CN104435202A - Preparation method for antioxidant extract - Google Patents
Preparation method for antioxidant extract Download PDFInfo
- Publication number
- CN104435202A CN104435202A CN201310417399.9A CN201310417399A CN104435202A CN 104435202 A CN104435202 A CN 104435202A CN 201310417399 A CN201310417399 A CN 201310417399A CN 104435202 A CN104435202 A CN 104435202A
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- CN
- China
- Prior art keywords
- sod
- extraction
- fructus chaenomelis
- papaya
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/732—Chaenomeles, e.g. flowering quince
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6949—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes
- A61K47/6951—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit inclusion complexes, e.g. clathrates, cavitates or fullerenes using cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a preparation method for an antioxidant extract. The method includes: at room temperature using water as an extraction solvent, chopping cleaned papaya, conducting smashing with a juicer according to a solid-liquid ratio of 1:2, carrying out assisted extraction respectively with microwave and ultrasonic equipment for 20s and 20min, then performing extraction for 1h, then filtering out coarse fiber parts with gauze, and conducting pressure reduced pumping filtering so as to obtain a papaya SOD crude extracted liquid; then removing some impure protein by heat modification to improve the purity of SOD, finally embedding the papaya extracted liquid with cyclodextrin, and carrying out freeze drying, thus obtaining extract dry powder. The method enables better utilization of Rosaceae papaya by means of SOD extraction, and turns waste into treasure. By adopting water, the extraction effect is better, and water serving as the extraction solvent has the advantages of cheapness, green and environmental protection. The method provided by the invention adopts the means of ultrasonic wave and microwave for the first time to improve the extraction effect.
Description
Technical field
The present invention relates to a kind of preparation method of antioxidant extract, particularly the preparation method of antioxidant extract.
Background technology
In China, Fructus Chaenomelis is used as medicine by as a kind of Chinese medicine always.With Fructus Chaenomelis soaked can wind dispelling, pain relieving.Containing abundant antioxidation class material in Fructus Chaenomelis, wherein superoxide dismutase, namely the SOD content known of people is also very high.SOD is a kind of protease, and in leaching process, the selection of environmental condition, Extraction solvent, extracting method all can affect its activity.The application of SOD mainly concentrates on inflammation patient, especially treats rheumatoid arthritis, chronic polyarthritis, myocardial infarction, cardiovascular diseases, tumor patient and radiation treatment inflammation patient.As a kind of biochemical enzyme reagents, be widely used in clinical and scientific research, can defying age, antitumor, regulate human body hormonal system.
Cultivated area is very large in locality, Mount Huang, and output is also very high, but in period of fruit maturation, there is a large amount of fruit well to be utilized, because outlet is narrow, preserve processed, soap etc. can only be used for, the economic benefit brought is not high, thus causes the waste of resource.
Summary of the invention
The invention provides a kind of preparation method of antioxidant extract, can the SOD in Fructus Chaenomelis be become to branch away high efficiency extraction.
For reaching above object, the present invention by the following technical solutions:
The present invention is at normal temperatures using water as extraction solvent, by the Fructus Chaenomelis chopping cleaned, smash according to the solid-liquid ratio juice extractor of 1:2, lixiviate 1h after assisted extraction 20s and 20min is carried out respectively with microwave and ultrasound instrument, then the pars fibrosa that gauze elimination is thicker is used, decompress filter, obtains Fructus Chaenomelis SOD crude extract.Then adopt thermal denaturation to remove some foreign proteins, improve the purity of SOD, finally embed Fructus Chaenomelis extracting solution with cyclodextrin, lyophilization obtains extract dried powder.
Beneficial effect of the present invention is:
The Flos Rosae Multiflorae Fructus Chaenomelis that the present invention is directed to Mount Huang optimizes extraction scheme, better can be utilized by the Flos Rosae Multiflorae Fructus Chaenomelis of locality with the form extracting SOD.SOD lixiviate prioritization scheme is in the past usually direct using saline solution as extracting solution, and does not consider the extracting effect of water.The present invention adopts the extracting effect of water to be better than saline solution, and water has cheaply as extraction solvent, the advantage of environmental protection.The present invention adopts ultrasound wave first, and the mode of microwave is carried out lixiviate and improved extracting effect.
Detailed description of the invention
1, the present invention is at normal temperatures using water as extraction solvent, by the Fructus Chaenomelis chopping cleaned, smash according to the solid-liquid ratio juice extractor of 1:2, lixiviate 1h after assisted extraction 20s and 20min is carried out respectively with microwave and ultrasound instrument, then the pars fibrosa that gauze elimination is thicker is used, decompress filter, obtains Fructus Chaenomelis SOD crude extract.
2, remove some foreign proteins by thermal denaturation, improve the purity of SOD
SOD is a kind of more heat-resisting protease, can keep active below 90 DEG C.Will degeneration but some other albumen is heated.The present invention has adopted the method heating lixiviating solution a period of time with 50 DEG C of water-baths, allows some foreign protein degeneration postprecipitations get off, thus reaches the object of preliminary purification SOD.
3, embed Fructus Chaenomelis extracting solution with cyclodextrin, lyophilization obtains extract dried powder
Although SOD is a kind of protease that can be heat-resisting, for a long time heat drying still can affect its activity.Freeze-drying is more advanced production method in the processing carrying out natural extract at present.Use freeze-drying, can heat, water is directly distilled, can not the activity of destructive enzyme.Using cycloheptaamylose as embedding medium, Fructus Chaenomelis SOD is embedded.If but convection drying SOD extracting solution, active unstable in SOD prolonged storage afterwards.The cyclodextrin (β-CD) that the present invention first adopts food to add rank before it is dried embeds the SOD enzyme isoreactivity material in Fructus Chaenomelis extracting solution.
Claims (4)
1. the preparation method of an antioxidant extract, it is characterized in that: the present invention carries out lixiviate using water as extraction solvent at normal temperatures, then thermal denaturation is adopted to remove some foreign proteins, improve the purity of SOD, finally embed Fructus Chaenomelis extracting solution with cyclodextrin, lyophilization obtains extract dried powder.
2. lixiviate according to claim 1, it is characterized in that: by the Fructus Chaenomelis chopping cleaned, smash according to the solid-liquid ratio juice extractor of 1:2, lixiviate 1h after assisted extraction 20s and 20min is carried out respectively with microwave and ultrasound instrument, then the pars fibrosa that gauze elimination is thicker is used, decompress filter, obtains Fructus Chaenomelis SOD crude extract.
3. method according to claim 1, is characterized in that: the present invention has adopted the method heating lixiviating solution a period of time with 50 DEG C of water-baths, allows some foreign protein degeneration postprecipitations get off, thus reaches the object of preliminary purification SOD.
4. method according to claim 1, it is characterized in that: the cyclodextrin (β-CD) that the present invention first adopts food to add rank before it is dried embeds the SOD enzyme isoreactivity material in Fructus Chaenomelis extracting solution, finally adopt freeze-drying to obtain extract dried powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310417399.9A CN104435202A (en) | 2013-09-13 | 2013-09-13 | Preparation method for antioxidant extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310417399.9A CN104435202A (en) | 2013-09-13 | 2013-09-13 | Preparation method for antioxidant extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104435202A true CN104435202A (en) | 2015-03-25 |
Family
ID=52882464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310417399.9A Pending CN104435202A (en) | 2013-09-13 | 2013-09-13 | Preparation method for antioxidant extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104435202A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105288652A (en) * | 2015-10-30 | 2016-02-03 | 珠海市雅莎生物科技有限公司 | Superoxide dismutase microcapsule powder and preparation method thereof |
-
2013
- 2013-09-13 CN CN201310417399.9A patent/CN104435202A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105288652A (en) * | 2015-10-30 | 2016-02-03 | 珠海市雅莎生物科技有限公司 | Superoxide dismutase microcapsule powder and preparation method thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150325 |
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| WD01 | Invention patent application deemed withdrawn after publication |