CN104450533A - Method for preparing lyophyllum decastes liquid strain by using rape straws as raw material - Google Patents
Method for preparing lyophyllum decastes liquid strain by using rape straws as raw material Download PDFInfo
- Publication number
- CN104450533A CN104450533A CN201410634051.XA CN201410634051A CN104450533A CN 104450533 A CN104450533 A CN 104450533A CN 201410634051 A CN201410634051 A CN 201410634051A CN 104450533 A CN104450533 A CN 104450533A
- Authority
- CN
- China
- Prior art keywords
- raw material
- bottle
- liquid strain
- culturing
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a method for preparing a lyophyllum decastes liquid strain by using rape straws as a raw material. The preparation method has low preparation cost, high inoculation efficiency and high strain germination speed, and is capable of reducing the pollution rate of a culture bottle.
Description
Technical field
The present invention relates to a kind of pilose antler mushroom liquid strain preparation method.
Background technology
Pilose antler mushroom is also known as lyophyllum decaste (Lyophyllum decastes (Fr.: Fr.) Sing.), and lotus leaf mushroom, is also called cold fragrant bacterium in the Yunnan of China, a brood of sheep, Fungus Pleurotus ostreatus etc., belong to Basidiomycotina, Hymenomycetes, cap order, Bai Mo section, from Agaricus, be called fried chicken mushroom in Europe, bacterial context is plump, fine and smooth, A sweety scent assails the nostrils, delicious flavour, research shows that crude protein in pilose antler massee fruiting bodies, amino acid whose content are higher, and lipid content is lower; But also contain useful trace element zinc, copper and the selenium of human body and a large amount of VITMAIN B1, B2, B6, B12 and nicotinic acid, there is very high nutritive value.Show according to modern scientific research, the beta glucan that pilose antler mushroom contains, long-term eating has antineoplastic effect, meanwhile, pilose antler mushroom have hypotensive, reduce the pharmacologic action such as cholesterol, anti-diabetic, antianaphylaxis, can strengthening by means of tonics, strengthen the body resistance to consolidate the constitution, strengthen immunity, delay senility.
The domestic research for the cultivation of pilose antler mushroom at present concentrates in the research of biological character mostly, and less to cultivation research, within 2007, Shanghai Finc Bio-tech Inc. reports industrialized cultivation pilose antler mushroom first.This article is pointed out to guarantee that the low stain rate that factory culture brings benefits is that cultivation is successfully crucial simultaneously.The link of Environmental capacity most critical is that bacterial classification can realize surely growing fast on culture material, and the vigor of bacterial classification is then surely grow most important factor fast.Inner Mongolia Autonomous Region in 2008 Yakeshi City Yi Tuli river forestry bureau feels that lyophyllum decaste (pilose antler mushroom) cultivating method patent has been applied in careless Xun forest farm, adopt solid spawn planting type, solid spawn preparation cost is long, and inoculation efficiency is low, bacterial classification germination physiology is slow, and the pollution rate of culturing bottle is high.
Summary of the invention
The invention provides a kind of preparation cost low, inoculation efficiency, bacterial classification germination physiology, reduce the pilose antler mushroom liquid strain preparation method of culturing bottle pollution rate.
The technical solution used in the present invention is: a kind of take Rape Straw as the pilose antler mushroom liquid strain preparation method of raw material, it is characterized in that:
(1) culture medium raw material is taken by mass fraction: Rape Straw 40-60 part, sorghum husk 20-50 part, rice bran 10-30 part, wheat bran 10-15 part, vinasse 10-20 part, calcium carbonate 1-3 part, calcium superphosphate 1-3 part, urea 1-3 part, sulphur ammonium 1-3 part, Nutrition Soil 10-15 part, water 155-255 part;
(2) step (1) raw material grinding mill is broken into diameter 0.1-0.3 centimetre of particle mixing, control pH value 6-7, load culturing bottle, every bottle of 500-700 part, gland encapsulates;
(3) vacreation pot sent into by culturing bottle, suction 50-80KPa after ozone sterilization 1-2 hour, sterilization 80-150 minute at 130 DEG C;
(4) cool at 15-20 DEG C, after cooling, send into every bottle graft original seed 35-50 bottle;
(5) 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3500PPM, cultivates 25-30 days.
Compared with prior art, advantage of the present invention is: substratum is easy to make, cost is low, and when making for liquid spawn, it is fast that bacterial classification sends out bacterium speed, pollution-free, without old mycoderma, is conducive to improving and sends out bacterium effect and output.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment one
A kind of take Rape Straw as the pilose antler mushroom liquid strain preparation method of raw material:
(1) culture medium raw material is taken by mass fraction: Rape Straw 40 parts, sorghum husk 40 parts, 20 parts, rice bran, 10 parts, wheat bran, 10 parts, vinasse, 1 part, calcium carbonate, calcium superphosphate 1 part, 1 part, urea, sulphur ammonium 1 part, Nutrition Soil 10 parts, 155 parts, water;
(2) step (1) raw material grinding mill is broken into diameter 0.3 centimetre of particle mixing, control pH value 6, load culturing bottle, 500 parts every bottle, gland encapsulates;
(3) vacreation pot sent into by culturing bottle, and ozone sterilization is suction 50KPa after 1 hour, sterilization 100 minutes at 130 DEG C;
(4) cool at 15 DEG C, after cooling, send into 35 bottles, every bottle graft original seed;
(5) 20 DEG C of culturing room, humidity 70%, between gas concentration lwevel 1600PPM, cultivates 30 days.
Embodiment two
A kind of take Rape Straw as the pilose antler mushroom liquid strain preparation method of raw material:
(1) culture medium raw material is taken by mass fraction: Rape Straw 60 parts, sorghum husk 30 parts, 30 parts, rice bran, 15 parts, wheat bran, 20 parts, vinasse, 3 parts, calcium carbonate, calcium superphosphate 3 parts, 3 parts, urea, sulphur ammonium 3 parts, Nutrition Soil 15 parts, 245 parts, water;
(2) step (1) raw material grinding mill is broken into diameter 0.1 centimetre of particle mixing, control pH value 6, load culturing bottle, 600 parts every bottle, gland encapsulates;
(3) vacreation pot sent into by culturing bottle, and ozone sterilization is suction 80KPa after 2 hours, sterilization 150 minutes at 130 DEG C;
(4) cool at 20 DEG C, after cooling, send into every bottle graft original seed 50;
(5) 25 DEG C of culturing room, humidity 80%, between gas concentration lwevel 3500PPM, cultivates 25 days.
Claims (1)
1. be a pilose antler mushroom liquid strain preparation method for raw material with Rape Straw, it is characterized in that:
(1) culture medium raw material is taken by mass fraction: Rape Straw 40-60 part, sorghum husk 20-50 part, rice bran 10-30 part, wheat bran 10-15 part, vinasse 10-20 part, calcium carbonate 1-3 part, calcium superphosphate 1-3 part, urea 1-3 part, sulphur ammonium 1-3 part, Nutrition Soil 10-15 part, water 155-255 part;
(2) step (1) raw material grinding mill is broken into diameter 0.1-0.3 centimetre of particle mixing, control pH value 6-7, load culturing bottle, every bottle of 500-700 part, gland encapsulates;
(3) vacreation pot sent into by culturing bottle, suction 50-80KPa after ozone sterilization 1-2 hour, sterilization 80-150 minute at 130 DEG C;
(4) cool at 15-20 DEG C, after cooling, send into every bottle graft original seed 35-50 bottle;
(5) 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3500PPM, cultivates 25-30 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410634051.XA CN104450533A (en) | 2014-11-12 | 2014-11-12 | Method for preparing lyophyllum decastes liquid strain by using rape straws as raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410634051.XA CN104450533A (en) | 2014-11-12 | 2014-11-12 | Method for preparing lyophyllum decastes liquid strain by using rape straws as raw material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104450533A true CN104450533A (en) | 2015-03-25 |
Family
ID=52897292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410634051.XA Pending CN104450533A (en) | 2014-11-12 | 2014-11-12 | Method for preparing lyophyllum decastes liquid strain by using rape straws as raw material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104450533A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105218197A (en) * | 2015-11-02 | 2016-01-06 | 江苏农牧科技职业学院 | A kind of pilose antler mushroom cultivation medium formula and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070111243A (en) * | 2006-05-17 | 2007-11-21 | 경북전문대학 산학협력단 | Culture medium and cultivation method for lyophyllum decastes |
| CN102726209A (en) * | 2012-05-13 | 2012-10-17 | 云南福保农业科技开发有限公司 | Method for artificially cultivating Oudemansiella radicata var.alba |
| CN103011945A (en) * | 2012-10-16 | 2013-04-03 | 上海丰科生物科技股份有限公司 | Lyophyllum decastes solid strain medium formula and strain production method |
| CN103202175A (en) * | 2013-02-07 | 2013-07-17 | 上海丰科生物科技股份有限公司 | Novel strain of lyophyllum decastes |
-
2014
- 2014-11-12 CN CN201410634051.XA patent/CN104450533A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070111243A (en) * | 2006-05-17 | 2007-11-21 | 경북전문대학 산학협력단 | Culture medium and cultivation method for lyophyllum decastes |
| CN102726209A (en) * | 2012-05-13 | 2012-10-17 | 云南福保农业科技开发有限公司 | Method for artificially cultivating Oudemansiella radicata var.alba |
| CN103011945A (en) * | 2012-10-16 | 2013-04-03 | 上海丰科生物科技股份有限公司 | Lyophyllum decastes solid strain medium formula and strain production method |
| CN103202175A (en) * | 2013-02-07 | 2013-07-17 | 上海丰科生物科技股份有限公司 | Novel strain of lyophyllum decastes |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105218197A (en) * | 2015-11-02 | 2016-01-06 | 江苏农牧科技职业学院 | A kind of pilose antler mushroom cultivation medium formula and preparation method thereof |
| CN105218197B (en) * | 2015-11-02 | 2018-07-13 | 江苏农牧科技职业学院 | A kind of pilose antler mushroom cultivation medium formula and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104350954A (en) | Lyophyllum decastes cultivation method | |
| CN108118020A (en) | Culture medium, preparation and its application of cellulose degradation microorganism | |
| CN104402623A (en) | Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method | |
| CN103570440A (en) | Edible mushroom culture medium adopting banana skin as raw material | |
| CN103011945A (en) | Lyophyllum decastes solid strain medium formula and strain production method | |
| CN106665044A (en) | Cultivation method of selenium-enriched tomato | |
| CN103570411A (en) | Edible fungus culture medium using dragon fruit peel as raw material | |
| CN104446789A (en) | Velvet antler mushroom cultivation formula | |
| CN102948325A (en) | Cordyceps militaris efficient quick cultivation technology | |
| CN104355913A (en) | Liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. | |
| CN104509688A (en) | Method of producing biological feed by fermenting banana stem and leaf through micro-organism | |
| CN104355915A (en) | Preparation method of liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. | |
| CN107266168A (en) | A kind of culture medium of edible fungus and cultivation method for edible mushroom | |
| CN104450539A (en) | Preparation method of velvet antler mushroom liquid strain | |
| CN104212720A (en) | Method for producing microalgae biomass by using monosodium glutamate ion-exchanged mother liquid | |
| CN104478571A (en) | Preparation method of liquid velvet antler mushroom strain | |
| CN104450533A (en) | Method for preparing lyophyllum decastes liquid strain by using rape straws as raw material | |
| CN107580974B (en) | Multi-stubble oyster mushroom fruiting device and mushroom fruiting method using same | |
| CN104478572A (en) | Formula of velvet antler mushroom culture medium | |
| CN104774049A (en) | Organic fertilizer for crops | |
| CN104839719A (en) | Method for producing biologically-fermented lepidium meyenii walp food | |
| CN101099435A (en) | Industrial cultivation method for Gloeostereum incarnatum S. Ito et Imai | |
| CN104365377A (en) | Velvet mushroom liquid strain preparation method with wood shavings as raw materials | |
| CN102972201A (en) | High-efficiency and fast cultivation technique of north worm grass | |
| CN104788149A (en) | Preparation technology of organic fertilizer for crop |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150325 |
|
| WD01 | Invention patent application deemed withdrawn after publication |