CN104524000B - It is a kind of to improve improving eyesight rapid-released droppills of visual fatigue and preparation method thereof rapidly - Google Patents

It is a kind of to improve improving eyesight rapid-released droppills of visual fatigue and preparation method thereof rapidly Download PDF

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CN104524000B
CN104524000B CN201510030471.1A CN201510030471A CN104524000B CN 104524000 B CN104524000 B CN 104524000B CN 201510030471 A CN201510030471 A CN 201510030471A CN 104524000 B CN104524000 B CN 104524000B
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volume
dry extract
rapid
extract
dry
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CN104524000A (en
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卫强
李启照
杨春燕
华芳
李胜男
刘娟娟
吴甜甜
刘志
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Anhui Xinhua University
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    • A61K36/45—Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
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Abstract

The invention discloses a kind of improving eyesight rapid-released droppills that can improve visual fatigue rapidly and preparation method thereof, it is characterised in that：It is to process to obtain using matrimony vine, Radix Salviae Miltiorrhizae, pseudo-ginseng, cowberry, chrysanthemum, chrysanthemum leaf, Rhizoma Atractylodis Macrocephalae and Poria cocos as crude drug.The improving eyesight rapid-released droppills of the present invention are easy to use, and retinue's property is good, are adapted to have the different crowd of visual fatigue to use.

Description

It is a kind of to improve improving eyesight rapid-released droppills of visual fatigue and preparation method thereof rapidly
First, technical field
The present invention relates to a kind of improving eyesight rapid-released droppills that can improve visual fatigue rapidly and preparation method thereof, specifically one Kind triggers a kind of quick function medicament for improving visual fatigue with modern technology high efficiency extraction, purifying active ingredient with dripping pill.
2nd, background technology
With the popularization of computer, mobile phone and network technology, people constantly extend between using at the moment, intensity increases, visual fatigue Incidence increases year by year.Visual fatigue is also known as asthenopia, feels that uncomfortable is performance with eyes of patients, is occurred and essence with eye or whole body The syndrome that the related organic change of god, psychology is characterized, is mainly shown as：Discomfort or pain around eyeball and eye socket Feel, keep in dark place, shedding tears, eyes are dry and astringent etc., occur the constitutional symptoms such as headache, Nausea and vomiting, dizziness when serious, and normal spiritedness withers Waste, is drowsiness, failure of memory, the mental symptom such as insomnia.
The traditional Chinese medical science thinks visual fatigue based on the deficiency of liver-yin and kidney-yin, weakness of the spleen and the stomach, qi depression to blood stasis, and mistake eyesight, works with one's mind and overtax one's nerves It is then its predisposing factors." mesh is liver key ", " normal vision relying on sufficiency of liver-blood ".The Tang Dynasty SUN Si miao is write《Qianjin Yao Fang seven apertures door》In Say：" its reading, game etc. excessively use mesh person, name day hepatic asthenia." in addition, the traditional Chinese medical science thinks " the emotion of the spleen being anxiety ", " anxiety impairs the spleen." spleen residence Middle Jiao is mechanism of qi hinge, the mechanism of qi of main the whole body, because sitting makes mechanism of qi run not smooth, the disorder of qi renvoi dysfunction of the spleen in transport.It is modern more Medium technique is highly developed, and people are often staring to see computer, mobile phone, while with thinking, memory, reasoning, excitement, sorrow The thought such as worry, unhappy, disgruntled.Worry beyond measure, god is coagulated in thing, and mechanism of qi is detained not all right, then burnt taste mechanism of qi pivot in easily aggravating The burden of knob, injures spleen.《Ling Shu Miraculous Pivot or Divine Axis opinion puzzled greatly》Point out：" the essence of five ZANG-organs and six FU-organs gas, all upper note is in mesh." the essence of five ZANG-organs and six FU-organs Gas all relies temper and is able to uplink, if unsaturated vapor, it is impossible to above hold the foster witness of temperature, then it is dim regarding thing.
Improving eyesight rapid-released droppills medicine group becomes the fruit of Chinese wolfberry, pseudo-ginseng, Radix Salviae Miltiorrhizae, chrysanthemum, chrysanthemum leaf, cowberry, Poria cocos, Rhizoma Atractylodis Macrocephalae.Matrimony vine Son has nourishing liver and kidney, benefiting shrewd head effect.Modern research shows that wolfberry pigment and polysaccharide are its improving eyesight main components.Pseudo-ginseng Total saposins are pseudo-ginseng principle active component, have expansion blood vessel and coronary artery, reduce peripheral vascular resistance, increase cerebral blood flow (CBF) Acted on coronary flow, antithrombotic and anticoagulation etc..Radix Salviae Miltiorrhizae is important drugs promoting blood circulation and removing blood stasis, and so-called " Radix Salviae Miltiorrhizae dissipates simply, work( Same Siwu Tang ".Radix Salviae Miltiorrhizae China and Philippines quinones component has coronary artery dilator, increases coronary flow, improves microcirculation, improves blood stream Become state, the protection effect such as erythrocyte membrane and anti anoxia.Cowberry is Ericaceae cowberry platymiscium, and Europe is about from nineteen sixty-five It is used as eye health articles for use, its fruit procyanidins component has improvement eyesight, mitigation dizzy, promotes to regard red blood cell again It is raw, improve night vision, mitigate visual fatigue effect, it is mainly that procyanidine is acted on regarding Huang that it, which alleviates the visual fatigue mechanism of action, Aldehyde isomerase, adds the generating rate of the photoactive substance " rhodopsin " of retinal visual cell.When rhodopsin is subject to light It during stimulation of the line to retina, can be decomposed in moment, and the chemical change is transmitted to brain, produced " visible ".Rhizoma Atractylodis Macrocephalae is Feverfew,《Sheng Nong's herbal classic》Top grade is classified as, there is strengthening the spleen and replenishing qi, eliminate dampness and have diuretic effect.Poria cocos is On Polyporaceae Fu The dry sclerotia of Siberian cocklebur.《Compendium of Materia Medica》Say：" Poria cocos smell is light and oozes, its property uplink, born fluid, loosing the interspaces of skin and muscles ".Rhizoma Atractylodis Macrocephalae matches somebody with somebody Poria cocos, Rhizoma Atractylodis Macrocephalae invigorating the spleen, in relatively keeping, Poria cocos excreting dampness, relatively downlink, mutual reinforcement between makes temper transporting and improving eyesight effect to use.Modern study table Bright, atractylodes lactone and triterpenes components are Rhizoma Atractylodis Macrocephalae and the material base of Poria cocos strengthening the spleen and replenishing qi.
Pill is a kind of form of solid dispersions.Since it has, dissolution is fast, bioavilability is high, good effect, medicine The advantages that thing stability is good.Dripping pill is mostly sublingual administration, and medicine is directly absorbed into blood circulation by sublingual mucosa, avoids mouth The degraded loss of liver first-pass effect caused by clothes and medicine in stomach, makes high drug concentration reach target organ, 5-15 minutes fast Speed works.Rapid-released droppills are the atomic little crystal grains of the solid dispersion drug formed under quenching conditions, therefore are disintegrated fast, dissolution speed Degree is fast, has the characteristics that quick-acting, efficient.
3rd, the content of the invention
The present invention is intended to provide a kind of can improve medicine of visual fatigue and preparation method thereof rapidly, technology to be solved is asked Topic is to select suitable medicinal material to carry out compatibility, and active ingredient is obtained using modern times extraction, purification technique, using rapid-released droppills this The quick release dosage forms of kind, eye is released quickly against by medicine, rapid to improve visual fatigue symptom.
The present invention solves technical problem and adopts the following technical scheme that：
The present invention can improve rapidly the improving eyesight rapid-released droppills of visual fatigue by following mass parts crude drug extract with it is auxiliary Material processing obtains：
Wherein, auxiliary material used in the improving eyesight rapid-released droppills includes polyethylene glycol 2000, Macrogol 4000 and poloxamer 407, each auxiliary material is by the proportioning of mass parts：
3~4 parts of polyethylene glycol 2000；
4~5 parts of Macrogol 4000；
0.4-0.6 parts of poloxamer188.
Preferred mass part of each crude drug of improving eyesight rapid-released droppills of the present invention matches：
On the basis of preferred mass part of each crude drug, preferred mass part proportioning of each auxiliary material is：
3.5 parts of polyethylene glycol 2000；
4.5 parts of Macrogol 4000；
0.5 part of poloxamer188.
Improving eyesight rapid-released droppills of the present invention are prepared as follows to obtain：
1) take dried wolfberry, crushed 24 mesh sieves, add 3-5 times of volume by chloroform and ether by volume 1:1 is formed Mixed liquor, extracted 2-3 time, each 1-2min with flash extracter, merge extracting solution and simultaneously filter, it is molten in filtrate obtained by recycling It is after agent, the filtrate after removal solvent is dry to constant weight at 60 DEG C, dry extract a is obtained, gained filter cake adds 8-10 times of volume distillation Water continues to extract 3-4 times (voltage 70-90V) with flash extracter, each 2-3min, merges extracting solution and filters, gained filtrate 15-20g/L is concentrated into, then microfiltration membranes is crossed successively and ultrafiltration membrane (operating pressure 0.15-0.20MPa) obtains trapped fluid, will retain The 1/3-1/5 that liquid is concentrated into cumulative volume obtains concentrate, with n-butanol, chloroform and concentrate by volume 1：5：25 hybrid extractions remove Deproteinized matter 5-6 times, 60 DEG C of dryings of subnatant obtain dry extract b to constant weight.
2) dry Radix Salviae Miltiorrhizae and dry pseudo-ginseng are taken, co-grinding is crossed 100 mesh sieves, is put into apparatus,Soxhlet's, sequentially adds 10- The petroleum ether of 15 times of volumes, the aether backflow 4-6h of 10-15 times of volume discard petroleum ether liquid and ether solution, take out red to colourless Ginseng and pseudo-ginseng, 60 DEG C of dryings, the 90%-95% ethanol for adding 15-20 times of volume carry out 2-3 (voltage of homogenate extraction 100-120V), 2-3min is extracted every time, is merged extracting solution and is filtered, obtains filtrate A and filter cake A；Filter cake A adds 15-20 times of body Long-pending 65%-70% ethanol carries out homogenate extraction 2-3 times (voltage 100-120V), extracts 2-3min every time, merges extracting solution simultaneously Filter, obtain liquor B；Merging filtrate A and liquor B, recycling design, will remove 60 DEG C of dryings of mixing filtrate after solvent to constant weight, Obtain dry extract c；
3) take cowberry fresh fruit, add 10-15 times of volume by HCl and methanol that mass concentration is 2%-3% by volume 10:The mixed liquor that 90 mixing are formed carries out homogenate extraction, extracts 3-4 times (voltage 80-90V), extracts 1-2min every time, filters, Merging filtrate simultaneously crosses AB-8 type macroreticular resins, with water elution to colourless, then is eluted with methanol, 60 DEG C of dryings of meoh eluate to perseverance Weight, obtains dry extract d.
4) dry chrysanthemum and chrysanthemum leaf are taken, crushed 24 mesh sieves, is mixed, the 75%-80% ethanol for adding 10-15 times of volume dodges Formula extracts 3-4 times (voltage 90-100V), each 2-3min, merges extracting solution and filters, and gained filtrate is concentrated into 10-15g/L, Then microfiltration membranes and ultrafiltration membrane (operating pressure 0.20-0.22MPa) are crossed successively, discard trapped fluid, the dry drying of 60 DEG C of permeate To constant weight, dry extract e is obtained.
5) dry Rhizoma Atractylodis Macrocephalae and Poria cocos are taken, crushed 60 mesh sieves, is mixed, 30-40 times of addition presses volume by chloroform and ether Than 1:The 1 mixed liquid dipping 24-36h formed, with homogenate extraction 2-3 times (voltage 110-120V), each 3-4min, merges extraction Liquid simultaneously filters, and 60 DEG C of dryings of filtrate obtain dry extract f to constant weight.
6) taking polyethylene glycol 2000, Macrogol 4000, poloxamer188 melt in 70 DEG C of water-baths, add dry extract A, dry extract b, dry extract c, dry extract d, dry extract e and dry extract f, stir certain time, make its dispersed, in 80-85 DEG C insulation, is placed in dropping-pill machine liquid chamber and instills condensation pelletization (distance of a length of 60cm of condensation column, water dropper and condensate liquid is 6- 8cm, atoleine are cooling agent, and drop speed is 15-20 drops per minute, and condensate liquid tip temperature is 70-80 DEG C, and bottom temperature is 0- 3 DEG C), after dripping pill completely cooling, dripping pill is taken out, removes the atoleine of surface attachment, is put into vacuum drying chamber dry 24h To obtain the final product.
Compared with the prior art, beneficial effects of the present invention are embodied in：
The present invention is prepared into improving eyesight quick-release drop with the fruit of Chinese wolfberry, pseudo-ginseng, Radix Salviae Miltiorrhizae, chrysanthemum, chrysanthemum leaf, cowberry, Poria cocos, Rhizoma Atractylodis Macrocephalae prescription Ball.Wolfberry pigment, polysaccharide component in matrimony vine are obtained using homogenate extraction joint micro-filtration-ultrafiltration purification technology, using homogenate extraction- Macroreticular resin joint technology obtains cowberry procyanidins component, with homogenate extraction acquisition Radix Salviae Miltiorrhizae quinone, arasaponin, Rhizoma Atractylodis Macrocephalae Ester component and fuling triterpene constituents.Above component is added into polyethylene glycol 2000, Macrogol 4000, poloxamer188, Rapid-released droppills are made under quenching conditions.Rapid-released droppills are the atomic small crystalline substances of the solid dispersion drug formed under quenching conditions Grain, therefore disintegration is fast, dissolution rate is fast, can reach the rapid purpose for playing drug effect, has the characteristics that efficient, quick-acting.It is easy to use, with It is good from property, it is adapted to have the different crowd of visual fatigue to use.Further, since contain general flavone, lactone, phenanthrenequione constituents in prescription, Water is insoluble in, oral administration biaavailability is low, adds polyethylene glycol 2000, Macrogol 4000, poloxamer188 and above medicine Solid dispersions are made in active ingredient, form pill, can dramatically increase dissolution rate, improve the bioavilability in human body. Effect experiment shows that high, medium and low three dosage groups of improving eyesight rapid-released droppills can substantially reduce black eye rabbit and be damaged ciliary body tissue Middle cGMP concentration, raises its cAMP concentration, prompts to can obviously improve eye strain.It is blue or green few that human experimentation shows that said preparation can improve Year distant vision, duration of photopic vision, it is dry and astringent to significantly improve swollen eye caused by subject's asthenopia, ophthalmodynia, photophobia, blurred vision, eye Etc. symptom, no overt toxicity.
4th, embodiment
Embodiment 1：
1) dried wolfberry of 20 mass parts is taken, crushed 24 mesh sieves, adds the chloroform-ether (1 of 3 times of volumes：1, V/V), Extracted 2 times, each 2min with flash extracter, merge extracting solution and filter, it is dry at 60 DEG C after the solvent in recycling gained filtrate It is dry to constant weight, obtain dry extract a.Gained filter cake adds 8 times of volume distilled water to extract 3 times (voltage 90V) with flash extracter, every time 2min, merges extracting solution and filters, and gained filtrate is concentrated into 15g/L, then crosses microfiltration membranes and ultrafiltration membrane (operating pressure successively Trapped fluid 0.15MPa) is obtained, trapped fluid is concentrated into cumulative volume 1/4 obtains concentrate, with n-butanol-chloroform-concentrate (1：5： 25, V/V) extraction removes protein 5 times, and 60 DEG C of dryings of subnatant obtain dry extract b to constant weight.
2) the drying Radix Salviae Miltiorrhizae of 10 mass parts and the drying pseudo-ginseng of 10 mass parts are taken respectively, and co-grinding is crossed 100 mesh sieves, is put into In apparatus,Soxhlet's, the petroleum ether of 10 times of volumes, the aether backflow 6h of 10 times of volumes are sequentially added to colourless, discards petroleum ether liquid And ether solution, Radix Salviae Miltiorrhizae and pseudo-ginseng are taken out, 60 DEG C of dryings, it is (electric that 95% ethanol for adding 15 times of volumes carries out homogenate extraction 2 times Press 120V), 3min is extracted every time, is merged extracting solution and is filtered, obtains filtrate A and filter cake A；Filter cake A adds 15 times of volumes 70% ethanol carries out homogenate extraction 3 times (voltage 100V), extracts 2min every time, merges extracting solution and filters, obtains liquor B；Merge Filtrate A and liquor B, recycling design, 60 DEG C of dryings obtain dry extract c to constant weight；
3) the cowberry fresh fruit of 15 mass parts is taken, adds the 2%HCl- methanol (10 of 10 times of volumes：90) homogenate extraction is carried out, 3 times (voltage 90V) is extracted, extracts 1min every time, is filtered, merging filtrate crosses AB-8 type macroreticular resins, with water elution to colourless, then Eluted with methanol, 60 DEG C of dryings of meoh eluate obtain dry extract d to constant weight.
4) the drying chrysanthemum of 15 mass parts and the drying chrysanthemum leaf of 15 mass parts are taken, crushed 24 mesh sieves, is mixed, adds 10 times 75%% ethanol homogenate extraction 3 times (voltage 100V) of volume, each 3min, merges extracting solution and filters, the concentration of gained filtrate To 10g/L, microfiltration membranes and ultrafiltration membrane (operating pressure 0.20MPa) are then crossed successively, discards trapped fluid, 60 DEG C of dryings of permeate are extremely Constant weight, obtains dry extract e.
5) the drying Rhizoma Atractylodis Macrocephalae of 10 mass parts and the drying Poria cocos of 10 mass parts are taken, crushed 60 mesh sieves, is mixed, adds 30 times The chlorofonn-ethylacetate (1 of volume：1, V/V) 24h is soaked, with homogenate extraction 2 times (voltage 110V), each 4min, merges extraction Liquid simultaneously filters, and 60 DEG C of dryings of filtrate obtain dry extract f to constant weight.
6) take the pool Lip river of the polyethylene glycol 2000 of 3.5 mass parts, the Macrogol 4000 of 4.5 mass parts, 0.5 mass parts husky Nurse 407 melts in 70 DEG C of water-baths, adds dry extract a, dry extract b, dry extract c, dry extract d, dry extract e, dry extract f, stirs Certain time is mixed, makes its dispersed, in 80 DEG C of insulations, being placed in instillation condensation pelletization in dropping-pill machine liquid chamber, (condensation column is a length of The distance of 60cm, water dropper and condensate liquid is 8cm, and atoleine is cooling agent, and drop speed is dripped for per minute 15, condensate liquid tip temperature For 70 DEG C, bottom temperature is 0 DEG C), after dripping pill completely cooling, dripping pill is taken out, removes the atoleine of surface attachment, is put into true 24h is dried in empty drying box to obtain the final product.
【The test of pesticide effectiveness】
Test influence of the improving eyesight rapid-released droppills to cAMP, cGMP content in rabbit ciliary body tissue
Cyclic adenosine monophosphate (Cyclic Adenosine Monophosphate, cAMP), cyclic guanosine monophosphate (Cyclic Guanosine Monophosphate, cGMP) it is a pair of of Antagonism material, acted on by " second messenger ", adjust hormone, god Information transmission through mediator, participates in cell metabolism link.The former cAMP promotes fat and sugar member to decompose, and strengthens myocardial contraction, promotees Into smooth muscle relaxation and suppress the effect such as platelet aggregation, and the latter is then opposite.The two is to cell metabolism reaction and physiological function Adjustment effect on the contrary, concentration change is mutually related on the contrary.CAMP has important meaning to the formation for preventing eye fatigue, its is main By improving sarcoplasmic reticulum to Ca2+Intake, reduce sarcoplasm in Ca2+Concentration, causes cell relaxation.Can also be by stimulating smooth muscle The phosphorylation of myosin light chain kinase, prevents myofilament activation from suppressing contractile response.
1 materials and methods
1.1 experimental animal
2-2.5kg black eye rabbits, cleaning grade, is provided by Anhui University of Chinese Medecine's Experimental Animal Center.
1.2 experimental drug
The made improving eyesight rapid-released droppills of embodiment 1 (are made of) the fruit of Chinese wolfberry, chrysanthemum, cowberry, chrysanthemum leaf, Radix Salviae Miltiorrhizae, pseudo-ginseng, per ball weight 0.03g.Control medicine is that mulberry hemp pill is Chinese medicines quasi-word medicine, and Huangshan Tianmu Pharmaceutical Co., Ltd. produces, lot number 20130506.The medicine is made of mulberry leaf, Semen sesami nigrum (stir-fry), has nourishing the liver and kidney, dispelling pathogenic wind for improving eyesight effect.For kidney deficiency and liver, head Dizzy dim eyesight, blurring of vision, tearing against wind.Using the medicine as control, meet one of principle of positive control medicine setting, i.e., The quasi-font size Chinese patent drug or Western medicine of official approval.
1.3 experiment reagent
Rabbit cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) ELISA detection kit, are purchased from the peaceful biology of Shanghai discipline Scientific research Co., Ltd, lot number：130916.
1.4 key instrument
FSH-2 types are adjustable high speed refiner (Jiangsu wiener Tai Ke Instrument Ltd.), HH-S2 digital display thermostat water baths (Jin Cheng Guo Sheng laboratory apparatus factory of Jintan City), SK-1- eddy mixers (Jintan City into brightness instrument plant), LD24-1.2 centrifuges (system in Beijing Jing founds Co., Ltd), WD-9417B microplate reader (Hangzhou remittance that experimental instruments and equipment limited), FA2004 electronic balances (on Hai Minqiao precision scientific instruments Co., Ltd).
1.5 packets and model foundation
2-2.5kg health black eyes rabbit 60 is taken, is randomly divided into physiological saline group (physiological saline, 10mL/kg), model group (physiological saline, 10mL/kg), improving eyesight rapid-released droppills height (6.30g/kg), in (3.15g/kg), low (1.05g/kg) dosage group, Mulberry hemp pill (1.25g/kg) positive controls.Every group each 10, half male and half female.In addition to physiological saline group, other each groups are with daytime 30lux (illuminance), night 0lux carry out illumination, continuous illumination 10d.Continue to distinguish gastric infusion 2 weeks to the experimental rabbit after illumination, 2 times a day, it is 10mL/kg volume to be administered.
1.6 index determining
After last dose 24h, experimental rabbit is put to death with head-breaking, takes out bilateral eyeball, separates ciliary body, after rinsing well, Filter paper blots, and weighs to eyes ciliary body.It is homogenized 3~5 times, each 10s so that refiner is continuous in frozen water again, centrifuge 10~15min (low temperature low speed centrifuge 2000r/min), takes supernatant using detection shown in enzyme-linked immunosorbent assay (ELISA) reagent box CAMP and cGMP contents in method detection rabbit ciliary body.
1.7 data statistics
With SPSS17.0 software statistics, data carry out Cochran＆Cox approximations t inspections between group.
2 results
The change of cAMP and cGMP in rabbit ciliary body tissue, such as table 1.
Between 1 each group of table in ciliary body tissue cAMP and cGMP comparison (n=10)
Group cAMP(nmol/L) cGMP(nmol/L)
Physiological saline group 128.82±2.26 36.02±2.85
Model group 51.20±2.06 122.65±3.71
Improving eyesight rapid-released droppills high dose group 115.86±3.79**△ 39.22±1.90*△
Improving eyesight rapid-released droppills middle dose group 90.97±2.92**△ 50.18±3.62**△
Improving eyesight rapid-released droppills low dose group 73.95±3.61**△ 62.06±2.84**△
Mulberry hemp pill 89.11±2.74** 66.19±3.79**△
Note：* represent compared with physiological saline group there is conspicuousness (P<0.05)；* is represented compared with physiological saline group, there is pole Conspicuousness (P<0.01)；△Expression has pole conspicuousness (P compared with model group<0.01)
As shown in Table 1, due to the influence of long-time illumination, excessively with eye, make ciliary muscle that spastic contractions, model group eyelash occur CAMP is decreased obviously compared with physiological saline group in shape body tissue, and cGMP is significantly raised compared with physiological saline group, prompts eyes to be damaged seriously. Compared with model group, high, medium and low three dosage groups of improving eyesight rapid-released droppills can make to be damaged in ciliary body tissue in model group Significantly raised (the P of cAMP<0.01).For the cAMP concentration being increased to, improving eyesight rapid-released droppills are high, middle dose group is better than mulberry hemp pill Positive controls (89.11nmol/L).Compared with model group, high, medium and low three dosage groups of improving eyesight rapid-released droppills can make mould CGMP in ciliary body tissue is damaged in type group substantially reduces (P<0.01), in terms of the cGMP concentration being reduced to, improving eyesight rapid-released droppills High, medium and low three dosage groups are superior to mulberry hemp pill positive controls (66.19nmol/L).
Testing two improving eyesight rapid-released droppills improves teenagers eyes fatigue experiment
1.1 experimental drug
The made improving eyesight rapid-released droppills of embodiment 1 are (by the fruit of Chinese wolfberry, chrysanthemum, cowberry, chrysanthemum leaf, Radix Salviae Miltiorrhizae, pseudo-ginseng, Rhizoma Atractylodis Macrocephalae, Poria cocos group Into), per ball 0.30g.
1.2 key instrument
Urinate ten analyzers；MXFA6100 type blood counting instruments；Triangle disposition solid block diagram for legibility measure； E word standard near vision charts；Ophthalmoscope；GB standard visual chart；Slit-lamp；Trial lens set.
1.3 method
1.3.1 study subject selection criteria
18~35 years old age computer work person 40, excludes following study subject：Infectious, traumatic eye part disease； With cornea, crystal, vitreum, eyeground pathological changes person；There is the disease patient such as cardiovascular and cerebrovascular, liver, kidney, hemopoietic system；Take with it is tested The related medicine of function, influences result judgement person；The long-term use of medicine in relation to treating eyesight, health products use other treatment Method fails terminator etc..
1.3.2 experiment packet is with compareing
Test-meal group and placebo group, every group of each 20 people are set up in this experiment, and experiment is used between itself cross-reference and group Control.1.3.3 experimental method
Amount is given according to recommended amounts, test-meal group takes orally improving eyesight rapid-released droppills, 3 times a day, and 1 tablet each time, control group mouth Take color, the character placebo identical with Bilberry fruit P.E improving eyesight rapid-released droppills, the same test-meal group of amount, duration of trial 7d.
1.3.4 Testing index and project
Indices are respectively measured once before and after experiment.Testing index and project have：General inspection (including subjective sense Feel, sleep state, mental status), (physique, blood, urine, feces are conventional, blood parameters, B ultrasound, chest x-ray and the heart for constitution inspection Electrograph), examination of eyes (subjective symptoms；Eye is swollen, ophthalmodynia, photophobia, blurred vision, the dry and astringent situation of eye).
Visuognosis persistence test formula：The photopic vision time/watch total time × 100%, minute 2min attentively, measure 2 times It is averaged.Eye test uses national standard eyesight table look-up.Examination of near vision uses international near vision test type inspection.
By mild symptoms, in, score again, test front and rear statistics integrated value, severe meter 3 divides, moderate meter 2 divides, slightly counts 1 Point.It is effective that each symptom, which improves 1 point, and it is obvious to improve 2 points, calculates symptom improvement rate.
1.3.5 data processing and result judgement
With SPSS17.0 software statistics, data carry out Cochran＆Cox approximations t inspections between group.
Result judgement：Test-meal group itself compares after test-meal experiment and test-meal group is with compareing comparison among groups, symptom total mark, bright It is obviously improved depending on persistence and total effective rate, difference has conspicuousness and average duration of photopic vision improves and is more than or equal to 0.1, it is believed that has Effect：Not up to effective standard to be invalid.Eyesight：Uncorrected visual acuity is significantly improved, and (uncorrected visual acuity improves 0.2 or eyesight table look-up Improve and more than two rows think effective, eyesight is improved thinks invalid less than two rows), this index for reference only.Medicine group with it is right Comparing according to group has statistical significant difference, it is believed that having improves the health-care effect of visual fatigue.
2 results
2.1 before experiment relatively
40 subjects, measure height, weight, are randomly divided into each 20 of medicine group, control group before testing.Medicine group male 12, women 8, average age 21.2 ± 1.0 years old, average height and weight be respectively 169.0 ± 5.6cm and 62.4 ± 6.1kg；Control group male 15, women 5, average age 20.8 ± 1.6 years old, average height and weight be respectively 169.1 ± 5.3cm and 62.8 ± 4.9kg, through statistical test no significant difference (P>0.05).As shown in table 2, it is the photopic vision time between two groups, naked Eye distant vision and symptom integral do not have significant difference (P yet>0.05).
Table 2 observe before ordinary circumstance compare (n=20,)
2.2 distant visions improve situation
As shown in table 3, for control group compared with before experiment, distant vision improves no significant improvement (P>0.05).Medicine group Compared with before experiment, left eye has significant improvement (P<0.05), right eye pole significant improvement (P<0.01)；Medicine group and control group Compare, left eye significant improvement (P is also presented<0.05), right eye pole significant improvement (P<0.01).
Before and after 3 two groups of experiments of table distant vision situation compare (n=20,)
Note：* represent there is conspicuousness (P compared with before testing with group<0.05)；* is represented with having pole compared with before testing with group Conspicuousness (P<0.01)；△Expression has conspicuousness (P compared with control group<0.05)；△△Expression has pole conspicuousness compared with control group (P<0.01)
2.3 durations of photopic vision improve situation
For medicine group compared with before experiment, duration of photopic vision has pole significant improvement (P<0.01)；Medicine group and control group phase Than duration of photopic vision, which is also presented, pole significant improvement (P<0.01).It is shown in Table 4.
Before and after 4 two groups of experiments of table duration of photopic vision change comparison (n=20,)
Group Before experiment After experiment Improve percentage/%
Positive group 51.79±9.65 68.12±6.11** 31.53
Control group 52.84±8.02 51.98±9.21 -1.63
Note：* represents there is pole conspicuousness (P compared with before testing with group<0.01),△△Expression has extremely aobvious compared with control group Work property (P<0.01)
2.4 symptoms improve situation
Medicine group subject eye is swollen, ophthalmodynia, photophobia, blurred vision, the dry and astringent improvement rate of eye are significantly higher than control group.The result is shown in Table 5, table 6.
5 medicine group cardinal symptom of table improves situation
Symptom Case load It is effective Effectively It is invalid Improvement rate/%
Eye is swollen 19 6 10 3 84.21
Ophthalmodynia 17 4 8 5 70.59
Photophobia 14 3 7 4 71.43
Blurred vision 16 5 8 3 81.25
It is dry and astringent 16 8 6 2 87.50
6 control group cardinal symptom of table improves situation
Symptom Case load It is effective Effectively It is invalid Improvement rate/%
Eye is swollen 15 0 1 14 6.67
Ophthalmodynia 14 0 2 12 14.29
Photophobia 16 1 2 13 18.75
Blurred vision 17 1 1 15 11.76
It is dry and astringent 14 1 2 11 21.43
2.5 symptom integrals count
Clinical symptoms integration has significant difference (P before and after medicine group experiment<0.05), reduced rate 81.21%；And compare Clinical symptoms integration there was no significant difference (P before and after group experiment>0.05), reduced rate is only 8.07%, medicine group and control group phase Than clinical symptoms integration has significant difference (P<0.001).It is shown in Table 7.
7 clinical symptoms of table integration statistics (n=20,)
Group Before experiment After experiment Reduced rate/%
Medicine group 2.82±2.80 0.56±1.25**△△ 81.21
Control group 2.85±2.01 2.62±1.32 8.07
Note：* represents there is pole conspicuousness (P compared with before testing with group<0.01),△△Expression has extremely aobvious compared with control group Work property (P<0.01)
2.6 safety indexes are observed
Security inspection results contrast before and after test-meal, is shown in Table 8.Subject's B ultrasound, chest x-ray and ECG examination before test-meal No abnormality seen.General physical examination, blood parameters and the blood of two groups of crowds, urine, feces routine inspection result before and after test-meal Substantially in normal range (NR).
8 security inspection results contrast of table (n=20,)
Embodiment 2：
1) dried wolfberry of 20 mass parts is taken, crushed 24 mesh sieves, adds the chloroform-ether (1 of 4 times of volumes：1, V/V), Extracted 3 times, each 1min with flash extracter, merge extracting solution and filter, it is dry at 60 DEG C after the solvent in recycling gained filtrate It is dry to constant weight, obtain dry extract a.Gained filter cake adds 9 times of volume distilled water to extract 3 times (voltage 80V) with flash extracter, every time 3min, merges extracting solution and filters, and gained filtrate is concentrated into 18g/L, then crosses microfiltration membranes and ultrafiltration membrane (operating pressure successively Trapped fluid 0.17MPa) is obtained, trapped fluid is concentrated into cumulative volume 1/3 obtains concentrate, with n-butanol-chloroform-concentrate (1：5： 25, V/V) extraction removes protein 5 times, and 60 DEG C of dryings of subnatant obtain dry extract b to constant weight.
2) the drying Radix Salviae Miltiorrhizae of 10 mass parts and the drying pseudo-ginseng of 10 mass parts are taken respectively, and co-grinding is crossed 100 mesh sieves, is put into In apparatus,Soxhlet's, the petroleum ether of 12 times of volumes, the aether backflow 5h of 12 times of volumes are sequentially added to colourless, discards petroleum ether liquid And ether solution, Radix Salviae Miltiorrhizae and pseudo-ginseng, 60 DEG C of dryings are taken out, 95% ethanol for sequentially adding 15 times of volumes carries out homogenate extraction 3 Secondary (voltage 110V), extracts 3min every time, merges extracting solution and filters, obtains filtrate A and filter cake A；Filter cake A adds 15 times of volumes 65% ethanol carry out homogenate extraction 2 times (voltage 110V), extract 3min every time, merge extracting solution and simultaneously filter, obtain liquor B；Close And filtrate A and liquor B, recycling design, 60 DEG C of dryings obtain dry extract c to constant weight；
3) the cowberry fresh fruit of 15 mass parts is taken, adds the 3%HCl- methanol (10 of 12 times of volumes：90) homogenate extraction is carried out, 4 times (voltage 80V) is extracted, extracts 1min every time, is filtered, merging filtrate crosses AB-8 type macroreticular resins, with water elution to colourless, then Eluted with methanol, 60 DEG C of dryings of meoh eluate obtain dry extract d to constant weight.
4) the drying chrysanthemum of 15 mass parts and the drying chrysanthemum leaf of 15 mass parts are taken, crushed 24 mesh sieves, is mixed, adds 15 times 80% ethanol homogenate extraction 4 times (voltage 95V) of volume, each 2min, merges extracting solution and filters, gained filtrate is concentrated into 12g/L, then crosses microfiltration membranes and ultrafiltration membrane (operating pressure 0.21MPa), discards trapped fluid, the dry drying of 60 DEG C of permeate successively To constant weight, dry extract e is obtained.
5) the drying Rhizoma Atractylodis Macrocephalae of 10 mass parts and the drying Poria cocos of 10 mass parts are taken, crushed 60 mesh sieves, is mixed, adds 30- The chlorofonn-ethylacetate (1 of 40 times of volumes：1, V/V) 28h is soaked, with homogenate extraction 3 times (voltage 110V), each 3min, takes out Filter, 60 DEG C of filtrate is dry to constant weight, obtains dry extract f.
6) take the pool Lip river of the polyethylene glycol 2000 of 3.5 mass parts, the Macrogol 4000 of 4.5 mass parts, 0.5 mass parts husky Nurse 407 melts in 70 DEG C of water-baths, adds dry extract a, dry extract b, dry extract c, dry extract d, dry extract e, dry extract f, stirs Certain time is mixed, makes its dispersed, in 85 DEG C of insulations, being placed in instillation condensation pelletization in dropping-pill machine liquid chamber, (condensation column is a length of The distance of 60cm, water dropper and condensate liquid is 6cm, and atoleine is cooling agent, and drop speed is dripped for per minute 16, condensate liquid tip temperature For 75 DEG C, bottom temperature is 2 DEG C), after dripping pill completely cooling, dripping pill is taken out, removes the atoleine of surface attachment, is put into true 24h is dried in empty drying box to obtain the final product.
Improving eyesight rapid-released droppills are same as Example 1 through efficacy testing obtained by the present embodiment.
Embodiment 3：
1) dried wolfberry of 20 mass parts is taken, crushed 24 mesh sieves, adds the chloroform-ether (1 of 5 times of volumes：1, V/V), Extracted 3 times, each 1min with flash extracter, merge extracting solution and filter, it is dry at 60 DEG C after the solvent in recycling gained filtrate It is dry to constant weight, obtain dry extract a.Gained filter cake adds 10 times of volume distilled water to extract 4 times (voltage 70V) with flash extracter, often Secondary 3min, merges extracting solution and filters, and gained filtrate is concentrated into 20g/L, then crosses microfiltration membranes and ultrafiltration membrane (operating pressure successively Trapped fluid 0.20MPa) is obtained, trapped fluid is concentrated into cumulative volume 1/5 obtains concentrate, with n-butanol-chloroform-concentrate (1：5： 25, V/V) extraction removes protein 6 times, and 60 DEG C of dryings of subnatant obtain dry extract b to constant weight.
2) the drying Radix Salviae Miltiorrhizae of 10 mass parts and the drying pseudo-ginseng of 10 mass parts are taken respectively, and co-grinding is crossed 100 mesh sieves, is put into In apparatus,Soxhlet's, the petroleum ether of 10 times of volumes, the aether backflow 6h of 10 times of volumes are sequentially added to colourless, discards petroleum ether liquid And ether solution, Radix Salviae Miltiorrhizae and pseudo-ginseng, 60 DEG C of dryings are taken out, 90% ethanol for sequentially adding 20 times of volumes carries out homogenate extraction 3 Secondary (voltage 100V), extracts 2min every time, merges extracting solution and filters, obtains filtrate A and filter cake A；Filter cake A adds 20 times of volumes 70% ethanol carry out homogenate extraction 2 times (voltage 120V), extract 3min every time, merge extracting solution and simultaneously filter, obtain liquor B；Close And filtrate A and liquor B, recycling design, 60 DEG C of dryings obtain dry extract c to constant weight；
3) the cowberry fresh fruit of 15 mass parts is taken, adds the 3%HCl- methanol (10 of 15 times of volumes：90) homogenate extraction is carried out, 4 times (voltage 80V) is extracted, extracts 1min every time, is filtered, merging filtrate crosses AB-8 type macroreticular resins, with water elution to colourless, Eluted with methanol, 60 DEG C of dryings of meoh eluate obtain dry extract d to constant weight.
4) the drying chrysanthemum of 15 mass parts and the drying chrysanthemum leaf of 15 mass parts are taken, crushed 24 mesh sieves, is mixed, adds 15 times 75% ethanol homogenate extraction 4 times (voltage 90V) of volume, each 3min, merges extracting solution and filters, gained filtrate is concentrated into 15g/L, then crosses microfiltration membranes and ultrafiltration membrane (operating pressure 0.22MPa), discards trapped fluid, the dry drying of 60 DEG C of permeate successively To constant weight, dry extract e is obtained.
5) the drying Rhizoma Atractylodis Macrocephalae of 10 mass parts and the drying Poria cocos of 10 mass parts are taken, crushed 60 mesh sieves, is mixed, adds 40 times The chlorofonn-ethylacetate (1 of volume：1, V/V) 36h is soaked, with homogenate extraction 2 times (voltage 120V), each 4min, filters, filter 60 DEG C of liquid is dry to constant weight, obtains dry extract f.
6) take the pool Lip river of the polyethylene glycol 2000 of 3.5 mass parts, the Macrogol 4000 of 4.5 mass parts, 0.5 mass parts husky Nurse 407 melts in 70 DEG C of water-baths, adds dry extract a, dry extract b, dry extract c, dry extract d, dry extract e, dry extract f, stirs Certain time is mixed, makes its dispersed, in 82 DEG C of insulations, being placed in instillation condensation pelletization in dropping-pill machine liquid chamber, (condensation column is a length of The distance of 60cm, water dropper and condensate liquid is 7cm, and atoleine is cooling agent, and drop speed is dripped for per minute 20, condensate liquid tip temperature For 80 DEG C, bottom temperature is 3 DEG C), after dripping pill completely cooling, dripping pill is taken out, removes the atoleine of surface attachment, is put into true 24h is dried in empty drying box to obtain the final product.
Improving eyesight rapid-released droppills are same as Example 1 through efficacy testing obtained by the present embodiment.
Embodiment 4
1) dried wolfberry of 18 mass parts is taken, crushed 24 mesh sieves, adds the chloroform-ether (1 of 3 times of volumes：1, V/V), Extracted 2 times, each 3min with flash extracter, merge extracting solution and filter, it is dry at 60 DEG C after the solvent in recycling gained filtrate It is dry to constant weight, obtain dry extract a.Gained filter cake adds 8 times of volume distilled water to extract 3 times (voltage 90V) with flash extracter, every time 2min, merges extracting solution and filters, and gained filtrate is concentrated into 16g/L, crosses microfiltration membranes and ultrafiltration membrane (operating pressure respectively Trapped fluid 0.15MPa) is obtained, trapped fluid is concentrated into cumulative volume 1/4 obtains concentrate, with n-butanol-chloroform-concentrate (1：5： 25, V/V) extraction removes protein 5 times, and 60 DEG C of dryings of subnatant obtain dry extract b to constant weight.
2) the drying Radix Salviae Miltiorrhizae of 8 mass parts and the drying pseudo-ginseng of 8 mass parts are taken respectively, and co-grinding crosses 100 mesh sieves, is put into rope In family name's extractor, sequentially add the petroleum ether of 10 times of volumes, the aether backflow 4h of 10 times of volumes to colourless, discard petroleum ether liquid and Ether solution, takes out Radix Salviae Miltiorrhizae and pseudo-ginseng, 60 DEG C of dryings, sequentially adds 95% ethanol row homogenate extraction, 2 (electricity of 15 times of volumes Press 100V), 2min is extracted every time, is merged extracting solution and is filtered, obtains filtrate A and filter cake A；Filter cake A adds 15 times of volumes 70% ethanol carries out homogenate extraction 3 times (voltage 110V), extracts 2min every time, merges extracting solution and filters, obtains liquor B；Merge Filtrate A and liquor B, recycling design, 60 DEG C of dryings obtain dry extract c to constant weight；
3) the cowberry fresh fruit of 13 mass parts is taken, adds the 2%HCl- methanol (10 of 10 times of volumes：90) homogenate extraction is carried out, 3 times (voltage 80V) is extracted, extracts 2min every time, is filtered, merging filtrate crosses AB-8 type macroreticular resins, with water elution to colourless, Eluted with methanol, 60 DEG C of dryings of meoh eluate obtain dry extract d to constant weight.
4) the drying chrysanthemum of 13 mass parts and the drying chrysanthemum leaf of 13 mass parts are taken, crushed 24 mesh sieves, is mixed, adds 10 times 80% ethanol homogenate extraction 4 times (voltage 90V) of volume, each 2min, merges extracting solution and filters, gained filtrate is concentrated into 10g/L, then crosses microfiltration membranes and ultrafiltration membrane (operating pressure 0.22MPa), discards trapped fluid, the dry drying of 60 DEG C of permeate successively To constant weight, dry extract e is obtained.
5) the drying Rhizoma Atractylodis Macrocephalae of 8 mass parts and the drying Poria cocos of 8 mass parts are taken, crushed 60 mesh sieves, is mixed, adds 30 times of bodies Long-pending chlorofonn-ethylacetate (1：1, V/V) 24h is soaked, with homogenate extraction 3 times (voltage 110V), each 3min, filters, filtrate 60 DEG C dry to constant weight, obtains dry extract f.
6) polyethylene glycol 2000 of 3 mass parts, the Macrogol 4000 of 4 mass parts, the poloxamer of 0.4 mass parts are taken 407 melt in 70 DEG C of water-baths, add dry extract a, dry extract b, dry extract c, dry extract d, dry extract e, dry extract f, stirring Certain time, makes its dispersed, and in 84 DEG C of insulations, being placed in instillation condensation pelletization in dropping-pill machine liquid chamber, (condensation column is a length of The distance of 60cm, water dropper and condensate liquid is 7cm, and atoleine is cooling agent, and drop speed is dripped for per minute 15, condensate liquid tip temperature For 70-80 DEG C, bottom temperature is 1 DEG C), after dripping pill completely cooling, dripping pill is taken out, the atoleine of surface attachment is removed, puts Enter in vacuum drying chamber and dry 24h to obtain the final product.
Improving eyesight rapid-released droppills are same as Example 1 through efficacy testing obtained by the present embodiment.
Embodiment 5
1) dried wolfberry of 22 mass parts is taken, crushed 24 mesh sieves, adds the chloroform-ether (1 of 3 times of volumes：1, V/V), Extracted 3 times, each 2min with flash extracter, merge extracting solution and filter, it is dry at 60 DEG C after the solvent in recycling gained filtrate It is dry to constant weight, obtain dry extract a.Gained filter cake adds 8 times of volume distilled water to extract 3 times (voltage 90V) with flash extracter, every time 2min, merges extracting solution and filters, and gained filtrate is concentrated into 15g/L, then crosses microfiltration membranes and ultrafiltration membrane (operating pressure successively Trapped fluid 0.20MPa) is obtained, trapped fluid is concentrated into cumulative volume 1/5 obtains concentrate, with n-butanol-chloroform-concentrate (1：5： 25, V/V) extraction removes protein 6 times, and 60 DEG C of dryings of subnatant obtain dry extract b to constant weight.
2) the drying Radix Salviae Miltiorrhizae of 12 mass parts and the drying pseudo-ginseng of 12 mass parts are taken respectively, and co-grinding is crossed 100 mesh sieves, is put into In apparatus,Soxhlet's, the petroleum ether of 15 times of volumes, the aether backflow 6h of 15 times of volumes are sequentially added to colourless, discards petroleum ether liquid And ether solution, Radix Salviae Miltiorrhizae and pseudo-ginseng, 60 DEG C of dryings are taken out, 95% ethanol for sequentially adding 20 times of volumes carries out homogenate extraction 3 Secondary (voltage 120V), extracts 3min every time, merges extracting solution and filters, obtains filtrate A and filter cake A；Filter cake A adds 20 times of volumes 65% ethanol carry out homogenate extraction 2 times (voltage 120V), extract 2min every time, merge extracting solution and simultaneously filter, obtain liquor B；Close And filtrate A and liquor B, recycling design, 60 DEG C of dryings obtain dry extract c to constant weight；
3) the cowberry fresh fruit of 17 mass parts is taken, adds the 3%HCl- methanol (10 of 15 times of volumes：90) homogenate extraction is carried out, 4 times (voltage 90V) is extracted, extracts 2min every time, is filtered, merging filtrate crosses AB-8 type macroreticular resins, with water elution to colourless, Eluted with methanol, 60 DEG C of dryings of meoh eluate obtain dry extract d to constant weight.
4) the drying chrysanthemum of 17 mass parts and the drying chrysanthemum leaf of 17 mass parts are taken, crushed 24 mesh sieves, is mixed, adds 15 times 80% ethanol homogenate extraction 4 times (voltage 100V) of volume, each 3min, merges extracting solution and filters, gained filtrate is concentrated into 15g/L, then crosses microfiltration membranes and ultrafiltration membrane (operating pressure 0.22MPa), discards trapped fluid, the dry drying of 60 DEG C of permeate successively To constant weight, dry extract e is obtained.
5) the drying Rhizoma Atractylodis Macrocephalae of 12 mass parts and the drying Poria cocos of 12 mass parts are taken, crushed 60 mesh sieves, is mixed, adds 40 times The chlorofonn-ethylacetate (1 of volume：1, V/V) 36h is soaked, with homogenate extraction 3 times (voltage 120V), each 4min, filters, filter 60 DEG C of liquid is dry to constant weight, obtains dry extract f.
6) polyethylene glycol 2000 of 4 mass parts, the Macrogol 4000 of 5 mass parts, the poloxamer of 0.6 mass parts are taken 407 melt in 70 DEG C of water-baths, add dry extract a, dry extract b, dry extract c, dry extract d, dry extract e, dry extract f, stirring Certain time, makes its dispersed, and in 82 DEG C of insulations, being placed in instillation condensation pelletization in dropping-pill machine liquid chamber, (condensation column is a length of The distance of 60cm, water dropper and condensate liquid is 6cm, and atoleine is cooling agent, and drop speed is dripped for per minute 16, condensate liquid tip temperature For 80 DEG C, bottom temperature is 0 DEG C), after dripping pill completely cooling, dripping pill is taken out, removes the atoleine of surface attachment, is put into true 24h is dried in empty drying box to obtain the final product.
Improving eyesight rapid-released droppills are same as Example 1 through efficacy testing obtained by the present embodiment.

Claims (2)

  1. A kind of 1. preparation method for the improving eyesight rapid-released droppills that can improve visual fatigue rapidly, it is characterised in that：
    The improving eyesight rapid-released droppills are obtained by the extract of the crude drug of following mass parts with auxiliary material processing：
    Auxiliary material used in the improving eyesight rapid-released droppills includes polyethylene glycol 2000, Macrogol 4000 and poloxamer188, each auxiliary material It is by the proportioning of mass parts：
    3~4 parts of polyethylene glycol 2000；
    4~5 parts of Macrogol 4000；
    0.4-0.6 parts of poloxamer188；
    The preparation method of the improving eyesight rapid-released droppills is to operate according to the following steps：
    1) dried wolfberry, crushed 24 mesh sieves, add 3-5 times of volume by chloroform and ether by volume 1:1 mixing formed Liquid, is extracted 2-3 times, each 1-2min with flash extracter, is merged extracting solution and is filtered, the solvent in recycling gained filtrate, 60 DEG C of dryings obtain dry extract a to constant weight, and gained filter cake adds 8-10 times of volume distilled water to continue to extract 3-4 times with flash extracter, Each 2-3min, merges extracting solution and filters, and gained filtrate is concentrated into 15-20g/L, then crosses microfiltration membranes successively and ultrafiltration membrane obtains Trapped fluid, the 1/3-1/5 that trapped fluid is concentrated into cumulative volume obtain concentrate, with n-butanol, chloroform and concentrate by volume 1： 5：25 hybrid extractions remove protein 5-6 times, and 60 DEG C of dryings of subnatant obtain dry extract b to constant weight；
    2) dry Radix Salviae Miltiorrhizae and dry pseudo-ginseng are taken, co-grinding is crossed 100 mesh sieves, is put into apparatus,Soxhlet's, sequentially adds 10-15 times The petroleum ether of volume, the aether backflow 4-6h of 10-15 times of volume discard petroleum ether liquid and ether solution to colourless, take out Radix Salviae Miltiorrhizae and Pseudo-ginseng, 60 DEG C of dryings, the 90%-95% ethanol for adding 15-20 times of volume carry out homogenate extraction 2-3 times, extract 2- every time 3min, merges extracting solution and filters, obtain filtrate A and filter cake A；Filter cake A add the 65%-70% ethanol of 15-20 times of volume into Row homogenate extraction 2-3 times, extracts 2-3min every time, merges extracting solution and filters, obtains liquor B；Merging filtrate A and liquor B, recycling Solvent, 60 DEG C of dryings obtain dry extract c to constant weight；
    3) take cowberry fresh fruit, add 10-15 times of volume by HCl and methanol that mass concentration is 2%-3% by volume 10:90 The mixed liquor that mixing is formed carries out homogenate extraction, extracts 3-4 times, extracts 1-2min every time, filters, and merging filtrate simultaneously crosses AB-8 types Macroreticular resin, with water elution to colourless, then with methanol elutes to obtain meoh eluate, by 60 DEG C of dryings of meoh eluate to constant weight, Obtain dry extract d；
    4) dry chrysanthemum and chrysanthemum leaf are taken, crushed 24 mesh sieves, is mixed, the 75%-80% ethanol of 10-15 times of volume of addition is flash to be carried Take 3-4 times, each 2-3min, merge extracting solution simultaneously filter, gained filtrate is concentrated into 10-15g/L, then successively cross microfiltration membranes and Ultrafiltration membrane, discards trapped fluid, and 60 DEG C of dryings of permeate obtain dry extract e to constant weight；
    5) dry Rhizoma Atractylodis Macrocephalae and Poria cocos are taken, crushed 60 mesh sieves, is mixed, 30-40 times of volume of addition presses volume by chloroform and ether Than 1:The 1 mixed liquid dipping 24-36h formed, with homogenate extraction 2-3 time, each 3-4min, merging extracting solution simultaneously filters, filtrate 60 DEG C of dryings obtain dry extract f to constant weight；
    6) taking polyethylene glycol 2000, Macrogol 4000, poloxamer188 melt in 70 DEG C of water-baths, add dry extract a, do Medicinal extract b, dry extract c, dry extract d, dry extract e and dry extract f, stir evenly, and in 80-85 DEG C of insulation, are placed in pill dripping machine dropping liquid Indoor instillation condenses pelletization, after dripping pill completely cooling, takes out dripping pill, removes the atoleine of surface attachment, be put into vacuum and do 24h is dried in dry case to obtain the final product.
  2. 2. preparation method according to claim 1, it is characterised in that：The improving eyesight rapid-released droppills by following mass parts original The extract of medicinal material is obtained with auxiliary material processing：
    Auxiliary material used in the improving eyesight rapid-released droppills includes polyethylene glycol 2000, Macrogol 4000 and poloxamer188, each auxiliary material It is by the proportioning of mass parts：
    3.5 parts of polyethylene glycol 2000；
    4.5 parts of Macrogol 4000；
    0.5 part of poloxamer188.
CN201510030471.1A 2015-01-21 2015-01-21 It is a kind of to improve improving eyesight rapid-released droppills of visual fatigue and preparation method thereof rapidly Expired - Fee Related CN104524000B (en)

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CN108853307A (en) * 2018-10-10 2018-11-23 赵翔 A kind of Chinese materia medica preparation for treating insufficiency of natural endowment type long sight
CN111700952A (en) * 2020-07-06 2020-09-25 右江民族医学院 A Chinese medicinal quick-release dripping pill for treating venomous snake bite, and its preparation method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1178682A (en) * 1996-10-07 1998-04-15 郭书文 Chinese drug Mingmudan pill for improving eyesight and its producing method
CN1660226A (en) * 2004-12-29 2005-08-31 王四旺 'Wuzi Mingmu' pill and preparing technique
CN101347244A (en) * 2008-09-22 2009-01-21 曲建波 Health beverage
CN101406596A (en) * 2008-11-18 2009-04-15 李金伟 Medicament for treating dry eye
CN102091214A (en) * 2011-03-02 2011-06-15 朱宝民 Medicament for improving eyesight

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1178682A (en) * 1996-10-07 1998-04-15 郭书文 Chinese drug Mingmudan pill for improving eyesight and its producing method
CN1660226A (en) * 2004-12-29 2005-08-31 王四旺 'Wuzi Mingmu' pill and preparing technique
CN101347244A (en) * 2008-09-22 2009-01-21 曲建波 Health beverage
CN101406596A (en) * 2008-11-18 2009-04-15 李金伟 Medicament for treating dry eye
CN102091214A (en) * 2011-03-02 2011-06-15 朱宝民 Medicament for improving eyesight

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