CN201497725U - Controllable temperature electrolytic cell - Google Patents
Controllable temperature electrolytic cell Download PDFInfo
- Publication number
- CN201497725U CN201497725U CN2009202357747U CN200920235774U CN201497725U CN 201497725 U CN201497725 U CN 201497725U CN 2009202357747 U CN2009202357747 U CN 2009202357747U CN 200920235774 U CN200920235774 U CN 200920235774U CN 201497725 U CN201497725 U CN 201497725U
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- slide glass
- electrolytic cell
- groove
- set bolt
- solution
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Abstract
The utility model relates to a controllable temperature electrolytic cell, which comprises an solution injection and extraction hole (1), an incubation circulating water pipe winding groove (2), a reaction liquid cavity (3), an O-shaped gasket (4), a slide glass placing groove (5), a fixed screw bolt groove (6) and a fixed screw bolt (7), wherein the electrolytic cell is in a hollow structure, the solution injection and extraction hole (1) is positioned on the upper part of the electrolytic cell, fixed screw bolt groove (6) is positioned at the lower part of the electrolytic cell, the solution injection and extraction hole (1), the reaction liquid cavity (3), the slide glass placing groove (5) and the fixed screw bolt groove (6) are communicated sequentially from top to bottom, the O-shaped gasket (4) is positioned between the reaction liquid cavity (3) and the slide glass placing groove (5), the outer side of the reaction liquid cavity (3) of the electrolytic cell is the incubation circulating water pipe winding groove (2), and the fixed screw bolt (7) is screwed into the fixed screw bolt groove (6). The electrolytic cell has a fixing device of slide glass to be processed, so the area of the slide glass to be processed becomes the bottom of the reaction liquid cavity, and the utility model is favorable for the sufficient contact of the slide glass and the reaction liquid.
Description
Technical field
The utility model belongs to biological chemistry detection technique field, relates to a kind of multifunctional temperature-controllable electrolytic cell that is used for biochemical analysis.
Background technology
Electrolytic cell is a tool critical component in biology, chemistry or electrochemical analytical instrument, in slide glass finishing application facet, need slide glass and solution that fixing surface of contact is arranged, particularly the modification of biomolecule on slide glass need make the long-pending solution of microbody sufficient the contact be arranged with the slide glass surface, and modification wants to control accurately and to regulate the temperature and time of incubation, and can make things convenient for cleaning and more new soln timely, make on the slide glass and do not stay " dead liquid ".These specific requirements are that traditional electrolytic cell is difficult to satisfy simultaneously.And support that simultaneously the traditional especially electrolytic cell of multiple detection technique means is not available.And traditional electrolytic cell usually needs often to take out slide glass when carrying out the biomolecule assembling or modifying, both may cause the inactivation of biomolecule because of the change of environment, also may introduce ambient impurities, can't guarantee that multi-time modification can carry out identically with the slide glass area of multi-time modification at the same area of slide glass, this all can give and bring error in the follow-up testing result even lead to the failure.
Summary of the invention
Technical matters: at the problem that above-mentioned traditional electrolytic cell exists in biological, chemical analysis detect, the utility model provides a kind of multifunctional temperature-controllable electrolytic cell that is used for biochemical analysis, and its method for making is easy, and cost is low.Carry out biomolecule assembling on the slide glass surface or modify not needing slide glass is taken out from electrolytic cell in test process, the accurate contact area of fixed solution and slide glass can make things convenient for timely slide glass is cleaned and new soln fully more.Can make things convenient for and regulate heated culture temperature and time accurately.Support the detection method that multiple current biology/chemical detection researchs such as stripping voltammetry, chemoluminescence method, electrochemiluminescence method are commonly used simultaneously.
Technical scheme: temperature-controllable electrolytic cell of the present utility model comprises: solution filling orifice and solution are extracted the hole out, incubation circulating water pipe winding groove, and the reaction sap cavity, zero type packing ring, slide glass is put into groove, set bolt groove and set bolt; Wherein, this electrolytic cell is the structure of hollow, solution filling orifice and solution are extracted the hole out and are positioned at electrolytic cell top, the set bolt groove is positioned at the electrolytic cell bottom, solution filling orifice and solution extraction hole, reaction sap cavity, slide glass are put into groove, and set bolt groove order from top to bottom is communicated with, and zero type packing ring is put between the groove at reaction sap cavity and slide glass, the outside at this electrolytic cell reaction sap cavity is an incubation circulating water pipe winding groove, and set bolt screws in the set bolt groove.
Described slide glass is put into groove and is inserted slide glass, and the slide glass center is overlapped with zero type packing ring center substantially, set bolt is screwed in the set bolt groove hold out against slide glass.
The multifunctional temperature-controllable electrolytic cell that is used for biology, chemical analysis has slide glass stationary installation to be finished, and the area that slide glass need be modified becomes the end of reactant liquor chamber, is beneficial to slide glass and fully contacts with reactant liquor.Can be used as assembling and the modification of the protein or the DNA on slide glass surface.The electrolytic cell main body selects for use transparent material to make, and can be used as the chemiluminescent assay electrolytic cell.Two apertures are arranged at the electrolytic cell top, both can inject and the extraction hole as the solution in the modification, also can be as the electrode patchhole in the electrochemiluminescence test process, outside fluted with the fixing corresponding electrolytic cell in slide glass position, be used to twine hot-water line and carry out online temperature control incubation, can regulate and control heated culture temperature and time easily.Electrolytic cell of the present invention can be accurately the fixing contact area of slide glass and solution, help the stability and repeatability of test result.The liquid-in pipe and the Extract pipe degree of depth are annotated in self controllable insertion, make on the slide glass not stay " dead liquid ", bring into play the effect of solution and the sum of errors of minimizing mensuration rest to greatest extent and disturb, and help the modification and the test of the long-pending solution example of microbody.Advantages such as it is many that electrolytic cell of the present invention has function, easy to operate, and controllability is strong, and experimental data is reliable and stable for biology/chemical analysis experiment provides a kind of convenient and reliable instrument, are having huge application potential aspect the biochemical analysis experiment.
Beneficial effect: the utility model electrolytic cell is made main body with organic transparent material, and technology is simple, low price.Carry out that slide glass need not take out in the slide glass modification determination process; simplified experimental implementation; protected the active environment of biomolecule; avoided interference and the influence of introduced contaminants to experiment; and the slide glass area size of the each modification determination of assurance of the accurate strictness of energy is consistent; the zone is constant, has improved the accuracy and the repeatability of testing result greatly.Heated culture temperature easily and effectively and time control have improved the reliability and stability of experiment.Support the characteristic of many technical methods to establish wide application foundation in the application of biology/field of chemical detection for electrolytic cell of the present invention.
Description of drawings
Fig. 1 is the planform of an embodiment of the utility model electrolytic cell;
Fig. 2 is the sectional view of the utility model electrolytic cell;
Fig. 3 be the slide glass set bolt planform.
Have among the above figure: solution filling orifice and extraction hole 1, incubation water pipe winding groove 2, reaction sap cavity 3, zero type packing rings 4, slide glass is put into hole 5, fixed bolt hole 6, set bolt 7.
Specific implementation method
Electrolytic cell forms through machined into transparent material, profile be square or rectangle or cylindrical making comparatively convenient.Sectional view such as Fig. 2, set bolt are as shown in Figure 3.This electrolytic cell comprises: solution filling orifice and solution are extracted hole 1 out, incubation circulating water pipe winding groove 2, and reaction sap cavity 3, zero type packing rings 4, slide glass is put into groove 5, set bolt groove 6 and set bolt 7; Wherein, this electrolytic cell is the structure of hollow, solution filling orifice and solution are extracted hole 1 out and are positioned at electrolytic cell top, set bolt groove 6 is positioned at the electrolytic cell bottom, solution filling orifice and solution extraction hole 1, reaction sap cavity 3, slide glass are put into groove 5, and set bolt groove 6 order from top to bottom is communicated with, and zero type packing ring 4 is put between the groove 5 at reaction sap cavity 3 and slide glass, the outside at this electrolytic cell reaction sap cavity 3 is an incubation circulating water pipe winding groove 2, and set bolt 7 screws in the set bolt groove 6.Described slide glass is put into groove 5 and is inserted slide glass, and the slide glass center is overlapped with zero type packing ring, 4 centers substantially, set bolt 7 is screwed in the set bolt groove 6 hold out against slide glass.
The utility model electrolytic cell can be used for various biomolecules to be assembled or modifies on multiple slide glass surface, can also be used to modify chemiluminescence detection and the electrochemiluminescence detection and the stripping voltammetry detection etc. of slide glass.
The concrete using method of the utility model electrolytic cell: slide glass is inserted into the cell center position by side crossheading 5, make the slide glass center aim at the center of zero type circle 4 substantially, hold out against on zero type circle slide glass fixing with set bolt 7 (referring to Fig. 3) precession set bolt groove 6.The set bolt end is placed downwards, to contain by the solution of decorating molecule and inject reaction sap cavity 3 by a hole in 1, minimum can the infiltration fully that makes solution is exposed to the slide glass part that reaction sap cavity 3 has and do not overflow reaction sap cavity 3 at most, solution just fully contacts with slide glass, the incubation water-circulating pipe is wrapped in the temperature controlled water bath pipe winding groove 2, control water temperature and incubation time, can carry out finishing or assembling to slide glass.Incubation finishes the back waste liquid can be extracted out by another hole in 1, and the degree of depth that control extraction pipe inserts can make waste liquid extract out fully, does not stay " dead liquid ".Refill washing lotion again, extract out, repeated washing is up to reaching the required cleaning requirement of experiment.Repeatedly assemble or modify as need, need not take out slide glass, repeat aforementioned operation and get final product.
After assembling or modification are finished, electrolytic cell of the present invention is also supported multiple detection technique means, do not taking out under the preceding topic of slide glass, as slide glass assembling or modification is chemiluminescent material, in electrolytic cell, inject the chemiluminescence inducing agent by hole 1, can put into optical detection apparatus to electrolytic cell integral body and detect chemiluminescence signal.If assembling or modification is the electrochemiluminescence material on the slide glass, in electrolytic cell, inject the electrochemiluminescence inducing agent by hole 1, in two holes of 1, insert contrast electrode respectively then and to electrode, with the slide glass is working electrode, can put into optical detection apparatus to electrolytic cell integral body and detect the electrochemiluminescence signal.If be modified with the micro nano structure of metallic compound on the slide glass, can in electrolytic cell, add acid solution by hole 1, be the corresponding metal ion with metal compound solution, by hole 1 solution to be extracted out then, the washing lotion of reinjecting is extracted washing lotion out again.Can merge and to carry out follow-up metallic ion and measure being dissolved with metal ion solution and washing lotion, as stripping voltammetry etc.
Claims (2)
1. a temperature-controllable electrolytic cell is characterized in that this electrolytic cell comprises: solution filling orifice and solution extraction hole (1), incubation circulating water pipe winding groove (2), reaction sap cavity (3), O type packing ring (4), slide glass are put into groove (5), set bolt groove (6) and set bolt (7); Wherein, this electrolytic cell is the structure of hollow, solution filling orifice and solution are extracted hole (1) out and are positioned at electrolytic cell top, set bolt groove (6) is positioned at the electrolytic cell bottom, solution filling orifice and solution extraction hole (1), reaction sap cavity (3), slide glass are put into groove (5), set bolt groove (6) order from top to bottom is communicated with, O type packing ring (4) is positioned at reaction sap cavity (3) and slide glass is put between the groove (5), the outside at this electrolytic cell reaction sap cavity (3) is an incubation circulating water pipe winding groove (2), and set bolt (7) screws in the set bolt groove (6).
2. temperature-controllable electrolytic cell according to claim 1 is characterized in that described slide glass puts into groove (5) and insert slide glass, and the slide glass center is overlapped with O type packing ring (4) center substantially, set bolt (7) is screwed in the set bolt groove (6) hold out against slide glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202357747U CN201497725U (en) | 2009-09-23 | 2009-09-23 | Controllable temperature electrolytic cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202357747U CN201497725U (en) | 2009-09-23 | 2009-09-23 | Controllable temperature electrolytic cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201497725U true CN201497725U (en) | 2010-06-02 |
Family
ID=42440929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009202357747U Expired - Lifetime CN201497725U (en) | 2009-09-23 | 2009-09-23 | Controllable temperature electrolytic cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201497725U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101672816B (en) * | 2009-09-23 | 2012-06-27 | 东南大学 | Multifunctional temperature-controllable electrolytic cell for biological and chemical analysis |
| CN103995032A (en) * | 2014-04-21 | 2014-08-20 | 南通大学 | Photoelectrochemical electrolytic cell structure using leaf spring as lead wire |
| CN105675680A (en) * | 2014-04-21 | 2016-06-15 | 南通大学 | Double-chamber photoelectrochemistry electrolytic tank |
-
2009
- 2009-09-23 CN CN2009202357747U patent/CN201497725U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101672816B (en) * | 2009-09-23 | 2012-06-27 | 东南大学 | Multifunctional temperature-controllable electrolytic cell for biological and chemical analysis |
| CN103995032A (en) * | 2014-04-21 | 2014-08-20 | 南通大学 | Photoelectrochemical electrolytic cell structure using leaf spring as lead wire |
| CN103995032B (en) * | 2014-04-21 | 2016-05-04 | 南通大学 | A kind of with spring leaf work lead-in wire Optical Electro-Chemistry electrolyser construction |
| CN105675680A (en) * | 2014-04-21 | 2016-06-15 | 南通大学 | Double-chamber photoelectrochemistry electrolytic tank |
| CN105675680B (en) * | 2014-04-21 | 2019-07-19 | 南通大学 | A kind of dual chamber optical electro-chemistry electrolytic cell |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20100602 Effective date of abandoning: 20090923 |
|
| RGAV | Abandon patent right to avoid regrant |