CN104398601A - Medicine composition for treating insomnia and preparation method for medicine composition - Google Patents
Medicine composition for treating insomnia and preparation method for medicine composition Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 239000003814 drug Substances 0.000 title claims abstract description 11
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 title claims abstract description 9
- 206010022437 insomnia Diseases 0.000 title claims abstract description 9
- 239000000203 mixture Substances 0.000 title abstract description 9
- 229940079593 drug Drugs 0.000 title description 2
- 239000000284 extract Substances 0.000 claims abstract description 78
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 70
- 238000010992 reflux Methods 0.000 claims abstract description 50
- 239000003208 petroleum Substances 0.000 claims abstract description 25
- 239000012259 ether extract Substances 0.000 claims abstract description 16
- 239000000706 filtrate Substances 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000000605 extraction Methods 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000012567 medical material Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 6
- -1 load in reflux Substances 0.000 claims description 5
- 239000008194 pharmaceutical composition Substances 0.000 claims description 5
- 230000002557 soporific effect Effects 0.000 claims description 5
- 230000002936 tranquilizing effect Effects 0.000 claims description 5
- 239000002671 adjuvant Substances 0.000 claims description 4
- 230000007423 decrease Effects 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 239000000047 product Substances 0.000 claims description 2
- 230000002035 prolonged effect Effects 0.000 abstract description 5
- 230000004622 sleep time Effects 0.000 abstract description 5
- 241001113320 Ligularia fischeri Species 0.000 abstract 4
- 230000006698 induction Effects 0.000 abstract 1
- 239000002904 solvent Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 238000002791 soaking Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 238000002481 ethanol extraction Methods 0.000 description 5
- 239000002024 ethyl acetate extract Substances 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 230000002567 autonomic effect Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 230000006837 decompression Effects 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 230000004799 sedative–hypnotic effect Effects 0.000 description 4
- 206010003084 Areflexia Diseases 0.000 description 3
- 241000205571 Caulophyllum Species 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000028527 righting reflex Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000003326 hypnotic agent Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 206010006100 Bradykinesia Diseases 0.000 description 1
- 241000218208 Caltha Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000006479 Cyme Species 0.000 description 1
- 206010057315 Daydreaming Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 208000006083 Hypokinesia Diseases 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 1
- 241000220259 Raphanus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 206010041349 Somnolence Diseases 0.000 description 1
- 229940049706 benzodiazepine Drugs 0.000 description 1
- 150000001557 benzodiazepines Chemical class 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007665 chronic toxicity Effects 0.000 description 1
- 231100000160 chronic toxicity Toxicity 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- AAOVKJBEBIDNHE-UHFFFAOYSA-N diazepam Chemical compound N=1CC(=O)N(C)C2=CC=C(Cl)C=C2C=1C1=CC=CC=C1 AAOVKJBEBIDNHE-UHFFFAOYSA-N 0.000 description 1
- 229960003529 diazepam Drugs 0.000 description 1
- 229940084926 diazepam 2 mg Drugs 0.000 description 1
- 208000010643 digestive system disease Diseases 0.000 description 1
- 206010013663 drug dependence Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 208000018685 gastrointestinal system disease Diseases 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000000147 hypnotic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 229940012022 pentobarbital sodium 50 mg Drugs 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 238000009702 powder compression Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 239000000932 sedative agent Substances 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- 208000019116 sleep disease Diseases 0.000 description 1
- 230000003860 sleep quality Effects 0.000 description 1
- 208000020685 sleep-wake disease Diseases 0.000 description 1
- 208000011117 substance-related disease Diseases 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a medicine composition for treating insomnia and a preparation method for the medicine composition. The medicine composition is prepared from a ligularia fischeri extract and pharmaceutically available auxiliary materials, wherein the ligularia fischeri extract is ether extract of ligularia fischeri. The preparation method comprises the following steps: performing reflux extraction on ligularia fischeri by using petroleum ether, filtering, discarding the filtrate, and performing reflux extraction on the obtained filter residue by using ether to obtain the medicine composition. By adopting the medicine composition, not only is the fall-asleep time shortened, that is, the sleep induction period is shortened, but also the sleep time is prolonged, and the sleep depth is increased.
Description
Technical field
The invention belongs to active components of plants extractive technique field, in particular to a kind of pharmaceutical composition and preparation method thereof of Cure for insomnia.
Background technology
Insomnia is one of clinical modal symptom; starting and maintaining generation obstacle of sleep; sleep quality can not meet Human Physiology needs, thus affects normal activity on daytime, occurs a kind of sleep disorder syndrome of the symptoms such as fatigue, aprosexia, bradykinesia.Modern life rhythm is more and more faster, and many people have the immense pressure from various aspects, and ortho sleep be can not be guaranteed, and have groups of people cannot fall asleep because pressure is huge.Frequent insomnia causes that absent minded, understandability declines, the learning efficiency reduces, hypomnesis, thus affect the duty of people, long-time insomnia also can reduce the immunity of body, cause endocrine disturbance, easily bring out and increase the weight of gastrointestinal disease, infectious disease, cardiovascular and cerebrovascular disease etc.For alleviating these pressure, insomniac often relies on hypnotic drug to fall asleep, the Benzodiazepines sedative hypnotic such as diazepam, but these medicines also bring a lot of side effect, as additive, drug resistance, chronic toxicity while help is fallen asleep.And continuous application easily produces giddy, the untoward reaction such as drowsiness, weak.There are some researches show that many Chinese herbal medicine have good sedative-hypnotic effect, and untoward reaction is less, without additive, therefore, from natural plants, finds new sedative hypnotic drug seem and be even more important.
Herba Calthae is the herb of cohosh kincup, belongs to perennial herb, high 40 ~ 60 centimetres; Rhizome is sturdy, crawls.Stem purple, hollow, normal branch; Basal leaf oval or heart, diameter 5 ~ 12 centimetres, edge tool rough sawn tooth, base portion is deeply heart-shaped, and two sides is without hair; Long 6 ~ 15 centimetres of petiole, purple green; Stem leaf and basal leaf similar shape, tool stipule, the leaf tool handle of bottom, upper leaf stockless.Uniparous cyme is born in stem or branch top, brightly yellowish color, diameter 1.6 ~ 3.2 centimetres; Sepal 5, petal-shaped, ovum shape is oval, is about 1 ~ 1.8 centimetre; Without petal; Stamen is most, and flower pesticide is yellow; Carpel number is piece from life; Follicle radish fruit oblong, be about 1 centimetre, tip becomes hook-shaped, green.Seed is most, kidney shape oblong, and there is longitudinal grin on surface, and brown is glossy; 4 ~ May of florescence; Really 6 ~ July of phase.At present, there is no effect that bibliographical information Herba Calthae extract has tranquilizing soporific.
Summary of the invention
The object of the present invention is to provide a kind of tranquilizing soporific Be very effective, untoward reaction is less, without addicted pharmaceutical composition and its preparation method and application.
In order to realize object of the present invention, inventor, by lot of experiments research also persistent exploration, finally obtains following technical scheme:
A pharmaceutical composition for Cure for insomnia, by Herba Calthae extract and pharmaceutically can adjuvant be prepared from, described Herba Calthae extract is the ether extract of Herba Calthae.It should be noted that, according to the common process of formulation art, such as direct powder compression, wet granule compression tablet method etc., those skilled in the art is easy to adjuvant available on gained Herba Calthae extract and pharmaceutics is prepared into conventional oral formulations, as granule, tablet, capsule, pill, oral liquid etc.Wherein, available on pharmaceutics adjuvant comprises filler, disintegrating agent, binding agent, correctives, lubricant.
Present invention also offers the preparation method of described Herba Calthae extract, the method comprises the steps: to take Herba Calthae medical material, load in reflux, extract, device, add petroleum ether, to put under 70-80 DEG C of water bath with thermostatic control environment reflux, extract, 1-5 time, each 0.5-2.5h, filter, filtrate discards after reclaiming petroleum ether, and filtering residue is after volatilization removing petroleum ether, load in reflux, extract, device, add ether, to put under 38-45 DEG C of water bath with thermostatic control environment reflux, extract, 1-5 time, each 0.5-2.5h, filter, after concentrating filter liquor drying, obtain Herba Calthae ether extract.When the present invention prepares Herba Calthae ether extract, selected medical material is the herb before cohosh kincup blooms.
Preferably, the preparation method of Herba Calthae extract as above, wherein petroleum ether add volume be the 5-15 of quality of medicinal material doubly (L/kg), more preferably 7-8 is doubly (L/kg).
Preferably, the preparation method of Herba Calthae extract as above, wherein ether add volume be the 6-12 of quality of medicinal material doubly (L/kg), more preferably 7-8 is doubly (L/kg).
Preferably, the preparation method of Herba Calthae extract as above, wherein soaks 5-60min by medical material, more preferably 30-40min before each reflux, extract.
Preferably, the preparation method of Herba Calthae extract as above, the number of times that wherein petroleum ether or aether backflow are extracted is 2-4 time, and extraction time successively decreases successively.
In several embodiments on ground preferred for this invention, the preparation method of Herba Calthae extract as above, the number of times that wherein petroleum ether or aether backflow are extracted is 3 times, and the 1st reflux, extract, is l.0-1.5h, 2nd reflux, extract, 0.7-1.0h, the 3rd reflux, extract, 0.4-0.6h.
The present invention adopts organic solvent to screen Herba Calthae sedative-hypnotic effect position, shown by a large amount of animal experiment, Herba Calthae ether extract prepared by the present invention prolonged sleep time to mice is the longest, other solvent extractable matter effects are more weak, may not contain the relatively rare pass of amount of effective ingredient or contained effective ingredient by extract part therewith.Therefore, another object of the present invention is to provide a kind of pharmaceutical applications, that is: above-mentioned Herba Calthae extract is for the preparation of the application in the medicine of tranquilizing soporific or health product.
Advantage of the present invention is the Be very effective of tranquilizing soporific, can not only shorten time for falling asleep, namely shortens the sleep derivation phase, and can extend the length of one's sleep, and deepen Depth of sleep.In addition, medicine of the present invention, without obvious toxic-side effects, can not cause drug dependence through life-time service, and raw material sources are extensive simultaneously, and preparation technology is simple, and without the need to main equipment, production cost is low.
Detailed description of the invention
Be below preparation embodiment and the effect test example of Herba Calthae of the present invention each live part extract dry extract, technical scheme of the present invention and technique effect are described further and are explained.When the present invention prepares Herba Calthae ether extract, selected medical material is the herb before cohosh kincup blooms.
Embodiment 1: the preparation of Herba Calthae ether extract
Accurately take dry Herba Calthae medical material 0.5kg, chopping, load in 6L round-bottomed flask, petroleum ether is added with the solid-liquid ratio of 1:8 (kg:L), after soaking 30min, to put in 75 DEG C of thermostat water baths reflux, extract, 3 times, 1st reflux, extract, lh, 2nd reflux, extract, 45min, 3rd reflux, extract, 30min, filter, filtrate discards after reclaiming petroleum ether, filtering residue is after volatilization removing petroleum ether, load in 6L round-bottomed flask, ether is added with the solid-liquid ratio of 1:8 (kg:L), after soaking 30min, to put in 40 DEG C of thermostat water baths reflux, extract, 3 times, 1st reflux, extract, 1h, 2nd reflux, extract, 45min, 3rd reflux, extract, 30min, filter, merge 3 extracting solution, filtrate is decompression and solvent recovery on a rotary evaporator, concentrated solution obtains dark brown dry extract after volatilizing solvent, be weighed as 27.53g, yield is 5.51%.
Embodiment 2: the preparation of Herba Calthae ether extract
Accurately take dry Herba Calthae medical material 1.0kg, chopping, load in 10L round-bottomed flask, petroleum ether is added with the solid-liquid ratio of 1:7 (kg:L), after soaking 45min, to put in 75 DEG C of thermostat water baths reflux, extract, 3 times, 1st reflux, extract, l.5h, 2nd reflux, extract, 1h, 3rd reflux, extract, 30min, filter, filtrate discards after reclaiming petroleum ether, filtering residue is after volatilization removing petroleum ether, load in 6L round-bottomed flask, ether is added by the solid-liquid ratio of 1:7 (kg:L), after soaking 45min, to put in 40 DEG C of thermostat water baths reflux, extract, 3 times, 1st reflux, extract, 1.5h, 2nd reflux, extract, 1h, 3rd reflux, extract, 30min, filter, merge 3 extracting solution, filtrate is decompression and solvent recovery on a rotary evaporator, concentrated solution obtains dark brown dry extract after volatilizing solvent, be weighed as 53.70g, yield is 5.37%.
Comparative example 1: the preparation of Herba Calthae ethanol extraction
Accurately take dry Herba Calthae medical material 0.5kg, chopping, load in 6L round-bottomed flask, petroleum ether is added with the solid-liquid ratio of 1:8 (kg:L), after soaking 30min, to put in 75 DEG C of thermostat water baths reflux, extract, 3 times, 1st reflux, extract, lh, 2nd reflux, extract, 45min, 3rd reflux, extract, 30min, filter, filtrate discards after reclaiming petroleum ether, filtering residue is after volatilization removing petroleum ether, load in 6L round-bottomed flask, ethanol is added by the solid-liquid ratio of 1:8 (kg:L), after soaking 30min, to put in 90 DEG C of thermostat water baths reflux, extract, 3 times, 1st reflux, extract, 1h, 2nd reflux, extract, 45min, 3rd reflux, extract, 30min, filter, merge 3 extracting solution, filtrate is decompression and solvent recovery on a rotary evaporator, concentrated solution obtains dark brown dry extract after volatilizing solvent, be weighed as 60.18g, yield is 12.04%.
Comparative example 2: the preparation of Herba Calthae ethyl acetate extract
Accurately take dry Herba Calthae medical material 0.5kg, chopping, load in 6L round-bottomed flask, petroleum ether is added with the solid-liquid ratio of 1:8 (kg:L), after soaking 30min, to put in 75 DEG C of thermostat water baths reflux, extract, 3 times, 1st reflux, extract, lh, 2nd reflux, extract, 45min, 3rd reflux, extract, 30min, filter, filtrate discards after reclaiming petroleum ether, filtering residue is after volatilization removing petroleum ether, load in 6L round-bottomed flask, ethyl acetate is added with the solid-liquid ratio of 1:8 (kg:L), after soaking 30min, to put in 85 DEG C of thermostat water baths reflux, extract, 3 times, 1st reflux, extract, 1h, 2nd reflux, extract, 45min, 3rd reflux, extract, 30min, filter, merge 3 extracting solution, filtrate is decompression and solvent recovery on a rotary evaporator, concentrated solution obtains dark brown dry extract after volatilizing solvent, be weighed as 31.44g, yield is 6.29%.
Embodiment 3 Herba Calthae extract is to the experimental study of Sedative And Hypnotic Effect
Cleaning grade Kunming mouse 50, male and female half and half, body weight (20 ± 2) g.Be divided into blank group, positive controls, ether extract group, ethanol extraction group, ethyl acetate extract group at random, often organize 10 animals, male and female half and half.Ether extract group gives Herba Calthae ether extract 40.0mg/kg prepared by the embodiment of the present invention 1, ethanol extraction group gives above-mentioned comparative example 1 the Herba Calthae ethanol extraction 40.0mg/kg of preparation, ethyl acetate extract group gives above-mentioned comparative example 2 the Herba Calthae ethyl acetate extract 40.0mg/kg of preparation, positive controls gives diazepam 2mg/kg, and blank group gives isopyknic normal saline; Each group of equal gastric infusion, every day 1 time, continuous 3 days, gavage volume 15ml/kg.Stand number of times and the movable number of times of 5min put into case with mice autonomic activities instrument record mice after second day administration 60min after.After last administration 60min, each group of mice equal lumbar injection pentobarbital sodium 50mg/kg, sleep is index with righting reflex loss, more than 30s person is kept with the downward posture of animal back, be judged as righting reflex loss, record injection pentobarbital sodium after to mice sleeping duration as dropping asleep latency, and observed and recorded righting reflex loss to awakening time as the prolonged sleep time.
Can be found out by the result of the test of table 1, except ether extraction beyond the region of objective existence, the solvent extractable matter of other Herba Calthaes almost without impact, improves the frequency of mice autonomic activities on normal mouse autonomic activities even to a certain extent, in addition, in the prolonged sleep time, ether extract, ethanol extraction and ethyl acetate extract group more obviously extend with blank group, but only have ether extract group the most remarkable on the impact of prolonged sleep time, compare with blank group and there is pole significant difference (P<0.01), compare with positive controls and there is significant difference (P<0.05), ether extract group shortens dropping asleep latency simultaneously, with blank group or positive controls, more all there is significant difference (P<0.05).
Table 1 is respectively organized mice autonomic activities and is compared the length of one's sleep
Compare with blank group,
★p < 0.05,
★ ★p < 0.01; Compare with positive controls,
▼p < 0.05,
▼ ▼p < 0.01.
Claims (8)
1. a pharmaceutical composition for Cure for insomnia, by Herba Calthae extract and pharmaceutically can adjuvant be prepared from, it is characterized in that, described Herba Calthae extract is the ether extract of Herba Calthae.
2. the preparation method of Herba Calthae extract according to claim 1, it is characterized in that, the method comprises the steps: to take Herba Calthae medical material, load in reflux, extract, device, add petroleum ether, to put under 70-80 DEG C of water bath with thermostatic control environment reflux, extract, 1-5 time, each 0.5-2.5h, filter, filtrate discards after reclaiming petroleum ether, filtering residue is after volatilization removing petroleum ether, load in reflux, extract, device, add ether, to put under 38-45 DEG C of water bath with thermostatic control environment reflux, extract, 1-5 time, each 0.5-2.5h, filter, Herba Calthae ether extract is obtained after concentrating filter liquor drying.
3. the preparation method of Herba Calthae extract according to claim 2, is characterized in that, petroleum ether add volume be the 5-15 of quality of medicinal material doubly.
4. the preparation method of Herba Calthae extract according to claim 2, is characterized in that, ether add volume be the 6-12 of quality of medicinal material doubly.
5. the preparation method of Herba Calthae extract according to claim 2, is characterized in that, before each reflux, extract, medical material is soaked 5-60min.
6. the preparation method of Herba Calthae extract according to claim 2, is characterized in that, the number of times that petroleum ether or aether backflow are extracted is 2-4 time, and extraction time successively decreases successively.
7. the preparation method of Herba Calthae extract according to claim 6, is characterized in that, the number of times that petroleum ether or aether backflow are extracted is 3 times, the 1st reflux, extract, l.0-1.5h, the 2nd reflux, extract, 0.7-1.0h, the 3rd reflux, extract, 0.4-0.6h.
8. Herba Calthae extract according to claim 1 is for the preparation of the application in the medicine of tranquilizing soporific or health product.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102397503A (en) * | 2011-10-09 | 2012-04-04 | 邓华伦 | Chinese herbal medicine for treating insomnia |
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| CN102397503A (en) * | 2011-10-09 | 2012-04-04 | 邓华伦 | Chinese herbal medicine for treating insomnia |
Non-Patent Citations (3)
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| 云南省药物研究所编著: "《云南天然药物图鉴》", 31 May 2004, article "马鞍叶", pages: 73 * |
| 李经纬等主编: "《中医大辞典》", 31 May 1995, article "夜合叶根", pages: 970 * |
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| CN104398601B (en) | 2016-08-24 |
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Inventor after: Zhang Shoutao Inventor after: Ma Hui Inventor after: Lin Xutao Inventor after: Sun Huili Inventor after: Shang Yimei Inventor before: Ma Hui Inventor before: Zhang Shoutao Inventor before: Lin Xutao Inventor before: Zhang Zhenmei Inventor before: Zhao Jie |
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Effective date of registration: 20181214 Address after: 100016 Beijing Chaoyang District Jiuxianqiao East Road, No. 9 Courtyard, Building 3, 301, 30 Co-patentee after: Song Xuejun Patentee after: Secret Biography (Beijing) Technology Co., Ltd. Address before: 256603 No. 661 No. 2 Huanghe Road, Bincheng District, Binzhou City, Shandong Province Patentee before: Binzhou Medical University Hospital |
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