CN104489433A - Preparation process of tortoise shell and poria cocos jelly rich in theanine - Google Patents

Preparation process of tortoise shell and poria cocos jelly rich in theanine Download PDF

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Publication number
CN104489433A
CN104489433A CN201410739803.9A CN201410739803A CN104489433A CN 104489433 A CN104489433 A CN 104489433A CN 201410739803 A CN201410739803 A CN 201410739803A CN 104489433 A CN104489433 A CN 104489433A
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CN
China
Prior art keywords
green tea
preparation technology
theanine
technology according
enzymolysis
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CN201410739803.9A
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Chinese (zh)
Inventor
陆伟
王志鹏
李美红
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JIANGMEN CITY SHENG HE TANG FOODS Co Ltd
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JIANGMEN CITY SHENG HE TANG FOODS Co Ltd
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Priority to CN201410739803.9A priority Critical patent/CN104489433A/en
Publication of CN104489433A publication Critical patent/CN104489433A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/10Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/175Amino acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a preparation process of a tortoise shell and poria cocos jelly rich in theanine. A variety of effective components such as theanine, tea polyphenols and multivitamins are extracted from green tea leaves by adopting an enzyme hydrolysis technology and added into the formula for the traditional tortoise shell and poria cocos jelly by adopting a modern technology, thus the prepared tortoise shell and poria cocos jelly rich in theanine has new efficacies of regulating brain nerve substance delivery, improving menstrual syndromes, alleviating emotional stress and improving sleep quality in addition to the traditional efficacy of clearing away heat, and the traditional medicinal diet can meet the modern needs of people.

Description

A kind of preparation technology being rich in the Guiling Jelly of theanine
Technical field
The invention belongs to field of functional food, particularly a kind of preparation technology being rich in the Guiling Jelly of theanine.
Background technology
Guiling Jelly is a kind of time-honored traditional herbal cuisine, mainly with famous and precious tortoise plastron and smilax for raw material, then be equipped with that the medicines such as the dried rhizome of rehmannia are refining to be formed.Guiling Jelly is warm in nature cool, not cool not dry, all-ages, therefore enjoy people to like, become one of daily edible health products, but the Guiling Jelly product function of present is comparatively single, generally just have clearing heat and detoxicating, remove effect of fire, and there is no other edible effect.
Theanine (L-Theanine) is the free especially propylhomoserin of one in tealeaves there being content; in newly picked and processed tea leaves, content is about about 1%-2%, confirmed in up-to-date medical research theanine have hypotensive, improve memory capability, regulate and neurally in brain pass on the change of material, calmness, improve and comprehensively demonstrate,prove menstrual period and the effect of neuroprotective cell.
Modern accelerates due to rhythm of life, and pressure increase is nervous, is often in sub-health state, as can be seen here, if theanine composition can be introduced traditional Guiling Jelly formula, then can better coordinates modern human needs, play better health-care effect.
But how to introduce and just can reach formula rationally, and be a problem by performance that is maximum for effect of the compositions such as theanine.
Summary of the invention
The technical problem to be solved in the present invention is: provide a kind of preparation technology being rich in the Guiling Jelly of theanine.
The solution that the present invention solves its technical problem is: a kind of preparation technology being rich in the Guiling Jelly of theanine, comprises the following steps:
1) tortoise plastron cleaned, after fragmentation, drop in water and soak 2 ~ 4 hours;
2) add protease by the amount of 5 ~ 100,000 U protease/1g tortoise plastrons, stir and evenly mix, enzymolysis is after 1 ~ 4 hour, and heating makes protease deactivation, then filters while hot, obtains enzymolysis tortoise plastron collagen peptide extract;
3) green tea is dropped in the hot water of 85 DEG C and soak 30 minutes;
4) add cellulase, enzymolysis 2-4 hour by the amount of 4-10 ten thousand U cellulase/1g green tea, heating makes cellulase deactivation, obtains the green tea extractive liquor after enzymolysis;
5) take celestial grass, Poria cocos, honeysuckle, dandelion, Radix Glycyrrhizae and dried rhizome of rehmannia Six-element raw material, drop in water, decoct 1-2 time, each decoction 1-2 hour; Merge decoction liquor, centrifugal filtration, draws decoction extract;
6) merge enzymolysis tortoise plastron protein peptides extract and decoct extract, adding theanine, flavouring, stabilizing agent and green tea extractive liquor after concentrated, after heating is stirred to gelatinization, packing, sterilizing, draw Guiling Jelly finished product.
Preferably, in step 1), tortoise plastron is cleaned and is crushed to fineness and is less than or equal to 2 × 2mm, the consumption of water be the 5-10 of tortoise plastron weight doubly.
Preferably, step 2) described in protease be papain and bromelain.Further preferably, the amount ratio of described papain and bromelain is 1:(1-5).
Preferably, in step 3), described green tea is green tea or green tea powder, and wherein the fineness of green tea is 2 × 2mm; The consumption of water is 8-10 times of green tea weight; The consumption of water is 8-10 times of green tea weight.
Preferably, described cellulase is zytase.Further preferably, when adopting zytase enzymolysis, pH value is 4.0 ~ 6.0, and temperature is 40 ~ 55 DEG C, uses citric acid adjusted to ph.
Preferably, in step 5), the consumption of water is 8 ~ 10 times of Six-element raw material weight.
Preferably, in above-mentioned preparation technology, described Guiling Jelly is prepared from by the raw material of following weight portion, tortoise plastron 5-15 part, green tea 20-30 part, theanine 5-10 part, celestial careless 90-98 part, honeysuckle 12-16 part, Fu Ling 40-60 part, dandelion 12-16 part, dried rhizome of rehmannia 10-15 part, Radix Glycyrrhizae 8-15 part; Described flavouring is rock sugar, and described stabilizing agent is carragheen and/or starch.Further preferably, the ratio of described starch and carragheen is 20-24:1.
The invention has the beneficial effects as follows: preparation technology of the present invention passes through zymolysis technique, extract the plurality of active ingredients (as theanine, Tea Polyphenols and multivitamin material etc.) in green tea, pass through modern technologies again, add in traditional Guiling Jelly formula, the Guiling Jelly obtained both had had traditional effect of clearing heat and removing internal heat, can play again and regulate brain neuroblastoma material Transfer, effect that improve premenstrual syndrome, that extenuate emotional stress and improving water flood quality, give Guiling Jelly with new effect, better make traditional herbal cuisine adapt to modern needs.
Detailed description of the invention
Clear, complete description is carried out, to understand object of the present invention, characteristic sum effect fully below with reference to the technique effect of embodiment to design of the present invention and generation.
Embodiment 1
1) 0.5kg tortoise plastron is cleaned, be crushed to fineness and be less than 2 × 2mm, then drop in the water of 2.5L and soak 2 hours;
2) add by the amount of 80,000 U protease/1g tortoise plastrons the compound protease be made up of papain and bromelain, wherein papain (800,000 U/g) 0.025kg and bromelain (800,000 U/g) 0.025kg, limit edged stirs and evenly mixs; Adjust pH to 6.5, be warming up to 45 DEG C, be incubated after 4 hours, be heated to 100 DEG C and make enzyme-deactivating, then filter while hot, obtain filtrate, be i.e. enzymolysis tortoise plastron collagen peptide extract;
3) by 2kg green tea, be crushed to fineness 2 × 2mm, then add 16 liters of 85 DEG C of hot-water soaks 30 minutes, cool the temperature to 45 DEG C;
4) adopt citric acid adjustment green tea solution pH to 5.0, by the amount of 40,000 U cellulase/1g green tea, slowly add 0.2kg zytase (400,000 U/g), limit edged stirs, and is incubated 2 hours, is heated to 100 DEG C and makes enzyme-deactivating, then filter while hot, obtaining filtrate is green tea extractive liquor;
5) get celestial careless 9kg, Poria cocos 4kg, honeysuckle 1.2kg, dandelion 1.2kg, dried rhizome of rehmannia 1.0kg, Radix Glycyrrhizae 0.8kg, be placed in multi-function extractor and decoct once, add the water of 140L at every turn, decoct 2 hours; Merge decoction liquor, centrifugal filtration, obtains filtrate, namely decocts extract;
6) merge enzymolysis tortoise plastron collagen peptide extract and decoct extract, be concentrated into 80L, add 12kg rock sugar, 4.0kg starch, 0.5kg theanine and green tea extractive liquor, finally add or do not add 0.2kg carragheen, heat while stirring, after gelatinization completely, packing, then through high-temperature sterilization, after cooling, Guiling Jelly finished product is namely obtained.
Embodiment 2
1) 1kg tortoise plastron is cleaned, be crushed to fineness and be less than 2 × 2mm, then drop in the water of 7L and soak 2 hours;
2) add by the amount of 50,000 U protease/1g tortoise plastrons the compound protease be made up of papain and bromelain, wherein papain (800,000 U/g) 0.0125kg and bromelain (800,000 U/g) 0.05kg, limit edged stirs and evenly mixs; Adjust pH to 6.5, be warming up to 45 DEG C, be incubated after 4 hours, be heated to 100 DEG C and make enzyme-deactivating, then filter while hot, obtain filtrate, be i.e. enzymolysis tortoise plastron collagen peptide solution;
3) by 2.8kg green tea powder, add 25 liters of 85 DEG C of hot-water soaks 30 minutes, cool the temperature to 55 DEG C;
4) adjust pH to 4.0, by the amount of 100,000 U cellulase/1g green tea, slowly add 0.7kg zytase (400,000 U/g), limit edged stirs, and is incubated 3 hours, is heated to 100 DEG C and makes enzyme-deactivating, then filter while hot, and obtaining filtrate is green tea extractive liquor.
5) get celestial careless 9.5kg, Poria cocos 4.5kg, honeysuckle 1.5kg, dandelion 1.5kg, dried rhizome of rehmannia 1.4kg, Radix Glycyrrhizae 1.3kg, be placed in multi-function extractor and decoct once, add the water of 180L at every turn, decoct 2 hours; Merge decoction liquor, centrifugal filtration, obtains filtrate, namely decocts extract;
6) merge enzymolysis tortoise plastron collagen peptide extract and decoct extract, be concentrated into 80L, add 12kg rock sugar, 4.5kg starch, 0.7kg theanine and green tea extractive liquor, finally add or do not add 0.2kg carragheen, heat while stirring, after gelatinization completely, packing, then through high-temperature sterilization, after cooling, Guiling Jelly finished product is namely obtained.
Embodiment 3
1) 1.3kg tortoise plastron is cleaned, be crushed to fineness and be less than 2 × 2mm, then drop in the water of 13L and soak 2 hours;
2) add by the amount of 100,000 U enzyme/1g tortoise plastrons the compound protease be made up of papain and bromelain, wherein papain (800,000 U/g) 0.06kg and bromelain (800,000 U enzyme/g) 0.10kg, limit edged stirs and evenly mixs; Adjust pH to 6.5, be warming up to 45 DEG C, be incubated after 4 hours, be heated to 100 DEG C and make enzyme-deactivating, then filter while hot, obtain filtrate, be i.e. enzymolysis tortoise plastron collagen peptide extract;
3) by 2.5kg green tea, pulverize, add 8 liters of 85 DEG C of hot-water soaks 30 minutes, cool the temperature to 40 DEG C;
4) adjust pH to 6.0, by the amount of 80,000 U cellulase/1g green tea, slowly add 0.5kg zytase (400,000 U/g), limit edged stirs, and is incubated 4 hours, is heated to 100 DEG C and makes enzyme-deactivating, then filter while hot, and obtaining filtrate is green tea extractive liquor;
5) get celestial careless 9.3kg, Poria cocos 5kg, honeysuckle 1.4kg, dandelion 1.4kg, dried rhizome of rehmannia 1.2kg, Radix Glycyrrhizae 1.0kg, be placed in multi-function extractor and decoct once, add the water of 200L at every turn, decoct 2 hours; Merge decoction liquor, centrifugal filtration, obtains filtrate, namely decocts extract;
6) merge enzymolysis tortoise plastron collagen peptide extract and decoct extract, be concentrated into 80L, add 12kg rock sugar, 4.8kg starch, 0.9kg theanine and green tea extractive liquor, finally add or do not add 0.2kg carragheen, heat while stirring, after gelatinization completely, packing, then through high-temperature sterilization, after cooling, Guiling Jelly finished product is namely obtained.
Embodiment 4: human experimentation:
4.1 test crowds
Choose 11 suspect self because life and work pressure is excessive, poor sleeping quality, badtempered male volunteers and 19 suffer from the premenstrual syndrome of different situations and feel nervous, tired and gloomy female volunteers for a long time.
4.2 test condition
1) each tester taught without accepting any spirit in 3 months all before testing;
2) name tester used the spiritual regulating drug and health food of being correlated with all before testing in February;
3) tester wants the testing period not according to teaching by any spirit and edible relevant medicine/health product;
4) without health products allergies person or without severe allergy history person;
5) nothing is addicted to drink, take drugs custom person.
4.3 test mode
30 volunteers are often organized 15 people at random, is divided into 2 groups, be respectively control group and test group.
Control group: every day is constant by daily living and diet.
Test group: when constant by daily life, increases the Guiling Jelly 200g being rich in theanine in the edible embodiment of the present invention 1 every day, edible 2 months continuously, the physical condition change of each examination trencherman before and after observation survey test.
4.4 test result
Contrast physical condition change before and after test-meal, result is as follows:
Test group: 5 female subjects represent that stress sense before and after menstrual period and sense of fatigue disappear, and during menstrual period, trick ice-cold sense makes moderate progress; 4 female subjects represent that usual gloomy sense reduces to some extent, and dysmenorrhoea sense reduces, and sleep quality makes moderate progress; 2 female subjects represent that physical condition change is little, without any sensation; 4 male subject represent and to control the emotion in edible period, feel that mental state is gentle to some extent, the anxiety not and hot-tempered sense simultaneously;
Control group: 3 volunteers represent that recent mood is mild, and without gloomy and tension, sleep quality will be got well before comparatively testing; 12 volunteers represent that physical condition is not any change.
Result shows, the Guiling Jelly being rich in theanine of the present invention can effectively regulate brain neuroblastoma material Transfer, improve premenstrual syndrome, extenuate emotional stress and improving water flood quality.
Above better embodiment of the present invention is illustrated, but the invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modifications or replacement under the prerequisite without prejudice to spirit of the present invention, and these equivalent modification or replacement are all included in the application's claim limited range.

Claims (10)

1. be rich in a preparation technology for the Guiling Jelly of theanine, it is characterized in that, comprise the following steps:
1) tortoise plastron cleaned, after fragmentation, drop in water and soak 2 ~ 4 hours;
2) add protease by the amount of 5 ~ 100,000 U protease/1g tortoise plastrons, stir and evenly mix, enzymolysis is after 1 ~ 4 hour, and heating makes protease deactivation, then filters while hot, obtains enzymolysis tortoise plastron collagen peptide extract;
3) green tea is dropped in the hot water of 85 DEG C and soak 30 minutes;
4) add cellulase, enzymolysis 2-4 hour by the amount of 4-10 ten thousand U cellulase/1g green tea, heating makes cellulase deactivation, obtains the green tea extractive liquor after enzymolysis;
5) take celestial grass, Poria cocos, honeysuckle, dandelion, Radix Glycyrrhizae and dried rhizome of rehmannia Six-element raw material, drop in water, decoct 1-2 time, each decoction 1-2 hour; Merge decoction liquor, centrifugal filtration, draws decoction extract;
6) merge enzymolysis tortoise plastron egg peptide extract and decoct extract, adding theanine, flavouring, stabilizing agent and green tea extractive liquor after concentrated, after heating is stirred to gelatinization, packing, sterilizing, draw Guiling Jelly finished product.
2. preparation technology according to claim 1, is characterized in that: cleaned by tortoise plastron in step 1) and be crushed to fineness and be less than or equal to 2 × 2mm, the consumption of water be the 5-10 of tortoise plastron weight doubly.
3. preparation technology according to claim 1, is characterized in that: step 2) described in protease be papain and bromelain.
4. preparation technology according to claim 3, described in its feature, the consumption of papain and bromelain is: make its enzyme ratio alive be 1:(1-5).
5. preparation technology according to claim 1, is characterized in that: in step 3), and described green tea is green tea or green tea powder, and wherein the fineness of green tea is 2 × 2mm; The consumption of water is 8-10 times of green tea weight.
6. preparation technology according to claim 1, is characterized in that: in step 4), and described cellulase is zytase.
7. preparation technology according to claim 6, is characterized in that: during enzymolysis, and pH value is 4.0 ~ 6.0, and temperature is 40 ~ 55 DEG C, uses citric acid adjusted to ph.
8. preparation technology according to claim 1, is characterized in that: in step 5), and the consumption of water is 8 ~ 10 times of Six-element raw material weight.
9. the preparation technology according to any one of claim 1-8, it is characterized in that, described Guiling Jelly is prepared from by the raw material of following weight portion, tortoise plastron 5-15 part, green tea 20-30 part, theanine 5-10 part, celestial careless 90-98 part, honeysuckle 12-16 part, Fu Ling 40-60 part, dandelion 12-16 part, dried rhizome of rehmannia 10-15 part, Radix Glycyrrhizae 8-15 part; Described flavouring is rock sugar, and described stabilizing agent is carragheen and/or starch.
10. preparation technology according to claim 9, is characterized in that, the ratio of described starch and carragheen is 20-24:1.
CN201410739803.9A 2014-12-08 2014-12-08 Preparation process of tortoise shell and poria cocos jelly rich in theanine Pending CN104489433A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104839522A (en) * 2015-04-17 2015-08-19 邹健 Nutrition and health Guiling jelly and preparation method thereof
CN106360725A (en) * 2016-11-10 2017-02-01 湄潭县京贵茶树花产业发展有限公司 Extraction method of antioxidant from tea flowers
CN112914093A (en) * 2021-03-17 2021-06-08 国健药业(深圳)集团有限公司 Tortoise jelly for refreshing and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102007993A (en) * 2010-11-11 2011-04-13 杭州大茗堂生物科技有限公司 Sugarless theanine buccal tablets and preparation method thereof
CN102429221A (en) * 2011-10-20 2012-05-02 董燕花 Camellia chrysantha tortoise jelly
CN103005246A (en) * 2012-12-28 2013-04-03 江门市生和堂食品有限公司 Tortoise shell collagen peptide-containing tortoise herb jelly and preparation process thereof
CN103621854A (en) * 2013-11-14 2014-03-12 江门市生和堂食品有限公司 Heart nourishing tortoise jelly and preparation process thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102007993A (en) * 2010-11-11 2011-04-13 杭州大茗堂生物科技有限公司 Sugarless theanine buccal tablets and preparation method thereof
CN102429221A (en) * 2011-10-20 2012-05-02 董燕花 Camellia chrysantha tortoise jelly
CN103005246A (en) * 2012-12-28 2013-04-03 江门市生和堂食品有限公司 Tortoise shell collagen peptide-containing tortoise herb jelly and preparation process thereof
CN103621854A (en) * 2013-11-14 2014-03-12 江门市生和堂食品有限公司 Heart nourishing tortoise jelly and preparation process thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104839522A (en) * 2015-04-17 2015-08-19 邹健 Nutrition and health Guiling jelly and preparation method thereof
CN106360725A (en) * 2016-11-10 2017-02-01 湄潭县京贵茶树花产业发展有限公司 Extraction method of antioxidant from tea flowers
CN112914093A (en) * 2021-03-17 2021-06-08 国健药业(深圳)集团有限公司 Tortoise jelly for refreshing and preparation method thereof

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Application publication date: 20150408