CN104366037A - Aquatic product liver and gallbladder syndrome control feed additive and preparation method thereof - Google Patents
Aquatic product liver and gallbladder syndrome control feed additive and preparation method thereof Download PDFInfo
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- CN104366037A CN104366037A CN201410020812.2A CN201410020812A CN104366037A CN 104366037 A CN104366037 A CN 104366037A CN 201410020812 A CN201410020812 A CN 201410020812A CN 104366037 A CN104366037 A CN 104366037A
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Abstract
The invention discloses an aquatic product liver and gallbladder syndrome control feed additive comprising the following components by weight: 10-20 parts of rhubarb, 5-25 parts of rhizoma coptidis, 15-30 parts of plantain, 8-15 parts of herba artemisiae scopariae, 10-30 parts of honeysuckle, 2-10 parts of licorice, 1-10 parts of vitamin C and 1-10 parts of vitamin B6. The invention also discloses a preparation method of the feed additive, through microbial fermentation method, the herbal components can fully play the treatment effect under the action of microorganisms, and the feed additive can effectively prevent and cure aquatic product liver and gallbladder syndrome.
Description
Technical field
The present invention relates to the technical field of feed addictive, particularly relate to feed addictive of a kind of water prevention product liver and gall syndrome and preparation method thereof.
Background technology
In the last few years, the aquaculture industry of China experienced by and developed rapidly, and achieved very large achievement, and only over 20 years, China's aquatic products output of production is sure to occupy the first in the world.Meanwhile, the appearance of high density, intensive culture pattern and development, cause breeding environment to worsen, fish disease break out with popular, thus having there is the new fish disease taking hepatobiliary disease as principal character, i.e. liver and gall syndrome of fish, brings larger negative effect to aquaculture.
Liver and gall syndrome is nearly 2 years one of fish diseases very frequently in fish disease occurs, epidemic season mainly has generation up to October from June, generally popular in all parts of the country, especially fry, the fingerling incidence of disease are high, harm object mainly grass carp, crucian, perch, large yellow croaker, snakehead, sparid class, carp, channel catfish, Yun Ban Channel-catfish, rainbow trout, schizothoracin, megalobrama amblycephala, black carp, Tilapia mossambica, be also common in soft-shelled turtle.Because general mistaken diagnosis is other fish disease, medication is not suited the medicine to the illness, and the death rate can reach 50%-60%, even can reach 60%-90%.
Liver and gall syndrome of fish, be estimate liver and gall enlargement, variable color is classical symptom.Sick fish early stage, liver is enlargement slightly, slight anaemia, and color is partially light; With regard to gall-bladder, there will be look comparatively dark, show slightly green.Along with PD, the obvious enlargement of liver, comparable large more than 1 times under normal circumstances, Hepatic turns yellow gradually and turns white, or yellow red and white is alternate in patch shape, forms significantly " flower liver " symptom, and make liver local or the major part that have become " green liver "; Some livers touch frangible, and the obvious enlargement 1-2 of gall-bladder doubly (causes bile to overflow or rupture of gallbladder) sometimes, and bile darkens green or blackish green, or bleaches until colourless, and the hyperemia of severe one gall-bladder is rubescent, and make bile also become red.Sometimes, the spleen kidney also obviously enlargement of sick fish, organ systems expands simultaneously.Because serious change, impaired appears in main organs, the resistance against diseases of body declines, and to the intrusion of other germ with opportunity, therefore this seriously ill disease person is normal simultaneously with the disease such as hemorrhage, gill rot, enteritis, rotten tail.This sick most typical feature is liver and gall enlargement and variable color.During diagnosis, outside inspection fish body surface and the pathology such as the gill, eye, fish belly should be cut open, conscientiously check liver and gall pathology situation, if liver, courage enlargement and variable color are obvious, tentatively can be defined as liver and gall syndrome.
The reason of liver and gall syndrome is caused to mainly contain four large classes: one, cause pathogeny imcrobe infection.Cultivation density is excessive, water body environment worsens, and the cause pathogeny imcrobe infections such as the Aeromonas hydrophila in water body, tarda and reovirus, all directly or indirectly can cause fish generation hepatitis.Pathogenic microorganism is amount reproduction in fish liver, produces toxin, causes liver cell dysbolism, cause degeneration of liver cells, necrosis; Two, Drug factor.As heavy metal poisoning, agricultural chemicals, ammonia nitrogen, nitrite, hydrogen sulfide poisoning etc., can cause the sex change of fish hepatic tissue, necrosis.Adding the prejudicial medicine of fish liver and inappropriate medication for a long time in feed is the main cause that hepatitis due to medicine occurs.Low dosage, in feed, add furazolidone, chloramphenicol, sulfamido etc. for a long time, or employ large, the residual high fish medicine of side effect as the medicine such as metrifonate, copper sulphate, cause hepatic injury; Three, be feed become sour rotten, nutritional labeling unbalance and feed in containing noxious material and hypovitaminosis; Four, in feed, carbohydrate content is higher causes.Carbohydrate content height can cause fish carbohydrate metabolism disturbance, causes interior liver Fat Accumulation, destroys liver function, the normal physiological metabolism of interference fish.
In the prior art, the method for the treatment of aquatic products liver and gall syndrome generally adopts simple Chinese herbal medicine to combine, although can obtain certain curative effect, result for the treatment of is not good.
In view of this, the present inventor's research and the feed addictive devising a kind of water prevention product liver and gall syndrome and preparation method thereof, this case produces thus.
Summary of the invention
The object of the present invention is to provide a kind of feed addictive of water prevention product liver and gall syndrome, use this additive effectively can play the drug effect of Chinese herbal medicine, good to aquatic products liver and gall syndrome result for the treatment of.
Another object of the present invention is to provide a kind of preparation method of feed addictive of water prevention product liver and gall syndrome, by microbe fermentation method, the drug effect of Chinese herbal medicine is given full play of, to prepare described feed addictive.
The technical scheme adopted that the present invention solves its technical problem is:
A feed addictive for water prevention product liver and gall syndrome, comprises the component of following parts by weight: 10-20 part rheum officinale, 5-25 part coptis, 15-30 part Asiatic plantain, 8-15 part oriental wormwood, 10-30 part honeysuckle, 2-10 part Radix Glycyrrhizae, 1-10 part vitamin C and 1-10 part Cobastab
6.
As the preferred embodiment of embodiment, the weight proportion of said components is preferably: 10-15 part rheum officinale, 10-15 part coptis, 15-20 part Asiatic plantain, 8-10 part oriental wormwood, 10-15 part honeysuckle, 5-10 part Radix Glycyrrhizae, 2-5 part vitamin C and 2-5 part Cobastab
6.
As the preferred embodiment of embodiment, the weight proportion of said components is preferably: 12 parts of rheum officinales, 12 portions of coptiss, 18 portions of Asiatic plantains, 8 portions of oriental wormwoods, 12 portions of honeysuckles, 8 parts of Radix Glycyrrhizaes, 3 parts of vitamin Cs and 3 parts of Cobastabs
6.
A preparation method for the feed addictive of water prevention product liver and gall syndrome, comprises the following steps:
Step one, take each component according to above-mentioned weight proportion, and each component is ground, obtain 10-20 order meal;
Step 2, soak above-mentioned rheum officinale, the coptis, Asiatic plantain, oriental wormwood, honeysuckle and Radix Glycyrrhizae meal with distilled water after, 120 DEG C of sterilizing 0.3h;
Step 3, sterilizing is good after the above-mentioned meal obtained by step 2 be seeded in Bacillus acidi lactici liquid, fermentation 2-3 days;
After step 4, fermentation ends, dry zymotic fluid, obtained dry powder;
Step 5, the vitamin C that step one is taken and Cobastab
6be added in above-mentioned dry powder, be this feed addictive.
As the preferred embodiment of embodiment, the preparation process of described Bacillus acidi lactici liquid is: take 10 g peptones, 10 g beef extracts, 5 g yeast extracts, 2 g diammonium hydrogen citrate ((NH
4)
2hC
6h
5o
7), 20g glucose (C
6h
12o
6h
2o), 1 mL Tween 80,5g sodium acetate (CH
3cOONa3H
2o), 2g dipotassium hydrogen phosphate (K
2hPO
43H
2o), 0.58 g magnesium sulfate (MgSO
47H
2and 0.25 g manganese sulfate (MnSO O)
4h
2o), distilled water constant volume is to 1 L, and 120 DEG C of autoclaving 15 min, adjust pH to 6.2 ~ 6.6, obtained fluid nutrient medium; Be inoculated in by Bacillus acidi lactici on fluid nutrient medium, cultivation temperature is 30 DEG C, and shaking speed is 160r/min, cultivates 24h, i.e. obtained this Bacillus acidi lactici liquid.
As the preferred embodiment of embodiment, in step 3, the concentration of Bacillus acidi lactici is 5,000,000,000 cfu/g.
Adopt herbal component prepared by the inventive method, on the one hand, give full play to the drug effect of each component of Chinese herbal medicine, on the other hand, by the fermentation of microorganism, Chinese herbal medicine wherein some component is used by microorganism, microorganism passes through metabolism, produce metabolite and secondary metabolite, advantageously in the absorption of aquatic products to this type of material, and then the recovery of aquatic products liver and gall syndrome can be impelled.
Detailed description of the invention
embodiment 1
A feed addictive for water prevention product liver and gall syndrome, comprises the component of following parts by weight: 10 rheum officinales, 5 portions of coptiss, 15 portions of Asiatic plantains, 8 portions of oriental wormwoods, 10 portions of honeysuckles, 2 parts of Radix Glycyrrhizaes, 1 part of vitamin C and 1 part of Cobastab
6.
embodiment 2
A feed addictive for water prevention product liver and gall syndrome, comprises the component of following parts by weight: 20 parts of rheum officinales, 25 portions of coptiss, 30 portions of Asiatic plantains, 15 portions of oriental wormwoods, 30 portions of honeysuckles, 10 parts of Radix Glycyrrhizaes, 10 parts of vitamin Cs and 10 parts of Cobastabs
6.
embodiment 3
A feed addictive for water prevention product liver and gall syndrome, comprises the component of following parts by weight: 12 parts of rheum officinales, 12 portions of coptiss, 18 portions of Asiatic plantains, 8 portions of oriental wormwoods, 12 portions of honeysuckles, 8 parts of Radix Glycyrrhizaes, 3 parts of vitamin Cs and 3 parts of Cobastabs
6.
embodiment 4
A preparation method for the feed addictive of water prevention product liver and gall syndrome, comprises the following steps:
Step one, take each component according to above-mentioned weight proportion, and each component is ground, obtain 10-20 order meal;
Step 2, soak above-mentioned rheum officinale, the coptis, Asiatic plantain, oriental wormwood, honeysuckle and Radix Glycyrrhizae meal with distilled water after, 120 DEG C of sterilizing 0.3h;
Step 3, sterilizing is good after the above-mentioned meal obtained by step 2 be seeded in Bacillus acidi lactici liquid, fermentation 2-3 days;
After step 4, fermentation ends, dry zymotic fluid, obtained dry powder;
Step 5, the vitamin C that step one is taken and Cobastab
6be added in above-mentioned dry powder, be this feed addictive.
As the preferred embodiment of embodiment, the preparation process of described Bacillus acidi lactici liquid is: take 10 g peptones, 10 g beef extracts, 5 g yeast extracts, 2 g diammonium hydrogen citrate ((NH
4)
2hC
6h
5o
7), 20g glucose (C
6h
12o
6h
2o), 1mL Tween 80,5g sodium acetate (CH
3cOONa3H
2o), 2g dipotassium hydrogen phosphate (K
2hPO
43H
2o), 0.58 g magnesium sulfate (MgSO
47H
2and 0.25 g manganese sulfate (MnSO O)
4h
2o), distilled water constant volume is to 1 L, and 120 DEG C of autoclaving 15 min, adjust pH to 6.2 ~ 6.6, obtained fluid nutrient medium; Be inoculated in by Bacillus acidi lactici on fluid nutrient medium, cultivation temperature is 30 DEG C, and shaking speed is 160r/min, cultivates 24h, i.e. obtained this Bacillus acidi lactici liquid.
As the preferred embodiment of embodiment, in step 3, the concentration of Bacillus acidi lactici is 5,000,000,000 cfu/g.
To the grass carp phenomena of mortality one by one that certain reservoir occurs, the grass carp of the present inventor to death is dissected, and finds its liver enlargement, slight anaemia, and look slightly light; Gall-bladder look darker, aobvious green, diagnosablely suffered from liver and gall syndrome for grass carp, by using the feed addictive of the present embodiment 1-3, mixed feed is rendered in reservoir, after treating 1 week, every day throws in once, and symptom is obviously alleviated, and cuts open inspection and finds that liver and gall recover normal substantially, food recovers normal, and the phenomena of mortality stop.In the later stage, by grouping experiment, find in described embodiment 1-3, especially with the result for the treatment of of embodiment 3 for the best.
Adopt herbal component prepared by the inventive method, on the one hand, give full play to the drug effect of each component of Chinese herbal medicine, on the other hand, by the fermentation of microorganism, Chinese herbal medicine wherein some component is used by microorganism, microorganism passes through metabolism, produce metabolite and secondary metabolite, advantageously in the absorption of aquatic products to this type of material, and then the recovery of aquatic products liver and gall syndrome can be impelled.
The above, be only present pre-ferred embodiments, therefore can not limit scope of the invention process successively, the equivalence change namely done according to the scope of the claims of the present invention and description with modify, all should still belong in scope that the present invention contains.
Claims (6)
1. a feed addictive for water prevention product liver and gall syndrome, is characterized in that: the component comprising following parts by weight: 10-20 part rheum officinale, 5-25 part coptis, 15-30 part Asiatic plantain, 8-15 part oriental wormwood, 10-30 part honeysuckle, 2-10 part Radix Glycyrrhizae, 1-10 part vitamin C and 1-10 part Cobastab
6.
2. the feed addictive of a kind of water prevention product liver and gall syndrome as claimed in claim 1, is characterized in that: the weight proportion of said components is preferably: 10-15 part rheum officinale, 10-15 part coptis, 15-20 part Asiatic plantain, 8-10 part oriental wormwood, 10-15 part honeysuckle, 5-10 part Radix Glycyrrhizae, 2-5 part vitamin C and 2-5 part Cobastab
6.
3. the feed addictive of a kind of water prevention product liver and gall syndrome as claimed in claim 1, is characterized in that: the weight proportion of said components is preferably: 12 parts of rheum officinales, 12 portions of coptiss, 18 portions of Asiatic plantains, 8 portions of oriental wormwoods, 12 portions of honeysuckles, 8 parts of Radix Glycyrrhizaes, 3 parts of vitamin Cs and 3 parts of Cobastabs
6.
4. a preparation method for the feed addictive of water prevention product liver and gall syndrome, is characterized in that: comprise the following steps:
Step one, take each component according to such as claim 1 weight proportion, and each component is ground, obtain 10-20 order meal;
Step 2, soak above-mentioned rheum officinale, the coptis, Asiatic plantain, oriental wormwood, honeysuckle and Radix Glycyrrhizae meal with distilled water after, 120 DEG C of sterilizing 0.3h;
Step 3, sterilizing is good after the above-mentioned meal inoculating lactobacillus liquid obtained by step 2, fermentation 2-3 days;
After step 4, fermentation ends, dry zymotic fluid, obtained dry powder;
Step 5, the vitamin C that step one is taken and Cobastab
6be added in above-mentioned dry powder, be this feed addictive.
5. the preparation method of the feed addictive of a kind of water prevention product liver and gall syndrome as claimed in claim 4, is characterized in that: the preparation process of described Bacillus acidi lactici liquid is: take 10 g peptones, 10 g beef extracts, 5 g yeast extracts, 2 g diammonium hydrogen citrate ((NH
4)
2hC
6h
5o
7), 20g glucose (C
6h
12o
6h
2o), 1 mL Tween 80,5g sodium acetate (CH
3cOONa3H
2o), 2g dipotassium hydrogen phosphate (K
2hPO
43H
2o), 0.58 g magnesium sulfate (MgSO
47H
2and 0.25 g manganese sulfate (MnSO O)
4h
2o), distilled water constant volume is to 1 L, and 120 DEG C of autoclaving 15 min, adjust pH to 6.2 ~ 6.6, obtained fluid nutrient medium; Be inoculated in by Bacillus acidi lactici on fluid nutrient medium, cultivation temperature is 30 DEG C, and shaking speed is 160r/min, cultivates 24h, i.e. obtained this Bacillus acidi lactici liquid.
6. the preparation method of the feed addictive of a kind of water prevention product liver and gall syndrome as claimed in claim 4, is characterized in that: in step 3, the concentration of Bacillus acidi lactici is 5,000,000,000 cfu/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410020812.2A CN104366037A (en) | 2014-01-17 | 2014-01-17 | Aquatic product liver and gallbladder syndrome control feed additive and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410020812.2A CN104366037A (en) | 2014-01-17 | 2014-01-17 | Aquatic product liver and gallbladder syndrome control feed additive and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN104366037A true CN104366037A (en) | 2015-02-25 |
Family
ID=52545428
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410020812.2A Pending CN104366037A (en) | 2014-01-17 | 2014-01-17 | Aquatic product liver and gallbladder syndrome control feed additive and preparation method thereof |
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Cited By (5)
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| CN104770567A (en) * | 2015-04-13 | 2015-07-15 | 广西南宁桂知科技有限公司 | Feed for preventing and treating wattle-necked softshell turtle hepatitis disease and a preparation method thereof |
| CN106071391A (en) * | 2016-06-17 | 2016-11-09 | 合肥丰瑞隆生物科技有限公司 | Feed additive of cultivation Mugil cephalus and preparation method thereof |
| CN106962692A (en) * | 2017-05-16 | 2017-07-21 | 清远海贝生物技术有限公司 | A kind of premix for repairing the green liver pathological changes of grass carp and its preparation method and application |
| CN107410758A (en) * | 2017-05-16 | 2017-12-01 | 清远海贝生物技术有限公司 | A kind of premix for repairing grass carp Huang liver pathological changes and its preparation method and application |
| CN112640989A (en) * | 2020-09-08 | 2021-04-13 | 遵义蒙鸿生态农业发展有限公司 | Preparation and application of compound feed additive for enhancing immunity and promoting growth of weever |
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Application publication date: 20150225 |
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