CN104383568A - Simple soil sterilizing device and sterilizing method by using same - Google Patents
Simple soil sterilizing device and sterilizing method by using same Download PDFInfo
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- CN104383568A CN104383568A CN201410629529.XA CN201410629529A CN104383568A CN 104383568 A CN104383568 A CN 104383568A CN 201410629529 A CN201410629529 A CN 201410629529A CN 104383568 A CN104383568 A CN 104383568A
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Abstract
The invention discloses a simple soil sterilizing device and a sterilizing method by using the same. A florence flask is arranged in a constant-temperature water bath pot, and a first beaker is arranged in the florence flask; a fourth valve and a first air filter are sequentially arranged at the right upper part of the florence flask; a feed pipe is arranged at the left upper part of the florence flask; a triangular conical bottle cap is arranged at the top end of the florence flask; the triangular conical bottle cap, a sixth valve, a second air filter, a triangular conical bottle cap, second filter paper, a polyethylene pipe, first filter paper, a triangular conical bottle cap, a third air filter, an air pump, a third valve and a middle large air inlet of the florence flask are sequentially connected; a fifth valve and a fourth air filter are connected between the second air filter and the sixth valve by virtue of a tee pipe; a second valve is connected between the air pump and the third valve by virtue of a tee pipe; and a second beaker is arranged below the second valve. The device can be used for relieving the influence of the sterilizing process on the physicochemical properties of soil, improving the efficiency of soil sterilization and reducing harm to environment and testing personnel.
Description
Technical field
The present invention relates to bactericidal unit and method thereof, particularly relate to a kind of Simple soil bactericidal unit and sterilizing methods thereof.
Background technology
Soil microorganism refers to the general name of the antibacterial lived in soil, fungus, actinomycetes, algae, its kind and quantity with soil-forming conditions and soil depth thereof difference and change.The processes such as they carry out being oxidized in soil, nitrated, ammonification, fixed nitrogen, sulfuration, promote the decomposition of the soil organism and the conversion of nutrient.Nearly all soil course is relevant with soil microorganism all directly or indirectly.The complexity of soil microorganism kind, quantity and function, on research soil in predetermined substance especially organic substance conversion and on the effect of plant growing, there is larger impact.In the impact test of the specific organic pollution of research on plant growing, majority of organic pollutants is decomposed by the microorganisms or is converted into other organic pollutions, thus is difficult to the murder by poisoning degree of accurate evaluation certain concentration organic pollution.In organic nutrition of plant development test, the organic substance such as aminoacid, glucose, nucleic acid that soil itself and external source are added is decomposed by the microorganisms utilization, is converted into inorganic nitrogen, thus zooms in or out organic substance of knowing clearly and contribute the nutrition of plant growing.Adopt a kind of simple, safety and Soil structure and the less soil disinfection method of organic substance impact become to the key of above-mentioned test.
Current soil disinfection method, is mainly used in the upper field soil of production and disinfects.Chemical method is one of soil disinfection method comparatively commonly used at present.Conventional chemical agent is formaldehyde, 1, chlorallylene and chloropicrin and methyl bromide, sulphuric acid fluorine, ammonia, lime nitrogen etc., also has formalin to mix soil or Cosan is fumigated, the methods such as peracetic acid layering sprinkling.These medicaments can remain in soil, cause certain influence to follow-up test, and some poisonous and harmful drug volatilization can damage testing crew.Be in the patent of invention of 200710015138.9, disclose a kind of soil sterilization and disinsection car at the peripheral coated electric heating device of rotary blade in the patent No., be in the patent of invention of 201110279644.5 in the patent No., toasted by the soil of flame-thrower nozzle to spin-up.These inventions have good effect to harmful microorganism in minimizing field soil, but it just kills part microorganism, cannot reach the object of thorough sterilizing.And adopt the way of high-temperature baking, also can produce larger impact to organic matter content in soil.
In laboratory, the method for soil disinfection mainly comprises high temperature sterilization, γ ray sterilization method, chemical fumigation method.High temperature sterilization is the microorganism killed by high temperature in soil, thus reaches aseptic condition; γ ray sterilization method is the feature high according to energy of γ ray, penetration power is strong, adopts Co80 to radiate gamma-rays, the C-C key of various active substance such as between DNA in cell is ruptured, thus reaches the object of killing soil microorganism.At 120 DEG C, sterilizing 30min to 4h is not etc. usually for high temperature sterilize.Darbar SR (2007) research shows that high temperature sterilization can affect the physicochemical property (pH value, cations, aggregate etc.) of soil.Because high temperature sterilization changes physicochemical property and the biological property of soil, tightness and the result of the test of test so certainly will be affected.Although the impact of γ ray sterilization method on soil is less than high temperature sterilization, due to safety and the high cost of operating condition, be difficult to universal use.The operation of chemical fumigation method is simpler than above two kinds of methods, and sterilization effect is also ideal, but it can cause the pollution of surrounding, can cause certain injury to testing crew.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of Simple soil bactericidal unit and sterilizing methods thereof are provided.
Simple soil bactericidal unit comprises large-scale boiling flask, first beaker, sample feeding pipe, first valve, second beaker, second valve, 3rd valve, first air filter, 4th valve, air pump, second air filter, polyethylene tube, triangular pyramidal bottle cap, 3rd air filter, first filter paper, triangular pyramidal bottle cap, second filter paper, 5th valve the 4th air filter, 5th valve, the triangular pyramidal bottle cap of upper end open, 6th valve, ferrum silk, thermostat water bath, potassium permanganate, pedotheque, ammonia, be provided with large-scale boiling flask in thermostat water bath, be provided with the first beaker in large-scale boiling flask, place potassium permanganate in the first beaker, the first beaker is fixed with iron wire, and the gas feed of large-scale boiling flask upper right quarter is provided with the 4th valve in turn, first air filter, the liquid-inlet of large-scale boiling flask upper left quarter is provided with sample feeding pipe, and sample feeding pipe one end is above the first beaker, and the sample feeding pipe other end is provided with the first valve, and large-scale boiling flask top is provided with triangular pyramidal bottle cap, triangular pyramidal bottle cap, 6th valve, second air filter, triangular pyramidal bottle cap, second filter paper, polyethylene tube, first filter paper, triangular pyramidal bottle cap, 3rd air filter, air pump, 3rd valve, the import of large-scale boiling flask central gas is connected in turn, places between pedotheque second air filter and the 6th valve by tee T and the 5th valve in polyethylene tube, 4th air filter is connected, and is connected, is provided with the second beaker below the second valve, hold ammonia in the second beaker between air pump with the 3rd valve by tee T with the second valve.
The step of Simple soil sterilizing methods is as follows:
1) by add in beaker 5 ?10g potassium permanganate, put into boiling flask; To complete by all sections fit except splendid attire soil sample parts, open thermostat water bath, control temperature be constant at 60 ?80 DEG C;
2) the second filter paper is positioned in triangular pyramidal bottle cap, triangular pyramidal bottle cap is tightened against in polyethylene tube, by cross 1 ?2mm sieve soil sample 30 ?50g be loaded in the polyethylene tube tightening below lid, afterwards the top triangular pyramidal bottle cap having added the first filter paper is tightened, be connected with the miscellaneous part assembled;
3) keep the second valve, the 4th valve, the 5th valve to be in closed condition, the first valve, the 3rd valve, the 6th valve stay open state; By sample feeding pipe add formalin 20 ?40ml, close the first valve rapidly, 3 ?after 5 minutes, be full of formaldehyde gas in large-scale boiling flask;
4) open air pump, formaldehyde gas circulate 20 ?after 30 minutes, open the second valve, the 5th valve, close the 3rd valve, the 6th valve, bleed 20 ?30 minutes, to pump the formaldehyde existed in soil;
5) close air pump, two ends are taken off together with the splendid attire soil parts polyethylene tube of the second air filter, the 3rd air filter, triangular pyramidal bottle cap, the first filter paper, triangular pyramidal bottle cap, the second filter paper, takes on aseptic operating platform for subsequent use;
6) other soil subject to sterilization is loaded in polyethylene tube, repeat step 2) ?step 5), after all confession examination soil disinfections complete, after all samples sterilizing completes, close the first valve, the 5th valve, the 3rd valve, open the second valve, the 4th valve, the 6th valve, bleed 30 ?40 minutes, formaldehyde gas imports the second beaker 5 that ammonia is housed, formaldehyde sucking filtration in large-scale boiling flask is clean, close thermostat water bath, after temperature reduces, first beaker is taken out, cleans the rear remaining residue of reaction.
The present invention is reacted by formaldehyde and potassium permanganate, a large amount of formaldehyde gases will be discharged in short time, formaldehyde gas after thermostat water bath heating imports in soil endlessly, Soil Microorganism can be killed, after sterilizing, pass into a large amount of filtrated airs through air filter filtration and just can reduce formaldehyde remaining in soil.The Simple soil bactericidal unit utilizing the present invention to design, can alleviate the impact of sterilization process on soil physico-chemical property, improves the efficiency of soil disinfection, reduces the injury to environment and testing crew.
Accompanying drawing explanation
Accompanying drawing is Simple soil bactericidal unit structural representation.
Detailed description of the invention
As shown in drawings, Simple soil bactericidal unit comprises large-scale boiling flask 1, first beaker 2, sample feeding pipe 3, first valve 4, second beaker 5, second valve 6, 3rd valve 7, first air filter 8, 4th valve 9, air pump 10, second air filter 11, polyethylene tube 12, triangular pyramidal bottle cap 13, 3rd air filter 14, first filter paper 15, triangular pyramidal bottle cap 16, second filter paper 17, 4th air filter 18, 5th valve 19, the triangular pyramidal bottle cap 20 of upper end open, 6th valve 21, iron wire 22, thermostat water bath 23, potassium permanganate 24, pedotheque 25, ammonia 26,
Be provided with large-scale boiling flask 1 in thermostat water bath 23, be provided with in large-scale boiling flask 1 in first beaker 2, first beaker 2 and placement potassium permanganate 24, first beaker 2 is fixed with iron wire 22, the gas feed of large-scale boiling flask 1 upper right quarter is provided with the 4th valve 9 in turn, first air filter 8, the liquid-inlet of large-scale boiling flask 1 upper left quarter is provided with sample feeding pipe 3, and sample feeding pipe 3 one end is above the first beaker 2, and sample feeding pipe 3 other end is provided with the first valve 4, large-scale boiling flask 1 top is provided with triangular pyramidal bottle cap 20, triangular pyramidal bottle cap 20, 6th valve 21, second air filter 11, triangular pyramidal bottle cap 16, second filter paper 17, polyethylene tube 12, first filter paper 15, triangular pyramidal bottle cap 13, 3rd air filter 14, air pump 10, 3rd valve 7, the import of large-scale boiling flask 1 central gas is connected in turn, places between pedotheque 25, second air filter 11 and the 6th valve 21 by tee T and the 5th valve 19 in polyethylene tube 12, 4th air filter 18 is connected, and is connected, is provided with in the second beaker 5, second beaker 5 and holds ammonia below the second valve 6 between air pump 10 with the 3rd valve 7 by tee T with the second valve 6.
The step of Simple soil sterilizing methods is as follows:
1) by add in beaker 25 ?10g potassium permanganate 24, put into boiling flask 1; To complete by all sections fit except splendid attire soil sample parts, open thermostat water bath 23, control temperature be constant at 60 ?80 DEG C;
2) the second filter paper 17 is positioned in triangular pyramidal bottle cap 16, triangular pyramidal bottle cap 16 is tightened against in polyethylene tube 12, by cross 1 ?2mm sieve pedotheque 30 ?50g be loaded in the polyethylene tube 12 tightening below lid, afterwards the top triangular pyramidal bottle cap 13 having added the first filter paper 15 is tightened, be connected with the miscellaneous part assembled;
3) keep the second valve 6, the 4th valve 9, the 5th valve 19 to be in closed condition, the first valve 4, the 3rd valve 7, the 6th valve 21 stay open state; By sample feeding pipe 3 add formalin 20 ?40ml, close rapidly the first valve 4,3 ?after 5 minutes, be full of formaldehyde gas in large-scale boiling flask 1;
4) open air pump 10, formaldehyde gas circulate 20 ?after 30 minutes, open valve second valve the 6, the 5th valve 19, close the 3rd valve 7, the 6th valve 21, bleed 20 ?30 minutes, to pump the formaldehyde existed in soil;
5) air pump 10 is closed, two ends are taken off together with the splendid attire soil parts polyethylene tube 12 of the second air filter 11, the 3rd air filter 14, triangular pyramidal bottle cap 13, first filter paper 15, triangular pyramidal bottle cap 16, second filter paper 17, takes on aseptic operating platform for subsequent use;
6) other soil subject to sterilization is loaded in polyethylene tube, repeat step 2) ?step 5), after all confession examination soil disinfections complete, after all samples sterilizing completes, close the first valve 4, the 5th valve 19, the 3rd valve 7, open the second valve 6, the 4th valve 9, the 6th valve 21, bleed 30 ?40 minutes, formaldehyde gas imports the second beaker 5 that ammonia 26 is housed, after formaldehyde sucking filtration in large-scale boiling flask 1 is clean, close thermostat water bath 23, after temperature reduces, first beaker 2 is taken out, cleans the rear remaining residue of reaction.
Embodiment 1:
1) add 5g potassium permanganate 24 by beaker 2, put into boiling flask 1; To complete by all sections fit except splendid attire soil sample parts, open thermostat water bath 23, control temperature is constant at 60 DEG C;
2) the second filter paper 17 is positioned in triangular pyramidal bottle cap 16, triangular pyramidal bottle cap 16 is tightened against in polyethylene tube 12, by be collected in West Zone of Zijingang Campus of Zhejiang University, Xihu District, Zhejiang Province fresh soil samples, ventilate in the cool and dry, cross 1mm nylon mesh.The pedotheque 30g taking 1mm sieve is loaded in the polyethylene tube 12 tightening below lid, is tightened by the top triangular pyramidal bottle cap 13 having added the first filter paper 15 afterwards, is connected with the miscellaneous part assembled;
3) keep the second valve 6, the 4th valve 9, the 5th valve 19 to be in closed condition, the first valve 4, the 3rd valve 7, the 6th valve 21 stay open state; Add formalin 20ml by sample feeding pipe 3, close rapidly the first valve after 4,5 minutes, in large-scale boiling flask 1, be full of formaldehyde gas;
4) open air pump 10, after formaldehyde gas circulates 20 minutes, open the second valve 6, the 5th valve 19, close the 3rd valve 7, the 6th valve 21, bleed 20 minutes, to pump the formaldehyde existed in soil;
5) air pump 10 is closed, two ends are taken off together with the splendid attire soil parts polyethylene tube 12 of the second air filter 11, the 3rd air filter 14, triangular pyramidal bottle cap 13, first filter paper 15, triangular pyramidal bottle cap 16, second filter paper 17, takes on aseptic operating platform for subsequent use;
6) other soil subject to sterilization is loaded in polyethylene tube, repeat step 2) ?step 5), after all confession examination soil disinfections complete, after all samples sterilizing completes, close the first valve 4, the 5th valve 19, the 3rd valve 7, open the second valve 6, the 4th valve 9, the 6th valve 21, bleed 30 minutes, formaldehyde gas imports and is equipped with in the second beaker 5 of ammonia 26, formaldehyde sucking filtration in large-scale boiling flask 1 is clean, close thermostat water bath 23, after temperature reduces, first beaker 2 is taken out, cleans the rear remaining residue of reaction.
Aseptic operating platform carries out the test of inspection bacterium, adopts beef extract-peptone to detect whether sterilizing is complete.Concrete operations are for getting 10g beef extract-peptone in 1000ml flask, 121 DEG C of sterilizings 30 minutes in high-pressure sterilizing pot, when temperature is down to about 80 DEG C, on aseptic operating platform, about 15ml is in the culture dish of 10cm in diameter, after complete cooled and solidified, adopts and burns sterilizing tweezers gripping trace soil in culture dish, cultivate 10 days at 25 DEG C, observe colony growth situation.
Can find out according to inspection bacterium result, the completely air-dry pedotheque crossing 1mm sieve, fumigate through 20 minutes formaldehyde gases, sterile soil sample accounts for 87.5% of total test sample completely, show that the method effectively can kill Soil Microorganism, guarantee test is to aseptic requirement.
| Pedotheque | Whether contain bacterium |
| 1 | No |
| 2 | No |
| 3 | No |
| 4 | No |
| 5 | Be |
| 6 | No |
| 7 | No |
| 8 | No |
Embodiment 2:
1) add 10g potassium permanganate 24 by beaker 2, put into boiling flask 1; To complete by all sections fit except splendid attire soil sample parts, open thermostat water bath 23, control temperature is constant at 80 DEG C;
2) the second filter paper 17 is positioned in triangular pyramidal bottle cap 16, triangular pyramidal bottle cap 16 is tightened against in polyethylene tube 12, by be collected in West Zone of Zijingang Campus of Zhejiang University, Xihu District, Zhejiang Province fresh soil samples, ventilate in the cool and dry, cross 1mm nylon mesh.The pedotheque 50g taking 1mm sieve is loaded in the polyethylene tube 12 tightening below lid, is tightened by the top triangular pyramidal bottle cap 13 having added the first filter paper 15 afterwards, is connected with the miscellaneous part assembled;
3) keep the second valve 6, the 4th valve 9, the 5th valve 19 to be in closed condition, the first valve 4, the 3rd valve 7, the 6th valve 21 stay open state; Add formalin 40ml by sample feeding pipe 3, close rapidly the first valve after 4,5 minutes, in large-scale boiling flask 1, be full of formaldehyde gas;
4) open air pump 10, after formaldehyde gas circulates 30 minutes, open the second valve 6, the 5th valve 19, close the 3rd valve 7, the 6th valve 21, bleed 30 minutes, to pump the formaldehyde existed in soil;
5) air pump 10 is closed, two ends are taken off together with the splendid attire soil parts polyethylene tube 12 of the second air filter 11, the 3rd air filter 14, triangular pyramidal bottle cap 13, first filter paper 15, triangular pyramidal bottle cap 16, second filter paper 17, takes on aseptic operating platform for subsequent use;
6) other soil subject to sterilization is loaded in polyethylene tube, repeat step 2) ?step 5), after all confession examination soil disinfections complete, after all samples sterilizing completes, close the first valve 4, the 5th valve 19, the 3rd valve 7, open the second valve 6, the 4th valve 9, the 6th valve 21, bleed 40 minutes, formaldehyde gas imports and is equipped with in the second beaker 5 of ammonia 26, formaldehyde sucking filtration in large-scale boiling flask 1 is clean, close thermostat water bath 23, after temperature reduces, first beaker 2 is taken out, cleans the rear remaining residue of reaction.
Aseptic operating platform carries out the test of inspection bacterium, adopts beef extract-peptone to detect whether sterilizing is complete.Concrete operations are for getting 10g beef extract-peptone in 1000ml flask, 121 DEG C of sterilizings 30 minutes in high-pressure sterilizing pot, when temperature is down to about 80 DEG C, on aseptic operating platform, about 15ml is in the culture dish of 10cm in diameter, after complete cooled and solidified, adopts and burns sterilizing tweezers gripping trace soil in culture dish, cultivate 10 days at 25 DEG C, observe colony growth situation.
Can find out according to inspection bacterium result, the completely air-dry pedotheque crossing 1mm sieve, fumigate through 30 minutes formaldehyde gases, sterile soil sample accounts for 100% of total test sample completely, show that the method effectively can kill Soil Microorganism, and sterilization effect with formaldehyde gas concentration and temperature higher, sterilization time increase and improve, follow-up test can be met to aseptic requirement.
| Pedotheque | Whether contain bacterium |
| 1 | No |
| 2 | No |
| 3 | No |
| 4 | No |
| 5 | No |
| 6 | No |
| 7 | No |
| 8 | No |
Claims (2)
1. a Simple soil bactericidal unit, it is characterized in that comprising large-scale boiling flask (1), first beaker (2), sample feeding pipe (3), first valve (4), second beaker (5), second valve (6), 3rd valve (7), first air filter (8), 4th valve (9), air pump (10), second air filter (11), polyethylene tube (12), triangular pyramidal bottle cap (13), 3rd air filter (14), first filter paper (15), triangular pyramidal bottle cap (16), second filter paper (17), 4th air filter (18), 5th valve (19), the triangular pyramidal bottle cap (20) of upper end open, 6th valve (21), iron wire (22), thermostat water bath (23), potassium permanganate (24), pedotheque (25), ammonia (26),
Large-scale boiling flask (1) is provided with in thermostat water bath (23), the first beaker (2) is provided with in large-scale boiling flask (1), first beaker (2) is built with potassium permanganate (24), first beaker (2) is fixed with iron wire (22), the gas feed of large-scale boiling flask (1) upper right quarter is provided with the 4th valve (9) in turn, first air filter (8), the liquid-inlet of large-scale boiling flask (1) upper left quarter is provided with sample feeding pipe (3), sample feeding pipe (3) one end is in the first beaker (2) top, sample feeding pipe (3) other end is provided with the first valve (4), large-scale boiling flask (1) top is provided with triangular pyramidal bottle cap (20), triangular pyramidal bottle cap (20), 6th valve (21), second air filter (11), triangular pyramidal bottle cap (16), second filter paper (17), polyethylene tube (12), first filter paper (15), triangular pyramidal bottle cap (13), 3rd air filter (14), air pump (10), 3rd valve (7), large-scale boiling flask (1) central gas import is connected in turn, pedotheque (25) is placed in polyethylene tube (12), by tee T and the 5th valve (19) between second air filter (11) and the 6th valve (21), 4th air filter (18) is connected, be connected with the second valve (6) by tee T between air pump (10) with the 3rd valve (7), second valve (6) below is provided with the second beaker (5), second beaker (5) is built with ammonia (26).
2. use a Simple soil sterilizing methods for device as claimed in claim 1, it is characterized in that its step is as follows:
1) add 5-10g potassium permanganate (24) by beaker (2), put into boiling flask (1); To complete by all sections fit except splendid attire soil sample parts, open thermostat water bath (23), control temperature is constant at 60-80 DEG C;
2) the second filter paper (17) is positioned in triangular pyramidal bottle cap (16), triangular pyramidal bottle cap (16) is tightened against in polyethylene tube (12), pedotheque (25) 30-50g crossing 1-2mm sieve is loaded in the polyethylene tube (12) tightening below lid, afterwards top triangular pyramidal bottle cap (13) having added the first filter paper (15) is tightened, be connected with the miscellaneous part assembled;
3) keep the second valve (6), the 4th valve (9), the 5th valve (19) to be in closed condition, the first valve (4), the 3rd valve (7), the 6th valve (21) stay open state; Add formalin 20-40ml by sample feeding pipe (3), close the first valve (4) rapidly, after 3-5 minute, large-scale boiling flask is full of formaldehyde gas in (1);
4) open air pump (10), formaldehyde gas circulation, after 20-30 minute, opens valve second valve (6), the 5th valve (19), and close the 3rd valve (7), the 6th valve (21), bleed 20-30 minute, to pump the formaldehyde existed in soil;
5) air pump (10) is closed, two ends are taken off together with the second air filter (11), splendid attire soil parts polyethylene tube (12) of the 3rd air filter (14), triangular pyramidal bottle cap (13), the first filter paper (15), triangular pyramidal bottle cap (16), the second filter paper (17), takes on aseptic operating platform for subsequent use;
6) other soil subject to sterilization is loaded in polyethylene tube, repeat step 2)-step 5), after all confession examination soil disinfections complete, after all samples sterilizing completes, close the first valve (4), 5th valve (19), 3rd valve (7), open the second valve (6), 4th valve (9), 6th valve (21), bleed 30-40 minute, formaldehyde gas imports the second beaker (5) that ammonia (26) is housed, by clean for formaldehyde sucking filtration in large-scale boiling flask (1), close thermostat water bath (23), after temperature reduces, first beaker (2) is taken out, clean the rear remaining residue of reaction.
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|---|---|---|---|
| CN201410629529.XA CN104383568B (en) | 2014-11-10 | 2014-11-10 | Simple soil bactericidal unit and sterilizing methods thereof |
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| CN201410629529.XA CN104383568B (en) | 2014-11-10 | 2014-11-10 | Simple soil bactericidal unit and sterilizing methods thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105651913A (en) * | 2016-02-29 | 2016-06-08 | 浙江大学 | Device and method for determining amino acid adsorption capacity of soil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2176775Y (en) * | 1993-05-18 | 1994-09-14 | 北京市安达医学工程开发公司 | Formol fumigating sterilizer |
| CN204261080U (en) * | 2014-11-10 | 2015-04-15 | 浙江大学 | A kind of Simple soil bactericidal unit |
-
2014
- 2014-11-10 CN CN201410629529.XA patent/CN104383568B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2176775Y (en) * | 1993-05-18 | 1994-09-14 | 北京市安达医学工程开发公司 | Formol fumigating sterilizer |
| CN204261080U (en) * | 2014-11-10 | 2015-04-15 | 浙江大学 | A kind of Simple soil bactericidal unit |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105651913A (en) * | 2016-02-29 | 2016-06-08 | 浙江大学 | Device and method for determining amino acid adsorption capacity of soil |
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| CN104383568B (en) | 2016-10-12 |
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