CN104450582B - Lactobacillus plantarum and feed preblending agent containing the Lactobacillus plantarum and preparation method thereof - Google Patents
Lactobacillus plantarum and feed preblending agent containing the Lactobacillus plantarum and preparation method thereof Download PDFInfo
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Abstract
Feed preblending agent the invention discloses a kind of Lactobacillus plantarum and containing the Lactobacillus plantarum and preparation method thereof, the deposit number of the Lactobacillus plantarum Lactobacillus plantarum is：CGMCC No.9642.The complex culture medium that the feed preblending agent that the present invention is provided is composited using whey powder, sucrose, purslane extract, Rhizoma Smilacis Glabrae extract, Astragalus Root P.E and Herba Euphorbiae Humifusae extract carries out anaerobic fermentation, feed preblending agent is made as culture medium.The feed preblending agent contains abundant active polysaccharide, flavones, viable bacteria body and its metabolite, as the micro-ecological feed pre-mixing agent of prawn culturing, viable bacteria body and its metabolism lactic acid can suppress enteropathogenic bacteria growing, active polysaccharide, flavones, the metabolite of viable bacteria body are easier to be absorbed by shrimp, can effectively play viable bacteria body and active polysaccharide, the multiple-effect of metabolite.
Description
Technical field
It is more particularly to a kind of to be used for prophylactic treatment enteron aisle the present invention relates to microbe application and feed preparation manufacture field
The Lactobacillus plantarum of disease and immunological diseases and the feed preblending agent containing the Lactobacillus plantarum and preparation method thereof.
Background technology
1,500,000 mu of the total cultured area of Penaeus Vannmei fresh water, seawater of China in 2005, to 2010 i.e. more than 3,000,000
Mu, ten thousand tons of annual production 90-100.Started the specialty that culture of Penaeus vannamei is no longer southern four province to 2012；North extend
Developed rapidly to Jiangsu and Zhejiang Provinces, Shandong, Coast of Hebei Province area to more than 150 ten thousand mu.Since 2011, the disease of culture of Penaeus vannamei is dead
Rate more and more higher is died, rate is formed below 20% in most of areas；To the first half of the year in 2011 in provinces such as Hainan, Guangdong and Guangxi
Yield more than 80% is have dropped than the same period, bring massive losses to white shrimp aquaculture.It is primarily due to water body life
State deteriorate trigger enteritis, it is white just, the empty stomach of jejunum, the enteron aisle disease such as the apocleisis trip pool it is more and more.Because the immunocyte of prawn is produced
Enteron aisle is born in, therefore enteron aisle disease has a strong impact on immunocyte vigor, causes the death rate of prawn very high.
The content of the invention
In view of this, it is an object of the invention to propose that a lactobacillus plantarum and feed containing the Lactobacillus plantarum are pre-
Mixture and preparation method thereof, to reduce the death rate of prawn.
The Lactobacillus plantarum (Lactobacillus plantarum) provided based on above-mentioned purpose, the present invention, the bacterial strain
China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved in, preservation place is Chaoyang District, Beijing City North Star west
No. 3 Institute of Microorganism, Academia Sinica of institute of road 1, the numbering that preservation is registered on the books is CGMCC No.9642, and preservation date is
On September 5th, 2014.
The present invention also provides the Lactobacillus plantarum Lactobacillus plantarum in feed preblending agent is prepared
Using.
The present invention also provides a kind of preparation method of feed preblending agent, and the feed preblending agent preparation method includes：With institute
Lactobacillus plantarum Lactobacillus plantarum are stated for fermented bacterium, with whey powder, sucrose, purslane extract, soil Fu
The complex culture medium that Siberian cocklebur extract, Astragalus Root P.E and Herba Euphorbiae Humifusae extract are composited carries out anaerobic fermentation as culture medium,
Viable bacteria content >=3 × 10 are made8CFU/ml feed preblending agent.
It is preferred that the feed preblending agent preparation method comprises the following steps：
1) by purslane, smilax, the Radix Astragali and wolf's milk according to 1~2:1~2:1~2:1~4 weight ratio puts into water
In, obtain extract solution through boiling；
2) whey powder and sucrose are added in the extract solution, adjust the pH of extract solution to 6.4~7.5, sterilized, drop
Wen Hou, accesses under the Lactobacillus plantarum Lactobacillus plantarum strains, anaerobic condition and ferments, that is, obtains described
Feed preblending agent containing Lactobacillus plantarum Lactobacillus plantarum.
Preferably, after inoculated plant lactobacillus Lactobacillus plantarum, the temperature of the anaerobic fermentation is 30
DEG C~35 DEG C, the anaerobic fermentation time is 26~35 hours, that is, obtains Lactobacillus plantarum Lactobacillus plantarum effective
Viable bacteria content is 3 × 108~8 × 109CFU/ml feed preblending agent.
Alternatively, the inoculum concentration of the Lactobacillus plantarum Lactobacillus plantarum is 5%~10%.
The present invention also provides a kind of feed preblending agent, and the feed preblending agent is according to the preparation method of above-mentioned feed preblending agent
Prepare.
It is preferred that the effective viable bacteria contents of Lactobacillus plantarum Lactobacillus plantarum in the feed preblending agent
For 3 × 108~8 × 109CFU/ml。
The present invention also provides the application of above-mentioned feed preblending agent, by the feed preblending agent according to feeding quality 2%~
5% is added in feed, and is premixed before feeding.
It is preferred that making feed after the feed preblending agent and feed, premix being premixed in sealing container within 1~2 hour in advance
Pre-mixing agent anaerobism under the conditions of 20 DEG C~30 DEG C is activated.
From the above it can be seen that the feed preblending agent that provides of the present invention with whey powder, sucrose, purslane extract,
The complex culture medium that Rhizoma Smilacis Glabrae extract, Astragalus Root P.E and Herba Euphorbiae Humifusae extract are composited carries out anaerobism as culture medium
Fermentation, is made viable bacteria content >=3 × 108CFU/ml feed preblending agent.The feed preblending agent contains abundant active polysaccharide, Huang
Ketone, viable bacteria body and its metabolite, as the micro-ecological feed pre-mixing agent of prawn culturing, viable bacteria body and its metabolism lactic acid can
Suppress enteropathogenic bacteria growing, active polysaccharide, flavones, viable bacteria body metabolite (amino acid, vitamin, trace element etc.) more
Easily absorbed by shrimp, can effectively play viable bacteria body and active polysaccharide, the multiple-effect of metabolite.
Embodiment
For the object, technical solutions and advantages of the present invention are more clearly understood, below in conjunction with specific embodiment, to this hair
It is bright to be further described.
The Lactobacillus plantarum Lactobacillus plantarum of embodiment 1 separation and identification
The Lactobacillus plantarum BW2013 bacterial strains were deposited in Chinese microorganism strain preservation management committee on the 5th in September in 2014
Member understands common micro-organisms center, and deposit number is：CGMCC No.9642, depositary institution address：BeiChen West Road, Chaoyang District, BeiJing City
No. 1 institute 3, Institute of Microorganism, Academia Sinica, postcode：100101.
This strain separating is in Beijing Suburb vegetable cultivation base, and using MRS culture mediums, spread plate is by aubergine pigment point
From and obtain, 20~38 DEG C of its growth temperature, 35 DEG C of optimum growth temperature.Suitable growth pH4.0~7.5, optimum growth
PH6.5, culture presevation uses MRS culture mediums.The newborn bar of plant is accredited as through Environmental Biotechnology key lab of the Chinese Academy of Sciences
Bacterium (Lactobacillus plantarum).
The preparation method of feed preblending agent of the embodiment 2 containing Lactobacillus plantarum Lactobacillus plantarum
Using the Lactobacillus plantarum Lactobacillus plantarum as fermented bacterium, with whey powder, sucrose, dent
The complex culture medium that amaranth extract, Rhizoma Smilacis Glabrae extract, Astragalus Root P.E and Herba Euphorbiae Humifusae extract are composited as culture medium,
Anaerobic fermentation is carried out, viable bacteria content >=3 × 10 are made8CFU/ml feed preblending agent.
It is used as one embodiment of the present of invention, the feeding containing Lactobacillus plantarum Lactobacillus plantarum
Expect that the preparation method of pre-mixing agent is following (by taking 1000L as an example)：
(1) purslane 10kg, smilax 10kg, Radix Astragali 10kg and wolf's milk 20kg are delivered and arrives 1000L Chinese medicine extracting tanks
Interior, add water 600L for the first time, after immersion 2h, while being stirred with 50~60 revs/min of speed, one side heat temperature raising is warming up to 100
DEG C stirring 1h；Then 40 DEG C~50 DEG C are cooled to, filtrate, about 400L are collected in filtering.350L is added water for the second time；It is warming up to 100
DEG C stirring 40min；40 DEG C~50 DEG C are cooled to, filtered fluid, about 300L are collected in filtering.Third time adds water 350L；It is warming up to
100 DEG C of stirring 40min；It is cooled to 40 DEG C~50 DEG C, filtered fluid is collected in filtering, about 300L, collects Chinese medicine extract about altogether three times
1000L。
(2) potassium dihydrogen phosphate 600g, dipotassium hydrogen phosphate 300g, calcium chloride 30g, ammonium sulfate 300g, magnesium sulfate 120g, sugarcane are taken
Sugared 10kg and whey powder 15kg is added in above-mentioned 1000L Chinese medicine extracts, is stirred.PH value of solution is adjusted with 12N sodium hydroxides
To 6.8~7.5；Then sterilize 30min in 121 DEG C, then is cooled to 30 DEG C~35 DEG C inoculation Lactobacillus plantarums
Lactobacillus plantarum, inoculum concentration is 7.5%~10%, 30 DEG C~35 DEG C of constant temperature, after 28~30h of anaerobic fermentation,
It is 3 × 10 to obtain Lactobacillus plantarum viable bacteria content8~7.5 × 109CFU/ml zymotic fluid, i.e., it is described to contain Lactobacillus plantarum
Lactobacillus plantarum feed preblending agent.Also contain polysaccharide and flavones in the zymotic fluid.
Wherein, the purslane, smilax, the Radix Astragali and wolf's milk are to be done without rotten, free from insect pests, without the high-quality gone mouldy
Product.
It is described to contain Lactobacillus plantarum Lactobacillus plantarum's as another embodiment of the present invention
The preparation method of feed preblending agent is following (by taking 1000L as an example)：
(1) purslane 12kg, smilax 13kg, Radix Astragali 9kg and wolf's milk 18kg are delivered and arrives 1000L Chinese medicine extracting tanks
Interior, add water 600L for the first time, after immersion 2h, while being stirred with 50~60 revs/min of speed, one side heat temperature raising is warming up to 100
DEG C stirring 1h；Then 40 DEG C~50 DEG C are cooled to, filtrate, about 400L are collected in filtering.350L is added water for the second time；It is warming up to 100
DEG C stirring 40min；40 DEG C~50 DEG C are cooled to, filtered fluid, about 300L are collected in filtering.Third time adds water 350L；It is warming up to
100 DEG C of stirring 40min；It is cooled to 40 DEG C~50 DEG C, filtered fluid is collected in filtering, about 300L, collects Chinese medicine extract about altogether three times
1000L。
(2) potassium dihydrogen phosphate 650g, dipotassium hydrogen phosphate 310g, calcium chloride 25g, ammonium sulfate 300g, magnesium sulfate 110g, sugarcane are taken
Sugared 9.5kg and whey powder 18kg is added in above-mentioned 1000L Chinese medicine extracts, is stirred.PH value of solution is adjusted with 10N sodium hydroxides
To 6.4~7.2；Then sterilize 40min in 118 DEG C, then is cooled to 30 DEG C~35 DEG C inoculation Lactobacillus plantarums
Lactobacillus plantarum, inoculum concentration is 5%~8%, 30 DEG C~35 DEG C of constant temperature, after 26~30h of anaerobic fermentation, is obtained
It is 8 × 10 to Lactobacillus plantarum viable bacteria content8~8 × 109CFU/ml zymotic fluid, i.e., it is described to contain Lactobacillus plantarum
Lactobacillus plantarum feed preblending agent.Also contain polysaccharide and flavones in the zymotic fluid.
It is described to contain Lactobacillus plantarum Lactobacillus plantarum's as another embodiment of the present invention
The preparation method of feed preblending agent is following (by taking 1000L as an example)：
(1) purslane 15kg, smilax 8kg, Radix Astragali 13.5kg and wolf's milk 32kg are delivered and arrives 1000L Chinese medicine extracting tanks
Interior, add water 600L for the first time, after immersion 2h, while being stirred with 50~60 revs/min of speed, one side heat temperature raising is warming up to 100
DEG C stirring 1h；Then 40 DEG C~50 DEG C are cooled to, filtrate, about 400L are collected in filtering.350L is added water for the second time；It is warming up to 100
DEG C stirring 40min；40 DEG C~50 DEG C are cooled to, filtered fluid, about 300L are collected in filtering.Third time adds water 350L；It is warming up to
100 DEG C of stirring 40min；It is cooled to 40 DEG C~50 DEG C, filtered fluid is collected in filtering, about 300L, collects Chinese medicine extract about altogether three times
1000L。
(2) potassium dihydrogen phosphate 600g, dipotassium hydrogen phosphate 300g, calcium chloride 30g, ammonium sulfate 300g, magnesium sulfate 120g, sugarcane are taken
Sugared 10kg and whey powder 15kg is added in above-mentioned 1000L Chinese medicine extracts, is stirred.PH value of solution is adjusted with 12N sodium hydroxides
To 6.8~7.15；Then sterilize 30min in 125 DEG C, then is cooled to 30 DEG C~35 DEG C inoculation Lactobacillus plantarums
Lactobacillus plantarum, inoculum concentration is 5.8%~9.6%, 30 DEG C~35 DEG C of constant temperature, 27.6~33h of anaerobic fermentation
Afterwards, it is 3 × 10 to obtain Lactobacillus plantarum viable bacteria content8~8 × 109CFU/ml zymotic fluid, i.e., it is described to contain Lactobacillus plantarum
Lactobacillus plantarum feed preblending agent.Also contain polysaccharide and flavones in the zymotic fluid.
The purposes of feed preblending agent described in embodiment 3
The purposes of the feed preblending agent includes：
(1) feed preblending agent is added in feed according to the 2%~5% of feeding quality, and premixed before feeding.
It can be fed according to shrimp seedling size before bait throwing in material using the feed preblending agent premix of Different adding amount into prawn feed
Support.
(2) shift to an earlier date 1~2 hour and tighten polybag after premixing feedstuff pre-mixing agent and feed, premix in clean polybag
Mouth make feed preblending agent anaerobism activate, and make in feed preblending agent active principle (such as active polysaccharide, flavones, viable bacteria body with
And its metabolite etc.) fully penetrate into feed.According to cultivation needs can add daily the feed preblending agent 1 time or
Repeatedly.
(3) when feeding, the feed that is even and being activated through anaerobism that is mixed will be mixed and directly delivered onto the material platform on the shrimp pool.
As one embodiment of the present of invention, the feed preblending agent is added to according to the 2%~3.5% of feeding quality
In feed, and make feed after the feed preblending agent and feed, premix were premixed in sealing container at 1~1.5 hour before feeding
Pre-mixing agent anaerobism under the conditions of 20 DEG C~27 DEG C is activated.When feeding, the feed that is even and being activated through anaerobism that is mixed will be mixed and directly delivered
Onto the material platform on the shrimp pool.
As one embodiment of the present of invention, the feed preblending agent is added to according to the 3.3%~5% of feeding quality
In feed, and make feed after the feed preblending agent and feed, premix were premixed in sealing container at 1.4~2 hours before feeding
Pre-mixing agent anaerobism under the conditions of 24 DEG C~30 DEG C is activated.When feeding, the feed that is even and being activated through anaerobism that is mixed will be mixed and directly delivered
Onto the material platform on the shrimp pool.
The nursing experiment of feed preblending agent described in embodiment 4
(1) Penaeus Vannmei feeds experiment in 99 days
Place：Guangdong Province Fanyu District sea-gull island Chen Gui prawns, the intensive cultures of Low-salinity Tu Tang half.
Experimental method：Using same batch shrimp seedling, deliver on the same day.From honest feed, setting experiment 1 is by 1.5%, experiment 2
Mixed by 2.0%, experiment 3 by 2.5% and feed the feed premix containing Lactobacillus plantarum Lactobacillus plantarum
Agent, mixes feed twice daily sooner or later.Control group premixes the warm water of isodose.Daily dead shrimp meter is collected from the dirty pipe sleeve net in row bottom
Number.
Breeding water body average salinity 28.2 DEG C of times of 1.0/1000 average temperature：2 days-July 5 April in 2013
| Area (mu) | Throw seedling (tail/mu) | 40 days average substances | 90 days substances | The death rate | Average yield per mu | |
| Experiment 1 | 3.3 | 50000 | 5.80g | 12.46g | 18.65% | 505kg |
| Experiment 2 | 3.1 | 50000 | 6.32g | 15.06g | 17.00% | 625kg |
| Experiment 3 | 2.4 | 50000 | 6.12g | 14.89g | 18.87% | 605kg |
| Control 1 | 3.0 | 50000 | 4.21g | 10.56g | 54.90% | 290kg |
| Control 2 | 3.8 | 50000 | 4.37g | 10.61g | 48.18% | 275kg |
(2) Penaeus Vannmei feeds experiment in 99 days
Place：The gentle shrimp in Zhanjiang, Guangdong Province East Sea Island, high-order culture pond.
Experimental method：Using same batch Zhengda Company shrimp seedling, from Zhengda Company's feed.Setting experiment 1 is by 1.5%, reality
Test 2 and mix hello the feed premix containing Lactobacillus plantarum Lactobacillus plantarum by 3% by 2.0%, experiment 3
Agent, mixes feed twice daily sooner or later.Control group mixes the warm water for feeding isodose.Daily dead shrimp meter is collected from the dirty pipe sleeve net in row bottom
Number.
Breeding water body average salinity 29.6 DEG C of times of 14/1000 average temperature：August in 2013 16 days-November 17
(3) Penaeus Vannmei feeds experiment in 100 days
Place：The high-order winter canopy culture pond of Hainan Province Qionghai mayor slope town symbol poplar Low-salinity.
Experimental method：Using the honest shrimp seedling of same batch, Guangdong Yue Hai feeds are selected, setting experiment 1 is pressed by 1.5%, experiment 2
2.0%th, experiment 3 presses 2.5%, and experiment 4 is mixed by 3% feeds described containing Lactobacillus plantarum Lactobacillus plantarum's
Feed preblending agent, mixes feed twice daily sooner or later.3 control groups mix the warm water for feeding isodose.Daily from the dirty pipe sleeve net in row bottom
Dead shrimp is collected to count.
20 days January -2014 years on the 19th October in 2013 of 28.5 DEG C of average 2/1000 average temperature of salinity of breeding water body
The feed preblending agent that the present invention is provided is carried with whey powder, sucrose, purslane extract, Rhizoma Smilacis Glabrae extract, the Radix Astragali
Take the complex culture medium that thing and Herba Euphorbiae Humifusae extract are composited as culture medium, carry out anaerobic fermentation, viable bacteria content >=3 are made
×108CFU/ml feed preblending agent.The feed preblending agent contains abundant active polysaccharide, flavones, viable bacteria body and its metabolism production
Thing, as the micro-ecological feed pre-mixing agent of prawn culturing, viable bacteria body and its metabolism lactic acid can suppress enteropathogenic bacteria growing,
Active polysaccharide, flavones, the metabolite (amino acid, vitamin, trace element etc.) of viable bacteria body are easier to be absorbed by shrimp, Ke Yiyou
Effect plays viable bacteria body and active polysaccharide, the multiple-effect of metabolite.
Especially to having good prevention effect to Penaeus Vannmei enteron aisle disease, the empty stomach of its jejunum, intestines are can obviously reduce
Scorching, the white enteron aisle disease such as just；Greatly improve cultivation survival rate.It is white as South America that the method provided by the present invention obtains zymotic fluid
The feed preblending agent development and application of prawn culturing have wide market prospects.
Those of ordinary skills in the art should understand that：The specific embodiment of the present invention is the foregoing is only, and
The limitation present invention is not used in, within the spirit and principles of the invention, any modification, equivalent substitution and improvements done etc.,
It should be included within protection scope of the present invention.
Claims (10)
1. a kind of Lactobacillus plantarum (Lactobacillus plantarum), it is characterised in that it is micro- that the bacterial strain is preserved in China
Biological inoculum preservation administration committee common micro-organisms center, preservation place is in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Institute of microbiology of the academy of sciences of state, the numbering that preservation is registered on the books is CGMCC No.9642, and preservation date is September 5 in 2014
Day.
2. application of the Lactobacillus plantarum according to claim 1 in feed preblending agent is prepared.
3. a kind of preparation method of feed preblending agent, it is characterised in that including：Using the Lactobacillus plantarum as fermented bacterium, with
It is compound that whey powder, sucrose, purslane extract, Rhizoma Smilacis Glabrae extract, Astragalus Root P.E and Herba Euphorbiae Humifusae extract are composited
Nutrient solution carries out anaerobic fermentation, viable bacteria content >=3 × 10 is made as culture medium8CFU/ml feed preblending agent.
4. the preparation method of feed preblending agent according to claim 3, it is characterised in that comprise the following steps：
1) by purslane, smilax, the Radix Astragali and wolf's milk according to 1~2:1~2:1~2:1~4 weight ratio is put into water,
Extract solution is obtained through boiling；
2) whey powder and sucrose are added in the extract solution, adjust the pH of extract solution to 6.4~7.5, sterilized, cooling
Afterwards, access under the Lactobacillus plantarum strain, anaerobic condition and ferment, that is, obtain the feed premix containing Lactobacillus plantarum
Agent.
5. the preparation method of feed preblending agent according to claim 4, it is characterised in that after inoculated plant lactobacillus, institute
The temperature for stating anaerobic fermentation is 30 DEG C~35 DEG C, and the anaerobic fermentation time is 26~35 hours, that is, obtains Lactobacillus plantarum and effectively live
Bacterial content is 3 × 108~8 × 109CFU/ml feed preblending agent.
6. the preparation method of feed preblending agent according to claim 4, it is characterised in that the inoculation of the Lactobacillus plantarum
Measure as 5%~10%.
7. a kind of feed preblending agent, it is characterised in that the feed preblending agent is according to any one in claim 3~6
The preparation method of feed preblending agent prepare.
8. feed preblending agent according to claim 7, it is characterised in that Lactobacillus plantarum is effective in the feed preblending agent
Viable bacteria content is 3 × 108~8 × 109CFU/ml。
9. the application of the feed preblending agent according to claim 7 or 8, it is characterised in that by the feed preblending agent according to
The 2%~5% of feeding quality is added in feed, and is premixed before feeding.
10. the application of the feed preblending agent according to claim 7 or 8, it is characterised in that 1~2 hour in advance in sealing
Being premixed in container activates feed preblending agent anaerobism under the conditions of 20 DEG C~30 DEG C after the feed preblending agent and feed, premix.
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| CN105360609A (en) * | 2015-10-22 | 2016-03-02 | 无锡中科活力生物技术有限公司 | Fodder premix and preparation method thereof |
| CN110881579A (en) * | 2019-12-24 | 2020-03-17 | 江西省科学院生物资源研究所 | Pig intestinal function regulator and preparation method and application thereof |
| CN114128787B (en) * | 2021-12-02 | 2022-07-08 | 北京联合大学 | Nostoc commune functional feed and preparation method thereof |
| CN115462459B (en) * | 2022-09-13 | 2024-01-05 | 北京嘉博文生物科技有限公司 | Pig feed based on fermented product obtained after dining table residual food is treated |
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| CN1989830B (en) * | 2005-12-28 | 2011-12-21 | 上海创博生态工程有限公司 | Microbiological feed addictive special for micropterus salmonoides and method for making the same |
| CN101085982A (en) * | 2006-06-11 | 2007-12-12 | 上海创博生态工程有限公司 | Preparation method for producing microbe polysaccharides formulation using lactobacillus and microzyme |
| CN100581378C (en) * | 2006-08-01 | 2010-01-20 | 济南亿民动物药业有限公司 | Method for preparing fermented Chinese herbal medicine preparations for feed |
| CN101401921B (en) * | 2008-10-30 | 2011-11-23 | 河南省龙腾高科实业有限公司 | Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof |
| CN102232474B (en) * | 2011-04-15 | 2013-02-27 | 北京康华远景科技有限公司 | Disease-resistant growth promoter for aquatic animals and preparation method thereof |
| CN102669486B (en) * | 2012-06-11 | 2014-02-26 | 内蒙古永业农丰生物科技有限责任公司 | Special animal biogen for shrimps and preparation method thereof |
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2014
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