CN104355913A - Liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. - Google Patents
Liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. Download PDFInfo
- Publication number
- CN104355913A CN104355913A CN201410700134.4A CN201410700134A CN104355913A CN 104355913 A CN104355913 A CN 104355913A CN 201410700134 A CN201410700134 A CN 201410700134A CN 104355913 A CN104355913 A CN 104355913A
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- CN
- China
- Prior art keywords
- parts
- pilose antler
- magnesium sulfate
- bottle
- sulfate heptahydrate
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. The liquid medium comprises 10-30 parts of sucrose, 2-5 parts of soybean meal, 1-3 parts of beef extract, 0.3-1 part of magnesium sulfate heptahydrate, 0.3-1 part of potassium dihydrogen phosphate and 0.1-0.3 part of defoaming agent by mass. The medium has the advantages that the medium is convenient to prepare and is low in cost; when the medium is used for preparing liquid spawns, the spawns are fast in growth, are not polluted and do not have mycoderma, thus being conducive to improving the spawn growth effects and increasing the yield.
Description
Technical field
The present invention relates to a kind of pilose antler mushroom liquid nutrient medium and preparation method, liquid spawn preparation method.
Background technology
Pilose antler mushroom is also known as lyophyllum decaste (Lyophyllum decastes (Fr.: Fr.) Sing.), and lotus leaf mushroom, is also called cold fragrant bacterium in the Yunnan of China, a brood of sheep, Fungus Pleurotus ostreatus etc., belong to Basidiomycotina, Hymenomycetes, cap order, Bai Mo section, from Agaricus, be called fried chicken mushroom in Europe, bacterial context is plump, fine and smooth, A sweety scent assails the nostrils, delicious flavour, research shows that crude protein in pilose antler massee fruiting bodies, amino acid whose content are higher, and lipid content is lower; But also contain useful trace element zinc, copper and the selenium of human body and a large amount of VITMAIN B1, B2, B6, B12 and nicotinic acid, there is very high nutritive value.Show according to modern scientific research, the beta glucan that pilose antler mushroom contains, long-term eating has antineoplastic effect, meanwhile, pilose antler mushroom have hypotensive, reduce the pharmacologic action such as cholesterol, anti-diabetic, antianaphylaxis, can strengthening by means of tonics, strengthen the body resistance to consolidate the constitution, strengthen immunity, delay senility.
The domestic research for the cultivation of pilose antler mushroom at present concentrates in the research of biological character mostly, and less to cultivation research, within 2007, Shanghai Finc Bio-tech Inc. reports industrialized cultivation pilose antler mushroom first.This article is pointed out to guarantee that the low stain rate that factory culture brings benefits is that cultivation is successfully crucial simultaneously.The link of Environmental capacity most critical is that bacterial classification can realize surely growing fast on culture material, and the vigor of bacterial classification is then surely grow most important factor fast.Inner Mongolia Autonomous Region in 2008 Yakeshi City Yi Tuli river forestry bureau feels that lyophyllum decaste (pilose antler mushroom) cultivating method patent has been applied in careless Xun forest farm, adopt solid spawn planting type, solid spawn preparation cost is long, and inoculation efficiency is low, bacterial classification germination physiology is slow, and the pollution rate of culturing bottle is high.
Summary of the invention
The invention provides a kind of preparation cost low, inoculation efficiency, bacterial classification germination physiology, reduce the preparation method of the pilose antler mushroom liquid nutrient medium of culturing bottle pollution rate and substratum thereof, bacterial classification.
The technical solution used in the present invention is: a kind of pilose antler mushroom liquid nutrient medium, is characterized in that: comprise sucrose 10-30 part, dregs of beans 2-5 part, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3-1 part, potassium primary phosphate 0.3-1 part, defoamer 0.1-0.3 part by mass fraction.
Further, sucrose 20 parts, dregs of beans 3.3 parts, extractum carnis 2 parts, magnesium sulfate heptahydrate 0.5 part, potassium primary phosphate 0.5 part, defoamer 0.1 part is comprised by mass fraction.
A preparation method for pilose antler mushroom liquid nutrient medium, is characterized in that, comprise the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 10-30 part, dregs of beans 2-5 part, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3-1 part, potassium primary phosphate 0.3-1 part, defoamer 0.1-0.3 part;
(2), by step (1) raw material mix, control pH value 5.5-6.5, load culturing bottle, every bottle of 400-500 part, gland encapsulates;
(3), culturing bottle sends into vacreation pot, suction 50-80KPa after ozone sterilization 1-2 hour, insulation 30 minutes at 115 DEG C, insulation 60 minutes at 121 DEG C.
A preparation method for pilose antler mushroom liquid strain, is characterized in that, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 10-30 part, dregs of beans 2-5 part, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3-1 part, potassium primary phosphate 0.3-1 part, defoamer 0.1-0.3 part;
(2), by step (1) raw material mix, control pH value 5.5-6.5, load culturing bottle, every bottle of 400-500 part, gland encapsulates;
(3), culturing bottle sends into vacreation pot, suction 50-80KPa after ozone sterilization 1-2 hour, insulation 30 minutes at 115 DEG C, insulation 60 minutes at 121 DEG C.
(4), at 15-20 DEG C cool, after cooling, send into every bottle graft original seed 40-50;
(5), 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3200PPM, cultivates 20-25 days.
Compared with prior art, advantage of the present invention is: substratum is easy to make, cost is low, and when making for liquid spawn, it is fast that bacterial classification sends out bacterium speed, pollution-free, without old mycoderma, is conducive to improving and sends out bacterium effect and output.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment one
A kind of pilose antler mushroom liquid nutrient medium, comprises sucrose 10 parts, dregs of beans 2 parts, extractum carnis 1 part, magnesium sulfate heptahydrate 0.3 part, potassium primary phosphate 0.3 part, defoamer 0.1 part by mass fraction.
The preparation method of pilose antler mushroom liquid nutrient medium, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 10 parts, dregs of beans 2 parts, extractum carnis 1 part, magnesium sulfate heptahydrate 0.3 part, potassium primary phosphate 0.3 part, defoamer 0.1 part;
(2), by step (1) raw material mix, control pH value 5.5, load culturing bottle, 400 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 50KPa after 1 hour, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
The preparation method of pilose antler mushroom liquid strain, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 10 parts, dregs of beans 2 parts, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3 part, potassium primary phosphate 0.3 part, defoamer 0.1 part;
(2), by step (1) raw material mix, control pH value 5.5, load culturing bottle, 400 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 50KPa after 1 hour, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
(4), at 15 DEG C cool, after cooling, send into every bottle graft original seed 40;
(5), 20 DEG C of culturing room, humidity 70%, between gas concentration lwevel 1600PPM, cultivates 20 days.
Embodiment two
A kind of pilose antler mushroom liquid nutrient medium, comprises sucrose 30 parts, dregs of beans 5 parts, extractum carnis 3 parts, magnesium sulfate heptahydrate 1 part, potassium primary phosphate 1 part, defoamer 0.3 part by mass fraction.
The preparation method of pilose antler mushroom liquid nutrient medium, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 30 parts, dregs of beans 5 parts, extractum carnis 3 parts, magnesium sulfate heptahydrate 1 part, potassium primary phosphate 1 part, defoamer 0.3 part.；
(2), by step (1) raw material mix, control pH value 6.5, load culturing bottle, 500 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 80KPa after 2 hours, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
The preparation method of pilose antler mushroom liquid strain, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 30 parts, dregs of beans 5 parts, extractum carnis 3 parts, magnesium sulfate heptahydrate 1 part, potassium primary phosphate 1 part, defoamer 0.3 part.；
(2), by step (1) raw material mix, control pH value 6.5, load culturing bottle, 500 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 80KPa after 2 hours, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
(4), at 20 DEG C cool, after cooling, send into every bottle graft original seed 50;
(5), 25 DEG C of culturing room, humidity 80%, between gas concentration lwevel 3200PPM, cultivates 25 days.
Embodiment three
A kind of pilose antler mushroom liquid nutrient medium, comprises sucrose 20 parts, dregs of beans 3.3 parts, extractum carnis 2 parts, magnesium sulfate heptahydrate 0.5 part, potassium primary phosphate 0.5 part, defoamer 0.1 part by mass fraction.
A preparation method for pilose antler mushroom liquid nutrient medium, is characterized in that, comprise the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 20 parts, dregs of beans 3.3 parts, extractum carnis 2 parts, magnesium sulfate heptahydrate 0.5 part, potassium primary phosphate 0.5 part, defoamer 0.1 part;
(2), by step (1) raw material mix, control pH value 6, load culturing bottle, 500 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 70KPa after 2 hours, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
A preparation method for pilose antler mushroom liquid strain, is characterized in that, comprises the following steps:
(1), culture medium raw material is taken by mass fraction: sucrose 20 parts, dregs of beans 3.3 parts, extractum carnis 2 parts, magnesium sulfate heptahydrate 0.5 part, potassium primary phosphate 0.5 part, defoamer 0.1 part;
(2), by step (1) raw material mix, control pH value 6, load culturing bottle, 500 parts every bottle, gland encapsulates;
(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 70KPa after 2 hours, is incubated 30 minutes at 115 DEG C, is incubated 60 minutes at 121 DEG C.
(4), at 18 DEG C cool, after cooling, send into every bottle graft original seed 45;
(5), 22 DEG C of culturing room, humidity 75%, between gas concentration lwevel 2200PPM, cultivates 22 days.
Claims (2)
1. a pilose antler mushroom liquid nutrient medium, is characterized in that: comprise sucrose 10-30 part, dregs of beans 2-5 part, extractum carnis 1-3 part, magnesium sulfate heptahydrate 0.3-1 part, potassium primary phosphate 0.3-1 part, defoamer 0.1-0.3 part by mass fraction.
2. a kind of pilose antler mushroom liquid nutrient medium according to claim 1, is characterized in that: comprise sucrose 20 parts, dregs of beans 3.3 parts, extractum carnis 2 parts, magnesium sulfate heptahydrate 0.5 part, potassium primary phosphate 0.5 part, defoamer 0.1 part by mass fraction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410700134.4A CN104355913A (en) | 2014-11-28 | 2014-11-28 | Liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410700134.4A CN104355913A (en) | 2014-11-28 | 2014-11-28 | Liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. |
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| CN104355913A true CN104355913A (en) | 2015-02-18 |
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| CN201410700134.4A Pending CN104355913A (en) | 2014-11-28 | 2014-11-28 | Liquid medium for lyophyllum decastes (Fr. : Fr.) Sing. |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104402623A (en) * | 2014-11-28 | 2015-03-11 | 江苏菇本堂生物科技股份有限公司 | Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method |
| CN106047718A (en) * | 2016-06-27 | 2016-10-26 | 合肥福泉现代农业科技有限公司 | Cockroach-powder-based Ramaria botrytoides liquid strain culture medium and Ramaria botrytoides liquid strain preparation method |
| CN112125732A (en) * | 2020-09-19 | 2020-12-25 | 江苏丰收菇业有限公司 | Velvet antler mushroom liquid stock culture solution and velvet antler mushroom stock preparation process |
| CN112136602A (en) * | 2020-09-19 | 2020-12-29 | 灌南云农食用菌研究所(有限合伙) | Liquid cultivation culture solution and liquid cultivation production process for velvet antler mushrooms |
Citations (6)
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|---|---|---|---|---|
| JP2001327220A (en) * | 1991-09-24 | 2001-11-27 | Takara Shuzo Co Ltd | Method for artificiallly cultivating lyophyllum decastes |
| CN1923997A (en) * | 2005-11-17 | 2007-03-07 | 魏生龙 | Lyophyllum decastes edible fungus spawn |
| CN102907252A (en) * | 2011-08-05 | 2013-02-06 | 魏生龙 | Pilot-scale test fermentation process of mycelia of lyophyllum decastes |
| CN103202175A (en) * | 2013-02-07 | 2013-07-17 | 上海丰科生物科技股份有限公司 | Novel strain of lyophyllum decastes |
| CN104087548A (en) * | 2013-04-01 | 2014-10-08 | 梁倩倩 | Preparation and regeneration method of lyophyllum decastes protoplast |
| CN104402623A (en) * | 2014-11-28 | 2015-03-11 | 江苏菇本堂生物科技股份有限公司 | Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method |
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Patent Citations (6)
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| CN1923997A (en) * | 2005-11-17 | 2007-03-07 | 魏生龙 | Lyophyllum decastes edible fungus spawn |
| CN102907252A (en) * | 2011-08-05 | 2013-02-06 | 魏生龙 | Pilot-scale test fermentation process of mycelia of lyophyllum decastes |
| CN103202175A (en) * | 2013-02-07 | 2013-07-17 | 上海丰科生物科技股份有限公司 | Novel strain of lyophyllum decastes |
| CN104087548A (en) * | 2013-04-01 | 2014-10-08 | 梁倩倩 | Preparation and regeneration method of lyophyllum decastes protoplast |
| CN104402623A (en) * | 2014-11-28 | 2015-03-11 | 江苏菇本堂生物科技股份有限公司 | Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104402623A (en) * | 2014-11-28 | 2015-03-11 | 江苏菇本堂生物科技股份有限公司 | Lyophyllum decastes liquid medium and preparation method, liquid spawn preparation method |
| CN106047718A (en) * | 2016-06-27 | 2016-10-26 | 合肥福泉现代农业科技有限公司 | Cockroach-powder-based Ramaria botrytoides liquid strain culture medium and Ramaria botrytoides liquid strain preparation method |
| CN112125732A (en) * | 2020-09-19 | 2020-12-25 | 江苏丰收菇业有限公司 | Velvet antler mushroom liquid stock culture solution and velvet antler mushroom stock preparation process |
| CN112136602A (en) * | 2020-09-19 | 2020-12-29 | 灌南云农食用菌研究所(有限合伙) | Liquid cultivation culture solution and liquid cultivation production process for velvet antler mushrooms |
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Application publication date: 20150218 |