CN105379570A - Method for rapidly identifying glufosinate-ammonium resisting cotton - Google Patents
Method for rapidly identifying glufosinate-ammonium resisting cotton Download PDFInfo
- Publication number
- CN105379570A CN105379570A CN201510784240.XA CN201510784240A CN105379570A CN 105379570 A CN105379570 A CN 105379570A CN 201510784240 A CN201510784240 A CN 201510784240A CN 105379570 A CN105379570 A CN 105379570A
- Authority
- CN
- China
- Prior art keywords
- cotton
- radicle
- phosphine oxamate
- clear water
- soaked
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a method for rapidly identifying glufosinate-ammonium resisting cotton. The method includes the steps that white-showing to-be-detected cotton seeds are soaked with a glufosinate-ammonium solution; after being washed with fresh water, the soaked seeds are arranged in an illumination culturing box to be subjected to sand culturing; the number of seedlings and the length of cultured roots are counted respectively, and the emergence rate and the radicle growth inhibition ratio are calculated; whether to-be-detected cotton is the glufosinate-ammonium resisting cotton or not is judged according to the emergence rate and the radicle growth inhibition ratio, wherein the to-be-detected cotton with the emergence rate larger than or equal to 80% or the radicle growth inhibition ratio smaller than or equal to 40% is the glufosinate-ammonium resisting cotton, and the to-be-detected cotton with the emergence rate smaller than or equal to 40% or the radicle growth inhibition ratio larger than or equal to 80% is not the glufosinate-ammonium resisting cotton. The method has the advantages of being short in identifying period, small in chemical use quantity and workload, free of season limitation and the like, and bases are provided for breeding of the glufosinate-ammonium resisting cotton variety.
Description
Technical field
The present invention relates to agricultural technology field, specifically, relate to the rapid identification method of a kind of anti-phosphine oxamate cotton.
Background technology
Cotton is the important economic crops of China, long-term cultivated area 5,000,000 hm
2, accounting for 26% of the economic crops gross area, is key pillars of the national economy industries.But China's weed in cotton field harm is heavier, causes damage 14% ~ 16%, seriously constrains the high-quality of cotton, High-efficient Production throughout the year.Grass ammonium phosphine (glufosinate-ammonium) is by Hirst company (now belonging to Beyer Co., Ltd) Development and Production; chemical name is 4-[hydroxyl (methyl) phosphono]-DL-high lactamine; belong to phosphonic acid based weed killer herbicide; glutamine synthetase inhibitor, non-selective (natural disposition of going out) contact killing type weed killer herbicide.Grass ammonium phosphine was developed twentieth century eighties, was second largest genetically modified crops herbicide-tolerant in the world.The part malignant weed removal effect of grass ammonium phosphine to tolerate glyphosate is very good, and after dispenser in the short time, in plant corpus, disorder is sunk in ammonium metabolism, and cytotoxic agent ammonium ion is accumulated in plant corpus, and meanwhile, photosynthesis is seriously suppressed, and reaches weeding object.Grass ammonium phosphine toxicity is low, safer, is easy to degraded in soil, to crop safety, not easily drift, herbicidal spectrum is wide, active high, consumption is few, and environmental pressure is little, and herbicide is rapid, grass family and the broad leaved weed of more than 100 kinds can be killed fast, available water does base, safe and convenient to use, plays an important role in the preventing and kill off of weed in cotton field.But, because phosphine oxamate does not have the selectivity between weeds and cotton, when using phosphine oxamate, often easy poisoning is caused to non-Bt cotton kind, even dead.
The transgenic technology speed of development is surprising, and the tremendous contribution of genetically modified crops to agricultural is approved widely, and its plantation is in recent years also in the gesture growed in intensity.The application of the anti-phosphine oxamate cotton of transgenosis not only can avoid the generation of poisoning, can also expand the scope of application and the flexibility of phosphine oxamate.Grass ammonium phosphine is as a kind of excellent steriland herbicide, very be suitable as resistant gene, Hirst company develop actively genetically modified crops, with careless ammonium phosphine for target, carry out resistant gene transfer, seed selection resistant crop varieties, at present, the resistant gene of careless ammonium phosphine is successfully applied in 20 various crop such as cotton by Hirst company, these genetically modified crops are not only generally planted at the vast north America region based on the U.S., start in Asia in recent years, Australia, Some European country and regional popularizing planting.High dose uses careless ammonium phosphine to have certain injury to genetically modified crops, but poisoning is light, little to yield effect.Anti-careless ammonium phosphine crop safety dosage can up to 2.0kg/hm
2above, 0.5 ~ 1.0kg/hm
2careless ammonium phosphine can prevent and kill off most of weeds.
At present, China is implementing transgenosis anti-phosphine oxamate new cotton variety incubation program, has obtained the transgene cotton new material of a collection of stably express.Along with the raising of agricultural modernization level, a large amount of urbanization transfer of rural laborer, the demand of cotton producer to antiweed kind is more and more stronger.Transgenosis anti-phosphine oxamate cotton certainly will by become China be expected to after glyphosate resistant cotton promote another kind of Transgenic Resistant Herbicide Cotton Industry kind.In anti-phosphine oxamate cotton variety Breeding Process, the basis that phosphine oxamate evaluation of resistance is not only cotton breeding and Industry Promotion is carried out to cotton material, also be one of content of carrying out the evaluation of transgene cotton environmental safety, therefore, detection and the authentication method of developing a kind of anti-phosphine oxamate cotton just seem particularly important.
At present, the authentication method of anti-phosphine oxamate cotton is mainly field or greenhouse plant Resistance Identification method, namely in field or chamber planting cotton to be measured, whether after cotton grows to certain leaf age, spray certain density phosphine oxamate, be that glyphosate resistant cotton judges according to the dead degree of cotton plants after dispenser to it.The method is intuitively accurate, but length consuming time, take up room large, seasonal strong.Therefore, set up a kind of quick, easy, low cost and accurately and effectively anti-phosphine oxamate cotton authentication method for China anti-phosphine oxamate cotton popularization and detect significant.
Summary of the invention
The object of this invention is to provide the rapid identification method of a kind of anti-phosphine oxamate cotton.
In order to realize the object of the invention, the rapid identification method of a kind of anti-phosphine oxamate cotton of the present invention, described method comprises: soaked by the cotton seeds phosphine oxamate solution to be measured showed money or valuables one carries unintentionally; After clear water rinses, by the seed that soaked be placed in illumination box and carry out sand culture; Statistics is emerged and is counted and Baconic's length respectively, calculates emergence rate and radicle growth inhibiting rate; Judge whether cotton to be measured is anti-phosphine oxamate cotton according to emergence rate and radicle growth inhibiting rate, wherein emergence rate >=80% or radicle growth inhibiting rate≤40% is glyphosate resistant cotton, and emergence rate≤40% or radicle growth inhibiting rate >=80% is non-anti-phosphine oxamate cotton.
Aforesaid method, cotton seeds to be measured carries out the process of sulfuric acid lint in advance, then seed is soaked 24 hours in clear water, obtains the seed showed money or valuables one carries unintentionally.
Aforesaid method, soaks the phosphine oxamate solution of the cotton seeds active ingredient concentration to be measured 0.5 ~ 2.0g/L showed money or valuables one carries unintentionally 0.5 ~ 2 hour, and rinses subsequently, i.e. phosphine oxamate processed group with water; Meanwhile, if clear water control treatment group, namely sample to be tested is soaked with clear water.
Aforesaid method, the concrete operations of sand culture are: being sieved by fine sand is placed in drying box, 180 DEG C of hot air sterilizations 4 hours, dry in the air cool after fine sand and water are mixed according to the weight ratio of 25:4, loading specification is in the germination box of 13cm × 19cm × 12cm, to about 2/3 height; The cotton seeds soaked evenly is put in germination box, about 50 seed/boxes, repeat 4 times; Then on seed, cover the thick loosen loose sand of about 1.5cm, cover lid is placed in illumination box and cultivates.
Wherein, the temperature of illumination box is set as 30 DEG C, humidity 60% ~ 80%, and illumination 8 hours/dark 16 hours, intensity of illumination is 6000lx.
Sand culture, after 7 days, adds up the number of emerging of the cotton seeds of clear water control treatment group and phosphine oxamate processed group respectively, calculates emergence rate, and statistics radicle is long, and calculates radicle growth inhibiting rate; Wherein the criterion of seed sprouting is that cotyledon breaks ground.The computing formula of radicle growth inhibiting rate is:
The radicle of radicle growth inhibiting rate %=(radicle of the radicle length-glyphosate processed group of clear water control treatment group is long) × 100/ clear water control treatment group is long
In the rapid identification method of above-mentioned anti-phosphine oxamate cotton, in described statistics and calculation procedure, go back observable radicle growth situation; In described determining step, also can judge whether cotton to be measured is anti-phosphine oxamate cotton, and wherein radicle growth is anti-phosphine oxamate cotton normally according to radicle growth situation, and radicle growth is suppressed, be non-anti-phosphine oxamate cotton without side root.
The present invention has the following advantages:
(1) cycle is short.Within general about 8 days, qualification result can be obtained.Compared with field or greenhouse Resistance Identification method, shorten about 10 days qualification cycle.
(2) dosage is few.Soak seed with a small amount of medicament just can identify, compared with field or greenhouse Resistance Identification method, save a large amount of dosage of spraying, authentication method of the present invention can repeat, and result uniformity is better.
(3) workload is little.Authentication method of the present invention completes in indoor, and without the need to carrying out the operations such as whole, sowing, dispenser, a people just can complete whole qualification process.And it is little to take up room, can identify multiple material simultaneously.
(4) be not subject to seasonal restrictions.Authentication method of the present invention is at in-house operation, and seed is cultivated in illumination box.Compared with field or greenhouse Resistance Identification method, authentication method of the present invention avoids the impact of bad climate.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art, is raw materials usedly commercial goods.
The test material used in following examples: anti-phosphine oxamate cotton material totally 6 kinds, is respectively ZYA1, ZYA2, ZYA3, ZYA4, ZYA5, ZYA6; Non-anti-phosphine oxamate cotton material totally 3 kinds, is respectively CCRI 52, CCRI 65, CCRI 83.Above-mentioned material provides by the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute's breeding chamber.Phosphine oxamate used is the blue or green frost of purple electricity, and it is the phosphine oxamate aqua of concentration 200g/L, and effluent north Shijiazhuang City Long Hui fine chemistry industry Co., Ltd produces.Quantitative test in embodiment, all arranges and repeats experiment for 4 times, results averaged.
The rapid identification method of the anti-phosphine oxamate cotton of embodiment 1
Test method:
1, vernalization
All cotton seeds are carried out sulfuric acid lint, and photon process is for subsequent use.Then the seed getting often kind of detected materials soaks 24 hours in clear water, gets the seed showed money or valuables one carries unintentionally for subsequent use.
2, soak
Each sample to be tested selects that vernalization shows money or valuables one carries unintentionally, full cotton seeds, soaks, rinse subsequently with water with phosphine oxamate solution.Meanwhile, if clear water control treatment group: soak sample to be tested with clear water.
3, sand culture
Being sieved by fine sand is placed in drying box, 180 DEG C of hot air sterilizations 4 hours, dry in the air cool after fine sand and water are mixed according to the weight ratio of 25:4, loading specification is in the germination box of 13cm × 19cm × 12cm, to about 2/3 height; The cotton seeds soaked evenly is put in germination box, about 50 seed/boxes, repeat 4 times; Then on seed, cover the thick loosen loose sand of about 1.5cm, cover lid is placed in illumination box and cultivates.
The temperature of illumination box is set as 30 DEG C, humidity 60% ~ 80%, and illumination 8 hours/dark 16 hours, intensity of illumination is 6000lx.
4, statistics and calculating
Sand culture, after 7 days, adds up the number of emerging of the cotton seeds of clear water control treatment group and phosphine oxamate processed group respectively, calculates emergence rate, and statistics radicle is long, and calculates radicle growth inhibiting rate; Wherein the criterion of seed sprouting is that cotyledon breaks ground.The computing formula of radicle growth inhibiting rate is:
The radicle of radicle growth inhibiting rate %=(radicle of the radicle length-glyphosate processed group of clear water control treatment group is long) × 100/ clear water control treatment group is long
According to the method described above, select anti-phosphine oxamate cotton material ZYA1, ZYA2, ZYA3 and non-anti-phosphine oxamate cotton material CCRI 52, CCRI 65 as cotton material to be identified.Prepare the phosphine oxamate solution that active ingredient concentration is 0.5g/L, 1.0g/L, 2.0g./L respectively, establish clear water control group to do for subsequent use simultaneously.In different soak time (0.5 hour, 1 hour, 2 hours), the cotton seeds to be measured showed money or valuables one carries unintentionally is soaked with the phosphine oxamate solution prepared and clear water respectively, after sand culture, carry out the judgement of anti-phosphine oxamate cotton.Experimental result is in table 1.
The emergence rate of table 1 cotton seeds to be measured after the phosphine oxamate process of variable concentrations and radicle growth inhibiting rate
§: radicle growth inhibiting rate is that negative value represents that the radicle of phosphine oxamate processed group is grown up in clear water control group, illustrates that phosphine oxamate process has facilitation to radicle growth.
As shown in Table 1, cotton seed to be measured is soaked after 0.5 hour with the phosphine oxamate solution that active ingredient concentration is 0.5 ~ 2.0g/L, the emergence rate of 3 kinds of anti-phosphine oxamate cottons is 89.0% ~ 94.2%, contrast difference compared with (93.5% ~ 98.0%) with clear water little, radicle growth inhibiting rate is not all higher than 4.8%; And the emergence rate of two kinds of non-anti-phosphine oxamate cottons is only 35.9% ~ 72.3%, contrast difference compared with (91.3% ~ 92.3%) with clear water large, radicle growth inhibiting rate is 63.2 ~ 83.2%.
Cotton seed to be measured is soaked after 1.0 hours with the phosphine oxamate solution that active ingredient concentration is 0.5 ~ 2.0g/L, the emergence rate of 3 kinds of anti-phosphine oxamate cottons is 76.0% ~ 98.7%, contrast difference compared with (89.2% ~ 92.6%) with clear water little, radicle growth inhibiting rate is not all higher than 15.0%; And the emergence rate of two kinds of non-anti-phosphine oxamate cottons is only 23.6% ~ 42.3%, contrast difference compared with (93.1% ~ 95.4%) with clear water large, radicle growth inhibiting rate is 78.5 ~ 87.4%.
Cotton seed to be measured is soaked after 2.0 hours with the phosphine oxamate solution that active ingredient concentration is 0.5 ~ 2.0g/L, the emergence rate of 3 kinds of anti-phosphine oxamate cottons is 75.5% ~ 95.3%, contrast difference compared with (84.8% ~ 91.8%) with clear water little, radicle growth inhibiting rate is not all higher than 38.5%; And the emergence rate of two kinds of non-anti-phosphine oxamate cottons is only 0% ~ 18.4%, contrast difference compared with (88.7% ~ 96.3%) with clear water large, radicle growth inhibiting rate is up to 85.1 ~ 91.3%.
As can be seen here, be in the phosphine oxamate solution of 0.5 ~ 2.0g/L in active ingredient concentration, along with soak time increases, the impact of antagonism phosphine oxamate cotton is little, very large on the impact of non-anti-phosphine oxamate cotton.The phosphine oxamate processing method antagonism phosphine oxamate cotton that the present invention adopts and the impact of non-anti-phosphine oxamate cotton have obvious difference, and therefore, discrimination method of the present invention can distinguish anti-phosphine oxamate cotton and non-anti-phosphine oxamate cotton effectively.
The rapid identification method of the anti-phosphine oxamate cotton of embodiment 2
According to the method for embodiment 1, select anti-phosphine oxamate cotton material (ZYA4, ZYA5, ZYA6) and non-anti-phosphine oxamate cotton material (CCRI 52, CCRI 65, CCRI 83) as cotton material to be identified, by the phosphine oxamate solution immersion treatment 1 hour that active ingredient concentration is 1.0g/L, experimental result is in table 2.
Table 2 processes the long and radicle growth inhibiting rate of the emergence rate of rear cotton seeds to be measured, radicle
As can be seen from Table 2, for anti-phosphine oxamate cotton material, phosphine oxamate process is on emergence rate impact little (emergence rate is down to 89.7% ~ 92.3% from 91.7% ~ 98.2%), and radicle growth inhibiting rate is only less than 5%, also has promote radicle growth effect to some material.For non-anti-phosphine oxamate cotton material, phosphine oxamate process significantly affects emergence rate (emergence rate is down to 8.7% ~ 16.2% from 91.1% ~ 98.5%), and radicle growth inhibiting rate is up to 79.2% ~ 90.2%; As can be seen here, the impact of phosphine oxamate process on non-anti-phosphine oxamate cotton and anti-phosphine oxamate cotton that the present invention uses has remarkable difference, and therefore, discrimination method of the present invention can distinguish anti-phosphine oxamate cotton and anti-non-phosphine oxamate cotton effectively.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (7)
1. a rapid identification method for anti-phosphine oxamate cotton, is characterized in that, described method comprises: soaked by the cotton seeds phosphine oxamate solution to be measured showed money or valuables one carries unintentionally; After clear water rinses, by the seed that soaked be placed in illumination box and carry out sand culture; Statistics is emerged and is counted and Baconic's length respectively, calculates emergence rate and radicle growth inhibiting rate; Judge whether cotton to be measured is anti-phosphine oxamate cotton according to emergence rate and radicle growth inhibiting rate, wherein emergence rate >=80% or radicle growth inhibiting rate≤40% is glyphosate resistant cotton, and emergence rate≤40% or radicle growth inhibiting rate >=80% is non-anti-phosphine oxamate cotton.
2. method according to claim 1, is characterized in that, cotton seeds to be measured carries out the process of sulfuric acid lint in advance, then seed is soaked 24 hours in clear water, obtains the seed showed money or valuables one carries unintentionally.
3. method according to claim 2, is characterized in that, is soaked 0.5 ~ 2 hour by the phosphine oxamate solution of the cotton seeds active ingredient concentration to be measured 0.5 ~ 2.0g/L showed money or valuables one carries unintentionally, and rinses subsequently, i.e. phosphine oxamate processed group with water; Meanwhile, if clear water control treatment group, namely sample to be tested is soaked with clear water.
4. method according to claim 3, it is characterized in that, the concrete operations of sand culture are: being sieved by fine sand is placed in drying box, 180 DEG C of hot air sterilizations 4 hours, dry in the air cool after fine sand and water are mixed according to the weight ratio of 25:4, loading specification is in the germination box of 13cm × 19cm × 12cm, to 2/3 height; The cotton seeds soaked evenly is put in germination box, 50 seed/boxes, repeat 4 times; Then on seed, cover the thick loosen loose sand of 1.5cm, cover lid is placed in illumination box and cultivates.
5. method according to claim 4, is characterized in that, the temperature of illumination box is set as 30 DEG C, humidity 60% ~ 80%, and illumination 8 hours/dark 16 hours, intensity of illumination is 6000lx.
6. method according to claim 5, is characterized in that, sand culture, after 7 days, adds up the number of emerging of the cotton seeds of clear water control treatment group and phosphine oxamate processed group respectively, calculates emergence rate, and statistics radicle is long, and calculates radicle growth inhibiting rate; Wherein the criterion of seed sprouting is that cotyledon breaks ground.
7. the method according to any one of claim 1-6, is characterized in that, the computing formula of radicle growth inhibiting rate is:
The radicle of radicle growth inhibiting rate %=(radicle of the radicle length-glyphosate processed group of clear water control treatment group is long) × 100/ clear water control treatment group is long.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510784240.XA CN105379570A (en) | 2015-11-16 | 2015-11-16 | Method for rapidly identifying glufosinate-ammonium resisting cotton |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510784240.XA CN105379570A (en) | 2015-11-16 | 2015-11-16 | Method for rapidly identifying glufosinate-ammonium resisting cotton |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105379570A true CN105379570A (en) | 2016-03-09 |
Family
ID=55412833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510784240.XA Pending CN105379570A (en) | 2015-11-16 | 2015-11-16 | Method for rapidly identifying glufosinate-ammonium resisting cotton |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105379570A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114586496A (en) * | 2022-02-17 | 2022-06-07 | 江西省棉花研究所 | Identification method for glyphosate resistance of cotton seeds |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577857A (en) * | 2012-01-24 | 2012-07-18 | 中国农业科学院棉花研究所 | Method for identifying herbicide resistance of cotton leaves by cotton ball positioning |
| CN103081731A (en) * | 2013-02-28 | 2013-05-08 | 中国农业科学院棉花研究所 | Method for identifying glyphosate resistant cotton rapidly |
| CN104186308A (en) * | 2014-08-28 | 2014-12-10 | 兰州大学 | Culture medium for anti-glufosinate-ammonium alfalfa plants and soil screening method |
-
2015
- 2015-11-16 CN CN201510784240.XA patent/CN105379570A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577857A (en) * | 2012-01-24 | 2012-07-18 | 中国农业科学院棉花研究所 | Method for identifying herbicide resistance of cotton leaves by cotton ball positioning |
| CN103081731A (en) * | 2013-02-28 | 2013-05-08 | 中国农业科学院棉花研究所 | Method for identifying glyphosate resistant cotton rapidly |
| CN104186308A (en) * | 2014-08-28 | 2014-12-10 | 兰州大学 | Culture medium for anti-glufosinate-ammonium alfalfa plants and soil screening method |
Non-Patent Citations (3)
| Title |
|---|
| 冯艳等: "草铵膦对棉花根系发育影响及其生理机制", 《中国农学通报》 * |
| 孙磊等: "大豆再生植株抗草铵膦的筛选方法研究", 《大豆科学》 * |
| 陈旭升: "抗除草剂棉花研究进展", 《江西农业学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114586496A (en) * | 2022-02-17 | 2022-06-07 | 江西省棉花研究所 | Identification method for glyphosate resistance of cotton seeds |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Polania et al. | Estimation of phenotypic variability in symbiotic nitrogen fixation ability of common bean under drought stress using 15N natural abundance in grain | |
| WU et al. | Goosegrass (Eleusine indica) density effects on cotton (Gossypium hirsutum) | |
| Khayamim et al. | Seed germination, plant establishment, and yield of sugar beet genotypes under salinity stress. | |
| CN101897253A (en) | Method for identifying seedling stage stress resistance of tobacco variety | |
| Passam et al. | Influence of harvest time and after-ripening on the seed quality of eggplant | |
| CN105349684A (en) | Molecular marker closely linked to maize rough dwarf disease resistant main effect QTL | |
| CN104920089A (en) | Screening method for flood-resisting parent for breeding direct-sowing rice | |
| CN105359855B (en) | Improved time of infertility Artificial disease nursery cotton variety resistance to verticillium wilt identification method | |
| CN103081731B (en) | Method for identifying glyphosate resistant cotton rapidly | |
| CN101990821B (en) | Method for identifying moisture resistance of sesame during germination | |
| Cruz et al. | Assessment of phenotypic diversity in the USDA, National Plant Germplasm System (NPGS) guayule germplasm collection | |
| CN106048056A (en) | Wheat seed dormancy persistence-substantially related SNP label and its CAPS label and use | |
| CN105379570A (en) | Method for rapidly identifying glufosinate-ammonium resisting cotton | |
| CN105123301B (en) | A kind of Forecasting Methodology of soil tobacco black shank morbidity potentiality | |
| Rehman et al. | Breeding potential of upland cotton for water stress tolerance. | |
| CN107750509A (en) | Barley moisture-proof simple Identification method | |
| Hu et al. | QTL analysis of nitrogen content of plant shoot under two nitrogen conditions in rice ('Oryza sativa'L.) | |
| CN102766625A (en) | Molecular marker of rice major gene bph22 (t) resistant to brown planthoppers and application thereof | |
| CN110476744A (en) | Floral leaf character is increasing the application on rape group child's hair twisted in a knot-childhood pericarp area | |
| CN102269703B (en) | Method for detecting glyphosate resistance of plant | |
| Zhou et al. | Cotton yield stability achieved through manipulation of vegetative branching and photoassimilate partitioning under reduced seedling density and double seedlings per hole | |
| CN104542026A (en) | Method for screening or identifying herbicide-resistant rice via agarose matrix | |
| Milatović et al. | Evaluation of late plum cultivars in the region of Belgrade (Serbia) | |
| CN106718828A (en) | A kind of breeding method of drought-enduring type rice varieties | |
| CN106034659A (en) | Method for rapidly identifying winterness and springness of wheat indoors |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160309 |
|
| RJ01 | Rejection of invention patent application after publication |