CN104443469B - A kind of full-automatic sterile packaging process of culture medium - Google Patents
A kind of full-automatic sterile packaging process of culture medium Download PDFInfo
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- CN104443469B CN104443469B CN201410607558.6A CN201410607558A CN104443469B CN 104443469 B CN104443469 B CN 104443469B CN 201410607558 A CN201410607558 A CN 201410607558A CN 104443469 B CN104443469 B CN 104443469B
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- plate
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C7/00—Concurrent cleaning, filling, and closing of bottles; Processes or devices for at least two of these operations
- B67C7/0073—Sterilising, aseptic filling and closing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67B—APPLYING CLOSURE MEMBERS TO BOTTLES JARS, OR SIMILAR CONTAINERS; OPENING CLOSED CONTAINERS
- B67B3/00—Closing bottles, jars or similar containers by applying caps
- B67B3/02—Closing bottles, jars or similar containers by applying caps by applying flanged caps, e.g. crown caps, and securing by deformation of flanges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C3/00—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
- B67C3/007—Applications of control, warning or safety devices in filling machinery
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C3/00—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
- B67C3/02—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus
- B67C3/22—Details
- B67C3/24—Devices for supporting or handling bottles
Abstract
The invention discloses the full-automatic sterile packaging process of a kind of culture medium, this packaging process is by the empty ware feeding culture medium solution fill closing lid rewinding step of culture medium solution conveying；Realize culture medium solution fill integration and automation process, standardized work can be realized, efficiently solve pollution problem in pouring process, ensureing culture medium solution aseptic, whole fill flow process is totally enclosed type, thus improves production efficiency, reduce waste product, defect rate, increase economic benefit.
Description
Technical field
The present invention relates to the full-automatic sterile packaging process of a kind of sterile filling culture medium solution.
Background technology
Pre-fill agar culture medium flat board is mainly used in the microorganism monitoring of clean environment.Clean environment microorganism monitoring,
Refer to utilize microorganism under applicable temperature, humidity, nutritional condition, understand the feature of Fast-propagation, utilize specific culture medium
Flcating germ in clean environment, settling bacteria, body surface bacterium etc. are monitored cultivating, to determine the clean of clean environment by product
Clean the most satisfactory grade method.It is mainly used in the microorganism of the clean environment of pharmacy, food, cosmetics, medical industry
Monitoring Indexes, this just requires that culture medium plate has good susceptiveness and the most important index of aseptic the two.
Agar culture medium is as the important tool of clean environment microorganism monitoring, and itself must possess microorganism is wide
Spectrality, susceptiveness.Therefore, in himself production process, also have to strictly control its microbial limit for production environment and refer to
Mark, it is to avoid or reduce the pollution to the culture medium flat plate product in production process, ensure its aseptic.Although pre-fill agar is trained
Support base flat board can via gamma ray final sterilization, but higher irradiation dose can destroy some effective nutrition of culture medium
Composition, causes the sensitivity decrease of product, therefore, takes process control means as far as possible, reduce the initial contamination of culture medium product
Degree, and then the irradiation sterilization of low dosage, become ensure the susceptiveness of this product and two important indicators of aseptic simultaneously must
Select.
At present, the factor causing microorganism to be polluted is a lot, the ring when design of such as clean area and device layout, production
Border situation, all equipment relevant to production and the pollution situation of material, human users and sanitary condition etc., especially operator
Member, is maximum polluter, reduces the primary work that microorganism is polluted, it is simply that realize automated production as far as possible, reduce personnel's number
Amount, simultaneously by sensitive pouring process, is placed in the most airtight gnotobasis, reduces the wind that microorganism is polluted to greatest extent
Danger.
The most how to design the high filling apparatus of a kind of Totally-enclosed type sterile fill, production efficiency and method becomes current
Need the urgent problem solved.
Summary of the invention
The technical problem to be solved in the present invention is to overcome above-mentioned deficiency, it is provided that one uses aseptic totally enclosed type, automatically fills
The packaging process of dress culture medium solution.
In order to solve above-mentioned technical problem, it is carried out by the following technical programs:
A kind of packaging process of culture medium,
This packaging process comprises the following steps:
Step one: culture medium solution carries；
It is provided with pneumatic diaphragm bottom valve in culture medium sterile preparation tank base, uses pressurized with compressed air system to tank body
Internal pressurization, opens fill pipeline, the culture medium solution that will prepare, and exports tank body by pipeline, described protects tank body
It is to be realized by air compressor machine and air accumulator that pressure processes, and its compressed gas is oil-free, anhydrous, without granule, aseptic compressed air；
Step 2: empty ware feeding；
Sky ware is positioned over feeding device, and its feeding device lower end is provided with and automatically pushes away ware plate, automatically pushes away ware plate by whereabouts
Empty ware promotes forward, and described feeding device rear end is provided with suction cover device, and suction cover device is made up of bracing frame and at least eight sucker,
Automatically push away after sky ware is pushed to automatically open ware device by ware plate, by upper and lower sucker, sky ware upper cover is picked up, and utilize servo electricity
Machine moves forward, and is delivered to by ware lid on the main conveyor belt before sky ware body, and subsequently, empty ware body is pushed to by automatically pushing away ware plate
Fill platform main conveyor belt is fed forward, and empty ware body is 6-10cm with the spacing of upper cover, enters fill program；
Step 3: culture medium solution fill；
Feeding device side is additionally provided with filling apparatus, and described filling apparatus is made up of support, filling tube and filling exit, fill
Device uses automatic ration to follow filling mechanism, and these filling mechanism both sides are provided with step motor control dragging track, dragging track
Running simultaneously with fill platform, after empty ware body is fed forward to filling position, filling pump is opened and is carried out following fill, to be filled
To set amount, filling pump is closed and quickly returns initial position with dragging track, re-starts the fill of following of next ware, fills
Dress wild Oryza species plate is transmitted rearward by pipeline；
Step 4: closing lid；
The described fill platform other end is provided with pneumatic location push plate device and closing lid device, and pneumatic location push plate device is by extremely
Few semilune location push pedal, cylinder and stop composition, closing lid device is made up of support and sucker, and semilune location push pedal can profit
With cylinder promote move up and down, during off working state, push pedal is positioned at upper position, in order to plate from push pedal bottom with main conveying
Band moves forward.When upper cover enters push pedal lower end, semilune location, it is positioned at the photocell detection of side to signal, and feeds back to first quarter moon
Shape location push pedal moves down, and automatically promotes upper cover to hold upper cover to sucker lower end, sucker, waits the flat of fill culture medium
Ware body is with push pedal lower end, main conveyor belt entrance semilune location, and semilune location push pedal moves down, and automatically promotes plate originally
Body is to the sucker lower end of closing lid device, and now closing lid device does pressing action, by the upper cover alignment plate body closing lid of absorption also
Locking；
Step 5: rewinding and coding；
Described closing lid device one end is additionally provided with Pneumatic ledal-pushing device, and Pneumatic ledal-pushing device is made up of push pedal and stop, closes
After lid, plate is delivered to push pedal lower end, and push pedal moves down, and automatically promotes the rewinding conveyer belt that plate is the most vertical with main conveyor belt,
Rewinding conveyer belt is provided with the banking stop device of projection, it is ensured that every corresponding plate of banking stop also pushes forward to plate pressure
Locking device, press fit device will fail to be sufficiently complete the plate pressing again of closing lid lock out action.Complete the plate of pressing through rewinding
Conveyer belt is fed forward, and enters laser-induced thermal etching district, and laser head is positioned at below rewinding conveyer belt, according to pre-entering data, to warp
It is etched work bottom each the plate crossed.Continue on to automatic stacking device through overetched plate, automatically stack
Device can stack at least ten plate, whole fill EP (end of program) simultaneously.
Preferably, in step one, described feeding device side is provided with infrared induction equipment, for judging the often empty ware of group
Horizontal level line.
Preferably, in step one, feeding device is at least eight group feeding circular hole structures, described feeding groove diameter root
Set according to empty ware specification.
Preferably, in step 3, fill platform periphery is provided with RABs insulation blocking system, and fill platform upper end is provided with
System layer stream, the culture medium after fill, during transmitting, under the protection of insulation blocking system, blows downwards through system layer stream
Clean way flow wind carries out cooling down, solidifying.
Preferably, described automatic stacking device lower end is provided with screens conveying bar, and screens conveying bar is provided with projection, and plate enters
Entering rear flank on screens conveying bar, screens conveying bar is started upper shifting, is headed into automatic stacking device by plate by projection.
Using as above after technical scheme, it has the beneficial effect that
Take above-mentioned filling technological method, it is achieved culture medium solution fill integration and automation process, standard can be realized
Equalization, efficiently solves pollution problem in pouring process, it is ensured that culture medium solution aseptic, and whole fill flow process is totally-enclosed
Formula, thus improve production efficiency, reduce waste product, defect rate, Improving The Quality of Products.
The quality standard producing culture medium finished product according to this packaging process is as shown in table 1 below:
Table 1:
| Sequence number | Project | Technical specification | The method of inspection |
| 1 | Outward appearance | Surface wettability is smooth, is uniformly distributed, inviolateness and foreign body. | Range estimation |
| 2 | Color | Culture medium should be translucent, different according to culture medium kind, in Existing light amber or other colors | Range estimation |
| 3 | Thickness | ≥3mm | Vernier caliper measurement |
| 4 | Soft durometer | Scratching process is answered flawless or meets gel strength requirement | With inoculating loop in media surface Line or use gel strength instrument Measure |
| 5 | pH | The recipe requirements of each culture medium should be met | Liquid/solid pH meter measures |
| 6 | Aseptic | Under the conditions of 23～28 DEG C and 30～35 DEG C, each cultivation 7 days should nothing Bacteria growing | Put in biochemistry/constant incubator and train Perusal after supporting |
| 7 | The suitability | Inoculation escherichia coli [CMCC (B) 44102/ATCC8739], Staphylococcus aureus [CMCC (B) 26003/ATCC6538], Bacillus subtilis [CMCC (B) 63501/ATCC6633], copper Green pseudomonas [CMCC (B) 10104/ATCC9027] is in 30 ～35 DEG C of cultivations numeration in 48 hours, average colony number is not less than right According to 70% of the average colony number in culture medium, and colonial morphology In the same size. | Put after constant incubator is cultivated Perusal |
| 7 | The suitability | Inoculation Candida albicans [CMCC (F) 98001/ATCC10231], Aspergillus niger [CMCC (F) 98003/ ATCC16404] in 23～ Cultivating numeration in 72 hours for 28 DEG C, average colony number is not less than comparison 70% of average colony number in culture medium, and colonial morphology is big Little unanimously. | Put after constant incubator is cultivated Perusal |
Accompanying drawing explanation
Fig. 1 is the full-automatic sterile filling process flow chart of culture medium of the present invention.
Detailed description of the invention
The present invention is described in detail with detailed description of the invention below in conjunction with the accompanying drawings, it is impossible to is not understood as the present invention's
Limit；
As it is shown in figure 1, the full-automatic sterile packaging process of a kind of culture medium, this packaging process comprises the following steps:
Step one: culture medium solution carries；
It is provided with pneumatic tank bottom valve in culture medium sterile preparation tank base, uses pressurized with compressed air system to tank interior
Supercharging, opens fill pipeline, by pipeline, the culture medium solution after preparation is discharged tank body, described tank body is carried out pressurize process
Being to be realized by air compressor machine and air accumulator, its compressed gas is oil-free, anhydrous, without granule, aseptic compressed air；
Step 2: empty ware feeding；
Sky ware is positioned over feeding device, and its feeding device lower end is provided with and automatically pushes away ware plate, automatically pushes away ware plate by whereabouts
Empty ware promotes forward.Described feeding device rear end is provided with suction cover device, and suction cover device is made up of bracing frame and at least eight sucker,
Automatically push away after sky ware is pushed to automatically open ware device by ware plate, by upper and lower sucker, sky ware upper cover is picked up, and utilize servo electricity
Machine moves forward, and is delivered to by ware lid on the main conveyor belt before sky ware body, and subsequently, empty ware body is pushed to by automatically pushing away ware plate
Fill platform main conveyor belt is fed forward, and empty ware body is 6-10cm with the spacing of upper cover, enters fill program；
Step 3: culture medium solution fill；
Feeding device side is additionally provided with filling apparatus, and described filling apparatus is made up of support, filling tube and filling exit, fill
Device uses automatic ration to follow filling mechanism, and these filling mechanism both sides are provided with step motor control dragging track, dragging track
Running simultaneously with fill platform, after empty ware body is fed forward to filling position, filling pump is opened and is carried out following fill, to be filled
To set amount, filling pump is closed and quickly returns initial position with dragging track, re-starts the fill of following of next ware, fills
Dress wild Oryza species plate is transmitted rearward by pipeline；
Step 4: closing lid；
The described fill platform other end is provided with pneumatic location push plate device and closing lid device, and pneumatic location push plate device is by extremely
Few semilune location push pedal, cylinder and stop composition, closing lid device is made up of support and sucker, and semilune location push pedal can profit
With cylinder promote move up and down, during off working state, push pedal is positioned at upper position, in order to plate from push pedal bottom with main conveying
Band moves forward.When upper cover enters push pedal lower end, semilune location, it is positioned at the photocell detection of side to signal, and feeds back to first quarter moon
Shape location push pedal moves down, and automatically promotes upper cover to hold upper cover to sucker lower end, sucker, waits the flat of fill culture medium
Ware body is with push pedal lower end, main conveyor belt entrance semilune location, and semilune location push pedal moves down, and automatically promotes plate originally
Body is to the sucker lower end of closing lid device, and now closing lid device does pressing action, by the upper cover alignment plate body closing lid of absorption also
Locking；
Step 5: rewinding and coding；
Described closing lid device one end is additionally provided with Pneumatic ledal-pushing device, and Pneumatic ledal-pushing device is made up of push pedal and stop, closes
After lid, plate is delivered to push pedal lower end, and push pedal moves down, and automatically promotes the rewinding conveyer belt that plate is the most vertical with main conveyor belt,
Rewinding conveyer belt is provided with the banking stop device of projection, it is ensured that every corresponding plate of banking stop also pushes forward to plate pressure
Locking device, press fit device will fail to be sufficiently complete the plate pressing again of closing lid lock out action.Complete the plate of pressing through rewinding
Conveyer belt is fed forward, and enters laser-induced thermal etching district, and laser head is positioned at below rewinding conveyer belt, according to pre-entering data, to warp
It is etched work bottom each the plate crossed.Continue on to automatic stacking device through overetched plate, automatically stack
Device can stack at least ten plate, whole fill EP (end of program) simultaneously.
Claims (5)
1. the full-automatic sterile packaging process of a culture medium, it is characterised in that
This packaging process comprises the following steps:
Step one: culture medium solution carries；
It is provided with pneumatic tank bottom valve in culture medium sterile preparation tank base, uses pressurized with compressed air system that tank interior is increased
Pressure, opens fill pipeline, and by pipeline, the culture medium solution after preparation is discharged tank body, and tank body carries out pressurize processing is to pass through
Air compressor machine and air accumulator realize, and its compressed gas is oil-free, anhydrous, without granule, aseptic compressed air；
Step 2: empty ware feeding；
Sky ware is positioned over feeding device, and its feeding device lower end is provided with and automatically pushes away ware plate, automatically pushes away the empty ware that ware plate will fall
Promoting forward, described feeding device rear end is provided with suction cover device, and suction cover device is made up of, automatically bracing frame and at least eight sucker
Push away after sky ware is pushed to automatically open ware device by ware plate, by upper and lower sucker, sky ware upper cover is picked up, and utilize servomotor to fill
Putting and move forward, delivered to by ware lid on the main conveyor belt before sky ware body, subsequently, empty ware body is pushed to fill by automatically pushing away ware plate
Platform main conveyor belt is fed forward, and empty ware body is 6-10cm with the spacing of upper cover, enters fill program；
Step 3: culture medium solution fill；
Feeding device side is additionally provided with filling apparatus, and described filling apparatus is made up of support, filling tube and filling exit, filling apparatus
Using automatic ration to follow filling mechanism, these filling mechanism both sides are provided with step motor control dragging track, dragging track and filling
Assembling platform runs simultaneously, and after empty ware body is fed forward to filling position, filling pump is opened and carried out following fill, to be filled to setting
After Ding Liang, filling pump is closed and quickly returns initial position with dragging track, re-starts the fill of following of next ware, after fill
Culture medium plate is transmitted rearward by pipeline；
Step 4: closing lid；
The described fill platform other end is provided with pneumatic location push plate device and closing lid device, and pneumatic location push plate device is by not a half
Moon shape location push pedal, cylinder and stop composition, closing lid device is made up of support and sucker, and semilune location push pedal may utilize gas
Cylinder promotes and moves up and down, and during off working state, push pedal is positioned at upper position, in order to plate is before push pedal bottom is with main conveyor belt
Move, when upper cover enters push pedal lower end, semilune location, be positioned at the photocell detection of side to signal, and feed back to semilune calmly
Position push pedal moves down, and automatically promotes upper cover to hold upper cover to sucker lower end, sucker, waits the plate of fill culture medium originally
Body enters push pedal lower end, semilune location with main conveyor belt, and semilune location push pedal moves down, and automatically promotes plate body extremely
The sucker lower end of closing lid device, now closing lid device does pressing action, the upper cover of absorption is directed at plate body closing lid and locks；
Step 5: rewinding and coding；
Described closing lid device one end is additionally provided with Pneumatic ledal-pushing device, and Pneumatic ledal-pushing device is made up of push pedal and stop, after closing lid
Plate is delivered to push pedal lower end, and push pedal moves down, and automatically promotes plate to the rewinding conveyer belt vertical with main conveyor belt, rewinding
Conveyer belt is provided with the banking stop device of projection, it is ensured that every corresponding plate of banking stop also pushes forward to plate pressing dress
Putting, press fit device will fail to be sufficiently complete the plate pressing again of closing lid lock out action, and the plate completing pressing carries through rewinding
Band is fed forward, and enters laser-induced thermal etching district, and laser head is positioned at below rewinding conveyer belt, according to pre-entering data, to process
It is etched work bottom each plate, continues on to automatic stacking device, automatic stacking device through overetched plate
At least ten plate, whole fill EP (end of program) can be stacked simultaneously.
The full-automatic sterile packaging process of culture medium the most according to claim 1, it is characterised in that: in step 2, institute
State feeding device and be provided with infrared induction equipment near lower end position, for judging the often empty ware horizontal level line of group, if any
One assembles doses is less than infrared induction equipment, and according to program setting, fill action can stop.
The full-automatic sterile packaging process of culture medium the most according to claim 1, it is characterised in that: in step 2, on
Material device has at least eight group feeding circular hole structures, is used for controlling plate entrance and automatically pushes away ware device, and described feeding circular hole is tied
Structure diameter sets according to empty ware specification size.
The full-automatic sterile packaging process of culture medium the most according to claim 1, it is characterised in that: in step 3, fill
Assembling platform periphery is provided with RABs insulation blocking system, and fill platform upper end is provided with system layer stream, and the culture medium after fill is transmitting
During, under the protection of insulation blocking system, blow downwards clean way flow wind through system layer stream and carry out cooling down, solidifying.
The full-automatic sterile packaging process of culture medium the most according to claim 1, it is characterised in that: described automatic heap closed assembly
Putting lower end and be provided with screens conveying bar, screens conveying bar is provided with projection, and plate enters rear flank on screens conveying bar, screens conveying bar
Move in startup, by projection, plate is headed into automatic stacking device.
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| CN104443469B true CN104443469B (en) | 2016-08-24 |
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| CN105907834A (en) * | 2016-03-09 | 2016-08-31 | 浙江天杭生物科技股份有限公司 | Pre-preparation culture plate and its preparation method and quality control method |
| JP6725031B2 (en) * | 2019-04-10 | 2020-07-15 | 大日本印刷株式会社 | Culture device and culture method |
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| CN202807120U (en) * | 2012-09-06 | 2013-03-20 | 杭州泰林生物技术设备有限公司 | Pouring dish device for culture medium |
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| US6202713B1 (en) * | 1997-03-08 | 2001-03-20 | Forschungszentrum Julich Gmbh | Method and device for series cultivation of organisms |
| KR20020011697A (en) * | 2000-08-03 | 2002-02-09 | 백우인 | Automatic device for discharging badges in plastic bags |
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Denomination of invention: A fully automatic aseptic filling method of culture medium Effective date of registration: 20230103 Granted publication date: 20160824 Pledgee: Bank of China Limited by Share Ltd. Taizhou branch Pledgor: JIANGSU YIMA BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2023980030111 |