CN104430609A - Botanic pesticide water dispersant, preparation method and application thereof - Google Patents

Botanic pesticide water dispersant, preparation method and application thereof Download PDF

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Publication number
CN104430609A
CN104430609A CN201410663623.7A CN201410663623A CN104430609A CN 104430609 A CN104430609 A CN 104430609A CN 201410663623 A CN201410663623 A CN 201410663623A CN 104430609 A CN104430609 A CN 104430609A
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mass percentage
botanical pesticide
water dispersant
pesticide water
dispersant
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龙惊惊
李旭
王远
沈丽红
徐亚男
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Shenyang Research Institute of Chemical Industry Co Ltd
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Shenyang Research Institute of Chemical Industry Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention relates to the technical field of biological pesticides, and specifically discloses a botanic pesticide water dispersant, a preparation method and application of the water dispersant. The botanic pesticide water dispersant comprises a botanic pesticide, a plant growth regulator and an auxiliary including a filler and the like; by taking the mass percentage of the botanic pesticide water dispersant as the standard, the mass percentage of the botanic pesticide is 8-35%, and the mass percentage of the plant growth regulator is 2-5%. The botanic pesticide water dispersant can be taken as a pesticide for killing insects, especially lepidoptera pests.

Description

Botanical pesticide water dispersant, Preparation Method And The Use
Technical field
The present invention relates to biological pesticide technical field, particularly a kind of botanical pesticide water dispersant, Preparation Method And The Use.
Background technology
Present stage, China also exists the problem of serious chemical pesticide improper use.Excessive Pesticide use causes serious harm to the healthy of people and environment.For ensureing the quality safety of agricultural product, country advocates the Pesticide use of efficient, safety, environmental protection, low toxicity, low-residual energetically.
In addition, existing pesticide species is usually more single, and easily make insect develop immunity to drugs, drug effect is not good.Have some pesticide species preparation method technique comparatively complicated, cost is higher, is unsuitable for being applied to suitability for industrialized production.Therefore, a kind of drug effect of exploitation is needed badly better, the novel agrochemical that production cost is lower.
Summary of the invention
The present invention is intended to overcome agricultural chemicals of the prior art can destroy ecotope, easily allows insect produce the technological deficiency of drug resistance, provides a kind of novel plant source pesticide water dispersant, can be used for desinsection, in particular for killing lepidoptera pest as insecticide.While desinsection, can also promote the growth of plant, be the novel environment friendly agricultural chemicals of the fertile one of a kind of medicine.
For achieving the above object, the present invention is by the following technical solutions:
On the one hand, the invention provides a kind of botanical pesticide water dispersant, comprising: botanical pesticide, plant growth regulator and auxiliary agent, described auxiliary agent comprises filler; With the mass percentage of described botanical pesticide water dispersant for benchmark, the mass percentage of described botanical pesticide is 8% ~ 35%, and the mass percentage of described plant growth regulator is 2% ~ 5%.
Preferably, described filler is selected from one or more in kaolin, talcum powder, bentonite, attapulgite or white carbon.
Preferably, described botanical pesticide comprises datura flower, Chinese ash, giant knotweed, sweet wormwood and clausenae Lansium; With the mass percentage of described botanical pesticide water dispersant for benchmark, the mass percentage of described datura flower is 2% ~ 10%, the mass percentage of described Chinese ash is 1% ~ 5%, the mass percentage of described giant knotweed is 2% ~ 10%, the mass percentage of described sweet wormwood is 2% ~ 5%, and the mass percentage of described clausenae Lansium is 1% ~ 5%.
Preferably, described plant growth regulator comprises marine algae extract and bacillus subtilis polypeptide; With the mass percentage of described botanical pesticide water dispersant for benchmark, the mass percentage of described marine algae extract is 1% ~ 2%, and the mass percentage of described bacillus subtilis polypeptide is 1% ~ 3%.
Preferably, the amino acid sequence of described producing bacillus subtilis thing polypeptide is:
Asn-Asp-Met-Gln-Tyr-Asn-Gln-Asn-Met-Ser。
Preferably, the preparation method of described marine algae extract is: get the algae liquid that artificial seawater cultivates chrysophyceae, collected after centrifugation lower floor frond, as the concentrate of chrysophyceae; In the concentrate of chrysophyceae, add distilled water, ultrasonic extraction after adjustment pH=5, obtains extract; Extract is concentrated into dry.
Preferably, described auxiliary agent also comprises wetting agent, dispersant, disintegrant and binding agent.
Preferably, with the mass percentage of described botanical pesticide water dispersant for benchmark, the mass percentage of described wetting agent is 2% ~ 10%, the mass percentage of described dispersant is 1% ~ 20%, the mass percentage of described disintegrant is 1% ~ 10%, the mass percentage of described binding agent is 1% ~ 30%, and the mass percentage of described filler is surplus.
On the other hand, the invention provides a kind of preparation method of botanical pesticide water dispersant, by botanical pesticide, plant growth regulator and auxiliary agent Homogeneous phase mixing, after pulverizing, granulation, drying, screening, obtain botanical pesticide water dispersant.
On the other hand, the present invention also provides a kind of purposes of botanical pesticide water dispersant, is used for desinsection as insecticide.In particular for killing lepidoptera pest.
Beneficial effect of the present invention is: botanical pesticide water dispersant of the present invention take botanical pesticide as primary raw material, add appropriate dispersant, wetting agent, disintegrant, binding agent and filler again, again said mixture is pulverized, add water after mediating and carry out granulation, wet product particle again drying, screening obtains botanical pesticide water dispersant.The botanical pesticide water dispersant low toxicity prepared, pollution-free, comparatively safe to environment, people, animal, safety and environmental protection; Meanwhile, rapid disintegration in water, dispersion higher suspension and stable dispersion can be formed, easy to use, cost is low; In addition, botanical pesticide water dispersant provided by the invention, insect not easily develops immunity to drugs, and the lasting period is long, drug effect is high, while desinsection, can also promote the growth of plant, is the novel environment friendly agricultural chemicals of the fertile one of a kind of medicine.Botanical pesticide water dispersant preparation technology of the present invention is simple, and production cost is low, can be applicable to suitability for industrialized production.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with specific embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, and be not construed as limiting the invention.
The invention provides a kind of botanical pesticide water dispersant, comprising: botanical pesticide, plant growth regulator and auxiliary agent, auxiliary agent comprises filler; With the mass percentage of botanical pesticide water dispersant for benchmark, the mass percentage of botanical pesticide is 8% ~ 35%, and the mass percentage of plant growth regulator is 2% ~ 5%.
In concrete embodiment, botanical pesticide comprises datura flower, Chinese ash, giant knotweed, sweet wormwood and clausenae Lansium; With the mass percentage of botanical pesticide water dispersant for benchmark, the mass percentage of datura flower is 2% ~ 10%, the mass percentage of Chinese ash is 1% ~ 5%, the mass percentage of giant knotweed is 2% ~ 10%, the mass percentage of sweet wormwood is 2% ~ 5%, and the mass percentage of clausenae Lansium is 1% ~ 5%.Certainly, according to actual needs, botanical pesticide also can comprise other components again.
The preparation method of botanical pesticide comprises: screen the various crude drug datura flowers of botanical pesticide raw material, Chinese ash, giant knotweed, sweet wormwood and clausenae Lansium, reject damage by worms, go mouldy, impurity etc., the medicinal material chosen is cut into slices or is crushed to 20-40 order particle; (2) by each raw material by weight ratio, put into 10-15 times of industrial alcohol, at room temperature soak 72h, filter, after clarification, namely obtain botanical pesticide stoste; (3) botanical pesticide stoste is concentrated into dry, obtains botanical pesticide.
Plant growth regulator comprises marine algae extract and bacillus subtilis polypeptide; With the mass percentage of botanical pesticide water dispersant for benchmark, the mass percentage of marine algae extract is 1% ~ 2%, and the mass percentage of bacillus subtilis polypeptide is 1% ~ 3%.
Marine algae extract preferably adopts following methods to be prepared: get the algae liquid that artificial seawater cultivates chrysophyceae, collected after centrifugation lower floor frond, as the concentrate of chrysophyceae; In the concentrate of chrysophyceae, add distilled water, ultrasonic extraction after adjustment pH=5, obtains extract; Extract is concentrated into dry, obtains marine algae extract.
In embodiment, get artificial seawater and cultivate the algae liquid of chrysophyceae when carrying out centrifugal, centrifugal condition is preferably carried out centrifugal with the centrifugal speed of 6000rmp/min.When adding distilled water in the concentrate of chrysophyceae, the concentrate of chrysophyceae and the volume ratio of distilled water are preferably 1:10.When regulating pH=5, citric acid-sodium citrate buffer is preferably adopted to regulate.When ultrasonic extraction obtains extract, the condition of ultrasonic extraction is preferably in 200w, 30 DEG C of ultrasonic extraction 45min.When extract is concentrated into dry, concentrated condition be preferably in 60 DEG C ,-0.09mPa concentrates.
The amino acid sequence of bacillus subtilis polypeptide is: Asn-Asp-Met-Gln-Tyr-Asn-Gln-Asn-Met-Ser, 10 cyclic peptide.The bacterial strain producing this polypeptide is bacillus subtilis (Baciliius subtilis) bacterial strain, is a kind of endogenetic bacteria with desinsection and plant growth regulation obtained from kuh-seng root.
The concrete preparation process of bacillus subtilis polypeptide is as follows:
1, polypeptide crude extract is prepared
Adopt liquid nutrient medium aseptic fermentation to obtain zymotic fluid, the fermentation culture conditions of concrete zymotic fluid is: be 50mL at the liquid amount of 250mL triangular flask, and with in the inoculum concentration access culture fluid of 20%, the rotating speed of shaking table is 150rmin -1, cultivation temperature 28 DEG C, the pH of initial incubation liquid is 9.0, fermented and cultured 36h.Medium consists of glucose 2.0%, dusty yeast 0.5%, soybean meal 0.5%, NH 4nO 30.5%, CaCO 31.0%.
Get the zymotic fluid of 100mL bacillus subtilis B36 bacterial strain, 400mL absolute ethyl alcohol, be placed in 4 DEG C of refrigerator precooling 1h, both are mixed rear overnight at room temperature precipitation, filtrate is poured out, then carries out suction filtration, filtrate reduced in volume, 5mL is settled to, obtained polypeptide crude extract with deionized water.
2, polypeptide crude extract purifying
Polypeptide crude extract obtained in step 1 adopts silica gel column chromatography to be further purified, and specifically comprises the following steps:
(1) pretreatment of silica gel: get 200-300 order silica gel, use 1.0molL -1hCL soak 12h, remove the acid soluble material in silica gel, then use 80% alcohol immersion 12h, remove the liposoluble substance in silica gel, by process after silica gel in 110 DEG C, activate 24h.
(2) dry column-packing is adopted: by the even and disposable 3.5 × 50cm lower end of pouring into of silica gel after activation with in the chromatographic column of sieve plate, Zhuan Zhu limit, limit is knocked with rubber pipette bulb, after dress post, continue to knock chromatography post jamb with rubber pipette bulb again, knock up and down and make silica gel depositing homogeneous, strike the quartz sand adding 0.5cm in fact, ensure that sample elution height is consistent.
With 1500mL benzinum: the pre-drip washing silicagel column of mixed liquor of ethyl acetate=1:1, being to wash away its impurity on the one hand, being to make the abundant depositing homogeneous of silica gel on the other hand, finally making drip washing liquid level higher than silicagel column 1-2cm, close lower end valve, for subsequent use.
(3) loading: 30g polypeptide crude extract (thick) glue head dropper to drip in chromatographic column on quartz sand, ensure the lower edge as far as possible level of sample in the silica gel of column top as far as possible, otherwise cause wash-out to intersect, after infiltering quartz sand under sample is whole, add eluant, eluent carry out gradient elution.
(4) following solvent gradient elution is adopted: (1) 3000mL benzinum: ethyl acetate=1:1; (2) 3000mL benzinum: ethyl acetate=1:2; (3) 5000mL benzinum: ethyl acetate=1:5 can pressurize to accelerate elution speed on application of sample bottle, receives eluent with ground triangular flask below chromatographic column, collects benzinum: ethyl acetate=1:5 part eluent.Eluent is concentrated into dry, obtains the bacillus subtilis polypeptide after purifying.
Wherein, the preparation method of bacillus subtilis B36 bacterial strain is open in non-patent literature, specifically refer to Agricultural University Of Shenyang's thesis for the doctorate of 2009, Article Titles is the screening of kuh-seng Endophytic antagonistic bacteria and antibacterial material Purification thereof, and Authors of Science Articles is Wang Fang.Those skilled in the art can obtain B36 bacterial strain used in the application according to content disclosed in the document, and the applicant of the application ensured to provide this biomaterial to the public in Two decades years from the applying date.
In botanical pesticide water dispersant provided by the invention, filler is selected from one or more in kaolin, talcum powder, bentonite, attapulgite or white carbon.
Auxiliary agent, except filler, also comprises wetting agent, dispersant, disintegrant and binding agent.
With the mass percentage of botanical pesticide water dispersant for benchmark, wherein, the mass percentage of wetting agent is 2% ~ 10%, the mass percentage of dispersant is 1% ~ 20%, the mass percentage of disintegrant is 1% ~ 10%, the mass percentage of binding agent is 1% ~ 30%, and the mass percentage of filler is surplus.
Preferred embodiment, dispersant be selected from lignosulfonates, naphthalenesulfonic acid-formaldehyde condensate, polycarboxylate, alkylphenol formaldehyde condensate sulfate, to one or more in methoxyl group fatty acyl amido benzene sulfonic acid sodium salt, sodium lignin sulfonate, calcium lignosulfonate, polyoxyethylene poly-oxygen propylene aether block copolymer.
Wetting agent is selected from one or more in phenethyl phenol polyethenoxy ether condensation product, alkylphenol polyoxyethylene formaldehyde condensation products, alkyl benzene calcium sulfonate, alkyl benzene calcium sulfonate, phenethyl phenol polyethenoxy ether sodium sulfate salt or sulphate.Wherein, sulphate can be various types of sulphate such as alkyl sulfate.
Disintegrant is selected from one or more in calcium chloride, magnesium chloride, ammonium sulfate, urea.
Binding agent is selected from one or more in starch, dextrin, gelatin, polyvinyl alcohol, polyvinylpyrrolidone, polyethylene glycol or carboxymethyl cellulose.
In addition, present invention also offers the preparation method of above-mentioned botanical pesticide water dispersant, comprising: by botanical pesticide, plant growth regulator and auxiliary agent Homogeneous phase mixing, after pulverizing, granulation, drying, screening, obtain botanical pesticide water dispersant.
In embodiment, preparation method comprises: by weight 8% ~ 35% botanical pesticide, 2 ~ 5% plant growth regulator, 1 ~ 20% dispersant, 2 ~ 10% wetting agents, 1 ~ 10% disintegrant, 1 ~ 30% binding agent and uniform filling being mixed, pulverize again, add water after mediating and carry out granulation, wet product particle again drying, screening obtains described botanical pesticide water dispersant.
Botanical pesticide water dispersant provided by the invention take botanical pesticide as primary raw material, add appropriate plant growth regulator, dispersant, wetting agent, disintegrant, binding agent and filler again, again said mixture is pulverized, add water after mediating and carry out granulation, wet product particle again drying, screening obtains botanical pesticide water dispersant.The botanical pesticide water dispersant low toxicity prepared, pollution-free, comparatively safe to environment, people, animal, safety and environmental protection; Meanwhile, rapid disintegration in water, dispersion higher suspension and stable dispersion can be formed, easy to use, cost is low; In addition, botanical pesticide water dispersant provided by the invention, insect not easily develops immunity to drugs, and the lasting period is long, drug effect is high, while desinsection, can also promote the growth of plant, is the novel environment friendly agricultural chemicals of the fertile one of a kind of medicine.Botanical pesticide water dispersant preparation technology of the present invention is simple, and production cost is low, can be applicable to suitability for industrialized production.
In addition, present invention also offers above-mentioned botanical pesticide water dispersant and be applied to desinsection as insecticide.
Preferred embodiment, above-mentioned botanical pesticide water dispersant is applied to and kills lepidoptera pest.
Set forth the present invention further below by way of specific embodiment, these embodiments are only presented for purposes of illustration, do not limit the scope of the invention.
Embodiment 1
Get datura flower 2% respectively, Chinese ash 2%, giant knotweed 2%; sweet wormwood 1%, clausenae Lansium 1%, marine algae extract 1%; bacillus subtilis polypeptide 1%; phenethyl phenol polyethenoxy ether condensation product 2%, calcium chloride 2%, starch 1%; kaolin is mended and is mixed after 100%; air-flow crushing, adds water after mediating, proceeds in the comminutor that certain specification screen cloth is housed and carry out granulation.Wet product particle again drying, sieve and obtain water dispersible granules.This botanical pesticide water dispersible granules is without caking, energy free-flow, and well, heat-storage is stablized for wetability and quality suspensibility.
Embodiment 2
Get datura flower 10% respectively, Chinese ash 10%, giant knotweed 10%; sweet wormwood 5%, clausenae Lansium 5%, marine algae extract 2%; bacillus subtilis polypeptide 3%, alkylphenol polyoxyethylene formaldehyde condensation products 2%, naphthalenesulfonic acid-formaldehyde condensate 1%;, magnesium chloride 2%, dextrin 1%; talcum powder mixes after complementing to 100%; air-flow crushing, adds water after mediating, proceeds in the comminutor that certain specification screen cloth is housed and carry out granulation.Wet product particle again drying, sieve and obtain water dispersible granules.This botanical pesticide water dispersible granules is without caking, energy free-flow, and well, heat-storage is stablized for wetability and quality suspensibility.
Embodiment 3
Get datura flower 5% respectively, Chinese ash 5%, giant knotweed 6%; sweet wormwood 5%, clausenae Lansium 5%, marine algae extract 1%; bacillus subtilis polypeptide 2%, alkyl benzene calcium sulfonate 2%, polycarboxylate 2%; ammonium sulfate 2%; gelatin 8%, mixes after white carbon complements to 100%, air-flow crushing; add water after mediating, proceed in the comminutor that certain specification screen cloth is housed and carry out granulation.Wet product particle again drying, sieve and obtain water dispersible granules.This botanical pesticide water dispersible granules is without caking, energy free-flow, and well, heat-storage is stablized for wetability and quality suspensibility.
Embodiment 4
Get datura flower 5% respectively, Chinese ash 2%, giant knotweed 5%; sweet wormwood 2%, clausenae Lansium 5%, marine algae extract 2%; bacillus subtilis polypeptide 1%, alkyl sulfate 2%, alkylphenol formaldehyde condensate sulfate 1%; urea 1%; polyvinyl alcohol 1%, mixes after attapulgite complements to 100%, air-flow crushing; add water after mediating, proceed in the comminutor that certain specification screen cloth is housed and carry out granulation.Wet product particle again drying, sieve and obtain water dispersible granules.This botanical pesticide water dispersible granules is without caking, energy free-flow, and well, heat-storage is stablized for wetability and quality suspensibility.
Embodiment 5
Get datura flower 5% respectively, Chinese ash 8%, giant knotweed 5%; sweet wormwood 5%, clausenae Lansium 5%, marine algae extract 1%; bacillus subtilis polypeptide 1%, marine algae extract 10%, alkyl benzene calcium sulfonate 10%; to methoxyl group fatty acyl amido benzene sulfonic acid sodium salt 20%, calcium chloride 10%, gelatin 30%; white carbon mixes after complementing to 100%; air-flow crushing, adds water after mediating, proceeds in the comminutor that certain specification screen cloth is housed and carry out granulation.Wet product particle again drying, sieve and obtain water dispersible granules.This botanical pesticide water dispersible granules is without caking, energy free-flow, and well, heat-storage is stablized for wetability and quality suspensibility.
Embodiment 6
Get datura flower 2% respectively, Chinese ash 2%, giant knotweed 2%; sweet wormwood 5%, clausenae Lansium 5%, marine algae extract 2%; bacillus subtilis polypeptide 2%; alkyl benzene calcium sulfonate 2%, polyoxyethylene poly-oxygen propylene aether block copolymer 3%, polyvinylpyrrolidone 2%; white carbon mixes after complementing to 100%; air-flow crushing, adds water after mediating, proceeds in the comminutor that certain specification screen cloth is housed and carry out granulation.Wet product particle again drying, sieve and obtain water dispersible granules.This botanical pesticide water dispersible granules is without caking, energy free-flow, and well, heat-storage is stablized for wetability and quality suspensibility.
Performance test
1, botanical pesticide water dispersant is on the impact of plant strain growth
After spraying wheat plant leaf surface with the botanical pesticide water dispersant 50ppm in the bacillus subtilis polypeptide containing 200 μ g polypeptide/ml and embodiment 1, the biostimulation activity of bacillus subtilis polypeptide is assessed.In contrast, with water spray be in the identical developmental stage and under same environmental conditions the plant of incubation.Calculate the average plant height of 20 strain plant, root length and root fresh weight, result is as shown in table 1, can find out, the plant strain growth spraying the botanical pesticide water dispersant in bacillus subtilis polypeptide and embodiment 1 is better.
Table 1 botanical pesticide water dispersant is on the impact of plant strain growth
2, the mensuration of botanical pesticide water dispersant insecticidal effect
Artificial feeding class medicine embrane method is adopted to carry out lepidopterous larvae insecticidal activity assay.Basic operation is as follows: enough 24 well culture plates preparing every hole 1ml artificial feed, add 500mg/L liquid on artificial feed surface and form medicine film, every hole adds amount of liquid medicine 0.05mL, worm is connect after liquid dries, every Kong Yitou tries worm, each concentration is a process, often processes 1 culture plate totally 24 examination worms.Observation ward is put into, the death condition of routine observation examination worm after being disposed.Check after 7d and record the dead head number trying worm, calculating lethality.
Investigate the insecticidal effect of the botanical pesticide water dispersant of embodiment 1-6 respectively, measure target lethality (%), result is as shown in table 2.
The experimental result of table 2 embodiment 1-6
As can be seen from Table 2, the botanical pesticide water dispersant prepared of embodiment 1-6 to diamond-back moth and mythimna separata insect killing effect remarkable.And these botanical pesticide water dispersants can be degraded under field conditions (factors), reduce the pesticide resistance of insect.
The above the specific embodiment of the present invention, does not form limiting the scope of the present invention.Any various other done by technical conceive of the present invention change and distortion accordingly, all should be included in the protection domain of the claims in the present invention.

Claims (10)

1. a botanical pesticide water dispersant, is characterized in that, comprising: botanical pesticide, plant growth regulator and auxiliary agent, and described auxiliary agent comprises filler;
With the mass percentage of described botanical pesticide water dispersant for benchmark, the mass percentage of described botanical pesticide is 8% ~ 35%, and the mass percentage of described plant growth regulator is 2% ~ 5%.
2. botanical pesticide water dispersant as claimed in claim 1, it is characterized in that, described filler is selected from one or more in kaolin, talcum powder, bentonite, attapulgite or white carbon.
3. botanical pesticide water dispersant as claimed in claim 1, it is characterized in that, described botanical pesticide comprises datura flower, Chinese ash, giant knotweed, sweet wormwood and clausenae Lansium; With the mass percentage of described botanical pesticide water dispersant for benchmark, the mass percentage of described datura flower is 2% ~ 10%, the mass percentage of described Chinese ash is 1% ~ 5%, the mass percentage of described giant knotweed is 2% ~ 10%, the mass percentage of described sweet wormwood is 2% ~ 5%, and the mass percentage of described clausenae Lansium is 1% ~ 5%.
4. botanical pesticide water dispersant as claimed in claim 1, it is characterized in that, described plant growth regulator comprises marine algae extract and bacillus subtilis polypeptide; With the mass percentage of described botanical pesticide water dispersant for benchmark, the mass percentage of described marine algae extract is 1% ~ 2%, and the mass percentage of described bacillus subtilis polypeptide is 1% ~ 3%.
5. botanical pesticide water dispersant as claimed in claim 4, it is characterized in that, the amino acid sequence of described producing bacillus subtilis thing polypeptide is:
Asn-Asp-Met-Gln-Tyr-Asn-Gln-Asn-Met-Ser。
6. botanical pesticide water dispersant as claimed in claim 4, it is characterized in that, the preparation method of described marine algae extract is: get the algae liquid that artificial seawater cultivates chrysophyceae, collected after centrifugation lower floor frond, as the concentrate of chrysophyceae; In the concentrate of chrysophyceae, add distilled water, ultrasonic extraction after adjustment pH=5, obtains extract; Extract is concentrated into dry.
7. botanical pesticide water dispersant as claimed in claim 1, it is characterized in that, described auxiliary agent also comprises wetting agent, dispersant, disintegrant and binding agent.
8. botanical pesticide water dispersant as claimed in claim 7, it is characterized in that, with the mass percentage of described botanical pesticide water dispersant for benchmark, the mass percentage of described wetting agent is 2% ~ 10%, the mass percentage of described dispersant is 1% ~ 20%, the mass percentage of described disintegrant is 1% ~ 10%, and the mass percentage of described binding agent is 1% ~ 30%, and the mass percentage of described filler is surplus.
9. the preparation method of botanical pesticide water dispersant as claimed in claim 1, it is characterized in that, described preparation method comprises: by botanical pesticide, plant growth regulator and auxiliary agent Homogeneous phase mixing, after pulverizing, granulation, drying, screening, obtain botanical pesticide water dispersant.
10. the purposes of botanical pesticide water dispersant as claimed in claim 1, is characterized in that, is used for desinsection as insecticide.
CN201410663623.7A 2014-11-19 2014-11-19 Botanic pesticide water dispersant, preparation method and application thereof Pending CN104430609A (en)

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王芳: "《苦参内生拮抗细菌筛选及其抗菌物质纯化鉴定》", 《沈阳农业大学博士学位论文》 *
许加超: "《海藻化学与工艺学》", 30 September 2014, 中国海洋大学出版社 *

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