CN104398601B - A kind of pharmaceutical composition treating insomnia and preparation method thereof - Google Patents
A kind of pharmaceutical composition treating insomnia and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 title claims abstract description 8
- 206010022437 insomnia Diseases 0.000 title claims abstract description 8
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 5
- 239000000284 extract Substances 0.000 claims abstract description 83
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 79
- 238000010992 reflux Methods 0.000 claims abstract description 48
- 239000003208 petroleum Substances 0.000 claims abstract description 27
- 239000012259 ether extract Substances 0.000 claims abstract description 16
- -1 filters Substances 0.000 claims abstract description 13
- 239000000706 filtrate Substances 0.000 claims abstract description 12
- 239000003814 drug Substances 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 239000002671 adjuvant Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000012567 medical material Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 230000002557 soporific effect Effects 0.000 claims description 5
- 230000002936 tranquilizing effect Effects 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims description 2
- 239000000047 product Substances 0.000 claims description 2
- 238000009795 derivation Methods 0.000 abstract description 2
- 239000012467 final product Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 241000699670 Mus sp. Species 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000002481 ethanol extraction Methods 0.000 description 5
- 239000002024 ethyl acetate extract Substances 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 230000002567 autonomic effect Effects 0.000 description 4
- 230000006837 decompression Effects 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 230000004799 sedative–hypnotic effect Effects 0.000 description 4
- 241000205571 Caulophyllum Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 230000028527 righting reflex Effects 0.000 description 3
- 230000004622 sleep time Effects 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 206010003084 Areflexia Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000003326 hypnotic agent Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 206010006100 Bradykinesia Diseases 0.000 description 1
- 241000218208 Caltha Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000006479 Cyme Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 208000006083 Hypokinesia Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 206010041349 Somnolence Diseases 0.000 description 1
- 229940049706 benzodiazepine Drugs 0.000 description 1
- 150000001557 benzodiazepines Chemical class 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007665 chronic toxicity Effects 0.000 description 1
- 231100000160 chronic toxicity Toxicity 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- AAOVKJBEBIDNHE-UHFFFAOYSA-N diazepam Chemical class N=1CC(=O)N(C)C2=CC=C(Cl)C=C2C=1C1=CC=CC=C1 AAOVKJBEBIDNHE-UHFFFAOYSA-N 0.000 description 1
- 229940084926 diazepam 2 mg Drugs 0.000 description 1
- 208000010643 digestive system disease Diseases 0.000 description 1
- 206010013663 drug dependence Diseases 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 208000018685 gastrointestinal system disease Diseases 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000000147 hypnotic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 229940012022 pentobarbital sodium 50 mg Drugs 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 238000009702 powder compression Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 239000000932 sedative agent Substances 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- 208000019116 sleep disease Diseases 0.000 description 1
- 230000003860 sleep quality Effects 0.000 description 1
- 208000020685 sleep-wake disease Diseases 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 208000011117 substance-related disease Diseases 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of pharmaceutical composition treating insomnia and preparation method, this medicine by Herba Calthae extract and pharmaceutically can adjuvant be prepared from, the described ether extract that Herba Calthae extract is Herba Calthae；Preparation method is: by Herba Calthae through petroleum ether reflux, extract, filters, and filtrate discards, and gained filtering residue extracts through aether backflow, to obtain final product.The present invention can not only shorten time for falling asleep, i.e. shortens the sleep derivation phase, and can extend the length of one's sleep, and deepens Depth of sleep.
Description
Technical field
The invention belongs to active components of plants extractive technique field, in particular to a kind of drug regimen treating insomnia
Thing and preparation method thereof.
Background technology
Insomnia is one of clinical modal symptom, be sleep originate and maintain generation obstacle, sleep quality can not meet
Human Physiology needs, thus affects normal activity on daytime, occurs the one of the symptoms such as fatigue, aprosexia, bradykinesia
Plant sleep disorder syndrome.Modern life rhythm is increasingly faster, and many people have the immense pressure from various aspects, make
Obtain ortho sleep can not be guaranteed, have groups of people cannot fall asleep because pressure is huge.Often insomnia causes attention not collect
In, understandability declines, the learning efficiency reduces, hypomnesis, thus affect the duty of people, have a sleepless night for a long time
Also can reduce the immunity of body, cause endocrine disturbance, easily induce and increase the weight of gastrointestinal disease, infectious disease, the heart
Cerebrovascular disease etc..For alleviating these pressure, the Benzodiazepines such as insomniac often relies on hypnotic drug and falls asleep, diazepam
Class sedative hypnotic, but these medicines help fall asleep while also bring a lot of side effect, as additive, drug resistance,
Chronic toxicity.And, continuous application easily produces giddy, the untoward reaction such as drowsiness, weak.There are some researches show many
Chinese herbal medicine has good sedative-hypnotic effect, and untoward reaction is less, without additive, therefore, seeks from natural plants
Look for new sedative hypnotic drug to seem to be even more important.
Herba Calthae is the herb of cohosh kincup, belongs to perennial herb, high 40～60 centimetres；Rhizome is sturdy,
Crawl.Stem purple, hollow, normal branch；Basal leaf oval or heart, diameter 5～12 centimetres, edge tool rough sawn tooth,
Base portion is the most heart-shaped, and two sides is without hair；Petiole length 6～15 centimetres, purple green；Stem leaf and basal leaf similar shape, have stipule,
The leaf tool handle of bottom, upper leaf stockless.Uniparous cyme is born in stem or branch top, brightly yellowish color, diameter 1.6～3.2
Centimetre；Sepal 5, petal-shaped, ovum shape is oval, is about 1～1.8 centimetre；Without petal；Stamen is most, flower pesticide yellow；
Carpel number piece is from life；Really oblong, is about 1 centimetre, and tip becomes hook-shaped, green.Seed is most, and the kidney shape is long
Ellipse, there is longitudinal grin on surface, and brown is glossy；Florescence 4～May；Really phase 6～July.At present, there is no document report
Herba Calthae extract has effect of tranquilizing soporific.
Summary of the invention
It is an object of the invention to provide a kind of tranquilizing soporific effect notable, untoward reaction is less, without addicted medicine group
Compound and its preparation method and application.
In order to realize the purpose of the present invention, inventor, by lot of experiments research persistent exploration, is finally obtained following skill
Art scheme:
A kind of pharmaceutical composition treating insomnia, by Herba Calthae extract and pharmaceutically can adjuvant be prepared from, described
The ether extract that Herba Calthae extract is Herba Calthae.It should be noted that according to the common process of formulation art, than
Such as direct powder compression, wet granule compression tablet method etc., those skilled in the art is easy to gained Herba Calthae extract
The adjuvant available with on pharmaceutics is prepared as the oral formulations commonly used, such as granule, tablet, capsule, pill, oral
Liquid etc..Wherein, available on pharmaceutics adjuvant includes filler, disintegrating agent, binding agent, correctives, lubricant.
Present invention also offers the preparation method of described Herba Calthae extract, the method comprises the steps: to weigh Herba Calthae
Medical material, loads in reflux, extract, device, adds petroleum ether, put reflux, extract, 1-5 time under 70-80 DEG C of water bath with thermostatic control environment,
0.5-2.5h every time, filters, and filtrate discards after reclaiming petroleum ether, and filtering residue, after volatilization removes petroleum ether, loads backflow and carries
During fetching is put, add ether, put reflux, extract, 1-5 time under 38-45 DEG C of water bath with thermostatic control environment, each 0.5-2.5h, filter,
Concentrating filter liquor is dried obtains Herba Calthae ether extract.When the present invention prepares Herba Calthae ether extract, selected medicine
Material is the herb before cohosh kincup blooms.
Preferably, the preparation method of Herba Calthae extract as above, wherein the addition volume of petroleum ether is quality of medicinal material
5-15 times (L/kg), more preferably 7-8 times (L/kg).
Preferably, the preparation method of Herba Calthae extract as above, wherein the volume that adds of ether is quality of medicinal material
6-12 times (L/kg), more preferably 7-8 times (L/kg).
Preferably, the preparation method of Herba Calthae extract as above, wherein before each reflux, extract, medical material is soaked
5-60min, more preferably 30-40min.
Preferably, the preparation method of Herba Calthae extract as above, the number of times that wherein petroleum ether or aether backflow are extracted
For 2-4 time, extraction time successively decreases successively.
In several embodiments on ground preferred for this invention, the preparation method of Herba Calthae extract as above, Qi Zhongshi
Oil ether or aether backflow extract number of times be 3 times, the 1st reflux, extract, l.0-1.5h, the 2nd reflux, extract, 0.7-1.0h,
3rd reflux, extract, 0.4-0.6h.
The present invention uses organic solvent to screen Herba Calthae sedative-hypnotic effect position, by a large amount of animal experiment tables
Bright, Herba Calthae ether extract prepared by the present invention is the longest to the prolonged sleep time of mice, other solvent extractable matter effects
More weak, may be relatively fewer relevant with this extract part amount without effective ingredient or contained effective ingredient.Therefore, this
Another bright purpose is to provide a kind of pharmaceutical applications, it may be assumed that above-mentioned Herba Calthae extract is used for tranquilizing soporific in preparation
Medicine or health product in application.
It is an advantage of the invention that the effect of tranquilizing soporific is notable, time for falling asleep can not only be shortened, i.e. shorten the sleep derivation phase,
And the length of one's sleep can be extended, and deepen Depth of sleep.It addition, the medicine of the present invention is without obvious toxic-side effects, through long-term
Use does not results in drug dependence, and raw material sources are extensive simultaneously, and preparation technology is simple, it is not necessary to main equipment, produces into
This is low.
Detailed description of the invention
The following is preparation embodiment and the effect test example of each effective extracting section thing dry extract of Herba Calthae of the present invention, to this
Bright technical scheme and technique effect are described further and explain.When the present invention prepares Herba Calthae ether extract, selected
Medical material be the herb before cohosh kincup blooms.
Embodiment 1: the preparation of Herba Calthae ether extract
Accurately weigh dry Herba Calthae medical material 0.5kg, chopping, load in 6L round-bottomed flask, with 1:8 (kg:L)
Solid-liquid ratio add petroleum ether, soak after 30min, put reflux, extract, 3 times in 75 DEG C of thermostat water baths, return for the 1st time
Stream extracts lh, the 2nd reflux, extract, 45min, the 3rd reflux, extract, 30min, filters, after filtrate reclaims petroleum ether
Discarding, filtering residue, after volatilization removes petroleum ether, loads in 6L round-bottomed flask, adds with the solid-liquid ratio of 1:8 (kg:L)
Enter ether, after soaking 30min, put reflux, extract, 3 times in 40 DEG C of thermostat water baths, the 1st reflux, extract, 1h, the
2 reflux, extract, 45min, the 3rd reflux, extract, 30min, to filter, merge 3 extracting solution, filtrate is rotating steaming
Sending out decompression and solvent recovery on instrument, concentrated solution obtains dark brown dry extract, is weighed as 27.53g after volatilizing solvent, yield is
5.51%.
Embodiment 2: the preparation of Herba Calthae ether extract
Accurately weigh dry Herba Calthae medical material 1.0kg, chopping, load in 10L round-bottomed flask, with 1:7 (kg:L)
Solid-liquid ratio add petroleum ether, soak after 45min, put reflux, extract, 3 times in 75 DEG C of thermostat water baths, return for the 1st time
L.5h stream extract, the 2nd reflux, extract, 1h, the 3rd reflux, extract, 30min, filters, and filtrate is abandoned after reclaiming petroleum ether
Going, filtering residue, after volatilization removes petroleum ether, loads in 6L round-bottomed flask, adds by the solid-liquid ratio of 1:7 (kg:L)
Ether, soaks after 45min, puts reflux, extract, 3 times in 40 DEG C of thermostat water baths, the 1st reflux, extract, 1.5h, and the 2nd
Secondary reflux, extract, 1h, the 3rd reflux, extract, 30min, to filter, merge 3 extracting solution, filtrate is on a rotary evaporator
Decompression and solvent recovery, concentrated solution obtains dark brown dry extract, is weighed as 53.70g after volatilizing solvent, yield is 5.37%.
Comparative example 1: the preparation of Herba Calthae ethanol extraction
Accurately weigh dry Herba Calthae medical material 0.5kg, chopping, load in 6L round-bottomed flask, with 1:8 (kg:L)
Solid-liquid ratio add petroleum ether, soak after 30min, put reflux, extract, 3 times in 75 DEG C of thermostat water baths, return for the 1st time
Stream extracts lh, the 2nd reflux, extract, 45min, the 3rd reflux, extract, 30min, filters, after filtrate reclaims petroleum ether
Discarding, filtering residue, after volatilization removes petroleum ether, loads in 6L round-bottomed flask, adds by the solid-liquid ratio of 1:8 (kg:L)
Enter ethanol, after soaking 30min, put reflux, extract, 3 times in 90 DEG C of thermostat water baths, the 1st reflux, extract, 1h, the
2 reflux, extract, 45min, the 3rd reflux, extract, 30min, to filter, merge 3 extracting solution, filtrate is rotating steaming
Sending out decompression and solvent recovery on instrument, concentrated solution obtains dark brown dry extract, is weighed as 60.18g after volatilizing solvent, yield is
12.04%.
Comparative example 2: the preparation of Herba Calthae ethyl acetate extract
Accurately weigh dry Herba Calthae medical material 0.5kg, chopping, load in 6L round-bottomed flask, with 1:8 (kg:L)
Solid-liquid ratio add petroleum ether, soak after 30min, put reflux, extract, 3 times in 75 DEG C of thermostat water baths, return for the 1st time
Stream extracts lh, the 2nd reflux, extract, 45min, the 3rd reflux, extract, 30min, filters, after filtrate reclaims petroleum ether
Discarding, filtering residue, after volatilization removes petroleum ether, loads in 6L round-bottomed flask, adds with the solid-liquid ratio of 1:8 (kg:L)
Enter ethyl acetate, after soaking 30min, put reflux, extract, 3 times in 85 DEG C of thermostat water baths, the 1st reflux, extract, 1h,
2nd reflux, extract, 45min, the 3rd reflux, extract, 30min, to filter, merge 3 extracting solution, filtrate is rotating
Decompression and solvent recovery on evaporimeter, concentrated solution obtains dark brown dry extract, is weighed as 31.44g after volatilizing solvent, yield is
6.29%.
The experimental study to Sedative And Hypnotic Effect of the embodiment 3 Herba Calthae extract
Cleaning grade Kunming mouse 50, male and female half and half, body weight (20 ± 2) g.It is randomly divided into blank group, positive control
Group, ether extract group, ethanol extraction group, ethyl acetate extract group, often 10 animals of group, male and female half and half.Second
Ether extract group gives the Herba Calthae ether extract 40.0mg/kg of the embodiment of the present invention 1 preparation, and ethanol extraction group gives
The Herba Calthae ethanol extraction 40.0mg/kg of above-mentioned comparative example 1 preparation, ethyl acetate extract group gives above-mentioned contrast
The Herba Calthae ethyl acetate extract 40.0mg/kg of embodiment 2 preparation, positive controls gives diazepam 2mg/kg, blank
Matched group gives isopyknic normal saline；The equal gastric infusion of each group, every day 1 time, continuous 3 days, gavage volume 15ml/kg.
Stand number of times and the activity time of 5min after putting into case with mice autonomic activities instrument record mice after being administered 60min in second day
Number.After last is administered 60min, each group mice equal lumbar injection pentobarbital sodium 50mg/kg, sleep disappears with righting reflex
Losing is index, keeps more than 30s person with the downward posture of animal back, it is judged that for righting reflex loss, record injection penta bar ratio
To mice sleeping duration as dropping asleep latency after appropriate sodium, and observed and recorded righting reflex loss to awakening time
Between as the prolonged sleep time.
By the result of the test of table 1 it can be seen that except ether extraction beyond the region of objective existence, the solvent extractable matter of other Herba Calthaes is to normally
Mice autonomic activities, almost without impact, improves the frequency of mice autonomic activities the most to a certain extent；It addition, holding
Continuing the aspect length of one's sleep, ether extract, ethanol extraction and ethyl acetate extract group compare the brightest with blank group
Aobvious prolongation, but only ether extract group is the most notable on the impact of prolonged sleep time, compares with blank group and has
Pole significant difference (P < 0.01), compares with positive controls and has significant difference (P < 0.05), and ether carries simultaneously
Take thing group and shorten dropping asleep latency, compare with blank group or positive controls and be respectively provided with significant difference
(P<0.05)。
Table 1 is respectively organized mice autonomic activities and compares the length of one's sleep
Compare with blank group,★P ＜ 0.05,★★P ＜ 0.01；Compare with positive controls,▼P ＜ 0.05,▼▼P ＜ 0.01.
Claims (8)
1. the pharmaceutical composition treating insomnia, by Herba Calthae extract and pharmaceutically can adjuvant be prepared from, it is characterized in that, the described ether extract that Herba Calthae extract is Herba Calthae, prepare as follows: weigh Herba Calthae medical material, load in reflux, extract, device, add petroleum ether, put reflux, extract, 1-5 time under 70-80 DEG C of water bath with thermostatic control environment, 0.5-2.5h every time, filter, filtrate discards after reclaiming petroleum ether, filtering residue is after volatilization removes petroleum ether, load in reflux, extract, device, add ether, put reflux, extract, 1-5 time under 38-45 DEG C of water bath with thermostatic control environment, 0.5-2.5h every time, filter, concentrating filter liquor is dried obtains Herba Calthae ether extract.
The preparation method of Herba Calthae extract the most according to claim 1, it is characterized in that, the method comprises the steps: to weigh Herba Calthae medical material, load in reflux, extract, device, add petroleum ether, put reflux, extract, 1-5 time under 70-80 DEG C of water bath with thermostatic control environment, 0.5-2.5h every time, filter, filtrate discards after reclaiming petroleum ether, filtering residue is after volatilization removes petroleum ether, load in reflux, extract, device, add ether, put reflux, extract, 1-5 time under 38-45 DEG C of water bath with thermostatic control environment, 0.5-2.5h every time, filter, concentrating filter liquor is dried obtains Herba Calthae ether extract.
The preparation method of Herba Calthae extract the most according to claim 2, it is characterised in that the volume that adds of petroleum ether is 5-15 times of quality of medicinal material.
The preparation method of Herba Calthae extract the most according to claim 2, it is characterised in that the volume that adds of ether is 6-12 times of quality of medicinal material.
The preparation method of Herba Calthae extract the most according to claim 2, it is characterised in that every time before reflux, extract, medical material is soaked 5-60min.
The preparation method of Herba Calthae extract the most according to claim 2, it is characterised in that the number of times that petroleum ether or aether backflow are extracted is 2-4 time, and extraction time successively decreases successively.
The preparation method of Herba Calthae extract the most according to claim 6, it is characterised in that the number of times that petroleum ether or aether backflow are extracted is 3 times, the 1st reflux, extract, l.0-1.5h, the 2nd reflux, extract, 0.7-1.0h, the 3rd reflux, extract, 0.4-0.6h.
8. the Herba Calthae extract described in claim 1 is in the application prepared in the medicine or health product of tranquilizing soporific.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410685210.9A CN104398601B (en) | 2014-11-25 | 2014-11-25 | A kind of pharmaceutical composition treating insomnia and preparation method thereof |
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Inventor after: Zhang Shoutao Inventor after: Ma Hui Inventor after: Lin Xutao Inventor after: Sun Huili Inventor after: Shang Yimei Inventor before: Ma Hui Inventor before: Zhang Shoutao Inventor before: Lin Xutao Inventor before: Zhang Zhenmei Inventor before: Zhao Jie |
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Effective date of registration: 20181214 Address after: 100016 Beijing Chaoyang District Jiuxianqiao East Road, No. 9 Courtyard, Building 3, 301, 30 Co-patentee after: Song Xuejun Patentee after: Secret Biography (Beijing) Technology Co., Ltd. Address before: 256603 No. 661 No. 2 Huanghe Road, Bincheng District, Binzhou City, Shandong Province Patentee before: Binzhou Medical University Hospital |
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