CN104432061B - biological embryonic cell nutrient - Google Patents
biological embryonic cell nutrient Download PDFInfo
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- CN104432061B CN104432061B CN201410795240.5A CN201410795240A CN104432061B CN 104432061 B CN104432061 B CN 104432061B CN 201410795240 A CN201410795240 A CN 201410795240A CN 104432061 B CN104432061 B CN 104432061B
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- linseed
- biological
- semen sesami
- sesami nigrum
- broccoli seeds
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 48
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to field of health care food, be specifically related to a kind of biological embryonic cell nutrient, this nutrient, obtains through biological cell culture technique for raw material with linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son; Wherein, with parts by weight, linseed 30-70 part, broccoli seeds 10-60 part, Semen sesami nigrum 10-50 part, oat seed 10-50 part, strange refined sub-10-50 part.Not only content is high for biological embryonic cell nutrient of the present invention, and very comprehensive, can provide human body comprehensive nutritional labeling, and has jointly given play to develop immunitypty, antifatigue, the function that delays senility, give protection against cancer.The present invention adopts biological cell broken wall superfine communication technique, and final product is easily absorbed by the body more.
Description
Technical field
The invention belongs to field of health care food, be specifically related to a kind of biological embryonic cell nutrient.
Background technology
The mankind need constantly pickuping food from external environment in vital movement process, and therefrom obtain the nutriment needed for vital movement, these materials are called nutrient, comprise carbohydrate, fat, protein, minerals and vitamins totally five large classes.Vitamin is the material that a class can participate in the multiple metabolic process of body, but it can not provide energy. also not as body constituent.Concerning most of vitamin, our body requirement amount is few, but can not lack.Most vitamin, body can not synthesize or synthetic quantity deficiency, can not meet the needs of body, constantly by obtaining in food.Mineral matter is the important component forming tissue, and having and regulate membrane passage and maintain excitatoty effect that is neural, musculature, is the composition of hormone, vitamin, protein and multiple enzyme.Medicine can not substitute nutrient, and it is not all right for depending merely on medicine and not carrying out the supplementary of corresponding nutrient.No matter the body in disease recovery, or the disease caused by nutritional deficiency, even in some chronic diseases, nutrient all serves very important effect.Chinese residents nutrition and health survey display in 2002, current Chinese population is micro-insufficiency of intake or unreasonable generally, not only result in various nutrients deficiency disease and occurs also may having close contacting with the generation of multiple chronic disease development.
China is as a developing country, and nutritional deficiency, under the prerequisite advocating balanced nutritious, rational diet, can improve undernutrition conditions, the generation of Chronic disease prevention thus the effect reaching antifatigue, delay senility by reasonable employment nutrient prime replenisher.Various nutrients just progressively pay close attention to by people and pay attention to.
Summary of the invention
For the problems referred to above, the invention provides and a kind ofly can supplement body trace element, develop immunitypty, antifatigue, delay senility, the biological embryonic cell nutrient of preventing cancer function, adopt biological embryonic cell culture technique, nutritional labeling in various raw material is significantly improved, adopt biological cell wall breaking technology that nutritional labeling is discharged completely, its product facilitates again the use of people, has supplementary body trace element, develop immunitypty, antifatigue, the function that delays senility, give protection against cancer.
Biological embryonic cell nutrient of the present invention, with linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son for raw material, obtains through biological cell culture technique; Wherein, with parts by weight, linseed 30-70 part, broccoli seeds 10-60 part, Semen sesami nigrum 10-50 part, oat seed 10-50 part, strange refined sub-10-50 part.
Linseed, not only nutrient composition content is high, and very comprehensive, mainly comprises nutritional labelings such as being rich in lignanoid's (content is up to 26%), Ω-3 polyunsaturated fatty acid, Organic Selenium, vitamin C, vitamin E, B family vitamin, folic acid.Abundant fiber is contained in lignanoid, can reduce the cancer incidence relevant with hormone, as breast cancer, and carcinoma of endometrium and prostate cancer.Fiber in food is divided into two classes, and one is non-soluble fiber, can reduce the intestinal transport time, very useful to constipation, and ight soil can be made to excrete more rapidly.Another kind is water-soluble fibre, can help to regulate blood sugar, reduces cholesterol concentration.Containing 2/3rds non-soluble fibers and 1/3rd water-soluble fibres in lignanoid, so it has both effects simultaneously.
Lanzhou University's laboratory research shows: lignanoid can breed and impel cancer cell-apoptosis by inhibition cancer cell, and to preventing, cancer of the stomach, knot (directly) intestinal cancer effect are the most obvious.In stomach cancer cell experiment body, add the lignanoid of variable concentrations, often organize cell and cultivate 24,48 and 72h respectively.Result shows, and in the experimental group adding lignanoid, its proliferation of human gastric cancer cell is obviously suppressed.Wherein in the experimental group of 72h process, inhibition of cancer cell rate is 19%; Cancer cell-apoptosis rate significantly increases, and contrast does not add the experimental group of lignanoid, and cancer cell-apoptosis rate adds 11%.
Ω-3 polyunsaturated fatty acid can: 1, reduce triglycerides and cholesterol, prevention of cardiac; 2, suppress platelet aggregation, prevent thrombosis and apoplexy; 3, improve brain cell active, prevent senile dementia; 4, blood sugar is reduced, prevention diabetes; 5, prevention of inflammation and cancer.
Vitamin C can resist the injury of radical pair cell, prevents cytometaplasia and cancer cell diffusion.
Vitamin E can protect body cell from the murder by poisoning of free radical, avoids biomembrane lipid peroxidation, causes membrane structure to be damaged.
B family vitamin can give protection against cancer.According to the data that American Cancer Society provides, B family vitamin can effectively inhibition cancer cell growth.
Folic acid then can priming cancer cell death, stops that sb.'s illness took a turn for the worse.
Authority's research finds, growing plants under extreme weather conditions---wild linseed in Western Canadian Arctic circle is higher than the linseed nutrient composition content in other area, therefore, and the present invention's preferred Canadian Field Park Company natural flax seed.
Broccoli seeds nutritional labeling is also very high, and mainly comprise protein, carbohydrate, fat, mineral composition, vitamin C and carrotene etc., wherein mineral composition more comprehensively, and calcium, phosphorus, iron, potassium, zinc, manganese equal size are all very abundant.
Semen sesami nigrum is the black seeds of Pedaliaceae sesame, containing a large amount of fat and protein, also has the nutritional labelings such as carbohydrate, VitAVitE, lecithin, calcium, iron, chromium.Nature and flavor: sweet are flat.Function cures mainly: filling liver kidney, benefiting essence-blood, and ease constipation is dry.For dizzy, Hiccough and deaf, poliosis, post disease alopecia, the dry constipation of intestines.The multiple essential amino acid that Semen sesami nigrum contains, under the effect of vitamin E and vitamin B1 participates in, can accelerate the metabolic function of human body; The iron that Semen sesami nigrum contains and vitamin E are the important component of prevention anaemia, activating brain cell, elimination blood vessel cholesterol; The fat that Semen sesami nigrum contains is mostly unrighted acid, has the effect of promoting longevity.
Traditional Chinese medicine theory is thought, Semen sesami nigrum has filling liver kidney, moisturizing viscera, beneficial strength, longue meat, fills out the effect of brains, can be used for treating dizzy, poliosis, alopecia, soreness and weakness of waist and knees, limbs fatigue caused by liver kidney asthenia of essence and blood, walk with difficulty, the illness such as deficient five internal organs, skin are dryly sent out withered, the dry constipation of intestines, effect in blacking hair and nourishing face, celebrated especially.
Oat can beneficial liver and stomach, for eat less caused by liver-stomach disharmony, receive poor, stool is not smooth, effectively can reduce the cholesterol in human body, often eat, certain prevention effect can be played to the chief threat-cardiovascular and cerebrovascular diseases of the elderly.Often edible oat also has effect of extraordinary hypoglycemic, fat-reducing to diabetic.Sowens has the effect of logical stool, and a lot of the elderly defecates dry, easily causes cerebrovas-cularaccident, and oat can separate this sorrow of constipation.It can also improve blood circulation, alleviates the pressure that life and work brings; Effect that the mineral matters such as the calcium contained, phosphorus, iron, zinc have prevention of osteoporosis, promote wound healing, prevent anaemia is good merchantable brand of replenishing the calcium.Containing extremely abundant linoleic acid in oat, also have auxiliary curative effect to fatty liver, diabetes, edema, constipation etc., strengthen muscle power to the elderly, it is also of great advantage for promoting longevity.
Strange refined son is the seed of chia, nutritious, is rich in 52 kinds of nutrients, and protein, calcium, iron, omega-3 and more than ten plant natural anti-reflecting oxide.Vitamin containing 45mg in every 100 Ke Qiya, wherein the ratio of crucial B family vitamin is more reasonable, i.e. the vitamin B-2 (riboflavin) of 2%, the niacin of 13%, the thiamine of 29%.Strange refined son has found the main component of all vitamin B complexes at least containing trace.In the strange refined son of every 100 grams, have the calcium of 631 milligrams, 100 grams of milk are only containing the calcium of 120 milligrams, and strange refined son is 5 times of its content, and phosphorus content is higher than milk 11 times, and potassium content is higher than milk 4.6 times.And strange refined sub-protein content is also 5-7 times of milk.The iron content of strange refined son is the highest in all seeds.Iron-holder is 6 times, 1.8 times of French beans, 2.4 times of beef liver of spinach.The strange refined son of 100g is containing the lipid of the 34g that has an appointment, and wherein Omega-3 is 19.3g, Omega-6 is 7.1g, is the food of the unique omega-3 content of nature more than omega-6, for protection cardiovascular and cerebrovascular, improves the compliance of blood vessel endothelium, has very great help.
Inventor studies discovery, and when with parts by weight, linseed 30-60 part, broccoli seeds 20-50 part, Semen sesami nigrum 20-40 part, oat seed 20-40 part, during strange refined sub-20-40 part, the biological embryonic cell nutrient effect of acquisition is better.
The concrete preparation process of described biological cell culture technique is:
(1) linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son carried out respectively screen, clean, removal of impurities;
(2) take linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son in proportion, put into cell culture fluid respectively, rotating and culturing 3-7 days at 20-40 DEG C of temperature;
(3) linseed after step (2) being cultivated, broccoli seeds, Semen sesami nigrum, oat seed, the vacuum drying at 20-50 DEG C respectively of strange refined son, make its water content reach 3%-10%;
(4) step (3) dried linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are used biological cell wall breaking technology ultramicro grinding respectively, make its sporoderm-broken rate reach more than 99%;
(5) linseed crushed in step (4), broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are put into mixer fully to mix, be filled to complex pocket and namely obtain biological embryonic cell nutrient.
Described cell culture fluid composition and ratio is as follows: lactose 20-40g/L, caseinhydrolysate 1-4g/L, dusty yeast 2-6g/L, potassium dihydrogen phosphate 0.5-4g/L, ferrous lactate 1-5g/L, sodium selenite 0.01-1.0g/L, vitamin B6 0.01-1.0g/L/, vitamin C 5-20g/L, surplus is water.
By linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son, put into above-mentioned cell culture fluid respectively to cultivate, can the nutritional labeling such as potassium, iron, selenium, dimension B6, Vc in enrichment nutrient solution, its nutritional labeling is multiplied, after adopting cell culture fluid to cultivate, after measured, in above-mentioned raw materials, B family vitamin B6 increases by 4.83 times, selenium increases by 8 times, vitamin C increases by 9.25 times, vitamin E increases by 9.66 times, and antioxidant activity improves 37 times.In order to make linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son at utmost absorb composition in nutrient solution, and just can grow plumule, and above-mentioned raw materials needs rotating and culturing 3-7 days at 20-40 DEG C of temperature.In order to make all kinds of raw materials in nutrient solution fully contact with nutrient solution, can better absorb composition in nutrient solution, the rotary rpm in step (2) is 50-200rpm.
In order to nutrient solution can better be made raw material submergence, make it absorb nutrition completely, cell culture fluid weight is 2-5:1 with the ratio of linseed, broccoli seeds, Semen sesami nigrum, oat seed, each weight of strange refined son.
In processing method of the present invention, according to the characteristic that raw material is seed, seed all has hard shell, especially Canadian Field Park Company natural flax seed has hard shell, if adopt Ordinary pulverization method to be difficult to active ingredient to discharge, therefore the present invention adopts biological cell broken wall superfine communication technique, active ingredient is discharged completely, make the effect that effect of product reaches maximum, after pulverizing with common grinder respectively by dried linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son, cross 100 mesh sieves; Then each raw meal airslide disintegrating mill crushed is carried out ultramicro grinding.In order to make seed Middle nutrition composition can completely nutritional labeling be discharged by ultramicro grinding, can better absorb nutritional labeling when human body is taken, rear grain fineness pulverized by raw material of the present invention is 3-5 μm, and sporoderm-broken rate reaches 99%.
The linseed crushed in step (4), broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are put into mixer fully to mix, be filled to complex pocket and namely obtain biological embryonic cell nutrient.
The nutritional labeling of the biological embryonic cell nutrient that the present invention obtains, not only content is high, and it is very comprehensive, mainly comprise nutritional labelings such as being rich in lignanoid, Ω-3 polyunsaturated fatty acid, Organic Selenium, vitamin C, vitamin E, B family vitamin, folic acid, these can provide human body comprehensive nutritional labeling, and have jointly given play to develop immunitypty, antifatigue, the function that delays senility, give protection against cancer.The present invention is cultivated raw material by biological embryonic cell culture technique, and have natural sex after making raw material absorb trace element, human body more easily absorbs.And the present invention adopts biological cell broken wall superfine communication technique, further increase the trap of human body to this product.
Detailed description of the invention
Embodiment 1
A kind of biological embryonic cell nutrient, is characterized in that: with linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son for raw material, obtains through biological cell culture technique; Wherein, with parts by weight, linseed 60 parts, broccoli seeds 50 parts, Semen sesami nigrum 40 parts, oat seed 40 parts, strange refined sub 40 parts.
The concrete preparation process of described biological cell culture technique is:
(1) linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son carried out respectively screen, clean, removal of impurities;
(2) take linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son in proportion, put into cell culture fluid respectively, rotating and culturing 7 days at 20 DEG C of temperature, rotary rpm is 50rpm; Cell culture fluid weight is respectively 5:1 with the ratio of linseed, broccoli seeds, Semen sesami nigrum, oat seed, each weight of strange refined son.
(3) linseed after step (2) being cultivated, broccoli seeds, Semen sesami nigrum, oat seed, the vacuum drying at 20-50 DEG C respectively of strange refined son, make its water content reach 3%-10%;
(4) step (3) dried linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are used biological cell wall breaking technology ultramicro grinding respectively, make its sporoderm-broken rate reach more than 99%, grain fineness reaches 3 μm;
(5) linseed crushed in step (4), broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are put into mixer fully to mix, be filled to complex pocket and namely obtain biological embryonic cell nutrient.
Described cell culture fluid composition and ratio is as follows: lactose 35g/L, caseinhydrolysate 3g/L, dusty yeast 4g/L, potassium dihydrogen phosphate 3g/L, ferrous lactate 5g/L, sodium selenite 0.08g/L, vitamin B6 0.06g/L/, vitamin C 12g/L, and surplus is water.
Described linseed is Canadian Field Park Company natural flax seed.
Embodiment 2
A kind of biological embryonic cell nutrient, is characterized in that: with linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son for raw material, obtains through biological cell culture technique; Wherein, with parts by weight, linseed 40 parts, broccoli seeds 20 parts, Semen sesami nigrum 20 parts, oat seed 30 parts, strange refined sub 30 parts.
The concrete preparation process of described biological cell culture technique is:
(1) linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son carried out respectively screen, clean, removal of impurities;
(2) take linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son in proportion, put into cell culture fluid respectively, rotating and culturing 3 days at 40 DEG C of temperature, rotary rpm is 200rpm; Cell culture fluid weight is respectively 3:1 with the ratio of linseed, broccoli seeds, Semen sesami nigrum, oat seed, each weight of strange refined son.
(3) linseed after step (2) being cultivated, broccoli seeds, Semen sesami nigrum, oat seed, the vacuum drying at 20-50 DEG C respectively of strange refined son, make its water content reach 3%-10%;
(4) step (3) dried linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are used biological cell wall breaking technology ultramicro grinding respectively, make its sporoderm-broken rate reach more than 99%, grain fineness reaches 5 μm;
(5) linseed crushed in step (4), broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are put into mixer fully to mix, be filled to complex pocket and namely obtain biological embryonic cell nutrient.
Described cell culture fluid composition and ratio is as follows: lactose 25g/L, caseinhydrolysate 4g/L, dusty yeast 3g/L, potassium dihydrogen phosphate 2g/L, ferrous lactate 2g/L, sodium selenite 0.03g/L, vitamin B6 0.03g/L/, vitamin C 8g/L, and surplus is water.
Described linseed is Canadian Field Park Company natural flax seed.
Embodiment 3
A kind of biological embryonic cell nutrient, is characterized in that: with linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son for raw material, obtains through biological cell culture technique; Wherein, with parts by weight, linseed 30 parts, broccoli seeds 10 parts, Semen sesami nigrum 10 parts, oat seed 10 parts, strange refined sub 10 parts.
The concrete preparation process of described biological cell culture technique is:
(1) linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son carried out respectively screen, clean, removal of impurities;
(2) take linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son in proportion, put into cell culture fluid respectively, rotating and culturing 5 days at 30 DEG C of temperature, rotary rpm is 150rpm; Cell culture fluid weight is respectively 2:1 with the ratio of linseed, broccoli seeds, Semen sesami nigrum, oat seed, each weight of strange refined son.
(3) linseed after step (2) being cultivated, broccoli seeds, Semen sesami nigrum, oat seed, the vacuum drying at 20-50 DEG C respectively of strange refined son, make its water content reach 3%-10%;
(4) step (3) dried linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are used biological cell wall breaking technology ultramicro grinding respectively, make its sporoderm-broken rate reach more than 99%, grain fineness reaches 4 μm;
(5) linseed crushed in step (4), broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are put into mixer fully to mix, be filled to complex pocket and namely obtain biological embryonic cell nutrient.
Described cell culture fluid composition and ratio is as follows: lactose 20g/L, caseinhydrolysate 3.5g/L, dusty yeast 2g/L, potassium dihydrogen phosphate 0.5g/L, ferrous lactate 3g/L, sodium selenite 1.0g/L, vitamin B6 0.05g/L/, Catergen 0g/L, and surplus is water.
Described linseed is Canadian Field Park Company natural flax seed.
Embodiment 4
A kind of biological embryonic cell nutrient, is characterized in that: with linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son for raw material, obtains through biological cell culture technique; Wherein, with parts by weight, linseed 70 parts, broccoli seeds 60 parts, Semen sesami nigrum 50 parts, oat seed 50 parts, strange refined sub 50 parts.
The concrete preparation process of described biological cell culture technique is:
(1) linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son carried out respectively screen, clean, removal of impurities;
(2) take linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son in proportion, put into cell culture fluid respectively, rotating and culturing 6 days at 25 DEG C of temperature, rotary rpm is 100rpm; Cell culture fluid weight is respectively 4:1 with the ratio of linseed, broccoli seeds, Semen sesami nigrum, oat seed, each weight of strange refined son.
(3) linseed after step (2) being cultivated, broccoli seeds, Semen sesami nigrum, oat seed, the vacuum drying at 20-50 DEG C respectively of strange refined son, make its water content reach 3%-10%;
(4) step (3) dried linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are used biological cell wall breaking technology ultramicro grinding respectively, make its sporoderm-broken rate reach more than 99%, grain fineness reaches 3.5 μm;
(5) linseed crushed in step (4), broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are put into mixer fully to mix, be filled to complex pocket and namely obtain biological embryonic cell nutrient.
Described cell culture fluid composition and ratio is as follows: lactose 40g/L, caseinhydrolysate 1g/L, dusty yeast 6g/L, potassium dihydrogen phosphate 1.5g/L, ferrous lactate 1g/L, sodium selenite 0.01g/L, vitamin B6 1.0g/L/, vitamin C 16g/L, and surplus is water.
Described linseed is Canadian Field Park Company natural flax seed.
Embodiment 5
A kind of biological embryonic cell nutrient, is characterized in that: with linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son for raw material, obtains through biological cell culture technique; Wherein, with parts by weight, linseed 50 parts, broccoli seeds 35 parts, Semen sesami nigrum 30 parts, oat seed 20 parts, strange refined sub 20 parts.
The concrete preparation process of described biological cell culture technique is:
(1) linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son carried out respectively screen, clean, removal of impurities;
(2) take linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son in proportion, put into cell culture fluid respectively, rotating and culturing 4 days at 35 DEG C of temperature, rotary rpm is 120rpm; Cell culture fluid weight is respectively 5:1 with the ratio of linseed, broccoli seeds, Semen sesami nigrum, oat seed, each weight of strange refined son.
(3) linseed after step (2) being cultivated, broccoli seeds, Semen sesami nigrum, oat seed, the vacuum drying at 20-50 DEG C respectively of strange refined son, make its water content reach 3%-10%;
(4) step (3) dried linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are used biological cell wall breaking technology ultramicro grinding respectively, make its sporoderm-broken rate reach more than 99%, grain fineness reaches 4.5 μm;
(5) linseed crushed in step (4), broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are put into mixer fully to mix, be filled to complex pocket and namely obtain biological embryonic cell nutrient.
Described cell culture fluid composition and ratio is as follows: lactose 30g/L, caseinhydrolysate 2.5g/L, dusty yeast 5g/L, potassium dihydrogen phosphate 2.5g/L, ferrous lactate 4g/L, sodium selenite 0.05g/L, vitamin B6 0.01g/L/, vitamin C 5g/L, and surplus is water.
Described linseed is Canadian Field Park Company natural flax seed.
Experimental example 1
Zoopery: the biological embryonic cell nutrient using preparation in embodiment 1, tests the effect that delays senility of this product.
Drosophila survival experiment
1. animal used as test: Drosophila melanogaster
2. method:
By fly blow 25 DEG C, 65% relative humidities hatches, the adult sprouted wings in 10 hours is anaesthetized with ether fume, male and female are distinguished under anatomical lens, 1600 are selected to be in charge of cultivation, often pipe 50, then 4 groups are divided at random, often organize 400, male and female half and half, wherein blank group is fed with basal medium, other three groups is experimental group, in basal medium, add 0.2%, 1%, 5% biological embryonic cell nutrient respectively, every day more renews culture medium, observes fruit bat male 52 days, female 60 days survival rates, and calculate average life span and maximum life span.
3. result: refer to table l.
Table 1: drosophila survival is tested
* compare P<0.05** with control group and compare P<0.01 with control group
Through statistical analysis, 0.2% experimental group compares P<0.05 with control group notable difference, and 1% has significant differences with 5% experimental group P<0.01 compared with control group.Show that biological embryonic cell nutrition have the effect of the life quality that significantly delays senility, improves.
Experimental example 2
This product takes 2 times every day, and each 20-30g takes after mixing it with water with warm water edible.
With 100 suffer from that insomnia and dreamful sleep, refreshing tired body are tired, neurasthenia, in poor health, etc. symptom for subjects, adopt and take this product for each person every day 2 times, each 20-30g, take 60 days, in experimenter, 70%-80% patient takes effect obviously or cures, 10%-20% patient takes effect generally, and 1%-10% patients goes out to take a turn for the better.
Above disclosedly be only preferred embodiment of the present invention, certainly can not limit the interest field of the present invention with this, therefore with any amendment, equivalent variations, improvement etc. that the claims in the present invention are done, the scope that the present invention is contained all should be included in.
Claims (7)
1. a biological embryonic cell nutrient, is characterized in that: with linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son for raw material, obtains through biological cell culture technique; Wherein, with parts by weight, linseed 30-70 part, broccoli seeds 10-60 part, Semen sesami nigrum 10-50 part, oat seed 10-50 part, strange refined sub-10-50 part;
The concrete preparation process of described biological cell culture technique is:
(1) linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son carried out respectively screen, clean, removal of impurities;
(2) take linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son in proportion, put into cell culture fluid respectively, rotating and culturing 3-7 days at 20-40 DEG C of temperature;
(3) linseed after step (2) being cultivated, broccoli seeds, Semen sesami nigrum, oat seed, the vacuum drying at 20-50 DEG C respectively of strange refined son, make its water content reach 3%-10%;
(4) step (3) dried linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are used biological cell wall breaking technology ultramicro grinding respectively, make its sporoderm-broken rate reach more than 99%;
(5) linseed crushed in step (4), broccoli seeds, Semen sesami nigrum, oat seed, strange refined son are put into mixer fully to mix, be filled to complex pocket and namely obtain biological embryonic cell nutrient.
2. the biological embryonic cell nutrient of one according to claim 1, is characterized in that: with parts by weight, linseed 30-60 part, broccoli seeds 20-50 part, Semen sesami nigrum 20-40 part, oat seed 20-40 part, strange refined sub-20-40 part.
3. the biological embryonic cell nutrient of one according to claim 1, it is characterized in that: described cell culture fluid composition and ratio is as follows: lactose 20-40g/L, caseinhydrolysate 1-4g/L, dusty yeast 2-6g/L, potassium dihydrogen phosphate 0.5-4g/L, ferrous lactate 1-5g/L, sodium selenite 0.01-1.0g/L, vitamin B6 0.01-1.0g/L/, vitamin C 5-20g/L, surplus is water.
4. the biological embryonic cell nutrient of one according to claim 1, is characterized in that: the rotary rpm in step (2) is 50-200rpm.
5. the biological embryonic cell nutrient of one according to claim 1, is characterized in that: in step (2), and cell culture fluid weight is respectively 2-5:1 with the ratio of linseed, broccoli seeds, Semen sesami nigrum, oat seed, each weight of strange refined son.
6. the biological embryonic cell nutrient of one according to claim 1, is characterized in that: in step (4), after breaking-wall cell ultramicro grinding, the grain fineness of linseed, broccoli seeds, Semen sesami nigrum, oat seed, strange refined son reaches 3-5 μm.
7. the biological embryonic cell nutrient of one according to claim 1, is characterized in that: described linseed is Canadian Field Park Company natural flax seed.
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