CN104857113A - Preparation method of anti-radiation traditional Chinese medicine composition - Google Patents
Preparation method of anti-radiation traditional Chinese medicine composition Download PDFInfo
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- CN104857113A CN104857113A CN201510276896.0A CN201510276896A CN104857113A CN 104857113 A CN104857113 A CN 104857113A CN 201510276896 A CN201510276896 A CN 201510276896A CN 104857113 A CN104857113 A CN 104857113A
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- extract
- radix glycyrrhizae
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- licorice
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Abstract
The invention relates to the field of plant chemistry, in particular to a preparation method of an anti-radiation traditional Chinese medicine composition. The preparation method comprises the following steps: after smashing licorice powder, performing ultrasonic treatment for 60 minutes in a 70% ethyl alcohol solvent to obtain a licorice extract, wherein the ultrasonic power is 80 W, the ultrasonic frequency is 30 kHz, and the material-liquid ratio of the licorice powder and the ethyl alcohol solvent is 1:10 (m/v); performing vacuum concentration on the extract to form an extract, then performing digestion on astragalus membranaceus by using a 70% ethyl alcohol for 24 hours, concentrating to form an astragalus membranaceus extract, performing azeotropic backflow on the licorice extract, the astragalus membranaceus extract, jasmine flowers, roses and white chrysanthemum, and filtering to obtain the anti-radiation traditional Chinese medicine composition. The invention further discloses a licorice extraction technology which is simple in technology, high in product purity and easy for industrial popularization; moreover, the medicine use of a product obtained by utilizing the licorice extraction technology is also provided, and the product is high in market value.
Description
Technical field
The present invention relates to field of phytochemistry, particularly relate to a kind of preparation method of anti-radiation Chinese medicine composition.
Background technology
Radix Glycyrrhizae is the dry rhizome of glycyrrhizic legume, Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L., is clinical conventional herbal variety.
Radix Glycyrrhizae property is sweet, flat, has effect of invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.
Radix Glycyrrhizae contains number of chemical composition, and main component has glycyrrhizic acid, liquiritin etc.The chemical composition of Radix Glycyrrhizae is very complicated; from Radix Glycyrrhizae, isolated compound has the tens of kinds of compounds such as glycyrrhizin, enoxolone, liquiritin, isoliquiritin, neoliquriitin, neoisoliquiritin, glycyrrhizin, isoliquiritigenin and licoricidin (+)-Licoricidin., glycyrol, isoglycyrol, 7-methylcoumarin essence, umbelliferone so far, but these compositions and quantity usually can be different with the difference of the factors such as the kind of Radix Glycyrrhizae, planting area, collecting time.Large quantifier elimination shows, glycyrrhizin and Flavonoid substances are most important biological active substanceies in Radix Glycyrrhizae, are mainly present in the part within licorice epidermis.
Summary of the invention
The invention discloses a kind of Radix Glycyrrhizae extract, described Radix Glycyrrhizae extract is after Radix Glycyrrhizae powder is beaten powder, and in the alcohol solvent of 70%, supersound process obtains for 60 minutes, and ultrasonic power is 80W, and supersonic frequency is 30kHz.
Preferably, the solid-liquid ratio of Radix Glycyrrhizae powder and alcohol solvent is 1:10(m/v).
Simultaneously, the invention also discloses the assay method of glycyrrhizic acid content in Radix Glycyrrhizae extract, described assay method detects its absworption peak at 252 wavelength places with ultraviolet spectrophotometry, and by glycyrrhizic acid standard substance production standard curve, thus obtain the content of glycyrrhizic acid in Radix Glycyrrhizae extract.
Further, we also disclosed the described application of glycyrrhizic acid extract in anti-radiation Chinese medicine composition, described anti-radiation Chinese medicine composition also comprise respectively with the Radix Astragali of the quality such as Radix Glycyrrhizae extract, Flos Jasmini Sambac, Flos Rosae Rugosae, Flos Chrysanthemi.
Meanwhile, we also disclosed the application of described glycyrrhizic acid extract in preparation treatment gingivitis medicine.
Further, when preparing treatment gingivitis medicine, also comprise and the fresh Herba Plantaginis of the quality such as Radix Glycyrrhizae extract and fresh Herba Menthae.
Further, when preparing treatment gingivitis medicine, also include the shagreen Ovum Anas domestica with the quality such as Radix Glycyrrhizae extract.
A preparation method for anti-radiation Chinese medicine composition, comprises the steps:
According to aforesaid method by ultrasonic extraction Radix Glycyrrhizae, obtain Radix Glycyrrhizae extract, extract concentrating under reduced pressure is formed extractum, then by the Radix Astragali with 70% alcohol steep after 24 hours, concentrated formation Radix Astragali extractum, by Radix Glycyrrhizae extractum, Radix Astragali extractum, Flos Jasmini Sambac, Flos Rosae Rugosae, the backflow of Flos Chrysanthemi azeotropic, filter, obtain anti-radiation Chinese medicine composition.
Treat a preparation method for gingivitis medicine, comprise the steps:
According to aforesaid method by ultrasonic extraction Radix Glycyrrhizae, obtain Radix Glycyrrhizae extract, extract concentrating under reduced pressure is formed extractum, then Radix Glycyrrhizae extractum and fresh Herba Plantaginis azeotropic is refluxed, filter, get filtrate, fresh Herba Menthae adds in filtrate, distillation, collects distillate, is treatment gingivitis medicine.
The present invention does not only disclose that a kind of technique is simple, product purity is high, be easy to the extraction process of Glycyrrhiza uralensis of industrialization promotion, gives the medicinal usage of this technique products obtained therefrom simultaneously, has good market value.
Detailed description of the invention
Embodiment 1 tests the assay with Radix Glycyrrhizae acid
Precision takes glycyrrhizic acid standard substance 0.0230g, and dissolve with 50% ethanol water, obtained concentration is the standard solution of 0.23mg/mL.
Respectively precision measure 0.2,0.4,0.6,0.8,1.0ml standard solution, be diluted to 10mL with 50% ethanol water, detect it at 252nm place absorbance, obtain standard curve:
。
The investigation of embodiment 2 ultrasonic extraction condition
The investigation of 2.1 ultrasonic powers
Radix Glycyrrhizae powder and ethanol soak with the ratio of 1g:10ml, select different ultrasonic powers to extract 60 minutes respectively, by the solution sucking filtration after process.
Obtain the glycyrrhizic acid content in Radix Glycyrrhizae extract according to the method in embodiment 1, utilize the glycyrrhizic acid in Radix Glycyrrhizae extract and the glycyrrhizic acid ratio in Radix Glycyrrhizae, obtain extraction ratio, the relation of extraction ratio and ultrasonic power is shown in Fig. 1.
The condition of 2.2 ultrasonic times is investigated
Radix Glycyrrhizae powder crosses 20 mesh sieves, and ultrasonic power is 80W, and solid-liquid ratio is 1:10, and be dipped to after medical material all soaks, supersound process 30 minutes, 40 minutes, 50 minutes, 60 minutes, 70 minutes, 80 minutes respectively, by the solution sucking filtration after supersound process.
Obtain the glycyrrhizic acid content in Radix Glycyrrhizae extract according to the method in embodiment 1, utilize the glycyrrhizic acid in Radix Glycyrrhizae extract and the glycyrrhizic acid ratio in Radix Glycyrrhizae, obtain extraction ratio, the relation of extraction ratio and time is shown in Fig. 2.
The investigation of 2.3 ultrasonic Treatment solvents
Radix Glycyrrhizae powder crosses 20 mesh sieves, and ultrasonic power is 80W, and solid-liquid ratio is 1:10, after all soaking respectively, continues supersound process further, by the solution sucking filtration after supersound process with alcohol solution dipping to the medical material of variable concentrations.
Obtain the glycyrrhizic acid content in Radix Glycyrrhizae extract according to the method in embodiment 1, utilize the glycyrrhizic acid in Radix Glycyrrhizae extract and the glycyrrhizic acid ratio in Radix Glycyrrhizae, obtain extraction ratio, the relation of extraction ratio and solvent strength is shown in Fig. 3.
The orthogonal experiment of 2.4 ultrasonic Treatment integrated conditions
Influence factor and level are in table 1:
Orthogonal experiment results is in table 2:
。
Therefore, determine that optimum extraction process is: described Radix Glycyrrhizae extract is after Radix Glycyrrhizae powder is beaten powder, and supersound process obtains for 60 minutes in the alcohol solvent of 70%, and ultrasonic power is 80W, and supersonic frequency is 30kHz.
Preferably, the solid-liquid ratio of Radix Glycyrrhizae powder and alcohol solvent is 1:10(m/v).
Embodiment 3
After Radix Glycyrrhizae powder is beaten powder, cross 20 mesh sieves, in the alcohol solvent of 70%, supersound process obtains for 60 minutes, and ultrasonic power is 80W, and supersonic frequency is 30kHz, and the solid-liquid ratio of Radix Glycyrrhizae powder and alcohol solvent is 1:10(m/v).Obtained Radix Glycyrrhizae extract.
Embodiment 4
Ultrasonic extraction Radix Glycyrrhizae is passed through according to the method for embodiment 3, obtain Radix Glycyrrhizae extract, extract concentrating under reduced pressure is formed extractum, then by the Radix Astragali with 70% alcohol steep after 24 hours, concentrated formation Radix Astragali extractum, by Radix Glycyrrhizae extractum, Radix Astragali extractum, Flos Jasmini Sambac, Flos Rosae Rugosae, the backflow of Flos Chrysanthemi azeotropic, filter, obtain anti-radiation Chinese medicine composition.
Embodiment 5
Select the male C57 mice of body weight 18-22g 40.Mouse feeder ambient temperature 21 DEG C ± 2 DEG C, phase
Humidity 50% ± 10%, 12 hours light 12 h dark, precuring 3 days, is divided into 3 groups, i.e. normal group at random, radiation group, experimental group (selecting the Chinese medicine composition of gained in embodiment 4), often organizes 10.The disposable whole body uniform irradiation of 60Co gamma-rays, exposure dose is 7.5Gy, and close rate is 213.16cGy/min, according to after gastric infusion immediately, normal group and irradiation group give equal-volume distilled water, Chinese medicine composition group 50mg/kg, successive administration 5 days, Continuous Observation mice 30 days.
Result: the Chinese medicine composition containing Radix Glycyrrhizae extract improves mouse survival rate 30%, extends mean survival time, and difference is extremely remarkable, and survival mice is in good condition.
Embodiment 5
Pass through ultrasonic extraction Radix Glycyrrhizae according to the method for embodiment 3, obtain Radix Glycyrrhizae extract, extract concentrating under reduced pressure is formed extractum, then Radix Glycyrrhizae extractum and fresh Herba Plantaginis azeotropic is refluxed, filter, get filtrate, fresh Herba Menthae adds in filtrate, distillation, collects distillate, is treatment gingivitis medicine.
Embodiment 6
Under narcotism, select bacterial plaque scoring 2-4(bacterial plaque area dress computer more than 25% ~ 75% buccal side dental surface), gingivitis scoring at the slight or medium gingivitis of 1-3() beasle dog 12, be divided into experimental group and matched group at random, often organize 6.After Animal Anesthesia, to gingivitis and the calculus scores at its third molar place, with after dental plaque fluid colour developing, bacterial plaque is marked.
The gingival tissues simultaneously cutting 3mm in the vestibule region of the 3rd premolar teeth of its upper left side carries out HE dyeing, marks under light microscopic to inflammatory infiltrate.
The grading system of gingivitis is:
0: there is no gingivitis
1: very minimal gingival inflammation (red and swollen, to touch not hemorrhage),
2: minimal gingival inflammation (red and swollen, to touch delayed bleeding),
3: medium gingivitis (red and swollen, hemorrhage immediately after touching),
4: serious gum inflammation (ulcer, hematostaxis).
The grading system of dental calculus accounts for the area classification of buccal side dental surface according to dental calculus,
0:0%
1：＜25%，
2:25~49%，
3:50~74%，
4：＞75%。
Inflammatory infiltrate is marked:
0: what have the single or rickle of the chronic inflammation cell be dispersed in is arranged in surface epithelial cell,
1: have on epithelial side sparse but connect into band inflammatory cell assemble,
2: around epithelial cell, the inflammatory cell of a large amount of infiltration instead of normal gingival fiber,
3: extensive in the tissue, a large amount of inflammatory cell assembled instead of most gingival fiber.
Then respectively with infiltrating the tooth and the gums that have the cotton balls for the treatment of gingivitis medicine in embodiment 5 to cover animal lip side and area ace, normal saline contrasts, each 10 seconds, twice daily, continues 14 days.Within 15th day, again by Animal Anesthesia, and to its bacterial plaque, gingivitis and calculus scores, the gingival tissues simultaneously cutting 3mm in the vestibule region of the 3rd premolar teeth of its upper right side carries out HE dyeing, marks under light microscopic to inflammatory infiltrate.
Result shows that bacterial plaque and gingivitis symptom score significantly reduce at experimental group, p < 0.05, and matched group is without significant difference, and p > 0.1, concrete outcome is in table 3 after treatment.
Table 3:
Claims (1)
1. a preparation method for anti-radiation Chinese medicine composition, comprises the steps:
(1) after Radix Glycyrrhizae powder being beaten powder, in the alcohol solvent of 70%, supersound process obtains for 60 minutes, and ultrasonic power is 80W, and supersonic frequency is 30kHz, and the solid-liquid ratio of Radix Glycyrrhizae powder and alcohol solvent is 1:10(m/v), obtain Radix Glycyrrhizae extract;
(2) extract concentrating under reduced pressure is formed extractum, then the Radix Astragali after 24 hours with 70% alcohol steep, is concentrated and forms Radix Astragali extractum, by Radix Glycyrrhizae extractum, Radix Astragali extractum, Flos Jasmini Sambac, Flos Rosae Rugosae, the backflow of Flos Chrysanthemi azeotropic, filter, obtain anti-radiation Chinese medicine composition.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106110292A (en) * | 2016-08-22 | 2016-11-16 | 刘传文 | A kind of Chinese and western medicinal composition for Ultrasonography radioprotective and preparation method |
| CN107213277A (en) * | 2017-06-27 | 2017-09-29 | 广州中医药大学 | A kind of pharmaceutical composition and preparation method thereof, application |
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| CN102206242A (en) * | 2011-03-28 | 2011-10-05 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Application of beta-glycyrrhizic acid and derivatives thereof to radiation protection |
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- 2015-05-27 CN CN201510276896.0A patent/CN104857113A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102206242A (en) * | 2011-03-28 | 2011-10-05 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Application of beta-glycyrrhizic acid and derivatives thereof to radiation protection |
Non-Patent Citations (2)
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| 张海燕等: "超声波法提取甘草中的甘草酸", 《河南科学》 * |
| 李宗山等: "黄芪抗γ射线辐射作用的研究", 《时珍国医国药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106110292A (en) * | 2016-08-22 | 2016-11-16 | 刘传文 | A kind of Chinese and western medicinal composition for Ultrasonography radioprotective and preparation method |
| CN107213277A (en) * | 2017-06-27 | 2017-09-29 | 广州中医药大学 | A kind of pharmaceutical composition and preparation method thereof, application |
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