CN104488981A - Application of essential oil of lemon and eucalyptus leaves - Google Patents
Application of essential oil of lemon and eucalyptus leaves Download PDFInfo
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- CN104488981A CN104488981A CN201410763062.8A CN201410763062A CN104488981A CN 104488981 A CN104488981 A CN 104488981A CN 201410763062 A CN201410763062 A CN 201410763062A CN 104488981 A CN104488981 A CN 104488981A
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- essential oil
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses an application of essential oil of lemon and eucalyptus leaves. The essential oil is taken as a mosquito repellent. Compared with other eucalyptus leaves essential oil, the essential oil of lemon and eucalyptus leaves disclosed by the invention has the advantages of relatively good insecticidal activity and relatively high mosquito fumigating and killing rate.
Description
Technical field
The present invention relates to a kind of application of lemon scented gum leaf essential oil.
Background technology
Eucalyptus is the general name that Myrtaceae (Myrtaceae) eucalyptus belongs to (Eucalyptus) plant, is grown on Perenniporia martius.There is drought-resistant barren, fast growing, wide adaptability, the degeneration-resistant good characteristic such as by force, become one of large reproducting tree species (eucalyptus, pine, poplar) in the world three.The chief species in Guangxi are Eucalyptus urophylla and hybridization seeds thereof, and as Eucalyptus urophylla-grandis, tail alpine ash, clone such as tail circle eucalyptus, tail eucalyptus camaldulensis etc., its abundant eucalyptus leaves essential oil resource needs to develop.
Summary of the invention
The technical problem to be solved in the present invention overcomes existing defect, provides a kind of application of lemon scented gum leaf essential oil.
In order to solve the problems of the technologies described above, the invention provides following technical scheme:
The application of lemon scented gum leaf essential oil, described essential oil is as mosquito fumigant.
The extracting method of described lemon scented gum leaf essential oil, comprises the steps:
Being placed in volatile oil extractor by eucalyptus leaves chopping, is that 1:4 ~ 1:12 adds water, soaking at room temperature 1-8 hour by feed liquid, then heating and refluxing extraction 1-6 hour.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein is only for instruction and explanation of the present invention, is not intended to limit the present invention.
embodiment 1
Take the eucalyptus citriodora leaf that 100g dries in the shade naturally, leaf is cut into 1 ~ 2cm
2left and right, the leaf of chopping is placed in volatile oil extractor, is that 1:8 adds water by solid-liquid ratio, and after soaking at room temperature 6h, heating and refluxing extraction essential oil, extraction time is 70 minutes, extracts completely after profit is separated completely, to obtain essential oil.
embodiment 2
Take the eucalyptus citriodora leaf that 100g dries in the shade naturally, leaf is cut into 1 ~ 2cm
2left and right, the leaf of chopping is placed in volatile oil extractor, is that 1:6 adds water by solid-liquid ratio, and after soaking at room temperature 2h, heating and refluxing extraction essential oil, extraction time is 30 minutes, extracts completely after profit is separated completely, to obtain essential oil.
mosquito smoked kill is tested
1.1 for examination mosquito
Test mosquito used is the female mosquito that culex pipiens fatigans (Culex pipiens quinquefasciatus) 3 ~ 5 ages in days are not sucked blood, draw from Municipal Disease Control and Prevention Center, Liuzhou, be national standard Household insecticide evaluating drug effect insect (GB13917.6-92), raised by insectarium of Huali Domestic Articles Industry Co., Ltd., Liuzhou and go down to posterity.Mosquito growth conditions is: temperature 26 DEG C of scholars 1 DEG C, relative moisture 55% scholar 10%, photoperiod: 12h/12h.
1.1.3 experimental technique
(1) preparation of solution to be measured
With 5 kinds of eucalyptus leaf essential oils such as eucalyptus camaldulensis, E. dunnii, Eucalyptus urophylla, eucalyptus citriodora, blue gums for solute, to take acetone as solvent dose volume mark be 0.4%, 0.8%, 1.6%, 3.2%, 4.8%, 6.4%, 9.6% eucalyptus leaf essential oil solution; With principal monomer-1 of eucalyptus leaf essential oil, 8-cineole, australene, nopinene, p-cymene, alpha-terpineol, citronellal, citronellol are solute, to take acetone as solvent dose volume mark be 0.0125%, 0.025%, 0.05%, 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 3.2%, 6.4%, 6.6%, 7.2%, 8%, 9.6%, 12.8% solution.
(2) fumigation effect experiment
Stifling lethal in dense (LC50) and the stifling lethal time of 50 (LT50) be the important indicator whether measurement determinand has product development value.Adopt 5 kinds of eucalyptus leaf essential oils and 1 such as triangular flask airtight fumigating system research eucalyptus camaldulensis, E. dunnii, Eucalyptus urophylla, eucalyptus citriodora, blue gum herein, 7 kinds of monomers such as 8-cineole, australene, nopinene, p-cymene, alpha-terpineol, citronellal, citronellol to the smoked kill effect of culex pipiens fatigans, determine above-mentioned various eucalyptus leaf essential oil and monomer to examination worm stifling lethal in dense (LC50) and the stifling lethal time of 50 (LT50).
This experiment test condition is: temperature 25 DEG C of scholars 1 DEG C, relative moisture 50% scholar 5 DEG C.
Stifling lethal in the mensuration of dense (LC50): with reference to Jiang Zhili, raise frequently and the research method (the airtight fumigating system of triangular flask) of Liu Yu minister in ancient times 1 carry out optimal inspection, the female adult pest of 15 2 ~ 3 age in days culex pipiens fatigans is accessed in 250 mL triangular flasks, triangular flask is airtight with the cork being surrounded by gauze.Cork is fixed one piece of filter paper bar (lcm × 3cm), on filter paper bar, drips 10 μ L for after examination essential oil with micropipettor, build cork rapidly.Check respectively in 0.5,1,2,4,8,24 h and record dead borer population.Each concentration repeats 3 times, repeats using the pure acetone of same dose as blank at every turn.When control group lethality is greater than 5%, use Abbott formula correction; When control group lethality is greater than 20%, test is reformed.
The mensuration of the stifling lethal time of 50 (LT50): be 6.4% each eucalyptus leaf essential oil and monomer suffocating treatment culex pipiens fatigans with concentration, processing method is the same, checks its death toll every 1min.Survey data acquisition probit analysis and calculate, calculate LT50 and virulence regression equation (LT-p), and c2 inspection carried out to virulence regression equation.Namely mosquito overturning is considered as death.The poisoning symptom of the worm of tracing observation examination simultaneously.When control group lethality is greater than 5%, use Abbott formula correction; When control group lethality is greater than 20%, test is reformed.
1.2 results and analysis
1.2.1 culex pipiens fatigans poisoning symptom observed result
During with 6.4% eucalyptus leaf essential oil process culex pipiens fatigans, mosquito is very excited at the beginning, does not ground always, and after having flown a period of time, just stop to fall, some mosquito overturnings, fall backwards with hands and legs in the air; Some mosquitoes are benumbed, slow in action; Then peace and quiet are motionless on all fours to also have some mosquitoes, finally dead.The symptom that the culex pipiens fatigans of each essential oil process shows is very nearly the same, and the time only occurred has any different because of the difference of essential oil kind.
1.2.2 eucalyptus leaf essential oil is to culex pipiens fatigans smoked kill virulence
Adopt 5 kinds of eucalyptus leaf essential oils such as triangular flask airtight fumigating system research eucalyptus camaldulensis, E. dunnii, Eucalyptus urophylla, eucalyptus citriodora, blue gum to the smoked kill effect of culex pipiens fatigans, survey data acquisition probit analysis and calculate LC50 and virulence regression equation (LC-p), and c2 inspection is carried out to virulence regression equation.LC50 value is less, shows that it is stronger to the lethal effect of culex pipiens fatigans.The fumigation effect of 5 kinds of eucalyptus leaf essential oils to culex pipiens fatigans the results are shown in Table 1-1 ~ 1-2.Each virulence regression equation is checked all to tally with the actual situation through c2 (f=3, P=0.05).
Table 1-1 5 kinds of eucalyptus leaf essential oils and main component thereof are to the smoked killing effect (fumigation time is 0.5h) of culex pipiens fatigans
Note: " x " represents log concentration; " y " representation theory probit value.
Table 1-2 5 kinds of eucalyptus leaf essential oils and main component thereof are to the smoked killing effect (fumigation time is 24h) of culex pipiens fatigans
Note: " x " represents log concentration; " y " representation theory probit value.
1.2.3 eucalyptus leaf essential oil is to the fumigation time effect of culex pipiens fatigans
The airtight fumigating system of triangular flask is adopted to have studied 5 kinds of eucalyptus leaf essential oils such as eucalyptus camaldulensis, E. dunnii, Eucalyptus urophylla, eucalyptus citriodora, blue gum to the time effect of culex pipiens fatigans smoked kill effect, survey data acquisition probit analysis and calculate LT50 and virulence regression equation (LT-p), and c2 inspection is carried out to virulence regression equation.LT50 is less, and its smoked kill speed is faster.Experimental result is in Table 1-3.Each virulence regression equation is checked all to tally with the actual situation through c2 (f=3, P=0.05).
Table 1-3 5 kinds of eucalyptus leaf essential oils and main component thereof are to the fumigation time effect (LT of culex pipiens fatigans
50)
Note: " x " represents log concentration; " y " representation theory probit value.Laboratory sample concentration is 6.4%.
As can be seen from upper experiment, compared with other eucalyptus leaves essential oil, lemon scented gum leaf essential oil all has clear superiority to the insecticidal activity of mosquito and smoked kill speed.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (1)
1. the application of lemon scented gum leaf essential oil, described essential oil is as mosquito fumigant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410763062.8A CN104488981A (en) | 2014-12-13 | 2014-12-13 | Application of essential oil of lemon and eucalyptus leaves |
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| CN201410763062.8A CN104488981A (en) | 2014-12-13 | 2014-12-13 | Application of essential oil of lemon and eucalyptus leaves |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110537504A (en) * | 2019-09-20 | 2019-12-06 | 山东龙昌动物保健品有限公司 | Eucalyptus multicastus essential oil and application of compound microbial inoculum thereof in preparation of insect repellent for marine fishes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1860872A (en) * | 2005-05-10 | 2006-11-15 | 大日本除虫菊株式会社 | Flying pest vermifuge composition |
| US20130084347A1 (en) * | 2011-09-30 | 2013-04-04 | Steven Fred Salomon | Natural insect repellent compositions |
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2014
- 2014-12-13 CN CN201410763062.8A patent/CN104488981A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1860872A (en) * | 2005-05-10 | 2006-11-15 | 大日本除虫菊株式会社 | Flying pest vermifuge composition |
| US20130084347A1 (en) * | 2011-09-30 | 2013-04-04 | Steven Fred Salomon | Natural insect repellent compositions |
Non-Patent Citations (3)
| Title |
|---|
| 周贤闯: "桉叶精油对蚊虫的驱避与毒杀作用研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
| 左娅 等: "低浓度驱蚊产品无碍健康", 《人民日报》 * |
| 江湖 等: "蚊害生物防治方法研究进展", 《生物学教学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110537504A (en) * | 2019-09-20 | 2019-12-06 | 山东龙昌动物保健品有限公司 | Eucalyptus multicastus essential oil and application of compound microbial inoculum thereof in preparation of insect repellent for marine fishes |
| CN110537504B (en) * | 2019-09-20 | 2021-11-02 | 山东龙昌动物保健品有限公司 | Eucalyptus multicastus essential oil and application of compound microbial inoculum thereof in preparation of insect repellent for marine fishes |
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