CN104443469A - Full-automatic sterile filling method for culture medium - Google Patents
Full-automatic sterile filling method for culture medium Download PDFInfo
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- CN104443469A CN104443469A CN201410607558.6A CN201410607558A CN104443469A CN 104443469 A CN104443469 A CN 104443469A CN 201410607558 A CN201410607558 A CN 201410607558A CN 104443469 A CN104443469 A CN 104443469A
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- filling
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C7/00—Concurrent cleaning, filling, and closing of bottles; Processes or devices for at least two of these operations
- B67C7/0073—Sterilising, aseptic filling and closing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67B—APPLYING CLOSURE MEMBERS TO BOTTLES JARS, OR SIMILAR CONTAINERS; OPENING CLOSED CONTAINERS
- B67B3/00—Closing bottles, jars or similar containers by applying caps
- B67B3/02—Closing bottles, jars or similar containers by applying caps by applying flanged caps, e.g. crown caps, and securing by deformation of flanges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C3/00—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
- B67C3/007—Applications of control, warning or safety devices in filling machinery
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C3/00—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus; Filling casks or barrels with liquids or semiliquids
- B67C3/02—Bottling liquids or semiliquids; Filling jars or cans with liquids or semiliquids using bottling or like apparatus
- B67C3/22—Details
- B67C3/24—Devices for supporting or handling bottles
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a full-automatic sterile filling method for a culture medium. The filling method includes the steps of culture medium solution conveyance, empty container feeding, culture medium solution filling, cover closing and collection. The integrated and automatic culture medium solution filling process is achieved, standard operation can be achieved, the pollution problem existing in the filling process is effectively solved, sterilization of a culture medium solution is guaranteed, the whole filling procedure is fully closed, production efficiency is improved, the rejection rate and the defective rate are decreased, and economic benefits are increased.
Description
Technical field
The present invention relates to a kind of full-automatic sterile packaging process of sterile filling culture medium solution.
Background technology
Pre-filling agar medium flat board is mainly used in the microorganism monitoring of clean environment.Clean environment microorganism monitoring, refer to and utilize germ under the temperature be applicable to, humidity, nutritional condition, the feature of meeting Fast-propagation, utilize specific culture product, carry out monitoring to the flcating germ in clean environment, settling bacteria, body surface bacterium etc. to cultivate, to determine the whether satisfactory method of the lustration class of clean environment.Be mainly used in pharmacy, food, cosmetics, medical industry clean environment microbiological indicator monitoring, this just requires that culture plate has good sensitieness and these two most important indexs of aseptic.
Agar medium, as the important tool of clean environment microorganism monitoring, itself must possess broad spectrum activity, the sensitieness to germ.Therefore, in himself production process, also strictly must control its microbial limit index for production environment, avoid or reduce, to the pollution of the culture medium flat plate product in production process, to ensure its aseptic.Although pre-filling agar medium flat board can via gamma ray final sterilization, but higher irradiation dose can destroy some effective nutritional labelings of culture, cause the sensitivity decrease of product, therefore, take process control means as far as possible, reduce the initial contamination degree of culture product, and then the irradiation sterilization of low dosage, become the necessary choice of sensitieness and aseptic two important indicators simultaneously ensureing this product.
At present, cause the factor of microbial contamination a lot, the environmental aspect when design of such as clean area and device layout thereof, production, all to produce the pollution situation of relevant equipment and material, human users and sanitary condition etc., especially operating personal, maximum source of pollution, reduce the primary work of microbial contamination, be exactly realize automated production as far as possible, reduce personnel amount, simultaneously by the pouring process of sensitivity, be placed in highly airtight gnotobasis, reduce the risk of microbial contamination to greatest extent.
So how to design a kind of Totally-enclosed type sterile is filling, production efficiency is high filling apparatus and method to become and need the urgent problem solved at present.
Summary of the invention
The technical problem to be solved in the present invention overcomes above-mentioned deficiency, provides a kind of packaging process adopting aseptic totally enclosed, auto-filling culture medium solution.
In order to solve the problems of the technologies described above, implemented by the following technical programs:
A packaging process for culture,
This packaging process comprises the following steps:
Step one: culture medium solution is carried;
Pneumatic diaphragm bottom valve is provided with in culture sterile preparation tank base, adopt pressurized with compressed air system to tank interior supercharging, open filling pipeline, by the culture medium solution prepared, tank body is exported by pipeline, it is described that to carry out pressurize process to tank body be realized by Air compressor and storage tank, its pressure gas be nothing oily, anhydrous, without particle, aseptic compressed air;
Step 2: empty ware material loading;
Empty ware is positioned over feeding device, its feeding device lower end is provided with and automatically pushes away ware plate, automatically push away ware plate the empty ware fallen is promoted forward, described feeding device rear end is provided with suction cover device, suction cover device is made up of bracing frame and at least eight suckers, automatically push away after empty ware is pushed to and automatically opens ware device by ware plate, by upper and lower sucker, empty ware upper cover is picked up, and utilize servo motor device to move forward, ware lid is delivered on the main conveyor belt before sky ware body, subsequently, empty ware body is automatically pushed away ware plate and pushes to filling platform main conveyor belt and be fed forward, the spacing of empty ware body and upper cover is 6-10cm, enter filling program,
Step 3: culture medium solution is filling;
Feeding device side is also provided with filling apparatus, described filling apparatus is made up of support, filling tube and filling exit, filling apparatus adopts automatic ration to follow filling mechanism, these filling mechanism both sides are provided with step motor control dragging track, dragging track and filling platform synchronized operation, after empty ware body is fed forward to filling position, filling pump open carry out following filling, to be filled to set amount, filling pump close and with dragging track fast return initial position, re-start following of next ware filling, filling wild Oryza species plate is transmitted rearward by travel line;
Step 4: closing lid;
The described filling platform other end is provided with pneumatic location push plate device and closing lid device, pneumatic location push plate device is made up of at least half moon location push pedal, cylinder and limiting device, closing lid device is made up of support and sucker, half moon location push pedal can utilize cylinder to promote to move up and down, during off working state, push pedal is positioned at upper position, so that plate moves forward from push pedal bottom with main conveyor belt.When upper cover enters push pedal lower end, half moon location, be positioned at the photocell detection of side to signal, and feed back to half moon location push pedal move down, automatic promotion upper cover is to sucker lower end, sucker holds upper cover, wait for that the plate body of filling culture enters push pedal lower end, half moon location with main conveyor belt, half moon location push pedal moves down, automatic promotion plate body is to the sucker lower end of closing lid device, now closing lid device does pressing action, the upper cover of absorption is aimed at plate body closing lid and locks;
Step 5: rewinding and coding;
Described closing lid device one end is also provided with Pneumatic ledal-pushing device, Pneumatic ledal-pushing device is made up of push pedal and limiting device, after closing lid, plate is delivered to push pedal lower end, push pedal moves down, automatic promotion plate is to the rewinding belt conveyor vertical with main conveyor belt, rewinding belt conveyor is provided with protruding banking stop device, ensures the corresponding plate of every banking stop and pushes forward to plate press fit device, and press fit device will fail fully to complete the plate pressing again of closing lid lock out action.The plate completing pressing is fed forward through rewinding load-transfer device, enters laser-induced thermal etching district, and laser head is positioned at below rewinding load-transfer device, according to pre-entering data, carries out etching work to bottom each plate of process.Continue on to automatic stacking device through overetched plate, automatic stacking device can simultaneously stacking at least ten plates, whole filling end of program.
Preferably, in step one, described feeding device side is provided with infrared induction equipment, often organizes empty ware level attitude line for judging.
Preferably, in step one, feeding device is at least eight group material loading circular hole structures, and described material loading groove diameter sets according to empty ware specification.
Preferably, in step 3, outside filling platform, be arranged with RABs insulation blocking system; filling platform upper end is provided with system layer stream; culture after filling, in transport process, under the protection of insulation blocking system, blows clean way flow wind downwards through system layer stream and carries out cooling, solidifying.
Preferably, described automatic stacking device lower end is provided with screens conveying bar, and screens conveying bar is provided with projection, and plate enters rear flank on screens conveying bar, moves, headed into plate in automatic stacking device by projection on screens conveying bar starts.
Adopt as above after technical scheme, its beneficial effect is:
Take above-mentioned filling technological method, realize the filling integration of culture medium solution and automation process, standardized work can be realized, efficiently solve pollution problem in pouring process, ensure culture medium solution aseptic, whole filling flow process is totally enclosed, thus enhances productivity, reduce waste product, fraction defect, Improving The Quality of Products.
The quality loop producing culture finished product according to this packaging process is as shown in table 1 below:
Table 1:
| sequence number | project | technical index | the method of inspection |
| 1 | outward appearance | surface wettability is smooth, is uniformly distributed, inviolateness and foreign matter. | range estimation |
| 2 | color | culture should be translucent, different according to culture kind, presents light amber or other colors | range estimation |
| 3 | thickness | >=3mm | vernier caliper measurement |
| 4 | soft durometer | scratching process is answered flawless or is met gel strength requirement | measure in media surface line or use gel strength instrument with oese |
| 5 | pH | the recipe requirements of each culture should be met | liquid/solid pH meter measures |
| 6 | aseptic | under 23 ~ 28 DEG C and 30 ~ 35 DEG C of conditions, each cultivation answers asepsis growth in 7 days | put visual inspection after cultivating in biochemistry/constant incubator |
| 7 | applicability | inoculation EHEC [CMCC (B) 44102/ ATCC8739], staphylococcus aureus [CMCC (B) 26003/ ATCC6538], bacillus subtilis [CMCC (B) 63501/ ATCC6633], pseudomonas aeruginosa [CMCC (B) 10104/ ATCC9027] cultivate numeration in 48 hours in 30 ~ 35 DEG C, average colony number is not less than 70% of the average colony number in control medium, and colonial morphology is in the same size. | put visual inspection after cultivating in constant incubator |
| 7 | applicability | inoculation Candida albicans [CMCC (F) 98001/ ATCC10231], aspergillus niger [CMCC (F) 98003/ ATCC16404] cultivate numeration in 72 hours in 23 ~ 28 DEG C, average colony number is not less than 70% of the average colony number in control medium, and colonial morphology is in the same size. | put visual inspection after cultivating in constant incubator |
Accompanying drawing explanation
Fig. 1 is the full-automatic sterile filling process diagram of circuit of culture of the present invention.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail, can not be interpreted as it is limitation of the present invention;
As shown in Figure 1, a kind of full-automatic sterile packaging process of culture, this packaging process comprises the following steps:
Step one: culture medium solution is carried;
Pneumatic tank bottom valve is provided with in culture sterile preparation tank base, adopt pressurized with compressed air system to tank interior supercharging, open filling pipeline, culture medium solution after preparation is discharged tank body by pipeline, it is described that to carry out pressurize process to tank body be realized by Air compressor and storage tank, its pressure gas be without oily, anhydrous, without particle, aseptic pressurized air;
Step 2: empty ware material loading;
Empty ware is positioned over feeding device, and its feeding device lower end is provided with and automatically pushes away ware plate, automatically pushes away ware plate and is promoted forward by the empty ware fallen.Described feeding device rear end is provided with suction cover device, suction cover device is made up of bracing frame and at least eight suckers, automatically push away after empty ware is pushed to and automatically opens ware device by ware plate, by upper and lower sucker, empty ware upper cover is picked up, and utilize servo motor device to move forward, ware lid is delivered on the main conveyor belt before sky ware body, subsequently, empty ware body is automatically pushed away ware plate and pushes to filling platform main conveyor belt and be fed forward, and the spacing of empty ware body and upper cover is 6-10cm, enters filling program;
Step 3: culture medium solution is filling;
Feeding device side is also provided with filling apparatus, described filling apparatus is made up of support, filling tube and filling exit, filling apparatus adopts automatic ration to follow filling mechanism, these filling mechanism both sides are provided with step motor control dragging track, dragging track and filling platform synchronized operation, after empty ware body is fed forward to filling position, filling pump open carry out following filling, to be filled to set amount, filling pump close and with dragging track fast return initial position, re-start following of next ware filling, filling wild Oryza species plate is transmitted rearward by travel line;
Step 4: closing lid;
The described filling platform other end is provided with pneumatic location push plate device and closing lid device, pneumatic location push plate device is made up of at least half moon location push pedal, cylinder and limiting device, closing lid device is made up of support and sucker, half moon location push pedal can utilize cylinder to promote to move up and down, during off working state, push pedal is positioned at upper position, so that plate moves forward from push pedal bottom with main conveyor belt.When upper cover enters push pedal lower end, half moon location, be positioned at the photocell detection of side to signal, and feed back to half moon location push pedal move down, automatic promotion upper cover is to sucker lower end, sucker holds upper cover, wait for that the plate body of filling culture enters push pedal lower end, half moon location with main conveyor belt, half moon location push pedal moves down, automatic promotion plate body is to the sucker lower end of closing lid device, now closing lid device does pressing action, the upper cover of absorption is aimed at plate body closing lid and locks;
Step 5: rewinding and coding;
Described closing lid device one end is also provided with Pneumatic ledal-pushing device, Pneumatic ledal-pushing device is made up of push pedal and limiting device, after closing lid, plate is delivered to push pedal lower end, push pedal moves down, automatic promotion plate is to the rewinding belt conveyor vertical with main conveyor belt, rewinding belt conveyor is provided with protruding banking stop device, ensures the corresponding plate of every banking stop and pushes forward to plate press fit device, and press fit device will fail fully to complete the plate pressing again of closing lid lock out action.The plate completing pressing is fed forward through rewinding load-transfer device, enters laser-induced thermal etching district, and laser head is positioned at below rewinding load-transfer device, according to pre-entering data, carries out etching work to bottom each plate of process.Continue on to automatic stacking device through overetched plate, automatic stacking device can simultaneously stacking at least ten plates, whole filling end of program.
Claims (5)
1. a full-automatic sterile packaging process for culture, is characterized in that,
This packaging process comprises the following steps:
Step one: culture medium solution is carried;
Pneumatic tank bottom valve is provided with in culture sterile preparation tank base, adopt pressurized with compressed air system to tank interior supercharging, open filling pipeline, culture medium solution after preparation is discharged tank body by pipeline, it is described that to carry out pressurize process to tank body be realized by Air compressor and storage tank, its pressure gas be without oily, anhydrous, without particle, aseptic pressurized air;
Step 2: empty ware material loading;
Empty ware is positioned over feeding device, its feeding device lower end is provided with and automatically pushes away ware plate, automatically push away ware plate the empty ware fallen is promoted forward, described feeding device rear end is provided with suction cover device, suction cover device is made up of bracing frame and at least eight suckers, automatically push away after empty ware is pushed to and automatically opens ware device by ware plate, by upper and lower sucker, empty ware upper cover is picked up, and utilize servo motor device to move forward, ware lid is delivered on the main conveyor belt before sky ware body, subsequently, empty ware body is automatically pushed away ware plate and pushes to filling platform main conveyor belt and be fed forward, the spacing of empty ware body and upper cover is 6-10cm, enter filling program,
Step 3: culture medium solution is filling;
Feeding device side is also provided with filling apparatus, described filling apparatus is made up of support, filling tube and filling exit, filling apparatus adopts automatic ration to follow filling mechanism, these filling mechanism both sides are provided with step motor control dragging track, dragging track and filling platform synchronized operation, after empty ware body is fed forward to filling position, filling pump open carry out following filling, to be filled to set amount, filling pump close and with dragging track fast return initial position, re-start following of next ware filling, filling wild Oryza species plate is transmitted rearward by travel line;
Step 4: closing lid;
The described filling platform other end is provided with pneumatic location push plate device and closing lid device, pneumatic location push plate device is by least half moon location push pedal, cylinder and limiting device composition, closing lid device is made up of support and sucker, half moon location push pedal can utilize cylinder to promote to move up and down, during off working state, push pedal is positioned at upper position, so that plate moves forward from push pedal bottom with main conveyor belt, when upper cover enters push pedal lower end, half moon location, be positioned at the photocell detection of side to signal, and feed back to half moon location push pedal move down, automatic promotion upper cover is to sucker lower end, sucker holds upper cover, wait for that the plate body of filling culture enters push pedal lower end, half moon location with main conveyor belt, half moon location push pedal moves down, automatic promotion plate body is to the sucker lower end of closing lid device, now closing lid device does pressing action, the upper cover of absorption is aimed at plate body closing lid and locks,
Step 5: rewinding and coding;
Described closing lid device one end is also provided with Pneumatic ledal-pushing device, Pneumatic ledal-pushing device is made up of push pedal and limiting device, after closing lid, plate is delivered to push pedal lower end, push pedal moves down, automatic promotion plate is to the rewinding belt conveyor vertical with main conveyor belt, rewinding belt conveyor is provided with protruding banking stop device, ensure the corresponding plate of every banking stop and push forward to plate press fit device, press fit device will fail fully to complete the plate pressing again of closing lid lock out action, the plate completing pressing is fed forward through rewinding load-transfer device, enter laser-induced thermal etching district, laser head is positioned at below rewinding load-transfer device, according to pre-entering data, etching work is carried out to bottom each plate of process, automatic stacking device is continued on to through overetched plate, automatic stacking device can simultaneously stacking at least ten plates, whole filling end of program.
2. the full-automatic sterile packaging process of culture according to claim 1, it is characterized in that: in step one, described feeding device is provided with infrared induction equipment near lower end position, empty ware level attitude line is often organized for judging, if any assembling doses is lower than infrared induction equipment, according to program setting, filling action can stop.
3. the full-automatic sterile packaging process of culture according to claim 1, it is characterized in that: in step one, feeding device has at least eight group material loading circular hole structures, and enter automatically push away ware device for controlling plate, described material loading groove diameter sets according to empty ware specification size.
4. the full-automatic sterile packaging process of culture according to claim 1; it is characterized in that: in step 3; RABs insulation blocking system is arranged with outside filling platform; filling platform upper end is provided with system layer stream; culture after filling is in transport process; under the protection of insulation blocking system, blow clean way flow wind downwards through system layer stream and carry out cooling, solidifying.
5. the full-automatic sterile packaging process of culture according to claim 1, it is characterized in that: described automatic stacking device lower end is provided with screens conveying bar, screens conveying bar is provided with projection, plate enters rear flank on screens conveying bar, move on screens conveying bar starts, by projection, plate is headed into in automatic stacking device.
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| CN201410607558.6A CN104443469B (en) | 2014-11-03 | 2014-11-03 | A kind of full-automatic sterile packaging process of culture medium |
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| CN201410607558.6A CN104443469B (en) | 2014-11-03 | 2014-11-03 | A kind of full-automatic sterile packaging process of culture medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105907834A (en) * | 2016-03-09 | 2016-08-31 | 浙江天杭生物科技股份有限公司 | Pre-preparation culture plate and its preparation method and quality control method |
| JP2019107036A (en) * | 2019-04-10 | 2019-07-04 | 大日本印刷株式会社 | Culture device and method |
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| CN203904339U (en) * | 2014-05-04 | 2014-10-29 | 江苏益玛生物科技有限公司 | Culture dish capable of preventing pollution and culture medium shedding and provided with locking points |
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| US6202713B1 (en) * | 1997-03-08 | 2001-03-20 | Forschungszentrum Julich Gmbh | Method and device for series cultivation of organisms |
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| CN105907834A (en) * | 2016-03-09 | 2016-08-31 | 浙江天杭生物科技股份有限公司 | Pre-preparation culture plate and its preparation method and quality control method |
| JP2019107036A (en) * | 2019-04-10 | 2019-07-04 | 大日本印刷株式会社 | Culture device and method |
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| Publication number | Publication date |
|---|---|
| CN104443469B (en) | 2016-08-24 |
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Denomination of invention: A fully automatic aseptic filling method of culture medium Effective date of registration: 20230103 Granted publication date: 20160824 Pledgee: Bank of China Limited by Share Ltd. Taizhou branch Pledgor: JIANGSU YIMA BIOLOGICAL TECHNOLOGY Co.,Ltd. Registration number: Y2023980030111 |
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