CN104397359B - A kind of chelating amino acids calcium composition and preparation method thereof - Google Patents
A kind of chelating amino acids calcium composition and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of chelating amino acids calcium composition and preparation method thereof, said composition includes 32~48 parts of compound amino acid chelate calcium, 0.0001~0.001 part of vitamin D, 0.001~0.01 part of vitamin K, 24~36 parts of glycine, 24~36 parts of proline composition, 5~15 parts of probiotics, 10~20 parts of plant extracts etc., it promotes calcium uptake using vitamin, with amino acid, polysaccharide promotes bone matrix to be formed, addition probiotics provides good intestinal environment for the absorption of calcium, addition plant extracts not only improves the calcium uptake of body, it is again to have anti-oxidant more, detoxicating, relieving inflammation, improve the effect of immunity of organisms, therefore act synergistically to reach purpose of replenishing the calcium from many aspects, more conducively calcium uptake, it is non-stimulated to enteron aisle, avoid the problems such as digestive discomfort or constipation, and preparation method is simple, the former nutritional ingredient of each component can be retained, each nutritional ingredient is set to act synergistically, absorptivity is high.
Description
Technical field
The present invention relates to a kind of chelating amino acids calcium composition and preparation method thereof.
Background technology
At present, calcium is that people's in-vivo content is most and most active metallic element, after occupying oxygen, carbon, hydrogen, nitrogen in vivo, row
At the 5th.The 2% of body weight is accounted for, total amount has the good reputation of human body " biological element " more than 1 kilogram.Calcium is most rich in human body
Rich mineral matter, participates in the whole life process of human body, from the think of of bone formation, contraction of muscle, heartbeat, nerve and brain
Dimension activity, until the growing of human body, dispelling fatigue, brain tonic and intelligence development and anti-aging etc., it may be said that all fortune of life
It is dynamic all to be unable to do without calcium.Thus " calcium is human life sheet ".
Calcium deficiency is to the resistance of human body, endurance, immunity, memory, comprehension, creativity, respond and anti-aging
Ability has different degrees of influence.Furtherly, osteoproliferation, osteoporosis, fracture, rickets, dental caries, scapulohumeral periarthritis,
The diseases such as arthritis, retarded juvenile, senile dementia, hypertension, heart disease, diabetes, anemia, apositia all with calcium deficiency
There is direct or indirect relation.
The development history of calcium supplementing product is made a general survey of, calcium supplementing product substantially experienced following four developing stage：(1) inorganic calcium rank
Section, the inorganic calcium salts such as calcium carbonate, calcium monohydrogen phosphate are supplemented to human body.(2) the organic calcium stage, calcium lactate, glucose are supplemented to human body
The organic calciums such as sour calcium, l threonic acid, to increase the intake of calcium.(3) calcium+vitamin D stage, strengthen simultaneously in calcium supplementing product
Vitamin D, to improve the absorption rate of calcium.(4) calcium+CPP stages, substituted with CPP (CPP) has one to human body
Determine the VD of toxic side effect (when being particularly Excess free enthalpy).
All it is the single varieties to replenish the calcium in this numerous calcium supplementing product, is all calcium plus vitamin or trace element
Deng product, mechanism of action is single, the collaboration facilitation between no number of mechanisms, causes the calcium agent that many reasons are caused to be difficult to inhale
Receive, do not reach curative effect.
The content of the invention
Intend in view of the above problems, the present invention provides a kind of from vitamin level, bone matrix and important component, calcium uptake intestines
The many-side such as road environment acts synergistically to reach the chelating amino acids calcium composition of many mechanism of purpose of replenishing the calcium.
Present invention also offers a kind of preparation method of above-mentioned chelating amino acids calcium composition.
To achieve these goals, the technical solution adopted in the present invention be the chelating amino acids calcium composition include with
The raw material of lower proportion by weight：
Above-mentioned proline composition is to be combined by hydroxyproline with proline, and hydroxyproline：The quality of proline
Than for 1：1~3；Show through Amino acid sequence analysis, each bar peptide chain of collagen all contains Gly-X-Por or Gly-x-HyP
Tripeptides repetitive sequence, wherein, X can be any amino acid, and HyP is the hydroxylation derivant of proline.Present invention addition
Glycine, proline and hydroxyproline, make the ratio of glycine, proline and hydroxyproline, other amino acid control 1:
1:1 or so, the ratio of each amino acid close in human collagen protein sequence, the hydroxylation of proline residue is to three strands of spiral shells
The robustness of rotation plays an important roll, and the chain that hydroxylation is not enough can not form firm triple helix under body temperature, therefore can not be from
Discharged in cell, provide good environment and material base for body formation collagen, bone collagen can be promoted
Formation.
Above-mentioned probiotics can be Bifidobacterium or Bacillus acidi lactici or its any combination；
Above-mentioned plant extracts can be the sophora bud, chrysanthemum, dried orange peel, hawthorn, tomato, grape, screw oil expeller axle, soybean, the Radix Astragali, Pueraria lobota
Root, ginkgo leaf, barrenwort, one kind or any combination of belamcanda chinensis extract.
The mucopolysaccharide or astragalus polyose of 10~20 parts by weight can also be included in combinations of the above thing；Mucopolysaccharide is sulfuric acid
Chondroitin or hyaluronic acid, contribute to bone matrix to be formed,
The raw material composition of said composition preferably following proportion by weight：
Present invention also offers a kind of preparation method of above-mentioned chelating amino acids calcium composition, it is comprised the steps of：
(1) coarse crushing after fresh ox bone is cleaned, carries out degreasing to ox bone, boiling seasonings ox bone is with steaming using autoclaving method
The volume ratio of distilled water is 1:4~6, preferably 1:5, temperature is 121 DEG C, and the time is 1.5~2h；
(2) the fresh ox bone after boiling is drained into profit, evenly laid out on square position, in the baking oven for being put into 80 DEG C, 10h dries
It is dry, through multi-stage crushing, cross 80 mesh sieves and obtain bovine bone powder, be in mass ratio 1:8~12 amount adds 2mol/L's into bovine bone powder
Hydrochloric acid, preferably 1:1~2h of acidolysis, suction filtration at 10,70 DEG C, obtain the supernatant containing solubility calcium, add NaOH regulation supernatants pH
To 8.0, by compound amino acid and Ca2+Mass ratio be 2.5:1~3.5:1 amount adds compound amino acid, preferably 3:1,75~
Reacted at 85 DEG C and 70min is reacted at 60~80min, preferably 80 DEG C, compound amino acid chelate calcium is obtained after drying；
(3) weigh according to the above ratio containing compound amino acid chelate calcium, vitamin D, vitamin K, glycine, proline and hydroxyl
Proline, probiotics, plant extracts and polysaccharide, are sufficiently mixed, and chelating amino acids calcium composition is made.
The chelating amino acids calcium composition of the present invention is a kind of calcium agent composition of many mechanism, and it promotes calcium using vitamin
Absorb, promote bone matrix to be formed with amino acid, polysaccharide, wherein amino acid especially glycine, proline and hydroxyproline is bone
The important composition composition of collagen, the present invention exists the ratio control of glycine, proline and hydroxyproline, other amino acid
1:1:1 or so, each amino acid ratio close in human collagen protein sequence is provided well for body formation collagen
Environment and material base, are the main components of bone matrix and phoirocyte using polysaccharide especially mucopolysaccharide, beneficial to bone base
Matter is formed, while the present invention also added probiotics, good intestinal environment, the sophora bud, hawthorn, chrysanthemum etc. are provided for the absorption of calcium
Plant extracts not only improves the calcium uptake of body, and plays the role of anti-oxidant, detoxicating, relieving inflammation more, improves immunity of organisms, enters
One step safeguards the unique effect of body health, therefore acts synergistically from many aspects to reach purpose of replenishing the calcium, more conducively calcium uptake, and
And it is non-stimulated to enteron aisle, the problems such as digestive discomfort or constipation will not be produced；The preparation method of the present invention is simple, can retain each
The former nutritional ingredient of component, makes each nutritional ingredient act synergistically, and absorptivity is high.
Embodiment
Technical scheme is further described in conjunction with embodiment and data, but the present invention is not limited only to
Following implementation situations.
Embodiment 1
Now so that every part is 1g as an example, the chelating amino acids calcium composition of the present embodiment is made up of the raw material of following proportion by weight：
One kind of the one kind, as aminoglucan of the chondroitin sulfate of the present embodiment actually in mucopolysaccharide, is bone matrix
With the main component of phoirocyte, the dissacharide units that it is made up of uronic acid and N-acetylhexosamine or its sulfuric ester
Repetitive sequence macromolecular, be the glycosaminoglycan of polyanionic polymer.
It by hydroxyproline and proline according to mass ratio is 1 that the proline composition of the present embodiment, which is,:2 amount composition, i.e.,
Hydroxyproline is 10g, and proline is 20g.
Above-mentioned Huai Goat is according to the extract obtained by conventional extraction process extraction by the sophora bud.
Above-mentioned chelating amino acids calcium composition is comprised the steps of：
(1) coarse crushing after fresh ox bone is cleaned, carries out degreasing to ox bone, boiling seasonings ox bone is with steaming using autoclaving method
The volume ratio of distilled water is 1:5, temperature is 121 DEG C, and the time is 1.5h；
(2) the fresh ox bone after boiling is drained into profit, evenly laid out on square position, in the baking oven for being put into 80 DEG C, 10h dries
It is dry, through multi-stage crushing, cross 80 mesh sieves and obtain bovine bone powder, be in mass ratio 1:10 amount adds concentration into bovine bone powder for 2mol/L
Hydrochloric acid, acidolysis 1.5h at 70 DEG C, suction filtration obtains the supernatant containing solubility calcium, adds NaOH and adjust supernatant pH to 8.0, press
Compound amino acid and Ca2+Mass ratio be 3:1 amount, which is added, reacts 70min at compound amino acid, 80 DEG C, ammonia must be combined after drying
Base acid chelating calcium；
(3) weigh compound amino acid chelate calcium 40g according to the above ratio, it is vitamin D 0.0005g, vitamin K 0.005g, sweet
Propylhomoserin 30g, hydroxyproline 10g, proline 20g, Bifidobacterium 10g, Huai Goat 15g and chondroitin sulfate 15g, fully
Mixing, is made chelating amino acids calcium composition.
Embodiment 2
Now so that every part is 1g as an example, the chelating amino acids calcium composition of the present embodiment is made up of the raw material of following proportion by weight：
It by hydroxyproline and proline according to mass ratio is 1 that the proline composition of the present embodiment, which is,:1 amount composition, i.e.,
Hydroxyproline is 14g, and proline is 14g.
Above-mentioned chelating amino acids calcium composition is comprised the steps of：
(1) coarse crushing after fresh ox bone is cleaned, carries out degreasing to ox bone, boiling seasonings ox bone is with steaming using autoclaving method
The volume ratio of distilled water is controlled 1:4, temperature is 121 DEG C, and the time is 2h；
(2) the fresh ox bone after boiling is drained into profit, evenly laid out on square position, in the baking oven for being put into 80 DEG C, 10h dries
It is dry, through multi-stage crushing, cross 80 mesh sieves and obtain bovine bone powder, be in mass ratio 1:8 amount adds concentration into bovine bone powder for 2mol/L
Hydrochloric acid, acidolysis 1h at 70 DEG C, suction filtration obtains the supernatant containing solubility calcium, NaOH regulation supernatant pH to 8.0 added, by multiple
Close amino acid and Ca2+Mass ratio be 2.5:1 amount, which is added, reacts 80min at compound amino acid, 75 DEG C, ammonia must be combined after drying
Base acid chelating calcium；
(3) weigh compound amino acid chelate calcium 38g according to the above ratio, it is vitamin D 0.0003g, vitamin K 0.003g, sweet
Propylhomoserin 28g, hydroxyproline 14g, proline 14g, Bifidobacterium 8g, Huai Goat 18g and chondroitin sulfate 18g, fully
Mixing, is made chelating amino acids calcium composition.
Embodiment 3
Now so that every part is 1g as an example, the chelating amino acids calcium composition of the present embodiment is made up of the raw material of following proportion by weight：
It by hydroxyproline and proline according to mass ratio is 1 that the proline composition of the present embodiment, which is,:3 amount composition, i.e.,
Hydroxyproline is 8.25g, and proline is 24.75g.
Astragalus polyose used in the present embodiment be using legume astragalus mongolicus or Astragalus membranacus dried roots according to
The water-soluble heteroglycan that common process is extracted, concentrates, obtained after purification, wherein containing various active monosaccharide components such as D- glucose
Sugar, D- galactolipins, L-arabinose etc., and these are present in conjunction of the monose in human body in cartilage cell epimatrix proteoglycan
Important substrate is act as in.
Above-mentioned chelating amino acids calcium composition is comprised the steps of：
(1) coarse crushing after fresh ox bone is cleaned, carries out degreasing to ox bone, boiling seasonings ox bone is with steaming using autoclaving method
The volume ratio of distilled water is controlled 1:6, temperature is 121 DEG C, and the time is 1.8h；
(2) the fresh ox bone after boiling is drained into profit, evenly laid out on square position, in the baking oven for being put into 80 DEG C, 10h dries
It is dry, through multi-stage crushing, cross 80 mesh sieves and obtain bovine bone powder, be in mass ratio 1:12 amount adds concentration into bovine bone powder for 2mol/L
Hydrochloric acid, acidolysis 2h at 70 DEG C, suction filtration obtains the supernatant containing solubility calcium, NaOH regulation supernatant pH to 8.0 added, by multiple
Close amino acid and Ca2+Mass ratio be 3.5:1 amount, which is added, reacts 60min at compound amino acid, 85 DEG C, ammonia must be combined after drying
Base acid chelating calcium；
(3) weigh compound amino acid chelate calcium 43g according to the above ratio, it is vitamin D 0.0006g, vitamin K 0.006g, sweet
Propylhomoserin 33g, hydroxyproline 8.25g, proline 24.75g, Bacillus acidi lactici 13g, Astragalus Root P.E 16g and astragalus polyose 16g,
It is sufficiently mixed, chelating amino acids calcium composition is made.
Embodiment 4
Now so that every part is 1g as an example, the chelating amino acids calcium composition of the present embodiment by following proportion by weight raw material group
Into：
It by hydroxyproline and proline according to mass ratio is 1 that the proline composition of the present embodiment, which is,:3 amount composition, i.e.,
Hydroxyproline is 6g, and proline is 18g.
Probiotics used in the present embodiment is that Bifidobacterium and Bacillus acidi lactici are 1 according to mass ratio:1 amount is combined.
Above-mentioned chelating amino acids calcium composition is comprised the steps of：
(1) it is identical with above-described embodiment 1.
(2) it is identical with above-described embodiment 1, obtain compound amino acid chelate calcium；
(3) weigh compound amino acid chelate calcium 32g according to the above ratio, it is vitamin D 0.0001g, vitamin K 0.001g, sweet
Propylhomoserin 24g, hydroxyproline 6g, proline 18g, Bacillus acidi lactici 2.5g, Bifidobacterium 2.5g, kudzu root extract 10g and transparent
Matter acid 10g, is sufficiently mixed, and chelating amino acids calcium composition is made.
Embodiment 5
Now so that every part is 1g as an example, the chelating amino acids calcium composition of the present embodiment by following proportion by weight raw material group
Into：
It by hydroxyproline and proline according to mass ratio is 1 that the proline composition of the present embodiment, which is,:2 amount composition, i.e.,
Hydroxyproline is 12g, and proline is 24g.
Probiotics used in the present embodiment is that Bifidobacterium and Bacillus acidi lactici are 1 according to mass ratio:4 amount is combined.
Above-mentioned chelating amino acids calcium composition is comprised the steps of：
(1) it is identical with above-described embodiment 1.
(2) it is identical with above-described embodiment 1, obtain compound amino acid chelate calcium；
(3) compound amino acid chelate calcium 48g, vitamin D 0.001g, vitamin K 0.01g, sweet ammonia are weighed according to the above ratio
Sour 36g, hydroxyproline 12g, proline 24g, Bacillus acidi lactici 3g, Bifidobacterium 12g, grape extract 20g and hyaluronic acid
20g, is sufficiently mixed, and chelating amino acids calcium composition is made.
Embodiment 6
Now so that every part is 1g amount as an example, the chelating amino acids calcium composition of the present embodiment by following proportion by weight original
Material composition：
It by hydroxyproline and proline according to mass ratio is 1 that the proline composition of the present embodiment, which is,:1 amount composition, i.e.,
Hydroxyproline is 16g, and proline is 16g.
Above-mentioned chelating amino acids calcium composition is comprised the steps of：
(1) it is identical with above-described embodiment 1.
(2) it is identical with above-described embodiment 1, obtain compound amino acid chelate calcium；
(3) weigh compound amino acid chelate calcium 39g according to the above ratio, it is vitamin D 0.0004g, vitamin K 0.004g, sweet
Propylhomoserin 32g, hydroxyproline 16g, proline 16g, Bacillus acidi lactici 12g and extract of soybean 15g, are sufficiently mixed, and amino is made
Acid chelating calcium composition.
Embodiment 7
Now so that every part is 1g amount as an example, the chelating amino acids calcium composition of the present embodiment by following proportion by weight original
Material composition：
It by hydroxyproline and proline according to mass ratio is 1 that the proline composition of the present embodiment, which is,:1 amount composition, i.e.,
Hydroxyproline is 15g, and proline is 15g.
Above-mentioned chelating amino acids calcium composition is comprised the steps of：
(1) it is identical with above-described embodiment 1.
(2) it is identical with above-described embodiment 1, obtain compound amino acid chelate calcium；
(3) weigh compound amino acid chelate calcium 42g according to the above ratio, it is vitamin D 0.0003g, vitamin K 0.003g, sweet
Propylhomoserin 29g, hydroxyproline 15g, proline 15g, Bifidobacterium 9g, belamcanda chinensis extract 10g and Shorthorned Epimedium P.E 3g, fully
Mixing, is made chelating amino acids calcium composition.
Embodiment 8
Now so that every part is 1g amount as an example, the chelating amino acids calcium composition of the present embodiment by following proportion by weight original
Material composition：
It by hydroxyproline and proline according to mass ratio is 1 that the proline composition of the present embodiment, which is,:1.5 amount composition,
I.e. hydroxyproline is 12g, and proline is 18g.
Above-mentioned chelating amino acids calcium composition is comprised the steps of：
(1) it is identical with above-described embodiment 1.
(2) it is identical with above-described embodiment 1, obtain compound amino acid chelate calcium；
(3) weigh compound amino acid chelate calcium 38g according to the above ratio, it is vitamin D 0.0005g, vitamin K 0.005g, sweet
Propylhomoserin 31g, hydroxyproline 12g, proline 18g, Bacillus acidi lactici 12g, chrysanthemum extract 8g and Huai Goat 6g, it is fully mixed
Close, chelating amino acids calcium composition is made.
Plant extracts in above-described embodiment can also with etc. quality dried orange peel, hawthorn, tomato, screw oil expeller axle, ginkgo leaf
Or the extract of barrenwort is replaced, or can also with etc. quality the sophora bud, chrysanthemum, dried orange peel, hawthorn, tomato, grape, screw oil expeller
Axle, soybean, the Radix Astragali, the root of kudzu vine, ginkgo leaf, barrenwort, the arbitrary proportion composition of belamcanda chinensis extract are replaced, due to the sophora bud, chrysanthemum
Flower, dried orange peel, hawthorn, tomato, grape, screw oil expeller axle, soybean, the Radix Astragali, the root of kudzu vine, ginkgo leaf, barrenwort, blackberry lily etc. are different containing what is enriched
The absorption of flavones, more conducively calcium, by increasing capacitance it is possible to increase bone density, in addition, also anti-oxidant, detoxicating, relieving inflammation, the effect of raising immunity,
Therefore, each Plant Extracts effect that it rises in chelating amino acids calcium composition of the invention is identical.
The modes such as chelating amino acids calcium composition of the present invention can pelletize, film-making or glue capsule are individually oral, or can be with
Added when rushing milk powder or preparing food edible.
In order to verify that the chelating amino acids calcium composition of the present invention has preferable effect, inventor passes through substantial amounts of experiment
Verified, for details, reference can be made to following part Experiment and result is illustrated.
1st, conventional detection is tested
According to detection method as defined in GB 14881 and solid beverage examination of production licence detailed rules and regulations standard to of the invention real
Apply the composition prepared by example 1,3,6,8 to be detected, as a result such as table 1 below：
Table 1 is the conventional detection result of the chelating amino acids calcium composition of the present invention
| Project | Index | Embodiment 1 | Embodiment 3 | Embodiment 6 | Embodiment 8 |
| Calcium, % | 2~5 | 2.7 | 2.6 | 2.8 | 2.3 |
| Amino acid, % >= | 40 | 67.1 | 62.3 | 71.5 | 71.2 |
| Vitamin D, μ g/kg >= | 1500 | 2000 | 1800 | 2000 | 2000 |
| Vitamin E, mg α-TE/kg >= | 21 | 22 | 22 | 22 | 22 |
| Moisture, %≤ | 5.0 | 3.0 | 2.8 | 2.9 | 2.5 |
| Total arsenic (in terms of As), mg/kg≤ | 0.2 | ﹤ 0.2 | ﹤ 0.2 | ﹤ 0.2 | ﹤ 0.2 |
| Lead (in terms of Pb), mg/kg≤ | 0.5 | ﹤ 0.5 | ﹤ 0.5 | ﹤ 0.5 | ﹤ 0.5 |
| Aflatoxin B1 μ g/kg≤ | 5 | ﹤ 5 | ﹤ 5 | ﹤ 5 | ﹤ 5 |
| Saccharin sodium, mg/kg≤ | It must not detect | Do not detect | Do not detect | Do not detect | Do not detect |
| Honey element, mg/kg≤ | It must not detect | Do not detect | Do not detect | Do not detect | Do not detect |
| Sunset yellow | It must not detect | Do not detect | Do not detect | Do not detect | Do not detect |
| Cadmium (in terms of Cd), mg/kg≤ | 0.2 | ﹤ 0.2 | ﹤ 0.2 | ﹤ 0.2 | ﹤ 0.2 |
| Mercury (in terms of Hg), mg/kg≤ | 0.02 | ﹤ 0.02 | ﹤ 0.02 | ﹤ 0.02 | ﹤ 0.02 |
| Chromium (in terms of Cr), mg/kg≤ | 1.0 | ﹤ 1.0 | ﹤ 1.0 | ﹤ 1.0 | ﹤ 1.0 |
As can be seen from Table 1, the indices of chelating amino acids calcium composition of the invention disclosure satisfy that GB 14881
Required with as defined in solid beverage examination of production licence detailed rules and regulations standard.
2nd, to the zoopery of the curative effect of osteoporosis
The preparation of Ovariectomized Female Rats osteoporosis model：Wistar kind 190~210g of female rats body weight, sterile excision
Bilateral ovaries, feed low calcium feed and drink deionized water 30 days, cause osteoporosis model.Normal control rat is done evil through another person
Art.
Experiment：Ovariectomized female rats 30 are taken, three big groups, every group 10 are randomly divided into.First group is placebo, takes peace
Console agent；Second group of compound amino acid chelate calcium group for being produced by the step of embodiment 1 (2), day dosing 4g/kg, (calcium content was suitable
In 1.5g/kg compound amino acid chelate calcium)；The chelating amino acids calcium composition of 3rd group of embodiments of the invention 1,3,6,8
Group, day dosing 4g/kg (compound amino acid chelate calcium of the calcium content equivalent to 1.5g/kg)；4th group is Normal group, is selected
Carry out sham-operation and do not extract the rats of bilateral ovaries.
Raise：22 ± 1 DEG C of laboratory temperature, feeds low calcium feed, drinks deionized water, and Normal group feeds common raise
Material and drinking water.
Animal used above gathered excrement sample in experiment 3 months, and sacrificed by exsanguination takes blood system to be used for blood Ca, P, AKP from serum
Measure；Rats with bilateral femur is peeled off, low temperature refrigerator freezes, for analysis of experiments.
Detection method：
Excrement calcium uses permanganimetric method.
The measure of serum Ca, P and AKP：Serum calcium methyl thymol blue colorimetric method for determining；Serum paraoxonase single agents
Method is determined；Serum alkaline phosphatase (AKP) is determined with 4-NPP method.
Bone densitometry：The right side femur that low temperature refrigerator freezes is taken, femur center 50% is determined and near using borne densitometers
The bone mineral content content at 20% is held, sweep speed is 0.25mm/s, and accuracy is 99%.Unit is contained bone ore deposit every square centimeter
Salt bone (BMC/cm2).
The femur of the fresh stripping in right side is taken, length is surveyed, claims weight in wet base, toast 5 hours, weighed after constant weight in 120 DEG C of baking ovens
As dry weight, then places into 800 DEG C of Muffle furnaces and burns 6 hours, weighed after constant weight as ash content, is surveyed with EDTA compleximetries
Determine the calcium salt content in ash content.
Experimental result is as follows：
Influence of the table 2 to the apparent absorptivity of calcium
| Group | Take in calcium, mg/d | Excrement calcium, mg/d | Apparent absorptivity, % |
| Compound amino acid chelate calcium group | 68±7.2 | 26.3±2.71 | 61.3±3.4 |
| The composition group of embodiment 1 | 66±5.9 | 14.4±1.66 | 78.2±2.9 |
| The composition group of embodiment 3 | 67±5.3 | 13.8±1.50 | 79.4±2.7 |
| The composition group of embodiment 6 | 68±5.1 | 14.1±1.66 | 79.3±2.9 |
| The composition group of embodiment 8 | 69±5.0 | 13.9±1.46 | 79.9±2.8 |
Statistics shows, chelating amino acids calcium composition group of the invention and placebo, compound amino acid calcium group comparing difference
Significantly, P<0.05.
Table 3 to serum calcium, phosphorus, alkaline phosphatase (AKP) influence
Statistics shows, chelating amino acids calcium composition group of the invention and placebo, compound amino acid calcium group comparing difference
Significantly, P<0.05.
Influence of the table 4 to bone density
| Group | Proximal bone bone density (g/cm2) | Bone midpoint bone density (g/cm2) |
| Placebo | 0.227±0.031 | 0.208±0.025 |
| Compound amino acid chelate calcium group | 0.272±0.024 | 0.246±0.015 |
| The composition group of embodiment 1 | 0.292±0.011 | 0.264±0.022 |
| The composition group of embodiment 3 | 0.292±0.011 | 0.264±0.022 |
| The composition group of embodiment 6 | 0.292±0.011 | 0.264±0.022 |
| The composition group of embodiment 8 | 0.292±0.011 | 0.264±0.022 |
| Normal group | 0.289±0.019 | 0.265±0.026 |
Statistics shows, chelating amino acids calcium composition group of the invention and placebo, compound amino acid calcium group comparing difference
Significantly, P<0.05.
Influence of the table 5 to femur weight and calcium content of bone
| Group | Femur weight (g) | Calcium content of bone (mg/g) |
| Placebo | 0.638±0.057 | 221.4±25.3 |
| Compound amino acid chelate calcium group | 0.784±0.034 | 265.7±30.1 |
| The composition group of embodiment 1 | 0.912±0.045 | 298.8±20.4 |
| The composition group of embodiment 3 | 0.928±0.035 | 293.8±18.4 |
| The composition group of embodiment 6 | 0.935±0.041 | 295.3±19.4 |
| The composition group of embodiment 8 | 0.942±0.045 | 291.8±18.2 |
| Normal group | 0.907±0.056 | 299.1±22..3 |
Chelating amino acids calcium composition group and placebo, compound amino acid calcium it can be seen that the present invention are contrasted by upper table 5
Group comparing difference is notable, P<0.05.
3rd, oral administration safety toxicological test
The composition of the present invention is tested according to conventional security toxicological test method, it is as a result as follows：
(1) acute toxicity test
The oral maximum tolerated dose MTD to the acute oral of female small white mouse of the present composition is all higher than 20000mg/
kg。
(2) micronucleus test result：It is negative.
(3) spermatic aberration test result：It is negative.
(4) Salmonella reversion test result：It is negative.
Claims (1)
1. a kind of chelating amino acids calcium composition, it is characterised in that said composition is made up of the raw material of following proportion by weight：
It by hydroxyproline and proline according to mass ratio is 1 that above-mentioned proline composition, which is,：2 are made；
The preparation method of above-mentioned chelating amino acids calcium composition is comprised the steps of：
(1) coarse crushing after fresh ox bone is cleaned, degreasing, boiling seasonings ox bone and distilled water are carried out to ox bone using autoclaving method
Volume ratio be 1：5, temperature is 121 DEG C, and the time is 1.5h；
(2) the fresh ox bone after boiling is drained into profit, it is evenly laid out on square position, in the baking oven for being put into 80 DEG C, 10h drying, warp
Multi-stage crushing, crosses 80 mesh sieves and obtains bovine bone powder, be in mass ratio 1：10 amount adds the salt that concentration is 2mol/L into bovine bone powder
Acid, acidolysis 1.5h at 70 DEG C, suction filtration obtains the supernatant containing solubility calcium, NaOH regulation supernatant pH to 8.0 is added, by compound
Amino acid and Ca2+Mass ratio be 3：1 amount, which is added, reacts 70min at compound amino acid, 80 DEG C, compound amino acid is obtained after drying
Chelate calcium；
(3) compound amino acid chelate calcium 40g, vitamin D 0.0005g, vitamin K 0.005g, glycine are weighed according to the above ratio
30g, hydroxyproline 10g, proline 20g, Bifidobacterium 10g, Huai Goat 15g and chondroitin sulfate 15g, it is fully mixed
Close, chelating amino acids calcium composition is made.
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