Summary of the invention
The technical problem that the present invention solves is to provide a kind of bacterio static itching-relieving promoting healing product containing Aloe extract and preparation method thereof, effectively can reduce the synthetic antiseptic use amount in product, effectively can improve the safety in utilization of the Related product of Aloe glue and other Aloe extracts.
For solving the problems of the technologies described above, the invention provides a kind of bacterio static itching-relieving promoting healing product containing Aloe extract, comprise water, hyaluronate sodium, glycyrrhizic acid dipotassium, Aloe Barbadensis Miller juice powder, glycerol, phenoxyethanol, caprylyl glycol, butanediol and glucosan; It is characterized in that, also comprise plant antiseptic agent;
Described plant antiseptic agent comprises Radix Et Rhizoma Rhei extract 18-22 weight portion, Cortex cinnamomi japonici (Ramulus Cinnamomi) extracting solution 34-38 weight portion, Herba Saxifragae extracting solution 13-18 weight portion, thorn Acanthopanan trifoliatus (L.) Merr. extracting solution 16-18 weight portion.
The above-mentioned bacterio static itching-relieving promoting healing product containing Aloe extract, also can have following features:
Each component raw material percentage ratio is: water is 72.00-73.00%, hyaluronate sodium is 0.26-0.30%, glycyrrhizic acid dipotassium 0.04-0.06%, Aloe Barbadensis Miller juice powder is 4.50-5.50%, glycerol is 4.50-5.50%, and phenoxyethanol and caprylyl glycol are 0.25-0.35%, and butanediol is 11.50%-12.50%, glucosan is 4.50-5.50%, and plant antiseptic agent is 0.04-0.06%.
The above-mentioned bacterio static itching-relieving promoting healing product containing Aloe extract, also can have following features:
Described each component raw material percentage ratio is: water is 72.32%, hyaluronate sodium is 0.28%, glycyrrhizic acid dipotassium 0.05%, Aloe Barbadensis Miller juice powder is 5.00%, glycerol is 5.00%, and phenoxyethanol and caprylyl glycol are 0.30%, and butanediol is 12.00%, glucosan is 5.00%, and plant antiseptic agent is 0.05%.
For solving the problems of the technologies described above, present invention also offers a kind of preparation method of the bacterio static itching-relieving promoting healing product containing Aloe extract, comprising the steps:
Step a: carry out disinfection production equipment cleaning operation;
Step b: water, hyaluronate sodium, glycyrrhizic acid dipotassium, Aloe Barbadensis Miller juice powder and glycerol are added in production equipment and obtains mixing material, and carry out heated and stirred operation; Wherein, whipping temp is 85 DEG C, and speed of agitator is 35r/min, and the persistent period is 30min;
Step c: when the mixing material temperature in production equipment is down to 75 DEG C, phenoxyethanol and caprylyl glycol being added in mixing material and to stir, mixing speed is 38r/min;
Steps d: when the mixing material temperature in production equipment is down to 45 DEG C, glucosan and plant antiseptic agent being added in mixing material and to stir, mixing speed is 32r/min;
Step e: when the mixing material temperature in production equipment is down to 38 DEG C, carries out sampling and detects operation, and mixing material is carried out the standing operation of discharging.
Technique scheme of the present invention has following beneficial effect:
The present invention is by adopting plant antiseptic agent, and the interpolation that effectively can reduce synthetic antiseptic uses, and effectively can improve the safety in utilization of the Related product of Aloe glue and other Aloe extracts.
Other features and advantages of the present invention will be set forth in the following description, and, partly become apparent from description, or understand by implementing the present invention.Object of the present invention and other advantages realize by structure specifically noted in description, claims and obtain.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearly understand, hereinafter will be described in detail to embodiments of the invention.It should be noted that, when not conflicting, the embodiment in the application and the feature in embodiment can combination in any mutually.
The invention provides a kind of bacterio static itching-relieving promoting healing product containing Aloe extract, comprise water, hyaluronate sodium, glycyrrhizic acid dipotassium, Aloe Barbadensis Miller juice powder, glycerol, phenoxyethanol, caprylyl glycol, butanediol and glucosan; It is characterized in that, also comprise plant antiseptic agent;
Described plant antiseptic agent comprises Radix Et Rhizoma Rhei extract 18-22 weight portion, Cortex cinnamomi japonici (Ramulus Cinnamomi) extracting solution 34-38 weight portion, Herba Saxifragae extracting solution 13-18 weight portion, thorn Acanthopanan trifoliatus (L.) Merr. extracting solution 16-18 weight portion.
It should be noted that, the preparation technology of each extracting solution above-mentioned the usual preparation technology of this area can carry out corresponding extraction; Now preparation technology is summarized as follows:
The preparation technology of Radix Et Rhizoma Rhei extract comprises and to be pulverized by Radix Et Rhizoma Rhei dry tablet and to cross 100 mesh sieves, and adds the ethanol extraction agent that concentration is 70%, carries out the ultrasonic Treatment operation of 35min; Carry out the operation of constant temperature reflux, extract, reflux temperature is 60 DEG C, and the time is 1h; Radix Et Rhizoma Rhei extract is obtained after finally extracting liq being carried out vacuum filtration, distilling under reduced pressure concentration operation;
The preparation technology of Cortex cinnamomi japonici (Ramulus Cinnamomi) extracting solution comprises and to be pulverized by Cortex cinnamomi japonici (Ramulus Cinnamomi) and to cross 60 mesh sieves, and adds the ethanol extract that concentration is 70%, and solid-liquid ratio is 1g:10ml; Filter after lixiviate under 60 DEG C of temperature conditions, after extraction time 8h, obtain filtrate, obtain Cortex cinnamomi japonici (Ramulus Cinnamomi) extracting solution by after filtrate evaporation and concentration; Cortex cinnamomi japonici (Ramulus Cinnamomi) extracting solution also can obtain with Rotary Evaporators is concentrated, and preparation technology is the state of the art, repeats no more herein;
The preparation technology of Herba Saxifragae extracting solution comprises and to be pulverized by Herba Saxifragae and to cross 50 mesh sieves, and to add concentration be 80% ethanol extract, solid-liquid ratio 1g:25ml; Under 50 DEG C of temperature conditions, carry out ultrasonic extraction operation, hyperacoustic ultrasonic power is 100W; Herba Saxifragae extracting solution is obtained after extraction time 35min;
The preparation technology of thorn Acanthopanan trifoliatus (L.) Merr. extracting solution comprises and to be pulverized by thorn Acanthopanan trifoliatus (L.) Merr. and to cross 50 mesh sieves, and adds the ethanol extract that concentration is 80%, and solid-liquid ratio is 1g:20ml; Carry out microwave extraction operation, microwave power is 200W; Thorn Acanthopanan trifoliatus (L.) Merr. extracting solution is obtained after extraction time 3min.
Preferably, the above-mentioned bacterio static itching-relieving promoting healing product containing Aloe extract, also can have following features:
Each component raw material percentage ratio can be: water is 72.00-73.00%, hyaluronate sodium is 0.26-0.30%, glycyrrhizic acid dipotassium 0.04-0.06%, Aloe Barbadensis Miller juice powder is 4.50-5.50%, glycerol is 4.50-5.50%, and phenoxyethanol and caprylyl glycol are 0.25-0.35%, and butanediol is 11.50%-12.50%, glucosan is 4.50-5.50%, and plant antiseptic agent is 0.04-0.06%.Preferably, above-mentioned glucosan is beta glucan.
Preferably, the concrete component of the above-mentioned bacterio static itching-relieving promoting healing product containing Aloe extract can be:
Water is 72.32%, and hyaluronate sodium is 0.28%, glycyrrhizic acid dipotassium 0.05%, Aloe Barbadensis Miller juice powder is 5.00%, and glycerol is 5.00%, and phenoxyethanol and caprylyl glycol are 0.30%, butanediol is 12.00%, and glucosan is 5.00%, and plant antiseptic agent is 0.05%.
Preferably, the concrete component of the above-mentioned bacterio static itching-relieving promoting healing product containing Aloe extract can be:
Water is 72.00%, and hyaluronate sodium is 0.26%, glycyrrhizic acid dipotassium 0.04%, Aloe Barbadensis Miller juice powder is 4.90%, and glycerol is 4.50%, and phenoxyethanol and caprylyl glycol are 0.25%, butanediol is 12.50%, and glucosan is 5.50%, and plant antiseptic agent is 0.05%
Preferably, the concrete component of the above-mentioned bacterio static itching-relieving promoting healing product containing Aloe extract can be
Water is 73.00%, and hyaluronate sodium is 0.30%, glycyrrhizic acid dipotassium 0.06%, Aloe Barbadensis Miller juice powder is 4.50%, and glycerol is 5.50%, and phenoxyethanol and caprylyl glycol are 0.35%, butanediol is 11.50%, and glucosan is 4.75%, and plant antiseptic agent is 0.04%.
In order to the anti-microbial property of above-mentioned plant antiseptic agent provided by the invention can be checked further, now carry out following corresponding experiment test by the usual antibacterial test experimental technique of this area:
Plant antiseptic agent: following several embodiment provided by the invention, in table 1;
The content of table 1: embodiment 1-5 illustrates (unit: weight portion)
Sequence number |
Component |
Embodiment 1 |
Embodiment 2 |
Embodiment 3 |
Component 1 |
Radix Et Rhizoma Rhei extract |
18 |
20 |
22 |
Component 2 |
Cortex cinnamomi japonici (Ramulus Cinnamomi) extracting solution |
34 |
36 |
38 |
Component 3 |
Herba Saxifragae extracting solution |
13 |
15 |
18 |
Component 4 |
Thorn Acanthopanan trifoliatus (L.) Merr. extracting solution |
16 |
17 |
18 |
Strain and condition of culture: adopt strain in specific experiment of the present invention, in table 2;
Table 2: strain explanation
Kind |
Culture medium |
Condition of culture |
Staphylococcus aureus |
Nutrient agar/PCA |
30℃/48h |
Escherichia coli |
Nutrient agar/PCA |
30℃/48h |
Pseudomonas |
False unit cell agar culture medium |
25℃/48h |
The dead circumfili bacterium of heat |
STAA agar culture medium, |
30℃/48h |
Filter paper experimental procedure is sketched;
Picking cultivates the bacterium colony 2 that 24-48h is of moderate size, and sterile working adds in 9ml physiological saline solution, uses magnetic stirrer 1min, and becoming uniform is 3-5X10 containing bacterium colony
6-10
7cfu.mL
-1outstanding bacterium liquid for subsequent use; The filter paper of thickness 1.5mm being broken into diameter is 6mm disk, and after being wrapped up by filter paper, 160 DEG C of dry heat sterilization 2h are for subsequent use; Be used for each selective medium heating and melting for examination bacterium by what prepare, be poured in too heat-killed culture dish, for subsequent use after flat board solidifies; Drawing 0.2mL respectively respectively for trying bacteria suspension in plate with aseptic straw, being coated with rod with sterile glass triangle and bacteria suspension is carefully coated with evenly; Filter paper is soaked 1min respectively in plant antiseptic agent embodiment 1-5, takes out cool micro-doing and be gently affixed on flat board; Then be inverted in calorstat by plate, each bacterium cultivates 24h according to optimum temperature; The diameter mm of each inhibition zone is measured with micrometer; Bacteria used thereby suspension is down flat plate numeration simultaneously.
Test data analysis; Plant antiseptic agent embodiment 1-5 experimental data is in table 3;
Table 3: embodiment 1-5 experimental data synopsis (unit mm)
Kind |
Embodiment 1 |
Embodiment 2 |
Embodiment 3 |
Staphylococcus aureus |
25.5 |
27.8 |
26.5 |
Escherichia coli |
23.7 |
26.4 |
23.9 |
Pseudomonas |
23.4 |
25.0 |
24.1 |
The dead circumfili bacterium of heat |
24.3 |
26.5 |
24.8 |
Blank group |
6.0 |
6.0 |
6.0 |
Learn thus, in plant antiseptic agent embodiment 1-3 provided by the invention, anti-microbial property is embodiment 2 preferably.
Inventor finds through retrieval: patent of invention (a kind of bioactive small molecule polypeptide keratin essence of Guangzhou You Mei biotechnology Co., Ltd application, application number: 201410280021.3), record plant antiseptic agent, it specifically comprises: Radix Et Rhizoma Rhei extract 20-40 weight portion, Pericarpium Granati extracting solution 10-25 weight portion, magnolia officinalis rehd.et wils.var.biloba rehd.et wils. extracting solution 10-25 weight portion, Herba Saxifragae extracting solution 10-35 weight portion, thorn Acanthopanan trifoliatus (L.) Merr. extracting solution 10-15 weight portion; And the patent of invention of Tianjin Yumeijing Group Co., Ltd's application (a kind of cosmetics containing plant bacteriostatic composition, application number is: 201210126080.6), record plant bacteriostatic composition, it specifically comprises: Radix Glycyrrhizae extract, Flos Lonicerae extract magnolia obovata extract.
In order to verify the above-mentioned antiseptic and inhibiting bacteria function performance containing plant antiseptic agent in the bacterio static itching-relieving promoting healing product of Aloe extract provided by the invention further, now carry out control comparison experiment (documents 1, a kind of bioactive small molecule polypeptide keratin essence in conjunction with plant antiseptic agent of the prior art; Documents 2, a kind of cosmetics containing plant bacteriostatic composition).The bacteriostatic preservative that documents 1, documents 2, the embodiment of the present application 2 choose same volume concentration all separately carries out corresponding control experiment.Repeat anti-microbial property experimental procedure, draw the application and existing antiseptic check analysis data, in table 4;
Table 4: the application and existing antiseptic check analysis table (unit mm)
According to upper table; In the application, its antibacterial effect, significantly better than the antibacterial effect of documents 1, documents 2, draws the concrete outcome of contrast, in table 5 to the plant antiseptic agent embodiment that the application contains;
Table 5: the contrast ratio (with documents 1 fungistatic effect for 100%) of check analysis table
According to upper table; The antibacterial effect of the plant antiseptic agent that the application contains exceeds more than 34% compared with 1 fungistatic effect of documents, exceeds more than 22% compared with 2 fungistatic effects of documents.
In summary; The above-mentioned plant antiseptic agent embodiment that the application contains, by Radix Et Rhizoma Rhei, Cortex cinnamomi japonici (Ramulus Cinnamomi), stone, Herba Saxifragae and the creationary combination matching of thorn Acanthopanan trifoliatus (L.) Merr., the final antibacterial effect realized is far superior to the antibacterial effect of plant antiseptic agent of the prior art.Meanwhile, relative to documents 1, the magnolia officinalis rehd.et wils.var.biloba rehd.et wils. market price that the Cortex cinnamomi japonici (Ramulus Cinnamomi) component market price contained by the application contains lower than documents 1; The application, while raising fungistatic effect, can reduce the fund input of each component to a certain extent, can effectively for enterprise creates more value.
Accordingly, present invention also offers a kind of preparation method of the bacterio static itching-relieving promoting healing product containing Aloe extract, comprise the steps:
Step a: carry out disinfection production equipment cleaning operation;
Step b: water, hyaluronate sodium, glycyrrhizic acid dipotassium, Aloe Barbadensis Miller juice powder and glycerol are added in production equipment and obtains mixing material, and carry out heated and stirred operation;
Wherein, whipping temp is 85 DEG C, and speed of agitator is 35r/min, and the persistent period is 30min;
Step c: when the mixing material temperature in production equipment is down to 75 DEG C, phenoxyethanol and caprylyl glycol being added in mixing material and to stir, mixing speed is 38r/min;
Steps d: when the mixing material temperature in production equipment is down to 45 DEG C, glucosan and plant antiseptic agent being added in mixing material and to stir, mixing speed is 32r/min;
Step e: when the mixing material temperature in production equipment is down to 38 DEG C, carries out sampling and detects operation, and mixing material is carried out the standing operation of discharging.
Through final its standard of product that obtains of above-mentioned steps preparation with reference to QB/T 2874-2007 skin protection gel standard; Corresponding Standard and the application finally obtain the index results of comparison of product, in table 6;
Table 6: the index results of comparison of Standard and the application's product
According to upper table; The product that the application is finally obtained by above-mentioned preparation method, its property indices all in national standard, the farther corresponding index far above national requirements of its sanitary index; Concrete, the application is by adopting the obvious plant antiseptic agent of fungistatic effect, finally realize total number of bacteria≤50CFU/g (national standard is≤1000CFU/g), yeast and mold sum≤10CFU/g (national standard is≤100CFU/g).
In summary; Above-mentioned a kind of bacterio static itching-relieving promoting healing product containing Aloe extract that the application provides, the interpolation that effectively can reduce synthetic antiseptic uses, and effectively can improve the safety in utilization of the Related product of Aloe glue and other Aloe extracts; Accordingly, above-mentioned preparation method is compared with preparation method of the prior art, and preparation technology is rationally simple and direct; And its property indices of the product drawn is far away higher than the corresponding index of national requirements, has good beneficial effect, easy to spread and implement.
Although it is apparent to those skilled in the art that embodiment disclosed by the present invention as above, the embodiment that described content only adopts for ease of understanding the present invention, and be not used to limit the present invention.Those of skill in the art belonging to any the present invention; under the prerequisite not departing from the spirit and scope disclosed by the present invention; any amendment and change can be carried out in the form implemented and details; but scope of patent protection of the present invention, the scope that still must define with appending claims is as the criterion.