CN102008639A - Method for preparing active ingredient of Chinese materia medica preparation for resisting Coxsackie B virus - Google Patents
Method for preparing active ingredient of Chinese materia medica preparation for resisting Coxsackie B virus Download PDFInfo
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Abstract
The invention relates to a method for preparing an active ingredient of a Chinese material medical preparation for resisting Coxsackie B virus, in particular to a method for preparing an active ingredient of a composite Chinese material medical preparation for resisting Coxsackie B virus. The technical scheme is as follows: adding ethanol with the concentration of 60-95% to traditional Chinese medicine coarse powder for soaking for 0.5-1 hour, wherein the addition amount of the ethanol is 6-10 times more than that of the traditional Chinese medicine coarse powder, then generating backflow 2-4 times, lasting for 1-2 hours each time, filtering by 6-10 layers of etamine, merging extract and concentrating the extract to obtain paste, and drying the paste to obtain power, namely the active ingredient of the Chinese material medical preparation for resisting the Coxsackie B virus, wherein and the power comprises the following components in percentage by weight: 20-30% of total astragalan, 30-45% of total saponins, 15-25% of general flavone, 5-10% of lignans, and 5-10% of other components. The method has the beneficial effects of reserving comprehensive functions of each medicinal material ingredient in a compound, being beneficial to exerting pleiotropy of the traditional Chinese medicines, removing tissue matters and parts of inactive ingredients, prolonging storage life, improving purity, reducing dosage, improving reproducibility of quality control, and being beneficial to industrialized and standardized production. The method has the advantages of clear curative effects, high efficiency, low toxicity, obvious effect for resisting the Coxsackie B virus and the like, and is convenient to take.
Description
Technical field
The present invention relates to a kind of effective ingredient preparation method of Chinese traditional compound medicine, the effective ingredient preparation method of particularly anti-Coxsackie B virus Chinese traditional compound medicine.
Background technology
Coxsackie virus is a kind of in the more common virus of nature, can often cause diseases such as respiratory tract infection, intestinal infection, aseptic meningitis, myocarditis, pericarditis through respiratory tract and digestive tract infection human body.Simultaneously, in utero Coxsackie virus infection also is one of high risk factor that brings out congenital heart disease.Children's and between twenty and fifty susceptible, especially infant during this century, Coxsackie virus was very popular for several times both at home and abroad, and involve heart more.Doctor trained in Western medicine does not have specific treatment to viral myocarditis, and the slow intravenous injection of general heavy dose of high concentration vitamin C promotes cardiomyopathy to recover.Commonly used for many years promotes that as polarized solution, Energy mixture and ATP etc. the energy of ischemic myocardial cells is synthetic, stablize the damaged myocardium cell membrane, improves myocardium pumping function, reduces the lipid peroxide generation, improves myocardial ischemia, alleviates the tissue injury due to the cardiac muscle.But, can not give full play to the effect of protection cardiac muscle all because of myocardial cell difficult to get access.Although multiple single medicinal material has the effect of anti-Coxsackie B virus, now for clinical use be the Chinese medicine crude drug, do not have the fixed prescription preparation, cause taking dose bigger, the medical material waste.Chinese invention patent: ZL
200310101631.4,Title " anti-Coxsackie B virus myocarditis capsule and preparation method thereof " has solved the problems referred to above, and its Chinese medicine weight percentages of components is as follows: Radix Panacis Quinquefolii 15-30%, Semen Raphani 5-15%, Semen Vaccariae 4-10%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Cortex Moutan 10-25%, the Radix Astragali 30-40%.But there are the following problems for background technology: the Chinese medicine taking dose is big, stores inconvenience, and the storage time is short.
Summary of the invention
The object of the invention provides a kind of effective ingredient preparation method of anti-Coxsackie B virus Chinese medicine preparation, both kept the comprehensive function of each medicinal ingredient in the compound recipe, help bringing into play the pleiotropy of Chinese medicine, suck tissue and partial invalidity composition have been removed again, prolong the storage life, improved purity, reduced dose, the repeatability of quality control improves, and solves the problems referred to above that background technology exists.
The present invention seeks to realize by following technical scheme:
A kind of effective ingredient preparation method of anti-Coxsackie B virus Chinese medicine preparation comprises the steps:
1. material choice is selected Chinese medicine Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae, cleans, and is ground into the Chinese medicine coarse powder, can all sieve by No. two medicines of 20 purposes, but can be no more than 40% by the amount of No. four medicines sieves of 65 purposes; 2. prepare the Chinese medicine coarse powder by weight percentage, the percentage by weight of each Chinese medicinal components is as follows: Radix Panacis Quinquefolii 30-40%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Radix Astragali 30-40%, Cortex Moutan 10-25%, Semen Raphani 5-15%, Semen Vaccariae 4-10%; 3. after the Chinese medicine coarse powder adds the 60-95% soak with ethanol 0.5-1h that 6-10 doubly measures, backflow 2-4 time, 1-2h at every turn, 6-10 layer filtered through gauze, merge extractive liquid, is condensed into cream, and drying obtains powder, is the effective ingredient of anti-Coxsackie B virus Chinese medicine preparation; 4. each weight percentages of components is in the above-mentioned powder: total polysaccharides 20-30%, total saponins 30-45%, total flavones 15-25%, total lignans 5-10%, other 5-10%.
Preferred version of the present invention: the Chinese medicine coarse powder refluxes 3 times after adding the 75-85% soak with ethanol 0.5h of 8 times of amounts, each 1.5h, centrifugal, 8 layers of filtered through gauze, merge extractive liquid, is condensed into cream, drying obtains powder, is the effective ingredient of anti-Coxsackie B virus Chinese medicine preparation.
The gained powder is prepared into clinical required dosage form with the adjuvant that suits and uses.
Said No. two medicines sieve, No. four medicine sieves are the medicinal sieve of standard, and said times of amount is volume ratio.
The present invention can be prepared into the various dosage forms of anti-Coxsackie B virus with Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae compatibility, and the medicine utilization rate of active components is obviously improved.
Radix Panacis Quinquefolii is cold in nature, sweet and slightly bitter taste, people's lung, spleen channel.Have boosting qi and nourishing yin, the relieving restlessness of relieving inflammation or internal heat, support effect for promoting the production of body fluid.
Fructus Schisandrae Chinensis sour in the mouth, sweet, warm in nature, return lung, the heart, kidney channel.It is astringent or styptic treatment for spontaneous sweating to have convergence, supplementing QI for promoting the production of body fluid, and the effect of kidney calming,
Radix Ophiopogonis sweet and slightly bitter taste, cold nature, GUIXIN, lung, stomach warp.Has YIN nourishing and the production of body fluid promoting, the effect that lung moistening clears away heart-fire.
Radix Astragali sweet in the mouth, slightly warm in nature is returned lung, spleen, liver, kidney channel.There are benefiting QI for strengthening the superficies, arresting sweating to take off, hold in the palm the effect of skin ulcer granulation promoting, inducing diuresis to remove edema admittedly.
Cortex Moutan bitter in the mouth, suffering, cold nature, GUIXIN, liver, kidney channel.Has clearing away heat and cooling blood; The effect of promoting blood circulation to remove blood stasis.
Semen Raphani acrid in the mouth, sweet, property is flat.Return lung, spleen, stomach warp.Having and helping digestion except that expanding the effect of lowering the adverse-rising QI to resolve phlegm.
The Semen Vaccariae bitter in the mouth, property is flat.Return liver, the stomach warp.Promoting blood circulation to restore menstrual flow is arranged, stimulating milk secretion eliminating carbuncle, the effect of inducing diuresis for treating stranguria syndrome.
Can reduce the sensitivity of cardiac muscle to virus entirely, human body immunity improving power and anti-virus ability have heart tonifying, reduce effects such as oxygen consumes, correction arrhythmia.
The invention has the beneficial effects as follows: both kept the comprehensive function of each medicinal ingredient in the compound recipe, help bringing into play the pleiotropy of Chinese medicine, suck tissue and partial invalidity composition have been removed again, prolong the storage life, improved purity, reduced dose, the repeatability of quality control improves, and helps industrialization, standardized production; The present invention has characteristics such as curative effect is clear and definite, high-efficiency low-toxicity, taking convenience, and anti-Coxsackie B virus effect is remarkable.
The specific embodiment
The invention will be further described by the following examples.
In an embodiment, a kind of effective ingredient preparation method of anti-Coxsackie B virus Chinese medicine preparation, comprise as follows: 1. material choice, select Chinese medicine Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae, clean, be ground into the Chinese medicine coarse powder, can all sieve, but can be no more than 40% by the amount of No. four medicines sieves of 65 purposes by No. two medicines of 20 purposes; 2. prepare the Chinese medicine coarse powder by weight percentage, the percentage by weight of each Chinese medicinal components is as follows: Radix Panacis Quinquefolii 30-40%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Radix Astragali 30-40%, Cortex Moutan 10-25%, Semen Raphani 5-15%, Semen Vaccariae 4-10%; 3. after the Chinese medicine coarse powder adds the 60-95% soak with ethanol 0.5-1h that 6-10 doubly measures, backflow 2-4 time, 1-2h at every turn, 6-10 layer filtered through gauze, merge extractive liquid, is condensed into cream, and drying obtains powder, is the effective ingredient of anti-Coxsackie B virus Chinese medicine preparation; 4. each weight percentages of components is in the above-mentioned powder: total polysaccharides 20-30%, total soap: 30-45%, total flavones 15-25%, total lignans 5-10%, other 5-10%.
The present invention obtains beyond thought therapeutic effect by animal experiment study, and laboratory report is as follows:
One, myocardial preservation
1, method
1.1 laboratory animal: 60 of healthy cleaning level male SD rats, body weight 240-260g purchases in Beijing laboratory animal company limited animal credit number: the SCXK(capital) 20090330.
1.2 experimental technique: rat is divided into 4 groups (every group each 15) at random: (1) normal control group: normal saline (NS) 30ml/kg irritates stomach, 1 time on the 1st, is total to 7d, subcutaneous injection NS30ml/kg after 7d irritates stomach 2h; (2) ischemia model group: NS irritates the same normal group of stomach, isoproterenol subcutaneous injection 30ml/kg after 7d irritates stomach 2h; (3) anti-Coxsackie B virus Chinese medicine preparation group: anti-Coxsackie B virus Chinese medicine preparation 20mg/ ml is dissolved in the normal saline,, with 4ml/kg continuous irrigation stomach 7d.Subcutaneous injection isoproterenol 30ml/kg behind 7d administration 2h, perfusion 1 time again behind the 2h.(4) positive controls: nifedipine 5mg/ ml is dissolved in the normal saline,, with 4ml/kg continuous irrigation stomach 7d.Subcutaneous injection isoproterenol 30ml/kg behind 7d administration 2h, perfusion 1 time again behind the 2h.Each group is all traced normal ECG before administration, give isoproterenol or normal saline 6h hour after, the replication electrocardiogram, then through femoral artery blood sampling, separation of serum, 4 ℃ of preservations, CPK to be measured, LDH, SOD, MDA activity.
1.3 detection index
1.3.1 detecting ECG: each treated animal writes down normal II (the chart speed 50mm/s that leads, calibration 1mV=10mm), after measuring the subcutaneous injection isoproterenol, unusual total millivolt number (∑ ST), the ST field offset meansigma methods of being offset of ST section is as the index of judging degree of myocardial ischemia and evaluation curative effect of medication.
1.3.2 the mensuration of CPK, LDH, SOD, MDA: the method that provides by test kit detects.
1.4 statistical analysis: all data with mean ± standard deviation (
± s) expression, each is organized data and uses one factor analysis of variance, organizes the comparison of a mean with the t check.Through the SPSS software processes, P＜0.05 expression difference has statistical significance.
2, result
2.1 influence and normal control group to rat ECG ST field offset compare model control group ST section significance skew (P＜0.01); With model control group relatively, anti-Coxsackie B virus Chinese medicine preparation group, Nifedipine group all can significantly reduce the unusual skew (P＜0.05) of the ST section due to the ISO.See Table 1.
The anti-Coxsackie B virus Chinese medicine preparation of table 1 to acute myocardial ischemia rat II lead the ST field offset influence (n=15,
± s)
Annotate: compare with matched group
▲P＜0.01; Compare with model group
△P＜0.05
2.2 to about 6h behind the influence injection isoproterenol of acute myocardial ischemia rat blood serum zymetology, compare with the normal control group, in the model group rat blood serum LDH, CK and MDA content obviously raise, SOD content significantly descend (P＜0.05), compare LDH, CK and the MDA content SOD content significantly raise (P＜0.05) that obviously descends simultaneously in the serum of anti-Coxsackie B virus Chinese medicine preparation group with model group.Positive controls also has the trend that reduces LDH, CK, MDA activity and SOD activity improving, but compares not statistically significant with model control group.(seeing Table 2)
The anti-Coxsackie B virus Chinese medicine preparation of table 2 to the influence of acute myocardial ischemia rat blood serum zymetology (n=15,
± s)
Annotate: compare with matched group
▲P＜0.05; Compare with model group
△P＜0.05
Two, dead protection
1, method
1.1 laboratory animal: 40 of Kunming mouses, body weight 18-22g purchases in Beijing laboratory animal company limited animal credit number: the SCXK(capital) 20090609.
1.2 experimental technique: 1. dilute seed culture of viruses: get the CVB Strain, be diluted to the viral liquid that concentration is about 15 LD50 with physiological saline solution, ice bath is preserved; 2. counteracting toxic substances: mice is divided into 4 groups (every group each 10) at random: be respectively normal group, infection group and viral infection group, positive drug group (virazole), anti-Coxsackie B virus Chinese medicine preparation group, except that normal group, all the other only respectively organize about 0.05 mL/ of lumbar injection.Normal group replaces with the equivalent distilled water; 3. administration: each group all before counteracting toxic substances 1 d begin to irritate stomach mode administration (drug level 1.0g/ mL).It is that each medicine group is irritated stomach amount 0.3 mL/ time, 1 time/d.Normal and infection group and viral infection group is then irritated with the equivalent distilled water and is replaced, altogether 12d.4. observe: observe animal morbidity and death toll day by day, observe 15 d altogether.And the result calculates dead protective rate and prolongs vital rates according to the observation.
1.3 statistical analysis: through the SPSS software processes, the time-to-live is used rank test, survival rate χ
2Check.P＜0.05 expression difference has statistical significance.
2, result
Protective effect to viral induced mice death: in mouse infection virus 15 d, the dead number average of anti-Coxsackie B virus Chinese medicine preparation treated animal is less than infection group and viral infection group, dead protective rate is respectively 55.56%, with infection group and viral infection group significant difference (P＜0.05) is arranged relatively; The average survival natural law of mice obviously prolongs than infection group and viral infection group, with infection group and viral infection group significant difference (P＜0.05) is arranged relatively, and increase in life span is 80%.See Table 1.
The anti-Coxsackie B virus Chinese medicine preparation of table 1 is to the dead protective effect of contamination mice
Dead protective rate=(infection group and viral infection group mortality rate-experimental group mortality rate)/infection group and viral infection group mortality rate.
Increase in life span=(experimental group on average survive natural law-infection group and viral infection group on average survive natural law)/infection group and viral infection group natural law of on average surviving.
Prove by above embodiment:
1. the present invention can significantly reduce Coxsackie B virus contamination mortality of mice;
2. the present invention has protective effect to rat myocardium from injury.
Claims (3)
1. the effective ingredient preparation method of an anti-Coxsackie B virus Chinese medicine preparation, it is characterized in that comprising as follows: 1. material choice, select Chinese medicine Radix Panacis Quinquefolii, Fructus Schisandrae Chinensis, Radix Ophiopogonis, the Radix Astragali, Cortex Moutan, Semen Raphani, Semen Vaccariae, clean, be ground into the Chinese medicine coarse powder, can all sieve, but can be no more than 40% by the amount of No. four medicines sieves of 65 purposes by No. two medicines of 20 purposes; 2. prepare the Chinese medicine coarse powder by weight percentage, the percentage by weight of each Chinese medicinal components is as follows: Radix Panacis Quinquefolii 30-40%, Fructus Schisandrae Chinensis 4-15%, Radix Ophiopogonis 5-20%, Radix Astragali 30-40%, Cortex Moutan 10-25%, Semen Raphani 5-15%, Semen Vaccariae 4-10%; 3. after the Chinese medicine coarse powder adds the 60-95% soak with ethanol 0.5-1h that 6-10 doubly measures, backflow 2-4 time, 1-2h at every turn, 6-10 layer filtered through gauze, merge extractive liquid, is condensed into cream, and drying obtains powder, is the effective ingredient of anti-Coxsackie B virus Chinese medicine preparation; 4. each weight percentages of components is in the above-mentioned powder: total polysaccharides 20-30%, total saponins 30-45%, total flavones 15-25%, total lignans 5-10%, other 5-10%.
2. according to the effective ingredient preparation method of the described anti-Coxsackie B virus Chinese medicine preparation of claim 1, after it is characterized in that the Chinese medicine coarse powder adds the 75-85% soak with ethanol 0.5h of 8 times of amounts, reflux 3 times, each 1.5h, centrifugal, 8 layers of filtered through gauze, merge extractive liquid, is condensed into cream, drying obtains powder, is the effective ingredient of anti-Coxsackie B virus Chinese medicine preparation.
3. according to the effective ingredient preparation method of claim 1 or 2 described anti-Coxsackie B virus Chinese medicine preparation, it is characterized in that gained powder and suitable adjuvant are prepared into clinical required dosage form and use.
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|---|---|---|---|---|
| CN1537612A (en) * | 2003-10-24 | 2004-10-20 | 唐山职业技术学院 | Capsule for treating myocarditis of coxsackie B virus type, and its prepn.method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537612A (en) * | 2003-10-24 | 2004-10-20 | 唐山职业技术学院 | Capsule for treating myocarditis of coxsackie B virus type, and its prepn.method |
Non-Patent Citations (1)
| Title |
|---|
| 《中国全医科学》 20100831 余红 等 病毒性心肌炎临床治疗进展 2770-2772 1-3 第13卷, 第8C期 2 * |
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Application publication date: 20110413 |