CN104535570A - Device for testing single molecule force spectroscopy based on centrifugal force - Google Patents
Device for testing single molecule force spectroscopy based on centrifugal force Download PDFInfo
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- CN104535570A CN104535570A CN201410819884.3A CN201410819884A CN104535570A CN 104535570 A CN104535570 A CN 104535570A CN 201410819884 A CN201410819884 A CN 201410819884A CN 104535570 A CN104535570 A CN 104535570A
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- force
- centrifugal force
- single molecule
- mechanical arm
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Abstract
The invention discloses a device for testing single molecule force spectroscopy based on centrifugal force. The device comprises a microscope and a microsphere connected with one end of a molecular sample, wherein the other end of the molecular sample is connected with the front side of a molecular substrate; the molecular substrate is vertically fixed above a rotary mechanical arm; the microscope, a camera, a photoelectric rotary connector, an electro-optic converter and a counter weight are sequentially arranged on the rotary mechanical arm on the front side of the molecular substrate; a light source is arranged on the rotary mechanical arm on the back surface of the molecular substrate; and a rotating platform is arranged below the rotary mechanical arm. The device disclosed by the invention is simple in structure and convenient to operate, multiple single molecules can be concurrently tested, the range capable of applying the acting force is very wide, and the device is suitable for concurrent high-flux measurement of multiple different single molecules.
Description
Technical field
The present invention relates to single molecule force spectroscopy fields of measurement, especially a kind of device of the high flux measurement that single molecule force spectroscopy walked abreast based on centrifugal force is related to, specifically, be a kind ofly utilize centrifugal force to produce acting force to multiple microballoon simultaneously thus observe molecule that microballoon connects length variations or and the device that changes of other species operating distance under this acting force.
Background technology
Present single molecule force spectroscopy device mainly comprises: light tweezer, magnetic tweezer and atomic force microscope etc.Light tweezer forms gradient force ligh trap by focused beam, thus produce acting force to the microballoon in ligh trap; Magnetic tweezer by magnets produce magnetic fields gradient, thus produces acting force to magnetic bead; And then the molecule be connected on microballoon (magnetic bead) is stretched.Atomic force microscope utilizes probe tip adhesion molecule to carry out the test of tensile force spectrum to it.But light tweezer, atomic force microscope and general magnetic tweezer can only be measured individual molecule, can not obtain the experimental result with statistical significance.In recent years, holographic optical forceps device and special magnetic tweezer can be measured two or several molecule simultaneously simultaneously, but its application is very limited.Therefore, a kind of device that can carry out parallel measurement to high flux unimolecule is necessary.
Summary of the invention
The object of the invention is to overcome deficiency of the prior art, a kind of brand-new single molecule force spectroscopy proving installation is provided, device is simple, easy to operate, and concurrent testing can be carried out to multiple unimolecule, the scope that simultaneously can apply acting force is very wide in range, is applicable to the multiple different unimolecule high flux that walks abreast and measures.
The object of the invention is to be achieved through the following technical solutions:
A kind of based on centrifugal force test single molecule force spectroscopy device, the microballoon comprising microscope and be connected with molecular sample one end, the other end of described molecular sample is connected with the front of molecule substrate, described molecule substrate transverse is fixed on above rotating mechanical arm, the rotating mechanical arm of described molecule substrate face is disposed with microscope, camera, photoelectricity rotary connector, electrical to optical converter and counterweight, the rotating mechanical arm of molecule substrate back is provided with light source, below described rotating mechanical arm, is provided with rotation platform.
Described microscopical object lens face microballoon and light source.
Described microscope is optical microscope.
The diameter of described microballoon is 1 micron to 25 microns.
Described microballoon is made up of silicon ball, polystyrene microsphere or magnetic bead wherein one or more.
Described light source is LED.
Described camera is Ethernet camera.
Described molecule substrate is slide.
Compared with prior art, the beneficial effect that technical scheme of the present invention is brought is:
1, the molecule substrate in the present invention can be arranged a lot of the microballoons be connected with molecular sample simultaneously, and apply centrifugal force to microballoon by rotation platform simultaneously, the centrifugal force produced microballoon is identical, has very high homogeneity.
2, in the present invention, rotation platform can from skin ox level to receiving ox level to the acting force scope that molecular sample applies.
3, the device energy real-time follow-up microballoon position in the present invention record, therefore can concurrent testing single molecule force spectroscopy fast and accurately.
4, apparatus of the present invention one-piece construction is simple, easy and simple to handle during use.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Reference numeral: 1-light source 2-molecule substrate 3-microballoon 4-microscope 5-camera 6-photoelectricity rotary connector 7-electrical to optical converter 8-counterweight 9-rotating mechanical arm 10-rotation platform
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described:
As shown in Figure 1, a kind of based on centrifugal force test single molecule force spectroscopy device, the microballoon 3 comprising microscope 4 and be connected with molecular sample one end, microscope 4 is optical microscopes, in order to amplify microballoon 3 and to be imaged on camera 5, microballoon 3 is that the silicon ball of diameter 1 micron to 25 microns, polystyrene microsphere or magnetic bead wherein one or more are formed; The other end of molecular sample is connected with the front of molecule substrate 2, molecule substrate 2 is vertically fixed on the top of rotating mechanical arm 9, the below of rotating mechanical arm 9 is fixed on rotation platform 10, rotation platform 10 is High Rotation Speed platform, rotation platform 10 is provided with controller, in order to it rotates by software control, provide centrifugal force.
The rotating mechanical arm 9 being positioned at molecule substrate 2 front has been fixedly connected sequentially microscope 4, camera 5, photoelectricity rotary connector 6, electrical to optical converter 7 and counterweight 8, and camera 5 is Ethernet camera, in order to the imaging of observed and recorded molecular sample; Photoelectricity rotary connector 6, in order to solve the transmission of light signal and electric signal between rotary part, ensures that light signal and electric signal do not interrupt because of rotation; The electric signal that camera 5 obtains is converted to light signal and is transmitted by optical fiber by electrical to optical converter 7, the light signal in optical fiber is converted to electric signal simultaneously and is transferred on computing machine by Ethernet, and counterweight 8 is for regulating system mobile equilibrium.
The rotating mechanical arm 9 at molecule substrate 2 back side is fixedly installed light source 1, and light source 1 is LED, and launch high energy red-light LED light, the object lens of microscope 4 face microballoon 3 and light source 1, and the light of light source 1 is radiated on microballoon 3, facilitates observed and recorded.
Concrete using method of the present invention is as follows: control High Rotation Speed platform 10 by control software design and rotate with certain rotating speed, for system provides centrifugal force; Microballoon 3 is observed after applying centrifugal force in the motion of molecule substrate 2 surface three dimension and with certain hour interval record image by Ethernet camera 5, thus can determine that microballoon 3 is at the surface location of molecule substrate 2 at regular intervals, can infer when after generation centrifugal force after High Rotation Speed platform 10 rotates thus, namely after molecule is subject to constant force, its time dependent deformation process or the intermolecular the interaction distance change procedure of inhomogeneity.By controlling the rotating speed of High Rotation Speed platform 10, the centrifugal force of different size can be produced, thus the constant force of different size or the acting force of specific change in time can be produced to molecular sample.
Claims (8)
1. test single molecule force spectroscopy device based on centrifugal force for one kind, the microballoon comprising microscope and be connected with molecular sample one end, it is characterized in that, the other end of described molecular sample is connected with the front of molecule substrate, described molecule substrate transverse is fixed on above rotating mechanical arm, the rotating mechanical arm of described molecule substrate face is disposed with microscope, camera, photoelectricity rotary connector, electrical to optical converter and counterweight, the rotating mechanical arm of molecule substrate back is provided with light source, below described rotating mechanical arm, is provided with rotation platform.
2. one according to claim 1 tests single molecule force spectroscopy device based on centrifugal force, it is characterized in that, described microscopical object lens face microballoon and light source.
3. the one according to claim 1 and 2 tests single molecule force spectroscopy device based on centrifugal force, it is characterized in that, described microscope is optical microscope.
4. one according to claim 1 tests single molecule force spectroscopy device based on centrifugal force, it is characterized in that, the diameter of described microballoon is 1 micron to 25 microns.
5. the one according to claim 1 or 4 tests single molecule force spectroscopy device based on centrifugal force, it is characterized in that, described microballoon is made up of silicon ball, polystyrene microsphere or magnetic bead wherein one or more.
6. one according to claim 1 tests single molecule force spectroscopy device based on centrifugal force, it is characterized in that, described light source is LED.
7. one according to claim 1 tests single molecule force spectroscopy device based on centrifugal force, it is characterized in that, described camera is Ethernet camera.
8. one according to claim 1 tests single molecule force spectroscopy device based on centrifugal force, it is characterized in that, described molecule substrate is slide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410819884.3A CN104535570A (en) | 2014-12-24 | 2014-12-24 | Device for testing single molecule force spectroscopy based on centrifugal force |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410819884.3A CN104535570A (en) | 2014-12-24 | 2014-12-24 | Device for testing single molecule force spectroscopy based on centrifugal force |
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| CN104535570A true CN104535570A (en) | 2015-04-22 |
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| CN201410819884.3A Pending CN104535570A (en) | 2014-12-24 | 2014-12-24 | Device for testing single molecule force spectroscopy based on centrifugal force |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3383539A4 (en) * | 2015-12-01 | 2019-06-26 | Zebrasci, Inc. | High definition particle detection during centrifugation |
| CN111988586A (en) * | 2020-07-28 | 2020-11-24 | 中国兵器装备集团上海电控研究所 | Rotary test platform of rotary connector and optical transmitter and receiver |
Citations (4)
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| JP2003185544A (en) * | 2001-12-20 | 2003-07-03 | Ishikawajima Harima Heavy Ind Co Ltd | Method for manufacturing microscope observation sample mount in porous body |
| JP2004020444A (en) * | 2002-06-18 | 2004-01-22 | Tokyo Electric Power Co Inc:The | Wear particles capturing device, method of diagnosing lubrication object part, and lubrication object part diagnostic system |
| US20050260100A1 (en) * | 2002-04-13 | 2005-11-24 | Leif Robert C | Centrifugal cytology system, chamber block and method for the preparation of treated monolayers of sample material |
| US20100027110A1 (en) * | 2006-12-26 | 2010-02-04 | Nsk Ltd. | Observable centrifugal apparatus and observation apparatus |
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2014
- 2014-12-24 CN CN201410819884.3A patent/CN104535570A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003185544A (en) * | 2001-12-20 | 2003-07-03 | Ishikawajima Harima Heavy Ind Co Ltd | Method for manufacturing microscope observation sample mount in porous body |
| US20050260100A1 (en) * | 2002-04-13 | 2005-11-24 | Leif Robert C | Centrifugal cytology system, chamber block and method for the preparation of treated monolayers of sample material |
| JP2004020444A (en) * | 2002-06-18 | 2004-01-22 | Tokyo Electric Power Co Inc:The | Wear particles capturing device, method of diagnosing lubrication object part, and lubrication object part diagnostic system |
| US20100027110A1 (en) * | 2006-12-26 | 2010-02-04 | Nsk Ltd. | Observable centrifugal apparatus and observation apparatus |
Non-Patent Citations (1)
| Title |
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| KEN HALVORSEN, WESLEY P. WONG: "massively parallel single-molecule manipulation using centrifugal force", 《BIOPHYSICAL JOURNAL》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3383539A4 (en) * | 2015-12-01 | 2019-06-26 | Zebrasci, Inc. | High definition particle detection during centrifugation |
| CN111988586A (en) * | 2020-07-28 | 2020-11-24 | 中国兵器装备集团上海电控研究所 | Rotary test platform of rotary connector and optical transmitter and receiver |
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Application publication date: 20150422 |
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