CN104473977B - A kind of Cordyceps militaris water extract, preparation method and applications - Google Patents
A kind of Cordyceps militaris water extract, preparation method and applications Download PDFInfo
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- CN104473977B CN104473977B CN201410660797.8A CN201410660797A CN104473977B CN 104473977 B CN104473977 B CN 104473977B CN 201410660797 A CN201410660797 A CN 201410660797A CN 104473977 B CN104473977 B CN 104473977B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The present invention provides a kind of preparation method of Cordyceps militaris water extract, using aboundresources, cheap water as solvent, reaches reduction production cost, avoids the purpose of organic solvent pollution.Obtained Cordyceps militaris water extract can be used for preparing reduction uric acid concentration, improve medicine, health products or the assistant medicament of gout illness by this method.
Description
【Technical field】
The present invention relates to a kind of Cordyceps militaris water extract, preparation method and applications, belong to health products and the field of Chinese medicines.
【Background technology】
Goat seriously perplexs human lives, and the epidemiological study of New Zealand, the U.S. and China shows that gout illness becomes
Obtain more and more universal, the incidence of disease is about 2%, and constantly raised.Wherein 65 years old and above crowd's incidence highest, the morbidity of male
Rate is also higher twice than the women incidence of disease.Goat is the result of hyperuricemia development, is due to that internal uric acid concentration is higher than blood
Liquid solvability (>360 μm of ol/L), cause sodium urate crystals to be deposited in soft tissue, joint, these depositing crystallines cause soft group
Knit, inflammatory has an intense pain repeatedly for joint and bone tissue, seriously reduces patients ' life quality.
Goat is divided into acute and chronic two kinds.It is anti-including non-steroidal for the sick medicine of acute gout in the market
Scorching medicine, colchicine, corticotropin or steroids, these medicines, which are mainly, acts on inflammatory factor, reduces patient
Pain.For chronic gout disease, management blood uric acid levels are crucial.At present, for reducing the medicine of uric acid concentration in human body
Mainly have：Allopurinol, medicine for improving uric acid excretion and diuretic, including the uric acid that Benzbromarone, uricase, PEG are modified
Enzyme etc., these medicines to liver and kidney metabolic organ because there is stronger side effect to prevent the patient with hepatopathy and nephrosis from making
With while these medicines easily trigger intestines and stomach and cardiovascular disease.This cause with hypertension, congestive heart failure, gastritis/
The patient with gout of ulcer or renal insufficiency, which takes said medicine, generally has higher risk.Chinese Herbs are good, side effect
Low, its advantage gradually shows, thus clinically widely uses.
For goat, there is text and report Cordyceps militaris ethanol extract with reduction uric acid, alleviation goat.But
It is its preparation method complexity, cost height, and is extracted using organic solvent, there is the problem of organic solvent pollution is big.
【The content of the invention】
In view of the above-mentioned problems, the present invention be a kind of preparation method of Cordyceps militaris water extract is provided, using aboundresources,
Cheap water reaches reduction production cost, avoids the purpose of organic solvent pollution as solvent.Specifically include following step
Suddenly：
(1) dry fruiting bodies of cordyceps militaris is crushed；
(2) fruiting bodies of cordyceps militaris after crushing is placed in the water of 5-25 times of weight, extraction 1-2 is small at 60-100 DEG C
When, filtered with 400 mesh filter screens；
(3) with the nutriment repeated with step (2) identical condition in extraction gained filter residue.
(4) vacuum distillation is concentrated into 250-350mL after the filtrate obtained by step (2) and (3) is merged；
(5) concentrate obtained by step (4) is freezed and obtains Cordyceps militaris water extract.
It is preferred that, the extraction process in above-mentioned steps (2) is ultrasonic extraction, and temperature is preferably 80 DEG C.
Obtained Cordyceps militaris water extract, is the nutritional ingredient extracted from fruiting bodies of cordyceps militaris, contains according to the method described above
Crude protein, crude fat, carbohydrate, mannitol, cordyceps sinensis oil, cordycepin, sterols, ucleosides, amino acid, peptides, polysaccharide,
Monose, organic acid, vitamin and inorganic elements, wherein, mannitol content is：4-7%, polyoses content is 20-25%, and nucleosides contains
Measure as 0.25-0.35%.
Take Cordyceps militaris water extract made from the above method in vivo test uric acid, as a result show to use Cordyceps militaris water extract
Continuous gavage 7 days, can be reduced to normal mouse uric acid level by the blood uric acid concentration of high lithemia mouse.
Therefore, preparing reduction uric acid concentration it is a further object of the present invention to provide above-mentioned Cordyceps militaris water extract, improving pain
Application in medicine, health products or the assistant medicament of wind illness.
It is a further object of the present invention to provide a kind of medicine for treating goat, it is characterised in that the medicine contains above-mentioned
Cordyceps militaris water extract.
The beneficial effects of the present invention are：
1st, the preparation technology of Cordyceps militaris water extract of the present invention is simple, and cost is low；
2nd, the present invention using water as solvent extraction Cordyceps militaris water extract, the problem of in the absence of organic solvent pollution, safety
Environmental protection；
3rd, there is Cordyceps militaris water extract of the present invention significant anti-trioxypurine to act on, and can be used in preparing the medicine for alleviating gout illness
Thing.
【Brief description of the drawings】
Fig. 1：Uric acid concentration in each group mice serum
【Embodiment】
It is explained further the present invention with reference to embodiments, but embodiment does not do any type of limit to the present invention
It is fixed.
Embodiment 1
First, the preparation of Cordyceps militaris water extract (ETCM)
Fruiting bodies of cordyceps militaris 100g is taken, is added after being crushed using pulverizer in conical flask, and adds 2L distilled water, will be mixed
Thing is filtered after water-bath is extracted 2 hours at 80 DEG C using 400 mesh filter screens.Extract experiment to be repeated once, after gained filtrate is merged
Vacuum distillation is concentrated into 300mL, and lyophilized to obtain Cordyceps militaris water extract 53g, yield is 53%.
2nd, each component assay in Cordyceps militaris water extract
Mannitol is determined：Mannitol content is determined using the chromogenic reaction of mannitol and sodium metaperiodate and Nash reagents.Its
Middle agents useful for same is formulated as follows：Nash reagents：Take ammonium acetate 150g, with appropriate distilled water dissolve after, add 2mL glacial acetic acid and
2mL acetylacetone,2,4-pentanediones, 1000mL is dissolved to distilled water；0.015mol/L sodium metaperiodates：Sodium metaperiodate 3.2g is taken to be dissolved in 1000mL
0.12mol/L HCI in；0.1%L- rhamnoses：L- rhamnose 0.1g are taken, are dissolved in 100mL distilled water.Mannitol standard items
Solution：Mannitol standard items 100mg is taken, is dissolved in 100mL distilled water.
Added in test tube in testing sample solution 0.03mL, benefit distilled water to 1mL, blank control pipe and add distillation
Mannitol standard solution 1mL is added in water 1mL, titer control tube, then respectively in experiment tube, blank control pipe and standard
0.015mol/L sodium metaperiodate liquid 1mL are added in liquid control tube, room temperature is placed 10 minutes after mixing, and each test tube adds 0.1% mouse
Lee sugar 2mL, after fully mixing, adds Nash reagents 4mL, 53 DEG C of water-baths are incubated 15min.UV, visible light light splitting light is used after cooling
Degree meter determines its absorption value at 413nm, and the mannitol content in sample is calculated according to the absorbance value of mannitol standard liquid.
It is 4% that this method, which measures mannitol content in the sample of embodiment 1,.
Sulfuric acid-phynol method is used to measure in the sample of embodiment 1 polyoses content for 22%.
It is 0.29% to measure nucleosides content in the sample of embodiment 1 using HPLC.
3rd, Cordyceps militaris water extract anti-trioxypurine is tested
60 male SPF kunming mices (20 ± 2g) are taken, 6 groups are randomly divided into：Normal group, hyperuricemia model pair
According to group, positive controls 1, positive controls 2, ETCM low dose groups, ETCM high dose groups.Except Normal group intraperitoneal injection and
Outside gavage physiological saline, Oxonic Acid sylvite is injected intraperitoneally according to 100mg/kg/d dosage in other groups, while gavage 600mg/kg/
The hypoxanthine modeling of d dosage.Water is can't help in the previous hour fasting of modeling, 1 hour after modeling, positive controls gavage 5mg/
The allopurinol (AL) of kg/d dosage, the allopurinol (AL) of the gavage 10mg/kg/d dosage of positive controls 2, Cordyceps militaris water extraction
The Cordyceps militaris water extract (ETCM) of thing low dose group gavage 50mg/kg/d dosage, Cordyceps militaris water extract high dose group gavage
The Cordyceps militaris water extract (ETCM) of 200mg/kg/d dosage, then gavage is identical with hyperuricemia model control group for Normal group
The physiological saline of volume, continuous 7 days.7th day gastric infusion is after 1 hour, and anesthesia plucks eyeball and takes blood, using centrifuge in 3500r/
Under min speed centrifuge the isolated serum of 10min, detect uric acid in serum concentration, as a result as shown in Figure 1 (* represent with normally it is right
Compared according to group, significant difference (P<0.01)；# represented compared with model control group, significant difference (P<0.01)).
From figure 1 it appears that Cordyceps militaris water extract of the present invention has good anti-trioxypurine effect, its anti-trioxypurine effect
High lithemia model group blood uric acid concentration can be made to be reduced to normal mouse uric acid level.In addition, Cordyceps militaris is used as integration of drinking and medicinal herbs
Fungi, to human body not damaged, its water extract can be used for preparing anti-trioxypurine, the medicine for improving gout, health products or
In assistant medicament.
Embodiment 2
Fruiting bodies of cordyceps militaris 100g is taken, is added after being crushed using pulverizer in conical flask, and adds 500mL distilled water, will
Mixture ultrasonic extraction at 60 DEG C is filtered after 2 hours using 400 mesh filter screens.Extract experiment to be repeated once, gained filtrate is closed
And rear vacuum distillation is concentrated into 250mL, lyophilized to obtain Cordyceps militaris water extract 39g, yield is 39%.
It is 7%, polyoses content to measure mannitol content in the Cordyceps militaris water extract according to the detection method described in embodiment 1
For 20%, nucleosides content is 0.35%.
Embodiment 3
Fruiting bodies of cordyceps militaris 100g is taken, is added after being crushed using pulverizer in conical flask, and adds 2.5L distilled water, will be mixed
Compound is filtered after extraction being decocted at 100 DEG C 2 hours using 400 mesh filter screens.Extract experiment to be repeated once, gained filtrate is merged
Vacuum distillation is concentrated into 350mL afterwards, and lyophilized to obtain Cordyceps militaris water extract 45g, yield is 45%.
It is 5.4% to measure mannitol content in the Cordyceps militaris water extract according to the detection method described in embodiment 1, and polysaccharide contains
Measure as 25%, nucleosides content is 0.25%.
Embodiment 4
Fruiting bodies of cordyceps militaris 100g is taken, is added after being crushed using pulverizer in conical flask, and adds 1.5L distilled water, will be mixed
Compound is filtered after water-bath is extracted 2 hours at 90 DEG C using 400 mesh filter screens.Extract experiment to be repeated once, gained filtrate is merged
Vacuum distillation is concentrated into 300mL afterwards, and lyophilized to obtain Cordyceps militaris water extract 49g, yield is 49%.
It is 6.2% to measure mannitol content in the Cordyceps militaris water extract according to the detection method described in embodiment 1, and polysaccharide contains
Measure as 24%, nucleosides content is 0.33%.
Claims (6)
1. a kind of preparation method for the Cordyceps militaris water extract that there is reduction uric acid to act on, it is characterised in that comprise the following steps：
(1) dry fruiting bodies of cordyceps militaris is crushed；
(2) fruiting bodies of cordyceps militaris after crushing is placed in the water of 5-25 times of weight, extracted 1-2 hours at 60-100 DEG C, used
400 mesh filter screens are filtered；
(3) with the nutriment repeated with step (2) identical condition in extraction gained filter residue；
(4) vacuum distillation is concentrated into 250-350mL after the filtrate obtained by step (2) and (3) is merged；
(5) concentrate obtained by step (4) is freezed.
2. the preparation method of Cordyceps militaris water extract according to claim 1, it is characterised in that carrying in the step (2)
It is 80 DEG C to take temperature.
3. the preparation method of Cordyceps militaris water extract according to claim 1, it is characterised in that carrying in the step (2)
It is ultrasonic extraction to take process.
4. Cordyceps militaris water extract made from the preparation method of the Cordyceps militaris water extract according to any one of claim 1-3,
In the Cordyceps militaris water extract, mannitol content is 4-7%, and polyoses content is 20-25%, and nucleosides content is 0.25-0.35%.
5. the Cordyceps militaris water extract described in claim 4 is preparing reduction uric acid concentration, is improving medicine, the health products of gout illness
Or the application in assistant medicament.
6. a kind of medicine for treating goat, it is characterised in that the medicine contains the Cordyceps militaris water extraction described in claim 4
Thing.
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| CN105087332A (en) * | 2015-08-31 | 2015-11-25 | 遵义鸿霖生物技术有限公司 | Preparing process of soy sauce flavor type cordyceps militaris wine |
| CN105876777A (en) * | 2016-04-12 | 2016-08-24 | 周安琪 | Food having effect of reducing uric acid and preparation technology thereof |
| CN105943958A (en) * | 2016-06-03 | 2016-09-21 | 黑龙江省玄鸟生物科技股份有限公司 | Inonotus obliquus composite solid particles for treating gout and preparing method thereof |
| CN107050067A (en) * | 2016-11-02 | 2017-08-18 | 仪征康海农业发展有限公司 | A kind of industrialized process for preparing of Cordyceps militaris water soluble powder |
| CN106692158A (en) * | 2017-01-18 | 2017-05-24 | 广东省微生物研究所 | Application of vitamin B1, cordycepic acid and riboflavin and composition thereof in uric acid-lowering drugs |
| CN108042627B (en) * | 2017-12-28 | 2021-03-26 | 广东粤微食用菌技术有限公司 | Composition for treating hyperuricemia and preparation method and application thereof |
| CN108403729B (en) * | 2018-05-14 | 2021-09-14 | 广东省微生物研究所（广东省微生物分析检测中心） | Preparation method of agrocybe cylindracea extract and application of agrocybe cylindracea extract in preparation of uric acid reducing medicine |
| CN109170424A (en) * | 2018-08-22 | 2019-01-11 | 江苏国桢生物科技有限公司 | A kind of cordycepin beverage and preparation method thereof |
| CN108992471A (en) * | 2018-08-28 | 2018-12-14 | 广东粤微生物科技有限公司 | The preparation method of selenium-enriched cordceps militaris extract and its application in preparation inhibiting hyperuricemia and/or oxidation resistant drug or food |
| CN109329671A (en) * | 2018-11-15 | 2019-02-15 | 杨齐辉 | A kind of edible drink product and preparation method thereof for treating gout |
| CN109553647A (en) * | 2018-12-26 | 2019-04-02 | 中国科学院西北高原生物研究所 | The extracting method of metabolite, additive |
| CN109875031A (en) * | 2019-01-25 | 2019-06-14 | 常德炎帝生物科技有限公司 | A kind of complex method for improveing Cordyceps militaris effect component extract |
| CN110448579A (en) * | 2019-09-10 | 2019-11-15 | 北京汉藏金生物科技有限公司 | A kind of quick anti-trioxypurine formula |
| CN110916200A (en) * | 2019-12-02 | 2020-03-27 | 广东微量元素生物科技有限公司 | Uric acid reducing composition and preparation method thereof |
| CN114224957A (en) * | 2021-12-30 | 2022-03-25 | 仙乐健康科技股份有限公司 | Composition with effect of inhibiting uric acid generation and preparation method thereof |
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| CN1075067A (en) * | 1992-02-02 | 1993-08-11 | 陈顺志 | The preparation method of north cordyceps sinensis beverage |
| CN101648009B (en) * | 2009-08-31 | 2012-05-23 | 湖北泰榕药业有限公司 | Method for preparing cordycepin SOD medicinal granules |
| CN102885178B (en) * | 2012-09-29 | 2014-11-12 | 楚雄州百草岭药业发展有限公司 | Cordyceps militaris instant tea and preparation method thereof |
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Application publication date: 20150401 Assignee: Guangdong Meili engine Brand Management Co.,Ltd. Assignor: GUANGDONG INSTITUTE OF MICROBIOLOGY Contract record no.: X2020980006416 Denomination of invention: Water extract, preparation method and application of Cordyceps militaris Granted publication date: 20170912 License type: Common License Record date: 20200924 |
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