CN205687925U - A kind of skin bacterium sampling test kit - Google Patents
A kind of skin bacterium sampling test kit Download PDFInfo
- Publication number
- CN205687925U CN205687925U CN201620504461.7U CN201620504461U CN205687925U CN 205687925 U CN205687925 U CN 205687925U CN 201620504461 U CN201620504461 U CN 201620504461U CN 205687925 U CN205687925 U CN 205687925U
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- CN
- China
- Prior art keywords
- test kit
- skin
- sampling
- cotton swab
- bacteria samples
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
This utility model relates to test kit of sampling, a kind of skin bacterium sampling test kit.Seal in test kit and be independently respectively arranged with bacteria samples collection accessory, bacteria samples preserves cryovial and cleaning skin wet tissue.This utility model has possessed utensil and reagent needed for whole sampling, convenience sample collection, expands sampling place to non-medical place simultaneously, it is simple to the experiment sample that radix is bigger is collected.And possess independent sterilization packaging, reduce cross-contamination between environmental pollution when transporting before using and preserve and sample donor.
Description
Technical field
This utility model relates to test kit of sampling, a kind of skin bacterium sampling test kit.
Background technology
Skin, as the organ of human body maximum, is also that human body contacts most organs with external environment, and human body is resisted especially
External mechanical damages, the first barrier of physicochemical irritation and biotic influence.Corresponding, skin also provides for micropopulation
Vast vivosphere.Although the horn cell of human skin itself effectively forms firm physical barriers, and its table
The multiple materials that can directly suppress pathogenic microorganism to encroach on such as the active oxygen of mask, antibacterial peptide, free fatty, but skin table
Face is still deposited or includes the microorganisms such as antibacterial, fungus, virus in a large number, and these microorganisms maintain delicate human skin
Ecological balance.But, when skin microenvironment changes, during microbial state treatment, then can cause red swelling of the skin, pruritus, pustule etc.
Disease, causes skin injury, endangers human health.The lighter's scar affects personal image, and severe one causes inflammation, complication,
Even threat to life.
Along with biological development, genomics gradually comes into the people visual field.The Human Genome Project is also like a raging fire
Carrying out, " the human microbial's genome " that be described as the mankind's the second genome gradually causes the concern of people.But human skin
The detection of microbial genome, but encounters problem, and first, opinions vary for the method for sampling, and unification does not effectively gather way,
Secondly skin sampling quantity is few, causes damage again through extracting, and the amount that can be obtained with genome is difficult to reach detection limit, and
Extracting after PCR amplification or culture medium are artificially cultivated again, have the most again the Preference of amplification, the antibacterial i.e. having is amplified
Many, have then expand few even disappeared by other bacteriostatic.Therefore, it is difficult to the ecology of reflection skin surface microorganism
Original appearance.Meanwhile, it is short of again simply from the Plant composition of macroscopic perspective microorganisms and original scale and the impact on human body thereof
And effective solution.
Utility model content
In order to solve the problems referred to above, this practicality provides a kind of skin bacterium sampling test kit.
For achieving the above object, the technical scheme that this utility model provides is:
A kind of skin bacterium sampling test kit, seals in test kit and independently is respectively arranged with bacteria samples and gathers accessory 1, carefully
Bacterium sample preservation cryovial 2, cleaning skin wet tissue 3 and for sealing the sealed membrane of preservation.
Described test kit outer layer is provided with independent sealed outer package layer 0.
Described bacteria samples gathers accessory 1 and includes cotton swab and centrifuge tube, wherein, holds for antibacterial collection in centrifuge tube
Sampling eluent.
Described cotton swab bar material is bamboo, and two ends are equipped with absorbent cotton head；Wherein, cotton swab one end distance apical head 1.5-
One it is provided with for the ring-type cut fractureed at 2cm.The a diameter of 5mm of cotton swab head.
It is the aseptic sample cryopreservation tube taking up freezing that described bacteria samples preserves cryovial；Wherein, cryopreservation tube is a length of can
Accommodate after sampling terminates at the ring-type cut that cotton swab fractures to cotton swab head so that it is after sampling, cotton swab is immersed in cryopreservation tube and is held
In the freezing liquid of dress.
This utility model is had an advantage in that
This utility model test kit can complete human skin's microorganism collection, and the most effectively preserves,
Avoid original state of microbial population and quantity is impacted, with preferably reaction skin surface micro-environmental state, for section
Learn research and medical diagnosis provides direct material basis, or as the follow-up raw material cultivating amplification.
Using test kit collection of the present utility model and store method simply effective, collection capacity is sufficient, thus solves phase
Few with the skin surface antibacterial collection capacity of oil partially to being dried, it is difficult to for the problem of subsequent gene group correlation molecule experiment.
Accompanying drawing explanation
The configuration schematic diagram of the skin bacterium sampling test kit that Fig. 1 provides for this utility model.
Detailed description of the invention
By following description and combine the detailed description that this utility model is carried out by institute's accompanying drawing, hereinafter, will be with reference to attached
Figure is more fully described the preferred implementation of the present utility model realizing above-mentioned purpose.
As it is shown in figure 1, skin bacterium sampling test kit, the interior sealing of test kit is independently respectively arranged with bacteria samples collection and joins
Part 1, bacteria samples preserve cryovial 2 and cleaning skin wet tissue 3.Described test kit outer layer is provided with independent sealed outer package layer 0.
Described bacteria samples gathers accessory 1 and includes cotton swab and centrifuge tube, wherein, holds for antibacterial collection in centrifuge tube
Sampling eluent.Described centrifuge tube can be the centrifuge tube of 1.5ml or 0.5ml, takes up the eluting of its 2/3 volume the most to greatest extent
Liquid
Described sampling eluent can obtain in the composition configuration as described in prior art, or configures according to following compositions,
By volume percentages, tween 20 is 0.1%；Potassium dihydrogen phosphate is 0.027%；Dipotassium hydrogen phosphate is 0.142%；Sodium chloride
It is 0.9%；Potassium chloride is 0.002%, and surplus is water, carries out sterilizing after mixing.
Described cotton swab bar material is bamboo, and two ends are equipped with absorbent cotton head；Wherein, cotton swab one end distance apical head 1.5-
One it is provided with for the ring-type cut fractureed at 2cm.The a diameter of 5mm of cotton swab head.
It is the aseptic sample cryopreservation tube taking up freezing that described bacteria samples preserves cryovial；Wherein, cryopreservation tube is a length of can
Accommodate sampling terminate after at the ring-type cut that cotton swab fractures to cotton swab head so that it is during after sampling, cotton swab is immersed in freezing liquid.
Described freezing liquid can obtain in the composition configuration as described in prior art, or configures according to following compositions, by body
Long-pending percentages, glycerol 30%-50%, potassium dihydrogen phosphate is 0.027%；Dipotassium hydrogen phosphate is 0.142%；Sodium chloride is
0.9%；Potassium chloride is 0.002%, and surplus is water, carries out sterilizing after mixing.Described bacteria samples preserves outside cryovial
With the sealed membrane of 1cm width 4cm length in packaging.Further, cryopreservation tube pipe cap top and pipe shaft lateral parts are matte surface, just
In carrying out dualism number labelling.
Described cleaning skin wet tissue is for cleaning skin and to alleviate the nursing wet tissue that skin is uncomfortable, is and includes 75%
The non-woven fabrics of ethanol.
Concrete sampling flow process is:
A) implement sampler and wear disposable glove, remove sample collection accessory outer package, open 0.5ml centrifuge tube, will
Thered is provided aseptic cotton carrier picks the sample solution in 0.5ml centrifuge tube pipe；B) use has picked the cotton swab head of eluent, firmly wipes
Wiping the skin part of this required person of being sampled that samples, wherein, degree firmly is for can bear with the person of being sampled and keeping
On the premise of cotton swab is complete at utmost；
When c) carrying out wiping, needing to rotate cotton swab side polished, wiping direction should keep by center to both sides or by closely
The person's of being sampled end to far-end wiping, can not wiping back and forth, meanwhile, skin injury position need to be avoided.
D) the wiping time should keep 2-5 minute, to guarantee to reach sampling quantity, for molecule experiments or the particular sample degree of depth
Time, the time of wiping should extend, the longest can not be super many 10 minutes.
After sampling terminates, by cotton swab head at folding line shown in cotton swab bar, the sample in 2ml sample cryopreservation tube that fractures is protected
In liquid storage, build lid, seal up sealed membrane, the sample cryopreservation tube being filled with sample is stored in-20 DEG C, if long-term preservation, protect
It is stored in-80 DEG C of environment.
Claims (3)
1. a skin bacterium sampling test kit, it is characterised in that: the interior sealing of test kit is independently respectively arranged with bacteria samples and adopts
Collection accessory (1), bacteria samples preserve cryovial (2) and cleaning skin wet tissue (3)；
Described bacteria samples gathers accessory (1) and includes cotton swab and centrifuge tube, wherein, holds for adopting that antibacterial is collected in centrifuge tube
Sample eluent.
2. the skin bacterium sampling test kit as described in claim 1, it is characterised in that: described test kit outer layer is provided with independent close
The outer package layer of envelope.
3. the skin bacterium sampling test kit as described in claim 1, it is characterised in that: the pole stock matter of described cotton swab is bamboo, two
End is equipped with absorbent cotton head；Wherein, it is provided with one for the ring-type cut fractureed at cotton swab one end distance apical head 1.5-2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620504461.7U CN205687925U (en) | 2016-05-27 | 2016-05-27 | A kind of skin bacterium sampling test kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620504461.7U CN205687925U (en) | 2016-05-27 | 2016-05-27 | A kind of skin bacterium sampling test kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205687925U true CN205687925U (en) | 2016-11-16 |
Family
ID=57263309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620504461.7U Expired - Fee Related CN205687925U (en) | 2016-05-27 | 2016-05-27 | A kind of skin bacterium sampling test kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205687925U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110269644A (en) * | 2019-05-24 | 2019-09-24 | 天益健康科学研究院（镇江）有限公司 | A kind of genital tract bacteria detection specimen sample method |
| CN111656156A (en) * | 2018-01-30 | 2020-09-11 | 善肴控股株式会社 | Method for trapping microorganism and gene and method for extracting the same |
| CN111996233A (en) * | 2020-07-27 | 2020-11-27 | 天津科技大学 | Microbe sampling method for facial skin surface based on metagenomics and application |
-
2016
- 2016-05-27 CN CN201620504461.7U patent/CN205687925U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111656156A (en) * | 2018-01-30 | 2020-09-11 | 善肴控股株式会社 | Method for trapping microorganism and gene and method for extracting the same |
| CN110269644A (en) * | 2019-05-24 | 2019-09-24 | 天益健康科学研究院（镇江）有限公司 | A kind of genital tract bacteria detection specimen sample method |
| CN111996233A (en) * | 2020-07-27 | 2020-11-27 | 天津科技大学 | Microbe sampling method for facial skin surface based on metagenomics and application |
| WO2022021927A1 (en) * | 2020-07-27 | 2022-02-03 | 天津科技大学 | Metagenomics-based method for sampling microorganism on facial skin surface, and application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161116 Termination date: 20180527 |