CN104450847A - Preparation method and application of Huidouba crude polypeptide - Google Patents
Preparation method and application of Huidouba crude polypeptide Download PDFInfo
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- CN104450847A CN104450847A CN201410782005.4A CN201410782005A CN104450847A CN 104450847 A CN104450847 A CN 104450847A CN 201410782005 A CN201410782005 A CN 201410782005A CN 104450847 A CN104450847 A CN 104450847A
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Abstract
The invention discloses a method for preparing a Huidouba crude polypeptide. The method comprises the following steps: extracting Huidouba crude proteins, performing enzymolysis and purifying. The Huidouba crude proteins are hydrolyzed by utilizing enzymes, so that toxic and side effects brought by chemical reagents are avoided, and the Huidouba polypeptide yield can be improved. The invention also provides application of the prepared Huidouba polypeptide in the preparation of medicines for reducing blood sugar. The proper protease and enzymolysis conditions are optimized, the Huidouba polypeptide is low in cost, simple in process, short in period, high in controllability and high in yield, and large-scale industrial production can be realized.
Description
Technical field
The invention belongs to biomedicine field, be specifically related to preparation and the application thereof of the thick polypeptide of a kind of HUIDOUBA.
Background technology
Diabetes are the absolute or relative hyposecretion of Regular Insulin and chronic generalized all the life metabolic diseases of the disorderly class of body metabolism that causes in the body due to people.Present diabetes have a strong impact on the healthy of the mankind, the annual diabetic subject in the whole world constantly to increase and the age of ill crowd also in continuous decline, present the trend of becoming younger.Market has medicine such as biguanides, the glycosidase enzyme medicine etc. of a lot for the treatment of diabetes, but these medicines of human body long-term taking have certain toxic side effect.Obtain the focus that efficient, safe antihypelipidemic product becomes various countries expert research recent years.Wherein blood sugar lowing polypeptide is one of natural ofhypoglycemic medicine, is the one of biologically active polypeptides.
Tibetan medicine HUIDOUBA is the nest that a kind of cave red spider builds up as starting material with the silk of oneself, this red spider lives in the old tea grove of height above sea level more than 1000 meters, the cloth bag of the outer image grey of nest that it builds up, cave place depends on the limb of old tea tree, bottom is then buried in cave, and whole nest mixes earth and dead twigs and withered leaves, therefore there is earth and the dead twigs and withered leaves of more redness or yellow on surface.This medicine has elimination, diabetes-alleviating symptom, effect of replenishing qi to invigorate the spleen; Metabolism wadding can be corrected disorderly, prevent diabetic complication, be mainly used in treating type ii diabetes, therefore thought the best folk prescription of folk therapy diabetes by everybody.The essential substance of HUIDOUBA is spider silk, and spider silk contains a large amount of silk-proteins.Someone analyzes the therapeutic action of the various activeconstituents of HUIDOUBA to type ii diabetes, the main component obtaining HUIDOUBA treatment type ii diabetes is protein component, but does not analyze the concrete material playing the effect for the treatment of type ii diabetes and study.The method preparing polypeptide conventional is at present chemical method, and this method also exists long reaction time, and hydrolytic process is wayward, and has the residual of chemical reagent, knows from experience generation detrimentally affect, may destroy the biological activity of polypeptide simultaneously to people.
Summary of the invention
Goal of the invention: for solving problems of the prior art, the invention provides preparation and the application thereof of the thick polypeptide of a kind of HUIDOUBA, by the HUIDOUBA polypeptide utilizing protease hydrolysis HUIDOUBA albumen to obtain, there is good blood sugar reducing function.
Technical scheme: for realizing above-mentioned technical purpose, the invention provides the preparation method of the thick polypeptide of a kind of HUIDOUBA, comprising the steps:
(1) extraction of HUIDOUBA crude protein: pulverizing HUIDOUBA raw material is joined in phosphoric acid buffer and soaks, extract under Ultrasonic Conditions, centrifugal, get supernatant liquor and lyophilize, obtain HUIDOUBA crude protein powder;
(2) enzymolysis: HUIDOUBA crude protein powder step (1) obtained is mixed with the aqueous solution, adds proteolytic enzyme and carries out enzyme digestion reaction, obtain the enzymolysis solution of HUIDOUBA crude protein to it;
(3) purifying: the enzymolysis solution that step (2) obtains to go out enzyme, centrifugal, micro-filtration through boiling water bath, the ultra-filtration centrifuge tube of the filtrate different molecular weight obtained carries out centrifugal treating, the polypeptide liquid of the different molecular weight obtained is carried out concentrated and lyophilize, obtain the thick polypeptide of HUIDOUBA.
Particularly, in step (1), in ultrasonic power 700 ~ 900W, ultrasonic time 45-60min, temperature 50-60 DEG C, pH 6-8 and liquid ratio (45 ~ 60): extract HUIDOUBA albumen under the condition of 1mL/g, at the centrifugal 10 ~ 20min of 3000 ~ 5000r/min after question response terminates, supernatant concentration lyophilize, cryodesiccated temperature is-50 DEG C.
In step (2), described proteolytic enzyme is any one in Sumizyme MP, neutral protease, trypsinase, papoid, compound protease and flavor protease.
In step (2), the consumption of described proteolytic enzyme is 4000 ~ 6000U/g, and HUIDOUBA crude protein concentration is 5 ~ 7wt%, and the temperature of enzymolysis is 30 ~ 50 DEG C, and the time of enzymolysis is 2 ~ 4h, and the pH of enzymolysis is 6 ~ 8.
In step (3), the go out condition of enzyme of boiling water bath is: enzymolysis solution is placed in boiling water bath and keeps 15 ~ 20min, be then at the uniform velocity cooled to room temperature, with the centrifugal 15 ~ 20min of the rotating speed of 8000 ~ 10000r/min, get supernatant.
In step (3), the condition of micro-filtration was the filter membrane of 0.22 ~ 0.65 μm.
In step (3), micro-filtrate is added in the ultra-filtration membrane 30KD centrifuge tube of 15mL dress, be placed in the centrifugal 20-40min of 5000-7000rpm in whizzer, open super filter tube lid, with liquid-transfering gun by ultra-filtration membrane filtered solution and the sucking-off separately of non-filtered solution, the centrifuge tube being 10KD with 3KD through ultra-filtration membrane again through the filtrate of ultra-filtration membrane is separated, and obtains the polypeptide liquid that each group of molecular weight is different, and measures the inhibiting rate of each component to alpha-glucosidase.
In step (3), cryodesiccated temperature is-50 DEG C.
Present invention further proposes the thick polypeptide of the HUIDOUBA utilizing above-mentioned preparation method to prepare for the preparation of the application on the medicine of hypoglycemic.
Beneficial effect: compared with prior art, the present invention utilizes enzyme to be hydrolyzed HUIDOUBA crude protein, does not only avoid the toxic side effect that chemical reagent brings, and can improve the yield of HUIDOUBA polypeptide.By preferably suitable proteolytic enzyme and enzymatic hydrolysis condition, cost of the present invention is low, technique is simple, the cycle is short, controllability is strong, productive rate is high, can realize large-scale industrialized production.
Accompanying drawing explanation
The content of peptides of each molecular weight of Fig. 1 Ultra filtration membrane and inhibiting rate.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, processing condition and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
The extraction of embodiment 1 HUIDOUBA crude protein.
What accurately take that 1g pulverized goes native HUIDOUBA, HUIDOUBA crude protein is extracted under the condition of pH 7.5, ultrasonic power 800W, time 50min, liquid ratio 50: 1 (mL/g) and temperature 60 C, at the centrifugal 20min of 3500r/min after question response terminates, repeat experiment 3 times, the average content obtaining HUIDOUBA crude protein is 48.15%.
The extraction of embodiment 2 HUIDOUBA crude protein.
What accurately take that 1g pulverized goes native HUIDOUBA, HUIDOUBA crude protein is extracted under the condition of pH 7, ultrasonic power 700W, time 45min, liquid ratio 55: 1 (mL/g) and temperature 50 C, at the centrifugal 15min of 4000r/min after question response terminates, repeat experiment 3 times, the average content obtaining HUIDOUBA crude protein is 49.61%.
The extraction of embodiment 3 HUIDOUBA crude protein.
What accurately take that 1g pulverized goes native HUIDOUBA, HUIDOUBA crude protein is extracted under the condition of pH 8, ultrasonic power 900W, time 55min, liquid ratio 60: 1 (mL/g) and temperature 55 DEG C, at the centrifugal 10min of 5000r/min after question response terminates, repeat experiment 3 times, the average content obtaining HUIDOUBA crude protein is 49.75%.
The extraction of embodiment 4 HUIDOUBA crude protein.
What accurately take that 1g pulverized goes native HUIDOUBA, HUIDOUBA crude protein is extracted under the condition of pH 6.5, ultrasonic power 750W, time 55min, liquid ratio 45: 1 (mL/g) and temperature 55 DEG C, at the centrifugal 15min of 4000r/min after question response terminates, repeat experiment 3 times, the average content obtaining HUIDOUBA crude protein is 51.69%.
The extraction of embodiment 5 HUIDOUBA crude protein.
What accurately take that 1g pulverized goes native HUIDOUBA, HUIDOUBA crude protein is extracted under the condition of pH 7, ultrasonic power 850W, time 60min, liquid ratio 50: 1 (mL/g) and temperature 55 DEG C, at the centrifugal 15min of 4500r/min after question response terminates, repeat experiment 3 times, the average content obtaining HUIDOUBA crude protein is 52.42%.
The extraction of embodiment 6 HUIDOUBA crude protein.
Be optimized process with response phase method to above-mentioned HUIDOUBA protein extraction condition, under the condition of power 800W, time 53min, temperature 58 DEG C, pH 7.5 and liquid ratio 54: 1mL/g, the extraction yield of HUIDOUBA albumen is 52.66%.
Wherein the extraction yield calculation formula of HUIDOUBA albumen is as follows:
In formula: η is HUIDOUBA protein extracting ratio/%; C is testing protein quality concentration/(mg/mL) that establishing criteria opisometer calculates; V is extracting liquid volume/mL; M is the quality/g of sample; Y is HUIDOUBA total protein content/%.
The preparation of the thick polypeptide of embodiment 7 HUIDOUBA.
HUIDOUBA crude protein powder prepared by Example 1, is mixed with deionized water the solution that concentration is 5wt%, by the condition of table 1, under the theoretical optimal conditions of various enzyme, carries out enzyme digestion reaction, according to polypeptide yield, chooses optimum proteolytic enzyme.
The selection of table 1 proteolytic enzyme
Wherein, the calculation formula of polypeptide yield is as follows:
In formula: η is the yield/% of HUIDOUBA polypeptide; Peptide concentration/(mg/mL) of C for obtaining according to typical curve; V is enzymolysis solution volume/mL; M is the quality/mg of HUIDOUBA crude protein sample; Y is the content/% of HUIDOUBA crude protein.
Experimental result is as shown in table 2.
Polypeptide yield after the enzymolysis of table 2 proteolytic enzyme
Through the comparison to Sumizyme MP, neutral protease, trypsinase, papoid, stomach en-, compound protease and flavor protease, optimum proteolytic enzyme is selected to be compound protease (purchased from Yuan Ye bio tech ltd, Shanghai, Rate activity is 120U/mg).
The preparation of the thick polypeptide of embodiment 8 HUIDOUBA.(data of amendment are passable, just by this.Solid-liquid ratio is also right.)
Preparing concentration of substrate with the phosphoric acid buffer that pH is 6.5 is 5wt% HUIDOUBA protein solution 10mL, add compound protease, enzyme concentration (E/S) is 6000U/g, enzymolysis 3h under temperature 45 C, after enzyme digestion reaction terminates, carries out deactivation 15min by enzymolysis solution boiling water bath, then centrifugal 20min under the rotating speed of 10000r/min, get its centrifugal after supernatant liquor, with this understanding, the polypeptide yield of the HUIDOUBA crude protein that enzymolysis obtains is 67.58%.
The preparation of the thick polypeptide of embodiment 9 HUIDOUBA.
Preparing concentration of substrate with the phosphoric acid buffer that pH is 7 is 6wt% HUIDOUBA protein solution 10mL, add compound protease, enzyme concentration (E/S) is 5000U/g, enzymolysis 2.5h at temperature 40 DEG C, after enzyme digestion reaction terminates, carries out deactivation 20min by enzymolysis solution boiling water bath, then centrifugal 20min under the rotating speed of 8000r/min, get its centrifugal after supernatant liquor, with this understanding, the polypeptide yield of the HUIDOUBA crude protein that enzymolysis obtains is 69.64%.
The preparation of the thick polypeptide of embodiment 10 HUIDOUBA.
Preparing concentration of substrate with the phosphoric acid buffer that pH is 7.5 is 7wt% HUIDOUBA protein solution 10mL, add compound protease, enzyme concentration (E/S) is 5500U/g, enzymolysis 4h under temperature 50 C, after enzyme digestion reaction terminates, carries out deactivation 15min by enzymolysis solution boiling water bath, then centrifugal 15min under the rotating speed of 9000r/min, get its centrifugal after supernatant liquor, with this understanding, the polypeptide yield of the HUIDOUBA crude protein that enzymolysis obtains is 72.47%.
The preparation of the thick polypeptide of embodiment 11 HUIDOUBA.
Preparing concentration of substrate with the phosphoric acid buffer that pH is 6.5 is 6wt% HUIDOUBA protein solution 10mL, add compound protease, enzyme concentration (E/S) is 6000U/g, enzymolysis 4h at temperature 40 DEG C, after enzyme digestion reaction terminates, carries out deactivation 20min by enzymolysis solution boiling water bath, then centrifugal 20min under the rotating speed of 10000r/min, get its centrifugal after supernatant liquor, with this understanding, the polypeptide yield of the HUIDOUBA crude protein that enzymolysis obtains is 75.91%.
The preparation of the thick polypeptide of embodiment 12 HUIDOUBA.
Preparing concentration of substrate with the phosphoric acid buffer that pH is 7 is 7wt% HUIDOUBA protein solution 10mL, add compound protease, enzyme concentration (E/S) is 4500U/g, enzymolysis 3h under temperature 50 C, after enzyme digestion reaction terminates, carries out deactivation 20min by enzymolysis solution boiling water bath, then centrifugal 15min under the rotating speed of 8000r/min, get its centrifugal after supernatant liquor, with this understanding, the polypeptide yield of the HUIDOUBA crude protein that enzymolysis obtains is 75.73%.
The preparation of the thick polypeptide of embodiment 13 HUIDOUBA.
With response phase method, process is optimized to above-mentioned HUIDOUBA proteolysis condition, pH be 6.7 phosphoric acid buffer preparation concentration of substrate be 6wt% HUIDOUBA protein solution 10mL, add compound protease, enzyme concentration (E/S) is 5400U/g, enzymolysis 3.5h at temperature 40 DEG C, after enzyme digestion reaction terminates, enzymolysis solution boiling water bath is carried out deactivation 15min, then low-temperature centrifugation 10min under the rotating speed of 10000r/min, get its centrifugal after supernatant liquor, with this understanding, the polypeptide yield of enzymolysis HUIDOUBA albumen is 76.72%.
The purifying of the thick polypeptide of embodiment 14 HUIDOUBA and application.
The polypeptide solution that embodiment 13 obtains is crossed the filter membrane of 0.45 μm, micro-filtrate is added in the ultra-filtration membrane 30KD centrifuge tube of 15mL dress, be placed in the centrifugal 30min of 6000rpm in whizzer, open super filter tube lid, with liquid-transfering gun by ultra-filtration membrane filtered solution and the sucking-off separately of non-filtered solution, the centrifuge tube being 10KD with 3KD through ultra-filtration membrane again through the filtrate of ultra-filtration membrane is separated, and obtains the polypeptide liquid that each group of molecular weight is different.Each component polypeptide liquid obtains HUIDOUBA polypeptide powder after concentrated, freezing ,-50 DEG C of dryings, and the thick polypeptide powder deionized water of HUIDOUBA getting each component is mixed with the polypeptide solution of 30mg/mL respectively.
The thick polypeptide of HUIDOUBA is to the mensuration of the suppression of alpha-glucosidase:
1000 μ L pH are the phosphate buffer solution (0.1M) of 6.8, the thick polypeptide sample of 200 μ L, 50 μ L reductive glutathiones (1mg/mL), 50 μ L alpha-glucosidases (2.5U/mL), mixing, 10min is reacted under 37 DEG C of conditions, add 100 μ L pNPG (0.5mg/mL), mixing, 37 DEG C of Water Under bath 15min, add 1000 μ L sodium carbonate solution (0.1mol/L) termination reactions, measure solution absorbance value at 405nm place, be denoted as A
sample.Sample is replaced, the same A of other conditions with phosphoric acid buffer
sample, absorbance is denoted as A
contrast; Enzyme is replaced, the same A of other conditions with phosphoric acid buffer
sample, be denoted as A blank.The inhibiting rate calculation formula of the thick polypeptide sample of HUIDOUBA to alpha-glucosidase is as follows:
Experimental result as shown in Figure 1.Can be known by experimental result, polypeptide molecular weight is the highest in this scope of 3 ~ 10KD, and proportion is 32.47%, and is also the highest to alpha-glucosaccharase enzyme inhibition rate, is 82.61%.
Claims (9)
1. a preparation method for the thick polypeptide of HUIDOUBA, is characterized in that, comprise the steps:
(1) extraction of HUIDOUBA crude protein: the HUIDOUBA raw material after pulverizing is joined in phosphoric acid buffer and soaks, extract under Ultrasonic Conditions, centrifugal, get supernatant liquor and lyophilize, obtain HUIDOUBA crude protein powder;
(2) enzymolysis: HUIDOUBA crude protein powder step (1) obtained is mixed with the aqueous solution, adds proteolytic enzyme and carries out enzyme digestion reaction, obtain the enzymolysis solution of HUIDOUBA crude protein to it;
(3) purifying: the enzymolysis solution that step (2) obtains to go out enzyme, centrifugal, micro-filtration through boiling water bath, the filtrate obtained carries out centrifugal treating with the ultra-filtration centrifuge tube of different molecular weight respectively, the polypeptide liquid of the different molecular weight obtained is carried out concentrated and lyophilize, obtain the thick polypeptide of HUIDOUBA of different molecular weight.
2. the preparation method of the thick polypeptide of HUIDOUBA according to claim 1, it is characterized in that, in step (1), the condition of ultrasonic extraction is: in ultrasonic power 700-900W, ultrasonic time 45 ~ 60min, temperature 50-60 DEG C, pH 6-8 and liquid ratio (45 ~ 60): extract HUIDOUBA albumen under the condition of 1mL/g, at the centrifugal 10 ~ 20min of 3000-5000r/min after question response terminates, supernatant concentration lyophilize, cryodesiccated temperature is-50 DEG C.
3. the preparation method of the thick polypeptide of HUIDOUBA according to claim 1, it is characterized in that, in step (2), described proteolytic enzyme is any one in Sumizyme MP, neutral protease, trypsinase, papoid, compound protease and flavor protease.
4. the preparation method of the thick polypeptide of HUIDOUBA according to claim 1, it is characterized in that, in step (2), the consumption of described proteolytic enzyme is 4000 ~ 6000U/g, HUIDOUBA crude protein concentration is 5 ~ 7wt%, the temperature of enzymolysis is 30 ~ 50 DEG C, and the time of enzymolysis is 2 ~ 4h, and the pH of enzymolysis is 6 ~ 8.
5. the preparation method of the thick polypeptide of HUIDOUBA according to claim 1, it is characterized in that, in step (3), the go out condition of enzyme of boiling water bath is: enzymolysis solution is placed in boiling water bath and keeps 15 ~ 20min, then at the uniform velocity room temperature is cooled to, with the centrifugal 15 ~ 20min of the rotating speed of 8000 ~ 10000r/min, get supernatant.
6. the preparation method of the thick polypeptide of HUIDOUBA according to claim 1, is characterized in that, in step (3), the condition of micro-filtration was the filter membrane of 0.22-0.65 μm.
7. the preparation method of the thick polypeptide of HUIDOUBA according to claim 1, it is characterized in that, in step (3), micro-filtrate is added in the ultra-filtration membrane 30KD centrifuge tube of 15mL dress, be placed in the centrifugal 20-40min of 5000-7000rpm in whizzer, open super filter tube lid, with liquid-transfering gun by ultra-filtration membrane filtered solution and the sucking-off separately of non-filtered solution, the centrifuge tube being 10KD with 3KD through ultra-filtration membrane again through the filtrate of ultra-filtration membrane is separated, the polypeptide liquid that molecular weight is different respectively.
8. the preparation method of the thick polypeptide of HUIDOUBA according to claim 1, is characterized in that, in step (3), cryodesiccated temperature is-50 DEG C.
9. the thick polypeptide of HUIDOUBA prepared of preparation method according to claim 1 is for the preparation of the application on the medicine of hypoglycemic.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115336755A (en) * | 2022-08-17 | 2022-11-15 | 北京纳百恩食品有限公司 | Fibrinolysis-promoting active substance and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583087A (en) * | 2004-05-22 | 2005-02-23 | 刘贤英 | Chinese health-care medicine for reducing blood sugar and preventing from diabete complication and its preparation |
| CN1687444A (en) * | 2005-03-23 | 2005-10-26 | 江苏大学 | Method for preparing peptide of decrease blood pressure in laver by using enzyme-membrane coupling technique and application thereof |
| CN102154423A (en) * | 2011-01-12 | 2011-08-17 | 淮海工学院 | Method for preparing active peptide of laver |
| CN102224879A (en) * | 2011-06-09 | 2011-10-26 | 申健 | Preparation method and application of champignon polypeptide |
-
2014
- 2014-12-16 CN CN201410782005.4A patent/CN104450847A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583087A (en) * | 2004-05-22 | 2005-02-23 | 刘贤英 | Chinese health-care medicine for reducing blood sugar and preventing from diabete complication and its preparation |
| CN1687444A (en) * | 2005-03-23 | 2005-10-26 | 江苏大学 | Method for preparing peptide of decrease blood pressure in laver by using enzyme-membrane coupling technique and application thereof |
| CN102154423A (en) * | 2011-01-12 | 2011-08-17 | 淮海工学院 | Method for preparing active peptide of laver |
| CN102224879A (en) * | 2011-06-09 | 2011-10-26 | 申健 | Preparation method and application of champignon polypeptide |
Non-Patent Citations (2)
| Title |
|---|
| 伍艳等: "中药灰兜巴有效化学成分分析及降糖实验研究", 《中国畜牧兽医学会兽医药理毒理学分会第十次研讨会》 * |
| 伍艳等: "灰兜巴活性成分治疗Ⅱ型糖尿病效果研究", 《JOURNAL OF CHINESE MEDICINAL MATERIALS》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115336755A (en) * | 2022-08-17 | 2022-11-15 | 北京纳百恩食品有限公司 | Fibrinolysis-promoting active substance and preparation method and application thereof |
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Application publication date: 20150325 |