and a cream. The herbal ointment 500 comprises a diluent and an extract of the herbal extract of the present invention. The extract of the Chinese herbal extract (intermediate product) is incorporated into the diluent so as to form the herbal ointment (final product). The diluent contains ointment, white wax oil and nonionic surfactant; the aqueous gel contains ethanol, water and nonionic surfactant; the cream contains grease, wax and emulsifier. The Chinese herbal medicine ointment 500 has a Chinese herbal medicine extract content of 10 to 500 mg/g.

Furthermore, the herbal extract of the present invention can also be applied to an internal preparation, and the internal preparation can be further divided into a powder, a pill and a lozenge. In this embodiment, the internal preparation comprises a diluent and an extract of the herbal extract of the present invention. The extract of the Chinese herbal extract (intermediate product) is incorporated into the diluent so as to form the internal preparation (final product). The diluent of the powder and the pill contains starch and sugar (such as sugar or honey); the diluent of the tablet contains crystalline cellulose. The internal preparation has a content of a traditional Chinese medicine extract of 3 to 250 mg/g.

In addition, the above-mentioned herbal extract contains the following nine main indicator components: Paeoniflorin, Ferulic acid, Cinnamaldehyde, Glycyrrhizin, Rhein, and Eucalyptus. Imperatorin, Osthol, Isoimperatorin, and Gingerol, in which the content of the indicator components per 1 g of extract is: Paeoniflorin 4.466~1.488mg, ferulic acid (0.33~0.127mg), Cinnamaldehyde 2.19~0.720mg, Glycyrrhizin 9.767~3.226mg, Rhein 1.013~0.338mg, Europe Imperatorin 0.727~0.242mg, Osthol 1.389~0.463mg, Isoimperatorin 0.709~0.236mg and Gingerol 0.144~0.432mg.

Experimental examples and data of the present invention:

A. Extracting extracts of various medicinal materials for animal skin irritation test:

The intrinsic formula of Wanying paste and the medicinal materials of the herbal composition of the present invention and 95% ethanol were extracted by a turbulent flow extraction device at a weight ratio of 1:8 at 80 ° C for 3 hours, repeated twice, and two times of extract filtration were collected. Thereafter, it was concentrated into a extract by a vacuum condenser. 25 μl of the extract and positive and negative controls were administered to the skin of the animal with the back hair removed. After 24 hours of stimulation, the skin reaction was observed at 24, 48 and 78 hours after the recording, and scored according to the skin irritation scoring system. The method (Table 5 below) and the Primary Dermal Irritation Index (Table 6 below) were used to assess the degree of skin irritation (Table 7 below).

Table 7 Skin irritation

As can be seen from Table 7, although some of the medicinal materials of the herbal composition of the present invention have weak irritation, the extract of the present invention is not irritating. However, Wan Ying cream is moderately irritating.

B. The preferred extraction conditions of the herbal composition of the present invention are:

In the herbal composition of the present invention, 13 kinds of medicinal materials are mixed in equal proportion or in various proportions, and then extracted and concentrated under different conditions (temperature, organic solvent), and subjected to high performance liquid chromatography (HPLC) test after appropriate dilution. And calculate the content of the index components (peony, angelica, cinnamon, licorice, rhubarb, white peony, singular, scorpion), compare the extraction results of different medicinal materials, and select the best medicinal material extraction conditions, as shown in Table 8 below.

The results are as follows: Table 9: High-performance liquid chromatography (HPIC) calculated under the different conditions, the average content of the index components contained in the extract of each 1g compound medicinal material (n = 3 times), the unit is mg, from the following table 9 It can be seen that 95%~50% ethanol or higher temperature can extract more index components.

C. High performance liquid chromatography (HPLC) analysis of each medicinal material of the herbal composition of the present invention:

1 g of each medicinal material and 8 g of 95% ethanol were added to the turbulent flow extraction apparatus for extraction at 80 ° C for 3 hours, repeated twice, and the extract was collected twice, filtered, and concentrated into a extract by a vacuum concentrator, and quantified by methanol (MeOH). To 150.0 ml as a test solution, 10 μl of the test solution was subjected to high performance liquid chromatography (HPLC) for component analysis and fingerprinting was established as shown in Table 10 below.

D. High performance liquid chromatography (HPLC) analysis of the formulation of the herbal composition of the present invention:

The herbal composition of the present invention is prepared by mixing 1 g of each medicinal material with 13 g, and adding 104 g of 95% ethanol to the turbulent flow extraction device for extraction at 80 ° C for 3 hours, taking the filtrate, and repeating the residue according to the above procedure, collecting 2 times of extraction. After filtering the liquid, it was concentrated into an extract by a vacuum concentrator, and the mixture was quantified to 150.0 ml with methanol (MeOH) as a test solution, and 10 μl of the test solution was subjected to high performance liquid chromatography (HPLC) for component analysis and fingerprinting. See Table 11 below.

E. Transdermal test of the medicinal ingredient extract of the present invention:

The test animals were Wistar rats (weight about 250 g). During the test, the feed water was taken. The test sample is a concentrated extraction extract of a Chinese herbal medicine composition. The mouse was sacrificed before the cervical spine was broken, and the abdominal hair was shaved with an electric shaver to remove the abdominal skin. The skin was removed and the inside was moistened with physiological saline and sandwiched between a Donor cell and a Receptor cell of a transdermal Franz Cell System. The test samples were dissolved in a suitable solvent. And placed in the Donor cell. The Receptor cell was filled with physiological saline (containing 20% PEG400, polyethylene glycol 400) at a constant temperature of 37 ° C and a fixed speed stirring at 500 rpm for skin penetration test, and samples were taken at 3, 6, 12, 24, 36 and 72 hours. For the indicator components of the Chinese herbal patch, the sample was injected into high performance liquid chromatography (HPLC) for content determination.

As a result, the transdermal components detectable by high performance liquid chromatography (HPLC) are components of medicinal materials such as angelica, peony, and cinnamon, and include the index components of the above three medicinal materials, as shown in Table 12 below.

F. Analgesic experiment of the herbal patch of the invention:

First, acetic acid writhing method:

Principle: If chemical substances are used to stimulate animals, such as: acetic acid (acetic acid), bradykinin (bradykinin), K ⁺ , etc., intraperitoneal injections stimulate the peritoneum or contact and skin cause stimulation of chemically sensitive receptors to trigger pain response, showing abdominal contraction A writhing manifestation such as invagination, hind limb extension, body twisting or creeping.

The test was initiated by ICR mice (male; 6-8 weeks old). During the test, the feed water was taken. And fasted for 24 hours before the test. The trials were divided into three groups, the negative control group (without any drug patch), the positive control group (commercially available Indomethacin & Diclofenac sodium) and the experiment. Group (4 different doses of drug patch for the experiment), 12 in each group.

The mice were weighed and numbered, and then the limbs were fixed on the flat plate and shaved in the abdomen, and the patch (2 x 3 cm) was attached to the abdomen of the mouse. After 3 hours, the drug patch was removed (or the drug patch was not removed) and intraperitoneally injected (i.p., 20 mL/kg) with 0.6% acetic acid solution. Observe the number of twistings of the mouse within 10 minutes and record the wringing mean, as shown in Table 13 below.

As a result, the twisted average value of the herbal patch of the present invention is smaller than the twisted average of the negative control group (the drug patch without any drug), and therefore the herbal patch of the present invention does have an analgesic effect.

Second, formalin (formalin) test method:

Principle: Subcutaneous injection of diluted formalin in rats, moles can observe the pain response of the two phases of the central and peripheral nervous system. This type of Fumalin foot test was first proposed by Dubuisson and Dennis in 1977, which is many The screening of analgesic drugs is an effective and reliable model. When subcutaneously injected into the human skin by subcutaneous injection, it will cause a strong and sharp burning sensation in 4-5 minutes, and there will be continuous pain in the next 30-60 minutes. It is manifested in the behavior of the moles caused by the injection of the formalin stimulation and the lameness or foot-up behavior. The initial injection of formalin caused a painful reaction at 0-5 points. The lame time spent is called the early phase, mainly due to the direct stimulation of the pain response receptor causing substance P (substance P), bradykinin (bradykinin) release and painful response; The amount of lameness spent in 40 minutes is called late, mainly due to inflammatory reactions that cause chemical mediators such as histamine, serotonin, prostaglandin, and kinin. Kinin), etc., caused by the release of damaged tissue cells. In short. The effect of the analgesic drug activity and mechanism of action of inflammatory and non-inflammatory pain can be effectively evaluated by the effect of formalin-induced pain and crappy behavior in mice. In addition, the concentration of pain induced by formalin is an important factor. When it is 0.02~0.2%, only the pre-clam foot reaction is induced, and the tissue is identified by light microscopy. The change is small; Higher levels of formalin can induce pre- and late lameness reactions. If flumarin is injected 5%, acute inflammatory reaction can be found in tissue identification after 30 minutes, and particle ball destruction and swelling can be seen.

The test animals were ICR mice (male; 6-8 weeks old). During the test, the feed water was taken. And fasted for 24 hours before the test. The trial was divided into three groups, the negative control group (drug patch without any drug), the positive control group [commercially available Indomethacin & Diclofenac sodium drug patch] and experiment Group (4 different doses of herbal patch) for each group, 12 in each group.

The mouse was weighed and numbered. The medicine patch (2×3cm) was cut into four equal parts and attached to the instep of the mouse. Then it was wrapped with air-permeable tape. After 3 hours, the medicine patch was removed and the day was prepared. The % formalin solution was subcutaneously injected with 20 μl on the instep, and the lameness time of the mice during 0-5 minutes and 15-40 minutes was calculated as shown in Table 14 below.

As a result, the lameness time of the herbal patch of the present invention is less than the lameness time of the negative control group (the drug patch without any drug), and therefore the herbal patch of the present invention does have an analgesic effect.

G. The anti-inflammatory and swelling test of the herbal patch of the invention:

Principle: The edema caused by γ-carrageenin injection into the ankle is a biphasic reaction. After injection of γ-carrageenin, different substances are released at different times to cause inflammation and swelling, ie 0-1.5 hours or 20 minutes to 1 hour (first phase )histamine (histamine), serotonin (serotonin), PAF (platelet activating factor) (platelet activating factor) will be released; 1.5-2.5 hours (second phase), kinin (kinin) substances will be released, after 2.5 hours (third phase), prostaglandin (prostaglandin) and leukotriens (leucotriene) are released to cause inflammation and swelling.

The test was performed in SD rats (male; 6-8 weeks old). During the test, the feed water was taken. And fasted for 24 hours before the test. The trial was divided into three groups, the negative control group (drug patch without any drug), the positive control group [commercially available Indomethacin & Diclofenac sodium drug patch] and experiment Group (4 different doses of drug patch for the experiment), 8 in each group.

The rats were weighed and numbered, and the "white line" was drawn with a signature pen on the ankles of the rats. The volume of the ankle was measured first with a Plethysmometer. Wear a self-made (different size) corset elastic bandage to prevent the mouse from biting off the Chinese herbal patch. Each group of mice was injected subcutaneously with 0.1 ml of Carrageenan (carrageenan) (10 mg/ml in saline) in the athlete's foot. Positive control group and experimental group paste Chinese herbal patch (2 × 3cm) on the ankle, and wrapped with breathable tape, with the amount of swelling tester 0, 2, 4, 6 and 24 hours, etc. (required to be updated after different time measurement) Percentage of swelling of Chinese herbal patch), observe the swelling at each time point and draw out whether there is a biometric difference between the swelling curve of the experimental group and the control group, as shown in Table 15 below.

As a result, the percentage of edema of the herbal patch of the present invention is less than the percentage of edema of the negative control group (the drug patch without any drug), and therefore the herbal patch of the present invention does have the effect of reducing inflammation and swelling.

The present invention has been disclosed in the foregoing embodiments, and is not intended to limit the present invention. Any of the ordinary skill in the art to which the invention pertains can be modified and modified without departing from the spirit and scope of the invention. . Therefore, the scope of the invention is defined by the scope of the appended claims.

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300. . . Chinese herbal patch

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500. . . Chinese herbal ointment

Fig. 1 is a flow chart showing the preparation method of the first embodiment of the present invention.

Fig. 2 is a flow chart showing the preparation method of the second embodiment of the present invention.

Fig. 3 is a perspective view showing the herbal patch of the present invention.

Fig. 4 is a schematic cross-sectional view showing the herbal spray of the present invention.

Fig. 5 is a perspective view showing the herbal ointment of the present invention.

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Claims (24)

A Chinese herbal medicine composition comprising: a first type of medicinal material, comprising white peony, cinnamon, white peony, angelica, peony, medlar, black medicinal herb, licorice, lycorrhea, rhubarb, and the first medicinal material substantially does not contain Chuanwu, a medicinal material selected from the group consisting of ginger, frankincense, and myrrh, wherein the weight percentage of each of the above 11 herbs is 9.09% ± Within 5%. A Chinese herbal medicine composition comprising: a first type of medicinal material, comprising white peony, cinnamon, white peony, angelica, peony, medlar, black medicinal herb, licorice, lycorrhea, rhubarb, and the first medicinal material substantially does not contain Chuanwu, a medicinal material selected from the group consisting of ginger, frankincense, and myrrh, wherein the weight percentage of each of the above 12 herbs is 8.33% ± Within 5%. A Chinese herbal medicine composition comprising: a first type of medicinal material comprising white peony, cinnamon, white peony, angelica, peony, medlar, black medicinal herb, licorice, lycox, rhubarb, and the One type of medicinal material does not substantially contain Chuanwu, Muxizi and Caowu; and a second type of medicinal material containing ginger, frankincense and myrrh, wherein the weight percentage of each of the above 13 medicinal materials is 7.69%±5% Within the scope. A Chinese herbal medicine extract obtained by the following steps: providing a first medicinal material and a second medicinal material, wherein the first medicinal material comprises white peony, cinnamon, white peony, angelica, peony, peony, black medicinal herb, licorice The first type of medicinal material is a basic medicinal material and substantially does not include Chuanwu, Muxizi and Caowu. The second medicinal material is selected from the group consisting of ginger, frankincense and myrrh. In one, two or three, the second type of medicinal material is an added medicinal material; the first and second medicinal materials and an organic solvent are placed in a container to make the first and second medicinal materials and the organic solvent The weight percentage is 1:N to form a first mixed liquid, wherein N is a value between 3 and 12; heating the first mixed liquid to between 30 ° C and 100 ° C and extracting for 1 to 6 hours, The first extraction is carried out; and the first mixed liquid is filtered while hot, and the filtrate of the first extraction is taken. According to the fourth patent application of the patent scope, the following steps are included: The filtrate extracted for the first time is concentrated to an extract having a water content of 10% to 40% and an organic solvent content of 3% to 30%. According to the fifth patent of the patent application scope, the herbal extract is obtained by the following steps: retaining the residue of the first extraction; re-adding the organic solvent to return the weight percentage of the medicinal material to the organic solvent back to 1: N, to form a second mixture, wherein N is between 3 and 12; heating the second mixture to between 30 ° C and 100 ° C and extracting for 1 to 6 hours for the second extraction; The second mixture was filtered while hot, and the filtrate from the second extraction was taken. According to the sixth patent of the patent application scope, the herbal extract is obtained by the following steps: mixing the first extraction filtrate with the second extraction filtrate, and concentrating the mixed filtrate to a water content of 10%~40 %, the organic solvent content of 3% to 30% of the extract. The herbal extract according to item 4 of the patent application scope, wherein the organic solvent is at least one selected from the group consisting of methanol, ethanol, acetone, methyl ethyl ketone, kerosene (petroleum ether) and hexane. According to the fourth aspect of the patent application, the organic solvent is ethanol, and the first mixed liquid is heated to a boiling point of ethanol of 5 ° C. According to the sixth aspect of the patent application, the organic solvent is ethanol, and the first and second mixed liquids are heated to a boiling point of ethanol of 5 ° C. According to the fourth patent application of the patent scope, the N is 8. According to the fourth patent application of the patent scope, the following nine indicators are included: Paeoniflorin, Ferulic acid, Cinnamaldehyde, Glycyrrhizin, Rhubarb Acid (Rhein), Imperatorin, Osthol, Isoimperatorin, and Gingerol. According to the 12th Chinese herbal medicine extract of the patent application scope, the content of the index components contained in each 1 g of extract is: Paeoniflorin 4.466~1.488mg, Ferulic acid 0.382~0.127mg , Cinnamaldehyde 2.159~0.720mg, Glycyrrhizin 9.677~3.226mg, Rhein 1.013~0.338mg, Imperatorin 0.727~0.242mg, Osthol 1.389~0.463mg, Isoimperatorin 0.709~0.236mg and Gingerol 0.144~0.432mg. A Chinese herbal patch containing: a layer of tape; and a layer of herbal medicine disposed on the layer of tape, wherein the layer of herbal medicine comprises the extract of claim 5 or 7. According to the patent application scope of claim 14, the herbal medicine layer further comprises a diluent composed of ethanol, water and an aqueous adhesive, and the extract is incorporated into the diluent. According to the 15th item of the patent application, the Chinese herbal patch has a Chinese herbal extract content of 50~3500mg/14g. A Chinese herbal medicine spray comprising the extracted filtrate as described in claim 4 of the patent application. A Chinese herbal medicine spray comprising the extract as described in claim 5 of the patent application. A Chinese herbal ointment comprising the extract as described in claim 5 or 7. According to claim 19, the herbal ointment further comprises: a diluent, wherein the extract is incorporated into the diluent, the diluent comprising ethanol, water and an aqueous viscose. According to the 20th item of the patent application, the Chinese herbal ointment is one of an ointment, a water gel and a cream. An internal preparation comprising, as claimed in claim 5 or 7 The extract described in the item. According to the internal preparation of claim 22, the method further comprises: a diluent, wherein the extract is incorporated into the diluent, the diluent comprising ethanol, water and an aqueous viscose. Oral preparation according to item 23 of the patent application scope, wherein the internal preparation is one of a powder, a pill and a lozenge.