CN104523802A - Method for extracting staphylococcus aureus inhibitor from shepherd's purse - Google Patents
Method for extracting staphylococcus aureus inhibitor from shepherd's purse Download PDFInfo
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- CN104523802A CN104523802A CN201410804868.7A CN201410804868A CN104523802A CN 104523802 A CN104523802 A CN 104523802A CN 201410804868 A CN201410804868 A CN 201410804868A CN 104523802 A CN104523802 A CN 104523802A
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Abstract
The invention discloses a method for extracting a staphylococcus aureus inhibitor from shepherd's purse. The staphylococcus aureus inhibitor is extracted by taking 95% of ethanol as an extracting agent under the assistance of ultrasonic wave, in an extracting solution, HCl concentration is 5-6% and material-to-liquid ratio is 1 to (9-11), extracting duration is 50-70min, and the extracting solution is concentrated and dried so as to prepare the staphylococcus aureus inhibitor. The diameter of a staphylococcus aureus inhibition zone reaches 34-35mm when the extract concentration is 20-25mg/mL, which is 20-30% bigger than that of a shepherd's purse extract inhibiting staphylococcus aureus inhibition zone prepared by virtue of a normal method, so that the inhibitor disclosed by the invention is quite significant in inhibiting effect. The method disclosed by the invention is simple, low in cost and strong in practicability, and the obtained shepherd's purse extract has a good application prospect in the aspects of medical and healthcare foods.
Description
Technical field
The present invention relates to a kind of method extracting staphylococcus aureus mortifier from Herba Capsellae.
Background technology
Herba Capsellae is Cruciferae 1 year or 2 years raw self-pollinated plants, and also known as ground dish, blazon temperate zone, the Southern Hemisphere and subtropical zone, all there is distribution China various places.Spring food Herba Capsellae is China's long-standing traditional custom among the people, and have saying of " to March three, ground dish can working as miracle ", cook thick soup with Herba Capsellae, taste is very good, both can increase appetite, can whet the appetite again and increase the longevity, therefore also known as " thick soup at the age of one hundred years old ".Traditional Chinese Medicine thinks that Herba Capsellae nature and flavor are sweet flat nontoxic, has strengthening the spleen improving eyesight, dispels the wind antipyretic, effect of hemostasis stomach function regulating, can be used for dysentery, edema, spits blood, has blood in stool, the control of the various diseases such as gonorrhea, chyluria, menorrhagia, conjunctival congestion and pain of eye.
Research shows, Herba Capsellae extract has inhibitory action to mouse entity tumor, Herba Capsellae is containing ergocristine, there is the effect of similar ergotin, decocting liquid oozes out in early days inflammation and has certain inhibitory action, and Herba Capsellae crude polysaccharides has certain inhibition to escherichia coli, bacillus subtilis, staphylococcus aureus, Salmonella.In visible Herba Capsellae all may there is fungistatic effect in many kinds of substance, also may there is many kinds of substance and work in coordination with depression effect.
Staphylococcus aureus is a kind of important pathogen of the mankind, is under the jurisdiction of staphylococcus, has the another name of " addicted to meat bacterium ", can cause many severe infections.Therefore, from Herba Capsellae, extract staphylococcus aureus mortifier can be widely used in medicine and field of health care food, significant.But how making Herba Capsellae extract have the effect of optimal inhibition staphylococcus aureus, is but the technical barrier never solved.
Summary of the invention
For the Herba Capsellae extract of the staphylococcus aureus optimum efficiency that is inhibited also reduces costs as far as possible, make it the condition possessing industrialized implementation, the present inventor is through lot of experiments, adopt response surface curve method and by the mode of Modling model, finally a kind of method extracting staphylococcus aureus mortifier from Herba Capsellae is invented, the method is simple, feasibility is strong, the Herba Capsellae extract concentrations obtained suppresses staphylococcus aureus effective when 20-25mg/mL, reach 34-35mm to the inhibition zone of staphylococcus aureus.
For obtaining the Herba Capsellae extract suppressing staphylococcus aureus optimum efficiency, the present invention adopts ultrasonic assistant to extract Herba Capsellae antibacterial substance, show that ethanol is on the basis of optimum extraction agent in single factor test optimization, the parameter had a significant impact by tool in Minnimum Run Equireplicated Res IV experimental design screening Extraction technique is HCl concentration, solid-liquid ratio and extraction time, and then the central point of response phase method optimization experiment is determined by steepest climbing experiment, according to the central combination design principle of Box-Behnken, design four factor three level experiments, establish ternary quadratic regression equation, through the checking to model, this model better can predict Herba Capsellae extract fungistatic effect.
By model prediction and lot of experiments checking, the technical scheme extracting staphylococcus aureus mortifier from Herba Capsellae that the present invention finally obtains is: with 95% ethanol for extractant, employing ultrasonic assistant extracts, in extracting solution, HCl concentration is 5%-6%, solid-liquid ratio is 1:9-1:11, extraction time is 50-70 minute, and extracting solution is concentrated, after drying and get final product.
The Herba Capsellae extract obtained by said method dissolves, adopt Odontothrips loti to investigate and suppress staphylococcus aureus effect, to extract reagent solution for blank, parallel laboratory test three times, after cultivating 24-48h at flat board being placed in 28 DEG C, observe and measure the diameter of transparent circle.
Extract concentrations of the present invention reaches 34-35 mm when 20-25mg/mL to the antibacterial circle diameter of staphylococcus aureus, and the Herba Capsellae extract obtained than commonsense method suppresses the large 20%-30% of staphylococcus aureus loop diameter, and fungistatic effect is very outstanding.
Compared with prior art, beneficial effect of the present invention is:
By Modling model and lot of experiments, finally a kind of method extracting staphylococcus aureus mortifier from Herba Capsellae has been invented, promote scientific and technological progress effectively, Herba Capsellae extract fungistatic effect prepared by the method is very outstanding, extract concentrations its antibacterial circle diameter when 20-25mg/mL reaches 34-35 mm, the Herba Capsellae extract obtained than commonsense method suppresses the large 20%-30% of staphylococcus aureus loop diameter, the inventive method is simple, cost is low, practical, the Herba Capsellae extract obtained medicine and health food in there is good application prospect.
Detailed description of the invention
Below a kind of method extracting staphylococcus aureus mortifier from Herba Capsellae is described in detail.
embodiment 1:
With 95% ethanol for extractant, adopt ultrasonic assistant to extract, in extracting solution, HCl concentration is 5.11%, and solid-liquid ratio is 1:9.3, and extraction time is 61 minutes, and extracting solution is concentrated, after drying and get final product.
Extract concentrations of the present invention is 34.47mm when 22.7mg/mL to the antibacterial circle diameter of staphylococcus aureus, and fungistatic effect is very outstanding.
embodiment 2:
With 95% ethanol for extractant, adopt ultrasonic assistant to extract, in extracting solution, HCl concentration is 5.93%, and solid-liquid ratio is 1:10.2, and extraction time is 51 minutes, and extracting solution is concentrated, after drying and get final product.
Extract concentrations of the present invention is 34.89mm when 24.6mg/mL to the antibacterial circle diameter of staphylococcus aureus, and fungistatic effect is very outstanding.
embodiment 3:
With 95% ethanol for extractant, adopt ultrasonic assistant to extract, in extracting solution, HCl concentration is 5.51%, and solid-liquid ratio is 1:10.8, and extraction time is 68 minutes, and extracting solution is concentrated, after drying and get final product.
Extract concentrations of the present invention is 34.06mm when 20.9mg/mL to the antibacterial circle diameter of staphylococcus aureus, and fungistatic effect is very outstanding.
Above-described embodiment not does any pro forma restriction to the present invention, any those of ordinary skill in the art, when not departing from technical solution of the present invention scope, the technology contents of above-mentioned announcement all can be utilized to make many possible variations and modification to technical solution of the present invention, or be revised as the Equivalent embodiments of equivalent variations.Therefore, every content not departing from technical solution of the present invention, according to the technology of the present invention essence to any simple modification made for any of the above embodiments, equivalent variations and modification, all should drop in the scope of technical solution of the present invention protection.
Claims (2)
1. one kind is extracted the method for staphylococcus aureus mortifier from Herba Capsellae, it is characterized in that: the ethanol with 95% is extractant, employing ultrasonic assistant extracts, in extracting solution, HCl concentration is 5%-6%, solid-liquid ratio is 1:9-1:11, extraction time is 50-70 minute, and extracting solution is concentrated, after drying and get final product.
2. the Herba Capsellae extract that obtains of method according to claim 1, is characterized in that: extract concentrations reaches 34-35 mm when 20-25mg/mL to the antibacterial circle diameter of staphylococcus aureus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410804868.7A CN104523802B (en) | 2014-12-23 | 2014-12-23 | A kind of method that staphylococcus aureus mortifier is extracted from shepherd's purse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410804868.7A CN104523802B (en) | 2014-12-23 | 2014-12-23 | A kind of method that staphylococcus aureus mortifier is extracted from shepherd's purse |
Publications (2)
| Publication Number | Publication Date |
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| CN104523802A true CN104523802A (en) | 2015-04-22 |
| CN104523802B CN104523802B (en) | 2017-08-18 |
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| CN201410804868.7A Expired - Fee Related CN104523802B (en) | 2014-12-23 | 2014-12-23 | A kind of method that staphylococcus aureus mortifier is extracted from shepherd's purse |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103584253A (en) * | 2013-11-13 | 2014-02-19 | 湖南科技大学 | Natural preservative with leaf mustard leaves and camphor tree leaves used as raw materials, as well as preparation method and application of natural preservative |
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2014
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103584253A (en) * | 2013-11-13 | 2014-02-19 | 湖南科技大学 | Natural preservative with leaf mustard leaves and camphor tree leaves used as raw materials, as well as preparation method and application of natural preservative |
Non-Patent Citations (6)
| Title |
|---|
| 刘春风: "荠菜中总生物碱提取工艺研究", 《化学工业与工程》 * |
| 康秀荣等: "蒙药荠菜的研究进展", 《中国民族医药杂志》 * |
| 李艳辉等: "超声波辅助提取荠菜总黄酮工艺研究", 《江苏农业科学》 * |
| 杨咏杰等: "荠菜多糖的超声波提取工艺及其抑菌活性的研究", 《食品工业科技》 * |
| 潘明: "超声波强化提取荠菜中总生物碱的研究", 《化学与生物工程》 * |
| 许瑞波等: "荠菜总生物碱的酸碱提取工艺研究", 《食品与发酵工业》 * |
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| CN104523802B (en) | 2017-08-18 |
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Granted publication date: 20170818 Termination date: 20171223 |