CN104560721B - 亚心形扁藻培养基 - Google Patents

亚心形扁藻培养基 Download PDF

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CN104560721B
CN104560721B CN201510054238.7A CN201510054238A CN104560721B CN 104560721 B CN104560721 B CN 104560721B CN 201510054238 A CN201510054238 A CN 201510054238A CN 104560721 B CN104560721 B CN 104560721B
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CN104560721A (zh
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王兆伟
彭小伟
宋佳巍
杜学星
孙小雪
江灵芝
王洁
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Ningbo Fu Tian Bioisystech Co Ltd
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Abstract

本发明提供了一种亚心形扁藻培养基配方，该培养基配方如下：氮盐80‑120mg/L，KH2PO410mg/L，柠檬酸铁5mg/L，Na2EDTA 10‑12mg/L，稀土盐0.5‑1mg/L，生长调节剂0.01‑0.1mg/L，VB10.006mg/L，VB120.05mg/L，ZnSO4·4H2O 23μg/L，MnCl2·4H2O 178μg/L，CuSO4·5H2O 10μg/L，Na2MoO4·2H2O 7.3μg/L，CoCl2·6H2O 12μg/L。本发明所述的培养基在培养扁藻时具有生长速率快、细胞密度大等特点。

Description

亚心形扁藻培养基
技术领域
本发明涉及到藻类培养基，特别的涉及到亚心形扁藻的培养基。
背景技术
目前，海洋微藻种类繁多、生长速度快、适应性强、易于培养。微藻含有某些特异的高附加值生物活性成分，因此被广泛应用于食品工业、水产养殖、医药美容、废水处理和生物能源等领域，有重要经济价值。亚心形扁藻是研究和应用最广泛的微藻之一。
亚心形扁藻为一种海产单细胞浮游藻类，适应性强，上浮性好，生长繁殖迅速，能够合成高浓度的多不饱和脂肪酸PuFA，常作为鱼、虾、贝类幼体的开口饵料及饵料动物的饵料。
目前，亚心形扁藻培养存在的主要问题是藻细胞生长缓慢、倍增时间长。合适的培养基是实现亚心形扁藻高密度培养的重要因素之一。以前使用的扁藻培养基没有加入稀土元素硝酸钬，也没有加入生长调节剂氯吡尿。但申请人的实验表明，加入一定的稀土盐和生长调节剂都可以使得扁藻细胞浓度增高。
总之，使用优化后的培养基培养扁藻具有生长速度快、产量高、成本低的优势。
发明内容
本发明提供了用于亚心形扁藻培养基，该培养基配比如下：
氮盐80-120mg/L，KH2PO4 10mg/L，柠檬酸铁5mg/L，Na2EDTA 10-12mg/L，稀土盐0.5-1mg/L，生长调节剂0.01-0.1mg/L，VB1 0.006mg/L，VB12 0.05mg/L，ZnSO4·4H2O 23μg/L，MnCl2·4H2O 178μg/L，CuSO4·5H2O 10μg/L，Na2MoO4·2H2O 7.3μg/L，CoCl2·6H2O 12μg/L。
优选的，所述稀土盐为硝酸钬，所述生长调节剂为氯吡尿。
优选的，所述氮盐为硝酸钾、硝酸铵、氯化铵或者尿素中的一种。
优选的，所述氮盐为硝酸钾，且硝酸钾取100mg/L，Na2EDTA 10mg/L，硝酸钬0.5mg/L，氯吡尿0.01mg/L。
优选的，所述氮盐为尿素，且尿素取尿素80mg/L，Na2EDTA 12mg/L，硝酸钬1mg/L，氯吡尿0.1mg/L。
本发明所述的培养基在培养扁藻时具有生长速度快、产量高、成本低的优势。下面的实施例以及对比实验可以清楚地反应本发明所述培养的特点。
附图说明
图1为本发明实施例1和实施例2中OD700含量随时间变化图。
具体实施方式
实施例1
(1)配置如下配比的海水培养基：
硝酸钾取100mg/L，KH2PO4 10mg/L，柠檬酸铁5mg/L，Na2EDTA 10mg/L，硝酸钬0.5mg/L，氯吡尿0.01mg/L，VB1 0.006mg/L，VB12 0.05mg/L，ZnSO4·4H2O 23μg/L，MnCl2·4H2O 178μg/L，CuSO4·5H2O 10μg/L，Na2MoO4·2H2O 7.3μg/L，CoCl2·6H2O 12μg/L。
(2)取对数生长期的亚心形扁藻接种到该培养基中进行培养，每天光照10小时，光照强度约为50μmol/(m2·s)培箱温度控制在25摄氏度左右，每隔一段时间记录培养基中扁藻的OD700和细胞密度。
实施例2
(1)配置如下配比的海水培养基：
尿素80mg/L，KH2PO4 10mg/L，柠檬酸铁5mg/L，Na2EDTA 12mg/L，硝酸钬1mg/L，氯吡尿0.1mg/L，VB1 0.006mg/L，VB12 0.05mg/L，ZnSO4·4H2O 23μg/L，MnCl2·4H2O 178μg/L，CuSO4·5H2O 10μg/L，Na2MoO4·2H2O 7.3μg/L，CoCl2·6H2O 12μg/L。
(2)取对数生长期的扁藻接种到该培养基中进行培养，每天光照10小时，光照强度约为50μmol/(m2·s)培箱温度控制在25摄氏度左右，每2天记录培养基中扁藻的OD700细胞密度。
培养基对比试验
使用海水f/2培养基作为对照培养基，与实例1-2进行对比，对比的结果如下表所示(*104个/mL)：
从该表可以看出从该表可以看出，使用本发明所提供的培养基比使用对比培养基更快速地使亚心形扁藻增殖，而且藻能达到的最高浓度也高于对照培养基约为27％。说明了本发明供的培养基具有明显优势。
本领域技术人员可以根据本发明公开的内容和所掌握的本领域技术对本发明内容作出替换或变型，但是这些替换或变型都不应视为脱离本发明构思的，这些替换或变型均在本发明要求保护的权利范围内。

Claims (2)

1.一种亚心形扁藻培养基，其特征在于，该培养基配比如下：
硝酸钾100mg/L，KH2PO4 10mg/L，柠檬酸铁5mg/L，Na2EDTA 10mg/L，硝酸钬0.5mg/L，氯吡尿0.01mg/L，VB1 0.006mg/L，VB12 0.05mg/L，ZnSO4·4H2O 23μg/L，MnCl2·4H2O 178μg/L，CuSO4·5H2O 10μg/L，Na2MoO4·2H2O 7.3μg/L，CoCl2·6H2O 12μg/L。
2.一种亚心形扁藻培养基，其特征在于，该培养基配比如下：
尿素80mg/L，KH2PO4 10mg/L，柠檬酸铁5mg/L，Na2EDTA 12mg/L，硝酸钬1mg/L，氯吡尿0.1mg/L，VB1 0.006mg/L，VB12 0.05mg/L，ZnSO4·4H2O 23μg/L，MnCl2·4H2O 178μg/L，CuSO4·5H2O 10μg/L，Na2MoO4·2H2O 7.3μg/L，CoCl2·6H2O 12μg/L。
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CN104813915B (zh) * 2015-05-21 2017-02-22 北海市源龙珍珠有限公司 一种扁藻的采集及培育方法
CN104946538B (zh) * 2015-07-13 2018-04-20 宁波浮田生物技术有限公司 一种三角褐指藻培养基
CN107699538B (zh) * 2017-12-05 2020-11-03 杭州富阳优信科技有限公司 一种培养亚心型扁藻的方法
CN112094749B (zh) * 2020-09-30 2022-12-16 广东海洋大学 一种用于亚心形扁藻中继培养的高比重海水培养方法

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CN103960118A (zh) * 2014-05-26 2014-08-06 临沂大学 一种利用淀粉厂废水养殖青岛大扁藻的培养基及培养方法
CN104046651A (zh) * 2013-03-15 2014-09-17 中国科学院宁波材料技术与工程研究所 一种提高微藻产氢效率的方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104046651A (zh) * 2013-03-15 2014-09-17 中国科学院宁波材料技术与工程研究所 一种提高微藻产氢效率的方法
CN103960118A (zh) * 2014-05-26 2014-08-06 临沂大学 一种利用淀粉厂废水养殖青岛大扁藻的培养基及培养方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
不同培养基培养的亚心形扁藻直接光照产氢;郭祯 等;《武汉理工大学学报》;20061130;第28卷;233-239 *

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