CN104478571A - Preparation method of liquid velvet antler mushroom strain - Google Patents

Abstract

The invention relates to a preparation method of a liquid velvet antler mushroom strain. The preparation method is characterized by comprising the following steps: (1) weighing culture medium raw materials in parts by weight: 40-60 parts of sawdust, 10-20 parts of rice bran, 5-10 parts of bran, 10-20 parts of cottonseed shells, 1-3 parts of lime, 10-15 parts of nutrient soil and 80-110 parts of water; (2) mixing the raw materials in the step (1) to obtain a mixture, controlling the pH value of the mixture to be 6-7, feeding 400-500 parts of the mixture into each culture flask, and sealing by a gland; (3) conveying the fed culture flasks into a vacuum sterilizing pot, sterilizing with ozone for 1-2 hours, and vacuumizing at 50-80KPa, and sterilizing at 150 DEG C for 100-120 minutes; (4) cooling at 15-20 DEG C, adding 1-4 parts of humic acid, and inoculating 40-50 parts of a source stock to each bottle after cooling; and (5) cultivating for 20-25 days by controlling the temperature of a culture room to be 20-25 DEG C, the humidity to be 70%-80% and the concentration of carbon dioxide to be 1600-3200PPM.

Description

Pilose antler mushroom liquid strain preparation method

Technical field

The present invention relates to a kind of pilose antler mushroom liquid strain preparation method.

Background technology

Pilose antler mushroom is also known as lyophyllum decaste (Lyophyllum decastes (Fr.: Fr.) Sing.), and lotus leaf mushroom, is also called cold fragrant bacterium in the Yunnan of China, a brood of sheep, Fungus Pleurotus ostreatus etc., belong to Basidiomycotina, Hymenomycetes, cap order, Bai Mo section, from Agaricus, be called fried chicken mushroom in Europe, bacterial context is plump, fine and smooth, A sweety scent assails the nostrils, delicious flavour, research shows that crude protein in pilose antler massee fruiting bodies, amino acid whose content are higher, and lipid content is lower; But also contain useful trace element zinc, copper and the selenium of human body and a large amount of VITMAIN B1, B2, B6, B12 and nicotinic acid, there is very high nutritive value.Show according to modern scientific research, the beta glucan that pilose antler mushroom contains, long-term eating has antineoplastic effect, meanwhile, pilose antler mushroom have hypotensive, reduce the pharmacologic action such as cholesterol, anti-diabetic, antianaphylaxis, can strengthening by means of tonics, strengthen the body resistance to consolidate the constitution, strengthen immunity, delay senility.

The domestic research for the cultivation of pilose antler mushroom at present concentrates in the research of biological character mostly, and less to cultivation research, within 2007, Shanghai Finc Bio-tech Inc. reports industrialized cultivation pilose antler mushroom first.This article is pointed out to guarantee that the low stain rate that factory culture brings benefits is that cultivation is successfully crucial simultaneously.The link of Environmental capacity most critical is that bacterial classification can realize surely growing fast on culture material, and the vigor of bacterial classification is then surely grow most important factor fast.Inner Mongolia Autonomous Region in 2008 Yakeshi City Yi Tuli river forestry bureau feels that lyophyllum decaste (pilose antler mushroom) cultivating method patent has been applied in careless Xun forest farm, adopt solid spawn planting type, solid spawn preparation cost is long, and inoculation efficiency is low, bacterial classification germination physiology is slow, and the pollution rate of culturing bottle is high.

Summary of the invention

The invention provides a kind of preparation cost low, inoculation efficiency, bacterial classification germination physiology, reduce the pilose antler mushroom liquid strain preparation method of culturing bottle pollution rate.

The technical solution used in the present invention is: a kind of pilose antler mushroom liquid strain preparation method, is characterized in that:

(1), by mass fraction culture medium raw material is taken: wood chip 40-60 part, rice bran 10-20 part, wheat bran 5-10 part, continuous seed shell 10-20 part, lime 1-3 part, Nutrition Soil 10-15 part, water 80-110 part;

(2), by step (1) raw material mix, control pH value 6-7, load culturing bottle, every bottle of 400-500 part, gland encapsulates;

(3), culturing bottle sends into vacreation pot, suction 50-80KPa after ozone sterilization 1-2 hour, sterilization 100-120 minute at 150 DEG C;

(4), at 15-20 DEG C cool, add humic acid 1-4 part, after cooling, send into every bottle graft original seed 40-50;

(5), 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3200PPM, cultivates 20-25 days.

Compared with prior art, advantage of the present invention is: substratum is easy to make, cost is low, and when making for liquid spawn, it is fast that bacterial classification sends out bacterium speed, pollution-free, without old mycoderma, is conducive to improving and sends out bacterium effect and output.Before connecing original seed, add humic acid can effectively avoid destroy humic acid early stage, improves follow-up rate of buddingging, guarantees bacterium mushroom output.

Embodiment

Below in conjunction with embodiment, the invention will be further described.

Embodiment one

A kind of pilose antler mushroom liquid strain preparation method:

(1), by mass fraction culture medium raw material is taken: wood chip 40 parts, 10 parts, rice bran, 5 parts, wheat bran, 10 parts, continuous seed shell, 1 part, lime, Nutrition Soil 10 parts, 80 parts, water;

(2), by step (1) raw material mix, control pH value 6, load culturing bottle, 400 parts every bottle, gland encapsulates;

(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 50KPa after 1 hour, sterilization 100 minutes at 150 DEG C;

(4), at 15 DEG C cool, add humic acid 1 part, after cooling, send into every bottle graft original seed 40;

(5), 20 DEG C of culturing room, humidity 70%, between gas concentration lwevel 1600PPM, cultivates 20 days.

Embodiment two

A kind of pilose antler mushroom liquid strain preparation method:

(1), by mass fraction culture medium raw material is taken: wood chip 60 parts, 20 parts, rice bran, 10 parts, wheat bran, 20 parts, continuous seed shell, 3 parts, lime, Nutrition Soil 15 parts, 110 parts, water;

(2), by step (1) raw material mix, control pH value 7, load culturing bottle, 500 parts every bottle, gland encapsulates;

(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 80KPa after 2 hours, sterilization 120 minutes at 150 DEG C;

(4), at 20 DEG C cool, add humic acid 2 parts, after cooling, send into every bottle graft original seed 50;

(5), 25 DEG C of culturing room, humidity 80%, between gas concentration lwevel 3200PPM, cultivates 25 days.

Embodiment three

A kind of pilose antler mushroom liquid strain preparation method:

(1), by mass fraction culture medium raw material is taken: wood chip 50 parts, 15 parts, rice bran, 8 parts, wheat bran, 15 parts, continuous seed shell, 2 parts, lime, Nutrition Soil 13 parts, 90 parts, water;

(2), by step (1) raw material mix, control pH value 6, load culturing bottle, 450 parts every bottle, gland encapsulates;

(3), culturing bottle sends into vacreation pot, and ozone sterilization is suction 60KPa after 1.5 hours, sterilization 110 minutes at 150 DEG C;

(4), at 18 DEG C cool, add humic acid 4 parts, after cooling, send into every bottle graft original seed 46;

(5), 22 DEG C of culturing room, humidity 78%, between gas concentration lwevel 2200PPM, cultivates 22 days.

Claims (1)

1. a pilose antler mushroom liquid strain preparation method, is characterized in that:

(1), by mass fraction culture medium raw material is taken: wood chip 40-60 part, rice bran 10-20 part, wheat bran 5-10 part, continuous seed shell 10-20 part, lime 1-3 part, Nutrition Soil 10-15 part, water 80-110 part;

(2), by step (1) raw material mix, control pH value 6-7, load culturing bottle, every bottle of 400-500 part, gland encapsulates;

(3), culturing bottle sends into vacreation pot, suction 50-80KPa after ozone sterilization 1-2 hour, sterilization 100-120 minute at 150 DEG C;

(4), at 15-20 DEG C cool, add humic acid 1-4 part, after cooling, send into every bottle graft original seed 40-50;

(5), 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3200PPM, cultivates 20-25 days.