CN104381123A - Screening method of G. hirsutum race with high glyphosate material sensibility - Google Patents
Screening method of G. hirsutum race with high glyphosate material sensibility Download PDFInfo
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- CN104381123A CN104381123A CN201410532239.3A CN201410532239A CN104381123A CN 104381123 A CN104381123 A CN 104381123A CN 201410532239 A CN201410532239 A CN 201410532239A CN 104381123 A CN104381123 A CN 104381123A
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Abstract
The invention belongs to agricultural pharmacology and relates to a screening method of G. hirsutum race with high glyphosate material sensibility. The screening method comprises the following steps of 1, carrying out local treatment on a G. hirsutum race natural population by a low-concentration herbicide, and recognizing a single plant with high herbicide sensibility, 2, selecting the single plant with high herbicide sensibility, carrying out selfing, and carrying out plant division harvesting and storage, wherein a part of the seeds are used for breeding and a part of the seeds are used for identification, and 3, orderly carrying out plant sowing and seedling emergence, treatment by a reagent, sensibility and resistance identification, corresponding high-sensibility plant selection, selfing, and plant division harvesting and storage, and repeating the above processes three times to obtain the G. hirsutum race material with high herbicide sensibility.
Description
Technical field
The invention belongs to Pesticide Science, be specifically related to the screening technique of Gossypium hirsutum L. Races height sense glyphosate material.
Background technology
Be similar to insecticide, bactericide, after weed killer herbicide comes out 15 years, weeds produce antiweed successively, so far, nearly 450 kinds of antiweed weeds are had to be found to be distributed in all over the world, countries in the world scientist is also finding the biology of high antiweed always, and is devoted to study its resistance mechanism, and then researches and develops various Resistant Herbicide Crops.But, more and more the Selective Pressure of high concentration herbicide accelerates Weed Resistance evolution, and existing resistance mechanism can not explain the resistance level of weeds completely, therefore be also preferably Research Thinking from starting with to the biology of herbicide sensitive, and screening is the most important thing to the biology of herbicide sensitive.
Gossypium hirsutum L. Races (G.hirsutum race), cotton also known as semi-wild, be the upland cotton without domestication, area of origin geographical conditions are complicated, climate variability, and cotton belongs to Constantly allogamous, therefore have and enrich variation type, contain the gene that many cultivated speciess do not possess.From Gossypium hirsutum L. Races, screen high resistance or high sense weed killer herbicide materials application in the research of cotton induction reactance weed killer herbicide, both effectively can utilize the objective trait to weed killer herbicide induction reactance, and also can enrich the narrow hereditary basis of current cotton variety, optimum way of can yet be regarded as.
Also there is no the report of programmed screening herbicide sensitive MATERIALS METHODS at present, according to the weed killer herbicide certain seedling age material being sprayed to the gradient concentration of setting, close to Production of Large Fields process, but the experimental size required is larger, consume a large amount of seed, and be treated to crushing process, easily make plant lethal, lose precious material, therefore be not suitable for screening large group, limited or rare material.The qualification of seed germination method and flower bud phase growing point spot printing method, all may to arrange the improper material that makes lethal because of concentration, and go out target material by mistake.The qualification of blade cotton balls location, precisely effectively, but differs larger with the processing mode of field weeding.
The present inventor notices, by the herbicide solution of the gradient concentration arranged, Local treatment is carried out to cotton material, Preliminary Identification goes out sensitive plants, selfing breeding, sowing, sow, emerge after spray the weed killer herbicide of herbicide concentration, continue screening, breeding simultaneously, circular treatment several generations, can obtain stable height sense weed killer herbicide cotton plant.
Summary of the invention
The object of this invention is to provide the method for high sense glyphosate material in screening Gossypium hirsutum L. Races.
According to the method for sense weed killer herbicide material high in screening Gossypium hirsutum L. Races of the present invention, comprise the following steps:
(1) with weed killer herbicide, Local treatment is carried out to non-Bt cotton kind, find out the herbicide concentration that reaction appears in normal conon, with this concentration for maximum concentration, separately establish some gradient low concentration liquids successively, the least concentration arranged can not make all material occur reaction, Local treatment is carried out to the natural population of Gossypium hirsutum L. Races, in colony, identifies the individual plant of the Gossypium hirsutum L. Races material of high sense weed killer herbicide;
(2) the height sense individual plant material selected carries out selfing, plant division is picked, pack, cotton ginning, storage;
(3) sow in units of every strain, emerge, adopt the weed killer herbicide of herbicide concentration to spray the height sense weed killer herbicide Gossypium hirsutum L. Races material filtered out, carry out susceptibility identification, by identifying the breeding cotton plant selfing of the corresponding strain of high sense material, continue to harvest and store by strain;
(4) repeat step (3), finally obtain the Gossypium hirsutum L. Races of high sense weed killer herbicide.
According to the specific embodiment of the present invention, first carry out Local treatment with weed killer herbicide to Gossypium hirsutum L. Races natural population, identify the individual plant of high sense in colony, selfing also divides receipts selfing bell, a seed part for results is used for breeding, and a part is used for susceptibility qualification.Then the cyclic program of " insemination and emergence-chemicals treatment-induction reactance identification-responsive strain selection-selfing-point contracture storage " is entered, the last material filtering out high sense weed killer herbicide from Gossypium hirsutum L. Races natural population, result shows: the cotton 80G (Latifolium80G) of broad-leaved and the cotton 80AG (Latifolium80AG) of broad-leaved are the Gossypium hirsutum L. Races materials of high sense glyphosate; Major part Gossypium hirsutum L. Races is extremely responsive to careless ammonium phosphine.
According to the screening technique of Gossypium hirsutum L. Races height sense weed killer herbicide of the present invention, comprise the following steps:
1) with weed killer herbicide, Local treatment is carried out to non-Bt cotton kind, find out the herbicide concentration that reaction appears in normal conon, with this concentration for maximum concentration, separately establish several gradient low concentration (least concentration of setting would not make all material occur reaction) liquid successively, Local treatment is carried out to experiment material, finds out the Gossypium hirsutum L. Races material of high sense;
2) to the height sense material selfing filtered out, a point individual plant is picked, and pack, cotton ginning, storage respectively;
3) seed of every strain results takes out a part and gives over to breeding, remainingly carries out susceptibility qualification.Sow in units of every strain, emerge, adopting the weed killer herbicide of herbicide concentration to spray the height sense weed killer herbicide Gossypium hirsutum L. Races material filtered out, carry out susceptibility identification, by identifying the breeding cotton plant selfing of the corresponding strain of high sense material, continuing to harvest and store by strain.
4) according to step 3) carry out circulation three generations, finally obtain the Gossypium hirsutum L. Races of high sense weed killer herbicide.
The technology that the present invention relates in basic scientific research cause tool significance, the following advantage of its tool:
1, the target material that by mistake goes out is avoided, optimize and widen its embodiment, in conjunction with the processing method of land for growing field crops herbicide spraying weeding, greatly reduce the workload of screening, it also avoid the phenomenon of " creating groundless rumors " and " narrowly missing ", contact field to produce simultaneously, and ensure that accurately reliable result.
2, method screening of the present invention obtains the cotton 80G of Gossypium hirsutum L. Races material broad-leaved and the cotton 80AG. of broad-leaved of high sense herbicide glyphosate, for cotton provides control material to the qualification of herbicide glyphosate susceptibility, also provide material foundation for the glyphosate genetic research of cotton induction reactance and induction reactance Mechanism Study.
3, in view of the resistance mechanism of the many resistant weeds found at present and resistance level inconsistent, to the cotton 80G of broad-leaved of herbicide glyphosate height sense and the cotton 80AG of broad-leaved in the present invention, for research of starting with from height sense material explains that this phenomenon provides basic material.
4, adopt the weed killer herbicide of high concentration that the antiweed weeds frequency of occurrences is increased greatly, with the herbicide treatment height sense weed killer herbicide material of low dosage, greatly reduce, even eliminate the impact on environment.
5, by measuring induction reactance material shikimic acid content, fresh weight/dry weight, chlorophyll content, glutathione-S-transferase (GSTs) activity etc., study or supplement it to the sense resistance mechanism of weed killer herbicide.
6, the screening of Gossypium hirsutum L. Races height sense weed killer herbicide material, has widened the hereditary basis of current cultivar, has enriched biological genetic diversity.
Accompanying drawing explanation
The cotton leaf of Fig. 1 local drug delivery process, on cotton leaf, 1-4 herbicide concentration is followed successively by 0.09%, 0.03%, 0.006%, 0.0015%.
The cotton leaf result of Fig. 2 local drug delivery process, a. CCRI 12, dispenser 0.09% responds; B. broad-leaved cotton 80G height sense individual plant, dispenser 0.006% responds, and 0.0015% is reactionless.
Fig. 3 cotton sprays glyphosate result, c. CCRI 12 dispenser 10 days; D. broad-leaved cotton 80G height sense strain dispenser 10 days; E. CCRI 12 dispenser 20 days.
Embodiment
Embodiment 1 screens high sense herbicide glyphosate Gossypium hirsutum L. Races
1 materials and methods
1.1 experiment material
Gossypium hirsutum L. Races, totally 388 parts (referring to table 1), draw from American National Cotton Germplasms storehouse, specifically comprise that 33 parts of sharp spots are cotton, 43 parts of not cotton and 12 parts of Pa Moer cottons of cotton, the 86 parts of Ma Lijialangte cottons in cotton, the 4 parts of Yucatan cottons of rel, 11 parts of Lei Qimeng ground, 199 parts of broad-leaveds.Contrast is CCRI 12, provides by the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute.Herbicide glyphosate is the glyphosate isopropyl amine salt solution that Monsanto Company produces, and active constituent content is 41%.
Table 1 experiment material numbering and title
1.2 experimental technique
1.2.1 herbicide glyphosate liquid prepares (, for 820ml, working solution is for 1L for mother liquor)
1) mother liquor: mother liquid concentration is 0.1%.Get the former medicine of 2ml glyphosate isopropyl amine salt, be settled to 800ml, then add 20ml water, mixing, leaves standstill to abundant mixing, stand-by.
2) working solution preparation: get 15ml, 60ml, 300ml, 900ml respectively in suitable beaker, be settled to 1L, be mixed with the glyphosate working solution of 0.0015%, 0.006%, 0.03%, 0.09%, mixing, leaves standstill, stand-by.
1.2.2 screening process:
(1) with 0.0015%, 0.006%, 0.03% and 0.09% glyphosate working solution, to normal conon kind CCRI 12, CCRI 16, CCRI 49 and CCRI 79, carry out the process of partial smearing liquid, investigation result is: normal conon kind occurs poisoning under the glyphosate concentration of 0.09%.Meanwhile, herbicide glyphosate test herbicide concentration is 0.1%.
(2) with 0.0015%, 0.006%, 0.03% and 0.09% glyphosate working solution local drug delivery, process Gossypium hirsutum L. Races natural population, totally 388 parts, filter out high sense glyphosate material 2 parts: the cotton 80G of broad-leaved and the cotton 80AG of broad-leaved, in table 1, respective material numbering is respectively: 266 and 267.
(3) selfing carried out to the height sense individual plant selected, plant division is picked, pack, point to roll, point storage.A part of seed of material is used for breeding, and remainder is used for susceptibility qualification, according to strain sowing, and the CCRI 12 of plantation contrast simultaneously.
(4) with the glyphosate liquid of herbicide concentration after emerging, the program of " medicament sprays process-susceptibility identification-corresponding strain selection-selfing-point contracture storage " to the cotton 80G of the broad-leaved of two high sense herbicide glyphosates and broad-leaved cotton 80AG strain material, is implemented.
(5) program " sowing-emerge-chemicals treatment-susceptibility identification-corresponding strain selection-selfing-point contracture storage by strain ", circulates 3 times, from Gossypium hirsutum L. Races natural population, finally screens the material of stable height sense herbicide glyphosate.
Local drug delivery is identified high sense material reaction density (0.006%) and is sprayed and identifies that concentration (0.1%) differs greatly, and herbicide concentration is the concentration of medium glyphosate tolerant cotton sensitivity, if arrange several gradient concentration liquid lower than herbicide concentration directly spray expert evidence, result is that the action effect difference of storeroom is not obvious, can not accurately distinguish and evaluate high sense and sensitive material, easily occur mistake that is groundless and that narrowly miss.Therefore, adopt the sensitivity levels of local drug delivery accurate evaluation every individual plant material, it also avoid and directly spray destruction material, combine simultaneously and produce close land for growing field crops and spray herbicide concentration method, can the resistance level of actual response material.
Claims (4)
1. the screening technique of Gossypium hirsutum L. Races height sense glyphosate material, is characterized in that, said method comprising the steps of:
(1) with weed killer herbicide, Local treatment is carried out to non-Bt cotton kind, find out the herbicide concentration that reaction appears in normal conon, with this concentration for maximum concentration, separately establish some gradient low concentration liquids successively, the least concentration arranged can not make all material occur reaction, Local treatment is carried out to the natural population of Gossypium hirsutum L. Races, in colony, identifies the individual plant of the Gossypium hirsutum L. Races material of high sense weed killer herbicide;
(2) the height sense individual plant material selected carries out selfing, plant division is picked, pack, cotton ginning, storage;
(3) sow in units of every strain, emerge, adopt the weed killer herbicide of herbicide concentration to spray the height sense weed killer herbicide Gossypium hirsutum L. Races material filtered out, carry out susceptibility identification, by identifying the breeding cotton plant selfing of the corresponding strain of high sense material, continue to harvest and store by strain;
(4) repeat step (3), finally obtain the Gossypium hirsutum L. Races of high sense weed killer herbicide.
2. the screening technique of Gossypium hirsutum L. Races height sense glyphosate material according to claim 1, is characterized in that, repeat 3 times in step (3).
3. be applied to cotton in the qualification of herbicide glyphosate susceptibility from the cotton 80G of broad-leaved of Gossypium hirsutum L. Races height sense herbicide glyphosate and the cotton 80AG of broad-leaved as sensitivity controls.
4. be applied to the herbicide glyphosate genetic research of cotton induction reactance and induction reactance Mechanism Study from the cotton 80G of broad-leaved of Gossypium hirsutum L. Races height sense herbicide glyphosate and the cotton 80AG of broad-leaved as sense source parent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111820094A (en) * | 2020-07-24 | 2020-10-27 | 石家庄市农林科学研究院 | Planting method for shortening breeding period of green Chinese onions |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253472A (en) * | 1997-04-28 | 2000-05-17 | 严文贵 | Crop heterosis and herbicide |
| CN1541270A (en) * | 2000-09-29 | 2004-10-27 | Herbicide resistant plants | |
| AU2007202265A1 (en) * | 1998-03-09 | 2007-06-07 | Monsanto Technology Llc | Mixtures for weed control in glyphosate tolerant soybeans |
| CN102165885A (en) * | 2010-12-15 | 2011-08-31 | 中国农业科学院植物保护研究所 | Method for identifying glyphosate resistance of cotton |
| CN103364355A (en) * | 2013-07-19 | 2013-10-23 | 中国农业科学院棉花研究所 | Method for rapidly evaluating glyphosate tolerance of cotton |
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- 2014-10-11 CN CN201410532239.3A patent/CN104381123B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253472A (en) * | 1997-04-28 | 2000-05-17 | 严文贵 | Crop heterosis and herbicide |
| AU2007202265A1 (en) * | 1998-03-09 | 2007-06-07 | Monsanto Technology Llc | Mixtures for weed control in glyphosate tolerant soybeans |
| CN1541270A (en) * | 2000-09-29 | 2004-10-27 | Herbicide resistant plants | |
| CN102165885A (en) * | 2010-12-15 | 2011-08-31 | 中国农业科学院植物保护研究所 | Method for identifying glyphosate resistance of cotton |
| CN103364355A (en) * | 2013-07-19 | 2013-10-23 | 中国农业科学院棉花研究所 | Method for rapidly evaluating glyphosate tolerance of cotton |
Non-Patent Citations (3)
| Title |
|---|
| G. DINELLI,ET AL.: "Physiological and molecular insight on the mechanisms of resistance to glyphosate in Conyza canadensis (L.) Cronq. biotypes", 《PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY》, vol. 86, 31 December 2006 (2006-12-31), pages 30 - 41, XP 024897195, DOI: doi:10.1016/j.pestbp.2006.01.004 * |
| 付远志等: "利用种子发芽试验检测抗草甘膦棉花新品种", 《种子》, vol. 30, no. 9, 30 September 2011 (2011-09-30), pages 102 - 103 * |
| 高越等: "抗草甘膦野生大豆筛选及其抗性生理机制研究", 《大豆科学》, vol. 32, no. 1, 28 February 2013 (2013-02-28), pages 76 - 79 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111820094A (en) * | 2020-07-24 | 2020-10-27 | 石家庄市农林科学研究院 | Planting method for shortening breeding period of green Chinese onions |
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