CN104365580B - Store method and the device of animal body/plant tissue, cell, organ and body fluid - Google Patents
Store method and the device of animal body/plant tissue, cell, organ and body fluid Download PDFInfo
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- CN104365580B CN104365580B CN201410599448.XA CN201410599448A CN104365580B CN 104365580 B CN104365580 B CN 104365580B CN 201410599448 A CN201410599448 A CN 201410599448A CN 104365580 B CN104365580 B CN 104365580B
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Abstract
A kind of animal body/plant tissue, cell, the store method of organ and body fluid and device, the present invention is by the animal body/plant tissue after disinfecting, cell and organ are placed in same save set after disinfecting, afterwards this save set is placed in to freeze drier and carries out freeze drying, save set after freeze drying vacuumizes processing, under the state of save set maintenance vacuum, the drain hole place of save set is carried out to hot-melting sealed wire drawing removing is carried out in appearance body drain hole upper end and sealing-plug, holding body becomes the entirety of sealing, then preserve for a long time holding body, the present invention carries out animal body/plant soma, tissue and organ encapsulation process after freeze drying, animal body/plant soma, tissue and organ and outside air are isolated and are convenient to preserve under normal temperature state, greatly reduce preservation condition and the retain costs of animal body/plant soma, tissue and organ, to the promotion and application of the technology condition of providing convenience.
Description
Technical field
The present invention relates to the preservation field of animal and plant body tissue and organ thereof and body fluid, particularly relate to store method and the device of a kind of animal body/plant tissue, cell, organ and body fluid.
Background technology
At biology, in medical science and field of food, organic preservation is the problem that people pay close attention to always, particularly in clinical medicine and biological field, animal body/plant tissue, cell, organ and body fluid often need to preserve for a long time and keep biologically active, in process for anti-bacteria and biology enzyme biochemical reaction extend the holding time, conventionally adopt ultralow temperature Techniques of preserving, but organism, tissue and organ very easily lower the temperature and rewarming process in damaged and loss of biological activity, very high to the requirement of technology, and lower the temperature complete preservation and rewarming process are also a major challenge of technology, the process of particularly preserving, because organic matter may need to preserve the longer time, keep the just uninterrupted low-temperature condition that keeps organism of needs of its biologically active, so just have higher requirement with the energy supply of preserving ground to preserving, corresponding its retain costs can increase gradually along with the growth of holding time, the promotion and application of this technology have been caused to certain puzzlement.
Utilize Freeze Drying Technique that organic matter is just preserved and arisen at the historic moment in order to adapt to the needs in market, Freeze Drying Technique is that wet stock or solution are frozen into solid-state at lower temperature (10 DEG C～-50 DEG C), then under vacuum (1.3～13 handkerchief), make moisture wherein be directly sublimed into gaseous state without liquid state, finally make the technology of material dewatering, the dried product of obtaining is encapsulated and stored, make dried product and airborne oxygen and steam isolated, now goods can be placed under normal temperature and preserve, place and energy dependence are reduced greatly, in the time that needs use, being carried out rehydration uses again, the method provides great power-assisted to organic preservation.
Summary of the invention
For above-mentioned situation, for overcoming the defect of prior art, the present invention's object is just to provide store method and the device of a kind of animal body/plant tissue, cell, organ and body fluid, solves the large problem of retain costs.
Its technical scheme is as follows:
The protection device of a kind of animal body/plant tissue, cell, organ and body fluid, comprise appearance body, hold body upper end and be provided with drain hole, in drain hole, filling has the sealing-plug with exhaust tube, the length of drain hole is longer than the length of sealing-plug, exhaust tube is connected with appearance body inside by the passage being arranged on sealing-plug, and exhaust tube is provided with isolated body and the outside control valve being communicated with of holding, and the upper end of exhaust tube is provided with the connector for being connected with air-extractor.
Another aspect of the present invention, provides the store method of a kind of animal body/plant tissue, cell, organ and body fluid, the steps include:
1, animal body/plant tissue, cell, organ and body fluid and the save set of the present invention needs preserved carry out sterilization processing.
2, in time animal body/plant tissue after treatment, cell, organ and body fluid are packed in save set of the present invention, and holding device sealing.
3, the save set that animal body/plant tissue, cell, organ and body fluid are housed is placed in the hothouse of freeze drier, open the control valve of save set and close rapidly hothouse, animal body/plant tissue, cell, organ and body fluid are carried out to freeze drying.
4, after freeze drying is processed, connect rapidly the bleeding point of air extractor by the exhaust tube connector on save set, save set is bled, and to make in the appearance body of save set be vacuum state, holds after body reaches vacuum state and close rapidly valve processed, prevents from holding in body, entering outside air.
5, the above-mentioned save set in vacuum state is taken out to freeze drier, utilize thermal-flame to carry out hot-melting sealed to the drain hole of sealing-plug lower end, thermal-flame melts drain hole form sealing, and drain hole upper end and sealing-plug wire drawing are removed under hot melt state at drain hole, make to hold body and removing the entirety that forms sealing behind drain hole upper end.
6, the save set of above-mentioned steps is placed under normal temperature state, preserve stand-by.
The present invention utilizes Freeze Drying Technique can keep animal body/plant tissue, cell, the bioactive technical characterstic of organ and body fluid is by animal body/plant tissue, cell, organ and body fluid carry out encapsulation process after freeze drying, make animal body/plant tissue, cell, organ and body fluid and outside air are isolated to be convenient to preserve under normal temperature state, can greatly reduce animal body/plant tissue compared with existing ultralow temperature Techniques of preserving, cell, the preservation condition of organ and body fluid and retain costs, to the promotion and application of the technology condition of providing convenience.
Brief description of the drawings
Fig. 1 is the front view of save set of the present invention.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the specific embodiment of the present invention is described in further details.
As shown in Figure 1, a kind of animal body/plant tissue, cell, organ and body fluid save set, comprise and hold body 1, hold body 1 upper end and be provided with drain hole 2, the interior filling of drain hole 2 has the sealing-plug 4 with exhaust tube 3, the length of drain hole 2 is longer than the length of sealing-plug 4, exhaust tube 3 is connected with appearance body 1 inside by the passage being arranged on sealing-plug, exhaust tube 3 is provided with isolated body 1 and the outside control valve 5 being communicated with of holding, and the upper end of exhaust tube 3 is provided with the connector 6 for being connected with air-extractor.
The bore of said drain hole adapts with needing animal body/plant tissue, cell and organ 7 volumes preserved.
Be convenient to observe for the animal body/plant tissue, cell, organ 7 and the body fluid that make to preserve, said appearance body is transparent glass material.
In the time that animal body/plant tissue, cell, organ and the body fluid 7 of required preservation need to keep in Dark Place, said appearance body is dense glass material.
Said sealing-plug, exhaust tube, control valve and connector are formed in one.
A kind of animal body/plant tissue, cell, organ and body fluid store method, the steps include:
1, animal body/plant tissue, cell, organ and body fluid and the save set of the present invention needs preserved carry out sterilization processing.
2, promptly animal body/plant tissue after treatment, cell, organ and body fluid official are packed in save set of the present invention, and holding device sealing.
3, the save set that animal body/plant tissue, cell, organ and body fluid are housed is placed in the hothouse of freeze drier, open the control valve of save set and close rapidly hothouse, animal body/plant tissue, cell, organ and body fluid are carried out to freeze drying.
4, after freeze drying is processed, connect rapidly the bleeding point of air extractor by the exhaust tube connector on save set, save set is bled, and to make in the appearance body of save set be vacuum state, holds after body reaches vacuum state and tighten rapidly control valve, prevents from holding in body, entering outside air.
5, the above-mentioned save set in vacuum state is taken out to freeze drier, utilize thermal-flame to carry out hot-melting sealed to the drain hole of sealing-plug lower end, thermal-flame melts drain hole form sealing, and drain hole upper end and sealing-plug wire drawing are removed under hot melt state at drain hole, make to hold body and removing the entirety that forms sealing behind drain hole upper end.
6, the save set of above-mentioned steps is placed under normal temperature state, preserve stand-by.
As preferred embodiment, in step 5, before drain hole is carried out to hot-melting sealed, the material of inert gas or enhancing preservation object activity can be put into and held body through exhaust tube, carry out again afterwards hot-melting sealed.
The present invention utilizes Freeze Drying Technique can keep animal body/plant tissue, cell, the bioactive technical characterstic of organ and body fluid is by animal body/plant tissue, cell, organ and body fluid carry out encapsulation process after freeze drying, make animal body/plant tissue, cell, organ and body fluid and outside air are isolated to be convenient to preserve under normal temperature state, can greatly reduce animal body/plant tissue compared with existing ultralow temperature Techniques of preserving, cell, the preservation condition of organ and body fluid and retain costs, to the promotion and application of the technology condition of providing convenience.
Above-described embodiment is described the preferred embodiment of the present invention; not scope of the present invention is limited; design under the prerequisite of spirit not departing from the present invention; various distortion and improvement that those of ordinary skill in the art make technical scheme of the present invention, all should fall in the definite protection domain of the claims in the present invention book.
Claims (7)
1. animal body/plant tissue, cell, organ and body fluid save set, comprise and hold body (1), hold body (1) upper end and be provided with drain hole (2), it is characterized in that, in drain hole (2), filling has the sealing-plug (4) with exhaust tube (3), exhaust tube (3) is connected with appearance body (1) inside by the passage being arranged on sealing-plug, exhaust tube (3) is provided with isolated body (1) and the outside control valve (5) being communicated with of holding, and the upper end of exhaust tube (3) is provided with the connector (6) for being connected with air-extractor.
2. animal body/plant tissue according to claim 1, cell, organ and body fluid save set, it is characterized in that, the bore of said drain hole (2) adapts with needing animal body/plant tissue, cell, organ and body fluid (7) volume/quantity of preserving.
3. animal body/plant tissue according to claim 1, cell, organ and body fluid save set, it is characterized in that, be convenient to observe for the animal body/plant tissue, cell, organ and the body fluid (7) that make to preserve, said appearance body is transparent glass material.
4. animal body/plant tissue according to claim 1, cell, organ and body fluid save set, it is characterized in that, in the time that animal body/plant tissue, cell, organ and the body fluid (7) of required preservation need to keep in Dark Place, said appearance body (1) is opaque or coloured glass material.
5. animal body/plant tissue according to claim 1, cell, organ and body fluid save set, is characterized in that, said sealing-plug (4), exhaust tube (3), control valve (5) and connector (6) are formed in one.
6. right to use requires a store method for animal body/plant tissue, cell, organ and the body fluid of 1 to 5 arbitrary described save set, the steps include:
Step 1, animal body/plant tissue, cell, organ and body fluid and save set of the present invention that needs are preserved carry out sterilization processing;
Step 2, animal body/plant tissue after treatment, cell, organ and body fluid are packed in described save set in time, and holding device sealing;
Step 3, the save set that animal body/plant tissue, cell, organ and body fluid are housed is placed in the hothouse of freeze drier, open the control valve of save set and close rapidly hothouse, animal body/plant tissue, cell, organ and body fluid are carried out to freeze drying;
After step 4, freeze drying are processed, connect rapidly the bleeding point of air extractor by the exhaust tube connector on save set, save set is bled, and to make in the appearance body of save set be vacuum state, hold body and tighten rapidly control valve after reaching vacuum state, prevent from holding in body, entering outside air;
Step 5, the above-mentioned save set in vacuum state is taken out to freeze drier, utilize thermal-flame to carry out hot-melting sealed to the drain hole of sealing-plug lower end, thermal-flame melts drain hole form sealing, and drain hole upper end and sealing-plug wire drawing are removed under hot melt state at drain hole, make to hold body and removing the entirety that forms sealing behind drain hole upper end;
Step 6, by the save set described in claim 1 to 5 in the present invention be placed under normal temperature state, preserve stand-by.
7. animal body/plant tissue according to claim 6, cell, organ and body fluid store method, it is characterized in that, in step 5 before drain hole is carried out to hot-melting sealed, the material of inert gas or enhancing preservation object activity can be put into and held body through exhaust tube, carry out again afterwards hot-melting sealed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410599448.XA CN104365580B (en) | 2014-10-31 | 2014-10-31 | Store method and the device of animal body/plant tissue, cell, organ and body fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410599448.XA CN104365580B (en) | 2014-10-31 | 2014-10-31 | Store method and the device of animal body/plant tissue, cell, organ and body fluid |
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| Publication Number | Publication Date |
|---|---|
| CN104365580A CN104365580A (en) | 2015-02-25 |
| CN104365580B true CN104365580B (en) | 2016-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410599448.XA Active CN104365580B (en) | 2014-10-31 | 2014-10-31 | Store method and the device of animal body/plant tissue, cell, organ and body fluid |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105501631B (en) * | 2015-12-31 | 2019-02-15 | 苏州奥特科然医疗科技有限公司 | A kind of packing container and its application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2456692Y (en) * | 2000-11-06 | 2001-10-31 | 蔡士香 | Filter type negative pressure bottle |
| CN201256973Y (en) * | 2008-09-28 | 2009-06-17 | 贺爱荣 | Finger control type body-liquid automatic sucker |
| CN202107497U (en) * | 2011-05-31 | 2012-01-11 | 武汉华龙生物制药有限公司 | Liquid medicine reverse suction preventive device for vacuum stopper suction pipeline of stopper adding machine |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8231027B2 (en) * | 2007-11-09 | 2012-07-31 | Jui Te Wang | Vacuum container having air release valve cover |
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2014
- 2014-10-31 CN CN201410599448.XA patent/CN104365580B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2456692Y (en) * | 2000-11-06 | 2001-10-31 | 蔡士香 | Filter type negative pressure bottle |
| CN201256973Y (en) * | 2008-09-28 | 2009-06-17 | 贺爱荣 | Finger control type body-liquid automatic sucker |
| CN202107497U (en) * | 2011-05-31 | 2012-01-11 | 武汉华龙生物制药有限公司 | Liquid medicine reverse suction preventive device for vacuum stopper suction pipeline of stopper adding machine |
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| CN104365580A (en) | 2015-02-25 |
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