CN104561067A - 一种安全高效的蛋白质转染进入细胞的载体 - Google Patents

一种安全高效的蛋白质转染进入细胞的载体 Download PDF

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Publication number
CN104561067A
CN104561067A CN201410823369.2A CN201410823369A CN104561067A CN 104561067 A CN104561067 A CN 104561067A CN 201410823369 A CN201410823369 A CN 201410823369A CN 104561067 A CN104561067 A CN 104561067A
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CN
China
Prior art keywords
chitosan
carrier
protein
cell
transfection
Prior art date
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Pending
Application number
CN201410823369.2A
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English (en)
Chinese (zh)
Inventor
余细勇
吴岳恒
姜霖
汤顺清
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Guangdong General Hospital
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Guangdong General Hospital
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Publication date
Application filed by Guangdong General Hospital filed Critical Guangdong General Hospital
Priority to CN201410823369.2A priority Critical patent/CN104561067A/zh
Priority to PCT/CN2015/072963 priority patent/WO2016101405A1/fr
Publication of CN104561067A publication Critical patent/CN104561067A/zh
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201410823369.2A 2014-12-24 2014-12-24 一种安全高效的蛋白质转染进入细胞的载体 Pending CN104561067A (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201410823369.2A CN104561067A (zh) 2014-12-24 2014-12-24 一种安全高效的蛋白质转染进入细胞的载体
PCT/CN2015/072963 WO2016101405A1 (fr) 2014-12-24 2015-02-13 Vecteur sûr et efficace pour la transfection de protéine dans une cellule

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410823369.2A CN104561067A (zh) 2014-12-24 2014-12-24 一种安全高效的蛋白质转染进入细胞的载体

Publications (1)

Publication Number Publication Date
CN104561067A true CN104561067A (zh) 2015-04-29

Family

ID=53078145

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410823369.2A Pending CN104561067A (zh) 2014-12-24 2014-12-24 一种安全高效的蛋白质转染进入细胞的载体

Country Status (2)

Country Link
CN (1) CN104561067A (fr)
WO (1) WO2016101405A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106676062A (zh) * 2017-02-20 2017-05-17 余细勇 一种制备心脏祖细胞的方法
CN113583189A (zh) * 2021-08-06 2021-11-02 吉林大学 一种多羟基聚氨酯蛋白质转染试剂、制备方法及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003078576A2 (fr) * 2002-03-12 2003-09-25 Nitto Denko Corporation Vecteurs pour la transfection des cellules eucaryotes
CN1646174A (zh) * 2002-05-14 2005-07-27 日东电工株式会社 可控降解的聚合生物分子或药物载体及其合成方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003078576A2 (fr) * 2002-03-12 2003-09-25 Nitto Denko Corporation Vecteurs pour la transfection des cellules eucaryotes
CN1646174A (zh) * 2002-05-14 2005-07-27 日东电工株式会社 可控降解的聚合生物分子或药物载体及其合成方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
QUAN GAN等: "Chitosan nanoparticle as protein delivery carrier—Systematic examination of fabrication conditions for efficient loading and release", 《COLLOIDS AND SURFACES B: BIOINTERFACES》 *
李英: "水溶性壳聚糖功能化碳纳米管的研究", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106676062A (zh) * 2017-02-20 2017-05-17 余细勇 一种制备心脏祖细胞的方法
CN106676062B (zh) * 2017-02-20 2020-05-01 余细勇 一种制备心脏祖细胞的方法
CN113583189A (zh) * 2021-08-06 2021-11-02 吉林大学 一种多羟基聚氨酯蛋白质转染试剂、制备方法及其应用

Also Published As

Publication number Publication date
WO2016101405A1 (fr) 2016-06-30

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Application publication date: 20150429