CN101672816B - Multifunctional temperature-controllable electrolytic cell for biological and chemical analysis - Google Patents

Multifunctional temperature-controllable electrolytic cell for biological and chemical analysis Download PDF

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Publication number
CN101672816B
CN101672816B CN2009100353784A CN200910035378A CN101672816B CN 101672816 B CN101672816 B CN 101672816B CN 2009100353784 A CN2009100353784 A CN 2009100353784A CN 200910035378 A CN200910035378 A CN 200910035378A CN 101672816 B CN101672816 B CN 101672816B
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China
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slide glass
electrolytic cell
groove
bolt
solution
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CN2009100353784A
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CN101672816A (en
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徐春祥
谷保祥
刘松琴
王明亮
朱光平
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HAIAN TONGYUAN YARN CO., LTD.
Southeast University
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Southeast University
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Abstract

The invention relates to a multifunctional temperature-controllable electrolytic cell for biological and chemical analysis, which is provided with a fixing device of a slide glass to be decorated, and the fixing device ensures that an area of the slide glass, which is to be decorated, becomes the bottom of a reaction liquid chamber so as to be beneficial to the sufficient contact between the slide glass and the reaction liquid. The electrolytic cell comprises a solution injection and pump-out hole (1), an incubation circulating pipe winding groove (2), a reaction liquid cavity (3), an O-shaped ring (4), a slide glass put-in groove (5), a fixing bolt groove (6) and a fixing bolt (7), wherein the electrolytic cell has a hollow structure; the solution injection and pump-out hole (1) is arranged on the upper part of the electrolytic cell, and the fixing bolt groove (6) is arranged on the lower part of the electrolytic cell; the solution injection and pump-out hole (1), the reaction liquid cavity (3), the slide glass put-in groove (5) and the fixing bolt groove (6) are sequentially communicated from top down; the O-shaped ring (4) is arranged between the reaction liquid cavity (3) and the slide glass put-in groove (5); the incubation circulating pipe winding groove (2) is arranged outside the reaction liquid cavity (3); and the fixing bolt (7) is screwed into the fixing bolt groove (6).

Description

The multifunctional temperature-controllable electrolytic cell that is used for biology, chemical analysis
Technical field
The invention belongs to biological chemistry detection technique field, relate to a kind of multifunctional temperature-controllable electrolytic cell that is used for biochemical analysis.
Background technology
Electrolytic cell is a tool critical component in biology, chemistry or electrochemical analytical instrument; In slide glass finishing application facet; Need slide glass and solution to have the modification on slide glass of fixing surface of contact, particularly biomolecule need make the long-pending solution of microbody sufficient the contact arranged, and modification want to control accurately and to regulate the temperature and time of incubation with the slide glass surface; And can make things convenient for cleaning and more new soln timely, make on the slide glass and do not stay " dead liquid ".These specific requirements are that traditional electrolytic cell is difficult to satisfy simultaneously.And support that simultaneously the traditional especially electrolytic cell of multiple detection technique means is not available.And traditional electrolytic cell usually needs often to take out slide glass when carrying out the biomolecule assembling or modifying; Both possibly cause the inactivation of biomolecule because of the change of environment; Also possibly introduce ambient impurities; Can't guarantee that multi-time modification can carry out identically with the slide glass area of multi-time modification at the same area of slide glass, this all can give and bring error in the follow-up testing result even lead to the failure.
Summary of the invention
Technical matters: the problem to above-mentioned traditional electrolytic cell exists in biological, chemical analysis detect, the invention provides a kind of multifunctional temperature-controllable electrolytic cell that is used for biochemical analysis, its method for making is easy, and cost is low.Carry out biomolecule assembling on the slide glass surface or modify and in test process, need from electrolytic cell, not take out slide glass, the accurate contact area of fixed solution and slide glass can make things convenient for and timely slide glass cleaned and new soln fully more.Can make things convenient for and regulate heated culture temperature and time accurately.Support the detection method that multiple current biology/chemical detection researchs such as stripping voltammetry, chemoluminescence method, electrochemiluminescence method are commonly used simultaneously.
Technical scheme: the multifunctional temperature-controllable electrolytic cell that the present invention is used for biology, chemical analysis comprises: solution filling orifice and solution are extracted the hole out, incubation circulating water pipe winding groove, and the reaction sap cavity, zero type packing ring, slide glass is put into groove, set bolt groove and set bolt; Wherein, this electrolytic cell is the structure of hollow, and solution filling orifice and solution are extracted the hole out and is positioned at electrolytic cell top; The set bolt groove is positioned at the electrolytic cell bottom; Solution filling orifice and solution extraction hole, reaction sap cavity, slide glass are put into groove, and set bolt groove order from top to bottom is communicated with, and zero type packing ring is put between the groove at reaction sap cavity and slide glass; The outside at this electrolytic cell reaction sap cavity is an incubation circulating water pipe winding groove, and set bolt screws in the set bolt groove.
Described slide glass is put into groove and is inserted slide glass, and the slide glass center is overlapped with zero type packing ring center basically, screws in set bolt in the set bolt groove and holds out against slide glass.
The multifunctional temperature-controllable electrolytic cell that is used for biology, chemical analysis has slide glass stationary installation to be finished, and the area that slide glass need be modified becomes the end of reactant liquor chamber, is beneficial to slide glass and fully contacts with reactant liquor.Can be used as the protein on slide glass surface or assembling and the modification of DNA.The electrolytic cell main body selects for use transparent material to process, and can be used as the chemiluminescent assay electrolytic cell.Two apertures are arranged at the electrolytic cell top; Both can inject and the extraction hole as the solution in the modification; Also can be as the electrode patchhole in the electrochemiluminescence test process; Outside fluted with the fixing corresponding electrolytic cell in slide glass position is used to twine hot-water line and carries out online temperature control incubation, can regulate and control heated culture temperature and time easily.Electrolytic cell of the present invention can be accurately the fixing contact area of slide glass and solution, help the stability and repeatability of test result.The liquid-in pipe and the Extract pipe degree of depth are annotated in self controllable insertion, make on the slide glass not stay " dead liquid ", bring into play the effect of solution and the sum of errors of minimizing mensuration rest to greatest extent and disturb, and help the modification and the test of the long-pending solution example of microbody.Advantages such as it is many that electrolytic cell of the present invention has function, easy to operate, and controllability is strong, and experimental data is reliable and stable for biology/chemical analysis experiment provides a kind of convenient and reliable instrument, are having huge application potential aspect the biochemical analysis experiment.
Beneficial effect: electrolytic cell of the present invention is made main body with organic transparent material, and technology is simple, low price.Carry out that slide glass need not take out in the slide glass modification determination process; Simplified experimental implementation; Protected the active environment of biomolecule, avoided interference and the influence of introduced contaminants, and the slide glass area size of the each modification determination of assurance of the accurate strictness of ability is consistent experiment; The zone is constant, has improved the accuracy and the repeatability of testing result greatly.Heated culture temperature easily and effectively and time control have improved the reliability and stability of experiment.Support the characteristic of many technical methods to establish wide application foundation in the application of biology/field of chemical detection for electrolytic cell of the present invention.
Description of drawings
Fig. 1 is the planform of an embodiment of electrolytic cell of the present invention;
Fig. 2 is the sectional view of electrolytic cell of the present invention;
Fig. 3 be the slide glass set bolt planform.
Have among the above figure: solution filling orifice and extraction hole 1, incubation water pipe winding groove 2, reaction sap cavity 3, zero type packing rings 4, slide glass is put into hole 5, fixed bolt hole 6, set bolt 7.
The practical implementation method
Electrolytic cell forms through machined into transparent material, profile be square or rectangle or cylindrical making comparatively convenient.Sectional view such as Fig. 2, set bolt is as shown in Figure 3.This electrolytic cell comprises: solution filling orifice and solution are extracted hole 1 out, incubation circulating water pipe winding groove 2, and reaction sap cavity 3, zero type packing rings 4, slide glass is put into groove 5, set bolt groove 6 and set bolt 7; Wherein, this electrolytic cell is the structure of hollow, and solution filling orifice and solution are extracted hole 1 out and is positioned at electrolytic cell top; Set bolt groove 6 is positioned at the electrolytic cell bottom; Solution filling orifice and solution extraction hole 1, reaction sap cavity 3, slide glass are put into groove 5, and set bolt groove 6 order from top to bottom is communicated with, and zero type packing ring 4 is put between the groove 5 at reaction sap cavity 3 and slide glass; The outside at this electrolytic cell reaction sap cavity 3 is an incubation circulating water pipe winding groove 2, and set bolt 7 screws in the set bolt groove 6.Described slide glass is put into groove 5 and is inserted slide glass, and the slide glass center is overlapped with zero type packing ring, 4 centers basically, screws in set bolt 7 in the set bolt groove 6 and holds out against slide glass.
Electrolytic cell of the present invention can be used for various biomolecules to be assembled or modifies on multiple slide glass surface, can also be used to modify chemiluminescence detection and the electrochemiluminescence detection and the stripping voltammetry detection etc. of slide glass.
The concrete method of application of electrolytic cell of the present invention: slide glass is inserted into the cell center position by side crossheading 5, makes the slide glass center aim at the center of zero type circle 4 basically, hold out against on zero type circle slide glass fixing with set bolt 7 (referring to Fig. 3) precession set bolt groove 6.The set bolt end is to held; To contain by the solution of decorating molecule and inject reaction sap cavity 3 through a hole in 1; Minimum can the infiltration fully that makes solution is exposed to the slide glass part that reaction sap cavity 3 has and do not overflow reaction sap cavity 3 at most, and solution just fully contacts with slide glass, and the incubation water-circulating pipe is wrapped in the temperature controlled water bath pipe winding groove 2; Control water temperature and incubation time, can carry out finishing or assembling to slide glass.Incubation finishes the back waste liquid can be extracted out by another hole in 1, and the degree of depth that control extraction pipe inserts can make waste liquid extract out fully, does not stay " dead liquid ".Refill washing lotion again, extract out, repeated washing is up to reaching the required cleaning requirement of experiment.Repeatedly assemble or modify like need, need not take out slide glass, repeat aforementioned operation and get final product.
After assembling or modification are accomplished; Electrolytic cell of the present invention is also supported multiple detection technique means; Do not taking out under the preceding topic of slide glass; Like slide glass assembling or what modify is chemiluminescent material, in electrolytic cell, inject the chemiluminescence inducing agent through hole 1, can put into optical detection apparatus to electrolytic cell integral body and detect chemiluminescence signal.If assembling or modification is the electrochemiluminescence material on the slide glass; In electrolytic cell, inject the electrochemiluminescence inducing agent through hole 1; In two holes of 1, insert contrast electrode respectively then and to electrode; With the slide glass is working electrode, can put into optical detection apparatus to electrolytic cell integral body and detect the electrochemiluminescence signal.If be modified with the micro nano structure of metallic compound on the slide glass, can in electrolytic cell, add acid solution through hole 1, be the corresponding metal ion with metal compound solution, to extract solution out through hole 1 then, the washing lotion of reinjecting is extracted washing lotion out again.Can merge and to carry out follow-up metallic ion and measure being dissolved with metal ion solution and washing lotion, like stripping voltammetry etc.

Claims (2)

1. multifunctional temperature-controllable electrolytic cell that is used for biology, chemical analysis; It is characterized in that this electrolytic cell comprises: solution filling orifice and solution are extracted hole (1) out; Incubation circulating water pipe winding groove (2), reaction sap cavity (3), O type packing ring (4); Slide glass is put into groove (5), set bolt groove (6) and set bolt (7); Wherein, This electrolytic cell is the structure of hollow; Solution filling orifice and solution are extracted hole (1) out and are positioned at electrolytic cell top, and set bolt groove (6) is positioned at the electrolytic cell bottom, and solution filling orifice and solution extraction hole (1), reaction sap cavity (3), slide glass are put into groove (5); Set bolt groove (6) order from top to bottom is communicated with; O type packing ring (4) is positioned at reaction sap cavity (3) and slide glass is put between the groove (5), is incubation circulating water pipe winding groove (2) in the outside of this electrolytic cell reaction sap cavity (3), and set bolt (7) screws in the set bolt groove (6).
2. the multifunctional temperature-controllable electrolytic cell that is used for biology, chemical analysis according to claim 1; It is characterized in that described slide glass puts into groove (5) and insert slide glass; The slide glass center is overlapped with O type packing ring (4) center basically, screw in set bolt (7) in the set bolt groove (6) and hold out against slide glass.
CN2009100353784A 2009-09-23 2009-09-23 Multifunctional temperature-controllable electrolytic cell for biological and chemical analysis Active CN101672816B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108896630B (en) * 2018-06-11 2020-05-12 杨韫瑜 Multifunctional temperature-controllable electrolytic cell for biological and chemical analysis
CN110146561A (en) * 2019-05-16 2019-08-20 大连诚泽检测有限公司 A kind of 15 microlitres of negotiable electrolytic cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1068661A (en) * 1962-10-13 1967-05-10 Lucia Eschweiler Electrical gas analysis equipment
EP0875755A1 (en) * 1997-04-29 1998-11-04 Eastman Kodak Company Device for electrochemical detection or measurement
CN201007715Y (en) * 2007-02-15 2008-01-16 厦门大学 Flow system electrochemiluminescence electrolyser for indium-stannum oxide glass electrode
CN201497725U (en) * 2009-09-23 2010-06-02 东南大学 Controllable temperature electrolytic cell

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1068661A (en) * 1962-10-13 1967-05-10 Lucia Eschweiler Electrical gas analysis equipment
EP0875755A1 (en) * 1997-04-29 1998-11-04 Eastman Kodak Company Device for electrochemical detection or measurement
CN201007715Y (en) * 2007-02-15 2008-01-16 厦门大学 Flow system electrochemiluminescence electrolyser for indium-stannum oxide glass electrode
CN201497725U (en) * 2009-09-23 2010-06-02 东南大学 Controllable temperature electrolytic cell

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JP昭58-55763A 1983.04.02

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