CN104568771A - Economical, portable and efficient grading method for maturity of fresh tobacco leaves of flue-cured tobaccos - Google Patents
Economical, portable and efficient grading method for maturity of fresh tobacco leaves of flue-cured tobaccos Download PDFInfo
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Abstract
The invention discloses an economical, portable and efficient grading method for maturity of fresh tobacco leaves of flue-cured tobaccos. The grading method comprises the following steps: evaluating to-be-tested tobacco leaves, wherein the evaluation content comprises visual inspection on botanical characters of the tobacco leaves, and detection on the content of chlorophyll a and carotenoid of the tobacco leaves, if the evaluation result reaches the corresponding index, judging into corresponding maturity grade; and picking up the tobacco leaves of which the maturity grade accords with the requirements according to the individual requirements. According to the method, by combination of the content detection on the chlorophyll a and carotenoid in the tobacco leaves and the visual inspection of the appearance characteristics of the tobacco leaves, a characteristic grading index is provided for the flue-cured tobacco varieties with faint scent tobacco leaf in three southwest tobacco-growing areas of K326, honghua dajinyuan (Chinese character) and Yunnan tobacco 87; grading of the maturity of fresh tobacco leaves is carried out by adopting the method; supply of reasonable choice for scientifically harvesting the ripen tobacco leaves with different varieties and full play of genetic potential of variety and quality are facilitated; the aromatic style of the fainted scent tobacco leaves is strengthened; the smoking quality of the tobacco leaves is improved; and the grading method is especially suitable for popularization and application in the fainted scent tobacco leaf planting areas in the southwest tobacco-growing areas.
Description
Technical field
The invention belongs to Maturity of Tobacco Leaf classification technique field, be specifically related to the stage division of a kind of economy, convenient, efficient fresh tobacco leaf of flue-cured tobacco degree of ripeness.
Background technology
Leaf tobacco production is the basis of cigarette industry, and the quality of quality of tobacco plays a part very important to cigarette quality.Maturity of Tobacco Leaf is the first factor determining flue cured tobacco quality quality.Fresh tobacco leaf degree of ripeness is divided into physiological makeup and technical maturity.When blade grows up to last size, when dry-matter accumulation reaches maximal value in blade, just reach physiological maturation, therefore claim physiological maturity.Reach through science modulation after the tobacco leaf picking of physiological maturity, output is the highest, but from quality point, is far from reaching the optimum condition of cigarette process requirement.Field fresh tobacco leaf only after physiological maturity again through growing after a while, just can reach the requirement substantially meeting cigarette process processing, tobacco leaf is at this moment called technical maturity.
During current sound tobacco is produced, divide fresh tobacco leaf degree of ripeness and be usually changed to master with Flue-cured, main offshoot and Ye Se, with reference to the change of blade face, leaf margin, blade tip, auricle, the Maturity of Tobacco Leaf at three positions, upper, middle and lower is divided into M1 respectively: raw blue or green, M2: undercure, M3: still ripe, M4: ripe, M5: overdone, 5 ranks, and specify that higher grade, degree of ripeness is higher.The optimal harvest time of tobacco leaf is inferior leads 2-3 level respectively, middle leaf 3-4 level, upper leaf 4 grades.But the subjective judgement of this determination methods Main Basis naked eyes, operationally because different people's criterions often produces larger difference.And different according to industrial requirement, ecologic environment is different and breed difference, required degree of ripeness grade is differentiated often.
Except range estimation, classification is carried out to Maturity of Tobacco Leaf and also has spectrographic method, colorimetric block-regulations etc., but these methods or need the chromatogram collection instrument of purchasing expensive, or the impact of examined environment is larger, or data analysis is too loaded down with trivial details, on using, be all subject to the restriction of certain condition, be difficult to realize field fresh tobacco leaf degree of ripeness fast, the stable and judgement of economy.Based on this, the present invention aims to provide the stage division of a kind of economy, convenient, efficient fresh tobacco leaf of flue-cured tobacco degree of ripeness, carries out accurate quantification to maturity classification, improves the accuracy that Maturity of Tobacco Leaf judges, improves tobacco leaf sucking quality.
Summary of the invention
The object of the present invention is to provide the stage division of a kind of economy, convenient, efficient fresh tobacco leaf of flue-cured tobacco degree of ripeness.
The object of the present invention is achieved like this, the stage division of described economy, convenient, efficient fresh tobacco leaf of flue-cured tobacco degree of ripeness is tested and assessed by tobacco leaf to be measured, test and appraisal content comprises the Agronomic trait of range estimation tobacco leaf, the Chlorophyll-a Content detecting tobacco leaf and carotenoid content, then be judged as corresponding maturity grade as evaluating result reaches corresponding index, then pluck the tobacco leaf meeting and pluck the degree of ripeness grade required according to individual demand.
Stage division of the present invention has considered the intrinsic chemical composition of cured tobacco leaf and external Agronomic trait, the content detection of the Chlorophylls and Carotenoids in tobacco leaf is estimated with the appearance characteristics of tobacco leaf and combines, propose distinctive graded index for these three kinds of Southwest tobacco-growing areas representational flue-cured tobacco cultivars of delicate fragrance type tobacco leaf of K326, the large gold dollar of safflower and cloud and mist 87, improve authenticity and the reliability of Maturity of Tobacco Leaf judgement.
Adopt the method for the invention to carry out fresh tobacco leaf maturity classification, contribute to for different cultivars science Mature Tobacco Leaves of gathering provides choose reasonable and give full play to variety and quality genetic potential.Thus be more conducive to realizing cured tobacco leaf perfume quantity foot, flue gas concentration enriches full, and mildness is good, is more conducive to the aromatic style strengthening delicate fragrance type tobacco leaf, promote tobacco leaf sucking quality, be particularly suitable for applying in delicate fragrance type tobacco leaf planting region, Southwestern China cigarette district.
Embodiment
The present invention is further illustrated below, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or replacement, all belong to protection scope of the present invention.
The stage division of economy of the present invention, convenient, efficient fresh tobacco leaf of flue-cured tobacco degree of ripeness is tested and assessed by tobacco leaf to be measured, test and appraisal content comprises the Agronomic trait of range estimation tobacco leaf, the Chlorophyll-a Content detecting tobacco leaf and carotenoid content, then be judged as corresponding maturity grade as evaluating result reaches corresponding index, then pluck the tobacco leaf meeting and pluck the degree of ripeness grade required according to individual demand.
The Agronomic trait of described range estimation tobacco leaf comprises following concrete operation: respectively inferior leads leaf age 60 ~ 70 days, or after pinching 7 ~ 10 days; Middle leaf leaf age 70 ~ 80 days, or after pinching 20 ~ 30 days; Upper leaf leaf age 80 ~ 90 days, or after pinching 40 ~ 50 days sample, during sampling, from cultivation level consistent same vega, Stochastic choice is no less than 3 strain cigarettes, select 2 ~ 3 as tobacco leaf to be measured in the afore mentioned rules time period at the corresponding site of every strain cigarette, the maturity classification standard of lower tobacco leaf is: M1 to M5; The maturity classification standard of middle part tobacco leaf is: M1 to M5; The maturity classification standard of upper tobacco leaf is M1 to M5.
The Chlorophyll-a Content of described detection tobacco leaf and carotenoid content comprise tobacco leaf sampling, detect and analyze and evaluation of result operation, specifically comprise:
A, tobacco leaf sample: respectively inferior leads leaf age 60 ~ 70 days, or after pinching 7 ~ 10 days; Middle leaf leaf age 70 ~ 80 days, or after pinching 20 ~ 30 days; Upper leaf leaf age 80 ~ 90 days, or after pinching 40 ~ 50 days sample, during sampling, from cultivation level consistent same vega, Stochastic choice is no less than 3 strain cigarettes, 2 ~ 3 are selected as tobacco leaf to be measured at the corresponding site of every strain cigarette in the afore mentioned rules time period, through the Agronomic trait of range estimation tobacco leaf, select the tobacco leaf of differing maturity grade in M1 to M5 as the mensuration sample of chlorophyll a and carotenoid.
B, detection are analyzed: tobacco leaf sample to be measured is broken, the ethanolic solution of 96% is added, lixiviate under 30 ~ 40 DEG C of dark conditions, until when blade bleaches completely by the mass volume ratio of 1:20, with 96% ethanolic solution for blank, colorimetric under wavelength 665,649 and 470nm; The content of chlorophyll a calculates according to the following formula: Ca=13.95A665 ﹣ 6.88A649; The content of carotenoid calculates according to the following formula: Cxc=(1000A470 ﹣ 2.05Ca ﹣ 114.8Cb)/245.
C, evaluation of result: for lower tobacco leaf sample: Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M1 at 430 ~ 480 mg/g, carotenoid content; Chlorophyll-a Content at 50 ~ 65 mg/g, is judged as M2 at 320 ~ 380 mg/g, carotenoid content; Chlorophyll-a Content at 40 ~ 45 mg/g, is judged as M3 at 250 ~ 290 mg/g, carotenoid content; Chlorophyll-a Content at 200 ~ 240 mg/g, carotenoid content at 25 ~ 35 mg/g, be judged as M4; Chlorophyll-a Content at 10 ~ 20 mg/g, is judged as M5 at 80 ~ 170 mg/g, carotenoid content;
For middle part tobacco leaf sample: Chlorophyll-a Content at 75 ~ 90 mg/g, is judged as M1 at 430 ~ 470 mg/g, carotenoid content; Chlorophyll-a Content at 65 ~ 70 mg/g, is judged as M2 at 320 ~ 380 mg/g, carotenoid content; Chlorophyll-a Content at 55 ~ 65 mg/g, is judged as M3 at 270 ~ 300 mg/g, carotenoid content; Chlorophyll-a Content at 45 ~ 55 mg/g, is judged as M4 at 230 ~ 255 mg/g, carotenoid content; Chlorophyll-a Content at 40 ~ 45 mg/g, is judged as M5 at 150 ~ 170 mg/g, carotenoid content;
For upper tobacco leaf sample: Chlorophyll-a Content at 90 ~ 100 mg/g, is judged as M1 at 460 ~ 490 mg/g, carotenoid content; Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M2 at 370 ~ 400 mg/g, carotenoid content; Chlorophyll-a Content at 50 ~ 55 mg/g, is judged as M3 at 250 ~ 270 mg/g, carotenoid content; Chlorophyll-a Content at 45 ~ 55 mg/g, is judged as M4 at 220 ~ 250 mg/g, carotenoid content; Chlorophyll-a Content at 25 ~ 40 mg/g, is judged as M5 at 125 ~ 150 mg/g, carotenoid content.
The quantity of described cigarette strain is 3 strains, and tobacco leaf to be measured amounts to 6 ~ 9
Described cigarette strain need be selected from the consistent same vega of cultivation management measure.
The quality of described tobacco leaf sample to be measured is 1g.
Described for lixiviate 96% the volume of ethanolic solution be 20ml.
When described Chlorophyll-a Content and carotenoid content detect, broken tobacco leaf sample to be measured need immerse in ethanolic solution completely, and adds a cover lucifuge lixiviate.
Described under 30 ~ 40 DEG C of dark conditions lixiviate be carry out in insulation can or water bath with thermostatic control.
Described tobacco leaf is any one in K326, the large gold dollar of safflower or cloud and mist 87.
Described classification need select harvest maturity flexibly according to breediness, and the best harvest maturity at each position of cloud and mist 87 kind is respectively: inferior leads M2, middle leaf M2 ~ M3, upper leaf M3 ~ M4; The best harvest maturity at each position of Hongda is respectively: inferior leads M2, middle leaf M3, upper leaf M2 ~ M3; The best harvest maturity at each position of K326 kind is respectively: inferior leads M2 ~ M3, middle leaf M3 ~ M4, upper leaf M3 ~ M4.
embodiment 1
Experiment place: Kunming tobacco company stone forest base, Kunming, Yunnan city
Experimental subjects: the large gold dollar tobacco leaf of safflower
Stage division:
(1) Agronomic trait of tobacco leaf is estimated: respectively inferior leads leaf age 60 ~ 70 days, or after pinching 7 ~ 10 days; Middle leaf leaf age 70 ~ 80 days, or after pinching 20 ~ 30 days; Upper leaf leaf age 80 ~ 90 days, or after pinching 40 ~ 50 days sample, during sampling, from cultivation level consistent same vega, Stochastic choice is no less than 3 strain cigarettes, selects 2 ~ 3 as tobacco leaf to be measured in the afore mentioned rules time period at the corresponding site of every strain cigarette.
(2) detect the Chlorophyll-a Content of tobacco leaf and carotenoid content: the visually result of the Agronomic trait of tobacco leaf, select the tobacco leaf of differing maturity grade in M1 to M5 as the mensuration sample of chlorophyll a and carotenoid.Get 1g tobacco leaf sample to be measured broken, load test tube, add the ethanolic solution 20ml of 96%, broken tobacco leaf sample to be measured is immersed in ethanolic solution completely, add a cover, lixiviate under dark lucifuge condition in 30 ~ 40 DEG C of insulation cans, until when blade bleaches completely, with 96% ethanolic solution for blank, colorimetric under wavelength 665,649 and 470nm; The content of chlorophyll a calculates according to the following formula: Ca=13.95A665 ﹣ 6.88A649; The content of carotenoid calculates according to the following formula: Cxc=(1000A470 ﹣ 2.05Ca ﹣ 114.8Cb)/245.
For lower tobacco leaf sample: Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M1 at 430 ~ 480 mg/g, carotenoid content; Chlorophyll-a Content at 50 ~ 65 mg/g, is judged as M2 at 320 ~ 380 mg/g, carotenoid content; Chlorophyll-a Content at 40 ~ 45 mg/g, is judged as M3 at 250 ~ 290 mg/g, carotenoid content; Chlorophyll-a Content at 200 ~ 240 mg/g, carotenoid content at 25 ~ 35 mg/g, be judged as M4; Chlorophyll-a Content at 10 ~ 20 mg/g, is judged as M5 at 80 ~ 170 mg/g, carotenoid content;
For middle part tobacco leaf sample: Chlorophyll-a Content at 75 ~ 90 mg/g, is judged as M1 at 430 ~ 470 mg/g, carotenoid content; Chlorophyll-a Content at 65 ~ 70 mg/g, is judged as M2 at 320 ~ 380 mg/g, carotenoid content; Chlorophyll-a Content at 55 ~ 65 mg/g, is judged as M3 at 270 ~ 300 mg/g, carotenoid content; Chlorophyll-a Content at 45 ~ 55 mg/g, is judged as M4 at 230 ~ 255 mg/g, carotenoid content; Chlorophyll-a Content at 40 ~ 45 mg/g, is judged as M5 at 150 ~ 170 mg/g, carotenoid content;
For upper tobacco leaf sample: Chlorophyll-a Content at 90 ~ 100 mg/g, is judged as M1 at 460 ~ 490 mg/g, carotenoid content; Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M2 at 370 ~ 400 mg/g, carotenoid content; Chlorophyll-a Content at 50 ~ 55 mg/g, is judged as M3 at 250 ~ 270 mg/g, carotenoid content; Chlorophyll-a Content at 45 ~ 55 mg/g, is judged as M4 at 220 ~ 250 mg/g, carotenoid content; Chlorophyll-a Content at 25 ~ 40 mg/g, is judged as M5 at 125 ~ 150 mg/g, carotenoid content.
Classification results: for lower tobacco leaf sample: Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M1 at 450 ~ 480 mg/g, carotenoid content;
For lower tobacco leaf sample: Chlorophyll-a Content at 50 ~ 65 mg/g, is judged as M2 at 360 ~ 380 mg/g, carotenoid content;
For lower tobacco leaf sample: Chlorophyll-a Content at 40 ~ 45 mg/g, is judged as M3 at 280 ~ 290 mg/g, carotenoid content.
For middle part tobacco leaf sample: Chlorophyll-a Content at 75 ~ 90 mg/g, is judged as M1 at 450 ~ 470 mg/g, carotenoid content;
For middle part tobacco leaf sample: Chlorophyll-a Content at 65 ~ 70 mg/g, is judged as M2 at 350 ~ 380 mg/g, carotenoid content;
For middle part tobacco leaf sample: Chlorophyll-a Content at 55 ~ 65 mg/g, is judged as M3 at 280 ~ 300 mg/g, carotenoid content.
For upper tobacco leaf sample: Chlorophyll-a Content at 90 ~ 100 mg/g, is judged as M1 at 480 ~ 490 mg/g, carotenoid content;
For upper tobacco leaf sample: Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M2 at 390 ~ 400 mg/g, carotenoid content;
For upper tobacco leaf sample: Chlorophyll-a Content at 50 ~ 55 mg/g, is judged as M3 at 260 ~ 270 mg/g, carotenoid content.
embodiment 2
Experiment place: science and technology test base, pasture is sought in Kunming, Yunnan city
Experimental subjects: K326 tobacco leaf
Stage division: with embodiment 1.
Classification results: for lower tobacco leaf sample: Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M1 at 430 ~ 450 mg/g, carotenoid content;
For lower tobacco leaf sample: Chlorophyll-a Content at 50 ~ 65 mg/g, is judged as M2 at 320 ~ 350 mg/g, carotenoid content;
For lower tobacco leaf sample: Chlorophyll-a Content at 40 ~ 45 mg/g, is judged as M3 at 250 ~ 260 mg/g, carotenoid content.
For middle part tobacco leaf sample: Chlorophyll-a Content at 75 ~ 90 mg/g, is judged as M1 at 430 ~ 450 mg/g, carotenoid content;
For middle part tobacco leaf sample: Chlorophyll-a Content at 65 ~ 70 mg/g, is judged as M2 at 320 ~ 350 mg/g, carotenoid content;
For middle part tobacco leaf sample: Chlorophyll-a Content at 55 ~ 65 mg/g, is judged as M3 at 270 ~ 280 mg/g, carotenoid content.
For upper tobacco leaf sample: Chlorophyll-a Content at 90 ~ 100 mg/g, is judged as M1 at 460 ~ 470 mg/g, carotenoid content;
For upper tobacco leaf sample: Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M2 at 370 ~ 380 mg/g, carotenoid content;
For upper tobacco leaf sample: Chlorophyll-a Content at 50 ~ 55 mg/g, is judged as M3 at 250 ~ 260 mg/g, carotenoid content.
embodiment 3
Experiment place: Zhong Ling base, pasture is sought in Kunming, Yunnan city
Experimental subjects: cloud and mist 87 tobacco leaf
Stage division: with embodiment 1.
Classification results: for lower tobacco leaf sample: Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M1 at 450 ~ 460 mg/g, carotenoid content;
For lower tobacco leaf sample: Chlorophyll-a Content at 50 ~ 65 mg/g, is judged as M2 at 350 ~ 360 mg/g, carotenoid content;
For lower tobacco leaf sample: Chlorophyll-a Content at 40 ~ 45 mg/g, is judged as M3 at 260 ~ 280 mg/g, carotenoid content.
For middle part tobacco leaf sample: Chlorophyll-a Content at 75 ~ 90 mg/g, is judged as M1 at 450 ~ 460 mg/g, carotenoid content;
For middle part tobacco leaf sample: Chlorophyll-a Content at 65 ~ 70 mg/g, is judged as M2 at 350 ~ 370 mg/g, carotenoid content;
For middle part tobacco leaf sample: Chlorophyll-a Content at 55 ~ 65 mg/g, is judged as M3 at 280 ~ 290 mg/g, carotenoid content.
For upper tobacco leaf sample: Chlorophyll-a Content at 90 ~ 100 mg/g, is judged as M1 at 470 ~ 480 mg/g, carotenoid content;
For upper tobacco leaf sample: Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M2 at 380 ~ 390 mg/g, carotenoid content;
For upper tobacco leaf sample: Chlorophyll-a Content at 50 ~ 55 mg/g, is judged as M3 at 255 ~ 265 mg/g, carotenoid content.
Claims (10)
1. the stage division of an economy, convenient, efficient fresh tobacco leaf of flue-cured tobacco degree of ripeness, tobacco leaf to be measured is it is characterized in that to test and assess, test and appraisal content comprises the Agronomic trait of range estimation tobacco leaf, the Chlorophyll-a Content detecting tobacco leaf and carotenoid content, then be judged as corresponding maturity grade as evaluating result reaches corresponding index, then pluck the tobacco leaf meeting and pluck the degree of ripeness grade required according to individual demand.
2. stage division according to claim 1, is characterized in that the Agronomic trait of described range estimation tobacco leaf comprises following concrete operation: respectively inferior leads leaf age 60 ~ 70 days, or after pinching 7 ~ 10 days; Middle leaf leaf age 70 ~ 80 days, or after pinching 20 ~ 30 days; Upper leaf leaf age 80 ~ 90 days, or after pinching 40 ~ 50 days sample, during sampling, from cultivation level consistent same vega, Stochastic choice is no less than 3 strain cigarettes, select 2 ~ 3 as tobacco leaf to be measured in the afore mentioned rules time period at the corresponding site of every strain cigarette, the maturity classification standard of lower tobacco leaf is: M1 to M5; The maturity classification standard of middle part tobacco leaf is: M1 to M5; The maturity classification standard of upper tobacco leaf is M1 to M5.
3. stage division according to claim 2, is characterized in that the Chlorophyll-a Content of described detection tobacco leaf and carotenoid content comprise tobacco leaf sampling, detect and analyze and evaluation of result operation, specifically comprises:
A, tobacco leaf sample: respectively inferior leads leaf age 60 ~ 70 days, or after pinching 7 ~ 10 days; Middle leaf leaf age 70 ~ 80 days, or after pinching 20 ~ 30 days; Upper leaf leaf age 80 ~ 90 days, or after pinching 40 ~ 50 days sample, during sampling, from cultivation level consistent same vega, Stochastic choice is no less than 3 strain cigarettes, 2 ~ 3 are selected as tobacco leaf to be measured at the corresponding site of every strain cigarette in the afore mentioned rules time period, through the Agronomic trait of range estimation tobacco leaf, select the tobacco leaf of differing maturity grade in M1 to M5 as the mensuration sample of chlorophyll a and carotenoid;
B, detection are analyzed: tobacco leaf sample to be measured is broken, the ethanolic solution of 96% is added, lixiviate under 30 ~ 40 DEG C of dark conditions, until when blade bleaches completely by the mass volume ratio of 1:20, with 96% ethanolic solution for blank, colorimetric under wavelength 665,649 and 470nm; The content of chlorophyll a calculates according to the following formula: Ca=13.95A665 ﹣ 6.88A649; The content of carotenoid calculates according to the following formula: Cxc=(1000A470 ﹣ 2.05Ca ﹣ 114.8Cb)/245;
C, evaluation of result: for lower tobacco leaf sample: Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M1 at 430 ~ 480 mg/g, carotenoid content; Chlorophyll-a Content at 50 ~ 65 mg/g, is judged as M2 at 320 ~ 380 mg/g, carotenoid content; Chlorophyll-a Content at 40 ~ 45 mg/g, is judged as M3 at 250 ~ 290 mg/g, carotenoid content; Chlorophyll-a Content at 200 ~ 240 mg/g, carotenoid content at 25 ~ 35 mg/g, be judged as M4; Chlorophyll-a Content at 10 ~ 20 mg/g, is judged as M5 at 80 ~ 170 mg/g, carotenoid content;
For middle part tobacco leaf sample: Chlorophyll-a Content at 75 ~ 90 mg/g, is judged as M1 at 430 ~ 470 mg/g, carotenoid content; Chlorophyll-a Content at 65 ~ 70 mg/g, is judged as M2 at 320 ~ 380 mg/g, carotenoid content; Chlorophyll-a Content at 55 ~ 65 mg/g, is judged as M3 at 270 ~ 300 mg/g, carotenoid content; Chlorophyll-a Content at 45 ~ 55 mg/g, is judged as M4 at 230 ~ 255 mg/g, carotenoid content; Chlorophyll-a Content at 40 ~ 45 mg/g, is judged as M5 at 150 ~ 170 mg/g, carotenoid content;
For upper tobacco leaf sample: Chlorophyll-a Content at 90 ~ 100 mg/g, is judged as M1 at 460 ~ 490 mg/g, carotenoid content; Chlorophyll-a Content at 70 ~ 90 mg/g, is judged as M2 at 370 ~ 400 mg/g, carotenoid content; Chlorophyll-a Content at 50 ~ 55 mg/g, is judged as M3 at 250 ~ 270 mg/g, carotenoid content; Chlorophyll-a Content at 45 ~ 55 mg/g, is judged as M4 at 220 ~ 250 mg/g, carotenoid content; Chlorophyll-a Content at 25 ~ 40 mg/g, is judged as M5 at 125 ~ 150 mg/g, carotenoid content.
4. the stage division according to any one of Claims 2 or 3, it is characterized in that the quantity of described cigarette strain is 3 strains, tobacco leaf to be measured amounts to 6 ~ 9.
5. stage division according to claim 3, is characterized in that the quality of described tobacco leaf sample to be measured is 1g.
6. stage division according to claim 3, it is characterized in that described for lixiviate 96% the volume of ethanolic solution be 20ml.
7. stage division according to claim 3, when it is characterized in that described Chlorophyll-a Content and carotenoid content detect, broken tobacco leaf sample to be measured need immerse in ethanolic solution completely, and adds a cover lucifuge lixiviate.
8. stage division according to claim 3, described in it is characterized in that, under 30 ~ 40 DEG C of dark conditions, lixiviate carries out in insulation can or water bath with thermostatic control.
9. the stage division according to any one of claim 1,2,3,5,6,7 or 8, is characterized in that described tobacco leaf is any one in K326, the large gold dollar of safflower or cloud and mist 87.
10. stage division according to claim 9, is characterized in that described classification need select harvest maturity flexibly according to breediness, and the best harvest maturity at each position of cloud and mist 87 kind is respectively: inferior leads M2, middle leaf M2 ~ M3, upper leaf M3 ~ M4; The best harvest maturity at each position of Hongda is respectively: inferior leads M2, middle leaf M3, upper leaf M2 ~ M3; The best harvest maturity at each position of K326 kind is respectively: inferior leads M2 ~ M3, middle leaf M3 ~ M4, upper leaf M3 ~ M4.
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| CN106248591A (en) * | 2016-08-10 | 2016-12-21 | 中国农业科学院烟草研究所 | A kind of Maturity of Tobacco Leaf differentiation utensil and differentiating method thereof |
| CN106442788A (en) * | 2016-09-30 | 2017-02-22 | 中国烟草总公司郑州烟草研究院 | Method for judging quality of fresh tobacco leaf sample in tobacco metabonomics based on pigments |
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| CN114916696A (en) * | 2022-05-18 | 2022-08-19 | 山东潍坊烟草有限公司 | Method for establishing grade and quality judgment standard of fresh tobacco leaves |
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| CN112868524A (en) * | 2021-01-13 | 2021-06-01 | 湖北省烟草科学研究院 | Method for rapidly breeding high-quality burley tobacco variety by using fresh leaves and single leaf weight |
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| CN117589767A (en) * | 2024-01-18 | 2024-02-23 | 北京香田智能科技有限公司 | Tobacco leaf harvesting time determining method, electronic equipment and storage medium |
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