CN104351258A - Compound biopesticide for preventing and curing rice sheath blight disease and preparation method thereof - Google Patents
Compound biopesticide for preventing and curing rice sheath blight disease and preparation method thereof Download PDFInfo
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- CN104351258A CN104351258A CN201410557569.8A CN201410557569A CN104351258A CN 104351258 A CN104351258 A CN 104351258A CN 201410557569 A CN201410557569 A CN 201410557569A CN 104351258 A CN104351258 A CN 104351258A
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- sheath blight
- rice sheath
- bacillus subtilis
- paecilomyces lilacinus
- agricultural chemicals
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Abstract
The invention discloses a compound biopesticide for preventing and curing a rice sheath blight disease and a preparation method thereof. The pesticide can improve the soil fertilizer efficiency at the same time and is a biopesticide with the effects of both a fertilizer and a pesticide. The pesticide is an aqueous solution or powder, the active ingredients in the aqueous solution or powder comprise bacillus subtilis B-2103 and paecilomyces lilacinus BS-003, and the weight ratio of the bacillus subtilis B-2103 to the bacterial strain of the paecilomyces lilacinus BS-003 is 1:(0.0005-1000). The bacillus subtilis B-2103 to the bacterial strain of the paecilomyces lilacinus BS-003 are compounded, the extracellular metabolite has an excellent effect of preventing the rice sheath blight disease and nitrobacteria, the bacterial strain of the paecilomyces lilacinus BS-003 play the synergistic effect, so that the rate of preventing and curing the rice sheath blight disease is more than 82%, meanwhile, the fertilizer efficiency of the soil is improved, and the utilization efficiency of the soil fertilizer N is strengthened.
Description
Technical field
The present invention relates to biopesticide and preparation method thereof, particularly a kind of complex biological agricultural chemicals and preparation method thereof of the water prevention rice sheath blight.
Background technology
Rice sheath blight disease is one of three large diseases of paddy rice, and it distributes extensively in the world, and harm is large, and this disease not only affects the output of paddy rice, but also has a strong impact on the quality of rice of paddy rice.So far this disease in Asia, America, the rice cultivation such as Africa country generally occur, have some prophylactico-therapeutic measuress for rice sheath blight disease.Consult pertinent literature, open (bulletin) number: the CN103396954A of Chinese patent, disclose a kind of biocontrol strains, biological organic fertilizer and preparation method thereof of prevention and control rice sheath blight disease, utilize Trichoderma asperellum strain 12 zymotic fluid to prepare a kind of bio-fertilizer, find that it is 70% to the control rate of rice sheath blight disease.Open (bulletin) number: the CN103719162A of Chinese patent, disclose a kind of medicine and production method thereof of the water prevention rice sheath blight, merge under utilizing Radix Stephaniae Cepharanthae root tuber extract and S-Ethyl ethylthio sulfonate and levogyrate alpha-terpineol normal temperature, find that medicine spray water rice sheath blight control rate reaches 70%.Being only reach 70% to the control rate of paddy rice literary composition rot above, is the biopesticide that effect is single.
Summary of the invention
The invention provides complex biological agricultural chemicals of a kind of water prevention rice sheath blight and preparation method thereof, this agricultural chemicals can improve soil fertility simultaneously, is the biopesticide of fertile medicine economic benefits and social benefits.
A kind of complex biological agricultural chemicals of the water prevention rice sheath blight, it is characterized in that the formulation of this agricultural chemicals is aqua or pulvis, in aqua or pulvis, active component is: bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003, bacillus subtilis B-2103 content is: 500-8000CFU/ μ L; Paecilomyces lilacinus BS-003 content is: 1000-10000CFU/ μ L.
The complex biological agricultural chemicals of described a kind of water prevention rice sheath blight, it is characterized in that the formulations of pesticide are aqua, in aqua, active component is: bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003, bacillus subtilis B-2103 content is: 1000-6000CFU/ μ L; Paecilomyces lilacinus BS-003 content is: 500-6000CFU/ μ L.
Described Paecilomyces lilacinus BS-003 is the active bacteria formulation obtained through fermentation, separation screening.
A kind of preparation method of complex biological agricultural chemicals of the water prevention rice sheath blight, it is characterized in that the pesticide material that the method adopts is bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain, the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain is 1: 0.0005-1000.
The preparation method of the complex biological agricultural chemicals of described a kind of water prevention rice sheath blight, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1: 0.005-0.01.
The preparation method of the complex biological agricultural chemicals of described a kind of water prevention rice sheath blight, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1: 0.05-0.1.
The preparation method of the complex biological agricultural chemicals of described a kind of water prevention rice sheath blight, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1: 0.2-1.
The preparation method of the complex biological agricultural chemicals of described a kind of water prevention rice sheath blight, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1: 5-50.
The preparation method of the complex biological agricultural chemicals of described a kind of water prevention rice sheath blight, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1: 60-100.
The preparation method of the complex biological agricultural chemicals of described a kind of water prevention rice sheath blight, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1: 100-300.
The present invention is compared with existing similar technique, and its significant beneficial effect is embodied in:
The present invention adopts Paecilomyces lilacinus BS-003 bacterial strain and bacillus subtilis B-2103 to be pesticide material, because the mechanism of action of these two kinds of biological raw materials is different, composite by the two, Extracellular metabolism is good to the preventive effect such as Rhizoctonia solani Kuhn and nitrifying bacteria, synergistic effect can be played, reduce dosage, reduce pesticide resistance and occur.More than 82% is reached to the control rate of rice sheath blight disease, fertilizer efficiency in soil can be improved simultaneously, strengthen the fertile utilization ratio of soil N.
The present invention is conducive to the protecting agriculture ecosystem; Be conducive to agricultural sustainable development, the agricultural chemicals lasting period is long, environmentally safe, noresidue.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, describe the percentage by weight that the percentage by weight related to is pure material.
Embodiment 1
A complex biological agricultural chemicals for the water prevention rice sheath blight, the formulation of this agricultural chemicals is aqua, and in aqua, active component and content are:
Bacillus subtilis B-2103:10000CFU/ μ L; Pale purple plan blue or green BS-003:10000CFU/ μ L.
A preparation method for the complex biological agricultural chemicals of the water prevention rice sheath blight, the pesticide material of employing is bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain, and its weight proportion is 1: 0.005-0.01.
Water prevention rice sheath blight pharmacy effect comparison sheet
Embodiment 2
A complex biological agricultural chemicals for the water prevention rice sheath blight, the formulation of this agricultural chemicals is aqua, and in aqua, active component and content are:
Bacillus subtilis B-2103:10000CFU/ μ L; Paecilomyces lilacinus BS-003:10000CFU/ μ L.
A preparation method for the complex biological agricultural chemicals of the water prevention rice sheath blight, the pesticide material of employing is bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain, and its weight proportion is for being 1: 0.05-0.1.
Water prevention rice sheath blight pharmacy effect comparison sheet
Embodiment 3
A complex biological agricultural chemicals for the water prevention rice sheath blight, the formulation of this agricultural chemicals is aqua, and in aqua, active component and content are:
Bacillus subtilis B-2103:10000CFU/ μ L; Paecilomyces lilacinus BS-003:10000CFU/ μ L.
A preparation method for the complex biological agricultural chemicals of the water prevention rice sheath blight, the pesticide material of employing is bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain, and its weight proportion is for being 1: 0.2-1.
Water prevention rice sheath blight pharmacy effect comparison sheet
Embodiment 4
A complex biological agricultural chemicals for the water prevention rice sheath blight, the formulation of this agricultural chemicals is aqua, and in aqua, active component and content are:
Bacillus subtilis B-2103:10000CFU/ μ L; Paecilomyces lilacinus BS-003:10000CFU/ μ L.
A preparation method for the complex biological agricultural chemicals of the water prevention rice sheath blight, the pesticide material of employing is bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain, and its weight proportion is for being 1: 5-50.
Water prevention rice sheath blight pharmacy effect comparison sheet
Embodiment 5
A complex biological agricultural chemicals for the water prevention rice sheath blight, the formulation of this agricultural chemicals is aqua, and in aqua, active component and content are:
Bacillus subtilis B-2103:10000CFU/ μ L; Paecilomyces lilacinus BS-003:10000CFU/ μ L.
A preparation method for the complex biological agricultural chemicals of the water prevention rice sheath blight, the pesticide material of employing is bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain, and its weight proportion is for being 1: 60-100.
Water prevention rice sheath blight pharmacy effect comparison sheet
Embodiment 6
A complex biological agricultural chemicals for the water prevention rice sheath blight, the formulation of this agricultural chemicals is aqua, and in aqua, active component and content are:
Bacillus subtilis B-2103:10000CFU/ μ L; Paecilomyces lilacinus BS-003:10000CFU/ μ L.
A preparation method for the complex biological agricultural chemicals of the water prevention rice sheath blight, the pesticide material of employing is bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain, and its weight proportion is for being 1: 100-300.
Water prevention rice sheath blight pharmacy effect comparison sheet
Claims (10)
1. the complex biological agricultural chemicals of a water prevention rice sheath blight, it is characterized in that the formulation of this agricultural chemicals is aqua or pulvis, in aqua or pulvis, active component is: bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003, bacillus subtilis B-2103 content is: 500-8000CFU/ μ L; Paecilomyces lilacinus BS-003 content is: 1000-10000CFU/ μ L.
2. the complex biological agricultural chemicals of a kind of water prevention rice sheath blight according to claim 1, it is characterized in that the formulations of pesticide are aqua, in aqua, active component is: bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003, bacillus subtilis B-2103 content is: 1000-6000CFU/ μ L; Pale purplely draft mould BS-003 content and be: 500-6000CFU/ μ L.
3. the complex biological agricultural chemicals of a kind of water prevention rice sheath blight according to claim 1, is characterized in that said Paecilomyces lilacinus BS-003 is the active bacteria formulation obtained through fermentation, separation screening.
4. the preparation method of the complex biological agricultural chemicals of a water prevention rice sheath blight, it is characterized in that the pesticide material that the method adopts is bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain, the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 bacterial strain is 1:0.0005-1000.
5. the preparation method of a kind of complex biological agricultural chemicals of the water prevention rice sheath blight according to claim 4, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:0.005-0.01.
6. the preparation method of a kind of complex biological agricultural chemicals of the water prevention rice sheath blight according to claim 4, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:0.05-0.1.
7. the preparation method of a kind of complex biological agricultural chemicals of the water prevention rice sheath blight according to claim 4, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:0.2-1.
8. the preparation method of a kind of complex biological agricultural chemicals of the water prevention rice sheath blight according to claim 4, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:5-50.
9. the preparation method of a kind of complex biological agricultural chemicals of the water prevention rice sheath blight according to claim 4, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:60-100.
10. the preparation method of a kind of complex biological agricultural chemicals of the water prevention rice sheath blight according to claim 4, is characterized in that the weight proportion of bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:100-300.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410557569.8A CN104351258A (en) | 2014-10-20 | 2014-10-20 | Compound biopesticide for preventing and curing rice sheath blight disease and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410557569.8A CN104351258A (en) | 2014-10-20 | 2014-10-20 | Compound biopesticide for preventing and curing rice sheath blight disease and preparation method thereof |
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| CN104351258A true CN104351258A (en) | 2015-02-18 |
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| CN201410557569.8A Pending CN104351258A (en) | 2014-10-20 | 2014-10-20 | Compound biopesticide for preventing and curing rice sheath blight disease and preparation method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671210A (en) * | 2009-10-29 | 2010-03-17 | 福建省农业科学院农业生物资源研究所 | Preparation method of paecilomyces lilacinus biological fertilizer |
| CN101857848A (en) * | 2010-05-10 | 2010-10-13 | 华中农业大学 | Biocontrol bacillus subtilis WJ-1 for rice sheath blight, microbial inoculum and application thereof |
| CN101935628A (en) * | 2010-06-13 | 2011-01-05 | 浙江大学 | Bacillus subtilis and application thereof |
| CN102433282A (en) * | 2011-12-16 | 2012-05-02 | 华南农业大学 | Bacillus subtilis NB12, as well as culture method and application thereof |
| WO2014086752A1 (en) * | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising biological control agents |
-
2014
- 2014-10-20 CN CN201410557569.8A patent/CN104351258A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671210A (en) * | 2009-10-29 | 2010-03-17 | 福建省农业科学院农业生物资源研究所 | Preparation method of paecilomyces lilacinus biological fertilizer |
| CN101857848A (en) * | 2010-05-10 | 2010-10-13 | 华中农业大学 | Biocontrol bacillus subtilis WJ-1 for rice sheath blight, microbial inoculum and application thereof |
| CN101935628A (en) * | 2010-06-13 | 2011-01-05 | 浙江大学 | Bacillus subtilis and application thereof |
| CN102433282A (en) * | 2011-12-16 | 2012-05-02 | 华南农业大学 | Bacillus subtilis NB12, as well as culture method and application thereof |
| WO2014086752A1 (en) * | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising biological control agents |
Non-Patent Citations (1)
| Title |
|---|
| 张家家: "生防菌相容性测定方法比较及不同菌株混和对黄瓜生长的影响", 《中国植物保护学会第十一次全国会员代表大会暨2013年学术年会论文集》 * |
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