CN103398890A - Liquid-based cell sheet producing system and liquid-based cell sheet producing method - Google Patents
Liquid-based cell sheet producing system and liquid-based cell sheet producing method Download PDFInfo
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- CN103398890A CN103398890A CN2013103689967A CN201310368996A CN103398890A CN 103398890 A CN103398890 A CN 103398890A CN 2013103689967 A CN2013103689967 A CN 2013103689967A CN 201310368996 A CN201310368996 A CN 201310368996A CN 103398890 A CN103398890 A CN 103398890A
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Abstract
The invention discloses a liquid-based cell sheet producing system which comprises a rack, a sheet producing platform fixed on the rack, a plurality of sheet producing plates fixed on the sheet producing platform, a moving device perpendicularly connected with a bearing frame of the rack, a mechanical arm perpendicularly and slidingly connected with the moving device, a cantilever perpendicularly and slidingly connected with the mechanical arm, a reagent bottle connected with the cantilever by a catheter, and a microcomputer system controlling the moving device, the mechanical arm and the cantilever, wherein a control window is arranged on the rack, and controls the microcomputer system; and the reagent bottle comprises a waste liquid barrel, a flushing fluid reagent bottle part and a buffer fluid reagent bottle part. A liquid-based cell sheet producing method adopts the liquid-based cell sheet producing system. With the adoption of the technical scheme, the defects that a system operation mode is single, a procedure is solidified, a pipeline cannot be flushed automatically, a requirement on the quantity of specimens is strict, flux is low, energy consumption and reagent consumption material loss are great, a failure rate is high, maintenance is difficult, and equipment is costly are overcome.
Description
Technical field
The present invention relates to pathology detection and cell pathology equipment technical field, refer in particular to a kind of liquid-based specimens to the pathology censorship and carry out system and device and the flaking method thereof of automatic film-making.
Background technology
Along with the fast development of pathology technique and the raising of the level of economic development, make second generation liquid-based cell sample manufacturing technology take decanter type liquid-based cell sample manufacturing technology as representative in pathological diagnosis, especially the application in the preparation of cell pathology sample is more and more extensive.Compare with traditional manual smear technique, decanter type liquid-based cell sample manufacturing technology has solved the film-making background subtraction, cell distribution is inhomogeneous, lose the positive critical defect that becomes to grade, there is report to point out,, for the recall rate of cytology positive sample (HISL+), adopt " U.S. surpasses cypress decanter type liquid-based cell sample manufacturing technology (LCT) " to improve 64.4% than traditional-handwork method.And with the liquid-based cell sample manufacturing technology " membrane liquid base thin layer tabletting technology (TCT) " of the first generation, compare, decanter type liquid-based cell sample manufacturing technology also has clear superiority, and for example, the sampling brush brush directly is retained in specimen bottle, can not cause cell loss; Have separation and extraction technology, optionally keep the diagnosis composition; Do not use filter membrane, can not cause filter membrane to stop up, thereby affect the transfer of cell; A film-making multiple, but batch operation; Carry automatic pap staining function etc.
But on domestic and international market, existing decanter type liquid-based cell sample manufacturing equipment and flaking method thereof also exist obvious design defect and unreasonable part, and be specific as follows:
1, nearly all home products, it is when film-making, and same sample once can only film-making 1, and 24 of a collection of maximum film-makings, so just can't meet the user who film-making quantity, efficiency is had higher/specific demand;
When 2, most products all require each film-making, the number of samples amount must be that 4 or 4 multiple is (such as 4,8,12 ...), and every metallochromy pipe on shelf all will connect 4 flexible pipes, each flexible pipe is the different staining reagent of UNICOM respectively, various flexible pipes are more exposed in external environment, easy tied up in knots in work; Easy aging leakage of long duration, form and pollute.Cause complicated operation, reagent and consumptive material waste serious, energy consumption and high cost, the pipeline shortcomings such as easily stifled leakiness, Maintenance Difficulty, serviceable life be short;
3, the most products program design is relatively solidified, single, the demand that can't adapt to multiple personal, often do not possess following functions, as " same sample is made 2 sheets simultaneously ", " same sample is made the slice, thin piece of two variable concentrations simultaneously ", " number of times/frequency that sample mixes ", " concentration adjustment of sample ", " adjusting of settling time ", " pipeline auto-flushing " etc., and these functions all can affect the availability of this system and for the production effect of separate sources sample, especially " same sample is made 2 sheets simultaneously ", " same sample is made the slice, thin piece of two variable concentrations simultaneously " these two functions are for need to be essential concerning sample carries out the user of multinomial joint-detection or comparative study.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of liquid-based cell sample manufacturing system and flaking method thereof, overcome that system operation modes is single, program Solidification, can't the auto-flushing pipeline,, small throughput, energy consumption strict to sample quantity and the loss of reagent consumptive material is large, the defects such as failure rate is high, Maintenance Difficulty, apparatus expensive.
In order to solve the problems of the technologies described above, technical scheme of the present invention is: a kind of liquid-based cell sample manufacturing system, comprise frame, and described frame comprises base plate, perpendicular to the carrier of base plate be located at the protective shield of base plate top perpendicular to carrier; Be fixed on the film-making platform on frame; Be fixed on a plurality of film-making plates on the film-making platform; With the carrier of frame mobile device connected vertically; The mechanical arm that be slidably connected vertical with mobile device; The cantilever that be slidably connected vertical with mechanical arm; The reagent bottle that is connected by conduit with cantilever; Control the microcomputer system of mobile device, mechanical arm and cantilever; Also be provided with the control window on described frame, control the window control microcomputer system, described reagent bottle comprises waste liquid barrel, washing fluid reagent bottle and damping fluid reagent bottle.
Further, be fixed with reagent accumulator tank and transfer pipet recovery approach on described film-making platform, described film-making platform is provided with a plurality of circular ports of rectangular arranged.
Preferably, described circular port quantity is 48.
Further, described mobile device comprises that horizontal guide rail, stop means, gearing and the link block that is connected with mechanical arm, described mechanical arm comprise body, are arranged on horizontal guide rail, gearing and the contiguous block that is connected with cantilever in body.
further, described cantilever comprises the cantilever body, discharge mechanism, upright guide rail, sliding block, photoelectric induction device, the liquid feeding sleeve, irrigation sleeve, stepper motor and jacking gear, described cantilever body comprises the first plane and the second plane, described discharge mechanism, upright guide rail, photoelectric induction device and liquid feeding sleeve are fixed on the first plane, described irrigation sleeve, stepper motor and jacking gear are fixed on the second plane, described liquid feeding sleeve is by sliding block vertical connecting guide rail, described irrigation sleeve connects jacking gear, described stepper motor has two, difference vertical connecting guide rail and jacking gear, the lower end of described liquid feeding sleeve is provided with the first needle tubing and the second needle tubing, the lower end of described irrigation sleeve is provided with the 3rd needle tubing and the 4th needle tubing, described the second needle tubing connects waste liquid barrel with the 3rd needle tubing by conduit, described the first needle tubing connects the damping fluid reagent bottle by conduit, and described the 4th needle tubing connects the washing fluid reagent bottle by conduit.
Further, the Length Ratio first short 0.2cm-1cm of needle tubing of described the second needle tubing, the 4th needle tubing is than the 3rd short 0.5cm-2cm of needle tubing length, the cross section of described the 3rd needle tubing and surface level have the angle of 30 °-60 °, described the 4th needle tubing lower end is crooked laterally, and angle of bend is 30 °-60 °.
Preferably, the Length Ratio first short 0.5cm of needle tubing of described the second needle tubing, the 4th needle tubing is than the 3rd short 18.cm of needle tubing length, and the cross section of described the 3rd needle tubing and surface level have the angle of 45 °, described the 4th needle tubing lower end is crooked laterally, and angle of bend is 45 °.
Further, described film-making plate is fixed on the film-making platform by securing member, and described film-making plate is provided with a plurality of slide grooves that are arranged in parallel, and the middle part of described slide groove is provided with a pair of chute.
Further, a plurality of control programs of storage in described microcomputer system, described control window adopts touch manner to control, multilevel menu, first order menu comprises that switch control, parameter setting, operation controls, number of samples control, programmed control and time on date controls, and Level-2 menu comprises that amount of buffer controls, takes out that specimen amount is controlled, flushing fluid is controlled, the settling time is controlled, mix number of times controls and mix liquid absorption control.
A kind of liquid-based cell sample manufacturing method, adopt above-mentioned liquid-based cell sample manufacturing system.
A kind of liquid-based cell sample manufacturing method, is characterized in that, comprises the following steps:
Step 1: add cell separation liquid in test tube, then sample is slowly joined in test tube along tube wall, take out supernatant after centrifugal through twice and keep sediment, then with test tube vibrations 5 to 30 seconds;
Step 2: test tube is put into the circular port of film-making platform, then transfer pipet is put into test tube, then the pre-service microslide is put into the slide groove of film-making plate, settling bin is written into the film-making plate, finally the film-making plate is fixed on the film-making platform;
Step 3: at the control window, carry out the program parameter setting, option program is controlled in first order menu, select needed program, select again parameter to control, enter Level-2 menu, amount of buffer be set, take out specimen amount, flushing fluid, settling time, mix number of times and mix liquid absorption, arrange complete after click determine then to get back to first order menu and click operation;
Step 4: under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to the test tube placement direction, cantilever moves to the test tube top by the horizontal guide rail of mechanical arm, liquid feeding sleeve down sliding on cantilever enters test tube and with transfer pipet, is locked, and the first needle tubing of liquid feeding sleeve lower end mixes by the second needle tubing after in vitro adding damping fluid; Mix rear liquid feeding sleeve upward sliding and mention the sample of specified amount by transfer pipet;
Step 5: under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to film-making plate direction, cantilever moves to the settling bin top by the horizontal guide rail of mechanical arm, and transfer pipet discharges the specified amount sample to settling bin, and microcomputer system starts countdown;
Step 6: under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to transfer pipet recovery approach direction, cantilever moves to transfer pipet recovery approach top by the horizontal guide rail of mechanical arm, by the discharge mechanism on cantilever, transfer pipet is laid;
Step 7: after the microcomputer system countdown finishes, under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to film-making plate direction, cantilever moves to the settling bin top by the horizontal guide rail of mechanical arm, jacking gear control irrigation sleeve on cantilever moves down and enters settling bin, the 3rd needle tubing of irrigation sleeve lower end siphons away the supernatant in settling bin, then by the 4th needle tubing, injects the washing fluid of specified amount to settling bin;
Step 8: under microcomputer system was controlled, the 3rd needle tubing of irrigation sleeve lower end siphoned away washing fluid, and then the 4th needle tubing injects the washing fluid of specified amount to settling bin;
Step 9: under microcomputer system was controlled, after repeating step 8 one to three times, microcomputer system entered countdown, and after countdown finishes, the 3rd needle tubing siphons away the washing fluid in settling bin: mobile device, mechanical arm and cantilever automatically reset and stop;
Step 10: take off microslide, be placed in 95% alcohol fixing, film-making is completed.
Further, the cell separation liquid described in step 1 is any in Percoll parting liquid, Ficoll parting liquid.
Further, the damping fluid described in step 4 is any in phosphate buffer, Tris damping fluid.
Further, step 7 is any in 95% ethanol, 100% ethanol, 50% isopropyl alcohol+50% alcohol mixture, 50% isopropyl alcohol+48% ethanol+2% carbinol mixture to the washing fluid described in step 9.
Adopt the beneficial effect of technical scheme of the present invention:
(1) between a collection of 1-48 processed, any amount is opened sample slice, not only eliminated the restriction of like product number of samples requirement for " 4 multiples ", saved resource, consumptive material and reagent cost have been saved, also greatly improve work efficiency, be fit to different scales inspection center, medical diagnosis center, pathology department's use;
(2) procedure Selection is flexible, both can realize that same sample once made 1 sheet, can realize that also same sample once makes 2 sheets, expand the range of application of clinical samples, be particularly suitable for same sample and do simultaneously pathologic applications and the Clinical Comparison Study of multinomial joint-detection;
(3) perfect in shape and function, parameter setting by personalization, make this system more meet clinical actual demand, be no matter that the degree that mixes of sample, the ratio of dilution, the volume of transfer, the time of sedimentation, degree of flushing etc. all can be carried out personalized customization by the parameter setting, guarantee that production effect meets the demand of different user;
(4) touch screen design makes user's operation more quick, easily grasps;
(5) the native system simple structure, easy to operate, with the price of similar domestic and imported product costliness, with complicated system, compares, and cost is cheaper, safeguards conveniently, and stabilization of equipment performance is higher, is more conducive to clinical popularization on a large scale;
(6) flaking method of a kind of liquid-based cell sample manufacturing system involved in the present invention is compared with traditional flaking method, has the following advantages: blood and mucus treatment effect are better, can remove most blood and mucus, are not easy to occur pipeline or filter membrane and stop up; The film-making background is cleaner, can remove most cell fragment, impurity and inflammatory cell, makes background more clear; Cell distribution is more even, the situation of the agglomerating or skewness of cell aggregation seldom occurs; The cell enrichment degree is better, for the cell that diagnostic value is arranged, has better accumulation ability, improves positive rate; Consumptive material and reagent cost are cheap, do not need, do not rely on expensive import consumptive material and reagent.
Description of drawings
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is liquid-based cell sample manufacturing system architecture schematic diagram of the present invention;
Fig. 2 is film-making platform structure schematic diagram in liquid-based cell sample manufacturing system of the present invention;
Fig. 3 is film-making plate front view in liquid-based cell sample manufacturing system of the present invention;
Fig. 4 is mobile device structural representation in liquid-based cell sample manufacturing system of the present invention;
Fig. 5 is mechanical arm structural representation in liquid-based cell sample manufacturing system of the present invention;
Fig. 6 is cantilever front view in liquid-based cell sample manufacturing system of the present invention;
Fig. 7 is cantilever right view in liquid-based cell sample manufacturing system of the present invention;
Fig. 8 is cantilever left view in liquid-based cell sample manufacturing system of the present invention;
Fig. 9 controls window first order menu schematic diagram in liquid-based cell sample manufacturing system of the present invention;
Figure 10 controls window Level-2 menu schematic diagram in liquid-based cell sample manufacturing system of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
A kind of liquid-based cell sample manufacturing system shown in Figure 1, comprise frame 1, and frame 1 comprises base plate 11, perpendicular to the carrier 12 of base plate 11 be located at the protective shield 13 of base plate 11 tops perpendicular to carrier 12; Be fixed on the film-making platform 2 on frame 1; Be fixed on a plurality of film-making plates 3 on film-making platform 2; With the carrier 12 of frame 1 mobile device 4 connected vertically; The mechanical arm that be slidably connected 5 vertical with mobile device 4; The cantilever that be slidably connected 6 vertical with mechanical arm 5; The reagent bottle 7 that is connected by conduit with cantilever 6; Control the microcomputer system of mobile device 4, mechanical arm 5 and cantilever 6; Also be provided with on described frame 1 and control window 8, control window 8 and control microcomputer system, described reagent bottle 7 comprises waste liquid barrel 71, washing fluid reagent bottle 72 and damping fluid reagent bottle 73.
Be fixed with reagent accumulator tank 21 and transfer pipet recovery approach 22 on film-making platform 2 shown in Figure 2, described film-making platform 2 is provided with a plurality of circular ports 23 of rectangular arranged, the diameter of circular hole 23 is between 0.5cm-3cm, quantity is between 1-48, be mainly used in loading test tube in circular port, transfer pipet after use is put into transfer pipet recovery approach 22, and reagent accumulator tank 21 is fixed on film-making platform 2 by securing member, the unnecessary reagent that flows out while being mainly used in reclaiming flushing line.
Fig. 1, film-making plate 3 shown in Figure 3 are provided with a plurality of slide grooves 31 that are arranged in parallel, the middle part of described slide groove 31 is provided with a pair of chute 32, slide groove 31 has 12, one two of rows are arranged in parallel, slide groove 31 is used for loading the pre-service microslide, the settling bin of using during film-making is combined closely by chute 32 and pre-service microslide, and film-making plate 3 is fixing by securing member and film-making platform 2, and detachable.
Mobile device shown in Figure 4 comprises horizontal guide rail 41, stop means 42, gearing 43 and the link block 44 that is connected with mechanical arm 5.
Mechanical arm shown in Figure 5 comprises body 51, is arranged on horizontal guide rail 52, gearing 53 and the contiguous block 54 that is connected with cantilever 6 in body 51.
Fig. 1, Fig. 6, Fig. 7, cantilever shown in Figure 8 comprises cantilever body 61, discharge mechanism 62, upright guide rail 63, sliding block 64, photoelectric induction device 65, liquid feeding sleeve 66, irrigation sleeve 67, stepper motor 68 and jacking gear 69, described cantilever body 61 comprises the first plane 611 and the second plane 612, described discharge mechanism 62, upright guide rail 63, photoelectric induction device 65 and liquid feeding sleeve 66 are fixed on the first plane 611, described irrigation sleeve 67, stepper motor 68 and jacking gear 69 are fixed on the second plane 612, thereby described discharge mechanism 62 contacts the unloading transfer pipet when descending with the outer rim of transfer pipet, described liquid feeding sleeve 66 is by sliding block 64 vertical connecting guide rails 63, slide up and down, the diameter of liquid feeding sleeve 66 lower ends is less than the internal diameter of transfer pipet, can be by location and descending automatic loading transfer pipet, described irrigation sleeve 67 connects jacking gear 69, described stepper motor 68 has two, difference vertical connecting guide rail 63 and jacking gear 69, the lower end of described liquid feeding sleeve 66 is provided with the first needle tubing 661 and the second needle tubing 662, the lower end of described irrigation sleeve 67 is provided with the 3rd needle tubing 671 and the 4th needle tubing 672, described the second needle tubing 662 connects waste liquid barrel 71 with the 3rd needle tubing 671 by conduit, described the first needle tubing 661 connects damping fluid reagent bottle 73 by conduit, described the 4th needle tubing 672 connects washing fluid reagent bottle 72 by conduit, the Length Ratio first short 0.2cm-1cm of needle tubing 661 of described the second needle tubing 662, the 4th needle tubing 672 to the three needle tubing 671 short 0.5cm-2cm of length, the cross section of described the 3rd needle tubing 671 and surface level have the angle of 30 °-60 °, the angle of 45 ° most preferably, adopt this structure can avoid needle tubing transversal section when imbibition, with sample slice, whole the contact occurs, described the 4th needle tubing 672 lower ends are crooked laterally, angle of bend is 30 °-60 °, most preferably 45 °, adopt this structure to make in the tube wall flushing of flushing time delay rather than directly with the hydraulic shock sample slice, avoid cell to fall sheet.
A plurality of control programs of storage in Fig. 9, Figure 10 microcomputer system, described control window adopts touch manner to control, multilevel menu, first order menu comprises switch control, parameter setting, operation control, number of samples control, programmed control and the control of time on date, wherein, the parameter setting contains Level-2 menu, comprises that amount of buffer controls, takes out that specimen amount is controlled, flushing fluid is controlled, the settling time is controlled, mixes number of times and control and mix liquid absorption and control.Select the switch control in first order menu carry out the automated reagent perfusion and rinse catheter interior, select " F+ " to start auto-flushing, selection " F-" stops rinsing; Control by the operation in first order menu, can realize program operation, suspend or stop; Control by the number of samples in first order menu, can realize that number of samples is carried out numerical key to be selected; By the programmed control in first order menu, can realize that program is carried out numerical key to be selected, select " 1 " to represent that same sample once makes 1 sheet, select " 2 " to represent that same sample once makes 2 sheets; , by the control of the time on date in first order menu, can set instant date, time; Control by the amount of buffer in Level-2 menu, can realize that the volume that injects damping fluid is carried out numerical key to be regulated; Control by the specimen amount of taking out in Level-2 menu, can realize the sample volume that different settling bins shift is regulated respectively; Control by the flushing fluid in Level-2 menu, can realize the volume of washing fluid filling is carried out the numerical key adjusting; , by the control of the settling time in Level-2 menu, can realize that the sample settling time is carried out numerical key to be selected; Control by the number of times that mixes in Level-2 menu, the numerical key that mixes number of times in the time of can realizing specimen automatic mixing is regulated; Control by the liquid absorption that mixes in Level-2 menu, can realize that the liquid absorption after specimen automatic mixing is carried out numerical key to be regulated.
A kind of liquid-based cell sample manufacturing method, adopt above-mentioned liquid-based cell sample manufacturing system.
A kind of liquid-based cell sample manufacturing method comprises the following steps:
Step 1: add cell separation liquid in test tube, then sample is slowly joined in test tube along tube wall, take out supernatant after centrifugal through twice and keep sediment, then with test tube vibrations 5 to 30 seconds;
Step 2: test tube is put into the circular port of film-making platform, then transfer pipet is put into test tube, then the pre-service microslide is put into the slide groove of film-making plate, settling bin is written into the film-making plate, finally the film-making plate is fixed on the film-making platform;
Step 3: at the control window, carry out the program parameter setting, option program is controlled in first order menu, select needed program, select again parameter to control, enter Level-2 menu, amount of buffer be set, take out specimen amount, flushing fluid, settling time, mix number of times and mix liquid absorption, arrange complete after click determine then to get back to first order menu and click operation;
Step 4: under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to the test tube placement direction, cantilever moves to the test tube top by the horizontal guide rail of mechanical arm, liquid feeding sleeve down sliding on cantilever enters test tube and with transfer pipet, is locked, and the first needle tubing of liquid feeding sleeve lower end mixes by the second needle tubing after in vitro adding damping fluid; Mix rear liquid feeding sleeve upward sliding and mention the sample of specified amount by transfer pipet;
Step 5: under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to film-making plate direction, cantilever moves to the settling bin top by the horizontal guide rail of mechanical arm, and transfer pipet discharges the specified amount sample to settling bin, and microcomputer system starts countdown;
Step 6: under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to transfer pipet recovery approach direction, cantilever moves to transfer pipet recovery approach top by the horizontal guide rail of mechanical arm, by the discharge mechanism on cantilever, transfer pipet is laid;
Step 7: after the microcomputer system countdown finishes, under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to film-making plate direction, cantilever moves to the settling bin top by the horizontal guide rail of mechanical arm, jacking gear control irrigation sleeve on cantilever moves down and enters settling bin, the 3rd needle tubing of irrigation sleeve lower end siphons away the supernatant in settling bin, then by the 4th needle tubing, injects the washing fluid of specified amount to settling bin;
Step 8: under microcomputer system was controlled, the 3rd needle tubing of irrigation sleeve lower end siphoned away washing fluid, and then the 4th needle tubing injects the washing fluid of specified amount to settling bin;
Step 9: under microcomputer system was controlled, after repeating step 8 one to three times, microcomputer system entered countdown, and after countdown finishes, the 3rd needle tubing siphons away the washing fluid in settling bin: mobile device, mechanical arm and cantilever automatically reset and stop;
Step 10: take off microslide, be placed in 95% alcohol fixing, film-making is completed.
Cell separation liquid described in step 1 is any in Percoll parting liquid, Ficoll parting liquid, damping fluid described in step 4 is any in phosphate buffer, Tris damping fluid, and step 7 is any in 95% ethanol, 100% ethanol, 50% isopropyl alcohol+50% alcohol mixture, 50% isopropyl alcohol+48% ethanol+2% carbinol mixture to the washing fluid described in step 9.
Although in conjunction with preferred embodiment, specifically show and introduced the present invention; but the those skilled in the art should be understood that; within not breaking away from the spirit and scope of the present invention that appended claims limits; in the form and details the present invention is made a variety of changes, be protection scope of the present invention.
Claims (10)
1. liquid-based cell sample manufacturing system is characterized in that: comprise frame, described frame comprises base plate, perpendicular to the carrier of base plate be located at the protective shield of base plate top perpendicular to carrier; Be fixed on the film-making platform on frame; Be fixed on a plurality of film-making plates on the film-making platform; With the carrier of frame mobile device connected vertically; The mechanical arm that be slidably connected vertical with mobile device; The cantilever that be slidably connected vertical with mechanical arm; The reagent bottle that is connected by conduit with cantilever; Control the microcomputer system of mobile device, mechanical arm and cantilever; Also be provided with the control window on described frame, control the window control microcomputer system, described reagent bottle comprises waste liquid barrel, washing fluid reagent bottle and damping fluid reagent bottle.
2. a kind of liquid-based cell sample manufacturing system according to claim 1, it is characterized in that: be fixed with reagent accumulator tank and transfer pipet recovery approach on described film-making platform, described film-making platform is provided with a plurality of circular ports of rectangular arranged.
3. a kind of liquid-based cell sample manufacturing system according to claim 1, it is characterized in that: described mobile device comprises that horizontal guide rail, stop means, gearing and the link block that is connected with mechanical arm, described mechanical arm comprise body, are arranged on horizontal guide rail, gearing and the contiguous block that is connected with cantilever in body.
4. a kind of liquid-based cell sample manufacturing system according to claim 1, it is characterized in that: described cantilever comprises the cantilever body, discharge mechanism, upright guide rail, sliding block, photoelectric induction device, the liquid feeding sleeve, irrigation sleeve, stepper motor and jacking gear, described cantilever body comprises the first plane and the second plane, described discharge mechanism, upright guide rail, photoelectric induction device and liquid feeding sleeve are fixed on the first plane, described irrigation sleeve, stepper motor and jacking gear are fixed on the second plane, described liquid feeding sleeve is by sliding block vertical connecting guide rail, described irrigation sleeve connects jacking gear, described stepper motor has two, difference vertical connecting guide rail and jacking gear, the lower end of described liquid feeding sleeve is provided with the first needle tubing and the second needle tubing, the lower end of described irrigation sleeve is provided with the 3rd needle tubing and the 4th needle tubing, described the second needle tubing connects waste liquid barrel with the 3rd needle tubing by conduit, described the first needle tubing connects the damping fluid reagent bottle by conduit, described the 4th needle tubing connects the washing fluid reagent bottle by conduit.
5. a kind of liquid-based cell sample manufacturing system according to claim 4, it is characterized in that: the Length Ratio first short 0.2cm-1cm of needle tubing of described the second needle tubing, the 4th needle tubing is than the 3rd short 0.5cm-2cm of needle tubing length, the cross section of described the 3rd needle tubing and surface level have the angle of 30 °-60 °, described the 4th needle tubing lower end is crooked laterally, and angle of bend is 30 °-60 °.
6. a kind of liquid-based cell sample manufacturing system according to claim 1, it is characterized in that: described film-making plate is fixed on the film-making platform by securing member, described film-making plate is provided with a plurality of slide grooves that are arranged in parallel, and the middle part of described slide groove is provided with a pair of chute.
7. a kind of liquid-based cell sample manufacturing system according to claim 1, it is characterized in that: a plurality of control programs of storage in described microcomputer system, described control window adopts touch manner to control, multilevel menu, first order menu comprises that switch control, parameter setting, operation controls, number of samples control, programmed control and time on date controls, and Level-2 menu comprises that amount of buffer controls, takes out that specimen amount is controlled, flushing fluid is controlled, the settling time is controlled, mix number of times controls and mix liquid absorption control.
8. a liquid-based cell sample manufacturing method, is characterized in that: adopt the described liquid-based cell sample manufacturing system of any one in claim 1 to 7.
9. a kind of liquid-based cell sample manufacturing method according to claim 8, is characterized in that, comprises the following steps:
Step 1: add cell separation liquid in test tube, then sample is slowly joined in test tube along tube wall, take out supernatant after centrifugal through twice and keep sediment, then with test tube vibrations 5 to 30 seconds;
Step 2: test tube is put into the circular port of film-making platform, then transfer pipet is put into test tube, then the pre-service microslide is put into the slide groove of film-making plate, settling bin is written into the film-making plate, finally the film-making plate is fixed on the film-making platform;
Step 3: at the control window, carry out the program parameter setting, option program is controlled in first order menu, select needed program, select again parameter to control, enter Level-2 menu, amount of buffer be set, take out specimen amount, flushing fluid, settling time, mix number of times and mix liquid absorption, arrange complete after click determine then to get back to first order menu and click operation;
Step 4: under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to the test tube placement direction, cantilever moves to the test tube top by the horizontal guide rail of mechanical arm, liquid feeding sleeve down sliding on cantilever enters test tube and with transfer pipet, is locked, and the first needle tubing of liquid feeding sleeve lower end mixes by the second needle tubing after in vitro adding damping fluid; Mix rear liquid feeding sleeve upward sliding and mention the sample of specified amount by transfer pipet;
Step 5: under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to film-making plate direction, cantilever moves to the settling bin top by the horizontal guide rail of mechanical arm, and transfer pipet discharges the specified amount sample to settling bin, and microcomputer system starts countdown;
Step 6: under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to transfer pipet recovery approach direction, cantilever moves to transfer pipet recovery approach top by the horizontal guide rail of mechanical arm, by the discharge mechanism on cantilever, transfer pipet is laid;
Step 7: after the microcomputer system countdown finishes, under microcomputer system is controlled, the mechanical arm that the mobile device band is dynamically connected and cantilever move to film-making plate direction, cantilever moves to the settling bin top by the horizontal guide rail of mechanical arm, jacking gear control irrigation sleeve on cantilever moves down and enters settling bin, the 3rd needle tubing of irrigation sleeve lower end siphons away the supernatant in settling bin, then by the 4th needle tubing, injects the washing fluid of specified amount to settling bin;
Step 8: under microcomputer system was controlled, the 3rd needle tubing of irrigation sleeve lower end siphoned away washing fluid, and then the 4th needle tubing injects the washing fluid of specified amount to settling bin;
Step 9: under microcomputer system was controlled, after repeating step 8 one to three times, microcomputer system entered countdown, and after countdown finishes, the 3rd needle tubing siphons away the washing fluid in settling bin: mobile device, mechanical arm and cantilever automatically reset and stop;
Step 10: take off microslide, be placed in 95% alcohol fixing, film-making is completed.
10. a kind of liquid-based cell sample manufacturing method according to claim 9, it is characterized in that: the cell separation liquid described in step 1 is any in Percoll parting liquid, Ficoll parting liquid, damping fluid described in step 4 is any in phosphate buffer, Tris damping fluid, and step 7 is any in 95% ethanol, 100% ethanol, 50% isopropyl alcohol+50% alcohol mixture, 50% isopropyl alcohol+48% ethanol+2% carbinol mixture to the washing fluid described in step 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310368996.7A CN103398890B (en) | 2013-08-22 | 2013-08-22 | A kind of liquid-based cell sample manufacturing system and flaking method thereof |
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| CN105716918A (en) * | 2016-02-05 | 2016-06-29 | 孙胜利 | Liquid-based cell preparation device and method and film cylinder for liquid-based cell preparation |
| CN108088723A (en) * | 2018-01-30 | 2018-05-29 | 武汉呵尔医疗科技发展有限公司 | A kind of decanter type Thinprep pap test pelleter |
| CN109507004A (en) * | 2018-11-21 | 2019-03-22 | 杭州海世嘉生物科技有限公司 | A kind of natural subsidence sectioning cells dyeing work station control method and system |
| CN111487110A (en) * | 2020-04-28 | 2020-08-04 | 太原市罗塞塔石生物技术有限公司 | Gas circuit and liquid circuit integrated module for attaching glass slide |
| CN113702125A (en) * | 2021-08-12 | 2021-11-26 | 姚香怡 | Full-automatic sheet making machine and sheet making process method |
| CN114018667A (en) * | 2015-09-14 | 2022-02-08 | 国立大学法人滋贺医科大学 | Cell-retaining substrate holder for producing observation specimen, kit including same, and method for producing observation specimen |
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| CN114018667A (en) * | 2015-09-14 | 2022-02-08 | 国立大学法人滋贺医科大学 | Cell-retaining substrate holder for producing observation specimen, kit including same, and method for producing observation specimen |
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| CN109507004B (en) * | 2018-11-21 | 2021-11-02 | 杭州海世嘉生物科技有限公司 | Control method and system for natural sedimentation cell sheet-making dyeing workstation |
| CN111487110A (en) * | 2020-04-28 | 2020-08-04 | 太原市罗塞塔石生物技术有限公司 | Gas circuit and liquid circuit integrated module for attaching glass slide |
| CN111487110B (en) * | 2020-04-28 | 2023-01-31 | 太原市罗塞塔石生物技术有限公司 | Gas circuit and liquid circuit integrated module for attaching glass slide |
| CN113702125A (en) * | 2021-08-12 | 2021-11-26 | 姚香怡 | Full-automatic sheet making machine and sheet making process method |
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