CN104365580A - Method and device for preserving tissues, cells, organs and body fluid of animal body or plant body - Google Patents
Method and device for preserving tissues, cells, organs and body fluid of animal body or plant body Download PDFInfo
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- CN104365580A CN104365580A CN201410599448.XA CN201410599448A CN104365580A CN 104365580 A CN104365580 A CN 104365580A CN 201410599448 A CN201410599448 A CN 201410599448A CN 104365580 A CN104365580 A CN 104365580A
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Abstract
The invention relates to a method and device for preserving tissues, cells, organs and body fluid of an animal body or a plant body. The method comprises the steps of putting disinfected tissues, cells and organs of the animal body or the plant body into a disinfected preservation device; then, putting the preservation device into a freezing dryer, and carrying out freeze drying on the preservation device; then, vacuumizing the preservation device; carrying out hot melt sealing on a discharge hole of the preservation device while the preservation device is maintained to be in a vacuum state, and removing a melt at the upper end of the discharge hole and a sealing plug in a wire-drawing way to enable the melt to become a sealed whole; then, storing the melt for a long time. The tissues, cells and organs of the animal body or the plant body are treated in a sealing way after being treated by freeze drying, so that the tissues, cells and organs of the animal body or the plant body are isolated from the air of the outside so as to be conveniently stored at the room temperature, the preservation conditions and preservation cost of the tissues, cells and organs of the animal body or the plant body are greatly reduced, and convenience is provided for promotion and application of the technology.
Description
Technical field
The present invention relates to the preservation field of animal and plant body tissue and organ and body fluid, particularly relate to store method and the device of a kind of animal body/plant corpus tissue, cell, organ and body fluid.
Background technology
At biology, in medical science and field of food, organic preservation is the problem that people pay close attention to always, particularly in clinical medicine and biological field, animal body/plant corpus tissue, cell, organ and body fluid often need preserve and keep biologically active for a long time, in order to anti-bacteria and biology enzyme biochemical reaction extend the holding time in process, usual employing Excised Embryos technology, but organism, tissue and organ are very easily being lowered the temperature and damaged and loss of biological activity in rewarming process, very high to the requirement of technology, and lower the temperature complete preservation and rewarming process are also a major challenge of technology, the process of particularly preserving, because organic matter may need to preserve the longer time, keep its biologically active just uninterrupted low-temperature condition keeping organism of needs, so just to having higher requirement with the energy supply of preserving ground with preserving, its retain costs corresponding can increase gradually along with the growth of holding time, certain puzzlement is caused to the promotion and application of this technology.
Just arise at the historic moment in order to the needs adapting to market utilize Freeze Drying Technique to preserve organic matter, Freeze Drying Technique is frozen into solid-state at lower temperature (-10 DEG C ~-50 DEG C) by wet stock or solution, then under vacuum (1.3 ~ 13 handkerchief), moisture is wherein made to be directly sublimed into gaseous state without liquid state, finally make the technology of material dewatering, the dried product obtained is encapsulated and stored, oxygen in dried product and air and steam are completely cut off, now goods are preserved under can being placed in normal temperature, place and energy dependence are reduced greatly, when needs use, carried out rehydration again to use, the method provides great power-assisted to organic preservation.
Summary of the invention
For above-mentioned situation, for overcoming the defect of prior art, the object of the present invention is just to provide store method and the device of a kind of animal body/plant corpus tissue, cell, organ and body fluid, solves the problem that retain costs is large.
Its technical scheme is as follows:
The protection device of a kind of animal body/plant corpus tissue, cell, organ and body fluid, comprise appearance body, hold body upper end and be provided with drain hole, in drain hole, filling has the sealing-plug with exhaust tube, the length of drain hole is longer than the length of sealing-plug, exhaust tube is connected with appearance body inside by the ventilation hole be arranged on sealing-plug, and exhaust tube is provided with the isolated control valve holding body and ft connection, and the upper end of exhaust tube is provided with the connector for being connected with air-extractor.
Another aspect of the present invention, provides the store method of a kind of animal body/plant corpus tissue, cell, organ and body fluid, the steps include:
1, the animal body needing to preserve/plant corpus tissue, cell, organ and body fluid and save set of the present invention are carried out sterilization process.
2, in time the animal body after process/plant corpus tissue, cell, organ and body fluid is loaded in save set of the present invention, and holding device sealing.
3, the save set that animal body/plant corpus tissue, cell, organ and body fluid is housed is placed in the drying room of freeze drier, open the control valve of save set and close drying room rapidly, freeze drying is carried out to animal body/plant corpus tissue, cell, organ and body fluid.
4, after freeze drying process, the bleeding point of air extractor is connected rapidly by the exhaust tube connector on save set, bleeding to save set in the appearance body making save set is vacuum state, holds after body reaches vacuum state and closes valve processed rapidly, prevents from entering outside air in appearance body.
5, the above-mentioned save set being in vacuum state is taken out freeze drier, thermal-flame is utilized to carry out hot-melting sealed to the drain hole of sealing-plug lower end, drain hole melts and forms sealing by thermal-flame, and under drain hole is in hot melt state, drain hole upper end and sealing-plug wire drawing are removed, make to hold the entirety that body forms sealing after removing drain hole upper end.
6, preservation is carried out under the save set of above-mentioned steps being placed in normal temperature state stand-by.
The present invention utilizes Freeze Drying Technique can keep animal body/plant corpus tissue, cell, the technical characterstic of organ and humoral bio activity is by animal body/plant corpus tissue, cell, organ and body fluid carry out encapsulation process after freeze drying, make animal body/plant corpus tissue, cell, organ and body fluid and outside air are preserved under being isolated and being convenient to normal temperature state, animal body/plant corpus tissue greatly can be reduced compared with existing Excised Embryos technology, cell, the preservation condition of organ and body fluid and retain costs, the promotion and application of technology are provided convenience condition.
Accompanying drawing explanation
Fig. 1 is the front view of save set of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the specific embodiment of the present invention is described in further details.
As shown in Figure 1, a kind of animal body/plant corpus tissue, cell, organ and body fluid save set, comprise and hold body 1, hold body 1 upper end and be provided with drain hole 2, in drain hole 2, filling has the sealing-plug 4 with exhaust tube 3, the length of drain hole 2 is longer than the length of sealing-plug 4, exhaust tube 3 is connected with appearance body 1 inside by the ventilation hole be arranged on sealing-plug, exhaust tube 3 is provided with the isolated control valve 5 holding body 1 and ft connection, and the upper end of exhaust tube 3 is provided with the connector 6 for being connected with air-extractor.
The bore of said drain hole and the animal body needing to preserve/plant corpus tissue, cell and organ 7 volume adapt.
For making animal body/plant corpus tissue, cell, organ 7 and the body fluid preserved be convenient to observe, said appearance body is transparent glass material.
When animal body/plant corpus tissue, cell, organ and the body fluid 7 of required preservation needs to keep in Dark Place, said appearance body is dense glass material.
Said sealing-plug, exhaust tube, control valve and connector are formed in one.
A kind of animal body/plant corpus tissue, cell, organ and body fluid store method, the steps include:
1, the animal body needing to preserve/plant corpus tissue, cell, organ and body fluid and save set of the present invention are carried out sterilization process.
2, promptly animal body/plant corpus the tissue after process, cell, organ and body fluid official are loaded in save set of the present invention, and holding device sealing.
3, the save set that animal body/plant corpus tissue, cell, organ and body fluid is housed is placed in the drying room of freeze drier, open the control valve of save set and close drying room rapidly, freeze drying is carried out to animal body/plant corpus tissue, cell, organ and body fluid.
4, after freeze drying process, the bleeding point of air extractor is connected rapidly by the exhaust tube connector on save set, bleeding to save set in the appearance body making save set is vacuum state, holds body and to reach after vacuum state tightening control valve rapidly, prevent from holding in body entering outside air.
5, the above-mentioned save set being in vacuum state is taken out freeze drier, thermal-flame is utilized to carry out hot-melting sealed to the drain hole of sealing-plug lower end, drain hole melts and forms sealing by thermal-flame, and under drain hole is in hot melt state, drain hole upper end and sealing-plug wire drawing are removed, make to hold the entirety that body forms sealing after removing drain hole upper end.
6, preservation is carried out under the save set of above-mentioned steps being placed in normal temperature state stand-by.
As preferred embodiment, in step 5 before hot-melting sealed is carried out to drain hole, the material of inert gas or enhancing preservation object activity can be put into appearance body through exhaust tube, carry out hot-melting sealed more afterwards.
The present invention utilizes Freeze Drying Technique can keep animal body/plant corpus tissue, cell, the technical characterstic of organ and humoral bio activity is by animal body/plant corpus tissue, cell, organ and body fluid carry out encapsulation process after freeze drying, make animal body/plant corpus tissue, cell, organ and body fluid and outside air are preserved under being isolated and being convenient to normal temperature state, animal body/plant corpus tissue greatly can be reduced compared with existing Excised Embryos technology, cell, the preservation condition of organ and body fluid and retain costs, the promotion and application of technology are provided convenience condition.
Above-described embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that those of ordinary skill in the art make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determines.
Claims (7)
1. an animal body/plant corpus tissue, cell, organ and body fluid save set, comprise and hold body (1), hold body (1) upper end and be provided with drain hole (2), it is characterized in that, in drain hole (2), filling has the sealing-plug (4) with exhaust tube (3), exhaust tube (3) is connected with appearance body (1) inside by the ventilation hole be arranged on sealing-plug, exhaust tube (3) is provided with the isolated control valve (5) holding body (1) and ft connection, and the upper end of exhaust tube (3) is provided with the connector (6) for being connected with air-extractor.
2. animal body according to claim 1/plant corpus tissue, cell, organ and body fluid save set, it is characterized in that, the bore of said drain hole (2) adapts with needing animal body/plant corpus tissue, cell, organ and body fluid (7) volume/quantity of preserving.
3. animal body according to claim 1/plant corpus tissue, cell, organ and body fluid save set, it is characterized in that, for making animal body/plant corpus tissue, cell, organ and the body fluid (7) preserved be convenient to observe, said appearance body is transparent glass material.
4. animal body according to claim 1/plant corpus tissue, cell, organ and body fluid save set, it is characterized in that, when the animal body/plant corpus tissue of required preservation, cell, organ and body fluid (7) need to keep in Dark Place, said appearance body (1) is opaque or coloured glass material.
5. animal body according to claim 1/plant corpus tissue, cell, organ and body fluid save set, is characterized in that, said sealing-plug (4), exhaust tube (3), control valve (5) and connector (6) are formed in one.
6. a store method for animal body/plant corpus tissue, cell, organ and body fluid, the steps include:
1, the animal body needing to preserve/plant corpus tissue, cell, organ and body fluid and save set of the present invention are carried out sterilization process;
2, in time the animal body after process/plant corpus tissue, cell, organ and body fluid is loaded in save set of the present invention, and holding device sealing;
3, the save set that animal body/plant corpus tissue, cell, organ and body fluid is housed is placed in the drying room of freeze drier, open the control valve of save set and close drying room rapidly, freeze drying is carried out to animal body/plant corpus tissue, cell, organ and body fluid;
4, after freeze drying process, the bleeding point of air extractor is connected rapidly by the exhaust tube connector on save set, bleeding to save set in the appearance body making save set is vacuum state, holds body and to reach after vacuum state tightening control valve rapidly, prevent from holding in body entering outside air;
5, the above-mentioned save set being in vacuum state is taken out freeze drier, thermal-flame is utilized to carry out hot-melting sealed to the drain hole of sealing-plug lower end, drain hole melts and forms sealing by thermal-flame, and under drain hole is in hot melt state, drain hole upper end and sealing-plug wire drawing are removed, make to hold the entirety that body forms sealing after removing drain hole upper end;
6, preservation is carried out under the save set of above-mentioned steps being placed in normal temperature state stand-by.
7. animal body according to claim 4/plant corpus tissue, cell, organ and body fluid store method, it is characterized in that, in step 5 before hot-melting sealed is carried out to drain hole, the material of inert gas or enhancing preservation object activity can be put into appearance body through exhaust tube, carry out hot-melting sealed more afterwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410599448.XA CN104365580B (en) | 2014-10-31 | 2014-10-31 | Store method and the device of animal body/plant tissue, cell, organ and body fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410599448.XA CN104365580B (en) | 2014-10-31 | 2014-10-31 | Store method and the device of animal body/plant tissue, cell, organ and body fluid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104365580A true CN104365580A (en) | 2015-02-25 |
| CN104365580B CN104365580B (en) | 2016-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410599448.XA Active CN104365580B (en) | 2014-10-31 | 2014-10-31 | Store method and the device of animal body/plant tissue, cell, organ and body fluid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105501631A (en) * | 2015-12-31 | 2016-04-20 | 苏州奥特科然医疗设备有限公司 | Packaging container and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2456692Y (en) * | 2000-11-06 | 2001-10-31 | 蔡士香 | Filter type negative pressure bottle |
| US20090120939A1 (en) * | 2007-11-09 | 2009-05-14 | Jui-Te Wang | Vacuum container having air release valve cover |
| CN201256973Y (en) * | 2008-09-28 | 2009-06-17 | 贺爱荣 | Finger control type body-liquid automatic sucker |
| CN202107497U (en) * | 2011-05-31 | 2012-01-11 | 武汉华龙生物制药有限公司 | Liquid medicine reverse suction preventive device for vacuum stopper suction pipeline of stopper adding machine |
-
2014
- 2014-10-31 CN CN201410599448.XA patent/CN104365580B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2456692Y (en) * | 2000-11-06 | 2001-10-31 | 蔡士香 | Filter type negative pressure bottle |
| US20090120939A1 (en) * | 2007-11-09 | 2009-05-14 | Jui-Te Wang | Vacuum container having air release valve cover |
| CN201256973Y (en) * | 2008-09-28 | 2009-06-17 | 贺爱荣 | Finger control type body-liquid automatic sucker |
| CN202107497U (en) * | 2011-05-31 | 2012-01-11 | 武汉华龙生物制药有限公司 | Liquid medicine reverse suction preventive device for vacuum stopper suction pipeline of stopper adding machine |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105501631A (en) * | 2015-12-31 | 2016-04-20 | 苏州奥特科然医疗设备有限公司 | Packaging container and application thereof |
| CN105501631B (en) * | 2015-12-31 | 2019-02-15 | 苏州奥特科然医疗科技有限公司 | A kind of packing container and its application |
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| Publication number | Publication date |
|---|---|
| CN104365580B (en) | 2016-06-15 |
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