CN104585011A - Method for promoting porphyra haitanensis conchospore adhesion and germination - Google Patents
Method for promoting porphyra haitanensis conchospore adhesion and germination Download PDFInfo
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- CN104585011A CN104585011A CN201410698343.XA CN201410698343A CN104585011A CN 104585011 A CN104585011 A CN 104585011A CN 201410698343 A CN201410698343 A CN 201410698343A CN 104585011 A CN104585011 A CN 104585011A
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Abstract
A method for promoting porphyra haitanensis conchospore adhesion and germination comprises the step 1 that agaro-oligosaccharides is prepared with seawater to be 50-250 micrograms per milliliter in concentration and is mixed to be uniform, so an agaro-oligosaccharide and seawater solution is obtained; the step 2 that a porphyra haitanensis web curtain where conchospore seedlings are adhered is soaked in the prepared agaro-oligosaccharide and seawater solution and stays standing for 0.5 h-2 h, wherein illumination intensity is 33,000-80,000 Lx; the step 3 that the density of the seedlings on a web is detected, and the seedlings are moved from a warehouse to be placed into the sea. The method is simple in technology and operation, capable of lowering labor intensity and simplifying working procedures, low in treatment cost and meanwhile capable of effectively improving the germination capacity of adhered conchospores, improving the growth state of porphyra haitanensis seedlings and enhancing the resistance to pathogenic microorganisms and bad environments. Besides, the quality of porphyra haitanensis conchospores is good, and production benefits are greatly improved.
Description
Technical field
The present invention relates to a kind of method promoting Conchospores of Porphyra Hait Anensis stick, especially relate to a kind of in indoor promotion Porphyra haitanensis shell conchocelis the conchospore attachment of diffusing and the method sprouted.
Background technology
Porphyra haitanensis is natural healthy food, is the distinctive traditional cultivation economical alga of China.Have the history that nearly 40 years full artificial cultivations are produced, nearly about 300,000 mu of the area of cultivation at present, output more than 200,000 tons, nearly 2,000,000,000 yuan of the gross output value.The feature of Porphyra haitanensis industry is less investment, relates to personnel many, and manual ratio is high, has good social benefit and economic benefit.
The whole process of the full artificial cultivation of Porphyra haitanensis comprises carpospore and free conchocelis inoculation shell, and------accelerating diffuses conchospore, and---conchospore attachment lace curtaining---seedling sprouting---such as to form at the link to cultivate shell conchocelis.The technology of cultivating shell conchocelis has had comparatively quantum jump, if publication number is the patent of invention " a kind of indoor raising of seedling method of Porphyra haitanensis seedling " of CN102415329A, discloses shell conchocelis string seedling management in nursery pond, improves the method for cleaning; Publication number is the patent of invention " a kind of ecological control method of Porphyra haitanensis nursery " of CN1561690, discloses the unicellular algas such as diatom that to be ingested by copepoda, and the assorted algae of cleaning in time, ensures that shell conchocelis has better growing conditions.
Porphyra haitanensis itself has and does not have single spore, and therefore, conchospore number and quality directly can have influence on quantity and the quality of the seedling needed for breeding production, and conchospore attachment, sprouting ability are by the growth of seedling after directly having influence on.Conchospore individuality is little, is difficult to detect in time, once find the seedling numbers in mariculture area and mass deficiency, fills the gaps with seedlings and affect last income by having little time.Visible Conchospores of Porphyra Hait Anensis attachment is very crucial in full artificial cultivation with sprouting.
The conchospore that diffuses under Porphyra haitanensis nature is generally complete between spring tide flood season.People synchronously diffuse to impel conchospore, are often placed on by shell conchocelis marine greatly, by the stimulation of trend, realize synchronously diffusing.After conchospore diffuses, spill conchospore sea water solution in sea area, conchospore is attached to and hangs on the lace curtaining in sea area in advance, therefore lace curtaining adheres to the impact of conchospore by key elements such as the environment in sea area, conchospore quantity and quality.Also have part raiser to take the method for dying cloth, namely gather conchospore, be immersed in by lace curtaining in conchospore seawater, can have observed and lace curtaining adheres to conchospore, method of dying cloth has higher stability aborning than bold and vigorous spore fluid.The conchospore diffused is attached on lace curtaining, and belong to the link between nursery and cultivation, concerned degree is not high.
Oligosaccharides is applied in aquatic products seedling field to some extent as immunopotentiator, as the patent of invention " application of agaropectin oligose in Porphyra haitanensis immunopotentiator and application method thereof " of publication number to be the patent of invention " a kind of alginic acid oligosaccharides control sea-tangle take off seedling and rotten seedling-growing method " of CN1736162A and publication number be CN102599173A, all carry out in the cultivation stage, but the labour intensity of processing procedure is large, and processing cost is higher.Therefore how to improve the quality in seedling stage, strengthen the resistance to pathogenic microorganism and adverse circumstances, provide the conchospore seedling of health most important.
Summary of the invention
Technical problem to be solved by this invention is to provide that a kind of technique is simple to operation, processing cost is low and the method for matter measured promotion Conchospores of Porphyra Hait Anensis stick.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of method promoting Conchospores of Porphyra Hait Anensis stick, is characterized in that comprising the following steps:
1) by the concentration of agaropectin oligose with seawater preparation 50 ~ 250 μ g/ml, stir, obtain agaropectin oligose sea water solution;
2) the Porphyra haitanensis lace curtaining with conchospore seedling is immersed in the agaropectin oligose sea water solution of above-mentioned preparation, leaves standstill 0.5h ~ 2h; Intensity of illumination is 33000-8000Lx;
3) detect the online density of seedling, outbound is plunged into the commercial sea.
As preferably, described step 1) agaropectin oligose be the catabolite coming from red seaweed polysaccharide-agar-agar, mean molecule quantity is at 324-1620Da.
As improvement, described step 2) the specific operation process of Porphyra haitanensis lace curtaining with conchospore seedling be: after shell conchocelis diffuses conchospore, lace curtaining to be collected seedling is immersed in the seawater of conchospore, according to the quantity of 1 mu of seedling net 1.2-1.8, hundred million conchospores, can observe and lace curtaining adheres to conchospore, adopt flowing water or stirring slowly.
As improvement, on described lace curtaining, every centimetre is actually attached with 300-600 conchospore, and solid density is at every centimetre of 600-900 conchospore.
Compared with prior art, the invention has the advantages that: present invention process is simple to operation, effectively can reduce labour intensity, alleviate working procedure, and processing cost is lower, also effectively can improves attachment conchospore germination ability simultaneously, improve the growth conditions of porphyra haitanensis seedling, strengthen the resistance of porphyra haitanensis seedling to pathogenic microorganism and adverse circumstances, the quality of Porphyra haitanensis spore is good, considerably increases quality and the productivity effect in seedling stage.
Accompanying drawing explanation
Fig. 1 a-1b with after 100 μ g/ml agaropectin oligose process conchospores, at the contrast picture of a water Porphyra haitanensis of sea area cultivation;
Fig. 2 with after 100 μ g/ml agaropectin oligose process conchospores, at the contrast picture of a water Porphyra haitanensis of sea area cultivation.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
Agaropectin oligose seawater is made into the solution that concentration is 100 μ g/ml, by the lace curtaining (lace curtaining density centimetre 400 conchospores) with conchospore, is immersed in agaropectin oligose sea water solution, leave standstill 1.5h.Intensity of illumination is 15000Lx.After lace curtaining is handled well, hang on the raft frame of sea area.
Table 1 is the correction data of 100ug/ml agaropectin oligose solution-treated with the lace curtaining 2h of conchospore.
The process of table 1 agaropectin oligose is on the impact of sea area one water Porphyra haitanensis situation
Embodiment 2
Agaropectin oligose seawater is made into the solution that concentration is 50 μ g/ml, by the lace curtaining (lace curtaining density centimetre 300 conchospores) with conchospore, is immersed in agaropectin oligose sea water solution, leave standstill 1h.Intensity of illumination is 30000Lx.After lace curtaining is handled well, hang on the raft frame of sea area.
Table 2 is 50ug/ml agaropectin oligose solution-treated correction datas with the lace curtaining 2h of conchospore.
The process of table 2 agaropectin oligose is on the impact of sea area one water Porphyra haitanensis situation
Embodiment 3
Agaropectin oligose seawater is made into the solution that concentration is 250 μ g/ml, by the lace curtaining (lace curtaining density centimetre 600 conchospores) with conchospore, is immersed in agaropectin oligose sea water solution, leave standstill 2h.Intensity of illumination is 10000Lx.After lace curtaining is handled well, hang on the raft frame of sea area.
Table 3 is 250ug/ml agaropectin oligose solution-treated correction datas with the lace curtaining 2h of conchospore.
The process of table 3 agaropectin oligose is on the impact of sea area one water Porphyra haitanensis situation
Embodiment 4
Agaropectin oligose seawater is made into the solution that concentration is 150 μ g/ml, by the lace curtaining (lace curtaining density centimetre 500 conchospores) with conchospore, is immersed in agaropectin oligose sea water solution, leave standstill 0.5h.Intensity of illumination is 33000Lx.After lace curtaining is handled well, hang on the raft frame of sea area.
Table 4 is 150ug/ml agaropectin oligose solution-treated correction datas with the lace curtaining 2h of conchospore.
The process of table 4 agaropectin oligose is on the impact of sea area one water Porphyra haitanensis situation
Claims (4)
1. promote a method for Conchospores of Porphyra Hait Anensis stick, it is characterized in that comprising the following steps:
1) by the concentration of agaropectin oligose with seawater preparation 50 ~ 250 μ g/ml, stir, obtain agaropectin oligose sea water solution;
2) the Porphyra haitanensis lace curtaining with conchospore seedling is immersed in the agaropectin oligose sea water solution of above-mentioned preparation, leaves standstill 0.5h ~ 2h; Intensity of illumination is 33000 ~ 8000Lx;
3) detect the online density of seedling, outbound is plunged into the commercial sea.
2. method according to claim 1, is characterized in that: described step 1) agaropectin oligose be the catabolite coming from red seaweed polysaccharide-agar-agar, mean molecule quantity is at 324-1620Da.
3. method according to claim 1, it is characterized in that: described step 2) the specific operation process of Porphyra haitanensis lace curtaining with conchospore seedling be: after shell conchocelis diffuses conchospore, lace curtaining to be collected seedling is immersed in the seawater of conchospore, according to the quantity of 1 mu of seedling net, 1.2 ~ 1.8 hundred million conchospores, can observe and lace curtaining adheres to conchospore, adopt flowing water or stirring slowly.
4. method according to claim 3, is characterized in that: on described lace curtaining, every centimetre is actually attached with 300-600 conchospore.
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| CN201410698343.XA CN104585011B (en) | 2014-11-27 | 2014-11-27 | Method for promoting porphyra haitanensis conchospore adhesion and germination |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489714A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | Porphyra haitanensis seedling rearing room direct nursery technique |
| CN108300676A (en) * | 2018-02-10 | 2018-07-20 | 厦门昶青生物科技有限公司 | A kind of red seaweed polysaccharide composite bacteria agent and its preparation method and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008148621A (en) * | 2006-12-18 | 2008-07-03 | Hamashoku:Kk | Method for raising nemacystus decipiens on land, and method for culturing spore of nemacystus decipiens |
| CN102599173A (en) * | 2012-01-11 | 2012-07-25 | 宁波大学 | Application of agaro-oligosaccharide in porphyra haitanensis immunopotentiator and application method thereof |
| CN103651095A (en) * | 2013-12-26 | 2014-03-26 | 安徽理工大学 | Large-scale seaweed seedling culturing and transplanting method |
-
2014
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008148621A (en) * | 2006-12-18 | 2008-07-03 | Hamashoku:Kk | Method for raising nemacystus decipiens on land, and method for culturing spore of nemacystus decipiens |
| CN102599173A (en) * | 2012-01-11 | 2012-07-25 | 宁波大学 | Application of agaro-oligosaccharide in porphyra haitanensis immunopotentiator and application method thereof |
| CN103651095A (en) * | 2013-12-26 | 2014-03-26 | 安徽理工大学 | Large-scale seaweed seedling culturing and transplanting method |
Non-Patent Citations (2)
| Title |
|---|
| S.C. AGRAWAL: "Factors Affecting Spore Germination in Algae — review", 《FOLIA MICROBIOL.》 * |
| 刘方方 等: "琼胶寡糖对坛紫菜抗烂性的诱导效应", 《宁波大学学报（理工版）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489714A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | Porphyra haitanensis seedling rearing room direct nursery technique |
| CN108300676A (en) * | 2018-02-10 | 2018-07-20 | 厦门昶青生物科技有限公司 | A kind of red seaweed polysaccharide composite bacteria agent and its preparation method and application |
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| CN104585011B (en) | 2017-01-18 |
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