CN102302555A - Chinese medicinal extract for treating urarthritis, as well as preparation method and application thereof - Google Patents
Chinese medicinal extract for treating urarthritis, as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a Chinese medicinal extract for treating urarthritis, and the Chinese medicinal extract is characterized by being prepared from the following raw material drugs: Illicium henryi root bark, Illicium henryi root, Illicium henryi stem leaves, anisetree bark, Illicium jiadifengpi or albizia julibrissin, wherein the content of total flavonoid in the extract is between 20 and 80 percent. The invention also provides a preparation method and application of the Chinese medicinal extract, as well as a preparation method and application of quercetin. The invention has the advantages that: pharmacological tests prove that the extract mainly prepared from illicium plant has remarkable prevention and treatment effects on acute urarthritis, does not have toxic or side effect, and can be used for preparing medicaments for preventing and treating urarthritis, and rheumatic or rheumatoid arthritis; new application of the quercetin in urarthritis treatment is discovered; according to the preparation method provided by the invention, toxic components are removed, active components are concentrated and remained, the application curative effect and safety are improved, thereby facilitating the safe application in medical industry and related industries.
Description
Technical field
The present invention relates to a kind of Chinese medicine extract, specifically, be a kind of be the Chinese medicine extract and its production and application of the treatment gouty arthritis of feedstock production with the Illicium plant.
Background technology
Gout (Gout) is that long-term purine metabolism obstacle, blood uric acid increase the one group of disease that causes tissue injury.Clinical characters is arthritis, tophus, urinary system calculus and gouty nephropathy.Wherein (Gouty arthritis, GA) comparatively common, it is because the blood uric acid excessive concentration that causes of purine metabolism obstacle causes a kind of metabolic joint disease of crystallization deposition due to the joint with gouty arthritis.Gout is in rising trend at the sickness rate of China.Acute gouty arthritis is not still had the way of radical cure, and the drug main that is used to regulate uric acid metabolism clinically will have allopurinol, probenecid etc., and the medicine that is used to treat acute gouty arthritis has colchicine, nonsteroidal anti-inflammatory agent and glucocorticoid etc.All deposit certain toxicity and side effect.Seek safe and effective medicine and be still a difficult problem.The mechanism of its inflammatory reaction is recently further illustrated.On the traditional Chinese medical science this disease is called " gout ", " arthralgia aggravated by cold ", " wind heat numbness " etc., thinks and mainly gather meridians in body because of damp and hot expectorant stasis of blood etc.; Or exopathogen invasion and attack skin and flesh passages through which vital energy circulates, cause mechanism of qi and do not declare, strongly fragrant and give birth to heat, multiple abscess limbs, muscle arteries and veins, joint and fall ill.Li Dongyuan thinks that gout belongs to blood deficiency more, due to cold and heat are able to invade then.Control suitable heat-clearing and toxic substances removing, removing dampness is carried throughout, is aided with activating blood and removing stasis Method.The Chinese medicine gout has significant advantage, and toxic and side effects is less.
Folium illicii Lanceolati has another name called the lanceolata Fructus Foeniculi, narrow leaf Fructus Foeniculi
Illicium lanceolatumA. C. Smith is Winteraceae Illicium plant, and its root or root bark are used as medicine, and the beginning is stated from Shennong's Herbal, and the effect of wind-expelling pain-stopping, dispersing swelling and dissipating binds, killing parasites for relieving itching is arranged.The medical material name is called Radix Illicii Lanceolati.Mainly be distributed in many areas on the south China the Changjiang river, have rich in natural resources.Among the people with Folium illicii Lanceolati radical cure treatment traumatic injury, lumbago caused by internal damage.The lanceolata Fructus Foeniculi is distributed in areass to the south, the Changjiang river such as Shaanxi, Jiangsu, Anhui, Zhejiang, Jiangxi.Its fruit often is considered to Fructus Anisi Stellati
I.
VerumHook. the adulterant of f..
Illicium (Illicium) the plant whole world has 42 kinds approximately, is mostly to be aiphyllium or shrub, appears spaced apart in East Asia and North America; East Asia is distribution center; And China (on the south the Changjiang river) is the main body at center, has 28 kinds more than (account for the world total 62%), and wherein 19 kinds be Chinese peculiar.The Illicium plant generally all has multiple biological activity, income " Chinese pharmacopoeia Fructus Anisi Stellati and Cortex Illicii arranged.Fructus Anisi Stellati (
I.
VerumHook. f.) having warming middle-JIAO regulates the flow of vital energy the effect of dispersing cold for relieving pain; Cortex Illicii (
I. difengpiB. N. Chang et a1) expelling wind and removing dampness is arranged, the function of promoting the circulation of QI to relieve pain cures mainly rheumatic arthritis, diseases such as lumbar muscle strain; The short column anise (
I.
BrecistylumA. C. Smith) external dispelling the wind and dampness pathogens, traumatic injury.
The composition of Illicium plant mainly contains monoterpene, sesquiterpene and terpene alcohol, terpene ether, sesquialter card lactone and derivant, diterpene and triterpenes, flavonoid, phenylpropyl alcohol alkane and compositions such as lignin, organic acid.Main component of essential oil is α-terpinene, α-eudesmol, sagittol, linalool, pinene, camphene etc.Many scholars have reported that this platymiscium has effects such as antitumor, antibacterial, antiinflammatory.But up to now, do not see that about chemical constituent and the pharmacologically active of Folium illicii Lanceolati systematic study is arranged.
The Quercetin of proposition plant extract such as Zhu Shen silver, the XOD that rutin can suppress liver; Reduction hyperuricemia model mouse blood uric acid level (see for details: Zhu Shen silver etc. prevent and treat the progress [J] of gout medicine. medical Leader; 2006,25 (8): 803-806.).Chinese patent document CN:1320891C discloses a kind of Herba Hyperici Japonici extract and preparation method thereof and the application in preparation treatment hepatitis medicament, and the total amount that contains following five kinds of chemical compounds in this extract is not less than 50%wt: taxifolin-7-rhamnoside, isoquercitrin, Quercitroside, glaucoside and Quercetin.Chinese patent document CN:100406027C discloses a kind of Chinese medicine composition that is used to treat hepatic disease and preparation method thereof; This Chinese medicine composition is from Herba Hyperici Japonici, to extract the effective site that purification obtains; Wherein the content of flavones ingredient is 80-99.5%, and this flavones ingredient is mainly Quercitroside, isoquercitrin, the two xanthone C of Herba Hyperici Japonici and the two xanthone D of Herba Hyperici Japonici.Chinese patent document CN:101991593A discloses the application of a kind of Quercitroside in pharmacy, is included in the application in preparation treatment cardiovascular disease medicine and health product, treatment cancer medicine and health product, antiviral drug, antimicrobial, antiinflammatory medicine, treatment pulmonary fibrosis medicine, treatment diabetes medicine and health product, antioxidant drug and the health product.But the medicine about preventing and treating gouty arthritis with the extract and the Quercitroside of Radix Illicii Lanceolati does not also appear in the newspapers at present.
Summary of the invention
The objective of the invention is provides a kind of Chinese medicine extract of treating gouty arthritis to deficiency of the prior art.
One purpose more of the present invention is that the method for preparing of the Chinese medicine extract of treatment gouty arthritis is provided.
Another purpose of the present invention is that the application of the Chinese medicine extract of treatment gouty arthritis is provided.
The 4th purpose of the present invention is that the application of Quercitroside in preparation treatment gouty arthritis disease medicament is provided.
The 5th purpose of the present invention is that the method for preparing of Quercitroside is provided.
For realizing above-mentioned purpose; The technical scheme that the present invention takes is: a kind of Chinese medicine extract of treating gouty arthritis; Described extract is prepared by crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Cortex Illicii, false Cortex Illicii or Flos Albiziae, and content of total flavone is 20-80% in the described extract.
Described total flavones composition is mainly Quercitroside, Quercetin and Quercetin-4'-O-β-D-polyglycoside.
Described Quercitroside content accounts for the 30-80% of extract, and quercetin content accounts for the 5-15% of extract, and Quercetin-4'-O-β-D-polyglycoside content accounts for the 1.5-15% of extract.
It is anistree plain also to contain caffeic acetate, isorhamnetin and East Asia in the described extract.
For realizing above-mentioned second purpose; The technical scheme that the present invention takes is: a kind of method for preparing of treating the Chinese medicine extract of gouty arthritis; Described method for preparing may further comprise the steps: after crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Cortex Illicii, false Cortex Illicii or Flos Albiziae decoct; Be condensed into extractum; Add extraction etoh solvent or methanol and carry out precipitate with ethanol, the supernatant concentration behind the precipitate with ethanol is crossed macroporous resin column; Collect the 30-70% position, the dry extract that gets of concentrating under reduced pressure.
A kind of method for preparing of treating the Chinese medicine extract of gouty arthritis; Described method for preparing may further comprise the steps: crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Cortex Illicii, false Cortex Illicii or Flos Albiziae are pulverized; To extract solvent 30-95% ethanol or methanol eddy; Filtrating concentrates; Cross macroporous resin column; Collect the 30-70% position, the dry extract that gets of concentrating under reduced pressure.
A kind of method for preparing of treating the Chinese medicine extract of gouty arthritis; Described method for preparing may further comprise the steps: crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Cortex Illicii, false Cortex Illicii or Flos Albiziae are to extract solvent 30-95% ethanol or methanol; Till percolation to flavonoid assay reactions is negative; Collect percolate, get extracting solution; Said extracted liquid is concentrated, and distilled water is regulated proportion to 1.05g/ml, and filtrating is passed through macroporous resin column, and discards effluent; Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, eluent concentrating under reduced pressure drying obtains extract.
Described extraction solvent is a 70-95% ethanol.
Described macroporous resin is selected from AB-8, D-101, ZTC-1 or SP905.
For realizing above-mentioned the 3rd purpose, the technical scheme that the present invention takes is: the application of described extract in preparation treatment gouty arthritis disease medicament.
The application of described extract in preparation treatment rheumatic arthritis or rheumatoid arthritis disease medicament.
The dosage form of described medicine is injectable powder, tablet, capsule, granule, oral liquid, drop pill, pill, injection, unguentum, mixture, patch, small-volume injection or gel.
For realizing above-mentioned the 4th purpose, the technical scheme that the present invention takes is: the application of Quercitroside in preparation treatment gouty arthritis disease medicament, and the structural formula of described Quercitroside is:
The application of Quercitroside in preparation treatment rheumatic arthritis or rheumatoid arthritis disease medicament.
The dosage form of described medicine is injectable powder, tablet, capsule, granule, oral liquid, drop pill, pill, injection, unguentum, mixture, patch, small-volume injection or gel.
For realizing above-mentioned the 5th purpose, the technical scheme that the present invention takes is: described method for preparing may further comprise the steps: described extract gets the effective ingredient Quercitroside through silicagel column, macroporous resin column, polyamide column or gel column eluting.
Described method for preparing may further comprise the steps: crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Radix Illicii Lanceolati peel of stem, Radix Illicii Lanceolati fruit, Cortex Illicii, false Cortex Illicii or Flos Albiziae; With ethanol percolate extraction; Merge percolate; Concentrating under reduced pressure; Add the aquae destillata suspendible; Earlier with petroleum ether extraction; Mother solution is with ethyl acetate extraction; The reclaim under reduced pressure ethyl acetate; With extract dry, get extractum and add equivalent silica gel and mix porphyrize thoroughly; Last silicagel column, macroporous resin column, polyamide column or gel column, eluting get the effective ingredient Quercitroside.
Described method for preparing may further comprise the steps: crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Radix Illicii Lanceolati peel of stem, Radix Illicii Lanceolati fruit, Cortex Illicii, false Cortex Illicii or Flos Albiziae are pulverized; With 30-95% ethanol or methanol eddy; Filtrating is concentrated into 1:1; Behind the petroleum ether extraction; Use ethyl acetate extraction; The extract concentrating under reduced pressure is dry, crosses silicagel column, macroporous resin column, polyamide column or gel column, and eluting gets the effective ingredient Quercitroside.
The invention has the advantages that:
1, the present invention is the extract of primary raw material preparation with the Illicium plant; It has significant preventive and therapeutic effect to acute gouty arthritis through the pharmacological evaluation proof; Have no side effect, can be used in the medicine that preparation prevents and treats gouty arthritis, rheumatic or rheumatoid arthritis;
2, found the new purposes of Quercitroside treatment gouty arthritis; Through method for preparing of the present invention, removed toxic component, and effective ingredient obtains concentrating reservation; Improve application curative effect and safety, be convenient to the safe handling in medical industry and fields such as relevant industries such as food, beverage.
The specific embodiment
Below in conjunction with embodiment the specific embodiment provided by the invention is elaborated.
The preparation (one) of embodiment 1 Chinese medicine extract
Bark of Radix Illicii Lanceolati is cleaned, and soaks to insert after 6-24 hour in the extraction vessel to decoct 3-5 time, and each time that decocts is 0.5-3 hour.Combining medicine with extracting for several times is condensed into extractum.After extractum left standstill, add ethanol and carry out precipitate with ethanol, reclaim ethanol and go deposition.Get the supernatant behind the precipitate with ethanol, concentrate, cross macroporous resin (AB-8) post, collect the 30-70% position, concentrating under reduced pressure dry extract TIL.
Adopt the spectrophotometry content of total flavone.With the Quercetin is reference substance, is developer with the aluminum chloride, and the detection wavelength is 431nm, and recording general flavone content is 20-80%.The HPLC method is measured, Agilent C
18Post; Mobile phase is acetonitrile-0.1% phosphoric acid solution (25:75); Detecting wavelength is 256 nm; Flow velocity is 1.0 mL/min; 25 ℃ of column temperatures; The sample feeding amount is 10uL.Quercitroside content accounts for the 30-80% of extract as a result, and Quercetin accounts for 5-15%, and Quercetin-4'-O-β-D-polyglycoside accounts for 1.5-15%.Contain the anistree element in a spot of caffeic acetate, isorhamnetin and East Asia etc. in addition.
The preparation (two) of embodiment 2 Chinese medicine extract
Radix Illicii Lanceolati or Radix Illicii Lanceolati stem and leaf are cleaned, and soak to insert after 6-24 hour in the extraction vessel to decoct 3-5 time, and each time that decocts is 0.5-3 hour.Combining medicine with extracting for several times is condensed into extractum.After extractum left standstill, add ethanol and carry out precipitate with ethanol, reclaim ethanol and go deposition.Get the supernatant behind the precipitate with ethanol, concentrate, cross macroporous resin (D-101) post, collect the 30-70% position, concentrating under reduced pressure dry extract TIL.
The preparation (three) of embodiment 3 Chinese medicine extract
Bark of Radix Illicii Lanceolati is cleaned, and soaks to insert after 6-24 hour in the extraction vessel to decoct 3-5 time, and each time that decocts is 0.5-3 hour.Combining medicine with extracting for several times is condensed into extractum.After extractum left standstill, add methanol and carry out precipitate with ethanol, reclaim methanol and go deposition.Get the supernatant behind the precipitate with ethanol, concentrate, cross macroporous resin (ZTC-1) post, collect the 30-70% position, concentrating under reduced pressure dry extract TIL.
The preparation (four) of embodiment 4 Chinese medicine extract
Cortex Illicii is cleaned, and soaks to insert after 6-24 hour in the extraction vessel to decoct 3-5 time, and each time that decocts is 0.5-3 hour.Combining medicine with extracting for several times is condensed into extractum.After extractum left standstill, add ethanol and carry out precipitate with ethanol, reclaim ethanol and go deposition.Get the supernatant behind the precipitate with ethanol, concentrate, cross macroporous resin (SP905) post, collect the 30-70% position, concentrating under reduced pressure dry extract TIL.
The preparation (five) of embodiment 5 Chinese medicine extract
False Cortex Illicii is cleaned, and soaks to insert after 6-24 hour in the extraction vessel to decoct 3-5 time, and each time that decocts is 0.5-3 hour.Combining medicine with extracting for several times is condensed into extractum.After extractum left standstill, add ethanol and carry out precipitate with ethanol, reclaim ethanol and go deposition.Get the supernatant behind the precipitate with ethanol, concentrate, cross macroporous resin (AB-8) post, collect the 30-70% position, concentrating under reduced pressure dry extract TIL.
The preparation (six) of embodiment 6 Chinese medicine extract
Flos Albiziae is cleaned, and soaks to insert after 6-24 hour in the extraction vessel to decoct 3-5 time, and each time that decocts is 0.5-3 hour.Combining medicine with extracting for several times is condensed into extractum.After extractum left standstill, add ethanol and carry out precipitate with ethanol, reclaim ethanol and go deposition.Get the supernatant behind the precipitate with ethanol, concentrate, cross macroporous resin (D-101) post, collect the 30-70% position, concentrating under reduced pressure dry extract TIL.
The preparation (seven) of embodiment 7 Chinese medicine extract
The bark of Radix Illicii Lanceolati pulverizing, with the 30-95% alcohol reflux, filtrating concentrates, and crosses macroporous resin (AB-8) post, collects the 30-70% position, the dry extract TIL that gets of concentrating under reduced pressure.Preferred 8 times are extracted solvent during reflux, extract,, reflux each 2 hours 3 times.
Adopt the spectrophotometry content of total flavone.With the Quercetin is reference substance, is developer with the aluminum chloride, and the detection wavelength is 431nm, and recording general flavone content is 20-80%.The HPLC method is measured, Agilent C
18Post; Mobile phase is acetonitrile-0.1% phosphoric acid solution (25:75); Detecting wavelength is 256 nm; Flow velocity is 1.0 mL/min; 25 ℃ of column temperatures; The sample feeding amount is 10uL.Quercitroside content accounts for the 30-80% of extract as a result, and Quercetin accounts for 5-15%, and Quercetin-4'-O-β-D-polyglycoside accounts for 1.5-15%.Contain the anistree element in a spot of caffeic acetate, isorhamnetin and East Asia etc. in addition.
The preparation (eight) of embodiment 8 Chinese medicine extract
The bark of Radix Illicii Lanceolati pulverizing, with the 70-95% alcohol reflux, filtrating concentrates, and crosses macroporous resin (D-101) post, collects the 30-70% position, the dry extract TIL that gets of concentrating under reduced pressure.Preferred 8 times are extracted solvent during reflux, extract,, reflux each 2 hours 3 times.
The preparation (nine) of embodiment 9 Chinese medicine extract
The bark of Radix Illicii Lanceolati pulverizing, with the 30-95% methanol eddy, filtrating concentrates, and crosses macroporous resin (ZTC-1) post, collects the 30-70% position, the dry extract TIL that gets of concentrating under reduced pressure.Preferred 8 times are extracted solvent during reflux, extract,, reflux each 2 hours 3 times.
The preparation (ten) of embodiment 10 Chinese medicine extract
Radix Illicii Lanceolati or the pulverizing of Radix Illicii Lanceolati stem and leaf, with the 30-95% alcohol reflux, filtrating concentrates, and crosses macroporous resin (SP905) post, collects the 30-70% position, the dry extract TIL that gets of concentrating under reduced pressure.Preferred 8 times are extracted solvent during reflux, extract,, reflux each 2 hours 3 times.
The preparation (11) of embodiment 11 Chinese medicine extract
The Cortex Illicii pulverizing, with the 30-95% alcohol reflux, filtrating concentrates, and crosses macroporous resin (AB-8) post, collects the 30-70% position, the dry extract TIL that gets of concentrating under reduced pressure.
The preparation (12) of embodiment 12 Chinese medicine extract
False Cortex Illicii pulverizing, with the 30-95% alcohol reflux, filtrating concentrates, and crosses macroporous resin (AB-8) post, collects the 30-70% position, the dry extract TIL that gets of concentrating under reduced pressure.
The preparation (13) of embodiment 13 Chinese medicine extract
The Flos Albiziae pulverizing, with the 30-95% alcohol reflux, filtrating concentrates, and crosses macroporous resin (AB-8) post, collects the 30-70% position, the dry extract TIL that gets of concentrating under reduced pressure.
The preparation (14) of embodiment 14 Chinese medicine extract
Bark of Radix Illicii Lanceolati is pressed the pharmacopeia method for 5 kilograms and is extracted volatile oil earlier, gets effective site I2.Residue dries, and with 0-95% ethanol, till being negative by conventional percolation to flavonoid assay reactions, collects percolate, extracting solution.Said extracted liquid is concentrated into nothing alcohol flavor; Distilled water is regulated proportion to 1.05g/ml (25 ℃); The macroporous resin that filtrating is crossed through activation processing (AB-8) post (every Kg medical material is with activation wet resin 1Kg) makes the active component in the filtrating adsorbed by resin column, and discards effluent.Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, 60 ℃ of concentrating under reduced pressure dryings of eluent obtain extract TIL.
Adopt the spectrophotometry content of total flavone.With the Quercetin is reference substance, is developer with the aluminum chloride, and the detection wavelength is 431nm, and recording general flavone content is 20-80%.The HPLC method is measured, Agilent C
18Post; Mobile phase is acetonitrile-0.1% phosphoric acid solution (25:75); Detecting wavelength is 256 nm; Flow velocity is 1.0 mL/min; 25 ℃ of column temperatures; The sample feeding amount is 10uL.Quercitroside content accounts for the 30-80% of extract as a result, and Quercetin accounts for 5-15%, and Quercetin-4'-O-β-D-polyglycoside accounts for 1.5-15%.Contain the anistree element in a spot of caffeic acetate, isorhamnetin and East Asia etc. in addition.
With volatile oil I2 and TIL proportional mixing, pharmacological evaluation shows, can strengthen the pharmacological action of anti-inflammatory and antalgic.
The preparation (15) of embodiment 15 Chinese medicine extract
5 kilograms of bark of Radix Illicii Lanceolati are with 30-95% ethanol, till being negative by conventional percolation to flavonoid assay reactions, collect percolate, extracting solution.Said extracted liquid is concentrated into nothing alcohol flavor; Distilled water is regulated proportion to 1.05g/ml (25 ℃); The macroporous resin that filtrating is crossed through activation processing (D-101) post (every Kg medical material is with activation wet resin 1Kg) makes the active component in the filtrating adsorbed by resin column, and discards effluent.Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, 60 ℃ of concentrating under reduced pressure dryings of eluent obtain extract TIL.
The preparation (16) of embodiment 16 Chinese medicine extract
5 kilograms of bark of Radix Illicii Lanceolati are with 70-95% ethanol, till being negative by conventional percolation to flavonoid assay reactions, collect percolate, extracting solution.Said extracted liquid is concentrated into nothing alcohol flavor; Distilled water is regulated proportion to 1.05g/ml (25 ℃); The macroporous resin that filtrating is crossed through activation processing (ZTC-1) post (every Kg medical material is with activation wet resin 1Kg) makes the active component in the filtrating adsorbed by resin column, and discards effluent.Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, 60 ℃ of concentrating under reduced pressure dryings of eluent obtain extract TIL.
The preparation (17) of embodiment 17 Chinese medicine extract
5 kilograms of bark of Radix Illicii Lanceolati are with 70-95% methanol, till being negative by conventional percolation to flavonoid assay reactions, collect percolate, extracting solution.Said extracted liquid is concentrated into nothing alcohol flavor; Distilled water is regulated proportion to 1.05g/ml (25 ℃); The macroporous resin that filtrating is crossed through activation processing (SP905) post (every Kg medical material is with activation wet resin 1Kg) makes the active component in the filtrating adsorbed by resin column, and discards effluent.Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, 60 ℃ of concentrating under reduced pressure dryings of eluent obtain extract TIL.
The preparation (18) of embodiment 18 Chinese medicine extract
5 kilograms of Radix Illicii Lanceolati or Radix Illicii Lanceolati stem and leaf be with 70-95% ethanol, till being negative by conventional percolation to flavonoid assay reactions, collects percolate, extracting solution.Said extracted liquid is concentrated into nothing alcohol flavor; Distilled water is regulated proportion to 1.05g/ml (25 ℃); The macroporous resin that filtrating is crossed through activation processing (AB-8) post (every Kg medical material is with activation wet resin 1Kg) makes the active component in the filtrating adsorbed by resin column, and discards effluent.Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, 60 ℃ of concentrating under reduced pressure dryings of eluent obtain extract TIL.
The preparation (19) of embodiment 19 Chinese medicine extract
Cortex Illicii is with 70-95% ethanol, till being negative by conventional percolation to flavonoid assay reactions, collects percolate, extracting solution.Said extracted liquid is concentrated into nothing alcohol flavor; Distilled water is regulated proportion to 1.05g/ml (25 ℃); The macroporous resin that filtrating is crossed through activation processing (AB-8) post (every Kg medical material is with activation wet resin 1Kg) makes the active component in the filtrating adsorbed by resin column, and discards effluent.Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, 60 ℃ of concentrating under reduced pressure dryings of eluent obtain extract TIL.
The preparation (20) of embodiment 20 Chinese medicine extract
False Cortex Illicii is with 70-95% ethanol, till being negative by conventional percolation to flavonoid assay reactions, collects percolate, extracting solution.Said extracted liquid is concentrated into nothing alcohol flavor; Distilled water is regulated proportion to 1.05g/ml (25 ℃); The macroporous resin that filtrating is crossed through activation processing (AB-8) post (every Kg medical material is with activation wet resin 1Kg) makes the active component in the filtrating adsorbed by resin column, and discards effluent.Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, 60 ℃ of concentrating under reduced pressure dryings of eluent obtain extract TIL.
The preparation (21) of embodiment 21 Chinese medicine extract
Flos Albiziae is with 70-95% ethanol, till being negative by conventional percolation to flavonoid assay reactions, collects percolate, extracting solution.Said extracted liquid is concentrated into nothing alcohol flavor; Distilled water is regulated proportion to 1.05g/ml (25 ℃); The macroporous resin that filtrating is crossed through activation processing (AB-8) post (every Kg medical material is with activation wet resin 1Kg) makes the active component in the filtrating adsorbed by resin column, and discards effluent.Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, 60 ℃ of concentrating under reduced pressure dryings of eluent obtain extract TIL.
The preparation (one) of embodiment 22 effective ingredient Quercitrosides (IL-A)
5 kilograms of bark of Radix Illicii Lanceolati, 10 times of amount 75% ethanol percolate extraction merge percolate; Concentrating under reduced pressure adds the aquae destillata suspendible to there not being the alcohol flavor, earlier with 5 times of amount petroleum ether (60-90 degree) extraction three times; Mother solution is measured ethyl acetate extractions 3 times with 5 times, and the reclaim under reduced pressure ethyl acetate is with extract dry; Get extractum 40 grams; Add equivalent silica gel and mix thoroughly, porphyrize, last silicagel column; With dichloromethane: methanol 30:1 eluting; Collect the common 4500ml of eluent, back with dichloromethane: methanol 20:1 eluting, collect flow point 2000ml; With dichloromethane: methanol 15:1 eluting 3000ml; With dichloromethane: methanol 10:1 eluting 3000ml, with dichloromethane: methanol 5:1 eluting, every 500ml collects one section; Be total to S1-S10 3000ml; S3-S7 merges, and concentrates, and places; Separate out crystallization, obtain IL-A 2500mg.
IL-A: be yellow crystal (methanol) mp 181-182 ℃; 1H-NMR (600 MHz, CD
3OD) δ: 7.32 (1H, d, J=1.9 Hz, H-2 '), 7.28 (1H; Dd, J=8.2,1.9 Hz, H-6 '), 6.90 (1H; D, J=8.2 Hz, H-5 '), 6.33 (1H, d; J=1.8 Hz, H-8), 6.17 (1H, d, J=1.8 Hz; H-6), 5.33 (1H, d, J=2.0 Hz, H-1 "); 4.22-3.29 (sugar H), 0.93 (3H, d, J=6.0Hz, H-6 ").13C-NMR?(600?MHz,?CD
3OD)?δ:?158.5?(C-2),?136.2?(C-3),?179.6?(C-4),?159.3?(C-5),?99.7?(C-6),?165.8?(C-7),?94.7?(C-8),?163.1?(C-9),?105.9?(C-10),?122.9?(C-1′),?116.3?(C-2′),?146.3?(C-3′),?149.7?(C-4′),?116.9?(C-5′),?122.9?(C-6′),?103.5?(C-1′′),?72.0?(C-2′′),?72.1?(C-3′′),?73.2?(C-4′′),?71.9?(C-5′′),?17.6?(C-6′′)。By above spectroscopic data and physicochemical property identify this chemical compound be Quercitroside (
Quercitrin).Purity is more than 95%.
The preparation (two) of embodiment 23 effective ingredient Quercitrosides (IL-A)
5 kilograms of Radix Illicii Lanceolatis, 10 times of amount 75% ethanol percolate extraction merge percolate; Concentrating under reduced pressure adds the aquae destillata suspendible to there not being the alcohol flavor, earlier with 5 times of amount petroleum ether (60-90 degree) extraction three times; Mother solution is measured ethyl acetate extractions 3 times with 5 times, and the reclaim under reduced pressure ethyl acetate is with extract dry; Get extractum 40 grams; Add equivalent silica gel and mix thoroughly, porphyrize, last macroporous resin column; With dichloromethane: methanol 30:1 eluting; Collect the common 4500ml of eluent, back with dichloromethane: methanol 20:1 eluting, collect flow point 2000ml; With dichloromethane: methanol 15:1 eluting 3000ml; With dichloromethane: methanol 10:1 eluting 3000ml, with dichloromethane: methanol 5:1 eluting, every 500ml collects one section; Be total to S1-S10 3000ml; S3-S7 merges, and concentrates, and places; Separate out crystallization, obtain IL-A.
The preparation (three) of embodiment 24 effective ingredient Quercitrosides (IL-A)
The Radix Illicii Lanceolati stem and leaf, 10 times of amount 75% ethanol percolate extraction merge percolate; Concentrating under reduced pressure adds the aquae destillata suspendible to there not being the alcohol flavor, earlier with 5 times of amount petroleum ether (60-90 degree) extraction three times; Mother solution is measured ethyl acetate extractions 3 times with 5 times, and the reclaim under reduced pressure ethyl acetate is with extract dry; Get extractum 40 grams; Add equivalent silica gel and mix thoroughly, porphyrize, last polyamide column; With dichloromethane: methanol 30:1 eluting; Collect the common 4500ml of eluent, back with dichloromethane: methanol 20:1 eluting, collect flow point 2000ml; With dichloromethane: methanol 15:1 eluting 3000ml; With dichloromethane: methanol 10:1 eluting 3000ml, with dichloromethane: methanol 5:1 eluting, every 500ml collects one section; Be total to S1-S10 3000ml; S3-S7 merges, and concentrates, and places; Separate out crystallization, obtain IL-A.
The preparation (four) of embodiment 25 effective ingredient Quercitrosides (IL-A)
The Radix Illicii Lanceolati peel of stem, 10 times of amount 75% ethanol percolate extraction merge percolate; Concentrating under reduced pressure adds the aquae destillata suspendible to there not being the alcohol flavor, earlier with 5 times of amount petroleum ether (60-90 degree) extraction three times; Mother solution is measured ethyl acetate extractions 3 times with 5 times, and the reclaim under reduced pressure ethyl acetate is with extract dry; Get extractum 40 grams; Add equivalent silica gel and mix thoroughly, porphyrize, last gel column (Sephadex LH-20 post); With dichloromethane: methanol 30:1 eluting; Collect the common 4500ml of eluent, back with dichloromethane: methanol 20:1 eluting, collect flow point 2000ml; With dichloromethane: methanol 15:1 eluting 3000ml; With dichloromethane: methanol 10:1 eluting 3000ml, with dichloromethane: methanol 5:1 eluting, every 500ml collects one section; Be total to S1-S10 3000ml; S3-S7 merges, and concentrates, and places; Separate out crystallization, obtain IL-A.
The preparation (five) of embodiment 26 effective ingredient Quercitrosides (IL-A)
Flos Albiziae, 10 times of amount 75% ethanol percolate extraction merge percolate; Concentrating under reduced pressure adds the aquae destillata suspendible to there not being the alcohol flavor, earlier with 5 times of amount petroleum ether (60-90 degree) extraction three times; Mother solution is measured ethyl acetate extractions 3 times with 5 times, and the reclaim under reduced pressure ethyl acetate is with extract dry; Get extractum 40 grams; Add equivalent silica gel and mix thoroughly, porphyrize, last silicagel column; With dichloromethane: methanol 30:1 eluting; Collect the common 4500ml of eluent, back with dichloromethane: methanol 20:1 eluting, collect flow point 2000ml; With dichloromethane: methanol 15:1 eluting 3000ml; With dichloromethane: methanol 10:1 eluting 3000ml, with dichloromethane: methanol 5:1 eluting, every 500ml collects one section; Be total to S1-S10 3000ml; S3-S7 merges, and concentrates, and places; Separate out crystallization, obtain IL-A.Yield is at 0.8-3.5%.
The preparation (six) of embodiment 27 effective ingredient Quercitrosides (IL-A)
Cortex Illicii, 10 times of amount 75% ethanol percolate extraction merge percolate; Concentrating under reduced pressure adds the aquae destillata suspendible to there not being the alcohol flavor, earlier with 5 times of amount petroleum ether (60-90 degree) extraction three times; Mother solution is measured ethyl acetate extractions 3 times with 5 times, and the reclaim under reduced pressure ethyl acetate is with extract dry; Get extractum 40 grams; Add equivalent silica gel and mix thoroughly, porphyrize, last silicagel column; With dichloromethane: methanol 30:1 eluting; Collect the common 4500ml of eluent, back with dichloromethane: methanol 20:1 eluting, collect flow point 2000ml; With dichloromethane: methanol 15:1 eluting 3000ml; With dichloromethane: methanol 10:1 eluting 3000ml, with dichloromethane: methanol 5:1 eluting, every 500ml collects one section; Be total to S1-S10 3000ml; S3-S7 merges, and concentrates, and places; Separate out crystallization, obtain IL-A.
The preparation (seven) of embodiment 28 effective ingredient Quercitrosides (IL-A)
False Cortex Illicii, 10 times of amount 75% ethanol percolate extraction merge percolate; Concentrating under reduced pressure adds the aquae destillata suspendible to there not being the alcohol flavor, earlier with 5 times of amount petroleum ether (60-90 degree) extraction three times; Mother solution is measured ethyl acetate extractions 3 times with 5 times, and the reclaim under reduced pressure ethyl acetate is with extract dry; Get extractum 40 grams; Add equivalent silica gel and mix thoroughly, porphyrize, last silicagel column; With dichloromethane: methanol 30:1 eluting; Collect the common 4500ml of eluent, back with dichloromethane: methanol 20:1 eluting, collect flow point 2000ml; With dichloromethane: methanol 15:1 eluting 3000ml; With dichloromethane: methanol 10:1 eluting 3000ml, with dichloromethane: methanol 5:1 eluting, every 500ml collects one section; Be total to S1-S10 3000ml; S3-S7 merges, and concentrates, and places; Separate out crystallization, obtain IL-A.
The preparation (eight) of embodiment 29 effective ingredient Quercitrosides (IL-A)
The extract TIL that the arbitrary embodiment of embodiment 1-21 prepares further separates (separation process is with reference to embodiment 22) through silicagel column, obtains effective ingredient IL-A.
The preparation (nine) of embodiment 30 effective ingredient Quercitrosides (IL-A)
The extract TIL that the arbitrary embodiment of embodiment 1-21 prepares further separates (separation process is with reference to embodiment 23) through macroporous resin column, obtains effective ingredient IL-A.
The preparation (ten) of embodiment 31 effective ingredient Quercitrosides (IL-A)
The extract TIL that the arbitrary embodiment of embodiment 1-21 prepares further separates (separation process is with reference to embodiment 24) through polyamide column, obtains effective ingredient IL-A.
The preparation (11) of embodiment 32 effective ingredient Quercitrosides (IL-A)
The extract TIL that the arbitrary embodiment of embodiment 1-21 prepares further separates (separation process is with reference to embodiment 25) through gel column (Sephadex LH-20 post), obtains effective ingredient IL-A.
The preparation (12) of embodiment 33 effective ingredient Quercitrosides (IL-A)
Bark of Radix Illicii Lanceolati is pulverized, and with the 30-95% alcohol reflux, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.
The preparation (13) of embodiment 34 effective ingredient Quercitrosides (IL-A)
Bark of Radix Illicii Lanceolati is pulverized, and with the 70-95% alcohol reflux, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.
The preparation (14) of embodiment 35 effective ingredient Quercitrosides (IL-A)
Bark of Radix Illicii Lanceolati is pulverized, and with the 70-95% methanol eddy, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.
The preparation (15) of embodiment 36 effective ingredient Quercitrosides (IL-A)
Radix Illicii Lanceolati is pulverized, and with the 30-95% alcohol reflux, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.
The preparation (16) of embodiment 37 effective ingredient Quercitrosides (IL-A)
The Radix Illicii Lanceolati stem and leaf is pulverized, and with the 30-95% alcohol reflux, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.
The preparation (17) of embodiment 38 effective ingredient Quercitrosides (IL-A)
The Radix Illicii Lanceolati peel of stem is pulverized, and with the 30-95% alcohol reflux, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.
The preparation (18) of embodiment 39 effective ingredient Quercitrosides (IL-A)
The Radix Illicii Lanceolati fruit is pulverized, and with the 30-95% alcohol reflux, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.Yield is 0.5-3.5%.
Need to prove that the Radix Illicii Lanceolati fruit has certain toxicity, can not be used to prepare extract TIL, but can be used for the extraction of effective ingredient Quercitroside, through method for preparing of the present invention, toxic component is able to remove, and the effective ingredient Quercitroside obtains concentrating reservation.
The preparation (19) of embodiment 40 effective ingredient Quercitrosides (IL-A)
Cortex Illicii is pulverized, and with the 30-95% alcohol reflux, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.
The preparation (20) of embodiment 41 effective ingredient Quercitrosides (IL-A)
False Cortex Illicii is pulverized, and with the 30-95% alcohol reflux, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.
The preparation (21) of embodiment 42 effective ingredient Quercitrosides (IL-A)
Flos Albiziae is pulverized, and with the 30-95% alcohol reflux, filtrating is concentrated into 1:1, behind the petroleum ether extraction, uses ethyl acetate extraction, and the extract concentrating under reduced pressure is dry, crosses silicagel column, and eluting gets active component IL-A.
Embodiment 43 anti-inflammatory activities-
The mice auricle swelling method
1. laboratory animal
Laboratory animal: the ICR male mice, body weight 18-22g, Si Laike experiments experiment animal Co., Ltd provides by Shanghai; The animal feeding condition is: 21 ± 1 ℃ of room temperatures, and under 12 hours normal round the clock alternative environment, can free pickuping food and water.Test fasting in preceding 15 hours and can't help water.
2. test sample
Extract TIL 50,100,200mg/kg, IL-A (Quercitroside) 25,50,100 mg/kg face with preceding and are made into suspension with 0.5% CMC-Na aqueous solution, shake up.
Positive control: dexamethasone acetate (Shanghai Pharmaceutical's letter friendship pharmacy head factory, lot number 090501), every contains dexamethasone acetate 0.75 mg, dosage 5 mg/kg.Face with preceding and be made into the 0.5mg/ml suspension with 0.5% CMC-Na aqueous solution.
Blank: get equivalent 0.5% CMC-Na aqueous solution and supply examination.Chemistry proinflammatory agent: xylene, AR level, Chemical Reagent Co., Ltd., Sinopharm Group, lot number F200900303
3. experimental procedure
Mice is pressed the body weight random packet, 10 every group, 60 min gastric infusions, 0.2 ml/20g before using the caused by dimethylbenzene xylene inflammation, 20 μ l drip in the Mus auris dextra with xylene, left ear contrast.Behind the 90min dislocation of mice cervical vertebra is caused death, cut two ears along the auricle baseline, the use diameter is that the card punch of 7 mm is laid auricle at the same position of left and right two ears respectively, weighs.
Difference with left and right sides ear weight is its swelling degree:
Swelling percentage rate=(swelling degree/left auricle is heavy) * l00%
Be subjected to examination group suppression ratio={ (blank control group swelling degree mean-be subjected to examination group swelling degree mean)/blank control group swelling degree mean } * l00%.
Each organizes the swelling degree and blank control group compares, t check between the work group.
4. experimental result
See table
1Extract TIL and monomer component IL-A have significant anti-inflammatory activity, and wherein high dose group has the anti-inflammatory activity of highly significant, and suppression ratio is respectively 59.5%, 59.2%.
Table 1? TIL? And IL-A-xylene induced ear swelling degree of influence (
± SD, n = 10)
| Group | Dosage (mgkg -1) | Swelling degree (mg) | Suppression ratio/% |
| Blank | ? | 11.32±4.98 | ? |
| TIL is low | 50 | 7.18±2.61* | 36.6% |
| Among the TIL | 100 | 5.71±1.50* | 49.6% |
| TIL is high | 200 | 4.59±2.64** | 59.5% |
| IL-A is low | 25 | 7.91±2.31* | 30.1% |
| Among the IL-A | 50 | 6.12±1.18** | 45.9% |
| IL-A is high | 100 | 4.62±1.34** | 59.2% |
| Dexamethasone | 5 | 4.47±0.98** | 60.5% |
Compare * with blank control group
P<0.05, * *
P<0.01.
Embodiment 44
Analgesic activities-acetic acid twisting method
1. laboratory animal
The ICR mice, male and female half and half, body weight 18-22g, Si Laike experiments experiment animal Co., Ltd provides by Shanghai; The animal feeding condition is: 21 ± 1 ℃, 12 hours normal round the clock alternative environment of room temperature, animal can free pickuping food and water, test fasting in preceding 15 hours and can't help water.
2. test sample
Extract TIL 50,100,200mg/kg, IL-A (Quercitroside) 25,50,100 mg/kg face with preceding and are made into suspension with 0.5% CMC-Na aqueous solution, shake up.
3. positive control
Indometacin enteric-coated tablet (the Shanghai moral wins pharmaceutical Co. Ltd of scientific and technological group (Anqing), lot number H34021829), every contains indomethacin 25mg, dosage 10mg/kg.Prepare the suspension that becomes 0.4 mg/ml with 0.5% CMC-Na before using.Cold preservation, subsequent use.
4. blank
Get equivalent 0.5% CMC-Na aqueous solution and supply examination.Chemistry algogen: acetic acid (glacial acetic acid), AR level, Chemical Reagent Co., Ltd., Sinopharm Group, lot number 20090322.
5. experimental procedure
ICR mice male and female half and half, by the body weight random packet, 10 every group.Be divided into blank, positive control, total extract high dose group, the total extract low dose group.1h gastric infusion before the modeling, 0.5 ml/20g.1h modeling behind the filling stomach, lumbar injection concentration is the acetum of 0.5-0.6%, 0.2 ml/20g.That writes down mice in 15 minutes turns round the body number of times.Calculate suppression ratio.
Suppression ratio %={ (blank control group turn round body mean-be subjected to the reagent group to turn round the body number of times)/blank control group is turned round the body number of times } * 100%.
6. experimental result
See that table 2 extract TIL and monomer component IL-A have significant analgesic activities, wherein high dose group has the anti-inflammatory activity of highly significant, and suppression ratio is respectively 52.3%, 58.9%.
Table 2 TIL and IL-A pair of acetic acid induced writhing effects (
± SD, n = 10)
| Divide into groups | Dosage (mgkg -1) | Turn round the body number of times | Suppression ratio/% |
| Blank | ? | 30.4 ± 4.72 | ? |
| Indomethacin | 10 | 11.1 ± 2.77** | 63.5% |
| TIL is low | 50 | 23.3± 4.47 | 23.4% |
| Among the TIL | 100 | 19. 3± 2.11* | 36.5% |
| TIL is high | 200 | 14. 5± 2.35** | 52.3% |
| IL-A is low | 25 | 18.8± 3.02* | 38.2% |
| Among the IL-A | 50 | 16.7± 2.14* | 45.1% |
| IL-A is high | 100 | 12.5 ± 2.04** | 58.9% |
Compare with blank control group, * P 0.05, * * P < 0.01.
Embodiment 45 TIL, IL-A cause the influence of acute gouty arthritis rat swollen joint expansibility to uric acid sodium crystallization (MSU)
1. laboratory animal
Male Wistar rat, body weight 170-200g.
2. experimental technique
Rat is divided into 6 groups (n=10) at random: normal group; Model group; TIL high and low dose group (50,200mg/kg) and IL-A high and low dose group (25,100mg/kg) and colchicine (1 mg/kg); The ig administration; Every day 1 time, continuous 3d, lh after the last administration; Normal rats right side ankle joint dorsal part injection physiological saline solution 0.2ml, other each group is injected 0.2 ml MSU suspension in the right ankle joint of rat chamber.Before modeling, 6 h, 12 h, 24h measure the volume of right ankle joint after the modeling, calculate the swollen joint expansibility, the swollen joint expansibility=preceding joint of modeling posterior joint volume-modeling voluminosity, and calculate inhibitory rate of intumesce.Press the method for Coderre etc. behind modeling 24 h and observe the rat gait, 0 grade: normal walking; 1 grade: the slight limping, it is slightly crooked to be tried lower limb; 2 grades: moderate is walked lamely, and is tried lower limb and cuts to pieces and touch ground; 3 grades: severe is walked lamely, and is tried the lower limb built on stilts, the tripodia walking that lands.
3. experimental result
See table 3, compare with normal group, the model group rat injection back obvious swelling in 6h joint, 12h swelling peaks.Compare with model group, indomethacin group, TIL, IL-A high dose group 6-24h rat swollen joint expansibility all obviously reduce, and have significant difference.Compare with normal group, the model group activities in rats reduces, and right hind is crooked, and hind leg lifts during the walking that has, have quiet the time hind leg lift.Compare with model group, colchicine group, TIL high dose group rat gait take an evident turn for the better.
Table 3 TIL, IL-A pair of MSU acute gouty arthritis rats degree of joint swelling (
± SD, n = 10)
Compare with model group, * P 0.05, * * P < 0.01.
Embodiment 46 TIL and IL-A are to the active influence of xanthine oxidase (XOD)
Xanthine oxidase (XO) catalysis hypoxanthine generates xanthine, and further oxidation generates uric acid, in the gout pathogenic process, plays an important role.Xanthine oxidase inhibitor can reduce uricopoiesis, has the effect of prevention gouty arthritis.
1. reagent
Xanthine oxidase (XOD) is measured test kit, builds up bio-engineering research institute available from Nanjing.
2. experimental technique
Experimental implementation is measured the test kit description with reference to xanthine oxidase (XOD) and is carried out.The 530nm place measures absorbance.The mensuration pipe absorbance meansigma methods (deduct blank pipe absorbance meansigma methods) of A value for not adding medicine.The mensuration pipe absorbance meansigma methods (deduct blank pipe absorbance meansigma methods) of B value for adding medicine.The active inhibition of XOD percentage rate=(1-B/A) * 100%, calculate variable concentrations TIL and IL-A to the active percentage rate that suppresses of XOD.And employing computed in software IC
50
3. experimental result
The allopurinol of variable concentrations and TIL and IL-A increase with concentration, and its inhibitory action to xanthine oxidase activity also strengthens.Allopurinol IC
50Be 0.0475 μ g/ml; The IC of TIL and IL-A
50Be respectively 12.4525 μ g/ml, 0.4525 μ g/ml.
Embodiment 47 acute toxicity tests
1. experimental technique
Prepare extract TIL and IL-A by preceding method.Suspension with 0.5% CMC-Na preparation becoming 0.1g/ml.Kunming mouse, 6 ages in week, male and female half and half.Overnight fasting (can't help water).The back weighed in second day by 0.4ml/10g body weight gastric infusion, press above dosage repeat administration once behind the 4h.Each dosage is: 4g/kg/ day, the secondary dosage reaches 8g/kg/ day.Tight observation animal performance after the administration, continuous 7 days.
2. experimental result
The result shows, the equal no abnormality seen of experimental mice each side does not have dead.Body weight gain is good, by increasing to 27.1 ± 1.2g for preceding 20.8 ± 0.56g.Chinese medicine extract gives mice by maximum dosage-feeding, 8g/kg/ day, be equivalent to crude drug amount 320g/kg/ day, and the serious toxicity reaction does not appear in animal.This dosage is clinical people's consumption 9g (crude drug)/60kg/ day) more than 2133 times.Prove that extract of the present invention and IL-A are safe.Bibliographical information lanceolata Fructus Foeniculi has certain toxicity, and through our method for preparing, toxic component is able to remove, and effective ingredient obtains concentrating reservation.Therefore application curative effect and safety have been improved.
Extract TIL of the present invention and IL-1 owing to have the anti-inflammatory and antalgic isoreactivity, therefore also have effect to rheumatic, rheumatoid arthritis except that to the gouty arthritis.Therefore have rheumatism, rheumatoid purposes.
The preparation of embodiment 47 extract TIL and effective ingredient Quercitroside (IL-A) preparation
The present invention prepares injectable powder and generally adopts conventional freeze-drying, as solvent, the steps include: to get TIL or IL-A with water, adds excipient, is dissolved in water, and adds active carbon, filtration sterilization, and plug is partly rolled in fill, and lyophilization, tamponade are rolled lid and are got final product.Used excipient is selected from one or more in mannitol, gelatin hydrolysate, glucose, lactose or the dextran etc.Every bottle contains this Chinese medicine extract 5-50mg.
The present invention prepares injectable powder also can adopt spray drying method, as solvent, the steps include: to get TIL or IL-A with water, adds or do not add excipient, and used excipient is selected from one or more in mannitol, gelatin hydrolysate, glucose, lactose or the dextran etc.Be dissolved in water, add active carbon, filtration sterilization, spray drying, aseptic subpackaged, tamponade is rolled lid and is got final product.Every bottle contains this Chinese medicine extract 5-50mg.
When the present invention prepared small-volume injection, preparation got final product as solvent with water for injection, also can add appropriate amount of auxiliary materials, and adjuvant is selected from one or more in ethanol, propylene glycol, glycerol, Polyethylene Glycol, benzyl benzoate or the dimethyl acetylamide etc.Every contains this Chinese medicine extract 2-50mg.
The present invention prepares oral formulations such as tablet, capsule, granule, oral liquid, and adjuvant can be lactose, starch, dextrin, stearate etc., presses the routine techniques preparation.
One, the preparation of TIL and IL-A injectable powder
Get TIL or IL-A10g, add excipient 10g, add 200ml water for injection, stir and make its dissolving; Add the injection water to 2000ml, add the 1.0g needle-use activated carbon, fully stirred 30 minutes; Decarbonization filtering; With 0.22 μ m filtering with microporous membrane; Be filled in the aseptic cillin bottle, every bottle of 2ml partly rolls plug; Lyophilization, tamponade is rolled lid and is got final product again.
Described excipient is selected from one or more in mannitol, gelatin hydrolysate, glucose, lactose or the dextran etc.
Two, the preparation of TIL and IL-A injectable powder
Get TIL or IL-A10g, add excipient 10g (or not adding excipient), add 200ml water for injection, stir and make its dissolving; Add the injection water to 2000ml, add the 1.0g needle-use activated carbon, fully stirred 30 minutes; Decarbonization filtering; With 0.22 μ m filtering with microporous membrane; Spray drying; Be filled in the aseptic cillin bottle, every bottle of 2ml, tamponade is rolled lid and is got final product again.
Described excipient is selected from one or more in mannitol, gelatin hydrolysate, glucose, lactose or the dextran etc.
Three, the preparation of TIL and IL-A small-volume injection
Get TIL or IL-A 1g, add 100ml water for injection, stir and make its dissolving; Add the injection water to 1000ml, with 0.22 μ m filtering with microporous membrane; The packing embedding, every bottle of 2ml, sterilization gets final product.
Can add appropriate amount of auxiliary materials, adjuvant is selected from one or more in ethanol, propylene glycol, glycerol, Polyethylene Glycol, benzyl benzoate or the dimethyl acetylamide etc.
Four, the preparation of TIL and IL-A granule
Get TIL or IL-A and add the adjuvant granulation, drying is crossed 300 mesh sieves.Be packaged into the 10g/ bag, finished particle.Described adjuvant can be lactose, starch, dextrin, stearate etc.
Five, the preparation of TIL and IL-A tablet
Get TIL or IL-A50g, starch 40g, lactose 30g; Microcrystalline Cellulose 30g, 7% starch slurry is an amount of, magnesium stearate 1%; By said ratio extract powder, starch, lactose, microcrystalline Cellulose are crossed 80 mesh sieves respectively, mixing is after 40 mesh sieves 3 times; Above-mentioned mixed powder with 7% starch slurry system soft material, is crossed 24 mesh sieve grains, 50 ℃ of dryings 2 hours; Dried particles is crossed 30 mesh sieve granulate, adds magnesium stearate, mixing; Tabletting gets 1000 in tablet.
Six, the preparation of TIL and IL-A gel
Extract TIL or IL-1 also can be made into external preparation.Preceding legal system is equipped with Chinese medicine extract TIL or IL-1, adds 1-3 part propylene glycol or glycerol, and 1-3 part tragakanta or gelatin or sodium carboxymethyl cellulose, and 0.5-1 part laurocapram are prepared into gel.
TIL 10g, carbomer 10g, glycerol or propylene glycol 10g, the tall and erect azone 10g of Laurel, triethanolamine 5ml.1. get TIL, glycerol and add in an amount of distilled water stirring and dissolving successively.2. the heat-obtaining distilled water is an amount of, pours carbomer into, and stir make its natural swelling after, add the tall and erect azone of Laurel, stir.In pouring into this solution 1., stir.Add triethanolamine again and fully stir, the reuse distilled water adds to 1000g, stirs, and promptly gets gel, and packing gets final product.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the inventive method; Can also make some improvement and replenish, these improvement and replenish and also should be considered as protection scope of the present invention.
Claims (18)
1. Chinese medicine extract of treating gouty arthritis; It is characterized in that; Described extract is prepared by crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Cortex Illicii, false Cortex Illicii or Flos Albiziae, and content of total flavone is 20-80% in the described extract.
2. extract according to claim 1 is characterized in that, described total flavones composition is mainly Quercitroside, Quercetin and Quercetin-4'-O-β-D-polyglycoside.
3. extract according to claim 3 is characterized in that described Quercitroside content accounts for the 30-80% of extract, and quercetin content accounts for the 5-15% of extract, and Quercetin-4'-O-β-D-polyglycoside content accounts for the 1.5-15% of extract.
4. extract according to claim 3 is characterized in that, it is anistree plain also to contain caffeic acetate, isorhamnetin and East Asia in the described extract.
5. method for preparing of treating the Chinese medicine extract of gouty arthritis; It is characterized in that; Described method for preparing may further comprise the steps: after crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Cortex Illicii, false Cortex Illicii or Flos Albiziae decoct; Be condensed into extractum; Add extraction etoh solvent or methanol and carry out precipitate with ethanol, the supernatant concentration behind the precipitate with ethanol is crossed macroporous resin column; Collect the 30-70% position, the dry extract that gets of concentrating under reduced pressure.
6. method for preparing of treating the Chinese medicine extract of gouty arthritis; It is characterized in that; Described method for preparing may further comprise the steps: crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Cortex Illicii, false Cortex Illicii or Flos Albiziae are pulverized; To extract solvent 30-95% ethanol or methanol eddy; Filtrating concentrates; Cross macroporous resin column, collect the 30-70% position, the dry extract that gets of concentrating under reduced pressure.
7. method for preparing of treating the Chinese medicine extract of gouty arthritis; It is characterized in that; Described method for preparing may further comprise the steps: crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Cortex Illicii, false Cortex Illicii or Flos Albiziae are to extract solvent 30-95% ethanol or methanol; Till percolation to flavonoid assay reactions is negative; Collect percolate, get extracting solution; Said extracted liquid is concentrated, and distilled water is regulated proportion to 1.05g/ml, and filtrating is passed through macroporous resin column, and discards effluent; Use the water elution macroporous resin column, discard eluent; Continue with 30-70% ethanol elution macroporous resin column, collect 30-70% position eluent, eluent concentrating under reduced pressure drying obtains extract.
8. according to the method for preparing of the arbitrary described Chinese medicine extract of claim 5-7, it is characterized in that described extraction solvent is a 70-95% ethanol.
9. according to the method for preparing of the arbitrary described Chinese medicine extract of claim 5-7, it is characterized in that described macroporous resin is selected from AB-8, D-101, ZTC-1 or SP905.
10. according to the application of the arbitrary described extract of claim 1-4 in preparation treatment gouty arthritis disease medicament.
11. according to the application of the arbitrary described extract of claim 1-4 in preparation treatment rheumatic arthritis or rheumatoid arthritis disease medicament.
12., it is characterized in that the dosage form of described medicine is injectable powder, tablet, capsule, granule, oral liquid, drop pill, pill, injection, unguentum, mixture, patch, small-volume injection or gel according to claim 10 or 11 arbitrary described application.
13. the application of Quercitroside in preparation treatment gouty arthritis disease medicament is characterized in that the structural formula of described Quercitroside is:
14. the application of Quercitroside in preparation treatment rheumatic arthritis or rheumatoid arthritis disease medicament.
15., it is characterized in that the dosage form of described medicine is injectable powder, tablet, capsule, granule, oral liquid, drop pill, pill, injection, unguentum, mixture, patch, small-volume injection or gel according to claim 13 or 14 arbitrary described application.
16. method for preparing according to claim 13 or 14 arbitrary described Quercitrosides; It is characterized in that; Described method for preparing may further comprise the steps: with the arbitrary described extract of claim 1-4, get the effective ingredient Quercitroside through silicagel column, macroporous resin column, polyamide column or gel column eluting.
17. method for preparing according to claim 13 or 14 arbitrary described Quercitrosides; It is characterized in that; Described method for preparing may further comprise the steps: the crude drug bark of Radix Illicii Lanceolati; Radix Illicii Lanceolati; The Radix Illicii Lanceolati stem and leaf; The Radix Illicii Lanceolati peel of stem; The Radix Illicii Lanceolati fruit; Cortex Illicii; False Cortex Illicii or Flos Albiziae; With ethanol percolate extraction; Merge percolate; Concentrating under reduced pressure; Add the aquae destillata suspendible, earlier with petroleum ether extraction, mother solution is with ethyl acetate extraction; The reclaim under reduced pressure ethyl acetate; With extract dry, get extractum and add equivalent silica gel and mix porphyrize thoroughly; Last silicagel column; Macroporous resin column; Polyamide column or gel column, eluting get the effective ingredient Quercitroside.
18. method for preparing according to claim 13 or 14 arbitrary described Quercitrosides; It is characterized in that; Described method for preparing may further comprise the steps: crude drug bark of Radix Illicii Lanceolati, Radix Illicii Lanceolati, Radix Illicii Lanceolati stem and leaf, Radix Illicii Lanceolati peel of stem, Radix Illicii Lanceolati fruit, Cortex Illicii, false Cortex Illicii or Flos Albiziae are pulverized; With 30-95% ethanol or methanol eddy; Filtrating is concentrated into 1:1; Behind the petroleum ether extraction; Use ethyl acetate extraction; The extract concentrating under reduced pressure is dry; Cross silicagel column, macroporous resin column, polyamide column or gel column, eluting gets the effective ingredient Quercitroside.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102688294A (en) * | 2012-06-13 | 2012-09-26 | 浙江农林大学 | Illicium henryi-contained anti-virus ointment for external use and preparation method and application thereof |
| CN102935133A (en) * | 2012-12-08 | 2013-02-20 | 南京正宽医药科技有限公司 | Traditional Chinese medicine composition for treating rheumatic arthritis as well as preparation method and application thereof |
| CN104586937A (en) * | 2013-11-01 | 2015-05-06 | 浙江泰康药业集团有限公司 | Preparation method of illicium plants injection |
| CN105816524A (en) * | 2016-04-18 | 2016-08-03 | 广西壮族自治区中国科学院广西植物研究所 | Anti-inflammatory active cortex illicii extract as well as preparation method and application thereof |
| CN106389597A (en) * | 2016-11-24 | 2017-02-15 | 广州和匠科技有限公司 | Method for efficiently extracting edible and medicinal monomers preventing and treating hyperuricemia and gout from coffee |
| CN106728137A (en) * | 2016-11-24 | 2017-05-31 | 广州和匠科技有限公司 | It is a kind of that the preparation method for preventing and treating hyperuricemia and gout medicine-food two-purpose monomer is extracted from coffee |
| CN106974921A (en) * | 2016-01-15 | 2017-07-25 | 滨州医学院 | 2R-cardiospermin-5-p-hydroxybenzoate preparation and its application in drugs for rheumatoid arthritis is prepared |
| CN108295052A (en) * | 2017-12-28 | 2018-07-20 | 中国人民解放军第二军医大学 | The application of I. henry Diels glycosides ether and composition in urarthritis |
| CN108392509A (en) * | 2018-05-18 | 2018-08-14 | 华中农业大学 | A kind of Pollen Brassicae campestris extract and application thereof |
| CN112089692A (en) * | 2020-09-10 | 2020-12-18 | 浙江赛沛力运动科技有限公司 | Preparation and application method of Anji white tea quercetin microcapsules |
| CN113082080A (en) * | 2021-02-23 | 2021-07-09 | 浙江大学 | Application of illicium plants or extracts thereof in preparation of anti-animal virus drugs |
| CN113150049A (en) * | 2020-04-20 | 2021-07-23 | 中南大学 | Cyclocarya paliurus extract and application thereof in resisting gout and reducing uric acid |
| CN115501268A (en) * | 2022-10-11 | 2022-12-23 | 郑英 | Gel containing fructus anethi extract and preparation method thereof |
| CN117720505A (en) * | 2024-02-07 | 2024-03-19 | 山东省食品药品检验研究院 | New compound separated from star anise root and separation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634412A (en) * | 2004-10-29 | 2005-07-06 | 张平 | Lanceleaf anisetree freeze dried and preparation method thereof |
-
2011
- 2011-09-21 CN CN201110281693A patent/CN102302555A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634412A (en) * | 2004-10-29 | 2005-07-06 | 张平 | Lanceleaf anisetree freeze dried and preparation method thereof |
Non-Patent Citations (7)
| Title |
|---|
| 俞建平等,1: "假地枫皮中黄酮类化合物研究", 《中国现代应用药学》 * |
| 俞建平等,1: "高效液相色谱法测定假地枫皮中槲皮苷的含量", 《医药导报》 * |
| 夏道宗等,1: "黄酮类化合物防治高尿酸血症和痛风的研究进展", 《中国药学杂志》 * |
| 姚芳芳等: "槲皮素对高尿酸血症大鼠黄嘌呤氧化酶和腺苷脱氨酶活性的影响", 《郑州大学学报（医学版）》 * |
| 王苏,1: "红茴香注射液总黄酮的含量测定", 《中国现代应用药学》 * |
| 谢德隆等,1: "红茴香注射液中总黄酮含量的测定", 《中成药》 * |
| 郑成: "红茴香及红茴香注射液指纹图谱的研究", 《中国优秀硕士学位论文全文数据库，医药卫生科技辑》 * |
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| CN102688294A (en) * | 2012-06-13 | 2012-09-26 | 浙江农林大学 | Illicium henryi-contained anti-virus ointment for external use and preparation method and application thereof |
| CN102935133A (en) * | 2012-12-08 | 2013-02-20 | 南京正宽医药科技有限公司 | Traditional Chinese medicine composition for treating rheumatic arthritis as well as preparation method and application thereof |
| CN104586937A (en) * | 2013-11-01 | 2015-05-06 | 浙江泰康药业集团有限公司 | Preparation method of illicium plants injection |
| CN106974921B (en) * | 2016-01-15 | 2020-11-03 | 滨州医学院 | Preparation of 2R-cardiospermin-5-p-hydroxybenzoate and application thereof in preparation of drugs for treating rheumatoid arthritis |
| CN106974921A (en) * | 2016-01-15 | 2017-07-25 | 滨州医学院 | 2R-cardiospermin-5-p-hydroxybenzoate preparation and its application in drugs for rheumatoid arthritis is prepared |
| CN105816524B (en) * | 2016-04-18 | 2020-04-03 | 广西壮族自治区中国科学院广西植物研究所 | Cortex illicii anti-inflammatory active extract and preparation method and application thereof |
| CN105816524A (en) * | 2016-04-18 | 2016-08-03 | 广西壮族自治区中国科学院广西植物研究所 | Anti-inflammatory active cortex illicii extract as well as preparation method and application thereof |
| CN106728137A (en) * | 2016-11-24 | 2017-05-31 | 广州和匠科技有限公司 | It is a kind of that the preparation method for preventing and treating hyperuricemia and gout medicine-food two-purpose monomer is extracted from coffee |
| CN106389597A (en) * | 2016-11-24 | 2017-02-15 | 广州和匠科技有限公司 | Method for efficiently extracting edible and medicinal monomers preventing and treating hyperuricemia and gout from coffee |
| CN108295052A (en) * | 2017-12-28 | 2018-07-20 | 中国人民解放军第二军医大学 | The application of I. henry Diels glycosides ether and composition in urarthritis |
| CN108392509A (en) * | 2018-05-18 | 2018-08-14 | 华中农业大学 | A kind of Pollen Brassicae campestris extract and application thereof |
| CN108392509B (en) * | 2018-05-18 | 2021-07-02 | 华中农业大学 | Rape pollen extract and application thereof |
| CN115154476A (en) * | 2020-04-20 | 2022-10-11 | 中南大学 | Cyclocarya paliurus extract and application thereof in resisting gout and reducing uric acid |
| CN115154476B (en) * | 2020-04-20 | 2023-10-27 | 中南大学 | Cyclocarya paliurus extract and application thereof in resisting gout and reducing uric acid |
| CN113150049A (en) * | 2020-04-20 | 2021-07-23 | 中南大学 | Cyclocarya paliurus extract and application thereof in resisting gout and reducing uric acid |
| CN112089692A (en) * | 2020-09-10 | 2020-12-18 | 浙江赛沛力运动科技有限公司 | Preparation and application method of Anji white tea quercetin microcapsules |
| CN113082080B (en) * | 2021-02-23 | 2022-11-15 | 浙江大学 | Application of illicium plants or extracts thereof in preparation of anti-animal virus drugs |
| CN113082080A (en) * | 2021-02-23 | 2021-07-09 | 浙江大学 | Application of illicium plants or extracts thereof in preparation of anti-animal virus drugs |
| CN115501268A (en) * | 2022-10-11 | 2022-12-23 | 郑英 | Gel containing fructus anethi extract and preparation method thereof |
| CN115501268B (en) * | 2022-10-11 | 2024-01-12 | 郑英 | Gel containing fennel extract and preparation method thereof |
| CN117720505A (en) * | 2024-02-07 | 2024-03-19 | 山东省食品药品检验研究院 | New compound separated from star anise root and separation method and application thereof |
| CN117720505B (en) * | 2024-02-07 | 2024-04-16 | 山东省食品药品检验研究院 | New compound separated from star anise root and separation method and application thereof |
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