CN104388302A - Reaction box for sperm chromatin diffusion experiment - Google Patents
Reaction box for sperm chromatin diffusion experiment Download PDFInfo
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- CN104388302A CN104388302A CN201410723088.XA CN201410723088A CN104388302A CN 104388302 A CN104388302 A CN 104388302A CN 201410723088 A CN201410723088 A CN 201410723088A CN 104388302 A CN104388302 A CN 104388302A
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- rectangle frame
- sperm chromatin
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a reaction box for sperm chromatin diffusion experiment. The reaction box comprises a box body, wherein a box cover is arranged above the box body; six rectangular frames are arranged inside the box body; a reaction groove is formed in the top of each rectangular frame; a heat conduction aluminum module is arranged inside each rectangular frame; the size of the box body is 220mm*120mm*15mm in length, width and height; the thickness of the wall is 1.5mm; the six rectangular frames are arranged in parallel; the size of the rectangular frames is 56mm*29mm*14.5mm in length, width and height; the thickness of the frame is 0.3-1mm; the distance between adjacent rectangular frames is 5mm; the size of each reaction groove is 54mm*27mm*13mm in length, width and height; the thickness of the bottom of each reaction groove is 0.3mm; the size of each heat conduction aluminum module is 54mm*27mm*13mm in length, width and height. The reaction box for sperm chromatin diffusion experiment, which is disclosed by the invention, is simple and smart in structure, convenient and flexible to use, good in use effect, and very applicable to sperm chromatin diffusion experiment.
Description
Technical field
The present invention relates to a kind of reaction box for Sperm Chromatin diffusion test, belong to technical field of medical instruments.
Background technology
Sperm Chromatin diffusion test is a kind of method detecting sperm DNA integrity grown up in recent years, the slide glass that this requirement of experiment is loaded with semen sample under 22 ~ 28 DEG C of constant temps through sex change, cracking, termination reaction, the steps such as 3 step dehydrations, need through the process of 6 kinds of differential responses liquid, key problem in technology be all should immerse completely in reaction solution under horizontality in steps and carry out, and unlike in the vertical insertion reaction liquid of slide glass in routine pathology test, common glass slide dyeing box also designs according to the vertical needs inserted, conventional slide glass specification is 76 × 26 × 1mm.
In horizontality, slide glass to be immersed reaction solution and complete test, also there is no custom-designed reaction colouration box at present, usually the method taked has two kinds: one is by slide glass horizontal positioned, directly reaction solution or staining fluid are dripped and realize on slide glass, be difficult to the amount controlling to drip liquid like this, particularly the cleavage step of Sperm Chromatin diffusion test needs 30 minutes, as direct dropping, affected by environment larger between the reaction period, easily cause the unstable of test conditions, and then cause the unstable of result.Another kind looks for an energy level to put into the colouration box of slide glass, put into relatively large reaction solution, slide glass is placed in one, the method can allow reaction conditions relatively stable, but the amount of required reaction solution is larger, for about 20ml, cost improves greatly, and the pollution that excessive use chemical reagent causes also corresponding increase.
Although Sperm Chromatin diffusion test can complete in room temperature, for realizing operational standardization, temperature of reaction constant is stablized most important for experimental result.In reaction box design can the device of control temperature very necessary.
Summary of the invention
For above-mentioned prior art, the invention provides a kind of reaction box for Sperm Chromatin diffusion test, it is simple, easy to operate, and can reduce reagent dosage.
The present invention is achieved by the following technical solutions:
A kind of reaction box for Sperm Chromatin diffusion test, comprise box body, lid is provided with above box body, tray interior is provided with 6 rectangle frames, each rectangle frame top is provided with reactive tank, each rectangle frame inside is provided with heat conduction aluminum molding block (directly can contact with external Thermostatic platform, to reach the object of constant temperature).
Preferably, described box body is of a size of 220mm (length) × 120mm (wide) × 15mm (height), the thickness of box wall is 1.5mm, 6 rectangle frame flat pattern arrangements, rectangle frame is of a size of 56mm (length) × 29mm (wide) × 14.5mm (height), the thickness of framework is 0.3 ~ 1mm, and the spacing of adjacent rectangle frame is 5mm; Reactive tank is of a size of 54mm (length) × 27mm (wide) × 1.3mm (degree of depth), can carry the reaction solution of 2ml; The thickness of the bottom land of reactive tank is 0.3mm; Heat conduction aluminum molding block is of a size of 54mm × 27mm × 13mm.
Preferably, the material of described box body, lid, rectangle frame, reactive tank is ABS plastic (acrylonitrile-butadiene-styrene (ABS)) (material of reactive tank bottom land can be heat-conducting plastic, to obtain better heat transfer efficiency); The material of described heat conduction aluminum molding block is metallic aluminium.
Preferably, described lid is provided with handle, so that open box body.
Reaction box for Sperm Chromatin diffusion test of the present invention, during use, reaction solution (2ml) is added in reactive tank, then the slide glass being loaded with sperm sample there is the face contact reacts liquid of sample, lie in a horizontal plane in reactive tank, sample is fully immersed in reaction solution in horizontality.Because reactive tank internal diameter is compared with the wide 1mm of slide glass, not easily bubble is produced between reaction solution and slide glass, sample is fully contacted with reaction solution (under can ensureing slide glass horizontality, sample immerses Sperm Chromatin diffusion test reaction solution completely when reaction solution is a small amount of as far as possible, can also ensure that unnecessary reaction solution flows into waste liquid tank).Rectangle frame inside is provided with heat conduction aluminum molding block, can directly contact with external Thermostatic platform (instead of being connected with thermostat), be convenient to heat conduction, make reaction box light easy to operate, ensure that reaction is carried out (adding a cover during reaction at a constant temperature, reduce the volatilization of reaction solution), be convenient to the stdn of warranty test process.A slide glass sample according to reaction requirement, can complete according to step and moves to No. 6 reactive tanks from No. 1 reactive tank.Because the length of reactive tank is short compared with slide glass, then the exposed region outside of slide glass can, as numbering area, not have reaction solution to contact, and makes mobile slide glass easily very convenient.Reaction box for Sperm Chromatin diffusion test of the present invention, simple and ingenious structure, easy to use and flexible, result of use is good, is highly suitable for Sperm Chromatin diffusion test (also can be used for the test that other is similar).
Accompanying drawing explanation
Fig. 1: the structural representation (overlooking) of the reaction box for Sperm Chromatin diffusion test of the present invention.
Fig. 2: the structural representation (square section) of the reaction box for Sperm Chromatin diffusion test of the present invention.
Wherein, 1, box body; 2, lid; 3, rectangle frame; 4, reactive tank; 5, heat conduction aluminum molding block; 6, handle.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
A kind of reaction box for Sperm Chromatin diffusion test, comprise box body 1, as shown in Figure 1 and Figure 2, lid 2 is provided with above box body 1, box body 1 inside is provided with 6 rectangle frames 3, each rectangle frame 3 top is provided with reactive tank 4, each rectangle frame 3 inside is provided with heat conduction aluminum molding block 5 and (directly can contacts with external Thermostatic platform, to reach the object of constant temperature) (rectangle frame is bottomless rectangle frame, namely be hollow bottom rectangle frame, be not the box wall of box body, therefore heat conduction aluminum molding block can directly contact with external Thermostatic platform).
Described box body 1 is of a size of 220mm (length) × 120mm (wide) × 15mm (height), the thickness of box wall is 1.5mm, 6 rectangle frame flat pattern arrangements, rectangle frame 3 is of a size of 56mm (length) × 29mm (wide) × 14.5mm (height), the thickness of framework is 0.3mm, and the spacing of adjacent rectangle frame 3 is 5mm; Reactive tank 4 is of a size of 54mm (length) × 27mm (wide) × 1.3mm (degree of depth), can carry the reaction solution of 2m1; The thickness of the bottom land of reactive tank is 0.3mm; Heat conduction aluminum molding block 5 is of a size of 54mm × 27mm × 13mm.
The material of described box body 1, lid 2, rectangle frame 3, reactive tank 4 can be all ABS plastic (acrylonitrile-butadiene-styrene (ABS)); The material of described heat conduction aluminum molding block is metallic aluminium.
Described lid 2 is provided with handle 6, so that open box body 1.
Reaction box for Sperm Chromatin diffusion test of the present invention, during use, reaction solution (2ml) is added in reactive tank, then the slide glass being loaded with sperm sample there is the face contact reacts liquid of sample, lie in a horizontal plane in reactive tank, sample is fully immersed in reaction solution in horizontality.Because reactive tank internal diameter is compared with the wide 1mm of slide glass, not easily bubble is produced between reaction solution and slide glass, sample is fully contacted with reaction solution (under can ensureing slide glass horizontality, sample immerses Sperm Chromatin diffusion test reaction solution completely when reaction solution is a small amount of as far as possible, can also ensure that unnecessary reaction solution flows into waste liquid tank).Rectangle frame inside is provided with heat conduction aluminum molding block, can directly contact with external Thermostatic platform (instead of being connected with thermostat), make reaction box light easy to operate, ensure that reaction is carried out (adding a cover during reaction at a constant temperature, reduce the volatilization of reaction solution), be convenient to the stdn of warranty test process.A slide glass sample according to reaction requirement, can complete according to step and moves to No. 6 reactive tanks from No. 1 reactive tank.Because the length of reactive tank is short compared with slide glass, then the exposed region outside of slide glass can, as numbering area, not have reaction solution to contact, and makes mobile slide glass easily very convenient.
Claims (4)
1. for a reaction box for Sperm Chromatin diffusion test, it is characterized in that: comprise box body, be provided with lid above box body, tray interior is provided with 6 rectangle frames, and each rectangle frame top is provided with reactive tank, and each rectangle frame inside is provided with heat conduction aluminum molding block.
2. the reaction box for Sperm Chromatin diffusion test according to claim 1, it is characterized in that: described box body is of a size of 220mm × 120mm × 15mm, the thickness of box wall is 1.5mm, 6 rectangle frame flat pattern arrangements, rectangle frame is of a size of 56mm × 29mm × 14.5mm, the thickness of framework is 0.3 ~ 1mm, and the spacing of adjacent rectangle frame is 5mm; Reactive tank is of a size of 54mm × 27mm × 1.3mm, and the thickness of the bottom land of reactive tank is 0.3mm; Heat conduction aluminum molding block is of a size of 54mm × 27mm × 13mm.
3. the reaction box for Sperm Chromatin diffusion test according to claim 1, is characterized in that: described box body, lid, rectangle frame, reactive tank material are ABS plastic; The material of described heat conduction aluminum molding block is metallic aluminium.
4. the reaction box for Sperm Chromatin diffusion test according to claim 1, is characterized in that: described lid is provided with handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410723088.XA CN104388302B (en) | 2014-12-03 | 2014-12-03 | Reaction box for sperm chromatin diffusion test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410723088.XA CN104388302B (en) | 2014-12-03 | 2014-12-03 | Reaction box for sperm chromatin diffusion test |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104388302A true CN104388302A (en) | 2015-03-04 |
| CN104388302B CN104388302B (en) | 2016-08-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410723088.XA Expired - Fee Related CN104388302B (en) | 2014-12-03 | 2014-12-03 | Reaction box for sperm chromatin diffusion test |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319251A (en) * | 2008-05-28 | 2008-12-10 | 山东省计划生育科学技术研究所 | Method for detecting spermatozoon DNA fragment with spermatozoon chromatin diffusion experiment |
| CN201392428Y (en) * | 2009-05-07 | 2010-01-27 | 李西营 | Novel sperm detection glass slide |
| CN102051413A (en) * | 2010-11-10 | 2011-05-11 | 谌兵来 | Human sperm DNA fragment detection method and detection kit |
| CN202330252U (en) * | 2011-09-19 | 2012-07-11 | 南宁松景天伦生物科技有限公司 | Special disposable computer scanning sperm counting board |
| CN104073555A (en) * | 2014-05-14 | 2014-10-01 | 深圳市博锐德生物科技有限公司 | Sperm DNA fragmentation detection kit |
| CN204342768U (en) * | 2014-12-03 | 2015-05-20 | 张丽红 | For the reaction box of Sperm Chromatin diffusion test |
-
2014
- 2014-12-03 CN CN201410723088.XA patent/CN104388302B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101319251A (en) * | 2008-05-28 | 2008-12-10 | 山东省计划生育科学技术研究所 | Method for detecting spermatozoon DNA fragment with spermatozoon chromatin diffusion experiment |
| CN201392428Y (en) * | 2009-05-07 | 2010-01-27 | 李西营 | Novel sperm detection glass slide |
| CN102051413A (en) * | 2010-11-10 | 2011-05-11 | 谌兵来 | Human sperm DNA fragment detection method and detection kit |
| CN202330252U (en) * | 2011-09-19 | 2012-07-11 | 南宁松景天伦生物科技有限公司 | Special disposable computer scanning sperm counting board |
| CN104073555A (en) * | 2014-05-14 | 2014-10-01 | 深圳市博锐德生物科技有限公司 | Sperm DNA fragmentation detection kit |
| CN204342768U (en) * | 2014-12-03 | 2015-05-20 | 张丽红 | For the reaction box of Sperm Chromatin diffusion test |
Non-Patent Citations (2)
| Title |
|---|
| DONALD P. EVENSON, REGINA WIXON: "Clinical aspects of sperm DNA fragmentation detection and male infertility", 《THERIOGENOLOGY》, vol. 65, no. 5, 19 October 2005 (2005-10-19), pages 979 - 991 * |
| 邱毅: "精子染色质扩散实验检测男性不育患者精子DNA碎片", 《国际生殖健康/计划生育杂志》, vol. 27, no. 6, 30 November 2008 (2008-11-30), pages 387 - 389 * |
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| CN104388302B (en) | 2016-08-24 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Qiuju Inventor after: Wang Hongyan Inventor after: Zhang Lihong Inventor after: Liu Bei Inventor after: Zhang Yanping Inventor after: Xu Guanzhao Inventor after: Zhang Wei Inventor after: Zhang Meihua Inventor after: Wang Linlin Inventor after: Jiang Baohong Inventor before: Zhang Lihong Inventor before: Liu Bei Inventor before: Zhang Yanping Inventor before: Xu Guanzhao Inventor before: Zhang Wei Inventor before: Zhang Meihua Inventor before: Wang Linlin Inventor before: Jiang Baohong Inventor before: Wang Hongyan |
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Effective date of registration: 20160628 Address after: 261011 Weifang maternal and child health care hospital, Qingnian Road 76, Weicheng District, Weifang, Shandong Applicant after: Wang Qiuju Applicant after: Zhang Lihong Address before: Central Yuhan road Ji'nan City, Shandong province 250002 City No. 69 Applicant before: Zhang Lihong |
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Granted publication date: 20160824 Termination date: 20161203 |