CN102577857B - Method for identifying herbicide resistance of cotton leaves by cotton ball positioning - Google Patents
Method for identifying herbicide resistance of cotton leaves by cotton ball positioning Download PDFInfo
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- CN102577857B CN102577857B CN 201210019682 CN201210019682A CN102577857B CN 102577857 B CN102577857 B CN 102577857B CN 201210019682 CN201210019682 CN 201210019682 CN 201210019682 A CN201210019682 A CN 201210019682A CN 102577857 B CN102577857 B CN 102577857B
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Abstract
The invention relates to plant physiology and pesticide science, in particular to a method for identifying herbicide resistance of cotton leaves by cotton ball positioning, which includes the steps: selecting material leaves for treatment, namely selecting intact functional leaves or leaves adjacent to those; secondly, cotton ball positioning, namely setting action spots on the front face of every functional leaf of each cotton plant clockwise or anticlockwise from a stipe, dipping cotton balls approximately in the same size with herbicide solutions having set concentrations, and sequentially placing the cotton balls dipped with the solutions having different concentrations at the corresponding action spots on the surface of each leaf; thirdly, observing action conditions of the treated spots to determine resistance levels of the spots. By the method, materials can be saved greatly, experimental scale can be reduced, artificial errors can be reduced greatly, results are precise and reliable, precision identification of the material resistance levels is technically guaranteed, and deep resistance research on current-generation material single plants is guaranteed. The method is quite simple in operation and easy to master.
Description
Technical field
The present invention relates to plant physiology and Pesticide Science, particularly, the present invention relates to the method for cotton leaf cotton balls location evaluation Herbicid resistant.
Background technology
at present, it is usually the seed germination survey method that the cotton Herbicid resistant is identified, different times variable concentrations weed killer herbicide spraying method, flower bud phase blade spot printing growing point method, the culture herbicide treatment methods such as cell-line etc., but the seed germination survey method requires grain weight enough large, and spraying rule, different times variable concentrations weed killer herbicide require each seedling amount larger in period, also can be because spraying inequality, the elegant experimental error that causes of liquid, in addition in flower bud phase blade spot printing growing point method, growing point cannot represent the level of growth of whole cotton plant, and the higher concentration processing can cause that growth rate of cotton plant point is controlled, severe deformities, cotton plant can not blossom and bear fruit, and above the whole bag of tricks all may be lethal with material, and, when at the same time lot of materials being identified, require experimental size larger, be not easy to realize.
Simultaneously, when limited or rare cotton material being carried out the Herbicid resistant evaluation, due to the material quantitative limitation, process according to above-mentioned each method and very likely can destroy material, can not guarantee to find resistance level or the lethal concentration of material.At this moment, effective method is cotton plant to be carried out the part identify, so both can find the resistance level of material, can preserve material again.The present inventor recognizes, the functional leaf of cotton can represent the upgrowth situation of cotton plant, can process the cotton plant functional leaf and carry out cotton to the evaluation of Herbicid resistant.
Summary of the invention
The present inventor explores cotton plant Herbicid resistant authentication method for a long time, find to utilize the weed killer herbicide of variable concentrations to process simultaneously the cotton plant functional leaf, but both fast accurate is found out the resistance level of cotton plant, can continue again to preserve, the limited or rare cotton material of breeding; The Herbicid resistant that both had been applicable to a large amount of cotton materials is identified, also is applicable to the Herbicid resistant evaluation of limited or rare material, i.e. cotton balls location survey method.
Therefore, the method that the purpose of this invention is to provide cotton leaf cotton balls location evaluation Herbicid resistant.
Method according to cotton leaf cotton balls of the present invention location evaluation Herbicid resistant comprises the following steps:
1) selection of material processed blade: select excellent functional leaf or its ortho position leaf without the phenomenon of being injured;
2) cotton balls localization process: in the functional leaf front of every strain from petiole clockwise or counterclockwise set active position, the cotton balls that size is basically identical dips in the weed killer herbicide working solution of full setting concentration, sequentially places the cotton balls of various concentration medicaments immersions in the respective action position of blade surface by fixed-direction;
3) observe the treatment sites response situation and determine its resistance level.
According to the method for cotton leaf cotton balls of the present invention location evaluation Herbicid resistant, the step that it comprises cotton balls location evaluation wherein, is treated to Local treatment.According to requirement of the present invention, the blade of herbicide treatment is chosen functional leaf or its ortho position leaf, be generally down three or fall four leaves, it is the strongest blade of photosynthesis, because conditioned response is responsive to external world to cross tender tissue, when using the low concentration glyphosate, very easily show symptom of chemical damage, can not represent the resistance level of material; And the old tissue metabolism is not vigorous excessively, processes rear blade and may not react, and the natural Physiology and biochemistry of some of leaf tissue changes also easily affects qualification result.
Method according to cotton leaf cotton balls of the present invention location evaluation Herbicid resistant, require higher to weather conditions, guarantee to process rear 6 hours without rain, and be preferably in and process during without dew the calm sunny morning, guarantee that liquid and the blade of accurate concentration fully acts on; Too strict to do not need requiring breeding time of cotton plant, namely seedling stage, flower bud phase, flowering and boll-setting period all can.
The evaluation of cotton balls positioning mode according to the present invention to the cotton Herbicid resistant, wherein, absorbent cotton will be placed according to fixing order, or clockwise, or counterclockwise; It is sufficient that the absorbent cotton dose is wanted, but will guarantee cotton balls to be placed in blade and do not have liquid to flow; The cotton balls liquor strength can not unrestrictedly increase, otherwise may cause death of seedling or growing point deformity, and is seriously controlled; The absorbent cotton size is suitable, is sure not to occur variable concentrations absorbent cotton adhesion phenomenon; Cotton balls will use absorbent cotton, guarantees water imbibition.
According to the specific embodiment of the present invention, the method for described cotton leaf cotton balls location evaluation Herbicid resistant specifically comprises: the 1) selection of material processed blade: select excellent functional leaf without the phenomenon of being injured, a strain a slice; 2) the cotton balls positioning mode is processed, begin clockwise direction in the functional leaf front of every strain from the petiole place and set 5 positions, dip in the weed killer herbicide working solution of full setting concentration with the tweezers cotton balls that size is basically identical (absorbent cotton), sequentially be positioned over the blade surface relevant position.3) investigation in the 4th day after processing is observed the treatment sites response situation and is determined its resistance level.
The present invention is important technology invention in the basic scientific research cause, and international and domestic for creating, it has the following advantages:
1, spraying processing meeting is because spraying inequality, the legal adopted material resistance level of Pyatyi poisoning, can because visually observing, estimate that injured area or the order of severity difference of being injured are forbidden and causes human error, the definition meetings such as planting percent to select the improper experimental error that causes because of control time; Whether the cotton balls positioning mode is pressed the concentration classification, react as standard definition material resistance take treatment sites, greatly reduces human error, and result is accurately reliable.
2, for limited or rare material, adopt the cotton balls positioning mode to identify, for continuing normal growth, material provides guarantee, provide technical guarantee to explication material resistance level, also for carrying out deep resistance research, material individual plant in the present age gives security.
3, for the screening of lot of materials to Herbicid resistant, adopt the cotton balls positioning mode to identify and can greatly save material, reduce experimental size.
4, described method is quick than other method, and the cycle is short, general method will be after processing investigation in 10-15 days, but result namely appearred in cotton balls location survey method in 4 days, this has greatly reduced qualification cycle, has effectively improved determination rates.
5, described method can arrange several variable concentrations simultaneously on a strain cotton plant, and single treatment can be determined the reaction interval of cotton plant, greatly saves material, shortens qualification time, improves determination rates, has shown that the method is quick, economizes material, advantage in batches.
When 6, adopting the cotton balls positioning mode to carry out the weed killer herbicide evaluation to lot of materials, not only can know each material resistance level, simultaneously can be at comparative material Resistant Difference under same level.
7, dosage is few, significantly reduces experimental cost, and is substantially pollution-free to environment simultaneously, and huge contribution is done in environmental protection.And, operate extremely simply, easily grasp.
Description of drawings
Fig. 1 cotton balls positioning mode is processed cotton leaf, and on cotton leaf, 1-5 weed killer herbicide concentration increases successively.
Fig. 2 cotton balls location survey method observed result is the paramount sense of high resistance from a-f, wherein, a. high resistance, blade is reactionless, 1 grade; B. anti-, 5 reactions, 1-4 does not react, 2 grades; C. anti-, 4-5 responds, and 1-3 is reactionless, 3 grades; D. not anti-, 3-5 responds, and 1-2 is reactionless, 4 grades; E. sense, 2-5 responds, and 1 is reactionless, 5 grades; F. high sense, 1-5 has significant reaction, 6 grades.
Embodiment
1, processing material blade selects
Material plantation, management etc. do not have specific requirement, and the material of general planting management or self-sow all can.Blade selects there is no specific (special) requirements from the cotton plant position, stem, vegetative shoot, fruit branch, secondary or three grades of branches all can, but require excellent functional leaf without the phenomenon of being injured, a strain a slice; , be not strict with on breeding time from cotton plant, seedling stage, flower bud phase, flowering and boll-setting period all can.Before processing, blade is carried out mark, but the mark time can not be oversize in advance, otherwise for herbicide treatment, leaf growth is too fast, blade can be partially old, and error appears in result.
2, liquid preparation
At first liquid preparation is mixed with stoste mother liquor (every 10 milliliter of 41% glyphosate stoste fully be dissolved in be mixed with mother liquor in 400 ml waters be 1% glyphosate solution) by a certain percentage, further be diluted to again working solution (respectively 1% glyphosate mother solution of 6 milliliters, 30 milliliters, 90 milliliters, 150 milliliters and 200 milliliters fully is dissolved in the water of 994 milliliters, 970 milliliters, 910 milliliters, 850 milliliters and 800 milliliters, is mixed with respectively 0.006%, 0.03%, 0.09%, 0.15% and 0.2% glyphosate working solution).Stoste, mother liquor preferably are stored in refrigerator, and mother liquor and working solution can not preserve for a long time, and working solution can not be exposed to the sun in the sun, also can not be placed in the air port, and is preferably In Shade.
3, cotton balls localization process
The cotton balls localization process is to begin clockwise direction in the functional leaf front of every strain from the petiole place to set several positions, will dip in the cotton balls (absorbent cotton) of full setting concentration glyphosate with tweezers, sequentially is positioned over the blade surface relevant position, sees accompanying drawing 1.Key is that the cotton balls size is basically identical, and the absorbent cotton dose is sufficient, but not too many in case flow on blade.In addition, use blunt nosed tweezers, in order to avoid the tip tweezers scratch blade.
4, investigation
Investigation in about the 4th day after processing, response situation clearly with material resistance grading evaluation, is seen accompanying drawing 2 according to response situation, a. high resistance, blade is reactionless, 1 grade; B. anti-, 5 reactions, 1-4 does not react, 2 grades; C. anti-, 4-5 responds, and 1-3 is reactionless, 3 grades; D. not anti-, 3-5 responds, and 1-2 is reactionless, 4 grades; E. sense, 2-5 responds, and 1 is reactionless, 5 grades; F. high sense, 1-5 has significant reaction, 6 grades.
When cotton is identified in the cotton balls location; every cotton plant is the processing capacity leaf all; have uniformity and representativeness; and every functional leaf is all processed ad-hoc location, and is pointed, and the dose of the accurate concentration of every treatment sites is enough sufficient; the liquid that can guarantee every processing position tissue and accurate concentration fully acts on; and inequality usually can appear spraying in spraying, every material repeats to spray the phenomenon that dose differs in size, the variable concentrations liquid is elegant, causes the method to have randomness, difficulty to control, and error is larger.In addition, investigate every processing position when the cotton balls positioning mode is processed four days and reaction definition resistance level whether occurs, it is qualitative survey, artificial subjective error is less, the investigation of Pyatyi poisoning method will be estimated injured area or extent of injury seriousness, it is quantitative interview, artificial subjective error is larger, may be subjected to macroscopic the impact, and the spray liquid uniformity is very large on the result impact, the planting percent investigation method can more difficult unified dose level difference causes when processing delay of response, serious different, thereby control time hold the improper experimental error that causes.
Identify just to the local dispenser of cotton plant cotton balls location, and dose is very little, although herbicide glyphosate and phosphine oxamate have certain conductivity, the excessive concentration weed killer herbicide in cotton balls location only otherwise on cotton plant just is unlikely to suppress fully or lethal cotton plant.Therefore, the cotton plant after this method authentication process can be grown by normal growth substantially.
Claims (5)
1. the method for Herbicid resistant is identified in cotton leaf cotton balls location, it is characterized in that, said method comprising the steps of:
1) selection of material processed blade: select excellent functional leaf or its ortho position leaf without the phenomenon of being injured;
2) cotton balls localization process: begin clockwise from the petiole place in the functional leaf front of every strain or counterclockwise set active position, the cotton balls that size is basically identical dips in the weed killer herbicide working solution of full setting concentration, sequentially place the cotton balls of various concentration medicaments immersions in the respective action position of blade surface by fixed-direction, wherein, the weed killer herbicide dose that soaks of cotton balls reaches the standard that cotton balls that liquid soaks flows without liquid of placing on blade;
3) observe the treatment sites response situation and determine its resistance level.
2. method according to claim 1, is characterized in that, described material processed blade is the strongest blade of photosynthesis.
3. method according to claim 1, is characterized in that, carries out during without dew in calm, the sunny morning when processing cotton plant.
4. method according to claim 1, is characterized in that, with cotton balls process after blade 6 hours with interior without rain, or water breakthrough not.
5. method according to claim 1, is characterized in that, described weed killer herbicide is glyphosate or phosphine oxamate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210019682 CN102577857B (en) | 2012-01-24 | 2012-01-24 | Method for identifying herbicide resistance of cotton leaves by cotton ball positioning |
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| CN 201210019682 CN102577857B (en) | 2012-01-24 | 2012-01-24 | Method for identifying herbicide resistance of cotton leaves by cotton ball positioning |
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| CN102577857B true CN102577857B (en) | 2013-11-06 |
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| CN103081731B (en) * | 2013-02-28 | 2014-12-31 | 中国农业科学院棉花研究所 | Method for identifying glyphosate resistant cotton rapidly |
| CN105379570A (en) * | 2015-11-16 | 2016-03-09 | 中国农业科学院棉花研究所 | Method for rapidly identifying glufosinate-ammonium resisting cotton |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102165885A (en) * | 2010-12-15 | 2011-08-31 | 中国农业科学院植物保护研究所 | Method for identifying glyphosate resistance of cotton |
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| US6818807B2 (en) * | 2001-08-06 | 2004-11-16 | Bayer Bioscience N.V. | Herbicide tolerant cotton plants having event EE-GH1 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102165885A (en) * | 2010-12-15 | 2011-08-31 | 中国农业科学院植物保护研究所 | Method for identifying glyphosate resistance of cotton |
Non-Patent Citations (2)
| Title |
|---|
| 作物的功能叶;鄂玉江;《新农业》;19841231(第15期);第15页 * |
| 转基因抗草甘膦棉花鉴定方法研究;王秀丽等;《山东农业科学》;20081231(第09期);第79-80页 * |
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Granted publication date: 20131106 Termination date: 20180124 |