CN104491190A - Preparation method of Chinese eaglewood leaf extract - Google Patents
Preparation method of Chinese eaglewood leaf extract Download PDFInfo
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- CN104491190A CN104491190A CN201410819997.3A CN201410819997A CN104491190A CN 104491190 A CN104491190 A CN 104491190A CN 201410819997 A CN201410819997 A CN 201410819997A CN 104491190 A CN104491190 A CN 104491190A
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- lignum aquilariae
- aquilariae resinatum
- resinatum leaf
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- leaf
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/83—Thymelaeaceae (Mezereum family), e.g. leatherwood or false ohelo
- A61K36/835—Aquilaria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of a Chinese eaglewood leaf extract. The preparation method comprises the following steps: firstly carrying out ultrasonic treatment on Chinese eaglewood leaves, carrying out pressurization extrusion until the Chinese eaglewood leaves are crushed to obtain Chinese eaglewood leaf powder, and carrying out six-stage countercurrent extraction by an ultrasonic treatment liquid under the condition that a solid-to-liquid ratio is (1:4)-(1:5), single stage extraction time is 30-60min and extraction temperature is 50-55 DEG C. According to the preparation method of the Chinese eaglewood leaf extract, with combination of ultrasonic treatment and a multi-stage countercurrent extraction technology, the obtained extract is high in content of the effective component, high in extraction ratio of the raw material, simple in method and easy in operation.
Description
Technical field
The present invention relates to a kind of extracting method of effective ingredients in plant, specifically a kind of preparation method of Lignum Aquilariae Resinatum leaf extract.
Background technology
Lignum Aquilariae Resinatum is that thymelaeceae (Thymelaeaceae) Lignum Aquilariae Resinatum belongs to (Aquilaria) Lignum Aquilariae Resinatum (Aquilaria sinensis (Lour.) Gilg) and congener Lignum Aquilariae Resinatum (Aquilria agallocha Roxb.) resiniferous timber.The biological element amount that Lignum Aquilariae Resinatum leaf contains needed by human is extremely abundant, as calcium, zinc, ferrum, manganese etc. and vitamin, be the supplement of the good particular nutrient of human body, and multiple disadvantage factor in human body can be eliminated that there is QI invigorating benefit essence, adjusting blood lipid, dredging internal organs function, relieving mental strain and helping sleep, blood fat reducing, help sleep, effect of skin care.
Prior art has disclosed and has utilized the application of Lignum Aquilariae Resinatum leaf in the various health food of preparation, tea, medicine, medicated cigarette, health promoting pillow are first-class.Such as, CN 102613356B disclose a kind of via wilting, with glycyrrhizic aqueous solution moistening, ferment in second time, cure the preparation method obtaining Lignum Aquilariae Resinatum leaf tea; It is containing Lignum Aquilariae Resinatum Lao Ye 30-40% that CN 101438827B discloses a kind of health food, Lignum Aquilariae Resinatum tender leaf 15-17.5%, Radix Morindae Officinalis 15-17.5%, Herba Cistanches 15-17.5%, Radix Panacis Quinquefolii 15-17.5%; CN102524950B discloses one and utilizes Lignum Aquilariae Resinatum leaf, QINGZHU leaf and normally used cigarette shreds to account for full cigarette 40% by tobacco shred, Lignum Aquilariae Resinatum leaf accounts for full cigarette 40%, QINGZHU leaf accounts for full cigarette 19%, and the ratio that cut-tobacco reinforcing alcoholized biological preparation accounts for 1% is mixed with the health cigarette obtained.
The current mainly solvent extraction method of extracting method of Lignum Aquilariae Resinatum leaf effective ingredient.CN101011499B discloses a kind of method of Lignum Aquilariae Resinatum leaf extract, is by the leaf of Lignum Aquilariae Resinatum percolation or reflux, extract, after soaking, reconcentration and get final product, and is wherein the ethanol of 10 ~ 80% for the solvent of percolation or reflux, extract, is weight percentage.The traditional batch technique that solvent method extracts after continuing to use for many years always and pulverizing, the object that raw material is first pulverized is to accelerate extraction rate, but also brings the problems such as reinforced discharging labor intensity is large, solid-liquor separation is difficult, solvent consumption is large, energy consumption is large, efficiency is low, production is unstable simultaneously.
Summary of the invention
The object of the invention is to provide the preparation method of the Lignum Aquilariae Resinatum leaf extract that a kind of rational technology, active constituent content are high, extraction efficiency is high, simple to operate.
For solving the problem, the technical solution adopted in the present invention is: a kind of preparation method of Lignum Aquilariae Resinatum leaf extract is following concrete steps:
(1) select the fresh leaf of fresh, dry Lignum Aquilariae Resinatum leaf, clean, remove petiole, being placed on mass fraction is in the alcoholic solution of 75 ~ 90%, supersound process 3 ~ 10min under frequency 40 ~ 50kHz, ultrasonic end, retains sonification fluid, for subsequent use;
(2) take out the Lignum Aquilariae Resinatum leaf after supersound process, 40 ~ 50 DEG C of oven dry, then carry out pressurization expanded to pulverizing, crossing 80 mesh sieves, obtain Lignum Aquilariae Resinatum leaf powder;
(3) Lignum Aquilariae Resinatum leaf powder is adopted continous way extractor, 6 stage countercurrent extractions are carried out with the sonification fluid continuous circulation of step (1) spray, extractant condition is: Lignum Aquilariae Resinatum leaf powder and sonification fluid solid-to-liquid ratio are 1:4 ~ 1:5, and the single-stage extraction time is 30 ~ 60min, extraction temperature 50 ~ 55 DEG C;
(4) extraction terminates, and extract is reclaimed the ethanol in extract through distilling under reduced pressure, obtain extractum, extractum being concentrated into Baume degrees is 20-25 degree simultaneously, then at 55 ~ 60 DEG C of vacuum dryings, and be crushed to 100 orders, obtain Lignum Aquilariae Resinatum leaf extract.
Preferably, during described supersound process, the solid-to-liquid ratio of the ethanol of Lignum Aquilariae Resinatum leaf and mass fraction 75 ~ 90% is 1:4 ~ 1:5.
Preferably, described supersound process condition is as follows: supersound process 7min under frequency 45kHz.
Preferably, described extractant condition is: the solid-to-liquid ratio of the ethanol of Lignum Aquilariae Resinatum leaf and mass fraction 75 ~ 90% is 1:5, and the single-stage extraction time is 40min, extraction temperature 50 DEG C.
Lignum Aquilariae Resinatum leaf of the present invention is the leaf that thymelaeceae (Thymelaeaceae) Lignum Aquilariae Resinatum genus (Aquilaria) Lignum Aquilariae Resinatum (Aquilaria sinensis (Lour.) Gilg) and/or congener Lignum Aquilariae Resinatum (Aquilria agallocha Roxb.) are set.
Adopt the inventive method to extract the Lignum Aquilariae Resinatum leaf extract obtained and can be used for preparation blood fat reducing, the medicine of loosening bowel to relieve constipation or the health product medically approved.
The present invention relative to the beneficial effect of prior art is: the preparation method of Lignum Aquilariae Resinatum leaf extract of the present invention, supersound process is adopted to combine with multi-stage counter current extraction technology, the extract active constituent content obtained is high, and raw material abstraction rate is high, and method is simple to operation.
Detailed description of the invention
Be described in further details the present invention below by embodiment, these embodiments are only used for the present invention is described, do not limit the scope of the invention.
Embodiment 1: adopt following method to prepare Lignum Aquilariae Resinatum leaf extract of the present invention:
(1) select the fresh leaf of fresh, dry Lignum Aquilariae Resinatum leaf, clean, remove petiole, being placed on mass fraction is in the alcoholic solution of 75%, supersound process 7min under frequency 45kHz, ultrasonic end, retains sonification fluid, for subsequent use; The solid-to-liquid ratio of the ethanol of Lignum Aquilariae Resinatum leaf and mass fraction 75% is 1:5;
(2) take out the Lignum Aquilariae Resinatum leaf after supersound process, 45 DEG C of oven dry, then carry out pressurization expanded to pulverizing, crossing 80 mesh sieves, obtain Lignum Aquilariae Resinatum leaf powder;
(3) Lignum Aquilariae Resinatum leaf powder is adopted continous way extractor, 6 stage countercurrent extractions are carried out with the sonification fluid continuous circulation of step (1) spray, extractant condition is: Lignum Aquilariae Resinatum leaf powder and sonification fluid solid-to-liquid ratio are 1:5, and the single-stage extraction time is 40min, extraction temperature 50 DEG C;
(4) extraction terminates, and extract is reclaimed the ethanol in extract through distilling under reduced pressure, obtain extractum, extractum being concentrated into Baume degrees is 25 degree simultaneously, then at 60 DEG C of vacuum dryings, and be crushed to 100 orders, obtain Lignum Aquilariae Resinatum leaf extract.
Embodiment 2: adopt following method to prepare Lignum Aquilariae Resinatum leaf extract of the present invention:
(1) select the fresh leaf of fresh, dry Lignum Aquilariae Resinatum leaf, clean, remove petiole, being placed on mass fraction is in the alcoholic solution of 80%, supersound process 5min under frequency 40kHz, ultrasonic end, retains sonification fluid, for subsequent use; The solid-to-liquid ratio of Lignum Aquilariae Resinatum leaf and ethanol is 1:4;
(2) take out the Lignum Aquilariae Resinatum leaf after supersound process, 40 DEG C of oven dry, then carry out pressurization expanded to pulverizing, crossing 80 mesh sieves, obtain Lignum Aquilariae Resinatum leaf powder;
(3) Lignum Aquilariae Resinatum leaf powder is adopted continous way extractor, 6 stage countercurrent extractions are carried out with the sonification fluid continuous circulation of step (1) spray, extractant condition is: Lignum Aquilariae Resinatum leaf powder and sonification fluid solid-to-liquid ratio are 1:5, and the single-stage extraction time is 30min, extraction temperature 55 DEG C;
(4) extraction terminates, and extract is reclaimed the ethanol in extract through distilling under reduced pressure, obtain extractum, extractum being concentrated into Baume degrees is 20 degree simultaneously, then at 55 DEG C of vacuum dryings, and be crushed to 100 orders, obtain Lignum Aquilariae Resinatum leaf extract.
Embodiment 3: adopt following method to prepare Lignum Aquilariae Resinatum leaf extract of the present invention:
(1) select the fresh leaf of fresh, dry Lignum Aquilariae Resinatum leaf, clean, remove petiole, being placed on mass fraction is in the alcoholic solution of 90%, supersound process 10min under frequency 40kHz, ultrasonic end, retains sonification fluid, for subsequent use; The solid-to-liquid ratio of the ethanol of Lignum Aquilariae Resinatum leaf and mass fraction 90% is 1:4.5;
(2) take out the Lignum Aquilariae Resinatum leaf after supersound process, 45 DEG C of oven dry, then carry out pressurization expanded to pulverizing, crossing 80 mesh sieves, obtain Lignum Aquilariae Resinatum leaf powder;
(3) Lignum Aquilariae Resinatum leaf powder is adopted continous way extractor, 6 stage countercurrent extractions are carried out with the sonification fluid continuous circulation of step (1) spray, extractant condition is: Lignum Aquilariae Resinatum leaf powder and sonification fluid solid-to-liquid ratio are 1:5, and the single-stage extraction time is 60min, extraction temperature 50 DEG C;
(4) extraction terminates, and extract is reclaimed the ethanol in extract through distilling under reduced pressure, obtain extractum, extractum being concentrated into Baume degrees is 25 degree simultaneously, then at 60 DEG C of vacuum dryings, and be crushed to 100 orders, obtain Lignum Aquilariae Resinatum leaf extract.
Claims (6)
1. a preparation method for Lignum Aquilariae Resinatum leaf extract, is characterized in that: the method comprises following concrete steps:
(1) select the fresh leaf of fresh, dry Lignum Aquilariae Resinatum leaf, clean, remove petiole, being placed on mass fraction is in the alcoholic solution of 75 ~ 90%, supersound process 3 ~ 10min under frequency 40 ~ 50kHz, ultrasonic end, retains sonification fluid, for subsequent use;
(2) take out the Lignum Aquilariae Resinatum leaf after supersound process, 40 ~ 50 DEG C of oven dry, then carry out pressurization expanded to pulverizing, crossing 80 mesh sieves, obtain Lignum Aquilariae Resinatum leaf powder;
(3) Lignum Aquilariae Resinatum leaf powder is adopted continous way extractor, 6 stage countercurrent extractions are carried out with the sonification fluid continuous circulation of step (1) spray, extractant condition is: Lignum Aquilariae Resinatum leaf powder and sonification fluid solid-to-liquid ratio are 1:4 ~ 1:5, and the single-stage extraction time is 30 ~ 60min, extraction temperature 50 ~ 55 DEG C;
(4) extraction terminates, and extract is reclaimed the ethanol in extract through distilling under reduced pressure, obtain extractum, extractum being concentrated into Baume degrees is 20-25 degree simultaneously, then at 55 ~ 60 DEG C of vacuum dryings, and be crushed to 100 orders, obtain Lignum Aquilariae Resinatum leaf extract.
2. the preparation method of a kind of Lignum Aquilariae Resinatum leaf extract according to claim 1, is characterized in that: described supersound process condition is as follows: supersound process 7min under frequency 45kHz.
3. the preparation method of a kind of Lignum Aquilariae Resinatum leaf extract according to claim 1, is characterized in that: during described supersound process, and the solid-to-liquid ratio of the ethanol of Lignum Aquilariae Resinatum leaf and mass fraction 75 ~ 90% is 1:4 ~ 1:5.
4. the preparation method of a kind of Lignum Aquilariae Resinatum leaf extract according to claim 1, is characterized in that: described extractant condition is: the solid-to-liquid ratio of the ethanol of Lignum Aquilariae Resinatum leaf and mass fraction 75 ~ 90% is 1:5, and the single-stage extraction time is 40min, extraction temperature 50 DEG C.
5. the preparation method of a kind of Lignum Aquilariae Resinatum leaf extract according to claim 1, is characterized in that: described Lignum Aquilariae Resinatum leaf is the leaf that thymelaeceae (Thymelaeaceae) Lignum Aquilariae Resinatum genus (Aquilaria) Lignum Aquilariae Resinatum (Aquilaria sinensis (Lour.) Gilg) and/or congener Lignum Aquilariae Resinatum (Aquilria agallocha Roxb.) are set.
6. the preparation method of a kind of Lignum Aquilariae Resinatum leaf extract according to claim 1, is characterized in that: described Lignum Aquilariae Resinatum leaf extract can be used for preparing blood fat reducing, the medicine of loosening bowel to relieve constipation or the health product medically approved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410819997.3A CN104491190A (en) | 2014-12-25 | 2014-12-25 | Preparation method of Chinese eaglewood leaf extract |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410819997.3A CN104491190A (en) | 2014-12-25 | 2014-12-25 | Preparation method of Chinese eaglewood leaf extract |
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| CN104491190A true CN104491190A (en) | 2015-04-08 |
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| CN201410819997.3A Pending CN104491190A (en) | 2014-12-25 | 2014-12-25 | Preparation method of Chinese eaglewood leaf extract |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036153A (en) * | 2016-06-03 | 2016-10-26 | 广西农业职业技术学院 | Chicken feed added with agalloch eaglewood leaf meal |
| CN106509801A (en) * | 2016-10-24 | 2017-03-22 | 汕头市乐美沉香生物科技有限公司 | Making method of extract base powder, and method for making novel agalloch eaglewood vegetal condiment from extract base powder |
| CN115463181A (en) * | 2021-06-10 | 2022-12-13 | 长沙学院 | Preparation method of agilawood leaf extract |
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| JPS6438014A (en) * | 1987-07-31 | 1989-02-08 | Pola Chem Ind Inc | Promoter for hair restoration and hair growth |
| CN101011499A (en) * | 2007-02-05 | 2007-08-08 | 中国药科大学 | Linaloe leaf extract, pharmaceutical and health care uses thereof |
| CN101991715A (en) * | 2010-10-28 | 2011-03-30 | 广东省微生物研究所 | Aquilaria sinensis fruit skin extract and preparation method and application thereof |
-
2014
- 2014-12-25 CN CN201410819997.3A patent/CN104491190A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6438014A (en) * | 1987-07-31 | 1989-02-08 | Pola Chem Ind Inc | Promoter for hair restoration and hair growth |
| CN101011499A (en) * | 2007-02-05 | 2007-08-08 | 中国药科大学 | Linaloe leaf extract, pharmaceutical and health care uses thereof |
| CN101991715A (en) * | 2010-10-28 | 2011-03-30 | 广东省微生物研究所 | Aquilaria sinensis fruit skin extract and preparation method and application thereof |
Non-Patent Citations (3)
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|---|
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| 邓启华等: "超声波连续逆流提取青蒿素的新工艺", 《四川化工》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036153A (en) * | 2016-06-03 | 2016-10-26 | 广西农业职业技术学院 | Chicken feed added with agalloch eaglewood leaf meal |
| CN106509801A (en) * | 2016-10-24 | 2017-03-22 | 汕头市乐美沉香生物科技有限公司 | Making method of extract base powder, and method for making novel agalloch eaglewood vegetal condiment from extract base powder |
| CN115463181A (en) * | 2021-06-10 | 2022-12-13 | 长沙学院 | Preparation method of agilawood leaf extract |
| CN115463181B (en) * | 2021-06-10 | 2024-01-30 | 长沙学院 | Preparation method of agilawood leaf extract |
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