CN203981612U - A kind of solution example wriggling experimental system - Google Patents
A kind of solution example wriggling experimental system Download PDFInfo
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- CN203981612U CN203981612U CN201420051207.7U CN201420051207U CN203981612U CN 203981612 U CN203981612 U CN 203981612U CN 201420051207 U CN201420051207 U CN 201420051207U CN 203981612 U CN203981612 U CN 203981612U
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Abstract
The utility model provides a kind of solution example wriggling experimental system, comprising: sample vermiculator, and it temperature-controlling chamber and one that comprises that an inside is full of constant temperature circulating liquid is arranged on the sample chamber of described temperature-controlling chamber inside; For holding the solution example sample temporary room of radioactive waste liquid side by side, it is communicated with the lower end of described sample chamber; Receive and according to the conditioning signal of a peripheral input, regulate the two dimension of the position of described sample temporary room to adjust platform; At least one is for holding the soda liquor container of cleaning fluid; And multi-ported valve door syringe pump, it comprises that a valve and is connected to the syringe pump of described valve, wherein, described valve comprises the first valve port being communicated with the upper end of described sample chamber and the 3rd valve port being communicated with described soda liquor container.The utility model can be realized the functions such as solution example auto injection, automatic sample-changing, sample chamber clean automatically, sample wriggling, overcomes the defects such as operation inconvenience in prior art, efficiency is low, meets the application demand of automation mechanized operation.
Description
Technical field
The utility model relates to a kind of solution example wriggling experimental system, belongs to synchrotron radiation field.
Background technology
The structure of biomacromolecule (being mainly protein) and the relation of function are one of current molecular biological key problems.Utilize synchrotron radiation Advance of Small-angle X-ray Scattering Technique, people can study by the sample within the scope of from several KDa to several MDa molecular weight under solution state, and obtain its structural information.This is just for character, conformation change and the protein molecular of research protein molecular under physiological status and the combination of other substrates provide powerful.Therefore, synchrotron radiation Advance of Small-angle X-ray Scattering Technique has received increasing concern in recent years with its unique advantage.
Along with the arrival of genome times afterwards comprehensively, a large amount of proteomics researches become focus.Conventional synchrotron radiation small angle X ray scattering need to configure multiple variable concentrations solution for same protein sample, with this, measures to reduce to interfere and the concentration effect that may exist between particle.In addition, the change of the solution environmental such as damping fluid, pH, ionic strength and adjuvant is the problem that researcher is concerned about on the impact of protein structure and function too.Singularity due to synchrotron radiation experiment, after optical gate is opened, experimenter can not closely control sample, yet, protein example in the face of a large amount of different solutions environment, how realizing sample operation robotization and improve conventional efficient, is the common issue that all synchrotron radiation X-ray small angle experiment centres face.
At present, there is in the world commercial biology laboratory sample introduction equipment, but for synchrotron radiation experiment centre real needs and layout, need to carry out the transformation of hardware and software, be difficult to realize.And domesticly not yet there is a corresponding product.
Subject name is a kind of solution example wriggling experimental provision (utility model patent number: ZL2013 20203183.8; Application for a patent for invention number: patent 201310139099.9) provides a kind of operation experimental system of wriggling easily, but this system can not realize automatic cleaning and auto injection function, thereby the application has carried out upgrading on the basis of this patent.
Utility model content
For the defect existing in prior art, the purpose of this utility model is to provide a kind of solution example wriggling experimental system that can automatically clean auto injection, to realize solution example auto injection, automatic sample-changing, sample chamber, automatically clean, the function such as sample wriggling, effectively overcome the defects such as in prior art, operation is inconvenient, efficiency is low, meet the application demand of synchrotron radiation experiment centre solution example automation mechanized operation.
For achieving the above object, the utility model is by the following technical solutions:
A solution example wriggling experimental system, this experimental system comprises:
Sample vermiculator, it temperature-controlling chamber and one that comprises that an inside is full of constant temperature circulating liquid is arranged on the sample chamber of described temperature-controlling chamber inside;
For holding the solution example sample temporary room of radioactive waste liquid side by side, it is communicated with the lower end of described sample chamber;
Receive and according to the conditioning signal of a peripheral input, regulate the two dimension of the position of described sample temporary room to adjust platform;
At least one is for holding the soda liquor container of cleaning fluid; And
Multi-ported valve door syringe pump, it comprises that a valve and is connected to the syringe pump of described valve, wherein, described valve comprises the first valve port being communicated with the upper end of described sample chamber and the 3rd valve port being communicated with described soda liquor container, described syringe pump receives and according to the control signal of a peripheral input, the described solution example in described sample temporary room is drawn in described sample chamber and it is wriggled back and forth, or the described cleaning fluid suction Nei Yidui sample chamber, sample chamber in described soda liquor container is cleaned.
Further, described sample chamber comprises successively a upper conduit, a kapillary and the downcomer being communicated with, and the upper end of described upper conduit is communicated with described the first valve port, and described downcomer is communicated with described sample temporary room.
Further, described sample temporary room is provided with for holding the sample hole slot of solution example and for discharging the waste liquid hole slot of waste liquid, and described sample hole slot around and described waste liquid hole slot be full of constant temperature circulating liquid around.
Preferably, described wriggling experimental system also comprises a temperature control circulating device being communicated with described temperature-controlling chamber and described sample temporary room.
Preferably, described temperature control circulating device is low temperature thermostat bath.
Further, described sample temporary room one side is provided with the first entrance and the first outlet, described temperature-controlling chamber upper end is provided with the second entrance and the second outlet, wherein, the outlet of described temperature control circulating device is communicated with described the first entrance, described the first outlet is communicated with described the second entrance, and described the second outlet is communicated with the entrance of described temperature control circulating device.
Further, on the cell wall of described waste liquid hole slot one side and apart from one section of At The Height of bottom land of this waste liquid hole slot, be provided with a waste liquid outlet, described waste liquid outlet is connected with a peripheral waste fluid container by a connecting duct.
Aforementioned a kind of solution example wriggling experimental system, described two dimension is adjusted platform and is comprised horizontal level adjustment module and the upright position adjustment module for driving described sample temporary room vertically to move for driving described sample temporary room to move horizontally.
Further, described horizontal level adjustment module comprises that a horizontal base, is arranged on the cross sliding clock and that the horizontal screw lead, on described horizontal base is arranged on described horizontal screw lead and is connected and receives the horizontal stepper motor of described conditioning signal with described horizontal screw lead;
Described upright position adjustment module comprises that one is fixedly connected on the vertical pedestal on described cross sliding clock, and one is arranged on the vertical slipper and that the down-feed screw, on described vertical pedestal is arranged on described down-feed screw is connected and receives the vertical stepper motor of described conditioning signal with described down-feed screw.
Aforementioned a kind of solution example wriggling experimental system, described valve also comprises the second valve port with air communication.
The solution example wriggling experimental system that can automatically clean automatic sample-changing provided by the utility model has following beneficial effect with respect to prior art:
(1) this device is simple to operate, and conventional efficient is high;
(2) adopt high precision multi-ported valve door syringe pump, each valve port by conduit with hold different types of soda liquor container and be communicated with, can, for protein solution sample of different nature, design different cleaning processes;
(3) adopt two dimension to adjust platform, by the adjusting in horizontal and vertical directions, realize the evolution of protein solution sample;
(4) this device is provided with the waste liquid hole slot of special construction, when cleaning fluid waste liquid is discharged, can clean sample chamber downcomer mouth of pipe outer wall, has effectively avoided the cross pollution of different solutions sample room.
(5) by being connected in series with temperature control circulating device, the sample in sample chamber and sample temporary room hole slot all remains under steady temperature, has realized the controlled SAXS of temperature and has measured;
(6), under the accurate control of peripheral computer, multi-ported valve door syringe pump and two dimension are adjusted platform and are cooperatively interacted, and have realized automatic sample-changing, auto injection, and sample chamber is automatically cleaned, is dried the functions such as sample wriggling;
(7) correlation control unit that this device adopts and driver are that synchrotron radiation is special-purpose, meet synchrotron radiation experiment centre real needs and layout, become one with experiment centre miscellaneous equipment.
Accompanying drawing explanation
The accompanying drawing made from reference to conduct exemplary embodiments of the present utility model subsequently, this explanation will be easier to understand, but should not be construed as the restriction to the utility model scope.
Fig. 1 is for can automatically cleaning the structural representation of the solution example wriggling experimental system of automatic sample-changing;
Fig. 2 is the structural representation of multi-ported valve door syringe pump;
Fig. 3 is the structural representation that two dimension is adjusted platform and sample temporary room;
Fig. 4 is the vertical view of sample temporary room;
Fig. 5 is the structural representation of sample vermiculator;
Fig. 6 is the control method process flow diagram of peripheral computer.
Embodiment
For making further to understand technological means of the present utility model and feature in depth, sincerely coordinate accompanying drawing to give again for example and further illustrate in rear:
As Figure 1-5, the utility model provides a kind of solution example wriggling experimental system that can automatically clean auto injection, for the SAXS measurement of solution example, especially for the SAXS measurement of protein solution sample.Specifically as shown in Figure 1, this experimental system mainly comprises a multi-ported valve door syringe pump 1, a sample vermiculator 4, a loop temperature-control device 6 and the sample temporary room 3 connecting successively, also comprise that one for supporting sample temporary room 3 and regulating the two dimension of the position of sample temporary room 3 to adjust platform 2, wherein, multi-ported valve door syringe pump 1 and two dimension are adjusted platform 2 by peripheral computer 5 controls.
Respectively each device in this experimental system is described in detail below:
As depicted in figs. 1 and 2, multi-ported valve door syringe pump 1 comprises a connected syringe pump 11 and a valve 12, and valve 12 comprises a plurality of valve port 13-17, wherein, the first valve port 13 is communicated with the upper end of sample vermiculator 4, the second valve port 14 and air communication, the 3rd valve port 15,16 and 17 is communicated with the soda liquor container 151,161 and 171 that holds cleaning fluid by conduit respectively.In the present embodiment, by peripheral computer 5, control and be positioned at valve 12 stepper motor (not indicating on figure) below and can realize the switching between each valve port 13-17, and control, be positioned at syringe pump 11 another stepper motor (not indicating on figure) below and can drive the leading screw (not indicating on figure) of syringe pump 11 to realize that high-precision solution sucks and discharge.
Fig. 3 shows the structural representation that two dimension is adjusted platform 2 and sample temporary room 3.As shown in the figure, two dimension is adjusted platform 2 and is comprised horizontal level adjustment module and upright position adjustment module, wherein, horizontal level adjustment module comprises that the horizontal screw lead 26, that a horizontal base 24, is arranged on horizontal base 24 is arranged on the horizontal stepper motor 22 that the cross sliding clock 28 and on horizontal screw lead 26 is arranged on horizontal base 24 right-hand members and is connected with horizontal screw lead 26; Upright position adjustment module comprises that one is fixedly connected on the down-feed screw 25, that the vertical pedestal 23, on cross sliding clock 28 is arranged on vertical pedestal 23 and is arranged on the vertical stepper motor 21 that the vertical slipper 27 and on down-feed screw 25 is arranged on vertical pedestal 23 tops and is connected with down-feed screw 25.When sample temporary room 3 need to move horizontally, by horizontal stepper motor 22, drive horizontal screw lead 24, can drive sample temporary room 3 to move horizontally; When sample temporary room 3 needs vertical movement, by vertical stepper motor 21, drive down-feed screws 25, can drive sample temporary room 3 vertically to move.In the present embodiment, vertical stepper motor 21 and horizontal stepper motor 22 receive the conditioning signal of peripheral computer 5 outputs, and perform an action according to this conditioning signal.
In addition, sample temporary room 3 is provided with a plurality of sample hole slots 33 that hold sample and one for discharging the waste liquid hole slot 34 of waste liquid from its top, and its side is provided with the first outlet 31 and the first entrance 32, described constant temperature circulating liquid by this first gateway 31 and 32 from flow through sample temporary room 3 around sample hole slot 33 and waste liquid hole slot 34 of temperature control circulating device 6, temperature control circulating device 6 is at this preferred existing low temperature thermostat bath.Wherein, on the cell wall of waste liquid hole slot 34 1 sides and apart from one section of At The Height of bottom land of hole slot 34, be provided with a waste liquid outlet 35, and the peripheral waste fluid container 36 that this waste liquid outlet 35 holds waste liquid by a connecting duct and is communicated with.
Known in conjunction with Fig. 3 and Fig. 4, sample hole slot 33 and waste liquid hole slot 34 are all cylindrical, wherein, sample hole slot 33 comprises a plurality of hole slots such as the first sample hole slot 331 and the second sample hole slot 332, sample hole slot 33 is divided into diameter 12mm and two kinds of specifications of 6mm herein, is applicable to respectively hold the centrifuge tube (not shown) of different size.When filling the centrifuge tube of solution example and insert in sample hole slot 33, around the constant temperature circulating liquid of sample hole slot 33, can make the sample temperature in centrifuge tube remain constant.
As shown in Figure 5, structural representation for sample vermiculator 4, it comprises that a temperature-controlling chamber 41 and that is full of constant temperature circulating liquid is arranged on the sample chamber 42 in temperature-controlling chamber 41, wherein, temperature-controlling chamber 41 is preferably made of copper, this is that temperature control effect is remarkable because the heat conductivity of copper is good, and its top is provided with the second entrance 43 and the second outlet 44.Described constant temperature circulating liquid is by this second entrance 43 and the second outlet 44 temperature-controlling chamber 41 that flow through, as shown in Figure 1, the outlet of temperature control circulating device 6 is communicated with the first entrance 32, the first outlet 31 is communicated with the second entrance 43, the second outlet 44 is communicated with the entrance of temperature control circulating device 6, thereby realizes constant temperature circulating liquid circulating between temperature control circulating device 6, sample temporary room 3 and sample vermiculator 4.
In the present embodiment, the sample chamber 42 of being located at temperature-controlling chamber 41 inside is comprised of the upper conduit 421 being communicated with successively, kapillary 422 and downcomer 423, wherein, upper conduit 421 is communicated with the first valve port 13 of multi-ported valve door syringe pump 1, and downcomer 423 is for immersing in the sample hole slot 33 or waste liquid hole slot 34 of sample temporary room 3.In addition, the outstanding extension of sidewall of one Ce Zuo45Cong temperature-controlling chamber 41, and on side seat 45, be also provided with screw hole 451, thereby be convenient to this sample vermiculator 4 by side seat, to be fixed on the sample stage of synchrotron radiation X-ray small angle scattering experiment centre, in like manner, also can fix this device 4 by screw hole is set on base 46.
Fig. 6 shows the control method process flow diagram of peripheral computer 5, and its operating process is: first, start peripheral computer 5; Regulate two dimension to adjust the position of platform 2, so that hold downcomer 423 places that the first sample hole slot 331 of solution example to be measured moves to sample vermiculator 4; Sampling volume and the sample introduction speed of multi-ported valve door syringe pump 1 are set, and the first valve port 13 is made as to sample introduction valve port, start sample introduction operation, so that the sample in sample hole slot 33 is sucked in kapillary 422; Then, start the sample wriggling measurement pattern of multi-ported valve door syringe pump 1, and the beam shutter of opening light beam line, carry out synchrotron radiation experiment measuring; After measurement completes, regulate two dimension to adjust the position of platform 2, so that the second empty sample hole slot 33 moves to downcomer 423 places, to reclaim the solution example of discharging in downcomer 423; Subsequently, again regulate two dimension to adjust the position of platform 2, make waste liquid hole slot 34 move to downcomer 423 places, and reset sample introduction valve port, sampling volume and the sample introduction speed of multi-ported valve door syringe pump 1, then start sample introduction operation, so that the cleaning fluid in soda liquor container 151,161 or 171 is sucked in syringe pump 11; Finally, stock layout volume and the stock layout speed of multi-ported valve door syringe pump 1 is set, and the first valve port 13 is made as to stock layout valve port, start stock layout operation, so that the cleaning fluid in syringe pump 11 is injected to sample vermiculator 4; After cleaning operation completes, cleaning fluid is discharged by waste liquid hole slot 34.
For understanding in more detail workflow of the present utility model, lift following examples auto injection and automatic cleaning process be explained:
In soda liquor container 151, hold deionized water solution; The conduit being connected with the second valve port 14 is placed in air; In the first sample hole slot 331, be inserted with the centrifuge tube that holds testing protein solution example; The second sample hole slot 332 interpolations are free centrifuge tube, give over to and survey protein solution recovery use.
Because downcomer 423 positions of sample vermiculator 4 are fixed, can regulate two dimension to adjust platform 2 by peripheral computer 5, so that the first sample hole slot 331 moves to downcomer 423 places, and guarantee that downcomer 423 mouths of pipe immerse in the testing protein solution in the first sample hole slot 331.Sampling volume and the sample introduction speed of multi-ported valve door syringe pump 1 are set by peripheral computer 5 subsequently, and the first valve port 13 is made as to sample introduction valve port, start after sample introduction operation, syringe pump 11 is drawn into testing protein solution in the kapillary 422 of sample vermiculator 4 from the centrifuge tube of the first sample hole slot 331, then open multi-ported valve door syringe pump 1 sample wriggling measurement pattern so that testing protein solution in kapillary 422, wriggle back and forth, open beam shutter, carry out the protein solution synchrotron radiation small angle X ray scattering of antiradiation injury and measure.After measurement completes, again regulate two dimension to adjust platform 2, so that the second sample hole slot 332 moves to downcomer 423 places, by controlling multi-ported valve door syringe pump 1, the protein solution sample of having surveyed is discharged to from kapillary 422 in the empty centrifuge tube of the second sample hole slot 332, to reclaim, surveys protein solution.
Again regulate two dimension to adjust platform 2, make waste liquid hole slot 34 move to downcomer 423 places.Then sampling volume and the sample introduction speed of multi-ported valve door syringe pump 1 are set, and the 3rd valve port 15 is made as to sample introduction valve port, start after sample introduction operation, syringe pump 11 is drawn into deionized water solution in syringe pump 11 from soda liquor container 151.Stock layout volume and the stock layout speed of multi-ported valve door syringe pump 1 are set, and the first valve port 13 is made as to stock layout valve port, start after stock layout operation, syringe pump 11 is discharged its inner deionized water solution, flow through upper conduit 421, kapillary 422, the downcomer 423 of sample vermiculator 4 of deionized water flow to waste liquid hole slot 34, and finally by waste liquid outlet 35, is expelled in peripheral waste fluid container 36.Because waste liquid outlet 35 has a segment distance apart from the bottom of waste liquid hole slot 34, when downcomer 423 stretches into waste liquid hole slot 34 inside and carries out stock layout, the deionized water that residues in waste liquid hole slot 34 bottoms can clean downcomer 423 mouth of pipe outer walls, has effectively avoided the cross pollution of different solutions sample room.In addition, when the second valve port 14 of multi-ported valve door syringe pump 1 is made as sample introduction valve port, same aforesaid operations, can pass into air in sample vermiculator 4, realizes sample chamber functions/drying.
Should be appreciated that cleaning solution of the present utility model is not limited in deionized water solution, be also not limited only to a kind of cleaning solution, can, for different protein solution samples, design concrete cleaning process.
Above-described, be only preferred embodiment of the present utility model, be not intended to limit scope of the present utility model, above-described embodiment of the present utility model can also make a variety of changes.Be that simple, the equivalence that every claims according to the utility model application and description are done changes and modify, all fall into the claim protection domain of the utility model patent.The utility model not detailed description be routine techniques content.
Claims (8)
1. a solution example wriggling experimental system, is characterized in that, this experimental system comprises:
Sample vermiculator, it temperature-controlling chamber and one that comprises that an inside is full of constant temperature circulating liquid is arranged on the sample chamber of described temperature-controlling chamber inside;
For holding the solution example sample temporary room of radioactive waste liquid side by side, it is communicated with the lower end of described sample chamber;
Receive and according to the conditioning signal of a peripheral input, regulate the two dimension of the position of described sample temporary room to adjust platform;
At least one is for holding the soda liquor container of cleaning fluid; And
Multi-ported valve door syringe pump, it comprises that a valve and is connected to the syringe pump of described valve, wherein, described valve comprises the first valve port being communicated with the upper end of described sample chamber and the 3rd valve port being communicated with described soda liquor container, described syringe pump receives and according to the control signal of a peripheral input, the described solution example in described sample temporary room is drawn in described sample chamber and it is wriggled back and forth, or the described cleaning fluid suction Nei Yidui sample chamber, sample chamber in described soda liquor container is cleaned;
Wherein, described two dimension adjustment platform comprises horizontal level adjustment module and the upright position adjustment module for driving described sample temporary room vertically to move for driving described sample temporary room to move horizontally;
Described horizontal level adjustment module comprises that a horizontal base, is arranged on the cross sliding clock and that the horizontal screw lead, on described horizontal base is arranged on described horizontal screw lead and is connected and receives the horizontal stepper motor of described conditioning signal with described horizontal screw lead;
Described upright position adjustment module comprises that one is fixedly connected on the vertical pedestal on described cross sliding clock, and one is arranged on the vertical slipper and that the down-feed screw, on described vertical pedestal is arranged on described down-feed screw is connected and receives the vertical stepper motor of described conditioning signal with described down-feed screw.
2. solution example wriggling experimental system according to claim 1, it is characterized in that, described sample chamber comprises successively a upper conduit, a kapillary and the downcomer being communicated with, and the upper end of described upper conduit is communicated with described the first valve port, and described downcomer is communicated with described sample temporary room.
3. solution example wriggling experimental system according to claim 1, it is characterized in that, described sample temporary room is provided with for holding the sample hole slot of solution example and for discharging the waste liquid hole slot of waste liquid, and described sample hole slot around and described waste liquid hole slot be full of constant temperature circulating liquid around.
4. solution example wriggling experimental system according to claim 3, is characterized in that, described wriggling experimental system also comprises a temperature control circulating device being communicated with described temperature-controlling chamber and described sample temporary room.
5. solution example wriggling experimental system according to claim 4, is characterized in that, described temperature control circulating device is low temperature thermostat bath.
6. solution example wriggling experimental system according to claim 5, is characterized in that,
Described sample temporary room one side is provided with the first entrance and the first outlet, described temperature-controlling chamber upper end is provided with the second entrance and the second outlet, wherein, the outlet of described temperature control circulating device is communicated with described the first entrance, described the first outlet is communicated with described the second entrance, and described the second outlet is communicated with the entrance of described temperature control circulating device.
7. solution example wriggling experimental system according to claim 3, it is characterized in that, on the cell wall of described waste liquid hole slot one side and apart from one section of At The Height of bottom land of this waste liquid hole slot, be provided with a waste liquid outlet, described waste liquid outlet is connected with a peripheral waste fluid container by a connecting duct.
8. solution example wriggling experimental system according to claim 1, is characterized in that, described valve also comprises the second valve port with air communication.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201420051207.7U CN203981612U (en) | 2014-01-26 | 2014-01-26 | A kind of solution example wriggling experimental system |
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| CN201420051207.7U CN203981612U (en) | 2014-01-26 | 2014-01-26 | A kind of solution example wriggling experimental system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103776854A (en) * | 2014-01-26 | 2014-05-07 | 中国科学院上海应用物理研究所 | Solution sample creep experiment system |
| CN109073664A (en) * | 2016-04-22 | 2018-12-21 | 贝克顿·迪金森公司 | Automatic diagnositc analyser and its operating method |
| US11754582B2 (en) | 2016-04-22 | 2023-09-12 | Becton Dickinson And Company | Automated diagnostic analyzer and method for its operation |
-
2014
- 2014-01-26 CN CN201420051207.7U patent/CN203981612U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103776854A (en) * | 2014-01-26 | 2014-05-07 | 中国科学院上海应用物理研究所 | Solution sample creep experiment system |
| CN109073664A (en) * | 2016-04-22 | 2018-12-21 | 贝克顿·迪金森公司 | Automatic diagnositc analyser and its operating method |
| CN109073664B (en) * | 2016-04-22 | 2022-12-06 | 贝克顿·迪金森公司 | Automated diagnostic analyzer and method of operating the same |
| US11754582B2 (en) | 2016-04-22 | 2023-09-12 | Becton Dickinson And Company | Automated diagnostic analyzer and method for its operation |
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