CN102120000B - Composition of effective parts of traditional Chinese medicine preparation for resisting Coxsackie virus B and preparation method thereof - Google Patents
Composition of effective parts of traditional Chinese medicine preparation for resisting Coxsackie virus B and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a composition of effective parts of a compound traditional Chinese medicine preparation and a preparation method thereof, in particular to a composition of effective parts of a traditional Chinese medicine preparation for resisting Coxsackie virus B and a preparation method thereof. The technical scheme is as follows: one or combination of a total polysaccharide part, a total flavone part and a total saponin part is contained; and the total polysaccharide part, the total flavone part and the total saponin part are respectively extracted from traditional Chinese medicine coarse powder according to three modes. The invention overcomes the defects that the existing capsule has large taking dosage and short action time and is inconvenient to store. All raw medicines are extracted and separated, and parts with highest medical effects are screened out and combined, thereby keeping the combined action of the medicine components in the composition, being favorable for exerting the multiple-effect property of traditional Chinese medicines, removing tissue substances and partial ineffective components, obviously improving the activity, reducing the dosage, enhancing the drug compliance and facilitating storage and transportation. The method provided by the invention has simple and safe processes and is suitable for industrialized production, and the prepared effective parts are active components which can be prepared into various pharmaceutical dosage forms.
Description
Technical field
The present invention relates to a kind of composition and method of making the same of effective site of Chinese traditional compound medicine, the composition and method of making the same of particularly anti-CBV Chinese medicine preparation effective site.
Background technology
Coxsackie virus is a kind of in the more common virus of nature, can often cause diseases such as respiratory tract infection, intestinal infection, aseptic meningitis, myocarditis, pericarditis through respiratory tract and digestive tract infection human body.Simultaneously, in utero Coxsackie virus infection also is one of high risk factor that brings out congenital heart disease.Children's and between twenty and fifty susceptible, especially infant during this century, Coxsackie virus was very popular for several times both at home and abroad, and involve heart more.Doctor trained in Western medicine does not have specific treatment to viral myocarditis, and the slow intravenous injection of general heavy dose of high concentration vitamin C promotes cardiomyopathy to recover.Commonly used for many years promotes that like polarized solution, Energy mixture and ATP etc. the energy of ischemic myocardial cells is synthetic, stablize the damaged myocardium cell membrane, improves myocardium pumping function, reduces the MDA generation, improves myocardial ischemia, alleviates the tissue injury due to the cardiac muscle.But, can not give full play to the effect of protection cardiac muscle all because of myocardial cell difficult to get access.Although multiple single medicinal material has the effect of anti-CB, what supply clinical use is the Chinese medicine crude drug at present, does not have the fixed prescription preparation, causes taking dose bigger, the medical material waste.Chinese invention patent: ZL
200310101631.4,Title " anti-CBV property myocarditis capsule and preparation method thereof " has solved the problems referred to above, and its Chinese medicine weight percentages of components is following: Radix Panacis Quinquefolii 15-30%, Semen Raphani 5-15%, Semen Vaccariae 4-10%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Cortex Moutan 10-25%, the Radix Astragali 30-40%.But there is following problem in background technology: Chinese medicine taking dosage is big, stores inconvenience, and the storage time is short.
Summary of the invention
The object of the invention provides a kind of composition and method of making the same of anti-CBV Chinese medicine preparation effective site; Both kept the comprehensive function of each medicinal ingredient in the compound recipe; Help bringing into play the pleiotropy of Chinese medicine, removed suck tissue and partial invalidity composition again, prolonged the storage life, improved purity, reduced dose; The repeatability of quality control improves, and solves the problems referred to above that background technology exists.
The present invention seeks to realize through following technical scheme:
A kind of compositions of anti-CBV Chinese medicine preparation effective site comprises one of total polysaccharides position, total flavone part, total saponins position or combination each other; Select Chinese medicine Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae, clean, be ground into coarse powder, can all sieve, but can be no more than 40% through the amount of No. four medicines sieves of 65 purposes through No. two medicines of 20 purposes; Preparation by weight percentage, the percentage by weight of each Chinese medicinal components is following: Radix Panacis Quinquefolii 30-40%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Radix Astragali 30-40%, Cortex Moutan 10-25%, Semen Raphani 5-15%, Semen Vaccariae 4-10%; The Chinese medicine coarse powder extracts total polysaccharides position, total flavone part, total saponins position respectively by following three kinds of modes;
1. extract the total polysaccharides position: the Chinese medicine coarse powder adds 6-10 and doubly measures the 90-95% soak with ethanol after 0.5-1 hour, and backflow 1-2 hour, 6-10 layer filtered through gauze, medicinal residues boil 2-4 time with 6-10 times of decocting, and each 2-4 hour, collecting decoction was concentrated into 1-0.5:1; Concentrated solution is contained alcohol with 90-95% ethanol accent measure to 60-80%, filter, the drying precipitated powder that obtains is the total polysaccharides position;
2. extract total flavone part: the Chinese medicine coarse powder adds 6-10 and doubly measures the 70-80% soak with ethanol after 0.5-1 hour, and backflow 2-4 hour, 10 layers of filtered through gauze of 6-, medicinal residues were with 4-8 80% alcohol reflux 2-3 time doubly, 2-4 hour at every turn.The rotary evaporation concentrated extracting solution is to 1-0.5:1, and with 3-6 defat of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with ethyl acetate equal-volume extraction 3-6 time, the rotary evaporation extract obtains powder after the drying, be total flavone part;
3. extract the total saponins position: the Chinese medicine coarse powder adds 6-10 and doubly measures the 70-80% soak with ethanol after 0.5-1 hour, and backflow 2-4 hour, 10 layers of filtered through gauze of 6-, medicinal residues were with 4-8 80% alcohol reflux 2-3 time doubly, 2-4 hour at every turn; The rotary evaporation concentrated extracting solution is to 1-0.5:1, and with 3-6 defat of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with water-saturated n-butanol equal-volume extraction 3-6 time, the rotary evaporation extract obtains powder after the drying, be the total saponins position.
The gained powder combinations becomes effective part group and suitable adjuvant to be prepared into clinical required dosage form use.
A kind of preparation method of composition of anti-CBV Chinese medicine preparation effective site; Comprise the steps: to select Chinese medicine Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae; Clean; Be ground into coarse powder, can all sieve, but can be no more than 40% through the amount of No. four medicines sieves of 65 purposes through No. two medicines of 20 purposes; Preparation by weight percentage, the percentage by weight of each Chinese medicinal components is following: Radix Panacis Quinquefolii 30-40%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Radix Astragali 30-40%, Cortex Moutan 10-25%, Semen Raphani 5-15%, Semen Vaccariae 4-10%; The Chinese medicine coarse powder extracts total polysaccharides position, total flavone part, total saponins position respectively by following three kinds of modes, one of total polysaccharides position, total flavone part, total saponins position or be combined as the compositions of anti-CBV Chinese medicine preparation effective site each other;
1. extract the total polysaccharides position: the Chinese medicine coarse powder adds 6-10 and doubly measures the 90-95% soak with ethanol after 0.5-1 hour, and backflow 1-2 hour, 6-10 layer filtered through gauze, medicinal residues boil 2-4 time with 6-10 times of decocting, and each 2-4 hour, collecting decoction was concentrated into 1-0.5:1; Concentrated solution is contained alcohol with 90-95% ethanol accent measure to 60-80%, filter, the drying precipitated powder that obtains is the total polysaccharides position;
2. extract total flavone part: the Chinese medicine coarse powder adds 6-10 and doubly measures the 70-80% soak with ethanol after 0.5-1 hour, and backflow 2-4 hour, 10 layers of filtered through gauze of 6-, medicinal residues were with 4-8 80% alcohol reflux 2-3 time doubly, 2-4 hour at every turn.The rotary evaporation concentrated extracting solution is to 1-0.5:1, and with 3-6 defat of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with ethyl acetate equal-volume extraction 3-6 time, the rotary evaporation extract obtains powder after the drying, be total flavone part;
3. extract the total saponins position: the Chinese medicine coarse powder adds 6-10 and doubly measures the 70-80% soak with ethanol after 0.5-1 hour, and backflow 2-4 hour, 10 layers of filtered through gauze of 6-, medicinal residues were with 4-8 80% alcohol reflux 2-3 time doubly, 2-4 hour at every turn; The rotary evaporation concentrated extracting solution is to 1-0.5:1, and with 3-6 defat of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with water-saturated n-butanol equal-volume extraction 3-6 time, the rotary evaporation extract obtains powder after the drying, be the total saponins position.
Preferred implementation:
Extract the total polysaccharides position: the Chinese medicine coarse powder added 8 times of amount 95% soak with ethanol after 0.5 hour, refluxed 1.5 hours, and 8 layers of filtered through gauze, medicinal residues boil 2 times with 8 times of decoctings, and each 2 hours, collecting decoction was concentrated into 0.5:1.Concentrated solution is contained the alcohol amount to 60% with 95% ethanol accent, filter the drying precipitated total polysaccharides position that obtains.
Extract total flavone part: Chinese medicine coarse powder Chinese medicine coarse powder added 8 times of amount 80% soak with ethanol after 0.5 hour, refluxed 2 hours, and 8 layers of filtered through gauze, medicinal residues were with 6 times of 80% alcohol reflux 2 times, each 2 hours; The rotary evaporation concentrated extracting solution is to 0.5:1, and with 3 defats of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with ethyl acetate equal-volume extraction 3 times, the rotary evaporation extract obtains total flavone part after the drying.
Extract the total saponins position: Chinese medicine coarse powder Chinese medicine coarse powder added 8 times of amount 80% soak with ethanol after 0.5 hour, refluxed 2 hours, and 8 layers of filtered through gauze, medicinal residues were with 6 times of 80% alcohol reflux 2 times, each 2 hours; The rotary evaporation concentrated extracting solution is to 0.5:1, and with 3 defats of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with water-saturated n-butanol equal-volume extraction 3 times, the rotary evaporation extract obtains the total saponins position after the drying.
The present invention can be prepared into the various dosage forms of anti-CBV with Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae compatibility, and the medicine utilization rate of active components is obviously improved.
Radix Panacis Quinquefolii is cold in nature, sweet and slightly bitter taste, people's lung, spleen channel.Have boosting qi and nourishing yin, the effect for promoting the production of body fluid is supported in the relieving restlessness of relieving inflammation or internal heat.
Fructus Schisandrae Chinensis sour in the mouth, sweet, warm in nature, return lung, the heart, kidney channel.It is astringent or styptic treatment for spontaneous sweating to have convergence, supplementing QI for promoting the production of body fluid, the effect of kidney calming.
Radix Ophiopogonis sweet and slightly bitter taste, cold nature, GUIXIN, lung, stomach warp.Has YIN nourishing and the production of body fluid promoting, the effect that lung moistening clears away heart-fire.
Radix Astragali sweet in the mouth, slightly warm in nature is returned lung, spleen, liver, kidney channel.Benefiting QI for strengthening the superficies is arranged, and arresting sweating takes off admittedly, holder skin ulcer granulation promoting, the effect of inducing diuresis to remove edema.
Cortex Moutan bitter in the mouth, suffering, cold nature, GUIXIN, liver, kidney channel.Has clearing away heat and cooling blood, the effect of promoting blood circulation to remove blood stasis.
Semen Raphani acrid in the mouth, sweet, property is flat.Return lung, spleen, stomach warp.Having and helping digestion except that expanding the effect of lowering the adverse-rising QI to resolve phlegm.
The Semen Vaccariae bitter in the mouth, property is flat.Return liver, the stomach warp.Promoting blood circulation to restore menstrual flow is arranged, stimulating milk secretion eliminating carbuncle, the effect of inducing diuresis for treating stranguria syndrome.
Can reduce the sensitivity of cardiac muscle to virus entirely, human body immunity improving power and anti-virus ability have heart tonifying, reduce effects such as oxygen consumes, correction arrhythmia.
The present invention has characteristics such as curative effect is clear and definite, high-efficiency low-toxicity, taking convenience, and anti-CBV effect is remarkable.
The present invention overcome existing capsule taking dose big, store inconvenience, short shortcoming of time, through to full side's raw medicinal material with the The suitable solvent extraction separation and filter out the high-order bit of drug effect and make up, both kept the comprehensive function of each medicinal ingredient in the compound recipe; Help bringing into play the pleiotropy of Chinese medicine, removed suck tissue and partial invalidity composition again, activity obviously improves; And can reduce dose; Strengthen drug compliance, the repeatability of quality control improves, convenient storage and transportation.Method technology of the present invention is simple and safe; It is low that gained effective site combination drug effect is better than former side's production cost; Be suitable for suitability for industrialized production, the effective site of preparing is that active component can be made into various pharmaceutical dosage forms, like soft capsule, tablet, drop pill, injection, controlled release agent or the like.
The specific embodiment
Below through embodiment the present invention is described further.
In an embodiment; Anti-CBV Chinese medicine preparation is selected Chinese medicine Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae; Clean, be ground into coarse powder (can all sieve, but can be no more than 40%) through the amount of No. four medicines sieves through No. two medicines; Preparation by weight percentage, the percentage by weight of each Chinese medicinal components is following: Radix Panacis Quinquefolii 30-40%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Radix Astragali 30-40%, Cortex Moutan 10-25%, Semen Raphani 5-15%, Semen Vaccariae 4-10%; After adding 8 times of amount 95% soak with ethanol 0.5h, backflow 1.5h, 8 layers of filtered through gauze, medicinal residues boil 2 times with 8 times of decoctings, each 2h, collecting decoction is concentrated into 0.5:1.Concentrated solution is contained the alcohol amount to 60% with 95% ethanol accent, filter the drying precipitated total polysaccharides position that obtains; After adding 8 times of amount 80% soak with ethanol 0.5h, backflow 2h, 8 layers of filtered through gauze, medicinal residues are with 6 times of 80% alcohol reflux 2 times, at every turn 2h.The rotary evaporation concentrated extracting solution is to 0.5:1, and with 3 defats of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with ethyl acetate equal-volume extraction 3 times, the rotary evaporation extract obtains total flavone part after the drying; With water-saturated n-butanol equal-volume extraction 3 times, the rotary evaporation extract obtains the total saponins position after the drying; Make up by amounting to inventory equivalent in twos at each position simultaneously.
The present invention obtains beyond thought therapeutic effect through animal experiment study, and laboratory report is following:
1 method
1.1 90 of the healthy cleaning level of laboratory animal male mice in kunming, body weight 18-22g purchases laboratory animal company limited animal credit number: the SCXK (capital) 20090819 in Beijing.
1.1 the experimental technique mice is divided into 9 groups (every group each 10) at random: (1) normal control group: normal saline (NS) O.2 mL/10g is irritated stomach, 1 time on the 1st, is total to 7d, subcutaneous injection NS10mg/kg after 7d irritates stomach 2h; (2) ischemia model group: NS irritates the same normal group of stomach, isoproterenol subcutaneous injection 10mg/kg after 7d irritates stomach 2h; (3) anti-CBV Chinese medicine preparation group: anti-CBV Chinese medicine preparation 50mg/kg continuous irrigation stomach 7d.Subcutaneous injection isoproterenol 10mg/kg behind 7d administration 2h, perfusion 1 time again behind the 2h.Each group is irritated gastric capacity and is mL/10g O.2.(4) positive controls: DIAOXINXUE KANG (Chengdu Diao Pharmaceutical Group Co., Ltd; Lot number 0911028) 0.1g/ grain; Face with preceding capsule is shelled; Content is made into DIAOXINXUE KANG solution O.98% with distilled water) solution O .195g/ (kgd), irritating gastric capacity is 0.2 mL/10 g, continuous irrigation stomach 7d.Subcutaneous injection isoproterenol 10mg/kg behind 7d administration 2h, perfusion 1 time again behind the 2h.After giving isoproterenol or normal saline 6h hour, through the femoral artery blood sampling, 4 ℃ of preservations of separation of serum, CPK to be measured, LDH, SOD, MDA activity.
1.3 detection index CK, LDH, SOD, MDA detect by the method that test kit provides.
1.4 all data of statistical analysis are with (
± s) expression of mean ± standard deviation; Each is organized data and uses one factor analysis of variance, examines with t
Test the comparison of organizing a mean.Through the SPSS software processes, < 0.05 expression difference has statistical significance to P.
2 results
About 6h behind the injection isoproterenol; Compare with the normal control group; In the model group mice serum LDH, CK and MDA content obviously raise, SOD content significantly descend (P < 0.05); Compare with model group, LDH, CK and MDA content descend obviously simultaneously that SOD content significantly raises (P < 0.05) in serum of each group of positive controls and anti-CBV Chinese medicine preparation.Polysaccharide+flavone group difference significantly (P < 0.01) wherein.(seeing table 1)
The anti-CBV Chinese medicine preparation of table 1 is to influence (n=10,
± s) of acute myocardial ischemia mice serum zymetology
Embodiment two: dead protection
1 method
1.1 90 of laboratory animal Kunming mouses, body weight 18-22g purchases laboratory animal company limited animal credit number: the SCXK (capital) 20090819 in Beijing.
1.2 1. experimental technique dilutes seed culture of viruses: get the CVB Strain, be diluted to the viral liquid that concentration is about 15 LD50 with physiological saline solution, ice bath is preserved; 2. counteracting toxic substances: mice is divided into 9 groups (every group each 10) at random: be respectively normal group, model group, positive drug group (virazole), anti-CBV Chinese medicine preparation group, except that normal group, all the other only respectively organize about 0.05 mL/ of lumbar injection.Blank control group replaces with the equivalent distilled water; 3. administration: each group all before counteracting toxic substances 1 d begin to irritate the administration of stomach mode.It is that each drug group is irritated stomach amount 0.3 mL/ time, 1 time/d.Blank and model group are then irritated with the equivalent distilled water and are replaced, altogether 12d.4. observe: observe animal morbidity and death toll day by day, observe 15 d altogether.And calculate dead protective rate and prolong vital rates according to observed result.
1.3 statistical analysis is through the SPSS software processes, the time-to-live is used rank test, and survival rate is used χ
2Check.< 0.05 expression difference has statistical significance to P.
2 results
In viral 15 d of the dead protective effect mouse infection of viral induced mice; The dead number average of anti-each treated animal of CBV Chinese medicine preparation is less than the virus group and be not more than positive controls, dead protective rate is organized with virus relatively has significant difference (P 0.05); The average survival natural law of mice virus group obviously prolongs, and increase in life span and virus group relatively have significant difference (P < 0.05), wherein polysaccharide+flavone group difference significantly (P < 0.01).See table 1.
The anti-CBV Chinese medicine preparation of table 1 is to the dead protective effect of contamination mice
Dead protective rate=(model group mortality rate-experimental group mortality rate)/model group mortality rate
Increase in life span=(experimental group on average survive natural law-model group on average survive natural law)/model group natural law of on average surviving
Prove through above embodiment:
1. the present invention can significantly reduce CBV contamination mortality of mice.
2. the present invention has protective effect to CBV contamination rat myocardium from injury.
3. composition effect is better than single position.
4. polysaccharide and flavone composition effect are best.
Claims (2)
1. the compositions of an anti-CBV Chinese medicine preparation effective site is characterized in that comprising total polysaccharides position, total flavone part and total saponins position, and make up by amounting to inventory equivalent in twos at each position; Select Chinese medicine Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae, clean, be ground into coarse powder, can all sieve, but can be no more than 40% through the amount of No. four medicines sieves of 65 purposes through No. two medicines of 20 purposes; Preparation by weight percentage; The percentage by weight of each Chinese medicinal components is following: Radix Panacis Quinquefolii 30-40%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Radix Astragali 30-40%, Cortex Moutan 10-25%, Semen Raphani 5-15%, Semen Vaccariae 4-10%, the percentage by weight sum of above-mentioned each Chinese medicinal components equals 100%; The Chinese medicine coarse powder extracts total polysaccharides position, total flavone part, total saponins position respectively by following three kinds of modes;
1. extract the total polysaccharides position: the Chinese medicine coarse powder adds 6-10 and doubly measures the 90-95% soak with ethanol after 0.5-1 hour, and backflow 1-2 hour, 6-10 layer filtered through gauze, medicinal residues boil 2-4 time with 6-10 times of decocting, and each 2-4 hour, collecting decoction was concentrated into 1-0.5:1; Concentrated solution is contained alcohol with 90-95% ethanol accent measure to 60-80%, filter, the drying precipitated powder that obtains is the total polysaccharides position;
2. extract total flavone part: the Chinese medicine coarse powder adds 6-10 and doubly measures the 70-80% soak with ethanol after 0.5-1 hour, and backflow 2-4 hour, 10 layers of filtered through gauze of 6-, medicinal residues were with 4-8 80% alcohol reflux 2-3 time doubly, 2-4 hour at every turn; The rotary evaporation concentrated extracting solution is to 1-0.5:1, and with 3-6 defat of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with ethyl acetate equal-volume extraction 3-6 time, the rotary evaporation extract obtains powder after the drying, be total flavone part;
3. extract the total saponins position: the Chinese medicine coarse powder adds 6-10 and doubly measures the 70-80% soak with ethanol after 0.5-1 hour, and backflow 2-4 hour, 10 layers of filtered through gauze of 6-, medicinal residues were with 4-8 80% alcohol reflux 2-3 time doubly, 2-4 hour at every turn; The rotary evaporation concentrated extracting solution is to 1-0.5:1, and with 3-6 defat of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with water-saturated n-butanol equal-volume extraction 3-6 time, the rotary evaporation extract obtains powder after the drying, be the total saponins position.
2. the preparation method of composition of an anti-CBV Chinese medicine preparation effective site; It is characterized in that comprising the steps: to select Chinese medicine Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae; Clean; Be ground into coarse powder, can all sieve, but can be no more than 40% through the amount of No. four medicines sieves of 65 purposes through No. two medicines of 20 purposes; Preparation by weight percentage; The percentage by weight of each Chinese medicinal components is following: Radix Panacis Quinquefolii 30-40%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Radix Astragali 30-40%, Cortex Moutan 10-25%, Semen Raphani 5-15%, Semen Vaccariae 4-10%, the percentage by weight sum of above-mentioned each Chinese medicinal components equals 100%; The Chinese medicine coarse powder extracts total polysaccharides position, total flavone part and total saponins position respectively by following three kinds of modes, and make up by amounting to inventory equivalent in twos at each position; Compositions for anti-CBV Chinese medicine preparation effective site;
1. extract the total polysaccharides position: the Chinese medicine coarse powder adds 6-10 and doubly measures the 90-95% soak with ethanol after 0.5-1 hour, and backflow 1-2 hour, 6-10 layer filtered through gauze, medicinal residues boil 2-4 time with 6-10 times of decocting, and each 2-4 hour, collecting decoction was concentrated into 1-0.5:1; Concentrated solution is contained alcohol with 90-95% ethanol accent measure to 60-80%, filter, the drying precipitated powder that obtains is the total polysaccharides position;
2. extract total flavone part: the Chinese medicine coarse powder adds 6-10 and doubly measures the 70-80% soak with ethanol after 0.5-1 hour, and backflow 2-4 hour, 10 layers of filtered through gauze of 6-; Medicinal residues are with 4-8 80% alcohol reflux 2-3 time doubly, and 2-4 hour at every turn, the rotary evaporation concentrated extracting solution was to 1-0.5:1; With 3-6 defat of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with ethyl acetate equal-volume extraction 3-6 time, rotary evaporation extract; Obtain powder after the drying, be total flavone part;
3. extract the total saponins position: the Chinese medicine coarse powder adds 6-10 and doubly measures the 70-80% soak with ethanol after 0.5-1 hour, and backflow 2-4 hour, 10 layers of filtered through gauze of 6-, medicinal residues were with 4-8 80% alcohol reflux 2-3 time doubly, 2-4 hour at every turn; The rotary evaporation concentrated extracting solution is to 1-0.5:1, and with 3-6 defat of boiling range 30-60 ° of C petroleum ether equal-volume extraction, with water-saturated n-butanol equal-volume extraction 3-6 time, the rotary evaporation extract obtains powder after the drying, be the total saponins position.
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| CN1537612A (en) * | 2003-10-24 | 2004-10-20 | 唐山职业技术学院 | Capsule for treating myocarditis of coxsackie B virus type, and its prepn.method |
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| CN1537612A (en) * | 2003-10-24 | 2004-10-20 | 唐山职业技术学院 | Capsule for treating myocarditis of coxsackie B virus type, and its prepn.method |
Non-Patent Citations (2)
| Title |
|---|
| 姚荣妹等.《中药治疗柯萨奇B病毒性心肌炎的研究进展》.《辽宁中医杂志》.2011,第38卷(第1期),187-189. * |
| 邢纪萍的门后.《抗柯萨奇B中药胶囊治疗病毒性心肌炎的临床疗效评价》.《中国临床药理学与治疗学》.2003,第8卷(第6期),701-703. * |
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