CN104523755A - Cockroach effective part and preparation method and application thereof - Google Patents
Cockroach effective part and preparation method and application thereof Download PDFInfo
- Publication number
- CN104523755A CN104523755A CN201410524173.3A CN201410524173A CN104523755A CN 104523755 A CN104523755 A CN 104523755A CN 201410524173 A CN201410524173 A CN 201410524173A CN 104523755 A CN104523755 A CN 104523755A
- Authority
- CN
- China
- Prior art keywords
- cockroach
- effective site
- defat
- preparation
- extractum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001674044 Blattodea Species 0.000 title claims abstract description 80
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000000284 extract Substances 0.000 claims abstract description 30
- 239000003814 drug Substances 0.000 claims abstract description 29
- 208000025157 Oral disease Diseases 0.000 claims abstract description 16
- 208000030194 mouth disease Diseases 0.000 claims abstract description 16
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 14
- 208000007117 Oral Ulcer Diseases 0.000 claims abstract description 11
- 208000002399 aphthous stomatitis Diseases 0.000 claims abstract description 11
- 201000001245 periodontitis Diseases 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 9
- 206010018275 Gingival atrophy Diseases 0.000 claims abstract description 8
- 201000005562 gingival recession Diseases 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 8
- 239000000126 substance Substances 0.000 claims abstract description 6
- 230000002265 prevention Effects 0.000 claims abstract description 5
- 230000000474 nursing effect Effects 0.000 claims abstract description 4
- 238000004440 column chromatography Methods 0.000 claims abstract 3
- 238000005238 degreasing Methods 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 32
- 239000000706 filtrate Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 15
- 229910052799 carbon Inorganic materials 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 8
- 229940079593 drug Drugs 0.000 claims description 8
- 239000012046 mixed solvent Substances 0.000 claims description 8
- 230000034994 death Effects 0.000 claims description 7
- 239000003480 eluent Substances 0.000 claims description 7
- 238000010992 reflux Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 208000007565 gingivitis Diseases 0.000 claims description 5
- 238000010521 absorption reaction Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000003826 tablet Substances 0.000 claims description 4
- 239000002023 wood Substances 0.000 claims description 4
- 238000010828 elution Methods 0.000 claims description 3
- 235000014593 oils and fats Nutrition 0.000 claims description 3
- -1 reflux Substances 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 239000002671 adjuvant Substances 0.000 claims description 2
- 239000000443 aerosol Substances 0.000 claims description 2
- 238000009835 boiling Methods 0.000 claims description 2
- 229940046011 buccal tablet Drugs 0.000 claims description 2
- 239000006189 buccal tablet Substances 0.000 claims description 2
- 239000007938 effervescent tablet Substances 0.000 claims description 2
- 239000000499 gel Substances 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 239000000865 liniment Substances 0.000 claims description 2
- 229940040145 liniment Drugs 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000002674 ointment Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims 3
- 239000013527 degreasing agent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 206010061218 Inflammation Diseases 0.000 abstract description 8
- 230000004054 inflammatory process Effects 0.000 abstract description 5
- 108010015899 Glycopeptides Proteins 0.000 abstract description 4
- 102000002068 Glycopeptides Human genes 0.000 abstract description 4
- 230000010261 cell growth Effects 0.000 abstract description 4
- DQJCDTNMLBYVAY-ZXXIYAEKSA-N (2S,5R,10R,13R)-16-{[(2R,3S,4R,5R)-3-{[(2S,3R,4R,5S,6R)-3-acetamido-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-5-(ethylamino)-6-hydroxy-2-(hydroxymethyl)oxan-4-yl]oxy}-5-(4-aminobutyl)-10-carbamoyl-2,13-dimethyl-4,7,12,15-tetraoxo-3,6,11,14-tetraazaheptadecan-1-oic acid Chemical compound NCCCC[C@H](C(=O)N[C@@H](C)C(O)=O)NC(=O)CC[C@H](C(N)=O)NC(=O)[C@@H](C)NC(=O)C(C)O[C@@H]1[C@@H](NCC)C(O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O)[C@@H](CO)O1 DQJCDTNMLBYVAY-ZXXIYAEKSA-N 0.000 abstract description 3
- 206010030113 Oedema Diseases 0.000 abstract description 3
- 102000014150 Interferons Human genes 0.000 abstract description 2
- 108010050904 Interferons Proteins 0.000 abstract description 2
- 102000015696 Interleukins Human genes 0.000 abstract description 2
- 108010063738 Interleukins Proteins 0.000 abstract description 2
- 206010057249 Phagocytosis Diseases 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 210000002919 epithelial cell Anatomy 0.000 abstract description 2
- 229940079322 interferon Drugs 0.000 abstract description 2
- 150000002617 leukotrienes Chemical class 0.000 abstract description 2
- 210000002540 macrophage Anatomy 0.000 abstract description 2
- 230000008782 phagocytosis Effects 0.000 abstract description 2
- 229920005862 polyol Polymers 0.000 abstract 2
- 150000003077 polyols Chemical class 0.000 abstract 2
- 230000001737 promoting effect Effects 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 108010066486 EGF Family of Proteins Proteins 0.000 abstract 1
- 102000018386 EGF Family of Proteins Human genes 0.000 abstract 1
- 108050007372 Fibroblast Growth Factor Proteins 0.000 abstract 1
- 102000018233 Fibroblast Growth Factor Human genes 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 230000000740 bleeding effect Effects 0.000 abstract 1
- 230000007969 cellular immunity Effects 0.000 abstract 1
- 229940047122 interleukins Drugs 0.000 abstract 1
- 244000052769 pathogen Species 0.000 abstract 1
- 238000007670 refining Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 26
- 238000012360 testing method Methods 0.000 description 14
- 239000000047 product Substances 0.000 description 9
- 208000025865 Ulcer Diseases 0.000 description 8
- 239000013558 reference substance Substances 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 231100000397 ulcer Toxicity 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 210000004195 gingiva Anatomy 0.000 description 6
- 210000004185 liver Anatomy 0.000 description 6
- 210000000214 mouth Anatomy 0.000 description 6
- 241000283973 Oryctolagus cuniculus Species 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 230000035764 nutrition Effects 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 231100000111 LD50 Toxicity 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000003304 gavage Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000012827 research and development Methods 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 206010053615 Thermal burn Diseases 0.000 description 3
- 229940023032 activated charcoal Drugs 0.000 description 3
- 210000001909 alveolar process Anatomy 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 210000000981 epithelium Anatomy 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000002547 new drug Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000009290 xinmailong Substances 0.000 description 3
- 206010002091 Anaesthesia Diseases 0.000 description 2
- 208000006386 Bone Resorption Diseases 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 2
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 208000005888 Periodontal Pocket Diseases 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 230000037005 anaesthesia Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000024279 bone resorption Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 239000000469 ethanolic extract Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 230000002440 hepatic effect Effects 0.000 description 2
- 206010020718 hyperplasia Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012567 medical material Substances 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 230000003239 periodontal effect Effects 0.000 description 2
- 238000005498 polishing Methods 0.000 description 2
- 239000008227 sterile water for injection Substances 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 2
- 241000238659 Blatta Species 0.000 description 1
- 241000238660 Blattidae Species 0.000 description 1
- 206010007558 Cardiac failure chronic Diseases 0.000 description 1
- 208000000419 Chronic Hepatitis B Diseases 0.000 description 1
- 241000219109 Citrullus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 201000006306 Cor pulmonale Diseases 0.000 description 1
- 206010010970 Cor pulmonale chronic Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- 241000500891 Insecta Species 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000238661 Periplaneta Species 0.000 description 1
- 241000238675 Periplaneta americana Species 0.000 description 1
- 241000337665 Phatnoma Species 0.000 description 1
- 208000033240 Progressive symmetric erythrokeratodermia Diseases 0.000 description 1
- 208000004186 Pulmonary Heart Disease Diseases 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 208000026636 chronic pulmonary heart disease Diseases 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 208000000718 duodenal ulcer Diseases 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 229960001412 pentobarbital Drugs 0.000 description 1
- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 1
- WRLGYAWRGXKSKG-UHFFFAOYSA-M phenobarbital sodium Chemical compound [Na+].C=1C=CC=CC=1C1(CC)C(=O)NC([O-])=NC1=O WRLGYAWRGXKSKG-UHFFFAOYSA-M 0.000 description 1
- 230000008288 physiological mechanism Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 210000003240 portal vein Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 208000008128 pulmonary tuberculosis Diseases 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to a cockroach effective part, a preparation method and an application thereof. The extract mainly comprises cockroach biological active peptide, glycopeptides, amino acids, and polyol, wherein cockroach biological active peptide has the function of imitating epidermal growth factors and basic fibroblast growth factors, and has the effects of promoting oral epithelial cell growth, resisting bacteria, eliminating inflammation, stopping bleeding, relieving edema, nourishing gum, and the like. the glycopeptide component has the function of activating non-specific cellular immunity, enhances direct phagocytosis of pathogens by macrophages and natural cells, secretes substances similar to interleukins, interferon leukotriene, and the like, relieves inflammation and edema; polyol has the functions of removing free radicals, and promoting cell growth. The cockroach effective part of the invention is prepared from cockroach dry bodies through extraction, degreasing, column chromatography, concentration and refining. The cockroach effective part of the invention has good prevention and improvement effects on various oral diseases such as common oral ulcer, periodontitis, gingival atrophy and the like, and can be used for preparing medicaments or nursing materials for treating the diseases.
Description
Technical field
The present invention relates to medical science and oral care techniques field, specifically, the present invention relates to and a kind of there is cockroach effective site of control multiple oral disease effect and its preparation method and application.
Background technology
Cockroach (Periplaneta americana) belongs to Insecta, Blattaria, Blattidae, is commonly called as Blatta seu periplaneta.It has been survived 400,000,000 years on earth, be in the world vitality the most by force, the most ancient, multiply one of the most successful insect assembly so far.Cockroach is considered as insect by the mankind always, but the various means of killing of the mankind faced by it can indomitablely be multiplied so far, have classic bioactive substance and special physiological mechanism in its body undoubtedly.
In recent years, along with the attention that China develops Chinese Traditional Medicine the Study on Resources, scientist has carried out the research and development of application aspect to this resourceful insecticide, achieve gratifying achievements, and with cockroach medical material for raw material, successively the new drug of research and development has: " Kangfuxin Liquid ", " the grand capsule of liver ", " Xinmailong injection ", wherein " the grand capsule of liver " and " Xinmailong injection " is national two kind new medicines (Chinese medicine)." Kangfuxin Liquid " is used for the treatment of oral ulcer, duodenal ulcer, burn, scald, decubital ulcer, pulmonary tuberculosis auxiliary treatment etc., is national Chinese medicine protection kind, national medical insurance Class B medicine; " the grand capsule of liver " is used for the treatment of chronic hepatitis B, the hepatic disease such as liver cirrhosis and HBV carrier, and total effective rate reaches more than 84.2%; " Xinmailong injection " cures mainly chronic heart failure, chronic pulmonary heart disease, obvious especially to Secondary cases pulmonary heart disease, myocardiac heart failure therapeutic effect.
Research in recent years shows, cockroach contains biologically active peptide, glycopeptide and multicomponent alcoholics compound, wherein cockroach peptide has the effect of plan epithelical cell growth factor (EGF) and basic fibroblast growth factor (bFGF), can promote the effects such as mouth epithelial cells growth, anti-inflammation, hemostasia and detumescence, nutrition gingiva; Glycopeptide constituents has activation Nonspecific immunity function, and enhancing macrophage and nature cell are to the direct phagocytosis of cause of disease material, and secrete the materials such as class interleukin, interferon leukotriene, diminish inflammation edema; Polyhydric alcohol has scavenging free radicals, the effect of Promote cell's growth.For having the active site of anti-inflammation, hemostasia and detumescence, promotion blood vessel hyperplasia, tissue repair effect in clear and definite cockroach medical material, understand fully the material base wherein played a major role further, inventor expands detailed pharmaceutical procedures research to it.Inventor adopts the alcohol extraction of variable concentrations, extracting method adopts the conventional merceration of commercial production or heat to put forward two kinds of methods, subtractive process selects price active carbon that is lower, that be easy to repeated regeneration utilization to study cockroach extractive, and carry out tracking activity in conjunction with experiment in vivo and vitro, obtain the active site with anti-inflammation, hemostasia and detumescence, promotion blood vessel hyperplasia, tissue repair effect.
Summary of the invention
The object of the present invention is to provide a kind of be suitable for suitability for industrialized production, can efficient solution to determine the cockroach effective site of the multiple oral diseases such as oral ulcer, periodontitis, gingival atrophy.
Another object of the present invention is to provide a kind of preparation method with the cockroach effective site of the multiple oral disease effects such as prevention and therapy oral ulcer, gingivitis, periodontitis, gingival atrophy.
Another object of the present invention is to provide a kind of medicine, drug regimen or oral care product containing cockroach effective site.
The invention provides a kind of preparation method of cockroach effective site, comprise the following steps:
By boiling hot dead with the hot water of 50-60 DEG C for the adult of culturing time more than 5 months, after drying through 60 ~ 70 DEG C, be crushed to 10-30 order, put in extraction pot, add the alcohol water mixed solvent that 3 ~ 7 times amount concentration are 60 ~ 95%, stir evenly, adopt reflux, extract, or merceration to extract, be 200 ~ 500 object filter-cloth filterings by extracting solution aperture, collect filtrate, through 50-80 DEG C of concentrating under reduced pressure, to extractum without alcohol taste, now relative density is 1.1-1.3, obtains cockroach alcohol extract.
60-75 DEG C of hot water of extractum and extractum volume 2-5 times amount is added in oily-water seperating equipment, slowly stir along a direction, mixing speed 20-40 rev/min, mixing time 20-30 minute, be heated to 65-75 DEG C simultaneously, be incubated 1 hour, leave standstill cooling 6-12 hour, after oil-water separation, release water layer, filter, collect filtrate, obtain cockroach defat extracting solution.
By activated-charcoal column on above-mentioned cockroach defat extracting solution, absorption 2-3 hour, with the alcohol water mixed solvent eluting of 50-95%, collects eluent.
Filtered by above-mentioned eluent, filtrate is at 50-80 DEG C of concentrating under reduced pressure, and being concentrated into relative density is 1.10-1.30, obtains cockroach effective part extract.
Described extracting method.Reflux, extract: extract three times, 3 ~ 5 hours first times, second and third time 2 ~ 4 hours, reflux temperature controls at 70 ~ 80 DEG C.Merceration extracts: extract three times, and each immersion more than 72 hours, stirred once at interval of 6 ~ 12 hours therebetween.
In described alcohol water mixed solvent, alcohol comprises methanol, ethanol, ethylene glycol or their mixture.
Described active carbon is wood substance grain shaped activated carbon, and the consumption of active carbon is the 2-5 times amount of defat extracting liquid volume.
The present invention also provides one can solve the multiple oral diseases such as oral ulcer, periodontitis, gingival atrophy, for the preparation for the treatment of and prevention oral disease medicine cockroach effective site, total peptide content, in bSA, adopts Folin-phenol method to measure, must not be less than 100.0mg/g.
The present invention also provides a kind of cockroach effective site as preparation treatment and the application preventing oral disease medicine.
Described cockroach effective site is above-mentioned cockroach effective site.。
Described medicine comprises medicine, drug regimen and oral care product.
Described oral disease comprises oral ulcer, gingivitis, periodontitis and gingival atrophy.
Described medicine, drug regimen and nursing materials comprise the various adjuvants tablet, membrane, liniment, ointment, gel, aerosol, spray and the gargarism made that add pharmaceutics and allow.
Described tablet comprises buccal tablet, effervescent tablet and mouth paster.
Beneficial effect of the present invention: cockroach aboundresources, realizes artificial breeding at present completely, national annual output reaches more than 600 ton.The research and development of cockroach medicine series are one of five large series " cloud medicine " distinguishing products of Yunnan Province's emphasis cultivation, the natural product that the research achievement obtains, by for research and development safety, effectively, aboundresources, the new drug with the multiple oral disease such as control and treatment oral ulcer, gingivitis, periodontitis, gingival atrophy and daily oral care product lay the foundation, and makes this resourceful pharmaceutical insects better for the mankind serve.
Specific embodiment
Hereafter describe embodiments of the invention in detail.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they mutually can be combined and be combined with each other thus reach better technique effect.
Embodiment one: cockroach urgees the preparation method of oral cavity tissue reparation and nutrition gingiva activity extract
1 extracts: choose the cockroach adult that culturing time is more than 5 months, scalds after death 60 ~ 70 DEG C of oven dry with the hot water of 50 ~ 60 DEG C.Dry for cockroach adult is crushed to 10 ~ 30 orders, put in steeping tank, adding 5 times amount volume fractions is the alcohol reflux of 60 ~ 95%, Extracting temperature controls at 70 ~ 80 DEG C, first time extracts 3 hours, second and third time is extracted 2 hours, is then 200 ~ 500 object filter-cloth filterings by extracting solution aperture, collects filtrate.
2 concentrate: by above-mentioned filtrate reduced in volume, temperature is 50 ~ 70 DEG C, and be concentrated into extractum without alcohol taste, now relative density is 1.05 ~ 1.20, obtains cockroach ethanol extract.
3 defats: the hot water adding 3 times amount 60 ~ 75 DEG C of fluid extract and extractum volume in oily-water seperating equipment, slowly stir along a direction, mixing speed is 30 ~ 50 revs/min, be heated to 65 ~ 75 DEG C simultaneously, be incubated 1 hour, leave standstill cooling 12 hours, after oils and fats is separated, releases water layer, with 200 ~ 500 order filter-cloth filterings, collect filtrate, filtrate reduced in volume, temperature is 55 ~ 65 DEG C, being concentrated into relative density is 1.1 ~ 1.3, obtains cockroach defat extract.
4 refine: by activated-charcoal column on cockroach defat extracting solution, and adsorb 2 ~ 3 hours, and with the ethanol elution of 60%, collect eluent, filter, filtrate reduced in volume, temperature is 60 DEG C, and being concentrated into relative density is 1.10 ~ 1.30, obtains cockroach extractive of the present invention.
Active carbon is wood substance grain shaped activated carbon, and the consumption of active carbon is 2 times amount volumes of defat extracting solution.
5 measure: the mensuration of total peptide in extractum, and adopt the total peptide content in Folin-phenol method mensuration cockroach extractive, this product in bSA, must not be less than 100.0mg/g containing total peptide.
The preparation bSA reference substance 0.2g of 5.1 reference substance solution, accurately weighed, put in 100ml measuring bottle, add water to scale, shake up.Precision measures 10ml, is placed in 100ml measuring bottle, adds water to scale, shake up, and obtains (every 1ml is containing bSA 200 μ g).
5.2 standard curves prepare precision measure reference substance solution 0.00,0.10,0.20,0.40,0.60,0.80,1.00ml in 10ml measuring bottle, adding distil water polishing is to 1.00ml.Often manage and add alkaline copper reagent (containing 0.5mol/L NaOH, 10%Na
2cO
3, 0.1%NaKC
4h
4o
6and 0.05%CuSO
4) 1.0ml, mixing.35 DEG C of reacting by heating 10min, cooling; Add Folin-phenol reagent 4.0ml successively fast again, mixing, 55 DEG C of reacting by heating 15min, cooling, add water standardize solution, with reference substance solution 0.00 μ g/ml concentration for blank, measures absorbance according to ultraviolet visible spectrophotometry at 760nm wavelength place, take absorbance as vertical coordinate, concentration is abscissa drawing standard curve.
5.3 algoscopys get this product 1.0g, accurately weighed, add appropriate distilled water ultrasonic dissolution, are settled to 100ml, obtain need testing solution with water.Precision pipettes need testing solution 1.0ml, and the method preparation under the preparation of sighting target directrix curve, measures absorbance in accordance with the law, and calculate the content of total peptide in need testing solution with standard curve.
Embodiment two: cockroach urgees the preparation method of oral cavity tissue reparation and nutrition gingiva activity extract
1 extracts: choose the cockroach adult that culturing time is more than 5 months, scalds after death 60 ~ 70 DEG C of oven dry with the hot water of 50 ~ 60 DEG C.Dry for cockroach adult is crushed to 10 ~ 30 orders, and put in steeping tank, the ethanol merceration adding 3 ~ 5 times amount 60 ~ 95% extracts three times, each soak time is more than 72 hours, stirring once at interval of 6 ~ 12 hours therebetween, is 200 ~ 500 object filter-cloth filterings with aperture, merging filtrate.
2 concentrate: by above-mentioned filtrate reduced in volume, temperature is 50 ~ 70 DEG C, and be concentrated into extractum without alcohol taste, now relative density is 1.05 ~ 1.20, obtains cockroach ethanol extract.
3 defats: the hot water adding 3 times amount 60 ~ 75 DEG C of fluid extract and extractum volume in oily-water seperating equipment, slowly stir along a direction, mixing speed is 30 ~ 50 revs/min, be heated to 65 ~ 75 DEG C simultaneously, be incubated 1 hour, leave standstill cooling 12 hours, after oils and fats is separated, releases water layer, with 200 ~ 500 order filter-cloth filterings, collect filtrate, filtrate reduced in volume, temperature is 50 ~ 70 DEG C, being concentrated into relative density is 1.10 ~ 1.30, obtains cockroach defat extract.
4 refine: by activated-charcoal column on cockroach defat extracting solution, and adsorb 2 ~ 3 hours, with the ethanol elution of 60%, collect eluent, filter, filtrate reduced in volume, temperature is 50 ~ 70 DEG C, and being concentrated into relative density is 1.10 ~ 1.30, obtains cockroach extractive of the present invention.
Active carbon is wood substance grain shaped activated carbon, and the consumption of active carbon is 2 times amount volumes of defat extracting solution.
5 measure: the mensuration of total peptide in extractum, and adopt the total peptide content in Folin-phenol method mensuration cockroach extractive, this product in bSA, must not be less than 100.0mg/g containing total peptide.
The preparation bSA reference substance 0.2g of 5.1 reference substance solution, accurately weighed, put in 100ml measuring bottle, add water to scale, shake up.Precision measures 10ml, is placed in 100ml measuring bottle, adds water to scale, shake up, and obtains (every 1ml is containing bSA 200 μ g).
5.2 standard curves prepare precision measure reference substance solution 0.00,0.10,0.20,0.40,0.60,0.80,1.00ml in 10ml measuring bottle, adding distil water polishing is to 1.00ml.Often manage and add alkaline copper reagent (containing 0.5mol/L NaOH, 10%Na2CO3,0.1%NaKC4H4O6 and 0.05%CuSO4) 1.0ml, mixing.35 DEG C of reacting by heating 10min, cooling; Add Folin-phenol reagent 4.0ml successively fast again, mixing, 55 DEG C of reacting by heating 15min, cooling, add water standardize solution, with reference substance solution 0.00 μ g/ml concentration for blank, measures absorbance according to ultraviolet visible spectrophotometry at 760nm wavelength place, take absorbance as vertical coordinate, concentration is abscissa drawing standard curve.
5.3 algoscopys get this product 1.0g, accurately weighed, add appropriate distilled water ultrasonic dissolution, are settled to 100ml, obtain need testing solution with water.Precision pipettes need testing solution 1.0ml, and the method preparation under the preparation of sighting target directrix curve, measures absorbance in accordance with the law, and calculate the content of total peptide in need testing solution with standard curve.
Embodiment three: cockroach urgees the Mouse Acute Toxicity experiment of oral cavity tissue reparation and nutrition gingiva activity extract
The healthy Kunming mouse of cleaning grade, male and female half and half, disposablely to buy the last week in experiment, body weight 25 ~ 30g, 140 mices are divided into 7 groups at random, i.e. blank group, Normal group, medicine group (300mg/Kg, 600mg/Kg, 1200mg/Kg, 2400mg/Kg, 5000mg/Kg), often organizes 20.Blank group, Normal group press weight 0.2ml/10g gavage 0.9% normal saline, medicine components does not give the drug suspension of 300mg/Kg, 600mg/Kg, 1200mg/Kg, 2400mg/Kg, 5000mg/Kg, every day gavage once, interval feed in 2 hours after administration, successive administration 14 days, obtains the median lethal dose(LD 50) LD50 of test mice.
Mice Continuous Observation after gastric infusion has no dead for 14 days, and well-grown, and the mice mental status, behavioral activity, appearance color, diet, defecation are all normal, and body weight all has increase.Put to death the internal organs changes all without exception such as rear the perusal heart, liver, spleen, lung, kidney, and get liver organization and carry out pathological section, Masson dyes, respectively organize the pathological change of hepatic tissue at Electronic Speculum basis of microscopic observation, each group hepatocyte size is even, all without degeneration, necrosis, lobules of liver structure exists, hepatic cords marshalling around central vein, portal area is clear debates, show that extract has no side effect.Test mice LD50 is greater than 5000mg/Kg, and show that test mice is without seriously poisoning danger, extract is nontoxic.Test result is in table 1.
Table 1 cockroach urgees the acute toxicity testing result of oral cavity tissue reparation and nutrition gingiva activity extract
Embodiment four: purify with activated carbon the cockroach extractive obtained and the repair of oral ulcer is tested
1, the preparation of need testing solution: be mixed with 200mg/ml by purifying the cockroach extractive sterilized water for injection obtained with activated carbon, for subsequent use.
2, zoopery: the rabbit of common raising is divided at random treatment group, matched group and blank group, often organizes 8.Use sodium phenobarbital is anaesthetized, get the volumetric flask 1 that bottleneck diameter is 1cm, built-in 30mL volume ratio is the glacial acetic acid of 30%, bottleneck blocks with absorbent cotton, to burn 1 minute by bicker mucosa place after glacial acetic acid moistening absorbent cotton on the right side of rabbit, normal saline flushing 5 minutes, within 24 hours, form ulcer, after forming ulcer, by family's rabbit anesthesia when every day 8:00,12:00,16:00, blank group is not treated, treatment group uses the need testing solution of 1 preparation, matched group uses watermelon crystal spray, and the course for the treatment of is 5 days, measures rabbit ulcer surface maximum gauge every day and record.
3, efficacy determination: ulcer disappears for being cured substantially; Ulcer maximum gauge reduces 1/2 of initial value and is effective above; Ulcer maximum gauge reduces less than 1/2 of initial value for invalid.Pathology: after terminating the course for the treatment of, puts to death rabbit, gets its both sides mucous membrane tissue and carry out pathology paired observation.
4, interpretation of result: when the treatment of group comparitive study was to the 2nd day, treatment group curative effect is better than matched group and blank group; When treatment was to the 5th day, treatment group and matched group Different therapeutical effect significantly, and are all better than blank group (P<0.05), in table 2.
The therapeutic effect of table 2 pair oral ulcer compares (n=8)
Conclusion: animal experimental data shows, treatment group curative effect is obviously better than matched group.
Embodiment five: purify with activated carbon the cockroach extractive obtained and the therapeutical effect of periodontitis is tested
Periodontitis refers to the chronic infectious disease occurring in tooth supporting tissue, the formation of Periodontal Supporting Tissue inflammation, periodontal pocket, Progressive symmetric erythrokeratodermia attachment loss and frontal resorption can be caused, finally causing odontoseisis and pulled out, is the first cause of China adult loss of tooth.
1, the preparation of need testing solution: be mixed with 200mg/ml by purifying the cockroach extractive sterilized water for injection obtained with activated carbon, for subsequent use.
2, zoopery: by cleaning grade male SD rat 24, at 3 monthly ages, body weight 180g ± 20g, is divided into 3 groups by rat by weight average in experiment: Normal group (A): not modeling, not administration (8); Blank group (B): ligation+normal saline gavage group (8); Experimental group (C): ligation+cockroach extractive gavage group (8); To B group and C group rat after 1% sodium pentobarbital (4ml/kg) intraperitoneal injection of anesthesia, the maxillomandibular first molar in random selecting side adopts with 3.0 silk threads in the horizontal ligation of experiment sound of baby talk cervical region gum edge, and along in tip of a root direction press-in gingival sulcus, after ligation, next day starts administration, cockroach extractive is to organize dosage for 100mg/kg, successive administration put to death each group of rat after 6 weeks, drew materials.
3, efficacy determination: rat is separated bilateral mandibular after putting to death respectively, intercept the section of grinding one's teeth in sleep, 4% neutral formalin is fixed, EDTA decalcification, gradient alcohol dehydration, paraffin embedding.Get first, second middle-distant direction section of grinding one's teeth in sleep, conventional H E dyes, om observation.Epithelium rule in A group gingival epithelium and ditch, complete, without periodontal pocket formation, alveolar ridge crest has no obvious absorption, and alveolar ridge crest and furcation area district phatnoma bone height are without significant change.All there is ulcer in B group and C group rat interdental papilla epithelium, downright bad, epithelial peg is uprushed life, but B group inflammatory cell to invade profit more obvious.B group and C group rat alveolar ridge crest all can be observed bone resorption in various degree, height reduction, but B group furcation area district bone resorption amount (179.67+16.12) is obviously more than C group frontal resorption amount (123.12+5.32), higher than A group (119.34+6.24) absorbtivity.
4, interpretation of result: the inflammatory reaction of result of study display cockroach extractive treatment group periodontal tissue is obviously lighter than matched group, and frontal resorption amount is obviously less than matched group, shows the absorption of cockroach extractive Inhibition test periodontitis alveolar bone.
By above-mentioned experiment, can find out that the present invention prepares simple and easy to do for the extraction of cockroach effective site, the cockroach effective site extracting preparation has good effect to multiple oral diseases such as prevention and therapy oral ulcer, gingivitis, periodontitis, gingival atrophyes.For the new drug and daily oral care product of researching and developing safe and effective treatment oral disease lay the foundation.Cockroach aboundresources, realizes artificial breeding at present completely, and national annual output reaches more than 600 ton, aboundresources.
Although give some embodiments of the present invention, it will be understood by those of skill in the art that without departing from the spirit of the invention herein, can change embodiment herein.Above-described embodiment is exemplary, should using embodiment herein as the restriction of interest field of the present invention.
Claims (10)
1. an extraction preparation method for cockroach effective site, is characterized in that, comprise the following steps:
Cockroach adult is prepared extractum;
Described extractum carries out defat, obtains defat extracting solution;
Described defat extracting solution through activated carbon column chromatography, with alcohol water mixed solvent eluting;
Described eluent is evaporated to without alcohol taste, obtains cockroach effective site.
2. the extraction preparation method of cockroach effective site as claimed in claim 1, is characterized in that: described step cockroach adult being prepared extractum specifically comprises;
By boiling hot after death with the hot water of 50-60 DEG C for the described cockroach adult of culturing time more than 5 months, in 60-70 DEG C of oven dry, be crushed to 10-30 order, obtain cockroach adult powder;
Described cockroach adult powder is put in steeping tank, adds the alcohol water mixed solvent that 3-7 times amount volume fraction is 60-95%, stirs evenly, and in described alcohol water mixed solvent, alcohol comprises methanol, ethanol, ethylene glycol or their mixture;
Cockroach adult powder in described steeping tank extracts, and obtains extracting solution; Described extracting method comprises merceration and extracts and reflux, extract; Merceration extracts, and each immersion more than 72 hours, stirred once at interval of 6 ~ 12 hours therebetween, extract 3 times; Reflux, extract, reflux, extract, 2 ~ 5 hours at 70 ~ 80 DEG C, extracts 3 times;
Described extracting solution aperture is 200 ~ 500 object filter-cloth filterings, and collect filtrate, 50-80 DEG C is evaporated to without alcohol taste, obtains extractum.
3. the extraction preparation method of the cockroach effective site as described in any one of claim 1 or 2, it is characterized in that: described extractum carries out defat, obtaining degreasing method described in defat extracting solution step is the 60-75 DEG C of hot water adding described extractum and extractum volume 2-5 times amount in oily-water seperating equipment, slowly stir along a direction, mixing speed 20-40 rev/min, mixing time is 20-30 minute, be heated to 65-75 DEG C simultaneously, be incubated 1 hour, leave standstill cooling 6-12 hour, after oils and fats is separated, releases water layer, filter, collect filtrate, obtain described defat extracting solution.
4. the extraction preparation method of cockroach effective site as claimed in claim 3, it is characterized in that: described defat extracting solution is through activated carbon column chromatography, be wood substance grain shaped activated carbon with active carbon described in alcohol water mixed solvent elution step, consumption is 2-5 times of defat extracting liquid volume, described degreaser upper prop absorption is used the alcohol water mixed solvent eluting of 50-95% after 2-3 hour, collect eluent, filter.
5. the extraction preparation method of cockroach effective site as claimed in claim 4, it is characterized in that: described eluent is evaporated to without alcohol taste, obtaining concentrating under reduced pressure temperature described in cockroach effective site step is 50-80 DEG C.
6. a cockroach effective site, is characterized in that: the content of total peptide in effective site, in bSA, adopts Folin-phenol method to measure, is no less than 100.0mg/g.
7. cockroach effective site is as preparation treatment and the application preventing oral disease medicine, it is characterized in that: described cockroach effective site is cockroach effective site according to claim 6.
8. cockroach effective site as claimed in claim 7 is as preparation treatment and the application preventing oral disease medicine, it is characterized in that: described oral disease comprises oral ulcer, gingivitis, periodontitis and gingival atrophy; Described medicine comprises medicine, drug regimen and nursing materials.
9. the cockroach effective site as described in any one of claim 7 or 8, as the application of preparation treatment and prevention oral disease medicine, is characterized in that: described medicine, drug regimen and nursing materials comprise the tablet, membrane, liniment, ointment, gel, aerosol, spray and the gargarism that add various adjuvants that pharmaceutics allows and make.
10. cockroach effective site described as claimed in claim 9, as preparation treatment and the application preventing oral disease medicine, is characterized in that: described tablet comprises buccal tablet, effervescent tablet and mouth paster.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410524173.3A CN104523755B (en) | 2014-10-08 | 2014-10-08 | A kind of blattaria active component and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410524173.3A CN104523755B (en) | 2014-10-08 | 2014-10-08 | A kind of blattaria active component and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104523755A true CN104523755A (en) | 2015-04-22 |
| CN104523755B CN104523755B (en) | 2019-06-11 |
Family
ID=52839512
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410524173.3A Active CN104523755B (en) | 2014-10-08 | 2014-10-08 | A kind of blattaria active component and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104523755B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370738A (en) * | 2016-08-18 | 2017-02-01 | 四川好医生攀西药业有限责任公司 | Periplaneta americana medicinal material fingerprint quality determination method |
| CN107488529A (en) * | 2017-07-22 | 2017-12-19 | 深圳诺漫斯生物科技有限公司 | The low foam laundry liquid and preparation method of a kind of antipathogenic composition containing American cockroach |
| CN107970199A (en) * | 2018-01-03 | 2018-05-01 | 昆明赛诺制药股份有限公司 | A kind of toothpaste of the extract containing American cockroach |
| CN108014137A (en) * | 2016-11-02 | 2018-05-11 | 浙江京新药业股份有限公司 | American-cockroach-extract lozenge |
| CN110592094A (en) * | 2018-06-13 | 2019-12-20 | 四川好医生攀西药业有限责任公司 | Periplaneta americana fibroblast growth factor and expression and purification method thereof |
| CN113717243A (en) * | 2021-08-10 | 2021-11-30 | 大理大学 | Cockroach glycoprotein and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101254212A (en) * | 2008-03-25 | 2008-09-03 | 浙江大学 | Use of valid target of periplaneta americana |
| CN101957345A (en) * | 2010-03-05 | 2011-01-26 | 浙江京新药业股份有限公司 | Method for detecting active ingredients in American cockroach extract |
| CN102302518A (en) * | 2011-08-12 | 2012-01-04 | 云南省腾冲制药厂 | Cockroach extractive and prepared oral care composition as well as preparation method and applications |
-
2014
- 2014-10-08 CN CN201410524173.3A patent/CN104523755B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101254212A (en) * | 2008-03-25 | 2008-09-03 | 浙江大学 | Use of valid target of periplaneta americana |
| CN101957345A (en) * | 2010-03-05 | 2011-01-26 | 浙江京新药业股份有限公司 | Method for detecting active ingredients in American cockroach extract |
| CN102302518A (en) * | 2011-08-12 | 2012-01-04 | 云南省腾冲制药厂 | Cockroach extractive and prepared oral care composition as well as preparation method and applications |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370738A (en) * | 2016-08-18 | 2017-02-01 | 四川好医生攀西药业有限责任公司 | Periplaneta americana medicinal material fingerprint quality determination method |
| CN108014137A (en) * | 2016-11-02 | 2018-05-11 | 浙江京新药业股份有限公司 | American-cockroach-extract lozenge |
| CN107488529A (en) * | 2017-07-22 | 2017-12-19 | 深圳诺漫斯生物科技有限公司 | The low foam laundry liquid and preparation method of a kind of antipathogenic composition containing American cockroach |
| CN107970199A (en) * | 2018-01-03 | 2018-05-01 | 昆明赛诺制药股份有限公司 | A kind of toothpaste of the extract containing American cockroach |
| CN110592094A (en) * | 2018-06-13 | 2019-12-20 | 四川好医生攀西药业有限责任公司 | Periplaneta americana fibroblast growth factor and expression and purification method thereof |
| CN110592094B (en) * | 2018-06-13 | 2023-04-28 | 四川好医生攀西药业有限责任公司 | American cockroach fibroblast growth factor and expression and purification method thereof |
| CN113717243A (en) * | 2021-08-10 | 2021-11-30 | 大理大学 | Cockroach glycoprotein and preparation method and application thereof |
| CN113717243B (en) * | 2021-08-10 | 2023-06-27 | 大理大学 | Cockroach glycoprotein and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104523755B (en) | 2019-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104523755A (en) | Cockroach effective part and preparation method and application thereof | |
| CN102988828B (en) | Preparation method of dendrobium officinale granula | |
| CN107929420A (en) | Improve energy and blood of human body function and improve the Chinese medicine composition and detection method of immunity | |
| CN105920067A (en) | Sunflower calathide extract containing polysaccharide, flavonoid and alkaloid and preparation method of sunflower calathide extract | |
| CN1331488C (en) | Prunella spike extract and its preparation method and use | |
| CN100389794C (en) | Medicine for treating chronic kidney failure and its preparing process | |
| CN105194421A (en) | Self-heal health-care oral solution capable of reducing blood fat and preparation method thereof | |
| CN101953984B (en) | Medicinal composition for treating urarthritis, preparation method thereof, preparation thereof and use thereof | |
| CN102389496B (en) | Chinese medical composition for treating hepatitis and preparation method thereof | |
| CN110368445A (en) | A kind of Chinese medicine composition and its preparation method and application for treating psoriasis | |
| CN103316166B (en) | Tibetan medicine for treating hemorrhoids and preparation method thereof | |
| CN102793830A (en) | Pronephrium triphyllum-rheum officinale gel and preparation method thereof | |
| CN100363046C (en) | Prepn for treating fatty liver and alcoholic liver and its prepn process | |
| CN103100028A (en) | Soft capsule favorable for reducing alcoholic liver injury and preparation method thereof | |
| CN108452240B (en) | Anti-tumor traditional Chinese medicine composition and application thereof | |
| CN103893101A (en) | Ganoderma lucidum and pollen anti-aging cream | |
| CN1253733A (en) | Formula of liver-protecting milk powder and its production process | |
| CN100493522C (en) | Medicinal composition of oxymatrine and polysaccharide | |
| CN106177082A (en) | A kind of preparation method and application of compound phellodendron bark liquid varnish | |
| CN103263508B (en) | Traditional Chinese medicine composition for treating swine toxoplasmosis as well as preparation and application thereof | |
| CN101053588A (en) | Preparation method for compound arabinogalactan film coated tablets | |
| CN104857113A (en) | Preparation method of anti-radiation traditional Chinese medicine composition | |
| CN104840747B (en) | Chinese medicine composition with antithyroid cancer activity and its preparation method and application | |
| CN104721682B (en) | A kind of compound composite medicament for treating ulcerative colitis and preparation method thereof | |
| CN102416087A (en) | Chinese medicinal composition for treating pelvic inflammatory disease and preparation method as well as application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240125 Address after: No. 8 Zhuhui Road, Shengci Town, Jingjiang City, Taizhou City, Jiangsu Province, 214500 Patentee after: Jingjiang Tianli Feed Technology Co.,Ltd. Country or region after: China Address before: 671003 No.2 Hongsheng Road, Dali ancient city, Dali Bai Autonomous Prefecture, Yunnan Province Patentee before: Dali University Country or region before: China |
|
| TR01 | Transfer of patent right |