CN104364263B - 含有层粘连蛋白和钙粘蛋白的细胞培养基底 - Google Patents

含有层粘连蛋白和钙粘蛋白的细胞培养基底 Download PDF

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CN104364263B
CN104364263B CN201280057413.2A CN201280057413A CN104364263B CN 104364263 B CN104364263 B CN 104364263B CN 201280057413 A CN201280057413 A CN 201280057413A CN 104364263 B CN104364263 B CN 104364263B
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laminin
cells
cadherin
cell
albumin
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CN104364263A (zh
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卡尔·特吕格瓦松
谢尔盖·罗丁
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Reproductive Health (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Sustainable Development (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201280057413.2A 2011-09-22 2012-09-19 含有层粘连蛋白和钙粘蛋白的细胞培养基底 Active CN104364263B (zh)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US201161537940P 2011-09-22 2011-09-22
US61/537,940 2011-09-22
US201161565380P 2011-11-30 2011-11-30
US61/565,380 2011-11-30
US201161565849P 2011-12-01 2011-12-01
US61/565,849 2011-12-01
US201261624588P 2012-04-16 2012-04-16
US61/624,588 2012-04-16
US201261625321P 2012-04-17 2012-04-17
US61/625,321 2012-04-17
PCT/IB2012/002214 WO2013041961A1 (en) 2011-09-22 2012-09-19 Cell culture substrate comprising a laminin and a cadherin

Publications (2)

Publication Number Publication Date
CN104364263A CN104364263A (zh) 2015-02-18
CN104364263B true CN104364263B (zh) 2018-06-29

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US (1) US10240124B2 (cg-RX-API-DMAC7.html)
EP (2) EP2758427B1 (cg-RX-API-DMAC7.html)
JP (1) JP6049733B2 (cg-RX-API-DMAC7.html)
KR (1) KR102048464B1 (cg-RX-API-DMAC7.html)
CN (1) CN104364263B (cg-RX-API-DMAC7.html)
AU (2) AU2012311233B2 (cg-RX-API-DMAC7.html)
CA (1) CA2849244C (cg-RX-API-DMAC7.html)
DK (1) DK3144319T3 (cg-RX-API-DMAC7.html)
IL (1) IL231630B (cg-RX-API-DMAC7.html)
SG (2) SG10201704803RA (cg-RX-API-DMAC7.html)
WO (1) WO2013041961A1 (cg-RX-API-DMAC7.html)

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IL281453B (en) 2009-11-17 2022-07-01 Astellas Inst For Regenerative Medicine Methods for preparing human rpe cells and pharmaceutical preparations of human rpe cells
WO2013156855A1 (en) * 2012-04-20 2013-10-24 Biolamina Ab Maintenance of differentiated cells with laminins
AU2013287307A1 (en) * 2012-07-12 2015-02-26 Isletone Ab Derivation and self-renewal of IsI1+ cells and uses thereof
WO2014037807A2 (en) * 2012-09-07 2014-03-13 Biolamina Ab Stem cell bank
US10287541B2 (en) * 2013-06-12 2019-05-14 Osaka University Cell culture vessel coated with laminin fragment in dry state
US10738323B2 (en) 2013-07-12 2020-08-11 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
SMT201900005T1 (it) * 2015-02-20 2019-05-10 Inst Nat Sante Rech Med Uso di una laminina per la differenziazione di cellule pluripotenti in cellule di lignaggio epatocitario
US10221395B2 (en) 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
EP3492592B1 (en) * 2016-07-29 2024-11-06 Takara Bio Inc. Fibronectin fragment to be used for stem cell production
US20190211305A1 (en) * 2016-08-25 2019-07-11 Kyoto University Method for Culturing Pluripotent Stem Cells on Specific Laminin
WO2018090084A1 (en) * 2016-11-16 2018-05-24 Cynata Therapeutics Limited Pluripotent stem cell assay
CN109125805A (zh) * 2018-09-06 2019-01-04 京和生殖医学技术（沈阳）有限公司 一种干细胞复合plga支架试剂盒及其应用

Citations (3)

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JP2006087396A (ja) * 2004-09-27 2006-04-06 National Institute For Environmental Studies 細胞培養基質及びその製造方法
WO2008084401A2 (en) * 2007-01-04 2008-07-17 Karl Tryggvason Composition and method for enabling proliferation of pluripotent stem cells
WO2010005995A1 (en) * 2008-07-07 2010-01-14 Mass Institute Of Technology Micropatterned co-culture systems as infectious disease analysis platforms

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JP2001508302A (ja) * 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
WO2002050111A2 (en) * 2000-12-21 2002-06-27 Biostratum, Inc. Isolated laminin 10
PT1572984E (pt) 2002-12-16 2016-06-03 Technion Res & Dev Foundation Sistema de cultura para células estaminais embrionárias humanas sem células alimentadoras e sem xeno
US10829733B2 (en) 2007-01-04 2020-11-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells
EP2559756B1 (en) 2007-07-01 2019-12-18 Janssen Biotech, Inc. Single pluripotent stem cell culture
AU2009322346B2 (en) * 2008-12-03 2015-07-02 The Scripps Research Institute Stem cell cultures
WO2010137746A1 (en) 2009-05-29 2010-12-02 Kyoto University Method for producing induced pluripotent stem cells and method for culturing the same
CA2707296A1 (en) 2009-06-12 2010-12-12 Richard T. Scott, Jr. Method for relative quantitation of chromosomal dna copy number in a single or few cells
KR101921350B1 (ko) * 2010-12-17 2018-11-22 바이오라미나 에이비 인간 다능성 줄기세포를 유지하기 위한 시스템
US9133266B2 (en) * 2011-05-06 2015-09-15 Wisconsin Alumni Research Foundation Vitronectin-derived cell culture substrate and uses thereof

Patent Citations (3)

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JP2006087396A (ja) * 2004-09-27 2006-04-06 National Institute For Environmental Studies 細胞培養基質及びその製造方法
WO2008084401A2 (en) * 2007-01-04 2008-07-17 Karl Tryggvason Composition and method for enabling proliferation of pluripotent stem cells
WO2010005995A1 (en) * 2008-07-07 2010-01-14 Mass Institute Of Technology Micropatterned co-culture systems as infectious disease analysis platforms

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Publication number Publication date
EP3144319B8 (en) 2019-07-03
IL231630B (en) 2019-10-31
CA2849244C (en) 2023-10-10
AU2012311233A1 (en) 2014-04-10
CN104364263A (zh) 2015-02-18
JP6049733B2 (ja) 2016-12-21
SG10201704803RA (en) 2017-07-28
AU2012311233B2 (en) 2017-03-16
CA2849244A1 (en) 2013-03-28
EP2758427A1 (en) 2014-07-30
EP3144319B1 (en) 2019-05-22
KR20140092819A (ko) 2014-07-24
KR102048464B1 (ko) 2019-11-25
US20140315306A1 (en) 2014-10-23
AU2017204108A1 (en) 2017-07-06
EP3144319A1 (en) 2017-03-22
US10240124B2 (en) 2019-03-26
IL231630A0 (en) 2014-05-28
WO2013041961A1 (en) 2013-03-28
SG11201400833XA (en) 2014-04-28
JP2014526271A (ja) 2014-10-06
EP2758427B1 (en) 2016-11-30
DK3144319T3 (da) 2019-08-05
AU2017204108B2 (en) 2018-11-15

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